                         SEQUENCE LISTING

<110>  GlaxoSmithKline Biologicals S.A
 
<120>  Novel Influenza Antigens

<130>  VB65902

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  588
<212>  PRT
<213>  Artificial

<220>
<223>  recombinant H5 haemagglutinin (ECD-TMD-foldon) Indo05

<400>  1

Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
1               5                   10                  15      


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
            20                  25                  30          


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
        35                  40                  45              


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
    50                  55                  60                  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Lys Ala Asn Pro Thr Asn Asp Leu Cys Tyr Pro Gly Ser Phe Asn 
                85                  90                  95      


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
            100                 105                 110         


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser 
        115                 120                 125             


Ser Gly Asx Ser Ser Ala Cys Pro Tyr Leu Gly Ser Pro Ser Phe Phe 
    130                 135                 140                 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Thr Tyr Pro Thr Ile 
145                 150                 155                 160 


Lys Lys Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
                165                 170                 175     


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln 
            180                 185                 190         


Asn Pro Thr Thr Tyr Ile Ser Ile Gly Thr Ser Thr Leu Asn Gln Arg 
        195                 200                 205             


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
    210                 215                 220                 


Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
225                 230                 235                 240 


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
                245                 250                 255     


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Gly 
            260                 265                 270         


Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser 
        275                 280                 285             


Pro Met Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
    290                 295                 300                 


Tyr Asx Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
305                 310                 315                 320 


Pro Gln Arg Glu Ser Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile 
                325                 330                 335     


Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr 
            340                 345                 350         


Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys 
        355                 360                 365             


Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser 
    370                 375                 380                 


Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe 
385                 390                 395                 400 


Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp 
                405                 410                 415     


Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met 
            420                 425                 430         


Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu 
        435                 440                 445             


Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly 
    450                 455                 460                 


Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu 
465                 470                 475                 480 


Ser Ile Arg Asn Gly Thr Tyr Asn Tyr Pro Gln Tyr Ser Glu Glu Ala 
                485                 490                 495     


Arg Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly 
            500                 505                 510         


Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala 
        515                 520                 525             


Leu Ala Ile Met Met Ala Gly Leu Ser Leu Trp Met Cys Ser Ser Gly 
    530                 535                 540                 


Arg Leu Val Pro Arg Gly Ser Pro Gly Ser Gly Tyr Ile Pro Glu Ala 
545                 550                 555                 560 


Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu 
                565                 570                 575     


Leu Ser Thr Phe Leu Gly His His His His His His 
            580                 585             


<210>  2
<211>  1776
<212>  DNA
<213>  Artificial

<220>
<223>  recombinant H5 haemagglutinin (ECD-TMD-foldon)

<400>  2
gatcccgggg atcagatttg cattggttac catgcaaaca attcaacaga gcaggttgac       60

acaatcatgg aaaagaacgt tactgttaca catgcccaag acatactgga aaagacacac      120

aacgggaagc tctgcgatct agatggagtg aagcctctaa ttttaagaga ttgtagtgta      180

gctggatggc tcctcgggaa cccaatgtgt gacgaattca tcaatgtacc ggaatggtct      240

tacatagtgg agaaggccaa tccaaccaat gacctctgtt acccagggag tttcaacgac      300

tatgaagaac tgaaacacct attgagcaga ataaaccatt ttgagaaaat tcaaatcatc      360

cccaaaagtt cttggtccga tcatgaagcc tcatcaggag tgagctcagc atgtccatac      420

ctgggaagtc cctccttttt tagaaatgtg gtatggctta tcaaaaagaa cagtacatac      480

ccaacaataa agaaaagcta caataatacc aaccaagaag atcttttggt actgtgggga      540

attcaccatc ctaatgatgc ggcagagcag acaaggctat atcaaaaccc aaccacctat      600

atttccattg ggacatcaac actaaaccag agattggtac caaaaatagc tactagatcc      660

aaagtaaacg ggcaaagtgg aaggatggag ttcttctgga caattttaaa acctaatgat      720

gcaatcaact tcgagagtaa tggaaatttc attgctccag aatatgcata caaaattgtc      780

aagaaagggg actcagcaat tatgaaaagt gaattggaat atggtaactg caacaccaag      840

tgtcaaactc caatgggggc gataaactct agtatgccat tccacaacat acaccctctc      900

accatcgggg aatgccccaa atatgtgaaa tcaaacagat tagtccttgc aacagggctc      960

agaaatagcc ctcaaagaga gagcagaaga aaaaagagag gactatttgg agctatagca     1020

ggttttatag agggaggatg gcagggaatg gtagatggtt ggtatgggta ccaccatagc     1080

aatgagcagg ggagtgggta cgctgcagac aaagaatcca ctcaaaaggc aatagatgga     1140

gtcaccaata aggtcaactc aatcattgac aaaatgaaca ctcagtttga ggccgttgga     1200

agggaattta ataacttaga aaggagaata gagaatttaa acaagaagat ggaagacggg     1260

tttctagatg tctggactta taatgccgaa cttctggttc tcatggaaaa tgagagaact     1320

ctagactttc atgactcaaa tgttaagaac ctctacgaca aggtccgact acagcttagg     1380

gataatgcaa aggagctggg taacggttgt ttcgagttct atcacaaatg tgataatgaa     1440

tgtatggaaa gtataagaaa cggaacgtac aactatccgc agtattcaga agaagcaaga     1500

ctaaaaagag aggaaataag tggggtaaaa ttggaatcaa taggaactta ccaaatactg     1560

tcaatttatt caacagtggc gagttcccta gcactggcaa tcatgatggc tggtctatct     1620

ttatggatgt gctccagcgg ccgcttggtc cctcgtggaa gcccaggctc cggctacatc     1680

cccgaggccc cgcgcgacgg ccaggcctac gtgcgcaagg acggcgagtg ggtgctgctg     1740

tccaccttcc tgggacatca tcatcatcat cattga                               1776


<210>  3
<211>  10
<212>  PRT
<213>  Influenza Virus

<400>  3

Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
1               5                   10  


<210>  4
<211>  30
<212>  DNA
<213>  Influenza virus

<400>  4
aatggatcgt tacaatgcag aatttgcatt                                        30


<210>  5
<211>  38
<212>  PRT
<213>  Influenza Virus

<400>  5

Gly Val Lys Leu Glu Ser Ile Gly Thr Tyr Gln Ile Leu Ser Ile Tyr 
1               5                   10                  15      


Ser Thr Val Ala Ser Ser Leu Ala Leu Ala Ile Met Met Ala Gly Leu 
            20                  25                  30          


Ser Leu Trp Met Cys Ser 
        35              


<210>  6
<211>  114
<212>  DNA
<213>  Influenza Virus

<400>  6
ggggtaaaat tggaatcaat aggaacttac caaatactgt caatttattc aacagtggcg       60

agttccctag cactggcaat catgatggct ggtctatctt tatggatgtg ctcc            114


<210>  7
<211>  504
<212>  PRT
<213>  Influenza Virus

<400>  7

Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
1               5                   10                  15      


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
            20                  25                  30          


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
        35                  40                  45              


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
    50                  55                  60                  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Lys Ala Asn Pro Thr Asn Asp Leu Cys Tyr Pro Gly Ser Phe Asn 
                85                  90                  95      


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
            100                 105                 110         


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser 
        115                 120                 125             


Ser Gly Asx Ser Ser Ala Cys Pro Tyr Leu Gly Ser Pro Ser Phe Phe 
    130                 135                 140                 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Thr Tyr Pro Thr Ile 
145                 150                 155                 160 


Lys Lys Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
                165                 170                 175     


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln 
            180                 185                 190         


Asn Pro Thr Thr Tyr Ile Ser Ile Gly Thr Ser Thr Leu Asn Gln Arg 
        195                 200                 205             


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
    210                 215                 220                 


Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
225                 230                 235                 240 


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
                245                 250                 255     


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Gly 
            260                 265                 270         


Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser 
        275                 280                 285             


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
    290                 295                 300                 


Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
305                 310                 315                 320 


Pro Gln Arg Glu Ser Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile 
                325                 330                 335     


Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr 
            340                 345                 350         


Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys 
        355                 360                 365             


Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser 
    370                 375                 380                 


Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe 
385                 390                 395                 400 


Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp 
                405                 410                 415     


Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met 
            420                 425                 430         


Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu 
        435                 440                 445             


Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly 
    450                 455                 460                 


Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu 
465                 470                 475                 480 


Ser Ile Arg Asn Gly Thr Tyr Asn Tyr Pro Gln Tyr Ser Glu Glu Ala 
                485                 490                 495     


Arg Leu Lys Arg Glu Glu Ile Ser 
            500                 


<210>  8
<211>  1521
<212>  DNA
<213>  Influenza Virus

<400>  8
gatcccgggg atcagatttg cattggttac catgcaaaca attcaacaga gcaggttgac       60

acaatcatgg aaaagaacgt tactgttaca catgcccaag acatactgga aaagacacac      120

aacgggaagc tctgcgatct agatggagtg aagcctctaa ttttaagaga ttgtagtgta      180

gctggatggc tcctcgggaa cccaatgtgt gacgaattca tcaatgtacc ggaatggtct      240

tacatagtgg agaaggccaa tccaaccaat gacctctgtt acccagggag tttcaacgac      300

tatgaagaac tgaaacacct attgagcaga ataaaccatt ttgagaaaat tcaaatcatc      360

cccaaaagtt cttggtccga tcatgaagcc tcatcaggag tgagctcagc atgtccatac      420

ctgggaagtc cctccttttt tagaaatgtg gtatggctta tcaaaaagaa cagtacatac      480

ccaacaataa agaaaagcta caataatacc aaccaagaag atcttttggt actgtgggga      540

attcaccatc ctaatgatgc ggcagagcag acaaggctat atcaaaaccc aaccacctat      600

atttccattg ggacatcaac actaaaccag agattggtac caaaaatagc tactagatcc      660

aaagtaaacg ggcaaagtgg aaggatggag ttcttctgga caattttaaa acctaatgat      720

gcaatcaact tcgagagtaa tggaaatttc attgctccag aatatgcata caaaattgtc      780

aagaaagggg actcagcaat tatgaaaagt gaattggaat atggtaactg caacaccaag      840

tgtcaaactc caatgggggc gataaactct agtatgccat tccacaacat acaccctctc      900

accatcgggg aatgccccaa atatgtgaaa tcaaacagat tagtccttgc aacagggctc      960

agaaatagcc ctcaaagaga gagcagaaga aaaaagagag gactatttgg agctatagca     1020

ggttttatag agggaggatg gcagggaatg gtagatggtt ggtatgggta ccaccatagc     1080

aatgagcagg ggagtgggta cgctgcagac aaagaatcca ctcaaaaggc aatagatgga     1140

gtcaccaata aggtcaactc aatcattgac aaaatgaaca ctcagtttga ggccgttgga     1200

agggaattta ataacttaga aaggagaata gagaatttaa acaagaagat ggaagacggg     1260

tttctagatg tctggactta taatgccgaa cttctggttc tcatggaaaa tgagagaact     1320

ctagactttc atgactcaaa tgttaagaac ctctacgaca aggtccgact acagcttagg     1380

gataatgcaa aggagctggg taacggttgt ttcgagttct atcacaaatg tgataatgaa     1440

tgtatggaaa gtataagaaa cggaacgtac aactatccgc agtattcaga agaagcaaga     1500

ctaaaaagag aggaaataag t                                               1521


<210>  9
<211>  29
<212>  PRT
<213>  Bacteriophage

<400>  9

Gly Ser Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val 
1               5                   10                  15      


Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
            20                  25                  


<210>  10
<211>  87
<212>  DNA
<213>  Bacteriophage

<400>  10
ggctccggct acatccccga ggccccgcgc gacggccagg cctacgtgcg caaggacggc       60

gagtgggtgc tgctgtccac cttcctg                                           87


<210>  11
<211>  564
<212>  PRT
<213>  Influenza Virus

<400>  11

Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
1               5                   10                  15      


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
            20                  25                  30          


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
        35                  40                  45              


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
    50                  55                  60                  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Lys Ala Asn Pro Thr Asn Asp Leu Cys Tyr Pro Gly Ser Phe Asn 
                85                  90                  95      


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
            100                 105                 110         


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser 
        115                 120                 125             


Ser Gly Asx Ser Ser Ala Cys Pro Tyr Leu Gly Ser Pro Ser Phe Phe 
    130                 135                 140                 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Thr Tyr Pro Thr Ile 
145                 150                 155                 160 


Lys Lys Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
                165                 170                 175     


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln 
            180                 185                 190         


Asn Pro Thr Thr Tyr Ile Ser Ile Gly Thr Ser Thr Leu Asn Gln Arg 
        195                 200                 205             


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
    210                 215                 220                 


Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
225                 230                 235                 240 


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
                245                 250                 255     


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Gly 
            260                 265                 270         


Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser 
        275                 280                 285             


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
    290                 295                 300                 


Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
305                 310                 315                 320 


Pro Gln Arg Glu Ser Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile 
                325                 330                 335     


Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr 
            340                 345                 350         


Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys 
        355                 360                 365             


Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser 
    370                 375                 380                 


Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe 
385                 390                 395                 400 


Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp 
                405                 410                 415     


Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met 
            420                 425                 430         


Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu 
        435                 440                 445             


Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly 
    450                 455                 460                 


Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu 
465                 470                 475                 480 


Ser Ile Arg Asn Gly Thr Tyr Asn Tyr Pro Gln Tyr Ser Glu Glu Ala 
                485                 490                 495     


Arg Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly 
            500                 505                 510         


Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala 
        515                 520                 525             


Leu Ala Ile Met Met Ala Gly Leu Ser Thr Trp Met Cys Ser Asn Gly 
    530                 535                 540                 


Ser Leu Gln Cys Arg Ile Cys Ile Leu Val Pro Arg Gly Ser His His 
545                 550                 555                 560 


His His His His 
                


