﻿               SEQUENCE LISTING

<110> Sirion Biotech GmbH
      Ludwig-Maximilians-Universität München

<120> AAV-based conditional expression system

<130> X3009 PCT

<150> EP 16 15 3329.4
<151> 2016-01-29

<160> 10

<170> BiSSAP 1.3

<210> 1
<211> 8977
<212> DNA
<213> Artificial Sequence

<220> 
<223> Conditional expression system

<400> 1
atcgataagc ttctagagat ctgggccact ccctctctgc gcgctcgctc gctcactgag       60

gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag      120

cgagcgcgca gagagggagt ggccaactcc atcactaggg gttcctggag gggtggagtc      180

gtgacgtgaa ttacgtcata gggttaggga ggtcctgtat tagaggtcac gtgagtgttt      240

tgcgacattt tgcgacacca tgtggtcacg ctgggtattt aagcccgagt gagcacgcag      300

ggtctccatt ttgaagcggg aggtttgaac gcgcagccgc catgccgggg ttttacgaga      360

ttgtgattaa ggtccccagc gaccttgacg ggcatctgcc cggcatttct gacagctttg      420

tgaactgggt ggccgagaag gaatgggagt tgccgccaga ttctgacatg gatctgaatc      480

tgattgagca ggcacccctg accgtggccg agaagctgca gcgcgacttt ctgacggaat      540

ggcgccgtgt gagtaaggcc ccggaggccc ttttctttgt gcaatttgag aagggagaga      600

gctacttcca catgcacgtg ctcgtggaaa ccaccggggt gaaatccatg gttttgggac      660

gtttcctgag tcagattcgc gaaaaactga ttcagagaat ttaccgcggg atcgagccga      720

ctttgccaaa ctggttcgcg gtcacaaaga ccagaaatgg cgccggaggc gggaacaagg      780

tggtggatga gtgctacatc cccaattact tgctccccaa aacccagcct gagctccagt      840

gggcgtggac taatatggaa cagtatttaa gcgcctgttt gaatctcacg gagcgtaaac      900

ggttggtggc gcagcatctg acgcacgtgt cgcagacgca ggagcagaac aaagagaatc      960

agaatcccaa ttctgatgcg ccggtgatca gatcaaaaac ttcagccagg tacatggagc     1020

tggtcgggtg gctcgtggac aaggggatta cctcggagaa gcagtggatc caggaggacc     1080

aggcctcata catctccttc aatgcggcct ccaactcgcg gtcccaaatc aaggctgcct     1140

tggacaatgc gggaaagatt atgagcctga ctaaaaccgc ccccgactac ctggtgggcc     1200

agcagcccgt ggaggacatt tccagcaatc ggatttataa aattttggaa ctaaacgggt     1260

acgatcccca atatgcggct tccgtctttc tgggatgggc cacgaaaaag ttcggcaaga     1320

ggaacaccat ctggctgttt gggcctgcaa ctaccgggaa gaccaacatc gcggaggcca     1380

tagcccacac tgtgcccttc tacgggtgcg taaactggac caatgagaac tttcccttca     1440

acgactgtgt cgacaagatg gtgatctggt gggaggaggg gaagatgacc gccaaggtcg     1500

tggagtcggc caaagccatt ctcggaggaa gcaaggtgcg cgtggaccag aaatgcaagt     1560

cctcggccca gatagacccg actcccgtga tcgtcacctc caacaccaac atgtgcgccg     1620

tgattgacgg gaactcaacg accttcgaac accagcagcc gttgcaagac cggatgttca     1680

aatttgaact cacccgccgt ctggatcatg actttgggaa ggtcaccaag caggaagtca     1740

aagacttttt ccggtgggca aaggatcacg tggttgaggt ggagcatgaa ttctacgtca     1800

aaaagggtgg agccaagaaa agacccgccc ccagtgacgc agatataagt gagcccaaac     1860

gggtgcgcga gtcagttgcg cagccatcga cgtcagacgc ggaagcttcg atcaactacg     1920

cagacaggta ccaaaacaaa tgttctcgtc acgtgggcat gaatctgatg ctgtttccct     1980

gcagacaatg cgagagaatg aatcagaatt caaatatctg cttcactcac ggacagaaag     2040

actgtttaga gtgctttccc gtgtcagaat ctcaacccgt ttctgtcgtc aaaaaggcgt     2100

atcagaaact gtgctacatt catcatatca tgggaaaggt gccagacgct tgcactgcct     2160

gcgatctggt caatgtggat ttggatgact gcatctttga acaataaatg atttaaatca     2220

ggtatggctg ccgatggtta tcttccagat tggctcgagg acactctctc tgagctagct     2280

tcgtacggat cctcggatcc agccaccatg ggagtcaaag ttctgtttgc cctgatctgc     2340

atcgctgtgg ccgaggccaa gcccaccgag aacaacgaag acttcaacat cgtggccgtg     2400

gccagcaact tcgcgaccac ggatctcgat gctgaccgcg ggaagttgcc cggcaagaag     2460

ctgccgctgg aggtgctcaa agagatggaa gccaatgccc ggaaagctgg ctgcaccagg     2520

ggctgtctga tctgcctgtc ccacatcaag tgcacgccca agatgaagaa gttcatccca     2580

ggacgctgcc acacctacga aggcgacaaa gagtccgcac agggcggcat aggcgaggcg     2640

atcgtcgaca ttcctgagat tcctgggttc aaggacttgg agcccatgga gcagttcatc     2700

gcacaggtcg atctgtgtgt ggactgcaca actggctgcc tcaaagggct tgccaacgtg     2760

cagtgttctg acctgctcaa gaagtggctg ccgcaacgct gtgcgacctt tgccagcaag     2820

atccagggcc aggtggacaa gatcaagggg gccggtggtg actaagcggc cgcgtgtgga     2880

aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca     2940

accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc     3000

aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc     3060

agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag     3120

gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc     3180

ttttgcaaaa agcttccatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc     3240

tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc     3300

gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg     3360

atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc     3420

cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg     3480

cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg     3540

tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc     3600

cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg     3660

ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg     3720

cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg     3780

tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca     3840

ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct     3900

ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg     3960

agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct     4020

atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg     4080

caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg     4140

ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca     4200

ctcgtccgag ggcaaaggaa tagagttcta gaggatcata atcagccata ccacagggcc     4260

catctgggca aagattccac acacggacgg acattttcac ccctctcccc tcatgggtgg     4320

attcggactt aaacaccctc ctccacagat tctcatcaag aacaccccgg tacctgcgaa     4380

tccttcgacc accttcagtg cggcaaagtt tgcttccttc atcacacagt actccacggg     4440

acacggtcag cgtggagatc gagtgggagc tgcagaagga aaacagcaaa cgctggaatc     4500

ccgaaattca gtacacttcc aactacaaca agtctgttaa tcgtggactt accgtggata     4560

ctaatggcgt gtattcagag cctcgcccca ttggcaccag atacctgact cgtaatctgt     4620

aattgcttgt taatcaataa accgtttaat tcgtttcagt tgaactttgg tctctgcgta     4680

tttctttctt atctagtttc catggctacg tagataagta gcatggcggg ttaatcatta     4740

actacaagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca     4800

ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga     4860

gcgagcgagc gcgcagagag ggacagatct tccataccta ccagttctgc gcctgcagca     4920

atggcaacaa cgttgcccgg atccggtcgc gcgaattctt gaagacgaaa gggcctcgtg     4980

atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc     5040

acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat     5100

atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag     5160

agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt     5220

cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt     5280

gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc     5340

cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta     5400

tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac     5460

ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa     5520

ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg     5580

atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc     5640

cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg     5700

atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta     5760

gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg     5820

cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg     5880

tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc     5940

tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt     6000

gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt     6060

gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc     6120

atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag     6180

atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa     6240

aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg     6300

aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag     6360

ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg     6420

ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga     6480

tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc     6540

ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc     6600

acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga     6660

gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt     6720

cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg     6780

aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac     6840

atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga     6900

gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg     6960

gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata     7020

tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc     7080

tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc     7140

cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga     7200

gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa     7260

gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct     7320

cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg     7380

cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg     7440

taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg     7500

cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga     7560

gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg     7620

gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc     7680

gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg     7740

cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct     7800

aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc     7860

gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg     7920

cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga     7980

attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat     8040

tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta     8100

tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat     8160

cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc     8220

ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg     8280

catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt     8340

cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg     8400

cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa     8460

gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc     8520

gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac     8580

agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt     8640

gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca     8700

gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga     8760

gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc     8820

gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc     8880

gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagagctc     8940

tagagctcta gagaattctc atgtttgaca gcttatc                              8977


<210> 2
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 2
gtgtaggcct cggatccagc caccatggga gtc                                  33


<210> 3
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 3
ccatagagcc caccgcatcc cc                                             22


<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 4
tggtatcgtg gaaggactca                                                20


<210> 5
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 5
ccagtagagg cagggatgat                                                20


<210> 6
<211> 536
<212> PRT
<213> Adeno-associated virus - 2

<220> 
<223> Rep 68

<400> 6
Met Pro Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile 
        35                  40                  45              
Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val 
65                  70                  75                  80  
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      
Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         
Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     
Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala 
                485                 490                 495     
Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val 
            500                 505                 510         
Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp 
        515                 520                 525             
Arg Leu Ala Arg Gly His Ser Leu 
    530                 535     

<210> 7
<211> 621
<212> PRT
<213> Adeno-associated virus - 2

<220> 
<223> Rep 78

<400> 7
Met Pro Gly Phe Tyr Glu Ile Val Ile Lys Val Pro Ser Asp Leu Asp 
1               5                   10                  15      
Glu His Leu Pro Gly Ile Ser Asp Ser Phe Val Asn Trp Val Ala Glu 
            20                  25                  30          
Lys Glu Trp Glu Leu Pro Pro Asp Ser Asp Met Asp Leu Asn Leu Ile 
        35                  40                  45              
Glu Gln Ala Pro Leu Thr Val Ala Glu Lys Leu Gln Arg Asp Phe Leu 
    50                  55                  60                  
Thr Glu Trp Arg Arg Val Ser Lys Ala Pro Glu Ala Leu Phe Phe Val 
65                  70                  75                  80  
Gln Phe Glu Lys Gly Glu Ser Tyr Phe His Met His Val Leu Val Glu 
                85                  90                  95      
Thr Thr Gly Val Lys Ser Met Val Leu Gly Arg Phe Leu Ser Gln Ile 
            100                 105                 110         
Arg Glu Lys Leu Ile Gln Arg Ile Tyr Arg Gly Ile Glu Pro Thr Leu 
        115                 120                 125             
Pro Asn Trp Phe Ala Val Thr Lys Thr Arg Asn Gly Ala Gly Gly Gly 
    130                 135                 140                 
Asn Lys Val Val Asp Glu Cys Tyr Ile Pro Asn Tyr Leu Leu Pro Lys 
145                 150                 155                 160 
Thr Gln Pro Glu Leu Gln Trp Ala Trp Thr Asn Met Glu Gln Tyr Leu 
                165                 170                 175     
Ser Ala Cys Leu Asn Leu Thr Glu Arg Lys Arg Leu Val Ala Gln His 
            180                 185                 190         
Leu Thr His Val Ser Gln Thr Gln Glu Gln Asn Lys Glu Asn Gln Asn 
        195                 200                 205             
Pro Asn Ser Asp Ala Pro Val Ile Arg Ser Lys Thr Ser Ala Arg Tyr 
    210                 215                 220                 
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
225                 230                 235                 240 
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
                245                 250                 255     
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
            260                 265                 270         
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
        275                 280                 285             
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
    290                 295                 300                 
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
305                 310                 315                 320 
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
                325                 330                 335     
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
            340                 345                 350         
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
        355                 360                 365             
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
    370                 375                 380                 
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
385                 390                 395                 400 
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
                405                 410                 415     
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
            420                 425                 430         
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
        435                 440                 445             
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
    450                 455                 460                 
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
465                 470                 475                 480 
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala 
                485                 490                 495     
Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val 
            500                 505                 510         
Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp 
        515                 520                 525             
Arg Tyr Gln Asn Lys Cys Ser Arg His Val Gly Met Asn Leu Met Leu 
    530                 535                 540                 
Phe Pro Cys Arg Gln Cys Glu Arg Met Asn Gln Asn Ser Asn Ile Cys 
545                 550                 555                 560 
Phe Thr His Gly Gln Lys Asp Cys Leu Glu Cys Phe Pro Val Ser Glu 
                565                 570                 575     
Ser Gln Pro Val Ser Val Val Lys Lys Ala Tyr Gln Lys Leu Cys Tyr 
            580                 585                 590         
Ile His His Ile Met Gly Lys Val Pro Asp Ala Cys Thr Ala Cys Asp 
        595                 600                 605             
Leu Val Asn Val Asp Leu Asp Asp Cys Ile Phe Glu Gln 
    610                 615                 620     

<210> 8
<211> 312
<212> PRT
<213> Adeno-associated virus - 2

<220> 
<223> Rep 40

<400> 8
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
1               5                   10                  15      
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
            20                  25                  30          
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
        35                  40                  45              
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
    50                  55                  60                  
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
65                  70                  75                  80  
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
                85                  90                  95      
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
            100                 105                 110         
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
        115                 120                 125             
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
    130                 135                 140                 
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
145                 150                 155                 160 
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
                165                 170                 175     
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
            180                 185                 190         
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
        195                 200                 205             
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
    210                 215                 220                 
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
225                 230                 235                 240 
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
                245                 250                 255     
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala 
            260                 265                 270         
Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val 
        275                 280                 285             
Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp 
    290                 295                 300                 
Arg Leu Ala Arg Gly His Ser Leu 
305                 310         

<210> 9
<211> 397
<212> PRT
<213> Adeno-associated virus - 2

<220> 
<223> Rep 52

<400> 9
Met Glu Leu Val Gly Trp Leu Val Asp Lys Gly Ile Thr Ser Glu Lys 
1               5                   10                  15      
Gln Trp Ile Gln Glu Asp Gln Ala Ser Tyr Ile Ser Phe Asn Ala Ala 
            20                  25                  30          
Ser Asn Ser Arg Ser Gln Ile Lys Ala Ala Leu Asp Asn Ala Gly Lys 
        35                  40                  45              
Ile Met Ser Leu Thr Lys Thr Ala Pro Asp Tyr Leu Val Gly Gln Gln 
    50                  55                  60                  
Pro Val Glu Asp Ile Ser Ser Asn Arg Ile Tyr Lys Ile Leu Glu Leu 
65                  70                  75                  80  
Asn Gly Tyr Asp Pro Gln Tyr Ala Ala Ser Val Phe Leu Gly Trp Ala 
                85                  90                  95      
Thr Lys Lys Phe Gly Lys Arg Asn Thr Ile Trp Leu Phe Gly Pro Ala 
            100                 105                 110         
Thr Thr Gly Lys Thr Asn Ile Ala Glu Ala Ile Ala His Thr Val Pro 
        115                 120                 125             
Phe Tyr Gly Cys Val Asn Trp Thr Asn Glu Asn Phe Pro Phe Asn Asp 
    130                 135                 140                 
Cys Val Asp Lys Met Val Ile Trp Trp Glu Glu Gly Lys Met Thr Ala 
145                 150                 155                 160 
Lys Val Val Glu Ser Ala Lys Ala Ile Leu Gly Gly Ser Lys Val Arg 
                165                 170                 175     
Val Asp Gln Lys Cys Lys Ser Ser Ala Gln Ile Asp Pro Thr Pro Val 
            180                 185                 190         
Ile Val Thr Ser Asn Thr Asn Met Cys Ala Val Ile Asp Gly Asn Ser 
        195                 200                 205             
Thr Thr Phe Glu His Gln Gln Pro Leu Gln Asp Arg Met Phe Lys Phe 
    210                 215                 220                 
Glu Leu Thr Arg Arg Leu Asp His Asp Phe Gly Lys Val Thr Lys Gln 
225                 230                 235                 240 
Glu Val Lys Asp Phe Phe Arg Trp Ala Lys Asp His Val Val Glu Val 
                245                 250                 255     
Glu His Glu Phe Tyr Val Lys Lys Gly Gly Ala Lys Lys Arg Pro Ala 
            260                 265                 270         
Pro Ser Asp Ala Asp Ile Ser Glu Pro Lys Arg Val Arg Glu Ser Val 
        275                 280                 285             
Ala Gln Pro Ser Thr Ser Asp Ala Glu Ala Ser Ile Asn Tyr Ala Asp 
    290                 295                 300                 
Arg Tyr Gln Asn Lys Cys Ser Arg His Val Gly Met Asn Leu Met Leu 
305                 310                 315                 320 
Phe Pro Cys Arg Gln Cys Glu Arg Met Asn Gln Asn Ser Asn Ile Cys 
                325                 330                 335     
Phe Thr His Gly Gln Lys Asp Cys Leu Glu Cys Phe Pro Val Ser Glu 
            340                 345                 350         
Ser Gln Pro Val Ser Val Val Lys Lys Ala Tyr Gln Lys Leu Cys Tyr 
        355                 360                 365             
Ile His His Ile Met Gly Lys Val Pro Asp Ala Cys Thr Ala Cys Asp 
    370                 375                 380                 
Leu Val Asn Val Asp Leu Asp Asp Cys Ile Phe Glu Gln 
385                 390                 395         

<210> 10
<211> 4854
<212> DNA
<213> Artificial Sequence

<220> 
<223> Conditional expression system

<400> 10
tgggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc       60

cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg      120

gccaactcca tcactagggg ttcctggagg ggtggagtcg tgacgtgaat tacgtcatag      180

ggttagggag gtcctgtatt agaggtcacg tgagtgtttt gcgacatttt gcgacaccat      240

gtggtcacgc tgggtattta agcccgagtg agcacgcagg gtctccattt tgaagcggga      300

ggtttgaacg cgcagccgcc atgccggggt tttacgagat tgtgattaag gtccccagcg      360

accttgacgg gcatctgccc ggcatttctg acagctttgt gaactgggtg gccgagaagg      420

aatgggagtt gccgccagat tctgacatgg atctgaatct gattgagcag gcacccctga      480

ccgtggccga gaagctgcag cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc      540

cggaggccct tttctttgtg caatttgaga agggagagag ctacttccac atgcacgtgc      600

tcgtggaaac caccggggtg aaatccatgg ttttgggacg tttcctgagt cagattcgcg      660

aaaaactgat tcagagaatt taccgcggga tcgagccgac tttgccaaac tggttcgcgg      720

tcacaaagac cagaaatggc gccggaggcg ggaacaaggt ggtggatgag tgctacatcc      780

ccaattactt gctccccaaa acccagcctg agctccagtg ggcgtggact aatatggaac      840

agtatttaag cgcctgtttg aatctcacgg agcgtaaacg gttggtggcg cagcatctga      900

cgcacgtgtc gcagacgcag gagcagaaca aagagaatca gaatcccaat tctgatgcgc      960

cggtgatcag atcaaaaact tcagccaggt acatggagct ggtcgggtgg ctcgtggaca     1020

aggggattac ctcggagaag cagtggatcc aggaggacca ggcctcatac atctccttca     1080

atgcggcctc caactcgcgg tcccaaatca aggctgcctt ggacaatgcg ggaaagatta     1140

tgagcctgac taaaaccgcc cccgactacc tggtgggcca gcagcccgtg gaggacattt     1200

ccagcaatcg gatttataaa attttggaac taaacgggta cgatccccaa tatgcggctt     1260

ccgtctttct gggatgggcc acgaaaaagt tcggcaagag gaacaccatc tggctgtttg     1320

ggcctgcaac taccgggaag accaacatcg cggaggccat agcccacact gtgcccttct     1380

acgggtgcgt aaactggacc aatgagaact ttcccttcaa cgactgtgtc gacaagatgg     1440

tgatctggtg ggaggagggg aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc     1500

tcggaggaag caaggtgcgc gtggaccaga aatgcaagtc ctcggcccag atagacccga     1560

ctcccgtgat cgtcacctcc aacaccaaca tgtgcgccgt gattgacggg aactcaacga     1620

ccttcgaaca ccagcagccg ttgcaagacc ggatgttcaa atttgaactc acccgccgtc     1680

tggatcatga ctttgggaag gtcaccaagc aggaagtcaa agactttttc cggtgggcaa     1740

aggatcacgt ggttgaggtg gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa     1800

gacccgcccc cagtgacgca gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc     1860

agccatcgac gtcagacgcg gaagcttcga tcaactacgc agacaggtac caaaacaaat     1920

gttctcgtca cgtgggcatg aatctgatgc tgtttccctg cagacaatgc gagagaatga     1980

atcagaattc aaatatctgc ttcactcacg gacagaaaga ctgtttagag tgctttcccg     2040

tgtcagaatc tcaacccgtt tctgtcgtca aaaaggcgta tcagaaactg tgctacattc     2100

atcatatcat gggaaaggtg ccagacgctt gcactgcctg cgatctggtc aatgtggatt     2160

tggatgactg catctttgaa caataaatga tttaaatcag gtatggctgc cgatggttat     2220

cttccagatt ggctcgagga cactctctct gagctagctt cgtacggatc ctcggatcca     2280

gccaccatgg gagtcaaagt tctgtttgcc ctgatctgca tcgctgtggc cgaggccaag     2340

cccaccgaga acaacgaaga cttcaacatc gtggccgtgg ccagcaactt cgcgaccacg     2400

gatctcgatg ctgaccgcgg gaagttgccc ggcaagaagc tgccgctgga ggtgctcaaa     2460

gagatggaag ccaatgcccg gaaagctggc tgcaccaggg gctgtctgat ctgcctgtcc     2520

cacatcaagt gcacgcccaa gatgaagaag ttcatcccag gacgctgcca cacctacgaa     2580

ggcgacaaag agtccgcaca gggcggcata ggcgaggcga tcgtcgacat tcctgagatt     2640

cctgggttca aggacttgga gcccatggag cagttcatcg cacaggtcga tctgtgtgtg     2700

gactgcacaa ctggctgcct caaagggctt gccaacgtgc agtgttctga cctgctcaag     2760

aagtggctgc cgcaacgctg tgcgaccttt gccagcaaga tccagggcca ggtggacaag     2820

atcaaggggg ccggtggtga ctaagcggcc gcgtgtggaa agtccccagg ctccccagca     2880

ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca     2940

ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc     3000

ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc     3060

catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta     3120

ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttccatga     3180

aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg     3240

tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag     3300

gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt     3360

atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg     3420

aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag     3480

acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga     3540

tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg     3600

gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact     3660

ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga     3720

tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca     3780

acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt     3840

tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta     3900

tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc     3960

tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca     4020

atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg     4080

ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg     4140

tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat     4200

agagttctag aggatcataa tcagccatac cacagggccc atctgggcaa agattccaca     4260

cacggacgga cattttcacc cctctcccct catgggtgga ttcggactta aacaccctcc     4320

tccacagatt ctcatcaaga acaccccggt acctgcgaat ccttcgacca ccttcagtgc     4380

ggcaaagttt gcttccttca tcacacagta ctccacggga cacggtcagc gtggagatcg     4440

agtgggagct gcagaaggaa aacagcaaac gctggaatcc cgaaattcag tacacttcca     4500

actacaacaa gtctgttaat cgtggactta ccgtggatac taatggcgtg tattcagagc     4560

ctcgccccat tggcaccaga tacctgactc gtaatctgta attgcttgtt aatcaataaa     4620

ccgtttaatt cgtttcagtt gaactttggt ctctgcgtat ttctttctta tctagtttcc     4680

atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga     4740

tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg     4800

tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgca           4854


