                         SEQUENCE LISTING

<110>  De Staat der Nederlanden, vert. door de minister van VWS, 
       Ministerie van Volksgezondheid, Welzijn en Sport
 
<120>  Modified tetra-acylated neisserial LPS

<130>  P6059084PCT

<150>  NL2016169
<151>  2016-01-28

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  299
<212>  PRT
<213>  Neisseria meningitidis

<400>  1

Met Pro Ser Glu Lys Lys Met Cys Ile Glu Met Lys Phe Ile Phe Phe 
1               5                   10                  15      


Val Leu Tyr Val Leu Gln Phe Leu Pro Phe Ala Leu Leu His Lys Ile 
            20                  25                  30          


Ala Asp Leu Thr Gly Leu Leu Ala Tyr Leu Leu Val Lys Pro Arg Arg 
        35                  40                  45              


Arg Ile Gly Glu Ile Asn Leu Ala Lys Cys Phe Ser Glu Trp Ser Glu 
    50                  55                  60                  


Glu Lys Arg Lys Thr Val Leu Lys Gln His Phe Lys His Met Ala Lys 
65                  70                  75                  80  


Leu Met Leu Glu Tyr Gly Leu Tyr Trp Tyr Ala Pro Ala Gly Arg Leu 
                85                  90                  95      


Lys Ser Leu Val Arg Tyr Arg Asn Lys His Tyr Leu Asp Asp Ala Leu 
            100                 105                 110         


Ala Ala Gly Glu Lys Val Ile Ile Leu Tyr Pro His Phe Thr Ala Phe 
        115                 120                 125             


Glu Met Ala Val Tyr Ala Leu Asn Gln Asp Ile Pro Leu Ile Ser Met 
    130                 135                 140                 


Tyr Ser His Gln Lys Asn Lys Ile Leu Asp Glu Gln Ile Leu Lys Gly 
145                 150                 155                 160 


Arg Asn Arg Tyr His Asn Val Phe Leu Ile Gly Arg Thr Glu Gly Leu 
                165                 170                 175     


Arg Ala Leu Val Lys Gln Phe Arg Lys Ser Ser Ala Pro Phe Leu Tyr 
            180                 185                 190         


Leu Pro Asp Gln Asp Phe Gly Arg Asn Asp Ser Val Phe Val Asp Phe 
        195                 200                 205             


Phe Gly Ile Gln Thr Ala Thr Ile Thr Gly Leu Ser Arg Ile Ala Ala 
    210                 215                 220                 


Leu Ala Asn Ala Lys Val Ile Pro Ala Ile Pro Val Arg Glu Ala Asp 
225                 230                 235                 240 


Asn Thr Val Thr Leu His Phe Tyr Pro Ala Trp Lys Ser Phe Pro Gly 
                245                 250                 255     


Glu Asp Ala Lys Ala Asp Ala Gln Arg Met Asn Arg Phe Ile Glu Asp 
            260                 265                 270         


Arg Val Arg Glu His Pro Glu Gln Tyr Phe Trp Leu His Lys Arg Phe 
        275                 280                 285             


Lys Thr Arg Pro Glu Gly Ser Pro Asp Phe Tyr 
    290                 295                 


<210>  2
<211>  293
<212>  PRT
<213>  Neisseria gonorrhoeae

<400>  2

Met Cys Thr Glu Met Lys Phe Ile Phe Phe Val Leu Tyr Val Leu Gln 
1               5                   10                  15      


Phe Leu Pro Phe Ala Leu Leu His Lys Ile Ala Gly Leu Ile Gly Ser 
            20                  25                  30          


Leu Ala Tyr Leu Leu Val Lys Pro Arg Arg Arg Ile Gly Glu Ile Asn 
        35                  40                  45              


Leu Ala Lys Cys Phe Pro Glu Trp Asp Glu Glu Lys Arg Lys Thr Val 
    50                  55                  60                  


Leu Lys Gln His Phe Lys His Met Ala Lys Leu Met Leu Glu Tyr Gly 
65                  70                  75                  80  


Leu Tyr Trp Tyr Ala Pro Ala Lys Cys Leu Lys Ser Met Val Arg Tyr 
                85                  90                  95      


Arg Asn Lys His Tyr Leu Asp Asp Ala Leu Ala Ala Gly Glu Lys Val 
            100                 105                 110         


Ile Ile Leu Tyr Pro His Phe Thr Ala Phe Glu Met Ala Val Tyr Ala 
        115                 120                 125             


Leu Asn Gln Asp Val Pro Leu Ile Ser Met Tyr Ser His Gln Lys Asn 
    130                 135                 140                 


Lys Ile Leu Asp Glu Gln Ile Leu Lys Gly Arg Asn Arg Tyr His Asn 
145                 150                 155                 160 


Val Phe Leu Ile Gly Arg Thr Glu Gly Leu Arg Ala Leu Val Lys Gln 
                165                 170                 175     


Phe Arg Lys Ser Ser Ala Pro Phe Leu Tyr Leu Pro Asp Gln Asp Phe 
            180                 185                 190         


Gly Arg Asn Asn Ser Val Phe Val Asp Phe Phe Gly Ile Gln Thr Ala 
        195                 200                 205             


Thr Ile Thr Gly Leu Ser Arg Ile Ala Ala Leu Ala Asn Ala Lys Val 
    210                 215                 220                 


Ile Pro Ala Ile Pro Val Arg Glu Ala Asp Asn Thr Val Thr Leu Gln 
225                 230                 235                 240 


Phe Tyr Pro Ala Trp Lys Ser Phe Pro Ser Glu Asp Ala Gln Ala Asp 
                245                 250                 255     


Ala Gln Arg Met Asn Arg Phe Ile Glu Glu Arg Val Arg Glu His Pro 
            260                 265                 270         


Glu Gln Tyr Phe Trp Leu His Lys Arg Phe Lys Thr Arg Pro Glu Gly 
        275                 280                 285             


Ser Pro Asp Phe Tyr 
    290             


<210>  3
<211>  296
<212>  PRT
<213>  Neisseria lactamica

<400>  3

Met Lys Phe Ile Phe Phe Val Leu Tyr Val Leu Gln Phe Leu Pro Phe 
1               5                   10                  15      


Ala Leu Leu His Lys Leu Ala Asp Leu Thr Gly Leu Leu Ala Tyr Leu 
            20                  25                  30          


Leu Val Lys Pro Arg Arg Arg Ile Gly Glu Ile Asn Leu Ala Lys Cys 
        35                  40                  45              


Phe Pro Glu Trp Asp Gly Lys Lys Arg Glu Thr Val Leu Lys Gln His 
    50                  55                  60                  


Phe Lys His Met Ala Lys Leu Met Leu Glu Tyr Gly Leu Tyr Trp Tyr 
65                  70                  75                  80  


Ala Pro Ala Gly Arg Leu Lys Ser Leu Val Arg Tyr Arg Asn Lys His 
                85                  90                  95      


Tyr Leu Asp Asp Ala Leu Ala Ala Gly Glu Lys Val Ile Ile Leu Tyr 
            100                 105                 110         


Pro His Phe Ile Ala Phe Glu Met Ala Val Tyr Ala Leu Asn Gln Asp 
        115                 120                 125             


Val Pro Leu Ile Ser Met Tyr Ser His Gln Lys Asn Lys Met Leu Asp 
    130                 135                 140                 


Glu Gln Ile Leu Lys Gly Arg Asn Arg Tyr His Asn Val Phe Leu Ile 
145                 150                 155                 160 


Gly Arg Thr Glu Gly Leu Arg Ala Leu Val Lys Gln Phe Arg Lys Ser 
                165                 170                 175     


Ser Ala Pro Phe Leu Tyr Leu Pro Asp Gln Asp Phe Gly Arg Asn Asp 
            180                 185                 190         


Ser Val Phe Val Asp Phe Phe Gly Ile Arg Thr Ala Thr Ile Thr Gly 
        195                 200                 205             


Leu Ser Arg Ile Ala Ala Leu Ala Asn Ala Lys Val Ile Pro Ala Ile 
    210                 215                 220                 


Pro Val Arg Glu Ala Asp Asn Thr Val Thr Leu His Phe Tyr Pro Ala 
225                 230                 235                 240 


Trp Glu Ser Phe Pro Ser Glu Asp Ala Lys Ala Asp Ala Gln Arg Met 
                245                 250                 255     


Asn Arg Phe Ile Glu Glu Arg Val Arg Glu His Pro Glu Gln Tyr Phe 
            260                 265                 270         


Trp Leu His Lys Arg Phe Lys Thr Arg Pro Glu Gly Ser Pro Asp Phe 
        275                 280                 285             


Tyr Gly Leu His Glu Val Thr Lys 
    290                 295     


<210>  4
<211>  283
<212>  PRT
<213>  Neisseria meningitidis

<400>  4

Met His Ile Leu Leu Thr Ala Leu Leu Lys Cys Leu Ser Leu Leu Pro 
1               5                   10                  15      


Leu Ser Cys Leu His Thr Leu Gly Asn Arg Leu Gly His Leu Ala Phe 
            20                  25                  30          


Tyr Leu Leu Lys Glu Asp Arg Ala Arg Ile Val Ala Asn Met Arg Gln 
        35                  40                  45              


Ala Gly Leu Asn Pro Glu Thr Gln Thr Val Lys Ala Val Phe Ala Glu 
    50                  55                  60                  


Thr Ala Lys Gly Gly Leu Glu Leu Ala Pro Ala Phe Phe Lys Lys Pro 
65                  70                  75                  80  


Glu Asp Ile Glu Thr Met Phe Lys Ala Val His Gly Trp Glu His Val 
                85                  90                  95      


Gln Gln Ala Leu Asp Lys Gly Glu Gly Leu Leu Phe Ile Thr Pro His 
            100                 105                 110         


Ile Gly Ser Tyr Asp Leu Gly Gly Arg Tyr Ile Ser Gln Gln Leu Pro 
        115                 120                 125             


Phe Pro Leu Thr Ala Met Tyr Lys Pro Pro Lys Ile Lys Ala Ile Asp 
    130                 135                 140                 


Lys Val Met Gln Ala Gly Arg Val Arg Gly Lys Gly Lys Thr Ala Pro 
145                 150                 155                 160 


Thr Ser Ile Gln Gly Val Lys Gln Ile Ile Lys Ala Leu Arg Ser Gly 
                165                 170                 175     


Glu Ala Thr Ile Val Leu Pro Asp His Val Pro Ser Pro Gln Glu Gly 
            180                 185                 190         


Gly Glu Gly Val Trp Val Asp Phe Phe Gly Lys Pro Ala Tyr Thr Met 
        195                 200                 205             


Thr Leu Ala Ala Lys Leu Ala His Val Lys Gly Val Lys Thr Leu Phe 
    210                 215                 220                 


Phe Cys Cys Glu Arg Leu Pro Asp Gly Gln Gly Phe Ala Leu His Ile 
225                 230                 235                 240 


Ser Pro Phe Gln Gly Lys Leu Thr Gly Asp Lys Thr His Asp Ala Ala 
                245                 250                 255     


Val Phe Asn Arg Asn Thr Glu Tyr Trp Ile Arg Arg Phe Pro Thr Gln 
            260                 265                 270         


Tyr Leu Phe Met Tyr Asn Arg Tyr Lys Met Pro 
        275                 280             


<210>  5
<211>  282
<212>  PRT
<213>  Neisseria gonorrhoeae

<400>  5

Met His Ile Leu Leu Thr Ala Leu Leu Lys Cys Leu Ser Leu Leu Ser 
1               5                   10                  15      


Leu Ser Cys Leu His Thr Leu Gly Asn Arg Leu Gly His Leu Ala Phe 
            20                  25                  30          


Tyr Leu Leu Lys Glu Asp Arg Ala Arg Ile Val Ala Asn Met Arg Gln 
        35                  40                  45              


Ala Gly Leu Asn Pro Asp Thr Gln Thr Val Lys Ala Val Phe Ala Glu 
    50                  55                  60                  


Thr Ala Lys Cys Gly Leu Glu Leu Ala Pro Ala Phe Phe Lys Lys Pro 
65                  70                  75                  80  


Glu Asp Ile Glu Thr Met Phe Lys Ala Val His Gly Trp Glu His Val 
                85                  90                  95      


Gln Gln Ala Leu Asp Lys Gly Glu Gly Leu Leu Phe Ile Thr Pro His 
            100                 105                 110         


Ile Gly Ser Tyr Asp Leu Gly Gly Arg Tyr Ile Ser Gln Gln Leu Pro 
        115                 120                 125             


Phe His Leu Thr Ala Met Tyr Lys Pro Pro Lys Ile Lys Ala Ile Asp 
    130                 135                 140                 


Lys Ile Met Gln Ala Gly Arg Val Arg Gly Lys Gly Lys Thr Ala Pro 
145                 150                 155                 160 


Thr Gly Ile Gln Gly Val Lys Gln Ile Ile Lys Ala Leu Arg Ala Gly 
                165                 170                 175     


Glu Ala Thr Ile Ile Leu Pro Asp His Val Pro Ser Pro Gln Glu Gly 
            180                 185                 190         


Gly Gly Val Trp Ala Asp Phe Phe Gly Lys Pro Ala Tyr Thr Met Thr 
        195                 200                 205             


Leu Ala Ala Lys Leu Ala His Val Lys Gly Val Lys Thr Leu Phe Phe 
    210                 215                 220                 


Cys Cys Glu Arg Leu Pro Asp Gly Gln Gly Phe Val Leu His Ile Arg 
225                 230                 235                 240 


Pro Val Gln Gly Glu Leu Asn Gly Asn Lys Ala His Asp Ala Ala Val 
                245                 250                 255     


Phe Asn Arg Asn Thr Glu Tyr Trp Ile Arg Arg Phe Pro Thr Gln Tyr 
            260                 265                 270         


Leu Phe Met Tyr Asn Arg Tyr Lys Thr Pro 
        275                 280         


<210>  6
<211>  283
<212>  PRT
<213>  Neisseria lactamica

<400>  6

Met His Ile Leu Leu Thr Ala Leu Leu Lys Cys Leu Ser Leu Leu Pro 
1               5                   10                  15      


Leu Pro Ser Leu His Lys Leu Gly Val Phe Leu Gly His Leu Ala Phe 
            20                  25                  30          


Tyr Leu Leu Lys Glu Asp Arg Ala Arg Ile Val Ala Asn Met Arg Gln 
        35                  40                  45              


Ala Gly Leu Asn Pro Asp Thr Gln Thr Val Lys Ala Val Phe Ala Glu 
    50                  55                  60                  


Thr Ala Lys Cys Gly Leu Glu Leu Ala Pro Ala Phe Phe Lys Lys Pro 
65                  70                  75                  80  


Glu Asp Ile Glu Thr Met Phe Lys Ala Val His Gly Trp Glu His Val 
                85                  90                  95      


Gln Gln Ala Leu Ser Lys Gly Glu Gly Leu Leu Phe Ile Thr Pro His 
            100                 105                 110         


Ile Gly Ser Tyr Asp Leu Gly Gly Arg Tyr Ile Ser Gln Arg Leu Pro 
        115                 120                 125             


Phe Pro Leu Thr Ala Met Tyr Lys Pro Pro Lys Ile Lys Ala Ile Asp 
    130                 135                 140                 


Lys Val Met Gln Ala Gly Arg Val Arg Gly Lys Gly Lys Thr Ala Pro 
145                 150                 155                 160 


Ala Gly Ile Gln Gly Val Lys Gln Ile Ile Lys Ala Leu Arg Ala Gly 
                165                 170                 175     


Glu Ala Thr Ile Val Leu Pro Asp Gln Val Pro Ser Pro Gln Glu Gly 
            180                 185                 190         


Gly Asp Gly Val Trp Val Asp Phe Phe Gly Lys Pro Ala Tyr Thr Met 
        195                 200                 205             


Thr Leu Ala Ala Lys Leu Ala Asn Val Lys Gly Val Ser Thr Leu Phe 
    210                 215                 220                 


Phe Cys Ser Glu Arg Leu Pro Asp Gly Gln Gly Phe Val Leu His Ile 
225                 230                 235                 240 


Arg Pro Val Gln Gly Glu Leu Thr Gly Asp Lys Ala His Asp Ala Ala 
                245                 250                 255     


Val Phe Asn Arg Asn Thr Glu Tyr Trp Ile Arg Arg Phe Pro Thr Gln 
            260                 265                 270         


Tyr Leu Phe Met Tyr Asn Arg Tyr Lys Ala Pro 
        275                 280             


<210>  7
<211>  178
<212>  PRT
<213>  Bordetella bronchiseptica

<400>  7

Met Gln Phe Leu Lys Lys Asn Lys Pro Leu Phe Gly Ile Val Thr Leu 
1               5                   10                  15      


Ala Leu Ala Cys Ala Thr Ala Gln Ala Gln Pro Thr Gln Gly Gly Val 
            20                  25                  30          


Ser Leu His Tyr Gly Ile Gly Asp His Tyr Gln Arg Val Thr Leu Asn 
        35                  40                  45              


Tyr Glu Thr Pro Thr Leu Trp Ser His Gln Phe Gly Gly Asn Trp Gly 
    50                  55                  60                  


Arg Leu Asp Leu Thr Pro Glu Leu Gly Ala Ser Tyr Trp Trp Ala Asp 
65                  70                  75                  80  


Gly Ser Arg Ser Pro Gly His Val Trp Gln Ala Ser Ala Ile Pro Met 
                85                  90                  95      


Phe Arg Trp Trp Thr Gly Glu Arg Phe Tyr Ile Glu Ala Gly Ile Gly 
            100                 105                 110         


Ala Thr Val Phe Ser Ser Thr Ser Phe Ala Asp Lys Arg Ile Gly Ser 
        115                 120                 125             


Ala Phe Gln Phe Gly Asp His Ile Gly Leu Gly Phe Leu Leu Thr Pro 
    130                 135                 140                 


Ser Asn Arg Ile Gly Leu Arg Tyr Ser His Phe Ser Asn Ala Gly Ile 
145                 150                 155                 160 


Lys Glu Pro Asn Pro Gly Leu Asp Ile Val Gln Leu Thr Tyr Thr Tyr 
                165                 170                 175     


Gln Phe 
        


<210>  8
<211>  187
<212>  PRT
<213>  Salmonella typhimurium

<400>  8

Met Tyr Met Lys Arg Ile Phe Ile Tyr Leu Leu Leu Pro Cys Ala Phe 
1               5                   10                  15      


Ala Cys Ser Ala Asn Asp Asn Val Phe Phe Gly Lys Gly Asn Lys His 
            20                  25                  30          


Gln Ile Ser Phe Ala Ala Gly Glu Ser Ile Arg Arg Gly Gly Val Glu 
        35                  40                  45              


His Leu Tyr Thr Ala Phe Leu Thr Tyr Ser Glu Pro Ser Asp Phe Phe 
    50                  55                  60                  


Phe Leu Gln Ala Arg Asn Asn Leu Glu Leu Gly Gly Phe Lys Ala Lys 
65                  70                  75                  80  


Gly Ser Asp Asp Cys Ser Lys His Ser Gly Ser Val Pro Cys Asn Lys 
                85                  90                  95      


Tyr Asn Gln Gly Val Leu Gly Ile Ser Lys Asp Val Ala Leu Val His 
            100                 105                 110         


Phe Ala Gly Ile Tyr Thr Gly Ile Gly Leu Gly Ala Tyr Ile Lys Ser 
        115                 120                 125             


Lys Ser Arg Asp Asp Met Arg Val Asn Ser Ala Phe Thr Phe Gly Glu 
    130                 135                 140                 


Lys Ala Phe Leu Gly Trp Asn Phe Gly Ala Phe Ser Thr Glu Ala Tyr 
145                 150                 155                 160 


Ile Arg His Phe Ser Asn Gly Ser Leu Thr Asp Lys Asn Ser Gly His 
                165                 170                 175     


Asn Phe Val Gly Ala Ser Ile Ser Tyr Asn Phe 
            180                 185         


<210>  9
<211>  173
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  9

Met Lys Lys Leu Leu Pro Leu Ala Val Leu Ala Ala Leu Ser Ser Val 
1               5                   10                  15      


His Val Ala Ser Ala Gln Ala Ala Asp Val Ser Ala Ala Val Gly Ala 
            20                  25                  30          


Thr Gly Gln Ser Gly Met Thr Tyr Arg Leu Gly Leu Ser Trp Asp Trp 
        35                  40                  45              


Asp Lys Ser Trp Trp Gln Thr Ser Thr Gly Arg Leu Thr Gly Tyr Trp 
    50                  55                  60                  


Asp Ala Gly Tyr Thr Tyr Trp Glu Gly Gly Asp Glu Gly Ala Gly Lys 
65                  70                  75                  80  


His Ser Leu Ser Phe Ala Pro Val Phe Val Tyr Glu Phe Ala Gly Asp 
                85                  90                  95      


Ser Ile Lys Pro Phe Ile Glu Ala Gly Ile Gly Val Ala Ala Phe Ser 
            100                 105                 110         


Gly Thr Arg Val Gly Asp Gln Asn Leu Gly Ser Ser Leu Asn Phe Glu 
        115                 120                 125             


Asp Arg Ile Gly Ala Gly Leu Lys Phe Ala Asn Gly Gln Ser Val Gly 
    130                 135                 140                 


Val Arg Ala Ile His Tyr Ser Asn Ala Gly Leu Lys Gln Pro Asn Asp 
145                 150                 155                 160 


Gly Ile Glu Ser Tyr Ser Leu Phe Tyr Lys Ile Pro Ile 
                165                 170             


<210>  10
<211>  172
<212>  PRT
<213>  Pseudomonas fluorescens

<400>  10

Met Lys Arg Leu Phe Cys Leu Ala Ala Ile Ala Ala Ala Met Leu Gly 
1               5                   10                  15      


His Ser Val Ser Ala Gln Ala Ala Gly Leu Glu Phe Gly Leu Gly Ser 
            20                  25                  30          


Thr Ser Asp Ser Thr Leu Thr Tyr Arg Leu Gly Leu Thr Ser Asp Trp 
        35                  40                  45              


Asp Lys Ser Trp Met Gln Ser Asn Val Gly Arg Leu Thr Gly Tyr Trp 
    50                  55                  60                  


Ser Gly Ala Tyr Thr Tyr Trp Glu Gly Asp Asp Arg Ala Gly Ala Ser 
65                  70                  75                  80  


Ser Leu Ser Phe Ser Pro Val Phe Val Tyr Glu Phe Ala Gly Glu Ser 
                85                  90                  95      


Val Lys Pro Tyr Ile Glu Ala Gly Ile Gly Val Ala Leu Phe Ser Arg 
            100                 105                 110         


Thr Arg Leu Glu Asp Asn Asn Ile Gly Gln Ser Phe Gln Phe Glu Asp 
        115                 120                 125             


Arg Leu Gly Phe Gly Leu Arg Phe Asn Gly Gly His Glu Val Gly Ile 
    130                 135                 140                 


Arg Ala Thr His Tyr Ser Asn Ala Gly Ile Ser Ser Asn Asn Asp Gly 
145                 150                 155                 160 


Val Glu Ser Tyr Ser Leu His Tyr Thr Met Pro Leu 
                165                 170         


<210>  11
<211>  172
<212>  PRT
<213>  Pseudomonas putida

<400>  11

Met Arg Lys Leu Leu Gly Leu Ala Ala Ala Ala Ala Phe Thr Leu Gly 
1               5                   10                  15      


Gln Ala Met Ser Ala Gln Ala Ala Asp Val Ser Phe Ser Val Gly Gln 
            20                  25                  30          


Thr Gly Asp Ser Thr Met Val Tyr Arg Leu Gly Leu Gln Ser Asn Trp 
        35                  40                  45              


Asp Ala Ser Trp Trp Gln Thr Ser Val Gly Arg Leu Thr Gly Tyr Trp 
    50                  55                  60                  


Asp Gly Ala Tyr Thr Tyr Trp Asp Gly Asp Glu Thr Ala Ser Asn His 
65                  70                  75                  80  


Ser Leu Ser Phe Ala Pro Val Phe Val Tyr Glu Phe Ala Gly Glu Ser 
                85                  90                  95      


Val Lys Pro Tyr Ile Glu Ala Gly Ile Gly Val Ala Ala Phe Ser Ser 
            100                 105                 110         


Thr Glu Leu Glu Ser Asn Glu Leu Gly Ser Ser Phe Gln Phe Glu Asp 
        115                 120                 125             


Arg Ile Gly Phe Gly Leu Arg Phe Ala Gly Gly His Glu Ile Gly Val 
    130                 135                 140                 


Arg Ala Ile His Tyr Ser Asn Ala Gly Ile Lys Glu Pro Asn Asp Gly 
145                 150                 155                 160 


Val Glu Ser Tyr Ser Leu His Tyr Arg Met Ala Leu 
                165                 170         


<210>  12
<211>  172
<212>  PRT
<213>  Pseudomonas syringae

<400>  12

Met Lys Arg Leu Phe Cys Leu Ala Ala Ile Ala Ala Ala Leu Leu Gly 
1               5                   10                  15      


Gln Thr Ser Leu Ser Gln Ala Ala Gly Val Glu Phe Ser Val Gly Gln 
            20                  25                  30          


Thr Gly Gln Thr Thr Gln Thr Tyr Arg Leu Gly Met Lys Phe Asp Trp 
        35                  40                  45              


Asp Gln Ser Trp Leu Gln Ser Asp Val Gly Arg Leu Thr Gly Tyr Trp 
    50                  55                  60                  


Asp Gly Ala Tyr Thr Tyr Trp Asp Gly Asp Lys Gly Ser Ser Asn Asn 
65                  70                  75                  80  


Ser Leu Ser Phe Ser Pro Val Leu Val Tyr Glu Phe Ala Gly Glu Thr 
                85                  90                  95      


Val Lys Pro Tyr Val Glu Ala Gly Ile Gly Val Ala Val Phe Ser Asn 
            100                 105                 110         


Thr Lys Tyr Glu Asp Lys Asn Phe Gly Ser Ala Phe Asn Phe Glu Asp 
        115                 120                 125             


Arg Ile Gly Phe Gly Leu Arg Phe Asn Gly Gly His Glu Ile Gly Ile 
    130                 135                 140                 


Arg Ala Thr His Tyr Ser Asn Ala Gly Ile Lys Gln Pro Asn Asp Gly 
145                 150                 155                 160 


Ile Glu Ser Tyr Ala Leu His Tyr Thr Met Pro Leu 
                165                 170         


<210>  13
<211>  187
<212>  PRT
<213>  Burkholderia sp.

<400>  13

Met Lys Asn Lys Lys Asn Ala Leu Arg Gly Leu Thr Val Lys Gly Val 
1               5                   10                  15      


Ser Ala Ala Ile Leu Leu Ala Ala Ser Gly Leu Ala Ala Ala Asp Gln 
            20                  25                  30          


Phe Gly Val Gln Ile Ala Gly Gly Val Gly Asp His His Ile Lys Lys 
        35                  40                  45              


Leu Asp Leu Gly Phe Val Trp Asp Pro Asn Leu Thr Trp Trp Gln Ile 
    50                  55                  60                  


Gly Asp Trp His Phe Ala Leu Ile Gly Glu Ala His Val Ala Trp Trp 
65                  70                  75                  80  


His Thr Asn Glu Gly Asn Val His Glu Asn Ile Gly Glu Ile Gly Val 
                85                  90                  95      


Thr Pro Ile Ile Arg Phe Ile Lys Ala Ser Gly Pro Ile Arg Pro Tyr 
            100                 105                 110         


Val Glu Ala Gly Ala Gly Val Arg Leu Leu Thr Ser Pro Arg Ile Ser 
        115                 120                 125             


Ser Asp Leu Thr Leu Ala Thr Ala Phe Gln Phe Ala Pro Met Ala Gly 
    130                 135                 140                 


Val Gly Leu Gln Phe Gly Ser His Gln Gln Tyr Glu Ala Gly Tyr Arg 
145                 150                 155                 160 


Phe Gln His Ile Ser Asn Gly Gly Ile Lys Glu Pro Asn Pro Gly Ile 
                165                 170                 175     


Asn Phe His Gln Leu Tyr Leu Gln Tyr Asn Phe 
            180                 185         


<210>  14
<211>  187
<212>  PRT
<213>  Ralstonia solanacearum

<400>  14

Met Ile Arg Ser Ala Leu Pro Arg Ser Ala Lys Pro Leu Ala Ala Ala 
1               5                   10                  15      


Leu Ala Ala Ala Thr Leu Leu Ala Ala Ala Pro Ala Gln Ala Asp Pro 
            20                  25                  30          


Ser Val Arg Ala Ile Tyr Gly Arg Asp Asn Arg His Gly Ile Glu Lys 
        35                  40                  45              


Tyr Gly Val Asp Ile Asp Phe Asp Ser Gly Phe His Trp Gly Asn Pro 
    50                  55                  60                  


Gln Gly Trp Phe Leu Asn Leu Asp Trp Glu Ile Ala Leu Gly Gln Trp 
65                  70                  75                  80  


Arg Ser Thr Lys Gly Thr Asn Arg Gln Asn Leu Thr Glu Phe Gly Val 
                85                  90                  95      


Thr Pro Leu Phe Arg Leu Glu Lys Arg Gly Gly Ser Trp Val Pro Phe 
            100                 105                 110         


Ile Glu Ala Gly Ile Gly Pro Arg Leu Leu Ser His Thr Arg Thr Ser 
        115                 120                 125             


Asp Glu His Asn Phe Ser Thr Ala Phe Gln Phe Ser Asp Met Ile Gly 
    130                 135                 140                 


Val Gly Val Ala Phe Gly Ser Arg Gln Gln Phe Gln Val Gly Tyr Arg 
145                 150                 155                 160 


Phe Glu His Leu Ser Asn Ala Ser Ile Lys Arg Pro Asn Pro Gly Thr 
                165                 170                 175     


Asp Leu Asn Glu Leu Tyr Leu Arg Tyr Thr Phe 
            180                 185         


<210>  15
<211>  177
<212>  PRT
<213>  Azotobacter vinelandii

<400>  15

Met Arg Lys Tyr Leu Ser Leu Pro Ala Val Ala Val Leu Leu Leu Gly 
1               5                   10                  15      


Ser Ala Gly Val Ala Gln Ala Val Glu Val Gly Ala Ala Val Gly Val 
            20                  25                  30          


Thr Ser Gln Asn Asp Met Thr Tyr Arg Leu Ser Leu Gly Leu Pro Trp 
        35                  40                  45              


Glu Lys Gln Trp Trp Lys Ser Asp Leu Gly Tyr Val Thr Gly Tyr Trp 
    50                  55                  60                  


Asp Ala Gly Tyr Thr Tyr Trp Glu Gly Gly Ser Gly Asn Asp Asn Tyr 
65                  70                  75                  80  


Ala Gly Ala His Ser Leu Ser Phe Ser Pro Val Phe Thr Tyr Glu Phe 
                85                  90                  95      


Ser Gly Phe Ser Ser Val Thr Pro Phe Leu Glu Leu Gly Val Gly Val 
            100                 105                 110         


Ala Phe Phe Ser Lys Thr Arg Val Gly Glu Gln Gln Leu Gly Ser Ser 
        115                 120                 125             


Phe Asn Phe Glu Asp Arg Ile Gly Ala Gly Ile Lys Phe Ala Gly Gly 
    130                 135                 140                 


Gln Lys Val Gly Ile Arg Ala Ile His Tyr Ser Asn Ala Gly Ile Lys 
145                 150                 155                 160 


Gln Pro Asn Asp Gly Ile Glu Ser Phe Ser Ala Tyr Tyr Ser His Ala 
                165                 170                 175     


Phe 
    


<210>  16
<211>  191
<212>  PRT
<213>  Rhizobium etli

<400>  16

Met Ser Ala Asp Phe Gly Ser Ile Val Leu Arg Phe Leu Thr Thr Ala 
1               5                   10                  15      


Leu Leu Ala Ala Val Ala Gly Thr Val Ala Gly Ser Ala Ser Ala Gly 
            20                  25                  30          


Glu Gln Ile Phe Asp Glu Leu Arg Phe Gly Val Ser Thr Ser Leu Gln 
        35                  40                  45              


Ser Gly His Ser Arg Glu Asp Gly Val Phe Pro Glu Ile Thr Ala Leu 
    50                  55                  60                  


Phe Asp Pro Phe Gly Tyr Glu Gln Ala Val Gly Trp Gln Gln Gln Leu 
65                  70                  75                  80  


Leu Arg Pro Arg Val His Leu Gly Thr Ser Ile Gly Thr Ala Gly Glu 
                85                  90                  95      


Ala Thr Gln Phe Phe Thr Gly Phe Thr Trp Thr Val Asp Leu Asn Glu 
            100                 105                 110         


Lys Leu Phe Ala Glu Ala Gly Phe Gly Gly Val Ile His Thr Gly Asp 
        115                 120                 125             


Leu Asp Asn Asn Asp Asp Gly Pro Asp Leu Gly Cys Arg Val Leu Phe 
    130                 135                 140                 


His Glu Tyr Ala Gly Ala Gly Tyr Arg Phe Thr Pro His Trp Asn Val 
145                 150                 155                 160 


Met Ala Gln Ile Ala His Ser Ser His Ala Asn Leu Cys Asp Gly Pro 
                165                 170                 175     


Asn Asp Gly Met Thr Arg Ala Gly Ile Gln Ile Gly Tyr Lys Phe 
            180                 185                 190     


<210>  17
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  LptA Fw primer

<400>  17
gccttccttt ccctgtattc                                                   20


<210>  18
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  LptA Re primer

<400>  18
ggtgttcgga cacatatgc                                                    19


<210>  19
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  LpxL1 Fw primer

<400>  19
ctgatcgggc agatacag                                                     18


<210>  20
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  LpxL1 Re primer

<400>  20
gtgcgctacc gcaataag                                                     18


<210>  21
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  LpxL2 Fw primer

<400>  21
aaacagatac tgcgtcggaa                                                   20


<210>  22
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  LpxL2 Re primer

<400>  22
ccctttgcga accgccat                                                     18


<210>  23
<211>  15
<212>  DNA
<213>  Artificial

<220>
<223>  PagL Fw primer

<400>  23
atgcaatttc tcaag                                                        15


<210>  24
<211>  15
<212>  DNA
<213>  Artificial

<220>
<223>  PagL Re primer

<400>  24
tcagaactgg tacgt                                                        15


<210>  25
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  LpxP Fw primer

<400>  25
catatggccg cttacgcaga caatacac                                          28


<210>  26
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  LpxP Re primer

<400>  26
gacgtcacgc ctgaatgact tcattacacc                                        30


<210>  27
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  LpxE Fw primer

<400>  27
catatgatcc ggccctcatc ccattccc                                          28


<210>  28
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  LpxE Re primer

<400>  28
tcatgacccg aaaggcgctt cccttcag                                          28


