                         SEQUENCE LISTING

<110>  Academisch Ziekenhuis Leiden (also acting under the name of
        Leiden University Medical)
 
<120>  INSULIN GENE-DERIVED PROTEINS AND PEPTIDES FOR USE IN THE 
       DIAGNOSIS AND TREATMENT OF TYPE 1 DIABETES

<130>  1420.004 WO

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  469
<212>  RNA
<213>  wild type human insulin gene

<400>  1
agcccuccag gacaggcugc aucagaagag gccaucaagc agaucacugu ccuucugcca       60

uggcccugug gaugcgccuc cugccccugc uggcgcugcu ggcccucugg ggaccugacc      120

cagccgcagc cuuugugaac caacaccugu gcggcucaca ccugguggaa gcucucuacc      180

uagugugcgg ggaacgaggc uucuucuaca cacccaagac ccgccgggag gcagaggacc      240

ugcagguggg gcagguggag cugggcgggg gcccuggugc aggcagccug cagcccuugg      300

cccuggaggg gucccugcag aagcguggca uuguggaaca augcuguacc agcaucugcu      360

cccucuacca gcuggagaac uacugcaacu agacgcagcc cgcaggcagc cccacacccg      420

ccgccuccug caccgagaga gauggaauaa agcccuugaa ccagcaaaa                  469


<210>  2
<211>  110
<212>  PRT
<213>  protein sequence of PPI

<400>  2

Met Ala Leu Trp Met Arg Leu Leu Pro Leu Leu Ala Leu Leu Ala Leu 
1               5                   10                  15      


Trp Gly Pro Asp Pro Ala Ala Ala Phe Val Asn Gln His Leu Cys Gly 
            20                  25                  30          


Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe 
        35                  40                  45              


Phe Tyr Thr Pro Lys Thr Arg Arg Glu Ala Glu Asp Leu Gln Val Gly 
    50                  55                  60                  


Gln Val Glu Leu Gly Gly Gly Pro Gly Ala Gly Ser Leu Gln Pro Leu 
65                  70                  75                  80  


Ala Leu Glu Gly Ser Leu Gln Lys Arg Gly Ile Val Glu Gln Cys Cys 
                85                  90                  95      


Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn 
            100                 105                 110 


<210>  3
<211>  43
<212>  PRT
<213>  DRiP CH

<400>  3

Met Leu Tyr Gln His Leu Leu Pro Leu Pro Ala Gly Glu Leu Leu Gln 
1               5                   10                  15      


Leu Asp Ala Ala Cys Arg Gln Pro His Thr Arg Arg Leu Leu His Arg 
            20                  25                  30          


Glu Arg Trp Asn Lys Ala Leu Glu Pro Ala Lys 
        35                  40              


<210>  4
<211>  43
<212>  PRT
<213>  DRiP RP

<400>  4

Met Leu Tyr Gln His Leu Leu Pro Leu Pro Ala Gly Glu Leu Leu Gln 
1               5                   10                  15      


Leu Asp Ala Ala Arg Arg Gln Pro Pro Thr Arg Arg Leu Leu His Arg 
            20                  25                  30          


Glu Arg Trp Asn Lys Ala Leu Glu Pro Ala Lys 
        35                  40              


<210>  5
<211>  95
<212>  PRT
<213>  Splice RP

<400>  5

Met Ala Leu Trp Met Arg Leu Leu Pro Leu Leu Ala Leu Leu Ala Leu 
1               5                   10                  15      


Trp Gly Pro Asp Pro Ala Ala Ala Phe Val Asn Gln His Leu Cys Gly 
            20                  25                  30          


Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe 
        35                  40                  45              


Phe Tyr Thr Pro Lys Thr Arg Arg Glu Ala Glu Asp Leu Gln Ala Gly 
    50                  55                  60                  


Glu Leu Leu Gln Leu Asp Ala Ala Arg Arg Gln Pro Pro Thr Arg Arg 
65                  70                  75                  80  


Leu Leu His Arg Glu Arg Trp Asn Lys Ala Leu Glu Pro Ala Lys 
                85                  90                  95  


<210>  6
<211>  18
<212>  PRT
<213>  Unknown or  artificial

<400>  6

Thr Arg Arg Glu Ala Glu Asp Leu Gln Ala Gly Glu Leu Leu Gln Leu 
1               5                   10                  15      


Asp Ala 
        


<210>  7
<211>  14
<212>  PRT
<213>  Epitope in the C-terminal of SEQ IDs 3-5 which has been used to obtain polyclonal antiserum

<400>  7

Leu His Arg Glu Arg Trp Asn Lys Ala Leu Glu Pro Ala Lys 
1               5                   10                  


<210>  8
<211>  9
<212>  PRT
<213>  T-cell epitope in the N-terminal of SEQ IDs 3 and 4

<400>  8

Met Leu Tyr Gln His Leu Leu Pro Leu 
1               5                   


<210>  9
<211>  9
<212>  PRT
<213>  T-cell epitope present in for SEQ IDs 4 and 5

<400>  9

Pro Thr Arg Arg Leu Leu His Arg Glu 
1               5                   


<210>  10
<211>  17
<212>  DNA
<213>  forward primer

<400>  10
agccctccag gacaggc                                                      17


<210>  11
<211>  20
<212>  DNA
<213>  reverse primer

<400>  11
gttgcagtag ttctccagct                                                   20


<210>  12
<211>  24
<212>  DNA
<213>  reverse primer

<400>  12
ttttgctggt tcaagggctt tatt                                              24


