                               SEQUENCE LISTING

<110> UNIVERSITY OF MASSACHUSETTS
 
<120> BRANCHED OLIGONUCLEOTIDES

<130> 586594: UM9-213PC

<140> PCT/US2017/015633
<141> 2017-01-30

<150> 62/317,113
<151> 2016-04-01

<150> 62/289,268
<151> 2016-01-31

<160> 5     

<170> PatentIn version 3.5

<210> 1
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1) 
<223> Backbone-modified-U

<220>
<221> misc_feature
<222> (1)..(3)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (2)..(2) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (3)..(3) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (4)..(4) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (5)..(5) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (6)..(6) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (7)..(7) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (8)..(8) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (9)..(9) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (10)..(10) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (11)..(11) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (12)..(12) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (13)..(13) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (14)..(14) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> misc_feature
<222> (14)..(20)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (15)..(15) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (16)..(16) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (17)..(17) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (18)..(18) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (19)..(19) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (20)..(20) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220> 
<223> See specification as filed for detailed description of
      substitutions and preferred embodiments

<400> 1
uuaaucucuu uacugauaua                                                   20


<210> 2
<211> 15
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> misc_feature
<222> (1)..(3)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (2)..(2) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (3)..(3) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (4)..(4) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (5)..(5) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (6)..(6) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (7)..(7) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (8)..(8) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (9)..(9) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (10)..(10) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (11)..(11) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (12)..(12) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (13)..(13) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> misc_feature
<222> (13)..(15)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (14)..(14) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (15)..(15) 
<223> 2'-deoxy-2'-fluoro nucleotide

<400> 2
caguaaagag auuaa                                                        15


<210> 3
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1) 
<223> Backbone-modified-U

<220>
<221> misc_feature
<222> (1)..(3)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (2)..(2) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (3)..(3) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (4)..(4) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (5)..(5) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (6)..(6) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (7)..(7) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (8)..(8) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (9)..(9) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (10)..(10) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (11)..(11) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (12)..(12) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (13)..(13) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (14)..(14) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> misc_feature
<222> (14)..(20)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (15)..(15) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (16)..(16) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (17)..(17) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (18)..(18) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (19)..(19) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (20)..(20) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220> 
<223> See specification as filed for detailed description of
      substitutions and preferred embodiments

<400> 3
uuaaucucuu uacugauaua                                                   20


<210> 4
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> misc_feature
<222> (1)..(3)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (2)..(2) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (3)..(3) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (4)..(4) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (5)..(5) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (6)..(6) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (7)..(7) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (8)..(8) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (9)..(9) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (10)..(10) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (11)..(11) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (12)..(12) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (13)..(13) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> misc_feature
<222> (13)..(20)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (14)..(14) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (15)..(15) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (16)..(16) 
<223> 2'-O-methyl nucleotide; a, c, g or u

<220>
<221> modified_base
<222> (17)..(17) 
<223> 2'-deoxy-2'-fluoro nucleotide; a, c, g or u

<220>
<221> modified_base
<222> (18)..(18) 
<223> 2'-O-methyl nucleotide; a, c, g or u

<220>
<221> modified_base
<222> (19)..(19) 
<223> 2'-deoxy-2'-fluoro nucleotide; a, c, g or u

<220>
<221> modified_base
<222> (20)..(20) 
<223> 2'-O-methyl nucleotide; a, c, g or u

<400> 4
caguaaagag auuaannnnn                                                   20


<210> 5
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1) 
<223> 5'-E-VP-mU

<220>
<221> misc_feature
<222> (1)..(3)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (2)..(2) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (3)..(3) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (4)..(4) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (5)..(5) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (6)..(6) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (7)..(7) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (8)..(8) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (9)..(9) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (10)..(10) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (11)..(11) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (12)..(12) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (13)..(13) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (14)..(14) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (14)..(14) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (15)..(15) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (16)..(16) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (17)..(17) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (18)..(18) 
<223> 2'-deoxy-2'-fluoro nucleotide

<220>
<221> modified_base
<222> (19)..(19) 
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (20)..(20) 
<223> 2'-deoxy-2'-fluoro nucleotide

<400> 5
uuaaucucuu uacugauaua                                                   20



