                         SEQUENCE LISTING

<110>  Scil Proteins GmbH
 
<120>  Novel fusion proteins for capturing of cells

<130>  SP30 WO

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  76
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  2
<211>  152
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  bis-ubiquitin

<400>  2

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly Met Gln Ile Phe 
65                  70                  75                  80  


Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr Leu His 
    130                 135                 140                 


Leu Val Leu Arg Leu Arg Gly Gly 
145                 150         


<210>  3
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  reference ubiquitin 

<400>  3

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala 
65                  70                  75      


<210>  4
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein 148161 (control)

<400>  4

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala 
65                  70                  75                  80  


Ser Pro Ala Pro Ala Ala Glu Thr Val Arg Phe Gln Ser Ser Ala Pro 
                85                  90                  95      


Ala Pro Ser Ser Ala Pro Ala Ala Ser Ala Pro Pro Ala Ala Ala Ser 
            100                 105                 110         


Ala Cys 
        


<210>  5
<211>  82
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-139891

<400>  5

Met Gln Ile Phe Val Lys Thr Leu Thr Tyr Asn Pro Met Arg Tyr Gly 
1               5                   10                  15      


Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val 
            20                  25                  30          


Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg 
        35                  40                  45              


Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp 
    50                  55                  60                  


Tyr Asn Ile Arg Asn Pro Asp Ile Leu His Leu Val Leu Arg Leu Arg 
65                  70                  75                  80  


Ala Ala 
        


<210>  6
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein 144893

<400>  6

Met Gln Ile Phe Val Lys Thr Leu Thr Tyr Asn Pro Met Arg Tyr Gly 
1               5                   10                  15      


Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val 
            20                  25                  30          


Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg 
        35                  40                  45              


Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp 
    50                  55                  60                  


Tyr Asn Ile Arg Asn Pro Asp Ile Leu His Leu Val Leu Arg Leu Arg 
65                  70                  75                  80  


Ala Ala Ser Ala Pro Ala Ser Pro Ala Pro Ala Ala Glu Thr Val Arg 
                85                  90                  95      


Phe Gln Ser Ser Ala Pro Ala Pro Ser Ser Ala Pro Ala Ala Ser Ala 
            100                 105                 110         


Pro Pro Ala Ala Ala Ser Ala Cys 
        115                 120 


<210>  7
<211>  152
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-142628

<400>  7

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Thr Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Thr Leu Ala Phe Val Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu Trp Leu Tyr Leu Thr Trp Tyr Ala Ala Met Arg Ile Phe 
65                  70                  75                  80  


Val Thr Thr His Thr Gly Lys Thr Ile Thr Leu Asp Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Ser Glu Trp Ala Ile Leu His 
    130                 135                 140                 


Leu Val Leu Arg Leu Arg Ala Ala 
145                 150         


<210>  8
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein 148162

<400>  8

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Thr Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Thr Leu Ala Phe Val Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu Trp Leu Tyr Leu Thr Trp Tyr Ala Ala Met Arg Ile Phe 
65                  70                  75                  80  


Val Thr Thr His Thr Gly Lys Thr Ile Thr Leu Asp Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Ser Glu Trp Ala Ile Leu His 
    130                 135                 140                 


Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ser Pro Ala Pro 
145                 150                 155                 160 


Ala Ala Glu Thr Val Arg Phe Gln Ser Ser Ala Pro Ala Pro Ser Ser 
                165                 170                 175     


Ala Pro Ala Ala Ser Ala Pro Pro Ala Ala Ala Ser Ala Cys 
            180                 185                 190 


<210>  9
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein 144892

<400>  9

Met Gln Ile Phe Val Lys Thr Leu Thr Tyr Asn Pro Met Arg Tyr Gly 
1               5                   10                  15      


Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val 
            20                  25                  30          


Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg 
        35                  40                  45              


Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp 
    50                  55                  60                  


Tyr Asn Ile Arg Asn Pro Asp Ile Leu His Leu Val Leu Arg Leu Arg 
65                  70                  75                  80  


Ala Ala Ser Ala Pro Ala Ser Pro Ala Pro Ala Ala Glu Asn Leu Tyr 
                85                  90                  95      


Phe Gln Ser Ser Ala Pro Ala Pro Ser Ser Ala Pro Ala Ala Ser Ala 
            100                 105                 110         


Pro Pro Ala Ala Ala Ser Ala Cys 
        115                 120 


<210>  10
<211>  152
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-128187

<400>  10

Met Gln Ile Phe Val Pro Thr Asn Thr Gly Arg Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Ala Pro 
    50                  55                  60                  


Ser Gln Leu His Leu Val Arg Arg Leu Arg Ala Ala Met Gln Ile Phe 
65                  70                  75                  80  


Val Lys Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Trp Phe Tyr Tyr Ser Leu His 
    130                 135                 140                 


Leu Val Leu Arg Leu Arg Ala Ala 
145                 150         


<210>  11
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein 151318

<400>  11

Met Gln Ile Phe Val Pro Thr Asn Thr Gly Arg Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Ala Pro 
    50                  55                  60                  


Ser Gln Leu His Leu Val Arg Arg Leu Arg Ala Ala Met Gln Ile Phe 
65                  70                  75                  80  


Val Lys Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Trp Phe Tyr Tyr Ser Leu His 
    130                 135                 140                 


Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ser Pro Ala Pro 
145                 150                 155                 160 


Ala Ala Glu Thr Val Arg Phe Gln Ser Ser Ala Pro Ala Pro Ser Ser 
                165                 170                 175     


Ala Pro Ala Ala Ser Ala Pro Pro Ala Ala Ala Ser Ala Cys 
            180                 185                 190 


<210>  12
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker APS10

<400>  12

Ser Ala Pro Ala Ser Pro Ala Pro Ala Ala 
1               5                   10  


<210>  13
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker APS20

<400>  13

Ser Ala Pro Ala Pro Ser Ser Ala Pro Ala Ala Ser Ala Pro Pro Ala 
1               5                   10                  15      


Ala Ala Ser Ala 
            20  


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker APS15a

<400>  14

Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala 
1               5                   10                  15  


<210>  15
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker APS15b

<400>  15

Ala Ser Pro Ala Pro Ala Ala Ala Pro Ser Ala Pro Ala Pro Ala 
1               5                   10                  15  


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker SG

<400>  16

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10  


<210>  17
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker GSG

<400>  17

Gly Ser Gly Gly Gly Gly 
1               5       


<210>  18
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker G4S3

<400>  18

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  19
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker G4S

<400>  19

Gly Gly Gly Gly Ser 
1               5   


<210>  20
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker SG4

<400>  20

Ser Gly Gly Gly Gly 
1               5   


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PCS TVMV 1

<400>  21

Glu Thr Val Arg Phe Gln Ser 
1               5           


<210>  22
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PCS TVMV 2

<400>  22

Glu Thr Val Arg Phe Gln Gly 
1               5           


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PCS TEV 1

<400>  23

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ENLYFQS

<400>  24

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  25
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  StrepTag

<400>  25

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


