                         SEQUENCE LISTING

<110>  Universitt Bern
 
<120>  Nanospheres of SEC-14 Like Proteins and its Cognate Ligands

<130>  P5060PC00

<150>  EP16152579.5
<151>  2016-01-25

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  1
gggaattcgc agaggcgcga tcccag                                            26


<210>  2
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  2
ccgtcattga atgctctcag aaatgc                                            26


<210>  3
<211>  278
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ala Glu Ala Arg Ser Gln Pro Ser Ala Gly Pro Gln Leu Asn Ala 
1               5                   10                  15      


Leu Pro Asp His Ser Pro Leu Leu Gln Pro Gly Leu Ala Ala Leu Arg 
            20                  25                  30          


Arg Arg Ala Arg Glu Ala Gly Val Pro Leu Ala Pro Leu Pro Leu Thr 
        35                  40                  45              


Asp Ser Phe Leu Leu Arg Phe Leu Arg Ala Arg Asp Phe Asp Leu Asp 
    50                  55                  60                  


Leu Ala Trp Arg Leu Leu Lys Asn Tyr Tyr Lys Trp Arg Ala Glu Cys 
65                  70                  75                  80  


Pro Glu Ile Ser Ala Asp Leu His Pro Arg Ser Ile Ile Gly Leu Leu 
                85                  90                  95      


Lys Ala Gly Tyr His Gly Val Leu Arg Ser Arg Asp Pro Thr Gly Ser 
            100                 105                 110         


Lys Val Leu Ile Tyr Arg Ile Ala His Trp Asp Pro Lys Val Phe Thr 
        115                 120                 125             


Ala Tyr Asp Val Phe Arg Val Ser Leu Ile Thr Ser Glu Leu Ile Val 
    130                 135                 140                 


Gln Glu Val Glu Thr Gln Arg Asn Gly Ile Lys Ala Ile Phe Asp Leu 
145                 150                 155                 160 


Glu Gly Trp Gln Phe Ser His Ala Phe Gln Ile Thr Pro Ser Val Ala 
                165                 170                 175     


Lys Lys Ile Ala Ala Val Leu Thr Asp Ser Phe Pro Leu Lys Val Arg 
            180                 185                 190         


Gly Ile His Leu Ile Asn Glu Pro Val Ile Phe His Ala Val Phe Ser 
        195                 200                 205             


Met Ile Lys Pro Phe Leu Thr Glu Lys Ile Lys Glu Arg Ile His Met 
    210                 215                 220                 


His Gly Asn Asn Tyr Lys Gln Ser Leu Leu Gln His Phe Pro Asp Ile 
225                 230                 235                 240 


Leu Pro Leu Glu Tyr Gly Gly Glu Glu Phe Ser Met Glu Asp Ile Cys 
                245                 250                 255     


Gln Glu Trp Thr Asn Phe Ile Met Lys Ser Glu Asp Tyr Leu Ser Ser 
            260                 265                 270         


Ile Ser Glu Ser Ile Gln 
        275             


<210>  4
<211>  317
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Ser Glu Gly Val Gly Thr Phe Arg Met Val Pro Glu Glu Glu Gln 
1               5                   10                  15      


Glu Leu Arg Ala Gln Leu Glu Gln Leu Thr Thr Lys Asp His Gly Pro 
            20                  25                  30          


Val Phe Gly Pro Cys Ser Gln Leu Pro Arg His Thr Leu Gln Lys Ala 
        35                  40                  45              


Lys Asp Glu Leu Asn Glu Arg Glu Glu Thr Arg Glu Glu Ala Val Arg 
    50                  55                  60                  


Glu Leu Gln Glu Met Val Gln Ala Gln Ala Ala Ser Gly Glu Glu Leu 
65                  70                  75                  80  


Ala Val Ala Val Ala Glu Arg Val Gln Glu Lys Asp Ser Gly Phe Phe 
                85                  90                  95      


Leu Arg Phe Ile Arg Ala Arg Lys Phe Asn Val Gly Arg Ala Tyr Glu 
            100                 105                 110         


Leu Leu Arg Gly Tyr Val Asn Phe Arg Leu Gln Tyr Pro Glu Leu Phe 
        115                 120                 125             


Asp Ser Leu Ser Pro Glu Ala Val Arg Cys Thr Ile Glu Ala Gly Tyr 
    130                 135                 140                 


Pro Gly Val Leu Ser Ser Arg Asp Lys Tyr Gly Arg Val Val Met Leu 
145                 150                 155                 160 


Phe Asn Ile Glu Asn Trp Gln Ser Gln Glu Ile Thr Phe Asp Glu Ile 
                165                 170                 175     


Leu Gln Ala Tyr Cys Phe Ile Leu Glu Lys Leu Leu Glu Asn Glu Glu 
            180                 185                 190         


Thr Gln Ile Asn Gly Phe Cys Ile Ile Glu Asn Phe Lys Gly Phe Thr 
        195                 200                 205             


Met Gln Gln Ala Ala Ser Leu Arg Thr Ser Asp Leu Arg Lys Met Val 
    210                 215                 220                 


Asp Met Leu Gln Asp Ser Phe Pro Ala Arg Phe Lys Ala Ile His Phe 
225                 230                 235                 240 


Ile His Gln Pro Trp Tyr Phe Thr Thr Thr Tyr Asn Val Val Lys Pro 
                245                 250                 255     


Phe Leu Lys Ser Lys Leu Leu Glu Arg Val Phe Val His Gly Asp Asp 
            260                 265                 270         


Leu Ser Gly Phe Tyr Gln Glu Ile Asp Glu Asn Ile Leu Pro Ser Asp 
        275                 280                 285             


Phe Gly Gly Thr Leu Pro Lys Tyr Asp Gly Lys Ala Val Ala Glu Gln 
    290                 295                 300                 


Leu Phe Gly Pro Gln Ala Gln Ala Glu Asn Thr Ala Phe 
305                 310                 315         


<210>  5
<211>  354
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Gly Pro Val Ser Leu Leu Pro Lys Tyr Gln Lys Leu Asn Thr Trp 
1               5                   10                  15      


Asn Gly Asp Leu Ala Lys Met Thr His Leu Gln Ala Gly Leu Ser Pro 
            20                  25                  30          


Glu Thr Ile Glu Lys Ala Arg Leu Glu Leu Asn Glu Asn Pro Asp Val 
        35                  40                  45              


Leu His Gln Asp Ile Gln Gln Val Arg Asp Met Ile Ile Thr Arg Pro 
    50                  55                  60                  


Asp Ile Gly Phe Leu Arg Thr Asp Asp Ala Phe Ile Leu Arg Phe Leu 
65                  70                  75                  80  


Arg Ala Arg Lys Phe His Gln Ala Asp Ala Phe Arg Leu Leu Ala Gln 
                85                  90                  95      


Tyr Phe Gln Tyr Arg Gln Leu Asn Leu Asp Met Phe Lys Asn Phe Lys 
            100                 105                 110         


Ala Asp Asp Pro Gly Ile Lys Arg Ala Leu Ile Asp Gly Phe Pro Gly 
        115                 120                 125             


Val Leu Glu Asn Arg Asp His Tyr Gly Arg Lys Ile Leu Leu Leu Phe 
    130                 135                 140                 


Ala Ala Asn Trp Asp Gln Ser Arg Asn Ser Phe Thr Asp Ile Leu Arg 
145                 150                 155                 160 


Ala Ile Leu Leu Ser Leu Glu Val Leu Ile Glu Asp Pro Glu Leu Gln 
                165                 170                 175     


Ile Asn Gly Phe Ile Leu Ile Ile Asp Trp Ser Asn Phe Ser Phe Lys 
            180                 185                 190         


Gln Ala Ser Lys Leu Thr Pro Ser Ile Leu Lys Leu Ala Ile Glu Gly 
        195                 200                 205             


Leu Gln Asp Ser Phe Pro Ala Arg Phe Gly Gly Val His Phe Val Asn 
    210                 215                 220                 


Gln Pro Trp Tyr Ile His Ala Leu Tyr Thr Leu Ile Lys Pro Phe Leu 
225                 230                 235                 240 


Lys Asp Lys Thr Arg Lys Arg Ile Phe Leu His Gly Asn Asn Leu Asn 
                245                 250                 255     


Ser Leu His Gln Leu Ile His Pro Glu Phe Leu Pro Ser Glu Phe Gly 
            260                 265                 270         


Gly Thr Leu Pro Pro Tyr Asp Met Gly Thr Trp Ala Arg Thr Leu Leu 
        275                 280                 285             


Gly Pro Asp Tyr Ser Asp Glu Asn Asp Tyr Thr His Thr Ser Tyr Asn 
    290                 295                 300                 


Ala Met His Val Lys His Thr Ser Ser Asn Leu Glu Arg Glu Cys Ser 
305                 310                 315                 320 


Pro Lys Leu Met Lys Arg Ser Gln Ser Val Val Glu Ala Gly Thr Leu 
                325                 330                 335     


Lys His Glu Glu Lys Gly Glu Asn Glu Asn Thr Gln Pro Leu Leu Ala 
            340                 345                 350         


Leu Asp 
        


<210>  6
<211>  327
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Thr His Leu Gln Ala Gly Leu Ser Pro Glu Thr Leu Glu Lys Ala 
1               5                   10                  15      


Arg Leu Glu Leu Asn Glu Asn Pro Asp Thr Leu His Gln Asp Ile Gln 
            20                  25                  30          


Glu Val Arg Asp Met Val Ile Thr Arg Pro Asp Ile Gly Phe Leu Arg 
        35                  40                  45              


Thr Asp Asp Ala Phe Ile Leu Arg Phe Leu Arg Ala Arg Lys Phe His 
    50                  55                  60                  


His Phe Glu Ala Phe Arg Leu Leu Ala Gln Tyr Phe Glu Tyr Arg Gln 
65                  70                  75                  80  


Gln Asn Leu Asp Met Phe Lys Ser Phe Lys Ala Thr Asp Pro Gly Ile 
                85                  90                  95      


Lys Gln Ala Leu Lys Asp Gly Phe Pro Gly Gly Leu Ala Asn Leu Asp 
            100                 105                 110         


His Tyr Gly Arg Lys Ile Leu Val Leu Phe Ala Ala Asn Trp Asp Gln 
        115                 120                 125             


Ser Arg Tyr Thr Leu Val Asp Ile Leu Arg Ala Ile Leu Leu Ser Leu 
    130                 135                 140                 


Glu Ala Met Ile Glu Asp Pro Glu Leu Gln Val Asn Gly Phe Val Leu 
145                 150                 155                 160 


Ile Ile Asp Trp Ser Asn Phe Thr Phe Lys Gln Ala Ser Lys Leu Thr 
                165                 170                 175     


Pro Ser Met Leu Arg Leu Ala Ile Glu Gly Leu Gln Asp Ser Phe Pro 
            180                 185                 190         


Ala Arg Phe Gly Gly Ile His Phe Val Asn Gln Pro Trp Tyr Ile His 
        195                 200                 205             


Ala Leu Tyr Thr Val Ile Arg Pro Phe Leu Lys Glu Lys Thr Arg Lys 
    210                 215                 220                 


Arg Ile Phe Leu His Gly Asn Asn Leu Asn Ser Leu His Gln Leu Ile 
225                 230                 235                 240 


His Pro Glu Ile Leu Pro Ser Glu Phe Gly Gly Met Leu Pro Pro Tyr 
                245                 250                 255     


Asp Met Gly Thr Trp Ala Arg Thr Leu Leu Asp His Glu Tyr Asp Asp 
            260                 265                 270         


Asp Ser Glu Tyr Asn Val Asp Ser Tyr Ser Met Pro Val Lys Glu Val 
        275                 280                 285             


Glu Lys Glu Leu Ser Pro Lys Ser Met Lys Arg Ser Gln Ser Val Val 
    290                 295                 300                 


Asp Pro Thr Val Leu Lys Arg Met Asp Lys Asn Glu Glu Glu Asn Met 
305                 310                 315                 320 


Gln Pro Leu Leu Ser Leu Asp 
                325         


<210>  7
<211>  342
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Ser Glu Glu Ser Asp Ser Leu Arg Thr Ser Pro Ser Val Ala Ser 
1               5                   10                  15      


Leu Ser Glu Asn Glu Leu Pro Pro Pro Pro Glu Pro Pro Gly Tyr Val 
            20                  25                  30          


Cys Ser Leu Thr Glu Asp Leu Val Thr Lys Ala Arg Glu Glu Leu Gln 
        35                  40                  45              


Glu Lys Pro Glu Trp Arg Leu Arg Asp Val Gln Ala Leu Arg Asp Met 
    50                  55                  60                  


Val Arg Lys Glu Tyr Pro Asn Leu Ser Thr Ser Leu Asp Asp Ala Phe 
65                  70                  75                  80  


Leu Leu Arg Phe Leu Arg Ala Arg Lys Phe Asp Tyr Asp Arg Ala Leu 
                85                  90                  95      


Gln Leu Leu Val Asn Tyr His Ser Cys Arg Arg Ser Trp Pro Glu Val 
            100                 105                 110         


Phe Asn Asn Leu Lys Pro Ser Ala Leu Lys Asp Val Leu Ala Ser Gly 
        115                 120                 125             


Phe Leu Thr Val Leu Pro His Thr Asp Pro Arg Gly Cys His Val Val 
    130                 135                 140                 


Cys Ile Arg Pro Asp Arg Trp Ile Pro Ser Asn Tyr Pro Ile Thr Glu 
145                 150                 155                 160 


Asn Ile Arg Ala Ile Tyr Leu Thr Leu Glu Lys Leu Ile Gln Ser Glu 
                165                 170                 175     


Glu Thr Gln Val Asn Gly Ile Val Ile Leu Ala Asp Tyr Lys Gly Val 
            180                 185                 190         


Ser Leu Ser Lys Ala Ser His Phe Gly Pro Phe Ile Ala Lys Lys Val 
        195                 200                 205             


Ile Gly Ile Leu Gln Asp Gly Phe Pro Ile Arg Ile Lys Ala Val His 
    210                 215                 220                 


Val Val Asn Glu Pro Arg Ile Phe Lys Gly Ile Phe Ala Ile Ile Lys 
225                 230                 235                 240 


Pro Phe Leu Lys Glu Lys Ile Ala Asn Arg Phe Phe Leu His Gly Ser 
                245                 250                 255     


Asp Leu Asn Ser Leu His Thr Asn Leu Pro Arg Ser Ile Leu Pro Lys 
            260                 265                 270         


Glu Tyr Gly Gly Thr Ala Gly Glu Leu Asp Thr Ala Thr Trp Asn Ala 
        275                 280                 285             


Val Leu Leu Ala Ser Glu Asp Asp Phe Val Lys Glu Phe Cys Gln Pro 
    290                 295                 300                 


Val Pro Ala Cys Asp Ser Ile Leu Gly Gln Thr Leu Leu Pro Glu Gly 
305                 310                 315                 320 


Leu Thr Ser Asp Ala Gln Cys Asp Asp Ser Leu Arg Ala Val Lys Ser 
                325                 330                 335     


Gln Leu Tyr Ser Cys Tyr 
            340         


<210>  8
<211>  19
<212>  PRT
<213>  artificial sequence

<220>
<223>  FIG. 8A - alpha TTP


<220>
<221>  misc_feature
<222>  (2)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (9)..(11)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (16)..(18)
<223>  Xaa can be any naturally occurring amino acid

<400>  8

Leu Xaa Xaa Xaa Xaa Phe Xaa Leu Xaa Xaa Xaa Trp Xaa Xaa Leu Xaa 
1               5                   10                  15      


Xaa Xaa Tyr 
            


<210>  9
<211>  19
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fig 8A CRALBP


<220>
<221>  misc_feature
<222>  (2)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (9)..(11)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (16)..(18)
<223>  Xaa can be any naturally occurring amino acid

<400>  9

Ile Xaa Xaa Xaa Xaa Phe Xaa Val Xaa Xaa Xaa Tyr Xaa Xaa Leu Xaa 
1               5                   10                  15      


Xaa Xaa Val 
            


<210>  10
<211>  44
<212>  PRT
<213>  artificial sequence

<220>
<223>  FIG. 8B - a-TTP aa (47-90)

<400>  10

Leu Thr Asp Ser Phe Leu Leu Arg Phe Leu Arg Ala Arg Asp Phe Asp 
1               5                   10                  15      


Leu Asp Leu Ala Trp Arg Leu Leu Lys Asn Tyr Tyr Lys Trp Arg Ala 
            20                  25                  30          


Glu Cys Pro Glu Ile Ser Ala Asp Leu His Pro Arg 
        35                  40                  


<210>  11
<211>  44
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fig. 8B - CRALBP aa (91-134)

<400>  11

Lys Asp Ser Gly Phe Phe Leu Arg Phe Ile Arg Ala Arg Lys Phe Asn 
1               5                   10                  15      


Val Gly Arg Ala Tyr Glu Leu Leu Arg Gly Tyr Val Asn Phe Arg Leu 
            20                  25                  30          


Gln Tyr Pro Glu Leu Phe Asp Ser Leu Ser Pro Glu 
        35                  40                  


<210>  12
<211>  44
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fig. 8B - CLVS1 aa (71-114)

<400>  12

Thr Asp Asp Ala Phe Ile Leu Arg Phe Leu Arg Ala Arg Lys Phe His 
1               5                   10                  15      


Gln Ala Asp Ala Phe Arg Leu Leu Ala Gln Tyr Phe Gln Tyr Arg Gln 
            20                  25                  30          


Leu Asn Leu Asp Met Phe Lys Asn Phe Lys Ala Asp 
        35                  40                  


<210>  13
<211>  44
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fig 8B - CLVS2 aa (49-92)

<400>  13

Thr Asp Asp Ala Phe Ile Leu Arg Phe Leu Arg Ala Arg Lys Phe His 
1               5                   10                  15      


His Phe Glu Ala Phe Arg Leu Leu Ala Gln Tyr Phe Glu Tyr Arg Gln 
            20                  25                  30          


Gln Asn Leu Asp Met Phe Lys Ser Phe Lys Ala Thr 
        35                  40                  


<210>  14
<211>  44
<212>  PRT
<213>  artificial sequence

<220>
<223>  FIG. 8B - TTPAL aa (76-119)

<400>  14

Leu Asp Asp Ala Phe Leu Leu Arg Phe Leu Arg Ala Arg Lys Phe Asp 
1               5                   10                  15      


Tyr Asp Arg Ala Leu Gln Leu Leu Val Asn Tyr His Ser Cys Arg Arg 
            20                  25                  30          


Ser Trp Pro Glu Val Phe Asn Asn Leu Lys Pro Ser 
        35                  40                  


<210>  15
<211>  34
<212>  PRT
<213>  artificial sequence

<220>
<223>  FIG 8C - a-TTP aa (47-80)

<400>  15

Leu Thr Asp Ser Phe Leu Leu Arg Phe Leu Arg Ala Arg Asp Phe Asp 
1               5                   10                  15      


Leu Asp Leu Ala Trp Arg Leu Leu Lys Asn Tyr Tyr Lys Trp Arg Ala 
            20                  25                  30          


Glu Cys 
        


<210>  16
<211>  34
<212>  PRT
<213>  artificial sequence

<220>
<223>  FIG. 8C - CRALBP aa (91-124)

<400>  16

Lys Asp Ser Gly Phe Phe Leu Arg Phe Ile Arg Ala Arg Lys Phe Asn 
1               5                   10                  15      


Val Gly Arg Ala Tyr Glu Leu Leu Arg Gly Tyr Val Asn Phe Arg Leu 
            20                  25                  30          


Gln Tyr 
        


