                         SEQUENCE LISTING

<110>  The University Court of the University of Edinburgh
 
<120>  Improved Flu Vaccine Yield

<130>  P230814WO

<150>  1602535.5
<151>  2016-02-12

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  2341
<212>  DNA
<213>  Influenza A virus

<400>  1
agcgaaagca ggtcaattat attcaatatg gaaagaataa aagaactaag aaatctaatg       60

tcgcagtctc gcacccgcga gatactcaca aaaaccaccg tggaccatat ggccataatc      120

aagaagtaca catcaggaag acaggagaag aacccagcac ttaggatgaa atggatgatg      180

gcaatgaaat atccaattac agcagacaag aggataacgg aaatgattcc tgagagaaat      240

gagcaaggac aaactttatg gagtaaaatg aatgatgccg gatcagaccg agtgatggta      300

tcaccactgg ctgtgacatg gtggaatagg aatggaccaa taacaaatac agttcattat      360

ccaaaaatct acaaaactta ttttgaaaga gtcgaaaggc taaagcatgg aacctttggc      420

cctgtccatt ttagaaacca agtcaaaata cgtcggagag ttgacataaa tcctggtcat      480

gcagatctca gtgccaagga ggcacaggat gtaatcatgg aagttgtttt ccctaacgaa      540

gtgggagcca ggatactaac atcggaatcg caactaacga taaccaaaga gaagaaagaa      600

gaactccagg attgcaaaat ttctcctttg atggttgcat acatgttgga gagagaactg      660

gtccgcaaaa cgagattcct cccagtggct ggtggaacaa gcagtgtgta cattgaagtg      720

ttgcatttga ctcaaggaac atgctgggaa cagatgtata ctccaggagg ggaagtgagg      780

aatgatgatg ttgatcaaag cttgattatt gctgctagga acatagtgag aagagctgca      840

gtatcagcag atccactagc atctttattg gagatgtgcc acagcacaca gattggtgga      900

attaggatgg tagacatcct taggcagaac ccaacagaag agcaagccgt ggatatatgc      960

aaggctgcaa tgggactgag aattagctca tccttcagtt ttggtggatt cacatttaag     1020

agaacaagcg gatcatcagt caagagagag gaagaggtgc ttacgggcaa tcttcaaaca     1080

ttgaagataa gagtgcatga gggatatgaa gagttcacaa tggttgggag aagagcaaca     1140

gccatactca gaaaagcaac caggagattg attcagctga tagtgagtgg gagagacgaa     1200

cagtcgattg ccgaagcaat aattgtggcc atggtatttt cacaagagga ttgtatgata     1260

aaagcagtca gaggtgatct gaatttcgtc aatagggcga atcagcgatt gaatcctatg     1320

catcaacttt taagacattt tcagaaggat gcgaaagtgc tttttcaaaa ttggggagtt     1380

gaacctatcg acaatgtgat gggaatgatt gggatattgc ccgacatgac tccaagcatc     1440

gagatgtcaa tgagaggagt gagaatcagc aaaatgggtg tagatgagta ctccagcacg     1500

gagagggtag tggtgagcat tgaccgtttt ttgagaatcc gggaccaacg aggaaatgta     1560

ctactgtctc ccgaggaggt cagtgaaaca cagggaacag agaaactgac aataacttac     1620

tcatcgtcaa tgatgtggga gattaatggt cctgaatcag tgttggtcaa tacctatcaa     1680

tggatcatca gaaactggga aactgttaaa attcagtggt cccagaaccc tacaatgcta     1740

tacaataaaa tggaatttga accatttcag tctttagtac ctaaggccat tagaggccaa     1800

tacagtgggt ttgtaagaac tctgttccaa caaatgaggg atgtgcttgg gacatttgat     1860

accgcacaga taataaaact tcttcccttc gcagccgctc caccaaagca aagtagaatg     1920

cagttctcct catttactgt gaatgtgagg ggatcaggaa tgagaatact tgtaaggggc     1980

aattctcctg tattcaacta taacaaggcc acgaagagac tcacagttct cggaaaggat     2040

gctggcactt taactgaaga cccagatgaa ggcacagctg gagtggagtc cgctgttctg     2100

aggggattcc tcattctggg caaagagaac aagagatatg ggccagcact aagcatcaat     2160

gaactgagca accttgcgaa aggagagaag gctaatgtgc taattgggca aggagacgtg     2220

gtgttggtaa tgaaacggaa acgggactct agcatactta ctgacagcca gacagcgacc     2280

aaaagaattc ggatggccat caattagtgt cgaatagttt aaaaacgacc ttgtttctac     2340

t                                                                     2341


<210>  2
<211>  2341
<212>  DNA
<213>  Influenza A virus

<400>  2
agcgaaagca ggcaaaccat ttgaatggat gtcaatccga ccttactttt cttaaaagtg       60

ccagcacaaa atgctataag cacaactttc ccttatactg gagaccctcc ttacagccat      120

gggacaggaa caggatacac catggatact gtcaacagga cacatcagta ctcagaaaag      180

ggaagatgga caacaaacac cgaaactgga gcaccgcaac tcaacccgat tgatgggcca      240

ctgccagaag acaatgaacc aagtggttat gcccaaacag attgtgtatt ggaggcgatg      300

gctttccttg aggaatccca tcctggtatt tttgaaaact cgtgtattga aacgatggag      360

gttgttcagc aaacacgagt agacaagctg acacaaggcc gacagaccta tgactggact      420

ctaaatagaa accaacctgc tgcaacagca ttggccaaca caatagaagt gttcagatca      480

aatggcctca cggccaatga gtctggaagg ctcatagact tccttaagga tgtaatggag      540

tcaatgaaca aagaagaaat ggggatcaca actcattttc agagaaagag acgggtgaga      600

gacaatatga ctaagaaaat gataacacag agaacaatgg gtaaaaagaa gcagagattg      660

aacaaaagga gttatctaat tagagcattg accctgaaca caatgaccaa agatgctgag      720

agagggaagc taaaacggag agcaattgca accccaggga tgcaaataag ggggtttgta      780

tactttgttg agacactggc aaggagtata tgtgagaaac ttgaacaatc agggttgcca      840

gttggaggca atgagaagaa agcaaagttg gcaaatgttg taaggaagat gatgaccaat      900

tctcaggaca ccgaactttc tttcaccatc actggagata acaccaaatg gaacgaaaat      960

cagaatcctc ggatgttttt ggccatgatc acatatatga ccagaaatca gcccgaatgg     1020

ttcagaaatg ttctaagtat tgctccaata atgttctcaa acaaaatggc gagactggga     1080

aaagggtata tgtttgagag caagagtatg aaacttagaa ctcaaatacc tgcagaaatg     1140

ctagcaagca tcgatttgaa atatttcaat gattcaacaa gaaagaagat tgaaaaaatc     1200

cgatcgctct taatagaggg gactgcatca ttgagccctg gaatgatgat gggcatgttc     1260

aatatgttaa gcactgtatt aggcgtctcc atcctgaatc ttggacaaaa gagatacacc     1320

aagactactt actggtggga tggtcttcaa tcctctgacg attttgctct gattgtgaat     1380

gcacccaatc atgaagggat tcaagccgga gtcgacaggt tttatcgaac ctgtaagcta     1440

cttggaatca atatgagcaa gaaaaagtct tacataaaca gaacaggtac atttgaattc     1500

acaagttttt tctatcgtta tgggtttgtt gccaatttca gcatggagct tcccagtttt     1560

ggggtgtctg ggatcaacga gtcagcggac atgagtattg gagttactgt catcaaaaac     1620

aatatgataa acaatgatct tggtccagca acagctcaaa tggcccttca gttgttcatc     1680

aaagattaca ggtacacgta ccgatgccat agaggtgaca cacaaataca aacccgaaga     1740

tcatttgaaa taaagaaact gtgggagcaa acccgttcca aagctggact gctggtctcc     1800

gacggaggcc caaatttata caacattaga aatctccaca ttcctgaagt ctgcctaaaa     1860

tgggaattga tggatgagga ttaccagggg cgtttatgca acccactgaa cccatttgtc     1920

agccataaag aaattgaatc aatgaacaat gcagtgatga tgccagcaca tggtccagcc     1980

aaaaacatgg agtatgatgc tgttgcaaca acacactcct ggatccccaa aagaaatcga     2040

tccatcttga atacaagtca aagaggagta cttgaggatg aacaaatgta ccaaaggtgc     2100

tgcaatttat ttgaaaaatt cttccccagc agttcataca gaagaccagt cgggatatcc     2160

agtatggtgg aggctatggt ttccagagcc cgaattgatg cacggattga tttcgaatct     2220

ggaaggataa agaaagaaga gttcactgag atcatgaaga tctgttccac cattgaagag     2280

ctcagacggc aaaaatagtg aatttagctt gtccttcatg aaaaaatgcc ttgtttctac     2340

t                                                                     2341


<210>  3
<211>  2233
<212>  DNA
<213>  Influenza A virus

<400>  3
agcgaaagca ggtactgatc caaaatggaa gattttgtgc gacaatgctt caatccgatg       60

attgtcgagc ttgcggaaaa aacaatgaaa gagtatgggg aggacctgaa aatcgaaaca      120

aacaaatttg cagcaatatg cactcacttg gaagtatgct tcatgtattc agattttcac      180

ttcatcaatg agcaaggcga gtcaataatc gtagaacttg gtgatccaaa tgcacttttg      240

aagcacagat ttgaaataat cgagggaaga gatcgcacaa tggcctggac agtagtaaac      300

agtatttgca acactacagg ggctgagaaa ccaaagtttc taccagattt gtatgattac      360

aaggagaata gattcatcga aattggagta acaaggagag aagttcacat atactatctg      420

gaaaaggcca ataaaattaa atctgagaaa acacacatcc acattttctc gttcactggg      480

gaagaaatgg ccacaaaggc agactacact ctcgatgaag aaagcagggc taggatcaaa      540

accagactat tcaccataag acaagaaatg gccagcagag gcctctggga ttcctttcgt      600

cagtccgaga gaggagaaga gacaattgaa gaaaggtttg aaatcacagg aacaatgcgc      660

aagcttgccg accaaagtct cccgccgaac ttctccagcc ttgaaaattt tagagcctat      720

gtggatggat tcgaaccgaa cggctacatt gagggcaagc tgtctcaaat gtccaaagaa      780

gtaaatgcta gaattgaacc ttttttgaaa acaacaccac gaccacttag acttccgaat      840

gggcctccct gttctcagcg gtccaaattc ctactgatgg atgccttaaa attaagcatt      900

gaggacccaa gtcatgaagg agagggaata ccgctatatg atgcaatcaa atgcatgaga      960

acattctttg gatggaagga acccaatgtt gttaaaccac acgaaaaggg aataaatcca     1020

aattatcttc tgtcatggaa gcaagtactg gcagaactgc aggacattga gaatgaggag     1080

aaaattccaa agactaaaaa tatgaagaaa acaagtcagc taaagtgggc acttggtgag     1140

aacatggcac cagaaaaggt agactttgac gactgtaaag atgtaggtga tttgaagcaa     1200

tatgatagtg atgaaccaga attgaggtcg cttgcaagtt ggattcagaa tgagtttaac     1260

aaggcatgcg aactgacaga ttcaagctgg atagagctcg atgagattgg agaagatgtg     1320

gctccaattg aacacattgc aagcatgaga aggaattatt tcacatcaga ggtgtctcac     1380

tgcagagcca cagaatacat aatgaagggg gtgtacatca atactgcctt gcttaatgca     1440

tcttgtgcag caatggatga tttccaatta attccaatga taagcaagtg tagaactaag     1500

gagggaaggc gaaagaccaa cttgtatggt ttcatcataa aaggaagatc ccacttaagg     1560

aatgacaccg acgtggtaaa ctttgtgagc atggagtttt ctctcactga cccaagactt     1620

gaaccacata aatgggagaa gtactgtgtt cttgagatag gagatatgct tataagaagt     1680

gccataggcc aggtttcaag gcccatgttc ttgtatgtga gaacaaatgg aacctcaaaa     1740

attaaaatga aatggggaat ggagatgagg cgttgcctcc tccagtcact tcaacaaatt     1800

gagagtatga ttgaagctga gtcctctgtc aaagagaaag acatgaccaa agagttcttt     1860

gagaacaaat cagaaacatg gcccattgga gagtccccca aaggagtgga ggaaagttcc     1920

attgggaagg tctgcaggac tttattagca aagtcggtat tcaacagctt gtatgcatct     1980

ccacaactag aaggattttc agctgaatca agaaaactgc ttcttatcgt tcaggctctt     2040

agggacaacc ttgaacctgg gacctttgat cttggggggc tatatgaagc aattgaggag     2100

tgcctgatta atgatccctg ggttttgctt aatgcttctt ggttcaactc attccttaca     2160

catgcattga gttagttgtg gcagtgctac tatttgctat ccatactgtc caaaaaagta     2220

ccttgtttct act                                                        2233


<210>  4
<211>  1565
<212>  DNA
<213>  Influenza A virus

<400>  4
agcaaaagca gggtagataa tcactcactg agtgacatca aaatcatggc gtctcaaggc       60

accaaacgat cttacgaaca gatggagact gatggagaac gccagaatgc cactgaaatc      120

agagcatccg tcggaaaaat gattggtgga attggacgat tctacatcca aatgtgcacc      180

gaactcaaac tcagtgatta tgagggacgg ttgatccaaa acagcttaac aatagagaga      240

atggtgctct ctgcttttga cgaaaggaga aataaatacc ttgaagaaca tcccagtgcg      300

gggaaagatc ctaagaaaac tggaggacct atatacagga gagtaaacgg aaagtggatg      360

agagaactca tcctttatga caaagaagaa ataaggcgaa tctggcgcca agctaataat      420

ggtgacgatg caacggctgg tctgactcac atgatgatct ggcattccaa tttgaatgat      480

gcaacttatc agaggacaag agctcttgtt cgcaccggaa tggatcccag gatgtgctct      540

ctgatgcaag gttcaactct ccctaggagg tctggagccg caggtgctgc agtcaaagga      600

gttggaacaa tggtgatgga attggtcaga atgatcaaac gtgggatcaa tgatcggaac      660

ttctggaggg gtgagaatgg acgaaaaaca agaattgctt atgaaagaat gtgcaacatt      720

ctcaaaggga aatttcaaac tgctgcacaa aaagcaatga tggatcaagt gagagagagc      780

cggaacccag ggaatgctga gttcgaagat ctcacttttc tagcacggtc tgcactcata      840

ttgagagggt cggttgctca caagtcctgc ctgcctgcct gtgtgtatgg acctgccgta      900

gccagtgggt acgactttga aagggaggga tactctctag tcggaataga ccctttcaga      960

ctgcttcaaa acagccaagt gtacagccta atcagaccaa atgagaatcc agcacacaag     1020

agtcaactgg tgtggatggc atgccattct gccgcatttg aagatctaag agtattaagc     1080

ttcatcaaag ggacgaaggt gctcccaaga gggaagcttt ccactagagg agttcaaatt     1140

gcttccaatg aaaatatgga gactatggaa tcaagtacac ttgaactgag aagcaggtac     1200

tgggccataa ggaccagaag tggaggaaac accaatcaac agagggcatc tgcgggccaa     1260

atcagcatac aacctacgtt ctcagtacag agaaatctcc cttttgacag aacaaccatt     1320

atggcagcat tcaatgggaa tacagagggg agaacatctg acatgaggac cgaaatcata     1380

aggatgatgg aaagtgcaag accagaagat gtgtctttcc aggggcgggg agtcttcgag     1440

ctctcggacg aaaaggcagc gagcccgatc gtgccttcct ttgacatgag taatgaagga     1500

tcttatttct tcggagacaa tgcagaggag tacgacaatt aaagaaaaat acccttgttt     1560

ctact                                                                 1565


<210>  5
<211>  1027
<212>  DNA
<213>  Influenza A virus

<400>  5
agcgaaagca ggtagatatt gaaagatgag tcttctaacc gaggtcgaaa cgtacgtact       60

ctctatcatc ccgtcaggcc ccctcaaagc cgagatcgca cagagacttg aagatgtctt      120

tgcagggaag aacaccgatc ttgaggttct catggaatgg ctaaagacaa gaccaatcct      180

gtcacctctg actaagggga ttttaggatt tgtgttcacg ctcaccgtgc ccagtgagcg      240

aggactgcag cgtagacgct ttgtccaaaa tgcccttaat gggaacgggg atccaaataa      300

catggacaaa gcagttaaac tgtataggaa gctcaagagg gagataacat tccatggggc      360

caaagaaatc tcactcagtt attctgctgg tgcacttgcc agttgtatgg gcctcatata      420

caacaggatg ggggctgtga ccactgaagt ggcatttggc ctggtatgtg caacctgtga      480

acagattgct gactcccagc atcggtctca taggcaaatg gtgacaacaa ccaatccact      540

aatcagacat gagaacagaa tggttttagc cagcactaca gctaaggcta tggagcaaat      600

ggctggatcg agtgagcaag cagcagaggc catggaggtt gctagtcagg ctagacaaat      660

ggtgcaagcg atgagaacca ttggaactca tcctagctcc agtgctggtc tgaaaaatga      720

tcttcttgaa aatttgcagg cctatcagaa acgaatgggg gtgcagatgc aacggttcaa      780

gtgatcctct cactattgcc gcaaatatca ttgggatctt gcacttgaca ttgtggattc      840

ttgatcgtct ttttttcaaa tgcatttacc gtcgctttaa atacggactg aaaggagggc      900

cttctacgga aggagtgcca aagtctatga gggaagaata tcgaaaggaa cagcagagtg      960

ctgtggatgc tgacgatggt cattttgtca gcatagagct ggagtaaaaa actaccttgt     1020

ttctact                                                               1027


<210>  6
<211>  890
<212>  DNA
<213>  Influenza A virus

<400>  6
agcaaaagca gggtgacaaa aacataatgg atccaaacac tgtgtcaagc tttcaggtag       60

attgctttct ttggcatgtc cgcaaacgag ttgcagacca agaactaggt gatgccccat      120

tccttgatcg gcttcgccga gatcagaaat ccctaagagg aaggggcagt actctcggtc      180

tggacatcaa gacagccaca cgtgctggaa agcagatagt ggagcggatt ctgaaagaag      240

aatccgatga ggcacttaaa atgaccatgg cctctgtacc tgcgtcgcgt tacctaactg      300

acatgactct tgaggaaatg tcaagggact ggtccatgct catacccaag cagaaagtgg      360

caggccctct ttgtatcaga atggaccagg cgatcatgga taagaacatc atactgaaag      420

cgaacttcag tgtgattttt gaccggctgg agactctaat attgctaagg gctttcaccg      480

aagagggagc aattgttggc gaaatttcac cattgccttc tcttccagga catactgctg      540

aggatgtcaa aaatgcagtt ggagtcctca tcggaggact tgaatggaat gataacacag      600

ttcgagtctc tgaaactcta cagagattcg cttggagaag cagtaatgag aatgggagac      660

ctccactcac tccaaaacag aaacgagaaa tggcgggaac aattaggtca gaagtttgaa      720

gaaataagat ggttgattga agaagtgaga cacaaactga agataacaga gaatagtttt      780

gagcaaataa catttatgca agccttacat ctattgcttg aagtggagca agagataaga      840

actttctcgt ttcagcttat ttagtactaa aaaacaccct tgtttctact                 890


<210>  7
<211>  2233
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PR8 segment 3 PTC mutant 1

<400>  7
agcgaaagca ggtactgatc caaaatggaa gattttgtgc gacaatgctt caatccgatg       60

attgtcgagc ttgcggaaaa aacaatgaaa gagtatgggg aggacctgaa aatcgaaaca      120

aacaaatttg cagcaatatg cactcacttg gaagtatgct tcatgtattc agattttcac      180

ttcatcaatg agcaaggcga gtcaataatc gtagaacttg gtgatccaaa tgcacttttg      240

aagcacagat ttgaaataat cgagggaaga gatcgcacaa tggcctggac agtagtaaac      300

agtatttgca acactacagg ggctgagaaa ccaaagtttc taccagattt gtatgattac      360

aaggagaata gattcatcga aattggagta acaaggagag aagttcacat atactatctg      420

gaaaaggcca ataaaattaa atctgagaaa acacacatcc acattttctc gttcactggg      480

gaagaaatgg ccacaaaggc agactacact ctcgatgaag aaagcagggc taggatcaaa      540

accagactat tcaccataag acaagaaatg gccagcagag gcctctggga ttcctttcgt      600

cagtccgaga gaggagaaga gacaattgaa gaaaggtttg aaataacagg aacaatgcgc      660

aagcttgccg accaaagtct cccgccgaac ttctccagcc ttgaaaattt tagagcctat      720

gtggatggat tcgaaccgaa cggctacatt gagggcaagc tgtctcaaat gtccaaagaa      780

gtaaatgcta gaattgaacc ttttttgaaa acaacaccac gaccacttag acttccgaat      840

gggcctccct gttctcagcg gtccaaattc ctactgatgg atgccttaaa attaagcatt      900

gaggacccaa gtcatgaagg agagggaata ccgctatatg atgcaatcaa atgcatgaga      960

acattctttg gatggaagga acccaatgtt gttaaaccac acgaaaaggg aataaatcca     1020

aattatcttc tgtcatggaa gcaagtactg gcagaactgc aggacattga gaatgaggag     1080

aaaattccaa agactaaaaa tatgaagaaa acaagtcagc taaagtgggc acttggtgag     1140

aacatggcac cagaaaaggt agactttgac gactgtaaag atgtaggtga tttgaagcaa     1200

tatgatagtg atgaaccaga attgaggtcg cttgcaagtt ggattcagaa tgagtttaac     1260

aaggcatgcg aactgacaga ttcaagctgg atagagctcg atgagattgg agaagatgtg     1320

gctccaattg aacacattgc aagcatgaga aggaattatt tcacatcaga ggtgtctcac     1380

tgcagagcca cagaatacat aatgaagggg gtgtacatca atactgcctt gcttaatgca     1440

tcttgtgcag caatggatga tttccaatta attccaatga taagcaagtg tagaactaag     1500

gagggaaggc gaaagaccaa cttgtatggt ttcatcataa aaggaagatc ccacttaagg     1560

aatgacaccg acgtggtaaa ctttgtgagc atggagtttt ctctcactga cccaagactt     1620

gaaccacata aatgggagaa gtactgtgtt cttgagatag gagatatgct tataagaagt     1680

gccataggcc aggtttcaag gcccatgttc ttgtatgtga gaacaaatgg aacctcaaaa     1740

attaaaatga aatggggaat ggagatgagg cgttgcctcc tccagtcact tcaacaaatt     1800

gagagtatga ttgaagctga gtcctctgtc aaagagaaag acatgaccaa agagttcttt     1860

gagaacaaat cagaaacatg gcccattgga gagtccccca aaggagtgga ggaaagttcc     1920

attgggaagg tctgcaggac tttattagca aagtcggtat tcaacagctt gtatgcatct     1980

ccacaactag aaggattttc agctgaatca agaaaactgc ttcttatcgt tcaggctctt     2040

agggacaacc ttgaacctgg gacctttgat cttggggggc tatatgaagc aattgaggag     2100

tgcctgatta atgatccctg ggttttgctt aatgcttctt ggttcaactc attccttaca     2160

catgcattga gttagttgtg gcagtgctac tatttgctat ccatactgtc caaaaaagta     2220

ccttgtttct act                                                        2233


<210>  8
<211>  2233
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PR8 segment 3 PTC mutant 2

<400>  8
agcgaaagca ggtactgatc caaaatggaa gattttgtgc gacaatgctt caatccgatg       60

attgtcgagc ttgcggaaaa aacaatgaaa gagtatgggg aggacctgaa aatcgaaaca      120

aacaaatttg cagcaatatg cactcacttg gaagtatgct tcatgtattc agattttcac      180

ttcatcaatg agcaaggcga gtcaataatc gtagaacttg gtgatccaaa tgcacttttg      240

aagcacagat ttgaaataat cgagggaaga gatcgcacaa tggcctggac agtagtaaac      300

agtatttgca acactacagg ggctgagaaa ccaaagtttc taccagattt gtatgattac      360

aaggagaata gattcatcga aattggagta acaaggagag aagttcacat atactatctg      420

gaaaaggcca ataaaattaa atctgagaaa acacacatcc acattttctc gttcactggg      480

gaagaaatgg ccacaaaggc agactacact ctcgatgaag aaagcagggc taggatcaaa      540

accagactat tcaccataag acaagaaatg gccagcagag gcctctggga ttcctttcgt      600

cagtccgaga gaggagaaga gacaattgaa gaaaggtttg aaatcacagg aacaatgagg      660

aagctagccg accaaagtct cccgccgaac ttctccagcc ttgaaaattt tagagcctat      720

gtggatggat tcgaaccgaa cggctacatt gagggcaagc tgtctcaaat gtccaaagaa      780

gtaaatgcta gaattgaacc ttttttgaaa acaacaccac gaccacttag acttccgaat      840

gggcctccct gttctcagcg gtccaaattc ctactgatgg atgccttaaa attaagcatt      900

gaggacccaa gtcatgaagg agagggaata ccgctatatg atgcaatcaa atgcatgaga      960

acattctttg gatggaagga acccaatgtt gttaaaccac acgaaaaggg aataaatcca     1020

aattatcttc tgtcatggaa gcaagtactg gcagaactgc aggacattga gaatgaggag     1080

aaaattccaa agactaaaaa tatgaagaaa acaagtcagc taaagtgggc acttggtgag     1140

aacatggcac cagaaaaggt agactttgac gactgtaaag atgtaggtga tttgaagcaa     1200

tatgatagtg atgaaccaga attgaggtcg cttgcaagtt ggattcagaa tgagtttaac     1260

aaggcatgcg aactgacaga ttcaagctgg atagagctcg atgagattgg agaagatgtg     1320

gctccaattg aacacattgc aagcatgaga aggaattatt tcacatcaga ggtgtctcac     1380

tgcagagcca cagaatacat aatgaagggg gtgtacatca atactgcctt gcttaatgca     1440

tcttgtgcag caatggatga tttccaatta attccaatga taagcaagtg tagaactaag     1500

gagggaaggc gaaagaccaa cttgtatggt ttcatcataa aaggaagatc ccacttaagg     1560

aatgacaccg acgtggtaaa ctttgtgagc atggagtttt ctctcactga cccaagactt     1620

gaaccacata aatgggagaa gtactgtgtt cttgagatag gagatatgct tataagaagt     1680

gccataggcc aggtttcaag gcccatgttc ttgtatgtga gaacaaatgg aacctcaaaa     1740

attaaaatga aatggggaat ggagatgagg cgttgcctcc tccagtcact tcaacaaatt     1800

gagagtatga ttgaagctga gtcctctgtc aaagagaaag acatgaccaa agagttcttt     1860

gagaacaaat cagaaacatg gcccattgga gagtccccca aaggagtgga ggaaagttcc     1920

attgggaagg tctgcaggac tttattagca aagtcggtat tcaacagctt gtatgcatct     1980

ccacaactag aaggattttc agctgaatca agaaaactgc ttcttatcgt tcaggctctt     2040

agggacaacc ttgaacctgg gacctttgat cttggggggc tatatgaagc aattgaggag     2100

tgcctgatta atgatccctg ggttttgctt aatgcttctt ggttcaactc attccttaca     2160

catgcattga gttagttgtg gcagtgctac tatttgctat ccatactgtc caaaaaagta     2220

ccttgtttct act                                                        2233


<210>  9
<211>  2233
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PR8 segment 3 PTC mutant 3

<400>  9
agcgaaagca ggtactgatc caaaatggaa gattttgtgc gacaatgctt caatccgatg       60

attgtcgagc ttgcggaaaa aacaatgaaa gagtatgggg aggacctgaa aatcgaaaca      120

aacaaatttg cagcaatatg cactcacttg gaagtatgct tcatgtattc agattttcac      180

ttcatcaatg agcaaggcga gtcaataatc gtagaacttg gtgatccaaa tgcacttttg      240

aagcacagat ttgaaataat cgagggaaga gatcgcacaa tggcctggac agtagtaaac      300

agtatttgca acactacagg ggctgagaaa ccaaagtttc taccagattt gtatgattac      360

aaggagaata gattcatcga aattggagta acaaggagag aagttcacat atactatctg      420

gaaaaggcca ataaaattaa atctgagaaa acacacatcc acattttctc gttcactggg      480

gaagaaatgg ccacaaaggc agactacact ctcgatgaag aaagcagggc taggatcaaa      540

accagactat tcaccataag acaagaaatg gccagcagag gcctctggga ttcctttcgt      600

cagtccgaga gaggagaaga gacaattgaa gaaaggtttg aaatcacagg aacaatgcgc      660

aagcttgccg accaaagtct cccgccgaac ttctccagcc tagaaaattt tagagcctat      720

gtggatggat tcgaaccgaa cggctacatt gagggcaagc tgtctcaaat gtccaaagaa      780

gtaaatgcta gaattgaacc ttttttgaaa acaacaccac gaccacttag acttccgaat      840

gggcctccct gttctcagcg gtccaaattc ctactgatgg atgccttaaa attaagcatt      900

gaggacccaa gtcatgaagg agagggaata ccgctatatg atgcaatcaa atgcatgaga      960

acattctttg gatggaagga acccaatgtt gttaaaccac acgaaaaggg aataaatcca     1020

aattatcttc tgtcatggaa gcaagtactg gcagaactgc aggacattga gaatgaggag     1080

aaaattccaa agactaaaaa tatgaagaaa acaagtcagc taaagtgggc acttggtgag     1140

aacatggcac cagaaaaggt agactttgac gactgtaaag atgtaggtga tttgaagcaa     1200

tatgatagtg atgaaccaga attgaggtcg cttgcaagtt ggattcagaa tgagtttaac     1260

aaggcatgcg aactgacaga ttcaagctgg atagagctcg atgagattgg agaagatgtg     1320

gctccaattg aacacattgc aagcatgaga aggaattatt tcacatcaga ggtgtctcac     1380

tgcagagcca cagaatacat aatgaagggg gtgtacatca atactgcctt gcttaatgca     1440

tcttgtgcag caatggatga tttccaatta attccaatga taagcaagtg tagaactaag     1500

gagggaaggc gaaagaccaa cttgtatggt ttcatcataa aaggaagatc ccacttaagg     1560

aatgacaccg acgtggtaaa ctttgtgagc atggagtttt ctctcactga cccaagactt     1620

gaaccacata aatgggagaa gtactgtgtt cttgagatag gagatatgct tataagaagt     1680

gccataggcc aggtttcaag gcccatgttc ttgtatgtga gaacaaatgg aacctcaaaa     1740

attaaaatga aatggggaat ggagatgagg cgttgcctcc tccagtcact tcaacaaatt     1800

gagagtatga ttgaagctga gtcctctgtc aaagagaaag acatgaccaa agagttcttt     1860

gagaacaaat cagaaacatg gcccattgga gagtccccca aaggagtgga ggaaagttcc     1920

attgggaagg tctgcaggac tttattagca aagtcggtat tcaacagctt gtatgcatct     1980

ccacaactag aaggattttc agctgaatca agaaaactgc ttcttatcgt tcaggctctt     2040

agggacaacc ttgaacctgg gacctttgat cttggggggc tatatgaagc aattgaggag     2100

tgcctgatta atgatccctg ggttttgctt aatgcttctt ggttcaactc attccttaca     2160

catgcattga gttagttgtg gcagtgctac tatttgctat ccatactgtc caaaaaagta     2220

ccttgtttct act                                                        2233


<210>  10
<211>  2233
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PR8 segment 3 PTC mutant 4

<400>  10
agcgaaagca ggtactgatc caaaatggaa gattttgtgc gacaatgctt caatccgatg       60

attgtcgagc ttgcggaaaa aacaatgaaa gagtatgggg aggacctgaa aatcgaaaca      120

aacaaatttg cagcaatatg cactcacttg gaagtatgct tcatgtattc agattttcac      180

ttcatcaatg agcaaggcga gtcaataatc gtagaacttg gtgatccaaa tgcacttttg      240

aagcacagat ttgaaataat cgagggaaga gatcgcacaa tggcctggac agtagtaaac      300

agtatttgca acactacagg ggctgagaaa ccaaagtttc taccagattt gtatgattac      360

aaggagaata gattcatcga aattggagta acaaggagag aagttcacat atactatctg      420

gaaaaggcca ataaaattaa atctgagaaa acacacatcc acattttctc gttcactggg      480

gaagaaatgg ccacaaaggc agactacact ctcgatgaag aaagcagggc taggatcaaa      540

accagactat tcaccataag acaagaaatg gccagcagag gcctctggga ttcctttcgt      600

cagtccgaga gaggagaaga gacaattgaa gaaaggtttg aaatcacagg aacaatgcgc      660

aagcttgccg accaaagtct cccgccgaac ttctccagcc ttgaaaattt tagagcctat      720

gtagatggat tcgaaccgaa cggctacatt gagggcaagc tgtctcaaat gtccaaagaa      780

gtaaatgcta gaattgaacc ttttttgaaa acaacaccac gaccacttag acttccgaat      840

gggcctccct gttctcagcg gtccaaattc ctactgatgg atgccttaaa attaagcatt      900

gaggacccaa gtcatgaagg agagggaata ccgctatatg atgcaatcaa atgcatgaga      960

acattctttg gatggaagga acccaatgtt gttaaaccac acgaaaaggg aataaatcca     1020

aattatcttc tgtcatggaa gcaagtactg gcagaactgc aggacattga gaatgaggag     1080

aaaattccaa agactaaaaa tatgaagaaa acaagtcagc taaagtgggc acttggtgag     1140

aacatggcac cagaaaaggt agactttgac gactgtaaag atgtaggtga tttgaagcaa     1200

tatgatagtg atgaaccaga attgaggtcg cttgcaagtt ggattcagaa tgagtttaac     1260

aaggcatgcg aactgacaga ttcaagctgg atagagctcg atgagattgg agaagatgtg     1320

gctccaattg aacacattgc aagcatgaga aggaattatt tcacatcaga ggtgtctcac     1380

tgcagagcca cagaatacat aatgaagggg gtgtacatca atactgcctt gcttaatgca     1440

tcttgtgcag caatggatga tttccaatta attccaatga taagcaagtg tagaactaag     1500

gagggaaggc gaaagaccaa cttgtatggt ttcatcataa aaggaagatc ccacttaagg     1560

aatgacaccg acgtggtaaa ctttgtgagc atggagtttt ctctcactga cccaagactt     1620

gaaccacata aatgggagaa gtactgtgtt cttgagatag gagatatgct tataagaagt     1680

gccataggcc aggtttcaag gcccatgttc ttgtatgtga gaacaaatgg aacctcaaaa     1740

attaaaatga aatggggaat ggagatgagg cgttgcctcc tccagtcact tcaacaaatt     1800

gagagtatga ttgaagctga gtcctctgtc aaagagaaag acatgaccaa agagttcttt     1860

gagaacaaat cagaaacatg gcccattgga gagtccccca aaggagtgga ggaaagttcc     1920

attgggaagg tctgcaggac tttattagca aagtcggtat tcaacagctt gtatgcatct     1980

ccacaactag aaggattttc agctgaatca agaaaactgc ttcttatcgt tcaggctctt     2040

agggacaacc ttgaacctgg gacctttgat cttggggggc tatatgaagc aattgaggag     2100

tgcctgatta atgatccctg ggttttgctt aatgcttctt ggttcaactc attccttaca     2160

catgcattga gttagttgtg gcagtgctac tatttgctat ccatactgtc caaaaaagta     2220

ccttgtttct act                                                        2233


<210>  11
<211>  2233
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PR8 segment 3 frameshift mutant

<400>  11
agcgaaagca ggtactgatc caaaatggaa gattttgtgc gacaatgctt caatccgatg       60

attgtcgagc ttgcggaaaa aacaatgaaa gagtatgggg aggacctgaa aatcgaaaca      120

aacaaatttg cagcaatatg cactcacttg gaagtatgct tcatgtattc agattttcac      180

ttcatcaatg agcaaggcga gtcaataatc gtagaacttg gtgatccaaa tgcacttttg      240

aagcacagat ttgaaataat cgagggaaga gatcgcacaa tggcctggac agtagtaaac      300

agtatttgca acactacagg ggctgagaaa ccaaagtttc taccagattt gtatgattac      360

aaggagaata gattcatcga aattggagta acaaggagag aagttcacat atactatctg      420

gaaaaggcca ataaaattaa atctgagaaa acacacatcc acattttctc gttcactggg      480

gaagaaatgg ccacaaaggc agactacact ctcgatgaag aaagcagggc taggatcaaa      540

accagactat tcaccataag acaagaaatg gccagcagag gcctctggga tagcttcaga      600

cagtccgaga gaggagaaga gacaattgaa gaaaggtttg aaatcacagg aacaatgcgc      660

aagcttgccg accaaagtct cccgccgaac ttctccagcc ttgaaaattt tagagcctat      720

gtggatggat tcgaaccgaa cggctacatt gagggcaagc tgtctcaaat gtccaaagaa      780

gtaaatgcta gaattgaacc ttttttgaaa acaacaccac gaccacttag acttccgaat      840

gggcctccct gttctcagcg gtccaaattc ctactgatgg atgccttaaa attaagcatt      900

gaggacccaa gtcatgaagg agagggaata ccgctatatg atgcaatcaa atgcatgaga      960

acattctttg gatggaagga acccaatgtt gttaaaccac acgaaaaggg aataaatcca     1020

aattatcttc tgtcatggaa gcaagtactg gcagaactgc aggacattga gaatgaggag     1080

aaaattccaa agactaaaaa tatgaagaaa acaagtcagc taaagtgggc acttggtgag     1140

aacatggcac cagaaaaggt agactttgac gactgtaaag atgtaggtga tttgaagcaa     1200

tatgatagtg atgaaccaga attgaggtcg cttgcaagtt ggattcagaa tgagtttaac     1260

aaggcatgcg aactgacaga ttcaagctgg atagagctcg atgagattgg agaagatgtg     1320

gctccaattg aacacattgc aagcatgaga aggaattatt tcacatcaga ggtgtctcac     1380

tgcagagcca cagaatacat aatgaagggg gtgtacatca atactgcctt gcttaatgca     1440

tcttgtgcag caatggatga tttccaatta attccaatga taagcaagtg tagaactaag     1500

gagggaaggc gaaagaccaa cttgtatggt ttcatcataa aaggaagatc ccacttaagg     1560

aatgacaccg acgtggtaaa ctttgtgagc atggagtttt ctctcactga cccaagactt     1620

gaaccacata aatgggagaa gtactgtgtt cttgagatag gagatatgct tataagaagt     1680

gccataggcc aggtttcaag gcccatgttc ttgtatgtga gaacaaatgg aacctcaaaa     1740

attaaaatga aatggggaat ggagatgagg cgttgcctcc tccagtcact tcaacaaatt     1800

gagagtatga ttgaagctga gtcctctgtc aaagagaaag acatgaccaa agagttcttt     1860

gagaacaaat cagaaacatg gcccattgga gagtccccca aaggagtgga ggaaagttcc     1920

attgggaagg tctgcaggac tttattagca aagtcggtat tcaacagctt gtatgcatct     1980

ccacaactag aaggattttc agctgaatca agaaaactgc ttcttatcgt tcaggctctt     2040

agggacaacc ttgaacctgg gacctttgat cttggggggc tatatgaagc aattgaggag     2100

tgcctgatta atgatccctg ggttttgctt aatgcttctt ggttcaactc attccttaca     2160

catgcattga gttagttgtg gcagtgctac tatttgctat ccatactgtc caaaaaagta     2220

ccttgtttct act                                                        2233


<210>  12
<211>  5212
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector plasmid pHW2000_RF483

<400>  12
atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt       60

ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat      120

taatacgact cactataggg agacccaagc tgttaacgct agcagttaac cggagtactg      180

gtcgacctcc gaagttgggg gggagagacg gtaccgtctc cagcgaaagc aggtactgat      240

ccaaaatgga agattttgtg cgacaatgct tcaatccgat gattgtcgag cttgcggaaa      300

aaacaatgaa agagtatggg gaggacctga aaatcgaaac aaacaaattt gcagcaatat      360

gcactcactt ggaagtatgc ttcatgtatt cagattttca cttcatcaat gagcaaggcg      420

agtcaataat cgtagaactt ggtgatccaa atgcactttt gaagcacaga tttgaaataa      480

tcgagggaag agatcgcaca atggcctgga cagtagtaaa cagtatttgc aacactacag      540

gggctgagaa accaaagttt ctaccagatt tgtatgatta caaggagaat agattcatcg      600

aaattggagt aacaaggaga gaagttcaca tatactatct ggaaaaggcc aataaaatta      660

aatctgagaa aacacacatc cacattttct cgttcactgg ggaagaaatg gccacaaagg      720

cagactacac tctcgatgaa gaaagcaggg ctaggatcaa aaccagacta ttcaccataa      780

gacaagaaat ggccagcaga ggcctctggg atagcttcag acagtccgag agaggagaag      840

agacaattga agaaaggttt gaaatcacag gaacaatgcg caagcttgcc gaccaaagtc      900

tcccgccgaa cttctccagc cttgaaaatt ttagagccta tgtggatgga ttcgaaccga      960

acggctacat tgagggcaag ctgtctcaaa tgtccaaaga agtaaatgct agaattgaac     1020

cttttttgaa aacaacacca cgaccactta gacttccgaa tgggcctccc tgttctcagc     1080

ggtccaaatt cctactgatg gatgccttaa aattaagcat tgaggaccca agtcatgaag     1140

gagagggaat accgctatat gatgcaatca aatgcatgag aacattcttt ggatggaagg     1200

aacccaatgt tgttaaacca cacgaaaagg gaataaatcc aaattatctt ctgtcatgga     1260

agcaagtact ggcagaactg caggacattg agaatgagga gaaaattcca aagactaaaa     1320

atatgaagaa aacaagtcag ctaaagtggg cacttggtga gaacatggca ccagaaaagg     1380

tagactttga cgactgtaaa gatgtaggtg atttgaagca atatgatagt gatgaaccag     1440

aattgaggtc gcttgcaagt tggattcaga atgagtttaa caaggcatgc gaactgacag     1500

attcaagctg gatagagctc gatgagattg gagaagatgt ggctccaatt gaacacattg     1560

caagcatgag aaggaattat ttcacatcag aggtgtctca ctgcagagcc acagaataca     1620

taatgaaggg ggtgtacatc aatactgcct tgcttaatgc atcttgtgca gcaatggatg     1680

atttccaatt aattccaatg ataagcaagt gtagaactaa ggagggaagg cgaaagacca     1740

acttgtatgg tttcatcata aaaggaagat cccacttaag gaatgacacc gacgtggtaa     1800

actttgtgag catggagttt tctctcactg acccaagact tgaaccacat aaatgggaga     1860

agtactgtgt tcttgagata ggagatatgc ttataagaag tgccataggc caggtttcaa     1920

ggcccatgtt cttgtatgtg agaacaaatg gaacctcaaa aattaaaatg aaatggggaa     1980

tggagatgag gcgttgcctc ctccagtcac ttcaacaaat tgagagtatg attgaagctg     2040

agtcctctgt caaagagaaa gacatgacca aagagttctt tgagaacaaa tcagaaacat     2100

ggcccattgg agagtccccc aaaggagtgg aggaaagttc cattgggaag gtctgcagga     2160

ctttattagc aaagtcggta ttcaacagct tgtatgcatc tccacaacta gaaggatttt     2220

cagctgaatc aagaaaactg cttcttatcg ttcaggctct tagggacaac cttgaacctg     2280

ggacctttga tcttgggggg ctatatgaag caattgagga gtgcctgatt aatgatccct     2340

gggttttgct taatgcttct tggttcaact cattccttac acatgcattg agttagttgt     2400

ggcagtgcta ctatttgcta tccatactgt ccaaaaaagt accttgtttc tactaataac     2460

ccggcggccc aaaatgccga ctcggagcga aagatatacc tcccccgggg ccgggaggtc     2520

gcgtcaccga ccacgccgcc ggcccaggcg acgcgcgaca cggacacctg tccccaaaaa     2580

cgccaccatc gcagccacac acggagcgcc cggggccctc tggtcaaccc caggacacac     2640

gcgggagcag cgccgggccg gggacgccct cccggcggtc acctaaatgc tagagctcgc     2700

tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg     2760

ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt     2820

gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc     2880

aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct     2940

tctgaggcgg aaagaaccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt     3000

tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc     3060

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg     3120

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg     3180

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac     3240

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg     3300

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct     3360

ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg     3420

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct     3480

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac     3540

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt     3600

tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc     3660

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca     3720

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat     3780

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac     3840

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt     3900

aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc     3960

aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg     4020

cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg     4080

ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc     4140

cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta     4200

ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg     4260

ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct     4320

ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta     4380

gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg     4440

ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga     4500

ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt     4560

gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca     4620

ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt     4680

cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt     4740

ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga     4800

aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt     4860

gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc     4920

gcacatttcc ccgaaaagtg ccacctgacg tcgatatgcc aagtacgccc cctattgacg     4980

tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc     5040

ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc     5100

agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca     5160

ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aa             5212


<210>  13
<211>  252
<212>  PRT
<213>  Influenza A virus

<400>  13

Met Glu Asp Phe Val Arg Gln Cys Phe Asn Pro Met Ile Val Glu Leu 
1               5                   10                  15      


Ala Glu Lys Thr Met Lys Glu Tyr Gly Glu Asp Leu Lys Ile Glu Thr 
            20                  25                  30          


Asn Lys Phe Ala Ala Ile Cys Thr His Leu Glu Val Cys Phe Met Tyr 
        35                  40                  45              


Ser Asp Phe His Phe Ile Asn Glu Gln Gly Glu Ser Ile Ile Val Glu 
    50                  55                  60                  


Leu Gly Asp Pro Asn Ala Leu Leu Lys His Arg Phe Glu Ile Ile Glu 
65                  70                  75                  80  


Gly Arg Asp Arg Thr Met Ala Trp Thr Val Val Asn Ser Ile Cys Asn 
                85                  90                  95      


Thr Thr Gly Ala Glu Lys Pro Lys Phe Leu Pro Asp Leu Tyr Asp Tyr 
            100                 105                 110         


Lys Glu Asn Arg Phe Ile Glu Ile Gly Val Thr Arg Arg Glu Val His 
        115                 120                 125             


Ile Tyr Tyr Leu Glu Lys Ala Asn Lys Ile Lys Ser Glu Lys Thr His 
    130                 135                 140                 


Ile His Ile Phe Ser Phe Thr Gly Glu Glu Met Ala Thr Lys Ala Asp 
145                 150                 155                 160 


Tyr Thr Leu Asp Glu Glu Ser Arg Ala Arg Ile Lys Thr Arg Leu Phe 
                165                 170                 175     


Thr Ile Arg Gln Glu Met Ala Ser Arg Gly Leu Trp Asp Ser Phe Val 
            180                 185                 190         


Ser Pro Arg Glu Glu Lys Arg Gln Leu Lys Lys Gly Leu Lys Ser Gln 
        195                 200                 205             


Glu Gln Cys Ala Ser Leu Pro Thr Lys Val Ser Arg Arg Thr Ser Pro 
    210                 215                 220                 


Ala Leu Lys Ile Leu Glu Pro Met Trp Met Asp Ser Asn Arg Thr Ala 
225                 230                 235                 240 


Thr Leu Arg Ala Ser Cys Leu Lys Cys Pro Lys Lys 
                245                 250         


<210>  14
<211>  10
<212>  DNA
<213>  Influenza A virus

<400>  14
UCC UUU CGU C                                                           10


<210>  15
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated frameshift region of PR8 segment 3

<400>  15
AGC UUC AGA 								9


<210>  16
<211>  759
<212>  PRT
<213>  Influenza A virus

<400>  16

Met Glu Arg Ile Lys Glu Leu Arg Asn Leu Met Ser Gln Ser Arg Thr 
1               5                   10                  15      


Arg Glu Ile Leu Thr Lys Thr Thr Val Asp His Met Ala Ile Ile Lys 
            20                  25                  30          


Lys Tyr Thr Ser Gly Arg Gln Glu Lys Asn Pro Ala Leu Arg Met Lys 
        35                  40                  45              


Trp Met Met Ala Met Lys Tyr Pro Ile Thr Ala Asp Lys Arg Ile Thr 
    50                  55                  60                  


Glu Met Ile Pro Glu Arg Asn Glu Gln Gly Gln Thr Leu Trp Ser Lys 
65                  70                  75                  80  


Met Asn Asp Ala Gly Ser Asp Arg Val Met Val Ser Pro Leu Ala Val 
                85                  90                  95      


Thr Trp Trp Asn Arg Asn Gly Pro Ile Thr Asn Thr Val His Tyr Pro 
            100                 105                 110         


Lys Ile Tyr Lys Thr Tyr Phe Glu Arg Val Glu Arg Leu Lys His Gly 
        115                 120                 125             


Thr Phe Gly Pro Val His Phe Arg Asn Gln Val Lys Ile Arg Arg Arg 
    130                 135                 140                 


Val Asp Ile Asn Pro Gly His Ala Asp Leu Ser Ala Lys Glu Ala Gln 
145                 150                 155                 160 


Asp Val Ile Met Glu Val Val Phe Pro Asn Glu Val Gly Ala Arg Ile 
                165                 170                 175     


Leu Thr Ser Glu Ser Gln Leu Thr Ile Thr Lys Glu Lys Lys Glu Glu 
            180                 185                 190         


Leu Gln Asp Cys Lys Ile Ser Pro Leu Met Val Ala Tyr Met Leu Glu 
        195                 200                 205             


Arg Glu Leu Val Arg Lys Thr Arg Phe Leu Pro Val Ala Gly Gly Thr 
    210                 215                 220                 


Ser Ser Val Tyr Ile Glu Val Leu His Leu Thr Gln Gly Thr Cys Trp 
225                 230                 235                 240 


Glu Gln Met Tyr Thr Pro Gly Gly Glu Val Arg Asn Asp Asp Val Asp 
                245                 250                 255     


Gln Ser Leu Ile Ile Ala Ala Arg Asn Ile Val Arg Arg Ala Ala Val 
            260                 265                 270         


Ser Ala Asp Pro Leu Ala Ser Leu Leu Glu Met Cys His Ser Thr Gln 
        275                 280                 285             


Ile Gly Gly Ile Arg Met Val Asp Ile Leu Arg Gln Asn Pro Thr Glu 
    290                 295                 300                 


Glu Gln Ala Val Asp Ile Cys Lys Ala Ala Met Gly Leu Arg Ile Ser 
305                 310                 315                 320 


Ser Ser Phe Ser Phe Gly Gly Phe Thr Phe Lys Arg Thr Ser Gly Ser 
                325                 330                 335     


Ser Val Lys Arg Glu Glu Glu Val Leu Thr Gly Asn Leu Gln Thr Leu 
            340                 345                 350         


Lys Ile Arg Val His Glu Gly Tyr Glu Glu Phe Thr Met Val Gly Arg 
        355                 360                 365             


Arg Ala Thr Ala Ile Leu Arg Lys Ala Thr Arg Arg Leu Ile Gln Leu 
    370                 375                 380                 


Ile Val Ser Gly Arg Asp Glu Gln Ser Ile Ala Glu Ala Ile Ile Val 
385                 390                 395                 400 


Ala Met Val Phe Ser Gln Glu Asp Cys Met Ile Lys Ala Val Arg Gly 
                405                 410                 415     


Asp Leu Asn Phe Val Asn Arg Ala Asn Gln Arg Leu Asn Pro Met His 
            420                 425                 430         


Gln Leu Leu Arg His Phe Gln Lys Asp Ala Lys Val Leu Phe Gln Asn 
        435                 440                 445             


Trp Gly Val Glu Pro Ile Asp Asn Val Met Gly Met Ile Gly Ile Leu 
    450                 455                 460                 


Pro Asp Met Thr Pro Ser Ile Glu Met Ser Met Arg Gly Val Arg Ile 
465                 470                 475                 480 


Ser Lys Met Gly Val Asp Glu Tyr Ser Ser Thr Glu Arg Val Val Val 
                485                 490                 495     


Ser Ile Asp Arg Phe Leu Arg Ile Arg Asp Gln Arg Gly Asn Val Leu 
            500                 505                 510         


Leu Ser Pro Glu Glu Val Ser Glu Thr Gln Gly Thr Glu Lys Leu Thr 
        515                 520                 525             


Ile Thr Tyr Ser Ser Ser Met Met Trp Glu Ile Asn Gly Pro Glu Ser 
    530                 535                 540                 


Val Leu Val Asn Thr Tyr Gln Trp Ile Ile Arg Asn Trp Glu Thr Val 
545                 550                 555                 560 


Lys Ile Gln Trp Ser Gln Asn Pro Thr Met Leu Tyr Asn Lys Met Glu 
                565                 570                 575     


Phe Glu Pro Phe Gln Ser Leu Val Pro Lys Ala Ile Arg Gly Gln Tyr 
            580                 585                 590         


Ser Gly Phe Val Arg Thr Leu Phe Gln Gln Met Arg Asp Val Leu Gly 
        595                 600                 605             


Thr Phe Asp Thr Ala Gln Ile Ile Lys Leu Leu Pro Phe Ala Ala Ala 
    610                 615                 620                 


Pro Pro Lys Gln Ser Arg Met Gln Phe Ser Ser Phe Thr Val Asn Val 
625                 630                 635                 640 


Arg Gly Ser Gly Met Arg Ile Leu Val Arg Gly Asn Ser Pro Val Phe 
                645                 650                 655     


Asn Tyr Asn Lys Ala Thr Lys Arg Leu Thr Val Leu Gly Lys Asp Ala 
            660                 665                 670         


Gly Thr Leu Thr Glu Asp Pro Asp Glu Gly Thr Ala Gly Val Glu Ser 
        675                 680                 685             


Ala Val Leu Arg Gly Phe Leu Ile Leu Gly Lys Glu Asn Lys Arg Tyr 
    690                 695                 700                 


Gly Pro Ala Leu Ser Ile Asn Glu Leu Ser Asn Leu Ala Lys Gly Glu 
705                 710                 715                 720 


Lys Ala Asn Val Leu Ile Gly Gln Gly Asp Val Val Leu Val Met Lys 
                725                 730                 735     


Arg Lys Arg Asp Ser Ser Ile Leu Thr Asp Ser Gln Thr Ala Thr Lys 
            740                 745                 750         


Arg Ile Arg Met Ala Ile Asn 
        755                 


<210>  17
<211>  757
<212>  PRT
<213>  Influenza A virus

<400>  17

Met Asp Val Asn Pro Thr Leu Leu Phe Leu Lys Val Pro Ala Gln Asn 
1               5                   10                  15      


Ala Ile Ser Thr Thr Phe Pro Tyr Thr Gly Asp Pro Pro Tyr Ser His 
            20                  25                  30          


Gly Thr Gly Thr Gly Tyr Thr Met Asp Thr Val Asn Arg Thr His Gln 
        35                  40                  45              


Tyr Ser Glu Lys Gly Arg Trp Thr Thr Asn Thr Glu Thr Gly Ala Pro 
    50                  55                  60                  


Gln Leu Asn Pro Ile Asp Gly Pro Leu Pro Glu Asp Asn Glu Pro Ser 
65                  70                  75                  80  


Gly Tyr Ala Gln Thr Asp Cys Val Leu Glu Ala Met Ala Phe Leu Glu 
                85                  90                  95      


Glu Ser His Pro Gly Ile Phe Glu Asn Ser Cys Ile Glu Thr Met Glu 
            100                 105                 110         


Val Val Gln Gln Thr Arg Val Asp Lys Leu Thr Gln Gly Arg Gln Thr 
        115                 120                 125             


Tyr Asp Trp Thr Leu Asn Arg Asn Gln Pro Ala Ala Thr Ala Leu Ala 
    130                 135                 140                 


Asn Thr Ile Glu Val Phe Arg Ser Asn Gly Leu Thr Ala Asn Glu Ser 
145                 150                 155                 160 


Gly Arg Leu Ile Asp Phe Leu Lys Asp Val Met Glu Ser Met Asn Lys 
                165                 170                 175     


Glu Glu Met Gly Ile Thr Thr His Phe Gln Arg Lys Arg Arg Val Arg 
            180                 185                 190         


Asp Asn Met Thr Lys Lys Met Ile Thr Gln Arg Thr Met Gly Lys Lys 
        195                 200                 205             


Lys Gln Arg Leu Asn Lys Arg Ser Tyr Leu Ile Arg Ala Leu Thr Leu 
    210                 215                 220                 


Asn Thr Met Thr Lys Asp Ala Glu Arg Gly Lys Leu Lys Arg Arg Ala 
225                 230                 235                 240 


Ile Ala Thr Pro Gly Met Gln Ile Arg Gly Phe Val Tyr Phe Val Glu 
                245                 250                 255     


Thr Leu Ala Arg Ser Ile Cys Glu Lys Leu Glu Gln Ser Gly Leu Pro 
            260                 265                 270         


Val Gly Gly Asn Glu Lys Lys Ala Lys Leu Ala Asn Val Val Arg Lys 
        275                 280                 285             


Met Met Thr Asn Ser Gln Asp Thr Glu Leu Ser Phe Thr Ile Thr Gly 
    290                 295                 300                 


Asp Asn Thr Lys Trp Asn Glu Asn Gln Asn Pro Arg Met Phe Leu Ala 
305                 310                 315                 320 


Met Ile Thr Tyr Met Thr Arg Asn Gln Pro Glu Trp Phe Arg Asn Val 
                325                 330                 335     


Leu Ser Ile Ala Pro Ile Met Phe Ser Asn Lys Met Ala Arg Leu Gly 
            340                 345                 350         


Lys Gly Tyr Met Phe Glu Ser Lys Ser Met Lys Leu Arg Thr Gln Ile 
        355                 360                 365             


Pro Ala Glu Met Leu Ala Ser Ile Asp Leu Lys Tyr Phe Asn Asp Ser 
    370                 375                 380                 


Thr Arg Lys Lys Ile Glu Lys Ile Arg Ser Leu Leu Ile Glu Gly Thr 
385                 390                 395                 400 


Ala Ser Leu Ser Pro Gly Met Met Met Gly Met Phe Asn Met Leu Ser 
                405                 410                 415     


Thr Val Leu Gly Val Ser Ile Leu Asn Leu Gly Gln Lys Arg Tyr Thr 
            420                 425                 430         


Lys Thr Thr Tyr Trp Trp Asp Gly Leu Gln Ser Ser Asp Asp Phe Ala 
        435                 440                 445             


Leu Ile Val Asn Ala Pro Asn His Glu Gly Ile Gln Ala Gly Val Asp 
    450                 455                 460                 


Arg Phe Tyr Arg Thr Cys Lys Leu Leu Gly Ile Asn Met Ser Lys Lys 
465                 470                 475                 480 


Lys Ser Tyr Ile Asn Arg Thr Gly Thr Phe Glu Phe Thr Ser Phe Phe 
                485                 490                 495     


Tyr Arg Tyr Gly Phe Val Ala Asn Phe Ser Met Glu Leu Pro Ser Phe 
            500                 505                 510         


Gly Val Ser Gly Ile Asn Glu Ser Ala Asp Met Ser Ile Gly Val Thr 
        515                 520                 525             


Val Ile Lys Asn Asn Met Ile Asn Asn Asp Leu Gly Pro Ala Thr Ala 
    530                 535                 540                 


Gln Met Ala Leu Gln Leu Phe Ile Lys Asp Tyr Arg Tyr Thr Tyr Arg 
545                 550                 555                 560 


Cys His Arg Gly Asp Thr Gln Ile Gln Thr Arg Arg Ser Phe Glu Ile 
                565                 570                 575     


Lys Lys Leu Trp Glu Gln Thr Arg Ser Lys Ala Gly Leu Leu Val Ser 
            580                 585                 590         


Asp Gly Gly Pro Asn Leu Tyr Asn Ile Arg Asn Leu His Ile Pro Glu 
        595                 600                 605             


Val Cys Leu Lys Trp Glu Leu Met Asp Glu Asp Tyr Gln Gly Arg Leu 
    610                 615                 620                 


Cys Asn Pro Leu Asn Pro Phe Val Ser His Lys Glu Ile Glu Ser Met 
625                 630                 635                 640 


Asn Asn Ala Val Met Met Pro Ala His Gly Pro Ala Lys Asn Met Glu 
                645                 650                 655     


Tyr Asp Ala Val Ala Thr Thr His Ser Trp Ile Pro Lys Arg Asn Arg 
            660                 665                 670         


Ser Ile Leu Asn Thr Ser Gln Arg Gly Val Leu Glu Asp Glu Gln Met 
        675                 680                 685             


Tyr Gln Arg Cys Cys Asn Leu Phe Glu Lys Phe Phe Pro Ser Ser Ser 
    690                 695                 700                 


Tyr Arg Arg Pro Val Gly Ile Ser Ser Met Val Glu Ala Met Val Ser 
705                 710                 715                 720 


Arg Ala Arg Ile Asp Ala Arg Ile Asp Phe Glu Ser Gly Arg Ile Lys 
                725                 730                 735     


Lys Glu Glu Phe Thr Glu Ile Met Lys Ile Cys Ser Thr Ile Glu Glu 
            740                 745                 750         


Leu Arg Arg Gln Lys 
        755         


<210>  18
<211>  498
<212>  PRT
<213>  Influenza A virus

<400>  18

Met Ala Ser Gln Gly Thr Lys Arg Ser Tyr Glu Gln Met Glu Thr Asp 
1               5                   10                  15      


Gly Glu Arg Gln Asn Ala Thr Glu Ile Arg Ala Ser Val Gly Lys Met 
            20                  25                  30          


Ile Gly Gly Ile Gly Arg Phe Tyr Ile Gln Met Cys Thr Glu Leu Lys 
        35                  40                  45              


Leu Ser Asp Tyr Glu Gly Arg Leu Ile Gln Asn Ser Leu Thr Ile Glu 
    50                  55                  60                  


Arg Met Val Leu Ser Ala Phe Asp Glu Arg Arg Asn Lys Tyr Leu Glu 
65                  70                  75                  80  


Glu His Pro Ser Ala Gly Lys Asp Pro Lys Lys Thr Gly Gly Pro Ile 
                85                  90                  95      


Tyr Arg Arg Val Asn Gly Lys Trp Met Arg Glu Leu Ile Leu Tyr Asp 
            100                 105                 110         


Lys Glu Glu Ile Arg Arg Ile Trp Arg Gln Ala Asn Asn Gly Asp Asp 
        115                 120                 125             


Ala Thr Ala Gly Leu Thr His Met Met Ile Trp His Ser Asn Leu Asn 
    130                 135                 140                 


Asp Ala Thr Tyr Gln Arg Thr Arg Ala Leu Val Arg Thr Gly Met Asp 
145                 150                 155                 160 


Pro Arg Met Cys Ser Leu Met Gln Gly Ser Thr Leu Pro Arg Arg Ser 
                165                 170                 175     


Gly Ala Ala Gly Ala Ala Val Lys Gly Val Gly Thr Met Val Met Glu 
            180                 185                 190         


Leu Val Arg Met Ile Lys Arg Gly Ile Asn Asp Arg Asn Phe Trp Arg 
        195                 200                 205             


Gly Glu Asn Gly Arg Lys Thr Arg Ile Ala Tyr Glu Arg Met Cys Asn 
    210                 215                 220                 


Ile Leu Lys Gly Lys Phe Gln Thr Ala Ala Gln Lys Ala Met Met Asp 
225                 230                 235                 240 


Gln Val Arg Glu Ser Arg Asn Pro Gly Asn Ala Glu Phe Glu Asp Leu 
                245                 250                 255     


Thr Phe Leu Ala Arg Ser Ala Leu Ile Leu Arg Gly Ser Val Ala His 
            260                 265                 270         


Lys Ser Cys Leu Pro Ala Cys Val Tyr Gly Pro Ala Val Ala Ser Gly 
        275                 280                 285             


Tyr Asp Phe Glu Arg Glu Gly Tyr Ser Leu Val Gly Ile Asp Pro Phe 
    290                 295                 300                 


Arg Leu Leu Gln Asn Ser Gln Val Tyr Ser Leu Ile Arg Pro Asn Glu 
305                 310                 315                 320 


Asn Pro Ala His Lys Ser Gln Leu Val Trp Met Ala Cys His Ser Ala 
                325                 330                 335     


Ala Phe Glu Asp Leu Arg Val Leu Ser Phe Ile Lys Gly Thr Lys Val 
            340                 345                 350         


Leu Pro Arg Gly Lys Leu Ser Thr Arg Gly Val Gln Ile Ala Ser Asn 
        355                 360                 365             


Glu Asn Met Glu Thr Met Glu Ser Ser Thr Leu Glu Leu Arg Ser Arg 
    370                 375                 380                 


Tyr Trp Ala Ile Arg Thr Arg Ser Gly Gly Asn Thr Asn Gln Gln Arg 
385                 390                 395                 400 


Ala Ser Ala Gly Gln Ile Ser Ile Gln Pro Thr Phe Ser Val Gln Arg 
                405                 410                 415     


Asn Leu Pro Phe Asp Arg Thr Thr Ile Met Ala Ala Phe Asn Gly Asn 
            420                 425                 430         


Thr Glu Gly Arg Thr Ser Asp Met Arg Thr Glu Ile Ile Arg Met Met 
        435                 440                 445             


Glu Ser Ala Arg Pro Glu Asp Val Ser Phe Gln Gly Arg Gly Val Phe 
    450                 455                 460                 


Glu Leu Ser Asp Glu Lys Ala Ala Ser Pro Ile Val Pro Ser Phe Asp 
465                 470                 475                 480 


Met Ser Asn Glu Gly Ser Tyr Phe Phe Gly Asp Asn Ala Glu Glu Tyr 
                485                 490                 495     


Asp Asn 
        


<210>  19
<211>  252
<212>  PRT
<213>  Influenza A virus

<400>  19

Met Ser Leu Leu Thr Glu Val Glu Thr Tyr Val Leu Ser Ile Ile Pro 
1               5                   10                  15      


Ser Gly Pro Leu Lys Ala Glu Ile Ala Gln Arg Leu Glu Asp Val Phe 
            20                  25                  30          


Ala Gly Lys Asn Thr Asp Leu Glu Val Leu Met Glu Trp Leu Lys Thr 
        35                  40                  45              


Arg Pro Ile Leu Ser Pro Leu Thr Lys Gly Ile Leu Gly Phe Val Phe 
    50                  55                  60                  


Thr Leu Thr Val Pro Ser Glu Arg Gly Leu Gln Arg Arg Arg Phe Val 
65                  70                  75                  80  


Gln Asn Ala Leu Asn Gly Asn Gly Asp Pro Asn Asn Met Asp Lys Ala 
                85                  90                  95      


Val Lys Leu Tyr Arg Lys Leu Lys Arg Glu Ile Thr Phe His Gly Ala 
            100                 105                 110         


Lys Glu Ile Ser Leu Ser Tyr Ser Ala Gly Ala Leu Ala Ser Cys Met 
        115                 120                 125             


Gly Leu Ile Tyr Asn Arg Met Gly Ala Val Thr Thr Glu Val Ala Phe 
    130                 135                 140                 


Gly Leu Val Cys Ala Thr Cys Glu Gln Ile Ala Asp Ser Gln His Arg 
145                 150                 155                 160 


Ser His Arg Gln Met Val Thr Thr Thr Asn Pro Leu Ile Arg His Glu 
                165                 170                 175     


Asn Arg Met Val Leu Ala Ser Thr Thr Ala Lys Ala Met Glu Gln Met 
            180                 185                 190         


Ala Gly Ser Ser Glu Gln Ala Ala Glu Ala Met Glu Val Ala Ser Gln 
        195                 200                 205             


Ala Arg Gln Met Val Gln Ala Met Arg Thr Ile Gly Thr His Pro Ser 
    210                 215                 220                 


Ser Ser Ala Gly Leu Lys Asn Asp Leu Leu Glu Asn Leu Gln Ala Tyr 
225                 230                 235                 240 


Gln Lys Arg Met Gly Val Gln Met Gln Arg Phe Lys 
                245                 250         


<210>  20
<211>  97
<212>  PRT
<213>  Influenza A virus

<400>  20

Met Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly 
1               5                   10                  15      


Cys Arg Cys Asn Gly Ser Ser Asp Pro Leu Thr Ile Ala Ala Asn Ile 
            20                  25                  30          


Ile Gly Ile Leu His Leu Thr Leu Trp Ile Leu Asp Arg Leu Phe Phe 
        35                  40                  45              


Lys Cys Ile Tyr Arg Arg Phe Lys Tyr Gly Leu Lys Gly Gly Pro Ser 
    50                  55                  60                  


Thr Glu Gly Val Pro Lys Ser Met Arg Glu Glu Tyr Arg Lys Glu Gln 
65                  70                  75                  80  


Gln Ser Ala Val Asp Ala Asp Asp Gly His Phe Val Ser Ile Glu Leu 
                85                  90                  95      


Glu 
    


<210>  21
<211>  230
<212>  PRT
<213>  Influenza A virus

<400>  21

Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 
1               5                   10                  15      


His Val Arg Lys Arg Val Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 
            20                  25                  30          


Leu Asp Arg Leu Arg Arg Asp Gln Lys Ser Leu Arg Gly Arg Gly Ser 
        35                  40                  45              


Thr Leu Gly Leu Asp Ile Lys Thr Ala Thr Arg Ala Gly Lys Gln Ile 
    50                  55                  60                  


Val Glu Arg Ile Leu Lys Glu Glu Ser Asp Glu Ala Leu Lys Met Thr 
65                  70                  75                  80  


Met Ala Ser Val Pro Ala Ser Arg Tyr Leu Thr Asp Met Thr Leu Glu 
                85                  90                  95      


Glu Met Ser Arg Asp Trp Ser Met Leu Ile Pro Lys Gln Lys Val Ala 
            100                 105                 110         


Gly Pro Leu Cys Ile Arg Met Asp Gln Ala Ile Met Asp Lys Asn Ile 
        115                 120                 125             


Ile Leu Lys Ala Asn Phe Ser Val Ile Phe Asp Arg Leu Glu Thr Leu 
    130                 135                 140                 


Ile Leu Leu Arg Ala Phe Thr Glu Glu Gly Ala Ile Val Gly Glu Ile 
145                 150                 155                 160 


Ser Pro Leu Pro Ser Leu Pro Gly His Thr Ala Glu Asp Val Lys Asn 
                165                 170                 175     


Ala Val Gly Val Leu Ile Gly Gly Leu Glu Trp Asn Asp Asn Thr Val 
            180                 185                 190         


Arg Val Ser Glu Thr Leu Gln Arg Phe Ala Trp Arg Ser Ser Asn Glu 
        195                 200                 205             


Asn Gly Arg Pro Pro Leu Thr Pro Lys Gln Lys Arg Glu Met Ala Gly 
    210                 215                 220                 


Thr Ile Arg Ser Glu Val 
225                 230 


<210>  22
<211>  121
<212>  PRT
<213>  Influenza A virus

<400>  22

Met Asp Pro Asn Thr Val Ser Ser Phe Gln Asp Ile Leu Leu Arg Met 
1               5                   10                  15      


Ser Lys Met Gln Leu Glu Ser Ser Ser Glu Asp Leu Asn Gly Met Ile 
            20                  25                  30          


Thr Gln Phe Glu Ser Leu Lys Leu Tyr Arg Asp Ser Leu Gly Glu Ala 
        35                  40                  45              


Val Met Arg Met Gly Asp Leu His Ser Leu Gln Asn Arg Asn Glu Lys 
    50                  55                  60                  


Trp Arg Glu Gln Leu Gly Gln Lys Phe Glu Glu Ile Arg Trp Leu Ile 
65                  70                  75                  80  


Glu Glu Val Arg His Lys Leu Lys Ile Thr Glu Asn Ser Phe Glu Gln 
                85                  90                  95      


Ile Thr Phe Met Gln Ala Leu His Leu Leu Leu Glu Val Glu Gln Glu 
            100                 105                 110         


Ile Arg Thr Phe Ser Phe Gln Leu Ile 
        115                 120     


<210>  23
<211>  716
<212>  PRT
<213>  Influenza A virus

<400>  23

Met Glu Asp Phe Val Arg Gln Cys Phe Asn Pro Met Ile Val Glu Leu 
1               5                   10                  15      


Ala Glu Lys Thr Met Lys Glu Tyr Gly Glu Asp Leu Lys Ile Glu Thr 
            20                  25                  30          


Asn Lys Phe Ala Ala Ile Cys Thr His Leu Glu Val Cys Phe Met Tyr 
        35                  40                  45              


Ser Asp Phe His Phe Ile Asn Glu Gln Gly Glu Ser Ile Ile Val Glu 
    50                  55                  60                  


Leu Gly Asp Pro Asn Ala Leu Leu Lys His Arg Phe Glu Ile Ile Glu 
65                  70                  75                  80  


Gly Arg Asp Arg Thr Met Ala Trp Thr Val Val Asn Ser Ile Cys Asn 
                85                  90                  95      


Thr Thr Gly Ala Glu Lys Pro Lys Phe Leu Pro Asp Leu Tyr Asp Tyr 
            100                 105                 110         


Lys Glu Asn Arg Phe Ile Glu Ile Gly Val Thr Arg Arg Glu Val His 
        115                 120                 125             


Ile Tyr Tyr Leu Glu Lys Ala Asn Lys Ile Lys Ser Glu Lys Thr His 
    130                 135                 140                 


Ile His Ile Phe Ser Phe Thr Gly Glu Glu Met Ala Thr Lys Ala Asp 
145                 150                 155                 160 


Tyr Thr Leu Asp Glu Glu Ser Arg Ala Arg Ile Lys Thr Arg Leu Phe 
                165                 170                 175     


Thr Ile Arg Gln Glu Met Ala Ser Arg Gly Leu Trp Asp Ser Phe Arg 
            180                 185                 190         


Gln Ser Glu Arg Gly Glu Glu Thr Ile Glu Glu Arg Phe Glu Ile Thr 
        195                 200                 205             


Gly Thr Met Arg Lys Leu Ala Asp Gln Ser Leu Pro Pro Asn Phe Ser 
    210                 215                 220                 


Ser Leu Glu Asn Phe Arg Ala Tyr Val Asp Gly Phe Glu Pro Asn Gly 
225                 230                 235                 240 


Tyr Ile Glu Gly Lys Leu Ser Gln Met Ser Lys Glu Val Asn Ala Arg 
                245                 250                 255     


Ile Glu Pro Phe Leu Lys Thr Thr Pro Arg Pro Leu Arg Leu Pro Asn 
            260                 265                 270         


Gly Pro Pro Cys Ser Gln Arg Ser Lys Phe Leu Leu Met Asp Ala Leu 
        275                 280                 285             


Lys Leu Ser Ile Glu Asp Pro Ser His Glu Gly Glu Gly Ile Pro Leu 
    290                 295                 300                 


Tyr Asp Ala Ile Lys Cys Met Arg Thr Phe Phe Gly Trp Lys Glu Pro 
305                 310                 315                 320 


Asn Val Val Lys Pro His Glu Lys Gly Ile Asn Pro Asn Tyr Leu Leu 
                325                 330                 335     


Ser Trp Lys Gln Val Leu Ala Glu Leu Gln Asp Ile Glu Asn Glu Glu 
            340                 345                 350         


Lys Ile Pro Lys Thr Lys Asn Met Lys Lys Thr Ser Gln Leu Lys Trp 
        355                 360                 365             


Ala Leu Gly Glu Asn Met Ala Pro Glu Lys Val Asp Phe Asp Asp Cys 
    370                 375                 380                 


Lys Asp Val Gly Asp Leu Lys Gln Tyr Asp Ser Asp Glu Pro Glu Leu 
385                 390                 395                 400 


Arg Ser Leu Ala Ser Trp Ile Gln Asn Glu Phe Asn Lys Ala Cys Glu 
                405                 410                 415     


Leu Thr Asp Ser Ser Trp Ile Glu Leu Asp Glu Ile Gly Glu Asp Val 
            420                 425                 430         


Ala Pro Ile Glu His Ile Ala Ser Met Arg Arg Asn Tyr Phe Thr Ser 
        435                 440                 445             


Glu Val Ser His Cys Arg Ala Thr Glu Tyr Ile Met Lys Gly Val Tyr 
    450                 455                 460                 


Ile Asn Thr Ala Leu Leu Asn Ala Ser Cys Ala Ala Met Asp Asp Phe 
465                 470                 475                 480 


Gln Leu Ile Pro Met Ile Ser Lys Cys Arg Thr Lys Glu Gly Arg Arg 
                485                 490                 495     


Lys Thr Asn Leu Tyr Gly Phe Ile Ile Lys Gly Arg Ser His Leu Arg 
            500                 505                 510         


Asn Asp Thr Asp Val Val Asn Phe Val Ser Met Glu Phe Ser Leu Thr 
        515                 520                 525             


Asp Pro Arg Leu Glu Pro His Lys Trp Glu Lys Tyr Cys Val Leu Glu 
    530                 535                 540                 


Ile Gly Asp Met Leu Ile Arg Ser Ala Ile Gly Gln Val Ser Arg Pro 
545                 550                 555                 560 


Met Phe Leu Tyr Val Arg Thr Asn Gly Thr Ser Lys Ile Lys Met Lys 
                565                 570                 575     


Trp Gly Met Glu Met Arg Arg Cys Leu Leu Gln Ser Leu Gln Gln Ile 
            580                 585                 590         


Glu Ser Met Ile Glu Ala Glu Ser Ser Val Lys Glu Lys Asp Met Thr 
        595                 600                 605             


Lys Glu Phe Phe Glu Asn Lys Ser Glu Thr Trp Pro Ile Gly Glu Ser 
    610                 615                 620                 


Pro Lys Gly Val Glu Glu Ser Ser Ile Gly Lys Val Cys Arg Thr Leu 
625                 630                 635                 640 


Leu Ala Lys Ser Val Phe Asn Ser Leu Tyr Ala Ser Pro Gln Leu Glu 
                645                 650                 655     


Gly Phe Ser Ala Glu Ser Arg Lys Leu Leu Leu Ile Val Gln Ala Leu 
            660                 665                 670         


Arg Asp Asn Leu Glu Pro Gly Thr Phe Asp Leu Gly Gly Leu Tyr Glu 
        675                 680                 685             


Ala Ile Glu Glu Cys Leu Ile Asn Asp Pro Trp Val Leu Leu Asn Ala 
    690                 695                 700                 


Ser Trp Phe Asn Ser Phe Leu Thr His Ala Leu Ser 
705                 710                 715     


<210>  24
<211>  1764
<212>  DNA
<213>  Influenza A virus

<400>  24
agcaaaagca ggggaaaaca aaagcaacaa aaatgaaggc aatactagta gttctgctat       60

atacatttgt aaccgcaaat gcagacacat tatgtatagg ttatcatgcg aacaattcaa      120

cagacactgt agacacagta ctagaaaaga atgtaacagt aacacactct gttaaccttc      180

tagaagacaa gcataacggg aaactatgca aactaagagg ggtagcccca ttgcatttgg      240

gtaaatgtaa cattgctggc tggatcctgg gaaatccaga gtgtgaatca ctctccacag      300

caagctcatg gtcctacatt gtggaaacac ctagttcaga caatggaacg tgttacccag      360

gagatttcat cgattatgag gagctaagag agcaattgag ctcagtgtca tcatttgaaa      420

ggtttgagat attccccaag acaagttcat ggcccaatca tgactcgaac aaaggtgtaa      480

cggcagcatg tcctcatgct ggagcaaaaa gcttctacaa aaatttaata tggctagtta      540

aaaaaggaaa ttcataccca aagctcagca aatcctacat taatgataaa gggaaagaag      600

tccttgtgct atggggcatt caccatccat ctactagtgc tgaccaacaa agtctctatc      660

agaatgcaga tgcatatgtt tttgtggggt catcaagata cagcaagaag ttcaagccgg      720

aaatagcaat aagacccaaa gtgagggatc gagaagggag aatgaactat tactggacac      780

tagtagagcc gggagacaaa ataacattcg aagcaactgg aaatctagtg gtaccgaaat      840

atgcattcgc aatggaaaga aatgctggat ctggtattat catttcagat acaccagtcc      900

acgattgcaa tacaacttgt caaacaccca agggtgctat aaacaccagc ctcccatttc      960

agaatataca tccgatcaca attggaaaat gtccaaaata tgtaaaaagc acaaaattga     1020

gactggccac aggattgagg aatatcccgt ctattcaatc tagaggccta tttggggcca     1080

ttgccggttt cattgaaggg gggtggacag ggatggtaga tggatggtac ggttatcacc     1140

atcaaaatga gcaggggtca ggatatgcag ccgacctgaa gagcacacag aatgccattg     1200

acgagattac taacaaagta aattctgtta ttgaaaagat gaatacacag ttcacagcag     1260

taggtaaaga gttcaaccac ctagaaaaaa gaatagagaa tttaaataaa aaagttgatg     1320

atggtttcct ggacatttgg acttacaatg ccgaactgtt ggttctattg gaaaatgaaa     1380

gaactttgga ctaccacgat tcaaatgtga agaacttata tgaaaaggta agaagccagc     1440

taaaaaacaa tgccaaggaa attggaaacg gctgctttga attttaccac aaatgcgata     1500

acacgtgcat ggaaagtgtc aaaaatggga cttatgacta cccaaaatac tcagaggaag     1560

caaaattaaa cagagaagaa atagatgggg taaagctgga atcaacaagg atttaccaga     1620

ttttggcgat ctattcaact gtcgccagtt cattggtact ggtagtctcc ctgggggcaa     1680

tcagtttctg gatgtgctct aatgggtctc tacagtgtag aatatgtatt taacattagg     1740

atttcagaag catgagaaaa acac                                            1764


<210>  25
<211>  566
<212>  PRT
<213>  Influenza A virus

<400>  25

Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Val Thr Ala Asn 
1               5                   10                  15      


Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr 
            20                  25                  30          


Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn 
        35                  40                  45              


Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val 
    50                  55                  60                  


Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly 
65                  70                  75                  80  


Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile 
                85                  90                  95      


Val Glu Thr Pro Ser Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe 
            100                 105                 110         


Ile Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe 
        115                 120                 125             


Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp 
    130                 135                 140                 


Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser 
145                 150                 155                 160 


Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro 
                165                 170                 175     


Lys Leu Ser Lys Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val 
            180                 185                 190         


Leu Trp Gly Ile His His Pro Ser Thr Ser Ala Asp Gln Gln Ser Leu 
        195                 200                 205             


Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Ser Ser Arg Tyr Ser 
    210                 215                 220                 


Lys Lys Phe Lys Pro Glu Ile Ala Ile Arg Pro Lys Val Arg Asp Arg 
225                 230                 235                 240 


Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys 
                245                 250                 255     


Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Lys Tyr Ala Phe 
            260                 265                 270         


Ala Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro 
        275                 280                 285             


Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Lys Gly Ala Ile Asn 
    290                 295                 300                 


Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys 
305                 310                 315                 320 


Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg 
                325                 330                 335     


Asn Ile Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly 
            340                 345                 350         


Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr 
        355                 360                 365             


His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser 
    370                 375                 380                 


Thr Gln Asn Ala Ile Asp Glu Ile Thr Asn Lys Val Asn Ser Val Ile 
385                 390                 395                 400 


Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His 
                405                 410                 415     


Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe 
            420                 425                 430         


Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn 
        435                 440                 445             


Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu 
    450                 455                 460                 


Lys Val Arg Ser Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly 
465                 470                 475                 480 


Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val 
                485                 490                 495     


Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu 
            500                 505                 510         


Asn Arg Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr 
        515                 520                 525             


Gln Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val 
    530                 535                 540                 


Val Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu 
545                 550                 555                 560 


Gln Cys Arg Ile Cys Ile 
                565     


<210>  26
<211>  1443
<212>  DNA
<213>  Influenza A virus

<400>  26
agcaaaagca ggagtttaaa atgaatccaa accaaaagat aataaccatt ggttcggtct       60

gtatgacaat tggaatggct aacttaatat tacaaattgg aaacataatc tcaatatgga      120

ttagccactc aattcaactt gggaatcaaa atcagattga aacatgcaat caaagcgtca      180

ttacttatga aaacaacact tgggtaaatc agacatatgt taacatcagc aacaccaact      240

ttgctgctgg acagtcagtg gtttccgtga aattagcggg caattcctct ctctgccctg      300

ttagtggatg ggctatatac agtaaagaca acagtgtaag aatcggttcc aagggggatg      360

tgtttgtcat aagggaacca ttcatatcat gctccccctt ggaatgcaga accttcttct      420

tgactcaagg ggccttgcta aatgacaaac attccaatgg aaccattaaa gacaggagcc      480

catatcgaac cctaatgagc tgtcctattg gtgaagttcc ctctccatac aactcaagat      540

ttgagtcagt cgcttggtca gcaagtgctt gtcatgatgg catcaattgg ctaacaattg      600

gaatttctgg cccagacaat ggggcagtgg ctgtgttaaa gtacaacggc ataataacag      660

acactatcaa gagttggaga aacaatatat tgagaacaca agagtctgaa tgtgcatgtg      720

taaatggttc ttgctttact gtaatgaccg atggaccaag taatggacag gcctcataca      780

agatcttcag aatagaaaag ggaaagatag tcaaatcagt cgaaatgaat gcccctaatt      840

atcactatga ggaatgctcc tgttatcctg attctagtga aatcacatgt gtgtgcaggg      900

ataactggca tggctcgaat cgaccgtggg tgtctttcaa ccagaatctg gaatatcaga      960

taggatacat atgcagtggg attttcggag acaatccacg ccctaatgat aagacaggca     1020

gttgtggtcc agtatcgtct aatggagcaa atggagtaaa agggttttca tacaaatatg     1080

gcaatggtgt ttggataggg agaactaaaa gcattagttc aagaaacggt tttgagatga     1140

tttgggatcc gaacggatgg actgggacag acaataactt ctcaataaag caagatatcg     1200

taggaataaa tgagtggtca ggatatagcg ggagttttgt tcagcatcca gaactaacag     1260

ggctggattg tataagacct tgcttctggg ttgaactaat cagagggcga cccaaagaga     1320

acacaatctg gactagcggg agcagcatat ccttttgtgg tgtaaacagt gacactgtgg     1380

gttggtcttg gccagacggt gctgagttgc catttaccat tgacaagtaa ttgttcaaaa     1440

aac                                                                   1443


<210>  27
<211>  469
<212>  PRT
<213>  Influenza A virus

<400>  27

Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Cys Met Thr 
1               5                   10                  15      


Ile Gly Met Ala Asn Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile 
            20                  25                  30          


Trp Ile Ser His Ser Ile Gln Leu Gly Asn Gln Asn Gln Ile Glu Thr 
        35                  40                  45              


Cys Asn Gln Ser Val Ile Thr Tyr Glu Asn Asn Thr Trp Val Asn Gln 
    50                  55                  60                  


Thr Tyr Val Asn Ile Ser Asn Thr Asn Phe Ala Ala Gly Gln Ser Val 
65                  70                  75                  80  


Val Ser Val Lys Leu Ala Gly Asn Ser Ser Leu Cys Pro Val Ser Gly 
                85                  90                  95      


Trp Ala Ile Tyr Ser Lys Asp Asn Ser Val Arg Ile Gly Ser Lys Gly 
            100                 105                 110         


Asp Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser Pro Leu Glu 
        115                 120                 125             


Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys His 
    130                 135                 140                 


Ser Asn Gly Thr Ile Lys Asp Arg Ser Pro Tyr Arg Thr Leu Met Ser 
145                 150                 155                 160 


Cys Pro Ile Gly Glu Val Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser 
                165                 170                 175     


Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Ile Asn Trp Leu Thr 
            180                 185                 190         


Ile Gly Ile Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu Lys Tyr 
        195                 200                 205             


Asn Gly Ile Ile Thr Asp Thr Ile Lys Ser Trp Arg Asn Asn Ile Leu 
    210                 215                 220                 


Arg Thr Gln Glu Ser Glu Cys Ala Cys Val Asn Gly Ser Cys Phe Thr 
225                 230                 235                 240 


Val Met Thr Asp Gly Pro Ser Asn Gly Gln Ala Ser Tyr Lys Ile Phe 
                245                 250                 255     


Arg Ile Glu Lys Gly Lys Ile Val Lys Ser Val Glu Met Asn Ala Pro 
            260                 265                 270         


Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Ser Ser Glu Ile 
        275                 280                 285             


Thr Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro Trp Val 
    290                 295                 300                 


Ser Phe Asn Gln Asn Leu Glu Tyr Gln Ile Gly Tyr Ile Cys Ser Gly 
305                 310                 315                 320 


Ile Phe Gly Asp Asn Pro Arg Pro Asn Asp Lys Thr Gly Ser Cys Gly 
                325                 330                 335     


Pro Val Ser Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Tyr Lys 
            340                 345                 350         


Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Ser Ser Arg 
        355                 360                 365             


Asn Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Gly Thr Asp 
    370                 375                 380                 


Asn Asn Phe Ser Ile Lys Gln Asp Ile Val Gly Ile Asn Glu Trp Ser 
385                 390                 395                 400 


Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Leu Asp 
                405                 410                 415     


Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro Lys 
            420                 425                 430         


Glu Asn Thr Ile Trp Thr Ser Gly Ser Ser Ile Ser Phe Cys Gly Val 
        435                 440                 445             


Asn Ser Asp Thr Val Gly Trp Ser Trp Pro Asp Gly Ala Glu Leu Pro 
    450                 455                 460                 


Phe Thr Ile Asp Lys 
465                 


