                         SEQUENCE LISTING

<110>  The Regents of the University of California
 
<120>  METHANOL DEHYDROGENASES

<130>  00011-086WO1

<140>  Not yet assigned
<141>  2017-01-12

<150>  US 62/277,849
<151>  2016-01-12

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  1173
<212>  DNA
<213>  Cupriavidus necator


<220>
<221>  CDS
<222>  (1)..(1173)

<400>  1
atg acc cac ctg aac atc gct aat cgc gtc gac agc ttc ttc att ccc         48
Met Thr His Leu Asn Ile Ala Asn Arg Val Asp Ser Phe Phe Ile Pro           
1               5                   10                  15                

tgc gtg acc ctc ttc ggt ccg ggc tgc gcg cgc gaa acg ggc gct cgc         96
Cys Val Thr Leu Phe Gly Pro Gly Cys Ala Arg Glu Thr Gly Ala Arg           
            20                  25                  30                    

gcc aga tca ctc ggg gcc agg aag gct ctc atc gtc acg gat gca ggc        144
Ala Arg Ser Leu Gly Ala Arg Lys Ala Leu Ile Val Thr Asp Ala Gly           
        35                  40                  45                        

ttg cac aag atg ggg ctc tcc gaa gtc gtc gcg ggg cac att cgc gaa        192
Leu His Lys Met Gly Leu Ser Glu Val Val Ala Gly His Ile Arg Glu           
    50                  55                  60                            

gcc ggg ctc cag gcc gtc atc ttt ccg ggt gcc gag ccc aat ccc acc        240
Ala Gly Leu Gln Ala Val Ile Phe Pro Gly Ala Glu Pro Asn Pro Thr           
65                  70                  75                  80            

gac gtt aac gtt cac gac ggc gtc aag ttg ttc gag cgg gaa gaa tgc        288
Asp Val Asn Val His Asp Gly Val Lys Leu Phe Glu Arg Glu Glu Cys           
                85                  90                  95                

gac ttc atc gtt tcg ctc ggc ggc ggc tca tcg cac gac tgc gcg aaa        336
Asp Phe Ile Val Ser Leu Gly Gly Gly Ser Ser His Asp Cys Ala Lys           
            100                 105                 110                   

ggc atc ggc ctc gtt acc gcc gga ggc gga cat atc cgc gac tac gaa        384
Gly Ile Gly Leu Val Thr Ala Gly Gly Gly His Ile Arg Asp Tyr Glu           
        115                 120                 125                       

ggc atc gac aaa tca acg gtg cca atg acg ccg ctg att tcg atc aac        432
Gly Ile Asp Lys Ser Thr Val Pro Met Thr Pro Leu Ile Ser Ile Asn           
    130                 135                 140                           

acg acc gct ggc act gct gcg gaa atg aca cgc ttt tgc atc atc act        480
Thr Thr Ala Gly Thr Ala Ala Glu Met Thr Arg Phe Cys Ile Ile Thr           
145                 150                 155                 160           

aat tcg agc aat cat gtg aag atg gca atc gtc gac tgg cgt tgc acg        528
Asn Ser Ser Asn His Val Lys Met Ala Ile Val Asp Trp Arg Cys Thr           
                165                 170                 175               

cca tta atc gcc atc gac gat ccg agc ctg atg gtc gcg atg ccg ccc        576
Pro Leu Ile Ala Ile Asp Asp Pro Ser Leu Met Val Ala Met Pro Pro           
            180                 185                 190                   

gcc ttg acg gcg gcg acc ggc atg gac gcg ttg act cac gcc atc gag        624
Ala Leu Thr Ala Ala Thr Gly Met Asp Ala Leu Thr His Ala Ile Glu           
        195                 200                 205                       

gca tac gtt tcc acc gcc gcc acg cca att acc gat gcc tgt gcg gag        672
Ala Tyr Val Ser Thr Ala Ala Thr Pro Ile Thr Asp Ala Cys Ala Glu           
    210                 215                 220                           

aag gcg atc gtg ctg atc gcc gaa tgg ctg ccc aaa gct gtc gcg aac        720
Lys Ala Ile Val Leu Ile Ala Glu Trp Leu Pro Lys Ala Val Ala Asn           
225                 230                 235                 240           

ggg gac tcg atg gaa gca cgc gcg gcc atg tgc tac gcc caa tac ctt        768
Gly Asp Ser Met Glu Ala Arg Ala Ala Met Cys Tyr Ala Gln Tyr Leu           
                245                 250                 255               

gcc ggc atg gcc ttc aac aac gca tca ctc ggt tac gtg cac gcg atg        816
Ala Gly Met Ala Phe Asn Asn Ala Ser Leu Gly Tyr Val His Ala Met           
            260                 265                 270                   

gcc cat caa ctc ggc ggc ttc tac aat ttg ccc cac ggc gtg tgc aac        864
Ala His Gln Leu Gly Gly Phe Tyr Asn Leu Pro His Gly Val Cys Asn           
        275                 280                 285                       

gcg atc ctg ctg ccg cac gtg tcg gaa ttc aac ctc att gcc gcg ccg        912
Ala Ile Leu Leu Pro His Val Ser Glu Phe Asn Leu Ile Ala Ala Pro           
    290                 295                 300                           

gag cgc tac gcg aga atc gcc gaa ctg cta ggc gag aac att ggg ggc        960
Glu Arg Tyr Ala Arg Ile Ala Glu Leu Leu Gly Glu Asn Ile Gly Gly           
305                 310                 315                 320           

ttg agc gcg cat gac gcc gcc aaa gct gcc gtc tcg gcg atc cgg acc       1008
Leu Ser Ala His Asp Ala Ala Lys Ala Ala Val Ser Ala Ile Arg Thr           
                325                 330                 335               

ctt tcc acg tcg att ggc att ccg gcg ggt ctg gcg ggc ctg ggc gtc       1056
Leu Ser Thr Ser Ile Gly Ile Pro Ala Gly Leu Ala Gly Leu Gly Val           
            340                 345                 350                   

aag gcg gac gac cat gaa gtg atg gca agc aat gcg caa aag gat gct       1104
Lys Ala Asp Asp His Glu Val Met Ala Ser Asn Ala Gln Lys Asp Ala           
        355                 360                 365                       

tgc atg ctg acg aat ccg cgc aag gcc acg ctg gcg caa gtc atg gca       1152
Cys Met Leu Thr Asn Pro Arg Lys Ala Thr Leu Ala Gln Val Met Ala           
    370                 375                 380                           

atc ttc gct gcg gcg atg taa                                           1173
Ile Phe Ala Ala Ala Met                                                   
385                 390                                                   


<210>  2
<211>  390
<212>  PRT
<213>  Cupriavidus necator

<400>  2

Met Thr His Leu Asn Ile Ala Asn Arg Val Asp Ser Phe Phe Ile Pro 
1               5                   10                  15      


Cys Val Thr Leu Phe Gly Pro Gly Cys Ala Arg Glu Thr Gly Ala Arg 
            20                  25                  30          


Ala Arg Ser Leu Gly Ala Arg Lys Ala Leu Ile Val Thr Asp Ala Gly 
        35                  40                  45              


Leu His Lys Met Gly Leu Ser Glu Val Val Ala Gly His Ile Arg Glu 
    50                  55                  60                  


Ala Gly Leu Gln Ala Val Ile Phe Pro Gly Ala Glu Pro Asn Pro Thr 
65                  70                  75                  80  


Asp Val Asn Val His Asp Gly Val Lys Leu Phe Glu Arg Glu Glu Cys 
                85                  90                  95      


Asp Phe Ile Val Ser Leu Gly Gly Gly Ser Ser His Asp Cys Ala Lys 
            100                 105                 110         


Gly Ile Gly Leu Val Thr Ala Gly Gly Gly His Ile Arg Asp Tyr Glu 
        115                 120                 125             


Gly Ile Asp Lys Ser Thr Val Pro Met Thr Pro Leu Ile Ser Ile Asn 
    130                 135                 140                 


Thr Thr Ala Gly Thr Ala Ala Glu Met Thr Arg Phe Cys Ile Ile Thr 
145                 150                 155                 160 


Asn Ser Ser Asn His Val Lys Met Ala Ile Val Asp Trp Arg Cys Thr 
                165                 170                 175     


Pro Leu Ile Ala Ile Asp Asp Pro Ser Leu Met Val Ala Met Pro Pro 
            180                 185                 190         


Ala Leu Thr Ala Ala Thr Gly Met Asp Ala Leu Thr His Ala Ile Glu 
        195                 200                 205             


Ala Tyr Val Ser Thr Ala Ala Thr Pro Ile Thr Asp Ala Cys Ala Glu 
    210                 215                 220                 


Lys Ala Ile Val Leu Ile Ala Glu Trp Leu Pro Lys Ala Val Ala Asn 
225                 230                 235                 240 


Gly Asp Ser Met Glu Ala Arg Ala Ala Met Cys Tyr Ala Gln Tyr Leu 
                245                 250                 255     


Ala Gly Met Ala Phe Asn Asn Ala Ser Leu Gly Tyr Val His Ala Met 
            260                 265                 270         


Ala His Gln Leu Gly Gly Phe Tyr Asn Leu Pro His Gly Val Cys Asn 
        275                 280                 285             


Ala Ile Leu Leu Pro His Val Ser Glu Phe Asn Leu Ile Ala Ala Pro 
    290                 295                 300                 


Glu Arg Tyr Ala Arg Ile Ala Glu Leu Leu Gly Glu Asn Ile Gly Gly 
305                 310                 315                 320 


Leu Ser Ala His Asp Ala Ala Lys Ala Ala Val Ser Ala Ile Arg Thr 
                325                 330                 335     


Leu Ser Thr Ser Ile Gly Ile Pro Ala Gly Leu Ala Gly Leu Gly Val 
            340                 345                 350         


Lys Ala Asp Asp His Glu Val Met Ala Ser Asn Ala Gln Lys Asp Ala 
        355                 360                 365             


Cys Met Leu Thr Asn Pro Arg Lys Ala Thr Leu Ala Gln Val Met Ala 
    370                 375                 380                 


Ile Phe Ala Ala Ala Met 
385                 390 


<210>  3
<211>  363
<212>  PRT
<213>  Cupriavidus necator

<400>  3

Met Ile His Ala Tyr His Asn Pro Val Ser Ile His Cys Gly Ala Gly 
1               5                   10                  15      


Ser Leu Asp Gln Leu Pro Ala Leu Leu His Gly Arg Arg Val Val Val 
            20                  25                  30          


Val Thr Phe Pro Glu Ala Arg Ala Leu Gly Leu Val Ala Arg Leu Glu 
        35                  40                  45              


Ala Leu Leu Gly Ala Asp Leu Val Gly Met Ile Glu Asp Val Arg Pro 
    50                  55                  60                  


Asn Pro Asp Val Ala Gln Leu Ser Gly Leu Tyr Asp Arg Phe Trp Glu 
65                  70                  75                  80  


His Ala Asp Ala Cys Asp Ala Ile Val Ala Val Gly Gly Gly Ser Ala 
                85                  90                  95      


Ile Asp Thr Ala Lys Ala Leu Met Val Gly Thr Arg Thr Gly Arg Phe 
            100                 105                 110         


Asp Ser Leu Ile Ser Leu Leu Ala Ala Gly Lys Pro Phe Thr Pro Ala 
        115                 120                 125             


Gln Val Lys Pro Leu Ile Ala Val Pro Thr Thr Ala Gly Thr Gly Ser 
    130                 135                 140                 


Glu Val Thr Pro Trp Ala Thr Ile Trp Asp Ala Ala Lys Gln Lys Lys 
145                 150                 155                 160 


Tyr Ser Leu His Leu Glu Ala Thr Trp Pro Glu Ala Ala Ile Val Asp 
                165                 170                 175     


Ala Glu Leu Met Met Ser Val Pro Arg Gly Thr Thr Ile Ser Thr Gly 
            180                 185                 190         


Leu Asp Ala Leu Ser His Ala Leu Glu Ser Ile Trp Asn Val Asn Ala 
        195                 200                 205             


Asn Pro Ile Ser Asp Thr Phe Ala Ile Ser Ala Val Glu Asp Ile Phe 
    210                 215                 220                 


Asp Thr Leu Pro Glu Leu Val Arg Asn Leu Asp Asp Ile Ala Leu Arg 
225                 230                 235                 240 


Glu Arg Met Ala Leu Ala Ala Leu Lys Ala Gly Leu Ala Phe Ser Asn 
                245                 250                 255     


Thr Lys Thr Ala Leu Ala His Ser Ile Ser Tyr Glu Met Thr Leu Arg 
            260                 265                 270         


His Gly Leu Pro His Gly Ile Ala Cys Ser Phe Thr Leu Pro Met Val 
        275                 280                 285             


Leu Glu Lys Ala Trp Ala Arg Arg Pro Asp Arg Asp Arg Thr Leu Gln 
    290                 295                 300                 


Arg Leu Phe Gly Pro Val Leu Asp His Gly Gln Gln Arg Leu Arg Ala 
305                 310                 315                 320 


Phe Leu Gln Gly Leu Gly Val Lys Thr Glu Phe Ala Asp Tyr Gly Val 
                325                 330                 335     


Thr Asp Asp Glu Ala Gly Ala Met Ile Thr Arg Ala Leu Asp Gly Ala 
            340                 345                 350         


Arg Gly Lys Asn Phe Ile Gly Ala Val Ser Ala 
        355                 360             


<210>  4
<211>  383
<212>  PRT
<213>  Zymomonas mobilis

<400>  4

Met Ala Ser Ser Thr Phe Tyr Ile Pro Phe Val Asn Glu Met Gly Glu 
1               5                   10                  15      


Gly Ser Leu Glu Lys Ala Ile Lys Asp Leu Asn Gly Ser Gly Phe Lys 
            20                  25                  30          


Asn Ala Leu Ile Val Ser Asp Ala Phe Met Asn Lys Ser Gly Val Val 
        35                  40                  45              


Lys Gln Val Ala Asp Leu Leu Lys Thr Gln Gly Ile Asn Ser Ala Val 
    50                  55                  60                  


Tyr Asp Gly Val Met Pro Asn Pro Thr Val Thr Ala Val Leu Glu Gly 
65                  70                  75                  80  


Leu Lys Ile Leu Lys Asp Asn Asn Ser Asp Phe Val Ile Ser Leu Gly 
                85                  90                  95      


Gly Gly Ser Pro His Asp Cys Ala Lys Ala Ile Ala Leu Val Ala Thr 
            100                 105                 110         


Asn Gly Gly Glu Val Lys Asp Tyr Glu Gly Ile Asp Lys Ser Lys Lys 
        115                 120                 125             


Pro Ala Leu Pro Leu Met Ser Ile Asn Thr Thr Ala Gly Thr Ala Ser 
    130                 135                 140                 


Glu Met Thr Arg Phe Cys Ile Ile Thr Asp Glu Val Arg His Val Lys 
145                 150                 155                 160 


Met Ala Ile Val Asp Arg His Val Thr Pro Met Val Ser Val Asn Asp 
                165                 170                 175     


Pro Leu Leu Met Val Gly Met Pro Lys Gly Leu Thr Ala Ala Thr Gly 
            180                 185                 190         


Met Asp Ala Leu Thr His Ala Phe Glu Ala Tyr Ser Ser Thr Ala Ala 
        195                 200                 205             


Thr Pro Ile Thr Asp Ala Cys Ala Leu Lys Ala Ala Ser Met Ile Ala 
    210                 215                 220                 


Lys Asn Leu Lys Thr Ala Cys Asp Asn Gly Lys Asp Met Pro Ala Arg 
225                 230                 235                 240 


Glu Ala Met Ala Tyr Ala Gln Phe Leu Ala Gly Met Ala Phe Asn Asn 
                245                 250                 255     


Ala Ser Leu Gly Tyr Val His Ala Met Ala His Gln Leu Gly Gly Tyr 
            260                 265                 270         


Tyr Asn Leu Pro His Gly Val Cys Asn Ala Val Leu Leu Pro His Val 
        275                 280                 285             


Leu Ala Tyr Asn Ala Ser Val Val Ala Gly Arg Leu Lys Asp Val Gly 
    290                 295                 300                 


Val Ala Met Gly Leu Asp Ile Ala Asn Leu Gly Asp Lys Glu Gly Ala 
305                 310                 315                 320 


Glu Ala Thr Ile Gln Ala Val Arg Asp Leu Ala Ala Ser Ile Gly Ile 
                325                 330                 335     


Pro Ala Asn Leu Thr Glu Leu Gly Ala Lys Lys Glu Asp Val Pro Leu 
            340                 345                 350         


Leu Ala Asp His Ala Leu Lys Asp Ala Cys Ala Leu Thr Asn Pro Arg 
        355                 360                 365             


Gln Gly Asp Gln Lys Glu Val Glu Glu Leu Phe Leu Ser Ala Phe 
    370                 375                 380             


<210>  5
<211>  387
<212>  PRT
<213>  Klebsiella pneumoniae

<400>  5

Met Ser Tyr Arg Met Phe Asp Tyr Leu Val Pro Asn Val Asn Phe Phe 
1               5                   10                  15      


Gly Pro Asn Ala Ile Ser Val Val Gly Glu Arg Cys Gln Leu Leu Gly 
            20                  25                  30          


Gly Lys Lys Ala Leu Leu Val Thr Asp Lys Gly Leu Arg Ala Ile Lys 
        35                  40                  45              


Asp Gly Ala Val Asp Lys Thr Leu His Tyr Leu Arg Glu Ala Gly Ile 
    50                  55                  60                  


Glu Val Ala Ile Phe Asp Gly Val Glu Pro Asn Pro Lys Asp Thr Asn 
65                  70                  75                  80  


Val Arg Asp Gly Leu Ala Val Phe Arg Arg Glu Gln Cys Asp Ile Ile 
                85                  90                  95      


Val Thr Val Gly Gly Gly Ser Pro His Asp Cys Gly Lys Gly Ile Gly 
            100                 105                 110         


Ile Ala Ala Thr His Glu Gly Asp Leu Tyr Gln Tyr Ala Gly Ile Glu 
        115                 120                 125             


Thr Leu Thr Asn Pro Leu Pro Pro Ile Val Ala Val Asn Thr Thr Ala 
    130                 135                 140                 


Gly Thr Ala Ser Glu Val Thr Arg His Cys Val Leu Thr Asn Thr Glu 
145                 150                 155                 160 


Thr Lys Val Lys Phe Val Ile Val Ser Trp Arg Asn Leu Pro Ser Val 
                165                 170                 175     


Ser Ile Asn Asp Pro Leu Leu Met Ile Gly Lys Pro Ala Ala Leu Thr 
            180                 185                 190         


Ala Ala Thr Gly Met Asp Ala Leu Thr His Ala Val Glu Ala Tyr Ile 
        195                 200                 205             


Ser Lys Asp Ala Asn Pro Val Thr Asp Ala Ala Ala Met Gln Ala Ile 
    210                 215                 220                 


Arg Leu Ile Ala Arg Asn Leu Arg Gln Ala Val Ala Leu Gly Ser Asn 
225                 230                 235                 240 


Leu Gln Ala Arg Glu Tyr Met Ala Tyr Ala Ser Leu Leu Ala Gly Met 
                245                 250                 255     


Ala Phe Asn Asn Ala Asn Leu Gly Tyr Val His Ala Met Ala His Gln 
            260                 265                 270         


Leu Gly Gly Leu Tyr Asp Met Pro His Gly Val Ala Asn Ala Val Leu 
        275                 280                 285             


Leu Pro His Val Ala Arg Tyr Asn Leu Ile Ala Asn Pro Glu Lys Phe 
    290                 295                 300                 


Ala Asp Ile Ala Glu Leu Met Gly Glu Asn Ile Thr Gly Leu Ser Thr 
305                 310                 315                 320 


Leu Asp Ala Ala Glu Lys Ala Ile Ala Ala Ile Thr Arg Leu Ser Met 
                325                 330                 335     


Asp Ile Gly Ile Pro Gln His Leu Arg Asp Leu Gly Val Lys Glu Thr 
            340                 345                 350         


Asp Phe Pro Tyr Met Ala Glu Met Ala Leu Lys Asp Gly Asn Ala Phe 
        355                 360                 365             


Ser Asn Pro Arg Lys Gly Asn Glu Gln Glu Ile Ala Ala Ile Phe Arg 
    370                 375                 380                 


Gln Ala Phe 
385         


<210>  6
<211>  382
<212>  PRT
<213>  Bacillus methanolicus

<400>  6

Met Thr Thr Asn Phe Phe Ile Pro Pro Ala Ser Val Ile Gly Arg Gly 
1               5                   10                  15      


Ala Val Lys Glu Val Gly Thr Arg Leu Lys Gln Ile Gly Ala Lys Lys 
            20                  25                  30          


Ala Leu Ile Val Thr Asp Ala Phe Leu His Ser Thr Gly Leu Ser Glu 
        35                  40                  45              


Glu Val Ala Lys Asn Ile Arg Glu Ala Gly Val Asp Val Ala Ile Phe 
    50                  55                  60                  


Pro Lys Ala Gln Pro Asp Pro Ala Asp Thr Gln Val His Glu Gly Val 
65                  70                  75                  80  


Asp Val Phe Lys Gln Glu Asn Cys Asp Ser Leu Val Ser Ile Gly Gly 
                85                  90                  95      


Gly Ser Ser His Asp Thr Ala Lys Ala Ile Gly Leu Val Ala Ala Asn 
            100                 105                 110         


Gly Gly Arg Ile Asn Asp Tyr Gln Gly Val Asn Ser Val Glu Lys Pro 
        115                 120                 125             


Val Val Pro Val Val Ala Ile Thr Thr Thr Ala Gly Thr Gly Ser Glu 
    130                 135                 140                 


Thr Thr Ser Leu Ala Val Ile Thr Asp Ser Ala Arg Lys Val Lys Met 
145                 150                 155                 160 


Pro Val Ile Asp Glu Lys Ile Thr Pro Thr Val Ala Ile Val Asp Pro 
                165                 170                 175     


Glu Leu Met Val Lys Lys Pro Ala Gly Leu Thr Ile Ala Thr Gly Met 
            180                 185                 190         


Asp Ala Leu Ser His Ala Ile Glu Ala Tyr Val Ala Lys Gly Ala Thr 
        195                 200                 205             


Pro Val Thr Asp Ala Phe Ala Ile Gln Ala Met Lys Leu Ile Asn Glu 
    210                 215                 220                 


Tyr Leu Pro Lys Ala Val Ala Asn Gly Glu Asp Ile Glu Ala Arg Glu 
225                 230                 235                 240 


Lys Met Ala Tyr Ala Gln Tyr Met Ala Gly Val Ala Phe Asn Asn Gly 
                245                 250                 255     


Gly Leu Gly Leu Val His Ser Ile Ser His Gln Val Gly Gly Val Tyr 
            260                 265                 270         


Lys Leu Gln His Gly Ile Cys Asn Ser Val Asn Met Pro His Val Cys 
        275                 280                 285             


Ala Phe Asn Leu Ile Ala Lys Thr Glu Arg Phe Ala His Ile Ala Glu 
    290                 295                 300                 


Leu Leu Gly Glu Asn Val Ala Gly Leu Ser Thr Ala Ala Ala Ala Glu 
305                 310                 315                 320 


Arg Ala Ile Val Ala Leu Glu Arg Ile Asn Lys Ser Phe Gly Ile Pro 
                325                 330                 335     


Ser Gly Tyr Ala Glu Met Gly Val Lys Glu Glu Asp Ile Glu Leu Leu 
            340                 345                 350         


Ala Lys Asn Ala Tyr Glu Asp Val Cys Thr Gln Ser Asn Pro Arg Val 
        355                 360                 365             


Pro Thr Val Gln Asp Ile Ala Gln Ile Ile Lys Asn Ala Met 
    370                 375                 380         


<210>  7
<211>  401
<212>  PRT
<213>  Desulfitobacterium hafniense

<400>  7

Met Lys Asn Ile Asn Lys Gln Gln Glu Glu Ile Ile Met Phe Asn Met 
1               5                   10                  15      


Tyr Val Pro Thr Arg Phe Val Phe Gly Ser Gly Arg Leu Arg Glu Leu 
            20                  25                  30          


His Gln Gln Lys Met Pro Gly Lys Lys Ala Leu Ile Thr Ile Ser Asn 
        35                  40                  45              


Gly Lys Ser Val Arg Glu Asn Gly Ala Leu Asp Arg Thr Gln Glu Gln 
    50                  55                  60                  


Leu Arg Leu Ala Gly Val Ala Met Val Val Phe Asp Lys Ile Gly Ala 
65                  70                  75                  80  


Asn Pro Thr Lys Thr Ala Val Met Glu Gly Ala Lys Ser Ala Arg Asp 
                85                  90                  95      


His Gly Cys Asp Phe Ile Val Ala Leu Gly Gly Gly Ser Val Met Asp 
            100                 105                 110         


Ala Ala Lys Ala Ile Ala Met Met Ala Thr Asn Asp Gly Asp Leu Trp 
        115                 120                 125             


Asp Tyr Val Gly Gly Lys Thr Gly Lys Gly Gln Ala Leu Arg His Glu 
    130                 135                 140                 


Pro Leu Pro Leu Val Cys Ile Thr Thr Thr Ala Gly Thr Gly Ser Glu 
145                 150                 155                 160 


Ala Asp Gln Trp Gly Val Val Thr Asn Glu Glu Thr Asn Glu Lys Ile 
                165                 170                 175     


Gly Val Gly Gly Tyr Asp Ala Ser Phe Pro Val Val Ser Ile Ile Asp 
            180                 185                 190         


Pro Glu Leu Met Val Ser Val Pro Pro Lys Phe Thr Ala Tyr Gln Gly 
        195                 200                 205             


Phe Asp Ala Leu Phe His Ala Thr Glu Gly Tyr Ile Ser Lys Phe Ala 
    210                 215                 220                 


Ser Pro Met Ser Asp Met Tyr Ala Leu Thr Ala Ile Glu Asn Val Gly 
225                 230                 235                 240 


Asn Tyr Leu Val Arg Ala Cys Arg Asp Gly Arg Asp Met Glu Ala Arg 
                245                 250                 255     


Glu Gly Met Ala Phe Ala Asn Thr Leu Ser Gly Val Val Met Thr Val 
            260                 265                 270         


Ser Val Thr Thr Ala Glu His Ser Leu Glu His Ala Met Ser Ala Tyr 
        275                 280                 285             


His Pro Glu Leu Pro His Gly Ala Gly Leu Ile Met Ile Ser Arg Ala 
    290                 295                 300                 


Phe Tyr Glu Phe Phe Ile Glu Lys His Ala Cys Asp Gln Arg Phe Val 
305                 310                 315                 320 


Arg Met Ala Gln Ala Leu Gly Met Lys Asp Ala Ala Arg Pro Glu Asp 
                325                 330                 335     


Phe Ile Thr Met Leu Val Lys Leu Gln Glu Asn Cys Gly Val Ala Asp 
            340                 345                 350         


Leu Lys Met Ser Asp Tyr Gly Met Thr Pro Asp Glu Phe Asp Lys Leu 
        355                 360                 365             


Ala Ala Asn Ala Arg Glu Thr Met Gly Gly Leu Phe Ala Ala Asn Pro 
    370                 375                 380                 


Tyr Ala Met Thr His Glu Asp Cys Val Glu Val Tyr Arg Lys Ser Tyr 
385                 390                 395                 400 


Arg 
    


<210>  8
<211>  393
<212>  PRT
<213>  Lysinibacillus sphaericus

<400>  8

Met His Lys Leu Thr Phe Thr Pro Val Ser Tyr Thr Gly Trp Gly Cys 
1               5                   10                  15      


Leu Asn Gln Leu Leu Pro Glu Val Glu Arg Leu Lys Ala Thr Asn Ile 
            20                  25                  30          


Leu Ile Val Thr Asp Pro Phe Leu Lys Glu Leu Gly Leu Thr Asp Lys 
        35                  40                  45              


Val Gln Gln Pro Leu Leu Ser Lys Gly Tyr Ala Thr Thr Ile Tyr Thr 
    50                  55                  60                  


Asp Ile Ala Pro Glu Pro Pro Leu Ala Ile Gly Glu Lys Leu Val Asp 
65                  70                  75                  80  


Phe Thr Arg Lys His Gln Phe Asp Leu Val Ile Gly Leu Gly Gly Gly 
                85                  90                  95      


Ser Ala Leu Asp Leu Ala Lys Leu Ala Ala Val Leu Ala Val His Asp 
            100                 105                 110         


Gly Lys Val Ala Asp Tyr Leu Asn Leu Thr Gly Thr Glu Ala Leu Glu 
        115                 120                 125             


Gln Lys Gly Leu Pro Lys Ile Leu Ile Pro Asn Thr Ser Gly Thr Gly 
    130                 135                 140                 


Ser Glu Val Thr Asn Ile Ser Val Leu Ser Leu Asp Thr Thr Lys Asp 
145                 150                 155                 160 


Val Val Thr His Asp Tyr Leu Leu Ala Asp Ser Ala Ile Val Asp Pro 
                165                 170                 175     


Glu Leu Thr Ile Ser Leu Pro Pro Lys Val Thr Ala Ala Thr Gly Val 
            180                 185                 190         


Asp Ala Leu Thr His Ala Ile Glu Ala Tyr Val Ser Val Asn Ala Asn 
        195                 200                 205             


Glu Val Thr Asp Ser Leu Ala Leu Gln Ala Ile Arg Leu Ile Ser Gly 
    210                 215                 220                 


Ser Ile Arg Thr Ala Val Leu Glu Gly Glu Asn Lys Gln Ala Arg Ser 
225                 230                 235                 240 


Asp Met Ser Tyr Gly Ser Tyr Leu Ala Gly Leu Ala Phe Phe Asn Ala 
                245                 250                 255     


Gly Val Ala Gly Val His Ala Leu Ala Tyr Pro Leu Gly Gly Gln Phe 
            260                 265                 270         


His Ile Ala His Gly Asp Ser Asn Ala Val Leu Leu Pro Tyr Val Met 
        275                 280                 285             


Gly Tyr Ile Arg Gln Ser Cys Glu Lys Arg Met Lys Asp Ile Leu Asp 
    290                 295                 300                 


Ala Met Gly Leu Ser Ser Ala Tyr Leu Ser Gln Glu Glu Ala Ser Tyr 
305                 310                 315                 320 


Lys Cys Val Asp Ala Leu Gln Gln Leu Val Gln Asp Val Asn Ile Pro 
                325                 330                 335     


Ser Thr Leu Lys Gly Phe Asp Ile Pro Glu Glu Ala Leu Glu Gln Leu 
            340                 345                 350         


Thr Asp Asp Ala Thr Lys Gln Thr Arg Ile Leu Ala Arg Ser Pro Met 
        355                 360                 365             


Pro Leu Glu Arg Glu Asp Ile Tyr Ala Ile Tyr Arg Ala Ala Phe Asp 
    370                 375                 380                 


Gly Glu Val Arg Glu Pro Asp Ala Ser 
385                 390             


<210>  9
<211>  390
<212>  PRT
<213>  Lysinibacillus fusiformis

<400>  9

Met His Lys Leu Thr Phe Thr Pro Ala Ser Tyr Thr Gly Trp Gly Cys 
1               5                   10                  15      


Leu Ser Gln Leu Leu Leu Glu Val Lys Lys Phe Asn Ala His Asn Ile 
            20                  25                  30          


Leu Ile Val Thr Asp Pro Leu Leu Lys Glu Ile Gly Leu Thr Asp Asn 
        35                  40                  45              


Val Glu Lys Pro Leu Ile Glu Lys Gly Tyr Thr Thr Thr Ile Tyr Thr 
    50                  55                  60                  


Asp Ile Ala Pro Glu Pro Pro Leu Ala Ile Gly Glu Lys Leu Val Asp 
65                  70                  75                  80  


Phe Thr Arg Lys His Pro Phe Asp Leu Val Ile Gly Val Gly Gly Gly 
                85                  90                  95      


Ser Ala Leu Asp Leu Ala Lys Leu Ala Ala Val Leu Ala Lys His Asp 
            100                 105                 110         


Gly Lys Val Ala Asp Tyr Leu Asn Leu Thr Gly Thr Lys Thr Leu Asp 
        115                 120                 125             


Asn Lys Gly Leu Pro Lys Ile Leu Ile Pro Thr Thr Ser Gly Thr Gly 
    130                 135                 140                 


Ser Glu Val Thr Asn Ile Ser Val Leu Ser Leu Asp Thr Thr Lys Asp 
145                 150                 155                 160 


Val Val Thr His Asp Tyr Leu Leu Ala Asp Val Ala Ile Val Asp Pro 
                165                 170                 175     


Ala Leu Thr Ile Ser Leu Pro Ser Lys Val Thr Ala Ala Thr Gly Val 
            180                 185                 190         


Asp Ala Leu Thr His Ala Ile Glu Ala Tyr Val Ser Val Asn Ala Asn 
        195                 200                 205             


Ala Val Thr Asp Ala Leu Ala Leu Gln Ala Ile Arg Leu Ile Ser Gln 
    210                 215                 220                 


Ser Ile Arg Thr Ala Val His Asn Gly Gln Asp Lys Gln Ala Arg Thr 
225                 230                 235                 240 


Asp Met Ser Tyr Gly Ser Tyr Leu Ala Gly Leu Ala Phe Phe Asn Ala 
                245                 250                 255     


Gly Val Ala Gly Val His Ala Leu Ala Tyr Pro Leu Gly Gly Gln Phe 
            260                 265                 270         


His Ile Ala His Gly Asp Ser Asn Ala Val Leu Leu Pro Tyr Val Met 
        275                 280                 285             


Gly Tyr Ile Arg Gln Ser Cys Glu Lys Arg Met Lys Asp Ile Leu Asp 
    290                 295                 300                 


Ala Met Gly Ile Ser Ser Ser Tyr Leu Ser Gln Glu Glu Ala Ser Tyr 
305                 310                 315                 320 


Lys Cys Val Asp Thr Leu Gln Gln Leu Val Gln Asp Val Lys Ile Pro 
                325                 330                 335     


Ser Thr Leu Gln Gly Phe Asn Ile Pro Ala Ser Ala Leu Glu Gln Leu 
            340                 345                 350         


Thr Glu Asp Ala Thr Lys Gln Thr Arg Ile Leu Ala Arg Ser Pro Met 
        355                 360                 365             


Pro Leu Glu Arg Glu Asp Ile Tyr Gln Ile Tyr Gln Ala Ala Phe Asp 
    370                 375                 380                 


Gly Val Val Arg Glu Pro 
385                 390 


<210>  10
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer T989

<400>  10
tctagaggca tcaaataaaa cgaaa                                             25


<210>  11
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer T990

<400>  11
tccctgaaaa tacaggtttt cggat                                             25


<210>  12
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer T1151

<400>  12
atccgaaaac ctgtattttc agggaatgac cactgctgca cccca                       45


<210>  13
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer T1152

<400>  13
tttcgtttta tttgatgcct ctagattaga aacgaatcgc cacac                       45


<210>  14
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer T1478

<400>  14
atccgaaaac ctgtattttc agggaatgct taagccagac aacct                       45


<210>  15
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer T1479

<400>  15
tttcgtttta tttgatgcct ctagattatg cccactcatt tttta                       45


<210>  16
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IWB094

<400>  16
tctagaggca tcaaataaaa cgaaaggc                                          28


<210>  17
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IWB141

<400>  17
tccctgaaaa tacaggtttt cggatccgtg atggtgatgg tgatgcgatc c                51


<210>  18
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IWB445

<400>  18
tccgaaaacc tgtattttca gggaatggga aaattatttg aggaaaaaac aattaaaac        59


<210>  19
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IWB446

<400>  19
gcctttcgtt ttatttgatg cctctagatc atttatgttt gagagcctct tgaagctgc        59


<210>  20
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer CT64

<400>  20
ggatccgaaa acctgtattt tcagggaatg acccacctga acatcgcta                   49


<210>  21
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer CT65

<400>  21
gagcctttcg ttttatttga tgcctctaga ttacatcgcc gcagcgaaga ttgcc            55


<210>  22
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer CT74

<400>  22
cggatccgaa aacctgtatt ttcagggaat gatccatgcc taccacaacc                  50


<210>  23
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer CT75

<400>  23
cctttcgttt tatttgatgc ctctagacta ggcagacacg gcgccgataa a                51


<210>  24
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer CT291


<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  n is a, c, g, or t

<400>  24
cgagcaatca tgtgaagatg nnkatcgtcg actggcgttg cac                         43


<210>  25
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer CT292


<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n is a, c, g, or t

<400>  25
gtgcaacgcc agtcgacgat mnncatcttc acatgattgc tcg                         43


<210>  26
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MS14

<400>  26
aaaacctgta ttttcaggga gaagtttatc aaaagcactc acatg                       45


<210>  27
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MS15

<400>  27
ttttatttga tgcctctaga tcaacgatca ggcaagactc tttca                       45


<210>  28
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MS16

<400>  28
aaaacctgta ttttcaggga cgtcctgctt tcgatcccga atccc                       45


<210>  29
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MS17

<400>  29
ttttatttga tgcctctaga tcaggccgcc agcaggtggt aaaga                       45


<210>  30
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MS33

<400>  30
aaaacctgta ttttcaggga atgaaattct gctcgaactg tggtc                       45


<210>  31
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MS34

<400>  31
ttttatttga tgcctctaga tcagggcgtg accgtggccc ggctg                       45


<210>  32
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MS35

<400>  32
aaaacctgta ttttcaggga atgtcctaca agatcccgga atccg                       45


<210>  33
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MS36

<400>  33
ttttatttga tgcctctaga tcatggctgc gctccgtaca ccgcc                       45


<210>  34
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MS37

<400>  34
aaaacctgta ttttcaggga atgaccgaca agatccaacg cggca                       45


<210>  35
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer MS38

<400>  35
ttttatttga tgcctctaga ctatatggcg taatgcggca gcggc                       45


<210>  36
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NAD-binding motif


<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  X can be any amino acid

<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  X can be any amino acid

<400>  36

Gly Gly Gly Ser Xaa Xaa Asp Xaa Xaa Lys 
1               5                   10  


