                         SEQUENCE LISTING

<110>  RGENE, INC.
 
<120>  MULTIPLE LIGASE COMPOSITIONS, SYSTEMS, AND METHODS

<130>  RGENE-34712/WO-1/ORD

<150>  US 62/292,558
<151>  2016-02-08

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  1

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Ser Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu 
        355                 360                 365             


Val Ile Asp Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  2
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  2

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Cys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu 
        355                 360                 365             


Val Ile Asp Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  3
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  3

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Ala Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu 
        355                 360                 365             


Val Ile Asp Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  4
<211>  64
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  4
gatcggaaga gcacacgtct gaactccagt cuacactctt tccctacacg acgctcttcc       60

gatc                                                                    64


<210>  5
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa= D or N

<400>  5

Gly Xaa Xaa Gly 
1               


<210>  6
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  6

Pro Xaa Xaa Gly 
1               


<210>  7
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  7

Ala Xaa Xaa Gly 
1               


<210>  8
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  8

Val Xaa Xaa Gly 
1               


<210>  9
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  9

Leu Xaa Xaa Gly 
1               


<210>  10
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  10

Ile Xaa Xaa Gly 
1               


<210>  11
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  11

Met Xaa Xaa Gly 
1               


<210>  12
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  12

Cys Xaa Xaa Gly 
1               


<210>  13
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  13

Phe Xaa Xaa Gly 
1               


<210>  14
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  14

Tyr Xaa Xaa Gly 
1               


<210>  15
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  15

Trp Xaa Xaa Gly 
1               


<210>  16
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  16

His Xaa Xaa Gly 
1               


<210>  17
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  17

Arg Xaa Xaa Gly 
1               


<210>  18
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  18

Gln Xaa Xaa Gly 
1               


<210>  19
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  19

Asn Xaa Xaa Gly 
1               


<210>  20
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  20

Glu Xaa Xaa Gly 
1               


<210>  21
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  21

Asp Xaa Xaa Gly 
1               


<210>  22
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  22

Ser Xaa Xaa Gly 
1               


<210>  23
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa=D or N

<400>  23

Thr Xaa Xaa Gly 
1               


<210>  24
<211>  3
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa=any amino acid

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa=D or N

<400>  24

Xaa Xaa Gly 
1           


