                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
       Greene, Mark I
       Zhang, Hongtao
       Tsuchiya, Hiromichi
       Nagai, Yasuhiro
       Lam, Lian
       Runkle, Aaron
       Drebin, Jeffrey
       Ji, Mei Qing
 
<120>  COMBINATION OF IFN-GAMMA WITH ERBB KINASE INHIBITOR FOR THE 
       TREATMENT OF CANCERS

<130>  PENN-00203WO_321199_TBA

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  324
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4D5scFvZZ

<400>  1

Met Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
1               5                   10                  15      


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn 
            20                  25                  30          


Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
        35                  40                  45              


Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe 
    50                  55                  60                  


Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
65                  70                  75                  80  


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr 
                85                  90                  95      


Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Leu Lys Arg Ala Thr 
            100                 105                 110         


Pro Ser His Asn Ser His Gln Val Pro Ser Ala Gly Gly Pro Thr Ala 
        115                 120                 125             


Asn Ser Gly Glu Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
    130                 135                 140                 


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Phe Asn Ile 
145                 150                 155                 160 


Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
                165                 170                 175     


Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala 
            180                 185                 190         


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
        195                 200                 205             


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
    210                 215                 220                 


Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr 
225                 230                 235                 240 


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
                245                 250                 255     


Val Asp Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr 
            260                 265                 270         


Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe 
        275                 280                 285             


Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala 
    290                 295                 300                 


Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Leu Glu His His 
305                 310                 315                 320 


His His His His 
                


<210>  2
<211>  464
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4D5scFvZZ-IFN-gamma

<400>  2

Met Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
1               5                   10                  15      


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn 
            20                  25                  30          


Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
        35                  40                  45              


Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe 
    50                  55                  60                  


Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
65                  70                  75                  80  


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr 
                85                  90                  95      


Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Leu Lys Arg Ala Thr 
            100                 105                 110         


Pro Ser His Asn Ser His Gln Val Pro Ser Ala Gly Gly Pro Thr Ala 
        115                 120                 125             


Asn Ser Gly Glu Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
    130                 135                 140                 


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Phe Asn Ile 
145                 150                 155                 160 


Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
                165                 170                 175     


Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala 
            180                 185                 190         


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
        195                 200                 205             


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
    210                 215                 220                 


Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr 
225                 230                 235                 240 


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
                245                 250                 255     


Val Asp Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr 
            260                 265                 270         


Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe 
        275                 280                 285             


Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala 
    290                 295                 300                 


Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Leu Glu Gly Gly 
305                 310                 315                 320 


Gly Gly Ser Gln Asp Pro Tyr Val Lys Glu Ala Glu Asn Leu Lys Lys 
                325                 330                 335     


Tyr Phe Asn Ala Gly His Ser Asp Val Ala Asp Asn Gly Thr Leu Phe 
            340                 345                 350         


Leu Gly Ile Leu Lys Asn Trp Lys Glu Glu Ser Asp Arg Lys Ile Met 
        355                 360                 365             


Gln Ser Gln Ile Val Ser Phe Tyr Phe Lys Leu Phe Lys Asn Phe Lys 
    370                 375                 380                 


Asp Asp Gln Ser Ile Gln Lys Ser Val Glu Thr Ile Lys Glu Asp Met 
385                 390                 395                 400 


Asn Val Lys Phe Phe Asn Ser Asn Lys Lys Lys Arg Asp Asp Phe Glu 
                405                 410                 415     


Lys Leu Thr Asn Tyr Ser Val Thr Asp Leu Asn Val Gln Arg Lys Ala 
            420                 425                 430         


Ile His Glu Leu Ile Gln Val Met Ala Glu Leu Ser Pro Ala Ala Lys 
        435                 440                 445             


Thr Gly Lys Arg Lys Arg Ser Gln Leu Glu His His His His His His 
    450                 455                 460                 


<210>  3
<211>  259
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4D5scFv

<400>  3

Met Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
1               5                   10                  15      


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn 
            20                  25                  30          


Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
        35                  40                  45              


Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe 
    50                  55                  60                  


Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
65                  70                  75                  80  


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr 
                85                  90                  95      


Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Leu Lys Arg Ala Thr 
            100                 105                 110         


Pro Ser His Asn Ser His Gln Val Pro Ser Ala Gly Gly Pro Thr Ala 
        115                 120                 125             


Asn Ser Gly Glu Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
    130                 135                 140                 


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Phe Asn Ile 
145                 150                 155                 160 


Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
                165                 170                 175     


Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala 
            180                 185                 190         


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
        195                 200                 205             


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
    210                 215                 220                 


Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr 
225                 230                 235                 240 


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Leu Glu His His His 
                245                 250                 255     


His His His 
            


<210>  4
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZZ

<400>  4

Ser Val Asp Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe 
1               5                   10                  15      


Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala 
            20                  25                  30          


Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu 
        35                  40                  45              


Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55                  60      


<210>  5
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IFN-gamma

<400>  5

Gln Asp Pro Tyr Val Lys Glu Ala Glu Asn Leu Lys Lys Tyr Phe Asn 
1               5                   10                  15      


Ala Gly His Ser Asp Val Ala Asp Asn Gly Thr Leu Phe Leu Gly Ile 
            20                  25                  30          


Leu Lys Asn Trp Lys Glu Glu Ser Asp Arg Lys Ile Met Gln Ser Gln 
        35                  40                  45              


Ile Val Ser Phe Tyr Phe Lys Leu Phe Lys Asn Phe Lys Asp Asp Gln 
    50                  55                  60                  


Ser Ile Gln Lys Ser Val Glu Thr Ile Lys Glu Asp Met Asn Val Lys 
65                  70                  75                  80  


Phe Phe Asn Ser Asn Lys Lys Lys Arg Asp Asp Phe Glu Lys Leu Thr 
                85                  90                  95      


Asn Tyr Ser Val Thr Asp Leu Asn Val Gln Arg Lys Ala Ile His Glu 
            100                 105                 110         


Leu Ile Gln Val Met Ala Glu Leu Ser Pro Ala Ala Lys Thr Gly Lys 
        115                 120                 125             


Arg Lys Arg Ser Gln 
    130             


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glycine-serine oligopeptide linker

<400>  6

Gly Gly Gly Gly Ser 
1               5   


<210>  7
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glycine-serine oligopeptide linker

<400>  7

Gly Gly Gly Gly Gly Ser 
1               5       


<210>  8
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  snail 6SA insert forward primer

<400>  8
aaagaagctt atgccgcgct ctttcctc                                          28


<210>  9
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  snail 6SA insert reverse primer

<400>  9
aaagtctaga tcagcgggga catcctgagc ag                                     32


