                         SEQUENCE LISTING

<110>  CureVac AG
 
<120>  Method for analyzing RNA

<130>  C 9933-WO/RN

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  1701
<212>  RNA
<213>  artificial

<220>
<223>  GC optimized sequence encoding H3N2

<400>  1
augaagacca ucaucgcccu gagcuacauc cucugccugg uguucgccca gaagcugccc       60

ggcaacgaca acuccaccgc gacgcucugc cuggggcacc acgccguccc gaacggcacc      120

aucgugaaga ccaucaccaa cgaccgcauc gaggugacga acgccaccga gcugguccag      180

aacagcucca ucggggagau cugcgacagc ccccaccaga uccucgacgg cgagaacugc      240

acccugaucg acgcccugcu cggggacccc cagugcgacg gcuuccagaa caagaagugg      300

gaccuguucg uggagcgguc caaggccuac agcaacugcu accccuacga cgugccggac      360

uacgcguccc ugcgcagccu cgucgccucc agcggcaccc uggaguucaa caacgagucc      420

uucaacugga acggggugac gcagaacggc accagcuccg ccugcauccg gcgcagcaac      480

aacuccuucu ucagccggcu gaacuggcuc acccaccuga acuucaagua ccccgcccug      540

aacgugacca ugcccaacaa cgagcaguuc gacaagcucu acaucugggg cguccaccac      600

cccgggacgg acaaggacca gaucuuccug uacgcgcagc cguccgggcg caucaccgug      660

agcaccaagc ggucccagca ggccgugauc cccaacaucg gcagccgccc ccggauccgc      720

aacaucccga gccggaucuc caucuacugg accaucguca agcccggcga cauccugcuc      780

aucaacagca cggggaaccu gaucgccccg cgcggcuacu ucaagauccg guccgggaag      840

agcuccauca ugcgcagcga cgcccccauc ggcaagugca aguccgagug caucaccccc      900

aacggcagca ucccgaacga caagcccuuc cagaacguga accggaucac cuacggggcc      960

ugcccccgcu acgugaagca guccacccug aagcucgcga cgggcaugcg gaacgucccc     1020

gagaagcaga cccgcgggau cuucggcgcc aucgccgggu ucaucgagaa cggcugggag     1080

ggcauggugg acggguggua cggcuuccgg caccagaaca gcgaggggcg cggccaggcc     1140

gccgaccuga aguccaccca ggcggccauc gaccagauca acgggaagcu gaaccggcuc     1200

aucggcaaga ccaacgagaa guuccaccag aucgagaagg aguucagcga gguggagggc     1260

cgcauccagg accuggagaa guacgucgag gacacgaaga ucgaccugug guccuacaac     1320

gccgagcucc ugguggcccu ggagaaccag cacaccaucg accucaccga cagcgagaug     1380

aacaagcugu ucgagaagac caagaagcag cugcgcgaga acgccgagga cauggggaac     1440

ggcugcuuca agaucuacca caagugcgac aacgcgugca ucggguccau ccggaacggc     1500

acguacgacc acgacgugua ccgcgacgag gcccucaaca accgguucca gaucaagggg     1560

gucgagcuga agagcggcua caaggacugg auccugugga ucuccuucgc caucagcugc     1620

uuccuccugu gcguggcccu gcucggcuuc aucauguggg ccugccagaa ggggaacauc     1680

cgcugcaaca ucugcaucug a                                               1701


<210>  2
<211>  1701
<212>  RNA
<213>  artificial

<220>
<223>  GC optimized sequence encoding H1N1

<400>  2
augaaggcca uccugguggu ccuccuguac accuucgcca ccgcgaacgc cgacacgcug       60

ugcaucggcu accacgccaa caacagcacc gacaccgugg acaccgugcu cgagaagaac      120

gucacgguga cccacuccgu gaaccugcug gaggacaagc acaacgggaa gcucugcaag      180

cugcggggcg ucgccccgcu gcaccucggg aagugcaaca ucgccggcug gauccugggg      240

aacccggagu gcgagagccu guccaccgcg agcuccugga gcuacaucgu ggagaccccc      300

uccagcgaca acggcacgug cuaccccggc gacuucaucg acuacgagga gcuccgcgag      360

cagcugucca gcguguccag cuucgagcgg uucgagaucu uccccaagac cuccagcugg      420

ccgaaccacg acuccgacaa gggggucacc gccgccugcc cccacgccgg cgcgaagagc      480

uucuacaaga accugaucug gcucgugaag aaggggaacu ccuaccccaa gcugagcaag      540

uccuacauca acgacaaggg caaggaggug cugguccucu gggggaucca ccaccccagc      600

accagcgccg accagcaguc ccuguaccag aacgccgacg ccuacguguu cgugggcagc      660

ucccgcuaca gcaagacguu caagccggag aucgccaucc ggcccaaggu ccgcgaccgg      720

gagggccgca ugaacuacua cuggacccug guggagcccg gggacaagau caccuucgag      780

gcgaccggca accucguggu cccccgguac gccuucgcca uggagcgcaa cgccgggucc      840

ggcaucauca ucagcgacac gccggugcac gacugcaaca ccaccugcca gacccccaag      900

ggcgccauca acacgucccu gcccuuccag aacauccacc ccaucaccau cgggaagugc      960

ccgaaguacg ugaagagcac caagcugcgg cucgcgaccg gccugcgcaa cauccccucc     1020

auccagagcc gggggcuguu cggcgccauc gccggguuca ucgagggcgg cuggacgggg     1080

auggucgacg gcugguacgg guaccaccac cagaacgagc agggcagcgg guacgccgcc     1140

gaccucaagu ccacgcagaa cgcgaucgac gagaucacca acaaggugaa cagcgucauc     1200

gagaagauga acacccaguu caccgccgug ggcaaggagu ucaaccaccu ggagaagcgg     1260

aucgagaacc ugaacaagaa ggucgacgac ggcuuccucg acaucuggac guacaacgcc     1320

gagcugcugg ugcuccugga gaacgagcgc acccuggacu accacgacuc caacgugaag     1380

aaccucuacg agaagguccg gagccagcug aagaacaacg ccaaggagau cgggaacggc     1440

ugcuucgagu ucuaccacaa gugcgacaac accugcaugg aguccgugaa gaacgggacc     1500

uacgacuacc ccaaguacag cgaggaggcc aagcugaacc gcgaggagau cgacggcgug     1560

aagcucgagu ccacgcggau cuaccagauc cuggcgaucu acagcaccgu cgccagcucc     1620

cuggugcucg uggucagccu gggggccauc uccuucugga ugugcagcaa cggcucccug     1680

cagugccgca ucugcaucug a                                               1701


<210>  3
<211>  1755
<212>  RNA
<213>  artificial

<220>
<223>  GC optimized sequence encoding HA influenza B

<400>  3
augaaggcca ucaucgugcu gcucaugguc gugaccagca acgccgaccg caucugcacc       60

ggcaucacgu ccagcaacuc cccgcacgug gucaagaccg cgacccaggg ggaggugaac      120

gugaccggcg ucaucccccu gacgaccacc cccaccaaga gcuacuucgc caaccugaag      180

gggacgaaga cccggggcaa gcucugcccc gacugccuga acugcaccga ccuggacgug      240

gcccucgggc gccccaugug cgugggcacc acgccguccg ccaaggccag cauccugcac      300

gagguccggc ccgugaccuc cggcugcuuc cccaucaugc acgaccgcac caagauccgg      360

cagcuggcga accuccugcg cggguacgag aacauccggc ugagcaccca gaacgugauc      420

gacgccgaga aggcccccgg cgggccguac cgccucggca cguccgggag cugccccaac      480

gccaccucca agagcggcuu cuucgcgacc auggccuggg ccguccccaa ggacaacaac      540

aagaacgcca ccaacccccu gacgguggag gugccguaca ucugcgccga gggcgaggac      600

cagaucaccg ucuggggguu ccacuccgac gacaagaccc agaugaagaa ccuguacggc      660

gacagcaacc cccagaaguu caccuccagc gcgaacgggg ugacgaccca cuacgugucc      720

cagaucggcg gcuuccccga ccagaccgag gacgggggcc ucccccagag cgggcggauc      780

gucguggacu acaugaugca gaagccgggc aagaccggga cgaucgugua ccagcgcggc      840

guccugcugc cccagaaggu guggugcgcc uccggccgga gcaaggugau caaggggagc      900

cucccccuga ucggcgaggc cgacugccug cacgagaagu acgggggccu caacaagucc      960

aagccguacu acaccgggga gcacgccaag gccaucggca acugccccau cugggucaag     1020

accccgcuga agcuggcgaa cggcaccaag uaccgcccgc ccgccaagcu ccugaaggag     1080

cggggguucu ucggcgccau cgccggguuc cuggagggcg ggugggaggg caugaucgcc     1140

ggcuggcacg gguacacgag ccacggcgcg cacggggugg ccgucgccgc cgaccucaag     1200

uccacccagg aggccaucaa caagaucacc aagaaccuga acagccuguc cgagcucgag     1260

gugaagaacc ugcagcgccu gagcggcgcg auggacgagc uccacaacga gauccuggag     1320

cuggacgaga agguggacga ccuccgggcc gacaccaucu ccagccagau cgagcuggcc     1380

guccugcucu ccaacgaggg caucaucaac agcgaggacg agcaccugcu ggcccucgag     1440

cgcaagcuga agaagaugcu ggggccgucc gcgguggaca ucggcaacgg gugcuucgag     1500

acgaagcaca agugcaacca gaccugccuc gaccggaucg ccgccggcac cuucaacgcc     1560

ggggaguuca gccugcccac cuucgacucc cugaacauca ccgccgcgag ccugaacgac     1620

gacggccucg acaaccacac gauccugcug uacuacucca ccgccgccag cucccucgcc     1680

gugacccuga ugcuggccau cuucaucguc uacaugguga gccgcgacaa cguguccugc     1740

agcaucugcc ucuga                                                      1755


<210>  4
<211>  1758
<212>  RNA
<213>  artificial

<220>
<223>  GC optimized sequence encoding HA influenza B Brisbane

<400>  4
augaaggcca ucaucgugcu gcucaugguc gugaccagca acgccgaccg gaucugcacc       60

ggcaucacgu ccagcaacuc cccgcacgug gucaagaccg cgacccaggg ggaggugaac      120

gugaccggcg ucaucccgcu gacgaccacc cccaccaaga gccacuucgc caaccugaag      180

gggacggaga cccgcggcaa gcucugcccc aagugccuga acugcaccga ccuggacgug      240

gcccucgggc ggcccaagug caccggcaag aucccguccg cccgcgugag cauccugcac      300

gagguccggc ccgugacguc cggcugcuuc cccaucaugc acgaccgcac caagauccgg      360

cagcugccca accuccugcg cggguacgag cacauccggc ugagcaccca caacgugauc      420

aacgccgaga acgcgccggg cgggcccuac aagaucggca ccuccgggag cugccccaac      480

aucacgaacg gcaacggcuu cuucgccacc auggccuggg ccguccccaa gaacgacaag      540

aacaagaccg cgaccaaccc gcucacgauc gaggugcccu acaucugcac cgagggggag      600

gaccagauca ccgugugggg cuuccacucc gacaacgaga cccagauggc caagcuguac      660

ggggacagca agccccagaa guucacgucc agcgccaacg gcgucaccac ccacuacgug      720

ucccagaucg gcggguuccc caaccagacc gaggacggcg ggcugccgca gagcggccgc      780

aucguggucg acuacauggu gcagaagucc gggaagacgg gcaccaucac cuaccagcgg      840

ggcauccucc ugccccagaa gguguggugc gccagcgggc gcuccaaggu caucaagggc      900

agccugcccc ucaucgggga ggccgacugc cugcacgaga aguacggcgg gcugaacaag      960

agcaagcccu acuacaccgg cgagcacgcg aaggccaucg gcaacugccc gaucugggug     1020

aagacgcccc ucaagcuggc caacgggacc aaguaccggc cccccgccaa gcugcucaag     1080

gagcgcggcu ucuucggggc caucgcgggc uuccuggagg gcggguggga gggcaugauc     1140

gccggguggc acggcuacac cucccacggg gcccacggcg uggccgucgc cgcggaccug     1200

aagagcaccc aggaggccau caacaagauc acgaagaacc ucaacucccu gagcgagcug     1260

gaggugaaga accuccagcg gcuguccggc gccauggacg agcugcacaa cgagauccuc     1320

gagcuggacg agaaggucga cgaccugcgc gccgacacca ucagcuccca gaucgagcuc     1380

gccgugcugc ugagcaacga ggggaucauc aacuccgagg acgagcaccu ccuggcgcug     1440

gagcggaagc ucaagaagau gcugggcccg agcgccgugg agaucgggaa cggcugcuuc     1500

gagaccaagc acaagugcaa ccagaccugc cuggaccgca ucgccgccgg gaccuucgac     1560

gcgggcgagu ucucccuccc cacguucgac agccugaaca ucaccgccgc cucccugaac     1620

gacgacggcc uggacaacca caccauccuc cuguacuaca gcaccgccgc cuccagccug     1680

gcggucacgc ucaugaucgc caucuucgug guguacaugg ucucccggga caacgugagc     1740

ugcuccaucu gccuguga                                                   1758


<210>  5
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  molecular beacon CU

<400>  5
cgaggtgttg aagagaagtt gcttgtcctc g                                      31


<210>  6
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  molecular beacon FR

<400>  6
cgaggttccg agctaactgt atttctcctc g                                      31


<210>  7
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  molecular beacon NU

<400>  7
cgaggaactg atgtcttgaa attaaccctc g                                      31


<210>  8
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  molecular beacon SP

<400>  8
cgaggtctcc tattaatgct tgtcaccctc g                                      31


<210>  9
<211>  73
<212>  DNA
<213>  artificial

<220>
<223>  3' part of the 3' UTR

<400>  9
aaaaaaaatg catccccccc cccccccccc cccccccccc ccccaaaggc tcttttcaga       60

gccaccagaa ttc                                                          73


<210>  10
<211>  64
<212>  DNA
<213>  artificial

<220>
<223>  primer CU forward

<400>  10
gtcctcatgc atcccccccc cccccccccc cccccccccc cccacaagca acttctcttc       60

aaca                                                                    64


<210>  11
<211>  56
<212>  DNA
<213>  artificial

<220>
<223>  primer CU reverse

<400>  11
catggtgaat tctggtggct ctgaaaagag ccttttgttg aagagaagtt gcttgt           56


<210>  12
<211>  64
<212>  DNA
<213>  artificial

<220>
<223>  primer FR forward

<400>  12
gtcctcatgc atcccccccc cccccccccc cccccccccc cccagaaata cagttagctc       60

ggaa                                                                    64


<210>  13
<211>  56
<212>  DNA
<213>  artificial

<220>
<223>  primer FR reverse

<400>  13
catggtgaat tctggtggct ctgaaaagag cctttttccg agctaactgt atttct           56


<210>  14
<211>  64
<212>  DNA
<213>  artificial

<220>
<223>  primer NU forward

<400>  14
gtcctcatgc atcccccccc cccccccccc cccccccccc cccgttaatt tcaagacatc       60

agtt                                                                    64


<210>  15
<211>  56
<212>  DNA
<213>  artificial

<220>
<223>  primer NU reverse

<400>  15
catggtgaat tctggtggct ctgaaaagag cctttaactg atgtcttgaa attaac           56


<210>  16
<211>  64
<212>  DNA
<213>  artificial

<220>
<223>  primer SP forward

<400>  16
gtcctcatgc atcccccccc cccccccccc cccccccccc cccgtgacaa gcattaatag       60

gaga                                                                    64


<210>  17
<211>  56
<212>  DNA
<213>  artificial

<220>
<223>  primer SP reverse

<400>  17
catggtgaat tctggtggct ctgaaaagag ccttttctcc tattaatgct tgtcac           56


<210>  18
<211>  21
<212>  RNA
<213>  artificial

<220>
<223>  CU target oligonucleotide

<400>  18
acaagcaacu ucucuucaac a                                                 21


<210>  19
<211>  21
<212>  RNA
<213>  artificial

<220>
<223>  FR target oligonucleotide

<400>  19
agaaauacag uuagcucgga a                                                 21


<210>  20
<211>  21
<212>  RNA
<213>  artificial

<220>
<223>  NU target oligonucleotide

<400>  20
guuaauuuca agacaucagu u                                                 21


<210>  21
<211>  21
<212>  RNA
<213>  artificial

<220>
<223>  SP target oligonucleotide

<400>  21
gugacaagca uuaauaggag a                                                 21


<210>  22
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  DNA probe S1

<400>  22
gtcccacttc ttgttctgga agccgtc                                           27


<210>  23
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  DNA probe S4

<400>  23
ccaggagctc gcggtggaca ggctctc                                           27


<210>  24
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  DNA probe S8

<400>  24
atggtcgcga agaagccgct ct                                                22


<210>  25
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  DNA probe S10

<400>  25
cacgcgggcg gacgggatct tgccggt                                           27


<210>  26
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  probe S1_CU

<400>  26
tgttgaagag aagttgcttg t                                                 21


<210>  27
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  probe S1_FR

<400>  27
ttccgagcta actgtatttc t                                                 21


<210>  28
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  probe S1_NU

<400>  28
aactgatgtc ttgaaattaa c                                                 21


<210>  29
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  probe S1_SP

<400>  29
tctcctatta atgcttgtca c                                                 21


<210>  30
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  H3N2 forward primer

<400>  30
cccatcggca agtgcaagtc                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  H3N2 reverse primer

<400>  31
ccggttcacg ttctggaagg                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  H1N1 forward primer

<400>  32
ggagaagcgg atcgagaacc                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  H1N1 reverse primer

<400>  33
cggcgttgta cgtccagatg                                                   20


<210>  34
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  influenza B massachusetts forward primer

<400>  34
ccaaccccct gacggtg                                                      17


<210>  35
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  influenza B Massachusetts reverse primer

<400>  35
tgtcgccgta caggttcttc                                                   20


<210>  36
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  influenza B Brisbane forward primer

<400>  36
cacccacaac gtgatcaacg c                                                 21


<210>  37
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  influenza B Brisbane reverse primer

<400>  37
gttgccgttc gtgatgttgg                                                   20


