﻿               SEQUENCE LISTING

<110> Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

<120> Means and methods for inhibiting gene expression

<130> Y2237 PCT

<150> EP16155719.4
<151> 2016-02-15

<160> 11

<170> BiSSAP 1.3

<210> 1
<211> 80
<212> PRT
<213> Artificial Sequence

<220> 
<223> THOV-ML AMINO ACIDS 225-304

<400> 1
Leu Ile Gly Trp Met Asp Glu Ile Ile Lys Ile Phe Ser Lys Pro Asp 
1               5                   10                  15      
Tyr Leu Glu Ala Lys Gly Val Ser Tyr Gln Val Leu Lys Asn Val Ser 
            20                  25                  30          
Asn Lys Val Ala Leu Leu Arg Glu Ser Ile Trp Trp Val Thr Glu Leu 
        35                  40                  45              
Asp Gly Arg Glu Tyr Leu Phe Phe Asp Glu Ser Trp Tyr Leu His Gly 
    50                  55                  60                  
Met Ser Ala Phe Ser Asp Gly Val Pro Gly Tyr Glu Asp Phe Ile Tyr 
65                  70                  75                  80  



<210> 2
<211> 70
<212> PRT
<213> Artificial Sequence

<220> 
<223> THOV-ML AMINO ACIDS 235-304

<400> 2
Ile Phe Ser Lys Pro Asp Tyr Leu Glu Ala Lys Gly Val Ser Tyr Gln 
1               5                   10                  15      
Val Leu Lys Asn Val Ser Asn Lys Val Ala Leu Leu Arg Glu Ser Ile 
            20                  25                  30          
Trp Trp Val Thr Glu Leu Asp Gly Arg Glu Tyr Leu Phe Phe Asp Glu 
        35                  40                  45              
Ser Trp Tyr Leu His Gly Met Ser Ala Phe Ser Asp Gly Val Pro Gly 
    50                  55                  60                  
Tyr Glu Asp Phe Ile Tyr 
65                  70  

<210> 3
<211> 60
<212> PRT
<213> Artificial Sequence

<220> 
<223> THOV-ML AMINO ACIDS 245-304

<400> 3
Lys Gly Val Ser Tyr Gln Val Leu Lys Asn Val Ser Asn Lys Val Ala 
1               5                   10                  15      
Leu Leu Arg Glu Ser Ile Trp Trp Val Thr Glu Leu Asp Gly Arg Glu 
            20                  25                  30          
Tyr Leu Phe Phe Asp Glu Ser Trp Tyr Leu His Gly Met Ser Ala Phe 
        35                  40                  45              
Ser Asp Gly Val Pro Gly Tyr Glu Asp Phe Ile Tyr 
    50                  55                  60  

<210> 4
<211> 58
<212> PRT
<213> Artificial Sequence

<220> 
<223> THOV-ML AMINO ACIDS 247-304

<400> 4
Val Ser Tyr Gln Val Leu Lys Asn Val Ser Asn Lys Val Ala Leu Leu 
1               5                   10                  15      
Arg Glu Ser Ile Trp Trp Val Thr Glu Leu Asp Gly Arg Glu Tyr Leu 
            20                  25                  30          
Phe Phe Asp Glu Ser Trp Tyr Leu His Gly Met Ser Ala Phe Ser Asp 
        35                  40                  45              
Gly Val Pro Gly Tyr Glu Asp Phe Ile Tyr 
    50                  55              

<210> 5
<211> 57
<212> PRT
<213> Artificial Sequence

<220> 
<223> THOV-ML AMINO ACIDS 248-304

<400> 5
Ser Tyr Gln Val Leu Lys Asn Val Ser Asn Lys Val Ala Leu Leu Arg 
1               5                   10                  15      
Glu Ser Ile Trp Trp Val Thr Glu Leu Asp Gly Arg Glu Tyr Leu Phe 
            20                  25                  30          
Phe Asp Glu Ser Trp Tyr Leu His Gly Met Ser Ala Phe Ser Asp Gly 
        35                  40                  45              
Val Pro Gly Tyr Glu Asp Phe Ile Tyr 
    50                  55          

<210> 6
<211> 56
<212> PRT
<213> Artificial Sequence

<220> 
<223> THOV-ML AMINO ACIDS 249-304

<400> 6
Tyr Gln Val Leu Lys Asn Val Ser Asn Lys Val Ala Leu Leu Arg Glu 
1               5                   10                  15      
Ser Ile Trp Trp Val Thr Glu Leu Asp Gly Arg Glu Tyr Leu Phe Phe 
            20                  25                  30          
Asp Glu Ser Trp Tyr Leu His Gly Met Ser Ala Phe Ser Asp Gly Val 
        35                  40                  45              
Pro Gly Tyr Glu Asp Phe Ile Tyr 
    50                  55      

<210> 7
<211> 58
<212> PRT
<213> Artificial Sequence

<220> 
<223> THOV-ML AMINO ACIDS 247-304 (not binding SOLH)

<400> 7
Val Ser Tyr Gln Val Leu Lys Asn Val Ser Asn Lys Val Ala Leu Leu 
1               5                   10                  15      
Arg Glu Ser Ile Trp Trp Val Ala Ala Leu Asp Gly Arg Glu Tyr Leu 
            20                  25                  30          
Phe Phe Asp Glu Ser Trp Tyr Leu His Gly Met Ser Ala Phe Ser Asp 
        35                  40                  45              
Gly Val Pro Gly Tyr Glu Asp Phe Ile Tyr 
    50                  55              

<210> 8
<211> 57
<212> PRT
<213> Artificial Sequence

<220> 
<223> THOV-ML AMINO ACIDS 248-304 (not binding SOLH)

<400> 8
Ser Tyr Gln Val Leu Lys Asn Val Ser Asn Lys Val Ala Leu Leu Arg 
1               5                   10                  15      
Glu Ser Ile Trp Trp Val Ala Ala Leu Asp Gly Arg Glu Tyr Leu Phe 
            20                  25                  30          
Phe Asp Glu Ser Trp Tyr Leu His Gly Met Ser Ala Phe Ser Asp Gly 
        35                  40                  45              
Val Pro Gly Tyr Glu Asp Phe Ile Tyr 
    50                  55          

<210> 9
<211> 56
<212> PRT
<213> Artificial Sequence

<220> 
<223> THOV-ML AMINO ACIDS 249-304 (not binding SOLH)

<400> 9
Tyr Gln Val Leu Lys Asn Val Ser Asn Lys Val Ala Leu Leu Arg Glu 
1               5                   10                  15      
Ser Ile Trp Trp Val Ala Ala Leu Asp Gly Arg Glu Tyr Leu Phe Phe 
            20                  25                  30          
Asp Glu Ser Trp Tyr Leu His Gly Met Ser Ala Phe Ser Asp Gly Val 
        35                  40                  45              
Pro Gly Tyr Glu Asp Phe Ile Tyr 
    50                  55      

<210> 10
<211> 304
<212> PRT
<213> Thogoto virus

<220> 
<223> ML amino acid sequence

<400> 10
Met Ala Thr Ser Leu Pro Val Arg Ala Phe Ser Glu Val Cys Cys Gln 
1               5                   10                  15      
Glu Ala Lys Ala Ala Ile Ile Gln Met Glu Asn Asn Pro Asp Glu Thr 
            20                  25                  30          
Val Cys Asn Arg Ile Trp Lys Ile His Arg Asp Met Gln Ser Ser Asp 
        35                  40                  45              
Leu Thr Thr Thr Val Gln Val Met Met Val Tyr Arg Phe Ile Ser Lys 
    50                  55                  60                  
Arg Val Pro Glu Gly Cys Phe Thr Ile Leu Ser Gly Ile Asn Thr Ser 
65                  70                  75                  80  
Leu Tyr Asn Pro Arg Glu Leu Lys Arg Ser Tyr Val Gln Ala Ile Ser 
                85                  90                  95      
Thr Gly Thr Ser Cys Glu Phe Leu Lys Ser Leu Asp Lys Leu Ala Arg 
            100                 105                 110         
Asn Leu Leu Ala Val His Val Cys Ser Asp Val Lys Met Ser Leu Ser 
        115                 120                 125             
Lys Arg Gln Val Ile Asp Phe Leu Ser Gly Glu Asn Asp Pro Ala Leu 
    130                 135                 140                 
His Ala Ala Glu His Leu Thr Ser Leu Ala Leu Asp Asp Ser Pro Ser 
145                 150                 155                 160 
Ala Val Met Tyr Ser Gly Trp Gln Gln Glu Ala Val Lys Leu His Asn 
                165                 170                 175     
Thr Ile Arg Lys Ile Ala Thr Met Arg Pro Ala Asp Cys Lys Ala Gly 
            180                 185                 190         
Lys Phe Tyr Ser Asp Ile Leu Ser Ala Cys Asp Gln Thr Lys Glu Leu 
        195                 200                 205             
Leu Asp Ser Phe Asp Gln Gly Lys Leu Ser Tyr Asp Arg Asp Val Val 
    210                 215                 220                 
Leu Ile Gly Trp Met Asp Glu Ile Ile Lys Ile Phe Ser Lys Pro Asp 
225                 230                 235                 240 
Tyr Leu Glu Ala Lys Gly Val Ser Tyr Gln Val Leu Lys Asn Val Ser 
                245                 250                 255     
Asn Lys Val Ala Leu Leu Arg Glu Ser Ile Trp Trp Val Thr Glu Leu 
            260                 265                 270         
Asp Gly Asn Glu Tyr Leu Phe Phe Asp Asp Ser Trp Tyr Leu His Gly 
        275                 280                 285             
Ile Ser Ala Phe Ser Asp Gly Val Pro Gly Tyr Glu Asp Phe Ile His 
    290                 295                 300                 



<210> 11
<211> 915
<212> DNA
<213> Thogoto virus

<220> 
<223> ML complete cDNA sequence

<400> 11
atggcttcca acctaccagt gaggtctttc agcgaagtct gctgtgctga ggcacgggcg      60

gctataatac agatggagaa caaccccgac gaaaccgtgt gtaataggat ctggaagatc     120

caccgggacc tacagtcaag tgacctcacc accactgtgc aagtaatgat ggtgtatcgt     180

ttcatatcaa aacgggtgcc agaaggctgc ttcgccatcc tttctggtgt gaacactgga     240

atgtacaacc caagagagct taaacgaagt tatgtgcaat ccctgtcttc aggaacctcc     300

tgtgagtttc tgagatcatt agacaagcta gcaaagaact tgctggctgt acatgtctgc     360

tctgatgtga agatgtccct caacaaaagg caggtgatag attttataag cggtgaagaa     420

gatcccaccc tccacacagc agagcatcta acaagcttgg ccctggatga cagcccttct     480

gcagtggtgt acagtggatg gcagcaggaa gcgatcaagc ttcacaacac catcaggaaa     540

attgctacga tgaggcctgc agactgcaaa gcagggaagt tctactcgga catcttatcc     600

gcctgcgacc agacaaaaga acttctagac gccttcgacc agggaaaact tgcctatgac     660

cgagacgtag tgctcatcgg gtggatggat gagatcatca aaatcttctc aaaacctgat     720

tatctggagg cgaagggagt ctcatatcag gtcctcaaga acgtcagcaa caaagtcgcc     780

cttctccggg aaagcatttg gtgggttaca gaattagatg gaagagaata tctcttcttc     840

gatgagtctt ggtacttaca tggcatgtct gctttttcag atggtgtgcc tggatacgaa     900

gacttcattt actaa                                                      915


