                               SEQUENCE LISTING

<110> THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
 
<120> MULTIPLE STAGE ISOTHERMAL ENZYMATIC AMPLIFICATION

<130> 103241.006169

<140> PCT/US2017/013403
<141> 2017-01-13

<150> 62/278,095
<151> 2016-01-13

<160> 99    

<170> PatentIn version 3.5

<210> 1
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 1
attatcagaa ggagccacc                                                    19


<210> 2
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 2
catcctattt gttcctgaag g                                                 21


<210> 3
<211> 47
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 3
cagcttcctc attgatggtt tctttttaac accatgctaa acacagt                     47


<210> 4
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 4
tgttgcacca ggccagataa ttttgtactg gtagttcctg ctatg                       45


<210> 5
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 5
tttaacattt gcatggctgc ttgat                                             25


<210> 6
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 6
gagatccaag gggaagtga                                                    19


<210> 7
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 7
ttatcgtcta tagtacggta gg                                                22


<210> 8
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 8
atactttaac ccccaccaa                                                    19


<210> 9
<211> 47
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 9
gccaagtaga gactacaaac atctttgggt aaggtagaaa atgttgt                     47


<210> 10
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 10
agaagtgttt aacttgatga aggggaaaca aaaccgaaac cacta                       45


<210> 11
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 11
ctgcacgaaa tacagaat                                                     18


<210> 12
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
gtatgttctg tcctcttg                                                     18


<210> 13
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
tcgcttctaa cggtgaac                                                     18


<210> 14
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
aattgatagt atcagctatc catag                                             25


<210> 15
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
ggtggaacac attgtttcat ttgatctcat tccaatggaa ccttg                       45


<210> 16
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
gtttgcttct aacattccac ttgcccgttt tgaccggtca tt                          42


<210> 17
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 17
cactatacct taccaatcta tttgaacttg                                        30


<210> 18
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 18
tggacgtaac ctccaggc                                                     18


<210> 19
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 19
ctttctaagc ccgcgata                                                     18


<210> 20
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 20
gcgcattaca cttggtct                                                     18


<210> 21
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 21
tacccctaac ttcgtggtct ccccccctta ttttagggtg c                           41


<210> 22
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 22
ctccctatat aacatggcga gtaagactat gaaatcagtg tttttcgg                    48


<210> 23
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 23
ggtgcgcttt gttttccgt                                                    19


<210> 24
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 24
accatgtgta aagcgcgtca aa                                                22


<210> 25
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 25
gcgcataaat tcatcagc                                                     18


<210> 26
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 26
gcaaaactta attacaaaag cg                                                22


<210> 27
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 27
gcttttttta gtagttttgg cacttcttac attagacaag gaaattgg                    48


<210> 28
<211> 46
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 28
gaaaytaatt gactatgtta cgtgcacaac acaatatacg accagc                      46


<210> 29
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 29
aattaraatt aaaattgata aat                                               23


<210> 30
<211> 11
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 30
attgtaccag t                                                            11


<210> 31
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 31
gtgtaggctg gcgtagt                                                      17


<210> 32
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 32
tttcaatttt agcttaggac c                                                 21


<210> 33
<211> 46
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 33
gctactatca ccaagatctg cacgcattga aggttataag cgtaag                      46


<210> 34
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 34
acacaagtga gaatcttgtg gacctaactc acagtcaaat gatgt                       45


<210> 35
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 35
cgaagtggaa aagggtttca cg                                                22


<210> 36
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 36
acttctagtg gtgttcgtca ggcttgt                                           27


<210> 37
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 37
ctaactttgg tgccgaatta agcca                                             25


<210> 38
<211> 43
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 38
agggaaatca gacgatgaca atgctatctc catttttatt taa                         43


<210> 39
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 39
tttgaccacc ttaaacatga atgaagtaac attttacatt tgga                        44


<210> 40
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 40
tctaaaagta tgtcaatgat aa                                                22


<210> 41
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 41
aagcatgctt gggatgcgat tctc                                              24


<210> 42
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 42
tgcaaccatt aaaactggcg                                                   20


<210> 43
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 43
tggagcgttt tctcctgaac                                                   20


<210> 44
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 44
tacgggcttc ccttcgcgat agtggattac ggttccgcag a                           41


<210> 45
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 45
gccgaagcct atggcgtgaa gtggctggca tccatgtt                               38


<210> 46
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 46
cccaggcata atctttttgt tcgt                                              24


<210> 47
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 47
atccagcgtt attgatatgg cc                                                22


<210> 48
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 48
agttcaagga cgcacatgc                                                    19


<210> 49
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 49
aacgctgcgr tacacaag                                                     18


<210> 50
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 50
gcctcyagag ctccagcaag aggcaaactg tcgtggttc                              39


<210> 51
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 51
gtgcaaaggg aaggctgtcc tcgagtatga cacgcccttc aa                          42


<210> 52
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 52
ctgctccttc ttgactccct a                                                 21


<210> 53
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 53
tggccacttg aaatgtcgc                                                    19


<210> 54
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 54
gaaggagccg ttcacacg                                                     18


<210> 55
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 55
cctgcatact gcacctcc                                                     18


<210> 56
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 56
ggcggcattt caaatggcca gctcgctgga gctctagagg                             40


<210> 57
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 57
tattccttgt gcactgcggc atgactgttc catgcagtgt t                           41


<210> 58
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 58
cctttgcacc atccatctca g                                                 21


<210> 59
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 59
ttcacattca ccaaggtccc a                                                 21


<210> 60
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 60
caaattattt tcctacacct agtgg                                             25


<210> 61
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 61
gtcataacgt ctgcagttaa gg                                                22


<210> 62
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 62
gtggccctgt gctcgttgtc tatggttacc tctgatgcc                              39


<210> 63
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 63
cacgcagtac aaatatgtca ccccatgtcg taggtactcc                             40


<210> 64
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 64
gctgccatat ctacttcaga aactaca                                           27


<210> 65
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 65
tgtattctcc ctctccaagt g                                                 21


<210> 66
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 66
gaatatagga cataacatct gcag                                              24


<210> 67
<211> 43
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 67
gccagcaaac accattgtta ctctattgtt acctctgact ccc                         43


<210> 68
<211> 46
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 68
accactcgca gtaccaattt aaccctcaac atgtctgcta tactgc                      46


<210> 69
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 69
accctgtgcc ttatgtaacc                                                   20


<210> 70
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 70
cctataggtg aacattggg                                                    19


<210> 71
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 71
ggatatttgc aaatggaact g                                                 21


<210> 72
<211> 43
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 72
gtttagtaac tccaaaggag gacaaaggca caccttgtaa tgc                         43


<210> 73
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 73
gggacatggt agacacagga catatatcta ggggaacatc ac                          42


<210> 74
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 74
cattctcctg cttttacctg gt                                                22


<210> 75
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 75
gaaacacaac gtttggtttg ggc                                               23


<210> 76
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 76
gtgctcacca atagcaggta c                                                 21


<210> 77
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 77
taaaatggat ggccacttag gccggtatgg aaattggtcg tgggc                       45


<210> 78
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 78
ggatgataca gaaagtgctc aaaatacaca gctgtgtttg c                           41


<210> 79
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 79
caatacctaa aggctgcc                                                     18


<210> 80
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 80
gccactgtac aaagcagtgc                                                   20


<210> 81
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 81
tgaatgtatg tcataacatc agctg                                             25


<210> 82
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 82
attattgtgg ccctgcgcac gttctatggt aacctcagaa tccc                        44


<210> 83
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 83
accactcgta gcactaacat gactcgccat gacgaaggta ttcct                       45


<210> 84
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 84
gctgaggtta aaaaggaaag caca                                              24


<210> 85
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 85
gacgtgagca gatgtttgt                                                    19


<210> 86
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 86
ccattgttat gaccttgtgc                                                   20


<210> 87
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 87
ggataactgc agtattaccg gacctagggc tggaaaactt gg                          42


<210> 88
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 88
tccaactcct agtggctcta tagcgctgta gccaataagg c                           41


<210> 89
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 89
cctcagaatc acaattattt aataagcc                                          28


<210> 90
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 90
cgaggttgat ggtgttcaaa ta                                                22


<210> 91
<211> 47
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      KRAS oligonucleotide 

<400> 91
ataaacttgt ggtagttgga gctggtggcg taggcaagag tgccttg                     47


<210> 92
<211> 47
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 92
ataaacttgt ggtagttgga gctgatggcg taggcaagag tgccttg                     47


<210> 93
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 93
tctacgccac cagctccaa                                                    19


<210> 94
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 94
cgucgucguu cuggggagcc                                                   20


<210> 95
<211> 45
<212> DNA
<213> Zika virus

<400> 95
aggcaaactg tcgtggttct agggagtcaa gaaggagcag ttcac                       45


<210> 96
<211> 45
<212> DNA
<213> Zika virus

<400> 96
aggcaaaccg tcgtcgttct ggggagccag gaaggagccg ttcac                       45


<210> 97
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 97
tctccttcct ggctcccca                                                    19


<210> 98
<211> 45
<212> DNA
<213> Zika virus

<400> 98
caaactgtcg tggttctagg gagtcaagaa ggagcagttc acacg                       45


<210> 99
<211> 45
<212> DNA
<213> Zika virus

<400> 99
caaactgtcg tggttctggg gagccaggaa ggagcagttc acacg                       45


