
                                SEQUENCE LISTING

<110> Seattle Children's Hospital dba Seattle Children's Research Institute
      Jensen, Michael C.
      Johnson, Adam

<120> TUMOR-SPECIFIC IFNA SECRETION BY CAR
  T-CELLS TO REPROGRAM THE SOLID TUMOR MICROENVIRONMENT
  

<130> SCRI.109PR

<150> 62/278648           
<151> 2016-01-14  

<160> 17

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 495
<212> DNA
<213> Artificial Sequence

<220> 
<223> IFNa2a

<400> 1
tgcgacctgc ctcagacaca cagcctgggc agcagacgga ccctgatgct gctggcccag 60
atgcggaaga tcagcctgtt cagctgcctg aaggaccggc acgacttcgg cttccctcag 120
gaagagttcg gcaaccagtt tcagaaggcc gagacaatcc ccgtgctgca cgagatgatc 180
cagcagatct tcaacctgtt ctccaccaag gacagcagcg ccgcctggga cgagacactg 240
ctggacaagt tctacaccga gctgtaccag cagctgaatg acctggaagc ctgcgtgatc 300
cagggcgtgg gcgtgacaga gacacccctg atgaaggaag atagcatcct ggccgtgcgc 360
aagtacttcc agcggatcac cctgtacctg aaagagaaga agtacagccc ctgcgcctgg 420
gaggtcgtgc gcgccgagat catgagaagc ttcagcctga gcaccaacct gcaggaaagc 480
ctgcggagca aagaa                                                  495

<210> 2
<211> 245
<212> DNA
<213> Artificial Sequence

<220> 
<223> EF1p domain

<400> 2
ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg 60
agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa 120
actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt 180
atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac 240
agctg                                                             245

<210> 3
<211> 510
<212> DNA
<213> Artificial Sequence

<220> 
<223> PD1ECD

<400> 3
atgcagatcc ctcaggcccc ttggcctgtc gtgtgggctg tgctgcagct gggatggcgg 60
cctggctggt ttctggacag ccccgacaga ccctggaacc cccctacatt ttcccctgcc 120
ctgctggtcg tgaccgaggg cgacaatgcc accttcacct gtagcttcag caacaccagc 180
gagagcttcg tgctgaactg gtacagaatg agccccagca accagaccga caagctggcc 240
gccttccccg aggatagatc tcagcccggc caggactgcc ggttcagagt gacccagctg 300
cccaacggcc gggacttcca catgtctgtc gtgcgggcca gacggaacga cagcggcaca 360
tatctgtgcg gcgccatcag cctggccccc aaggcccaga tcaaagagag cctgagagcc 420
gagctgagag tgaccgagag aagggccgaa gtgcctaccg cccaccctag cccatctcca 480
agacctgccg gccagttcca gacactcgtg                                  510

<210> 4
<211> 170
<212> PRT
<213> Artificial Sequence

<220> 
<223> PD1ECD

<400> 4
Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln
 1               5                  10                  15      
Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp
            20                  25                  30          
Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val
    50                  55                  60                  
Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala
65                  70                  75                  80  
Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg
                85                  90                  95      
Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg
            100                 105                 110         
Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu
        115                 120                 125             
Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val
    130                 135                 140                 
Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro
145                 150                 155                 160 
Arg Pro Ala Gly Gln Phe Gln Thr Leu Val
                165                 170 


<210> 5
<211> 170
<212> PRT
<213> Artificial Sequence

<220> 
<223> PD1ECD variant 1 A99L mutant

<400> 5
Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln
 1               5                  10                  15      
Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp
            20                  25                  30          
Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val
    50                  55                  60                  
Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala
65                  70                  75                  80  
Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg
                85                  90                  95      
Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg
            100                 105                 110         
Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu
        115                 120                 125             
Ala Pro Lys Leu Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val
    130                 135                 140                 
Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro
145                 150                 155                 160 
Arg Pro Ala Gly Gln Phe Gln Thr Leu Val
                165                 170 


<210> 6
<211> 170
<212> PRT
<213> Artificial Sequence

<220> 
<223> PD1ECD variant 2 mutant

<400> 6
Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln
 1               5                  10                  15      
Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp
            20                  25                  30          
Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp
        35                  40                  45              
Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe His
    50                  55                  60                  
Val Val Trp His Arg Glu Ser Pro Ser Gly Gln Thr Asp Thr Leu Ala
65                  70                  75                  80  
Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg
                85                  90                  95      
Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg
            100                 105                 110         
Ala Arg Arg Asn Asp Ser Gly Thr Tyr Val Cys Gly Val Ile Ser Leu
        115                 120                 125             
Ala Pro Lys Ile Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val
    130                 135                 140                 
Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro
145                 150                 155                 160 
Arg Pro Ala Gly Gln Phe Gln Thr Leu Val
                165                 170 


<210> 7
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> Glycine linker 1

<400> 7
Gly Gly Gly Ser
 1              


<210> 8
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Glycine linker 2

<400> 8
Gly Gly Gly Ser Gly Gly Gly
 1               5          


<210> 9
<211> 3
<212> PRT
<213> Artificial Sequence

<220> 
<223> Glycine linker 3

<400> 9
Gly Gly Gly
 1          


<210> 10
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A nucleotide sequence

<400> 10
ggcggcggag agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 60
cctagg                                                            66

<210> 11
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> GMCSF signal sequence

<400> 11
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atccca                                                            66

<210> 12
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> PD1 signal sequence

<400> 12
atgcagatcc ctcaggcccc ttggcctgtc gtgtgggctg tgctgcagct gggatggcgg 60


<210> 13
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD19 signal sequence

<400> 13
atgccccctc ccagacttct ctttttcctc ctcttcctga cgccgatgga agtaagg    57

<210> 14
<211> 2154
<212> DNA
<213> Artificial Sequence

<220> 
<223> EGFRt-T2A-PD1(A99L):IFNa2a

<400> 14
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atcccacgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata 120
aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc 180
ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa 240
ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct 300
gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag 360
caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc 420
tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat 480
gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata 540
agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc 600
cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga 660
ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag 720
aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc 780
acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc 840
gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca 900
gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca 960
ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg 1020
ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gggcggcgga 1080
gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctaggatg 1140
cagatccctc aggccccttg gcctgtcgtg tgggctgtgc tgcagctggg atggcggcct 1200
ggctggtttc tggacagccc cgacagaccc tggaaccccc ctacattttc ccctgccctg 1260
ctggtcgtga ccgagggcga caatgccacc ttcacctgta gcttcagcaa caccagcgag 1320
agcttcgtgc tgaactggta cagaatgagc cccagcaacc agaccgacaa gctggccgcc 1380
ttccccgagg atagatctca gcccggccag gactgccggt tcagagtgac ccagctgccc 1440
aacggccggg acttccacat gtctgtcgtg cgggccagac ggaacgacag cggcacatat 1500
ctgtgcggcg ccatcagcct ggcccccaag ctccagatca aagagagcct gagagccgag 1560
ctgagagtga ccgagagaag ggccgaagtg cctaccgccc accctagccc atctccaaga 1620
cctgccggcc agttccagac actcgtgggc ggaggatgcg acctgcctca gacacacagc 1680
ctgggcagca gacggaccct gatgctgctg gcccagatgc ggaagatcag cctgttcagc 1740
tgcctgaagg accggcacga cttcggcttc cctcaggaag agttcggcaa ccagtttcag 1800
aaggccgaga caatccccgt gctgcacgag atgatccagc agatcttcaa cctgttctcc 1860
accaaggaca gcagcgccgc ctgggacgag acactgctgg acaagttcta caccgagctg 1920
taccagcagc tgaatgacct ggaagcctgc gtgatccagg gcgtgggcgt gacagagaca 1980
cccctgatga aggaagatag catcctggcc gtgcgcaagt acttccagcg gatcaccctg 2040
tacctgaaag agaagaagta cagcccctgc gcctgggagg tcgtgcgcgc cgagatcatg 2100
agaagcttca gcctgagcac caacctgcag gaaagcctgc ggagcaaaga ataa       2154

<210> 15
<211> 717
<212> PRT
<213> Artificial Sequence

<220> 
<223> EGFRt-T2A-PD1(A99L):IFNa2a

<400> 15
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
 1               5                  10                  15      
Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly
            20                  25                  30          
Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe
        35                  40                  45              
Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala
    50                  55                  60                  
Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu
65                  70                  75                  80  
Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile
                85                  90                  95      
Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu
            100                 105                 110         
Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala
        115                 120                 125             
Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu
    130                 135                 140                 
Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr
145                 150                 155                 160 
Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys
                165                 170                 175     
Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly
            180                 185                 190         
Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu
        195                 200                 205             
Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys
    210                 215                 220                 
Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu
225                 230                 235                 240 
Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met
                245                 250                 255     
Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala
            260                 265                 270         
His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val
        275                 280                 285             
Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His
    290                 295                 300                 
Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro
305                 310                 315                 320 
Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala
                325                 330                 335     
Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly
            340                 345                 350         
Ile Gly Leu Phe Met Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr
        355                 360                 365             
Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Arg Met Gln Ile Pro Gln
    370                 375                 380                 
Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln Leu Gly Trp Arg Pro
385                 390                 395                 400 
Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe
                405                 410                 415     
Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr
            420                 425                 430         
Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg
        435                 440                 445             
Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp
    450                 455                 460                 
Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg Val Thr Gln Leu Pro
465                 470                 475                 480 
Asn Gly Arg Asp Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp
                485                 490                 495     
Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Leu Gln
            500                 505                 510         
Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala
        515                 520                 525             
Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly Gln
    530                 535                 540                 
Phe Gln Thr Leu Val Gly Gly Gly Cys Asp Leu Pro Gln Thr His Ser
545                 550                 555                 560 
Leu Gly Ser Arg Arg Thr Leu Met Leu Leu Ala Gln Met Arg Lys Ile
                565                 570                 575     
Ser Leu Phe Ser Cys Leu Lys Asp Arg His Asp Phe Gly Phe Pro Gln
            580                 585                 590         
Glu Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu Thr Ile Pro Val Leu
        595                 600                 605             
His Glu Met Ile Gln Gln Ile Phe Asn Leu Phe Ser Thr Lys Asp Ser
    610                 615                 620                 
Ser Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe Tyr Thr Glu Leu
625                 630                 635                 640 
Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Ile Gln Gly Val Gly
                645                 650                 655     
Val Thr Glu Thr Pro Leu Met Lys Glu Asp Ser Ile Leu Ala Val Arg
            660                 665                 670         
Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys Glu Lys Lys Tyr Ser
        675                 680                 685             
Pro Cys Ala Trp Glu Val Val Arg Ala Glu Ile Met Arg Ser Phe Ser
    690                 695                 700                 
Leu Ser Thr Asn Leu Gln Glu Ser Leu Arg Ser Lys Glu
705                 710                 715         


<210> 16
<211> 2154
<212> DNA
<213> Artificial Sequence

<220> 
<223> EGFRt-T2A-PD1(HAC-1):IFNa2a

<400> 16
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atcccacgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata 120
aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc 180
ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa 240
ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct 300
gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag 360
caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc 420
tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat 480
gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata 540
agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc 600
cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga 660
ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag 720
aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc 780
acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc 840
gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca 900
gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca 960
ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg 1020
ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gggcggcgga 1080
gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctaggatg 1140
cagatccctc aggccccttg gcctgtcgtg tgggctgtgc tgcagctggg atggcggcct 1200
ggctggtttc tggacagccc cgacagaccc tggaaccccc ctacattttc ccctgccctg 1260
ctggtcgtga ccgagggcga caatgccacc ttcacctgta gcttcagcaa caccagcgag 1320
agcttccatg ttatttggca tagagaaagc cccagcggtc agaccgacac tctggccgcc 1380
ttccccgagg atagatctca gcccggccag gactgccggt tcagagtgac ccagctgccc 1440
aacggccggg acttccacat gtctgtcgtg cgggccagac ggaacgacag cggcacatat 1500
gtttgcggcg ttatcagcct ggcccccaag attcagatca aagagagcct gagagccgag 1560
ctgagagtga ccgagagaag ggccgaagtg cctaccgccc accctagccc atctccaaga 1620
cctgccggcc agttccagac actcgtgggc ggaggatgcg acctgcctca gacacacagc 1680
ctgggcagca gacggaccct gatgctgctg gcccagatgc ggaagatcag cctgttcagc 1740
tgcctgaagg accggcacga cttcggcttc cctcaggaag agttcggcaa ccagtttcag 1800
aaggccgaga caatccccgt gctgcacgag atgatccagc agatcttcaa cctgttctcc 1860
accaaggaca gcagcgccgc ctgggacgag acactgctgg acaagttcta caccgagctg 1920
taccagcagc tgaatgacct ggaagcctgc gtgatccagg gcgtgggcgt gacagagaca 1980
cccctgatga aggaagatag catcctggcc gtgcgcaagt acttccagcg gatcaccctg 2040
tacctgaaag agaagaagta cagcccctgc gcctgggagg tcgtgcgcgc cgagatcatg 2100
agaagcttca gcctgagcac caacctgcag gaaagcctgc ggagcaaaga ataa       2154

<210> 17
<211> 2154
<212> DNA
<213> Artificial Sequence

<220> 
<223> EGFRt-T2A-PD1(G14-1):IFNa2a

<400> 17
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atcccacgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata 120
aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc 180
ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa 240
ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct 300
gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag 360
caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc 420
tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat 480
gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata 540
agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc 600
cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga 660
ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag 720
aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc 780
acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc 840
gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca 900
gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca 960
ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg 1020
ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gggcggcgga 1080
gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctaggatg 1140
cagatccctc aggccccttg gcctgtcgtg tgggctgtgc tgcagctggg atggcggcct 1200
ggctggtttc tggacagccc cgacagaccc tggaaccccc ctacattttc ccctgccctg 1260
ctggtcgtga ccgagggcga caatgccacc ttcacctgta gcttcagcaa caccagcgag 1320
agcttcagac tggtgtggca tagagaaagc cccggctacg agaccgacac tctggccagc 1380
ttccccgagg atagatctac ccccctgcct gactgccggt tcagagtgac ccagctgccc 1440
aacggccggg acttccacat gtctgtcgtg cgggccagac ggaacgacag cggcacatat 1500
gtttgcggcg ccatcgcctt ccaccccgtg attcagatca aagagagcct gagagccgag 1560
ctgagagtga ccgagagaag ggccgaagtg cctaccgccc accctagccc atctccaaga 1620
cctgccggcc agttccagac actcgtgggc ggaggatgcg acctgcctca gacacacagc 1680
ctgggcagca gacggaccct gatgctgctg gcccagatgc ggaagatcag cctgttcagc 1740
tgcctgaagg accggcacga cttcggcttc cctcaggaag agttcggcaa ccagtttcag 1800
aaggccgaga caatccccgt gctgcacgag atgatccagc agatcttcaa cctgttctcc 1860
accaaggaca gcagcgccgc ctgggacgag acactgctgg acaagttcta caccgagctg 1920
taccagcagc tgaatgacct ggaagcctgc gtgatccagg gcgtgggcgt gacagagaca 1980
cccctgatga aggaagatag catcctggcc gtgcgcaagt acttccagcg gatcaccctg 2040
tacctgaaag agaagaagta cagcccctgc gcctgggagg tcgtgcgcgc cgagatcatg 2100
agaagcttca gcctgagcac caacctgcag gaaagcctgc ggagcaaaga ataa       2154
