                         SEQUENCE LISTING

<110>  Roche Diagnostics GmbH
       Roche Diabetes Care, Inc.
       Roche Diabetes Care GmbH
       Roche Diagnostics Operations, Inc.
       F. Hoffmann-La Roche AG
 
<120>  Mutant 3-Hydroxybutyrate Dehydrogenase from Rhodobacter 
       sphaeroides as well as methods and uses involving the same

<130>  R69993PC

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  257
<212>  PRT
<213>  Rhodobacter sphaeroides

<400>  1

Met Asp Leu Asn Gly Lys Arg Ala Ile Val Thr Gly Ser Asn Ser Gly 
1               5                   10                  15      


Ile Gly Leu Gly Cys Ala Glu Glu Leu Ala Arg Ala Gly Ala Glu Val 
            20                  25                  30          


Val Ile Asn Ser Phe Thr Asp Arg Asp Glu Asp His Ala Leu Ala Glu 
        35                  40                  45              


Lys Ile Gly Arg Glu His Gly Val Ser Cys Arg Tyr Ile Ala Ala Asp 
    50                  55                  60                  


Met Ser Asp Gly Glu Ala Cys Arg Ala Leu Ile Glu Thr Ala Gly Gly 
65                  70                  75                  80  


Cys Asp Ile Leu Val Asn Asn Ala Gly Ile Gln His Val Ser Ser Ile 
                85                  90                  95      


Glu Glu Phe Pro Val Gly Lys Trp Asn Ala Ile Leu Ala Ile Asn Leu 
            100                 105                 110         


Ser Ser Ala Phe His Thr Thr Ala Ala Ala Leu Pro Gly Met Arg Ala 
        115                 120                 125             


Lys Gly Trp Gly Arg Ile Val Asn Ile Ala Ser Ala His Gly Leu Thr 
    130                 135                 140                 


Ala Ser Pro Tyr Lys Ser Ala Tyr Val Ala Ala Lys His Gly Val Val 
145                 150                 155                 160 


Gly Phe Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Lys Gly Ile Thr 
                165                 170                 175     


Cys Asn Ala Ile Cys Pro Gly Tyr Val Leu Thr Pro Leu Val Glu Ala 
            180                 185                 190         


Gln Ile Pro Asp Gln Met Lys Ala His Asp Met Asp Arg Glu Thr Val 
        195                 200                 205             


Ile Arg Glu Val Met Leu Asp Arg Gln Pro Ser Arg Gln Phe Ala Thr 
    210                 215                 220                 


Thr Gly Gln Ile Gly Gly Thr Val Val Phe Leu Cys Ser Gly Ala Ala 
225                 230                 235                 240 


Asp Gln Ile Thr Gly Thr Thr Ile Ser Val Asp Gly Gly Trp Thr Ala 
                245                 250                 255     


Leu 
    


<210>  2
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 3-HBDH

<400>  2

Met Asp Leu Asn Gly Lys Arg Ala Ile Val Thr Gly Ser Asn Ser Gly 
1               5                   10                  15      


Ile Gly Leu Gly Cys Ala Glu Glu Leu Ala Arg Ala Gly Ala Glu Val 
            20                  25                  30          


Val Ile Asn Ser Phe Thr Asp Arg Asp Glu Asp His Ala Leu Ala Glu 
        35                  40                  45              


Lys Ile Gly Arg Glu His Gly Val Ser Cys Arg Tyr Ile Ala Ala Asp 
    50                  55                  60                  


Met Ser Asp Gly Glu Ala Cys Arg Ala Leu Ile Glu Thr Ala Gly Gly 
65                  70                  75                  80  


Cys Asp Ile Leu Val Asn Asn Ala Gly Ile Gln His Val Ser Ser Ile 
                85                  90                  95      


Glu Glu Phe Pro Val Gly Lys Trp Asn Ala Ile Leu Ala Ile Asn Leu 
            100                 105                 110         


Ser Ser Ala Phe His Thr Thr Ala Ala Ala Leu Pro Phe Met Arg Ala 
        115                 120                 125             


Lys Gly Trp Gly Arg Ile Val Asn Ile Ala Ser Ala His Gly Leu Thr 
    130                 135                 140                 


Ala Ser Pro Tyr Lys Ser Ala Tyr Val Ala Ala Lys His Gly Val Val 
145                 150                 155                 160 


Gly Phe Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Lys Gly Ile Thr 
                165                 170                 175     


Cys Asn Ala Ile Cys Pro Gly Tyr Val Leu Thr Pro Leu Val Glu Ala 
            180                 185                 190         


Gln Ile Pro Asp Gln Met Lys Ala His Asp Met Asp Arg Glu Thr Val 
        195                 200                 205             


Ile Arg Glu Val Met Leu Asp Arg Gln Pro Ser Arg Gln Phe Ala Thr 
    210                 215                 220                 


Thr Gly Gln Ile Gly Gly Thr Val Val Phe Leu Cys Ser Gly Ala Ala 
225                 230                 235                 240 


Asp Gln Ile Thr Gly Thr Thr Ile Ser Val Asp Gly Gly Trp Thr Ala 
                245                 250                 255     


Leu 
    


<210>  3
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 3-HBDH

<400>  3

Met Asp Leu Asn Gly Lys Arg Ala Ile Val Thr Gly Ser Asn Ser Gly 
1               5                   10                  15      


Ile Gly Leu Gly Cys Ala Glu Glu Leu Ala Arg Ala Gly Ala Glu Val 
            20                  25                  30          


Val Ile Asn Ser Phe Thr Asp Arg Asp Glu Asp His Ala Leu Ala Glu 
        35                  40                  45              


Lys Ile Gly Arg Glu His Gly Val Ser Cys Arg Tyr Ile Ala Ala Asp 
    50                  55                  60                  


Met Ser Asp Gly Glu Ala Cys Arg Ala Leu Ile Glu Thr Ala Gly Gly 
65                  70                  75                  80  


Cys Asp Ile Leu Val Asn Asn Ala Gly Ile Gln His Val Ser Ser Ile 
                85                  90                  95      


Glu Glu Phe Pro Val Gly Lys Trp Asn Ala Ile Leu Ala Ile Asn Leu 
            100                 105                 110         


Ser Ser Ala Phe His Thr Thr Ala Ala Ala Leu Pro Gly Met Arg Ala 
        115                 120                 125             


Lys Gly Trp Gly Arg Ile Val Asn Ile Ala Ser Ala His Gly Leu Arg 
    130                 135                 140                 


Ala Ser Pro Tyr Lys Ser Ala Tyr Val Ala Ala Lys His Gly Val Val 
145                 150                 155                 160 


Gly Phe Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Lys Gly Ile Thr 
                165                 170                 175     


Cys Asn Ala Ile Cys Pro Gly Tyr Val Leu Thr Pro Leu Val Glu Ala 
            180                 185                 190         


Gln Ile Pro Asp Gln Met Lys Ala His Asp Met Asp Arg Glu Thr Val 
        195                 200                 205             


Ile Arg Glu Val Met Leu Asp Arg Gln Pro Ser Arg Gln Phe Ala Thr 
    210                 215                 220                 


Thr Gly Gln Ile Gly Gly Thr Val Val Phe Leu Cys Ser Gly Ala Ala 
225                 230                 235                 240 


Asp Gln Ile Thr Gly Thr Thr Ile Ser Val Asp Gly Gly Trp Thr Ala 
                245                 250                 255     


Leu 
    


<210>  4
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 3-HBDH

<400>  4

Met Asp Leu Asn Gly Lys Arg Ala Ile Val Thr Gly Ser Asn Ser Gly 
1               5                   10                  15      


Ile Gly Leu Gly Cys Ala Glu Glu Leu Ala Arg Ala Gly Ala Glu Val 
            20                  25                  30          


Val Ile Asn Ser Phe Thr Asp Arg Asp Glu Asp His Ala Leu Ala Glu 
        35                  40                  45              


Lys Ile Gly Arg Glu His Gly Val Ser Cys Arg Tyr Ile Ala Ala Asp 
    50                  55                  60                  


Met Ser Asp Gly Glu Ala Cys Arg Ala Leu Ile Glu Thr Ala Gly Gly 
65                  70                  75                  80  


Cys Asp Ile Leu Val Asn Asn Ala Gly Ile Gln His Val Ser Ser Ile 
                85                  90                  95      


Glu Glu Phe Pro Val Gly Lys Trp Asn Ala Ile Leu Ala Ile Asn Leu 
            100                 105                 110         


Ser Ser Ala Phe His Thr Thr Ala Ala Ala Leu Pro Gly Met Arg Ala 
        115                 120                 125             


Lys Gly Trp Gly Arg Ile Val Asn Ile Ala Ser Ala His Gly Leu Thr 
    130                 135                 140                 


Ala Ser Pro Tyr Lys Ser Ala Tyr Val Ala Ala Lys His Gly Val Val 
145                 150                 155                 160 


Gly Phe Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Lys Gly Ile Thr 
                165                 170                 175     


Cys Asn Ala Ile Cys Pro Gly Tyr Val Leu Thr Pro Leu Val Glu Ala 
            180                 185                 190         


Gln Ile Pro Asp Gln Met Lys Ala His Asp Met Asp Arg Glu Thr Val 
        195                 200                 205             


Ile Arg Glu Val Met Leu Asp Arg Gln Pro Ser Arg Gln Phe Ala Thr 
    210                 215                 220                 


Thr Gly Gln Ile Gly Gly Thr Cys Val Phe Leu Cys Ser Gly Ala Ala 
225                 230                 235                 240 


Asp Gln Ile Thr Gly Thr Thr Ile Ser Val Asp Gly Gly Trp Thr Ala 
                245                 250                 255     


Leu 
    


<210>  5
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 3-HBDH

<400>  5

Met Asp Leu Asn Gly Lys Arg Ala Ile Val Thr Gly Ser Asn Ser Gly 
1               5                   10                  15      


Ile Gly Leu Gly Cys Ala Glu Glu Leu Ala Arg Ala Gly Ala Glu Val 
            20                  25                  30          


Val Ile Asn Ser Phe Thr Asp Arg Asp Glu Asp His Ala Leu Ala Glu 
        35                  40                  45              


Lys Ile Gly Arg Glu His Gly Val Ser Cys Arg Tyr Ile Ala Ala Asp 
    50                  55                  60                  


Met Ser Asp Gly Glu Ala Cys Arg Ala Leu Ile Glu Thr Ala Gly Gly 
65                  70                  75                  80  


Cys Asp Ile Leu Val Asn Asn Ala Gly Ile Gln His Val Ser Ser Ile 
                85                  90                  95      


Glu Glu Phe Pro Val Gly Lys Trp Asn Ala Ile Leu Ala Ile Asn Leu 
            100                 105                 110         


Ser Ser Ala Phe His Thr Thr Ala Ala Ala Leu Pro Gly Met Arg Ala 
        115                 120                 125             


Lys Gly Trp Gly Arg Ile Val Asn Ile Ala Ser Ala His Gly Leu Thr 
    130                 135                 140                 


Ala Ser Pro Tyr Lys Ser Ala Tyr Val Ala Ala Lys His Gly Val Val 
145                 150                 155                 160 


Gly Phe Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Lys Gly Ile Thr 
                165                 170                 175     


Cys Asn Ala Ile Cys Pro Gly Tyr Val Leu Thr Pro Leu Val Glu Ala 
            180                 185                 190         


Gln Ile Pro Asp Gln Met Lys Ala His Asp Met Asp Arg Glu Thr Val 
        195                 200                 205             


Ile Arg Glu Val Met Leu Asp Arg Gln Pro Ser Arg Gln Phe Ala Thr 
    210                 215                 220                 


Thr Gly Gln Ile Gly Gly Thr Val Val Phe Leu Cys Ser Gly Ala Ala 
225                 230                 235                 240 


Asp Gln Ile Thr Gly Thr Thr Ile Ser Met Asp Gly Gly Trp Thr Ala 
                245                 250                 255     


Leu 
    


<210>  6
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 3-HBDH

<400>  6

Met Asp Leu Asn Gly Lys Arg Ala Ile Val Thr Gly Ser Asn Ser Gly 
1               5                   10                  15      


Ile Gly Leu Gly Cys Ala Glu Glu Leu Ala Arg Ala Gly Ala Glu Val 
            20                  25                  30          


Val Ile Asn Ser Phe Thr Asp Arg Asp Glu Asp His Ala Leu Ala Glu 
        35                  40                  45              


Lys Ile Gly Arg Glu His Gly Val Ser Cys Arg Tyr Ile Ala Ala Asp 
    50                  55                  60                  


Met Ser Asp Gly Glu Ala Cys Arg Ala Leu Ile Glu Thr Ala Gly Gly 
65                  70                  75                  80  


Cys Asp Ile Leu Val Asn Asn Ala Gly Ile Gln His Val Ser Ser Ile 
                85                  90                  95      


Glu Glu Phe Pro Val Gly Lys Trp Asn Ala Ile Leu Ala Ile Asn Leu 
            100                 105                 110         


Ser Ser Ala Phe His Thr Thr Ala Ala Ala Leu Pro Gly Met Arg Ala 
        115                 120                 125             


Lys Gly Trp Gly Arg Ile Val Asn Ile Ala Ser Ala His Gly Leu Thr 
    130                 135                 140                 


Ala Ser Pro Tyr Lys Ser Ala Tyr Val Ala Ala Lys His Gly Val Val 
145                 150                 155                 160 


Gly Phe Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Lys Gly Ile Thr 
                165                 170                 175     


Cys Asn Ala Ile Cys Pro Gly Tyr Val Leu Thr Pro Leu Val Glu Ala 
            180                 185                 190         


Gln Ile Pro Asp Gln Met Lys Ala His Asp Met Asp Arg Glu Thr Val 
        195                 200                 205             


Ile Arg Glu Val Met Leu Asp Arg Gln Pro Ser Arg Gln Phe Ala Thr 
    210                 215                 220                 


Thr Gly Gln Ile Gly Leu Thr Val Val Phe Leu Cys Ser Gly Ala Ala 
225                 230                 235                 240 


Asp Gln Ile Thr Gly Thr Thr Ile Ser Met Asp Gly Gly Trp Thr Ala 
                245                 250                 255     


Leu 
    


<210>  7
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 3-HBDH

<400>  7

Met Asp Leu Asn Gly Lys Arg Ala Ile Val Thr Gly Ser Asn Ser Gly 
1               5                   10                  15      


Ile Gly Leu Gly Cys Ala Glu Glu Leu Ala Arg Ala Gly Ala Glu Val 
            20                  25                  30          


Val Ile Asn Ser Tyr Thr Asp Arg Asp Glu Asp His Ala Leu Ala Glu 
        35                  40                  45              


Lys Ile Gly Arg Glu His Gly Val Ser Cys Arg Tyr Ile Ala Ala Asp 
    50                  55                  60                  


Met Ser Asp Gly Glu Ala Cys Arg Ala Leu Ile Glu Thr Ala Gly Gly 
65                  70                  75                  80  


Cys Asp Ile Leu Val Asn Asn Ala Gly Ile Gln His Val Ser Ser Ile 
                85                  90                  95      


Glu Glu Phe Pro Val Gly Lys Trp Asn Ala Ile Leu Ala Ile Asn Leu 
            100                 105                 110         


Ser Ser Ala Phe His Thr Thr Ala Ala Ala Leu Pro Gly Met Arg Ala 
        115                 120                 125             


Lys Gly Trp Gly Arg Ile Val Asn Ile Ala Ser Ala His Gly Leu Thr 
    130                 135                 140                 


Ala Ser Pro Tyr Lys Ser Ala Tyr Val Ala Ala Lys His Gly Val Val 
145                 150                 155                 160 


Gly Phe Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Lys Gly Ile Thr 
                165                 170                 175     


Cys Asn Ala Ile Cys Pro Gly Tyr Val Leu Thr Pro Leu Val Glu Ala 
            180                 185                 190         


Gln Ile Pro Asp Gln Met Lys Ala His Asp Met Asp Arg Glu Thr Val 
        195                 200                 205             


Ile Arg Glu Val Met Leu Asp Arg Gln Pro Ser Arg Gln Phe Ala Thr 
    210                 215                 220                 


Thr Gly Gln Ile Gly Gly Thr Val Val Phe Leu Cys Ser Gly Ala Ala 
225                 230                 235                 240 


Asp Gln Ile Thr Gly Thr Thr Ile Ser Met Asp Gly Gly Trp Thr Ala 
                245                 250                 255     


Leu 
    


<210>  8
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 3-HBDH

<400>  8

Met Asp Leu Asn Gly Lys Arg Ala Ile Val Thr Gly Ser Asn Ser Gly 
1               5                   10                  15      


Ile Gly Leu Gly Cys Ala Glu Glu Leu Ala Arg Ala Gly Ala Glu Val 
            20                  25                  30          


Val Ile Asn Ser Tyr Thr Asp Arg Asp Glu Asp His Ala Leu Ala Glu 
        35                  40                  45              


Lys Ile Gly Arg Glu His Gly Val Ser Cys Arg Tyr Ile Ala Ala Asp 
    50                  55                  60                  


Met Ser Asp Gly Glu Ala Cys Arg Ala Leu Ile Glu Thr Ala Gly Gly 
65                  70                  75                  80  


Cys Asp Ile Leu Val Asn Asn Ala Gly Ile Gln His Val Ser Ser Ile 
                85                  90                  95      


Glu Glu Phe Pro Val Gly Lys Trp Asn Ala Ile Leu Ala Ile Asn Leu 
            100                 105                 110         


Ser Ser Ala Phe His Thr Thr Ala Ala Ala Leu Pro Gly Met Arg Ala 
        115                 120                 125             


Lys Gly Trp Gly Arg Ile Val Asn Ile Ala Ser Ala His Gly Leu Thr 
    130                 135                 140                 


Ala Ser Pro Tyr Lys Ser Ala Tyr Val Ala Ala Lys His Gly Val Val 
145                 150                 155                 160 


Gly Phe Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Lys Gly Ile Thr 
                165                 170                 175     


Cys Asn Ala Ile Cys Pro Gly Tyr Val Leu Thr Pro Leu Val Glu Ala 
            180                 185                 190         


Gln Ile Pro Asp Gln Met Lys Ala His Asp Met Asp Arg Glu Thr Val 
        195                 200                 205             


Ile Arg Glu Val Met Leu Asp Arg Gln Pro Ser Arg Gln Phe Ala Thr 
    210                 215                 220                 


Thr Gly Gln Ile Gly Leu Thr Val Val Phe Leu Cys Ser Gly Ala Ala 
225                 230                 235                 240 


Asp Gln Ile Thr Gly Thr Thr Ile Ser Met Asp Gly Gly Trp Thr Ala 
                245                 250                 255     


Leu 
    


<210>  9
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 3-HBDH

<400>  9

Met Asp Leu Asn Gly Lys Arg Ala Ile Val Thr Gly Ser Asn Ser Gly 
1               5                   10                  15      


Ile Gly Leu Gly Cys Ala Glu Glu Leu Ala Arg Ala Gly Ala Glu Val 
            20                  25                  30          


Val Ile Asn Ser Phe Thr Asp Arg Asp Glu Asp His Ala Leu Ala Glu 
        35                  40                  45              


Lys Ile Gly Arg Glu His Gly Val Ser Cys Arg Tyr Ile Ala Ala Asp 
    50                  55                  60                  


Met Ser Asp Gly Glu Ala Cys Arg Ala Leu Ile Glu Thr Ala Gly Gly 
65                  70                  75                  80  


Cys Asp Ile Leu Val Asn Asn Ala Gly Ile Gln His Val Ser Ser Ile 
                85                  90                  95      


Glu Glu Phe Pro Val Gly Lys Trp Asn Ala Ile Leu Ala Ile Asn Leu 
            100                 105                 110         


Ser Ser Ala Phe His Thr Thr Ala Ala Ala Leu Pro Gly Met Arg Ala 
        115                 120                 125             


Lys Gly Trp Gly Arg Ile Val Asn Ile Ala Ser Ala His Gly Leu Thr 
    130                 135                 140                 


Ala Ser Pro Tyr Lys Ser Ala Tyr Val Ala Ala Lys His Gly Val Val 
145                 150                 155                 160 


Gly Phe Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Lys Gly Ile Thr 
                165                 170                 175     


Cys Asn Ala Ile Cys Pro Gly Tyr Val Leu Thr Pro Leu Val Glu Ala 
            180                 185                 190         


Gln Ile Pro Asp Gln Met Lys Ala His Gly Met Asp Arg Glu Thr Val 
        195                 200                 205             


Ile Arg Glu Val Met Leu Asp Arg Gln Pro Ser Arg Gln Phe Ala Thr 
    210                 215                 220                 


Thr Gly Gln Ile Gly Leu Thr Val Val Phe Leu Cys Ser Gly Ala Ala 
225                 230                 235                 240 


Asp Gln Ile Thr Gly Thr Thr Ile Ser Met Asp Gly Gly Trp Thr Ala 
                245                 250                 255     


Leu 
    


<210>  10
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 3-HBDH

<400>  10

Met Asp Leu Asn Gly Lys Arg Ala Ile Val Thr Gly Ser Asn Ser Gly 
1               5                   10                  15      


Ile Gly Leu Gly Cys Ala Glu Glu Leu Ala Arg Ala Gly Ala Glu Val 
            20                  25                  30          


Val Ile Asn Ser Tyr Thr Asp Arg Asp Glu Asp His Ala Leu Ala Glu 
        35                  40                  45              


Lys Ile Gly Arg Glu His Gly Val Ser Cys Arg Tyr Ile Ala Ala Asp 
    50                  55                  60                  


Met Ser Asp Gly Glu Ala Cys Arg Ala Leu Ile Glu Thr Ala Gly Gly 
65                  70                  75                  80  


Cys Asp Ile Leu Val Asn Asn Ala Gly Ile Gln His Val Ser Ser Ile 
                85                  90                  95      


Glu Glu Phe Pro Val Gly Lys Trp Asn Ala Ile Leu Ala Ile Asn Leu 
            100                 105                 110         


Ser Ser Ala Phe His Thr Thr Ala Ala Ala Leu Pro Gly Met Arg Ala 
        115                 120                 125             


Lys Gly Trp Gly Arg Ile Val Asn Ile Ala Ser Ala His Gly Leu Thr 
    130                 135                 140                 


Gly Ser Pro Tyr Lys Ser Ala Tyr Val Ala Ala Lys His Gly Val Val 
145                 150                 155                 160 


Gly Phe Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Lys Gly Ile Thr 
                165                 170                 175     


Cys Asn Ala Ile Cys Pro Gly Tyr Val Leu Thr Pro Leu Val Glu Ala 
            180                 185                 190         


Gln Ile Pro Asp Gln Met Lys Ala His Asp Met Asp Arg Glu Thr Val 
        195                 200                 205             


Ile Arg Glu Val Met Leu Asp Arg Gln Pro Ser Arg Gln Phe Ala Thr 
    210                 215                 220                 


Thr Gly Gln Ile Gly Leu Thr Val Val Phe Leu Cys Ser Gly Ala Ala 
225                 230                 235                 240 


Asp Gln Ile Thr Gly Thr Thr Ile Ser Met Asp Gly Gly Trp Thr Ala 
                245                 250                 255     


Leu 
    


<210>  11
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 3-HBDH

<400>  11

Met Asp Leu Asn Gly Lys Arg Ala Ile Val Thr Gly Ser Asn Ser Gly 
1               5                   10                  15      


Ile Gly Leu Gly Cys Ala Glu Glu Leu Ala Arg Ala Gly Ala Glu Val 
            20                  25                  30          


Val Ile Asn Ser Tyr Thr Asp Arg Asp Glu Asp His Ala Leu Ala Glu 
        35                  40                  45              


Lys Ile Gly Arg Glu His Gly Val Ser Cys Arg Tyr Ile Ala Ala Asp 
    50                  55                  60                  


Met Ser Asp Gly Glu Ala Cys Arg Ala Leu Ile Glu Thr Ala Gly Gly 
65                  70                  75                  80  


Cys Asp Ile Leu Val Asn Asn Ala Gly Ile Gln His Val Ser Ser Ile 
                85                  90                  95      


Glu Glu Phe Pro Val Gly Lys Trp Asn Ala Ile Leu Ala Ile Asn Leu 
            100                 105                 110         


Ser Ser Ala Phe His Thr Thr Ala Ala Ala Leu Pro Gly Met Arg Ala 
        115                 120                 125             


Lys Gly Trp Gly Arg Ile Val Asn Ile Ala Ser Ala His Gly Leu Thr 
    130                 135                 140                 


Ala Ser Pro Tyr Lys Ser Ala Tyr Val Ala Ala Lys His Gly Val Val 
145                 150                 155                 160 


Gly Phe Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Lys Gly Ile Thr 
                165                 170                 175     


Cys Asn Ala Ile Cys Pro Gly Tyr Val Leu Thr Pro Leu Val Glu Ala 
            180                 185                 190         


Gln Ile Gln Asp Gln Met Lys Ala His Asp Met Asp Arg Glu Thr Val 
        195                 200                 205             


Ile Arg Glu Val Met Leu Asp Arg Gln Pro Ser Arg Gln Phe Ala Thr 
    210                 215                 220                 


Thr Gly Gln Ile Gly Leu Thr Val Val Phe Leu Cys Ser Gly Ala Ala 
225                 230                 235                 240 


Asp Gln Ile Thr Gly Thr Thr Ile Ser Met Asp Gly Gly Trp Thr Ala 
                245                 250                 255     


Leu 
    


