                         SEQUENCE LISTING

<110>  Deutsches Krebsforschungszentrum Stiftung des oeffentlichen Rechts
       Eberhard Karls Universitaet Tuebingen

<120>  Novel PSMA binding antibody and uses thereof

<130>  D31545WO

<150>  EP16151281.9
<151>  2016 01 14

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  murine 10B3 antibody heavy chain variable region

<400>  1

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Phe 
            20                  25                  30          


Tyr Met Tyr Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Asp Gly Gly Gly Tyr Thr Ser Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Ile Ala Lys Asn Asn Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Ser Glu Asp Thr Ala Lys Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Leu Trp Leu Arg Asp Ala Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Ser Val Thr Val Ser Ser 
        115                 


<210>  2
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  murine 10B3 antibody light chain variable region

<400>  2

Glu Ile Val Leu Thr Gln Ser Pro Ile Thr Met Ala Ala Phe Leu Gly 
1               5                   10                  15      


Glu Arg Ile Thr Ile Thr Cys Ser Ala Ser Ser Ser Ile Ser Ser Asn 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Phe Ser Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Arg Thr Ser Asn Leu Ala Ser Gly Val Pro Ile Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Gly Thr Met Glu 
65                  70                  75                  80  


Ala Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln Gly Ser Tyr Ile Pro 
                85                  90                  95      


Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105                 


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR-H1 of 10B3 antibody

<400>  3

Gly Phe Thr Phe Ser Asp Phe Tyr Met Tyr 
1               5                   10  


<210>  4
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR-H2 of 10B3 antibody

<400>  4

Thr Ile Ser Asp Gly Gly Gly Tyr Thr Ser Tyr Pro Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  5
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR-H3 of 10B3 antibody

<400>  5

Gly Leu Trp Leu Arg Asp Ala Leu Asp Tyr 
1               5                   10  


<210>  6
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR-L1 of 10B3 antibody

<400>  6

Ser Ala Ser Ser Ser Ile Ser Ser Asn Tyr Leu His 
1               5                   10          


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR-L2 of 10B3 antibody

<400>  7

Arg Thr Ser Asn Leu Ala Ser 
1               5           


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR-L3 of 10B3 antibody

<400>  8

Gln Gln Gly Ser Tyr Ile Pro Phe Thr 
1               5                   


<210>  9
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized 10B3 antibody heavy chain variable region

<400>  9

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Phe 
            20                  25                  30          


Tyr Met Tyr Trp Ile Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Asp Gly Gly Gly Tyr Thr Ser Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Leu Trp Leu Arg Asp Ala Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Val Thr Val Ser Ser 
        115                 


<210>  10
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized 10B3 antibody light chain variable region

<400>  10

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Ile Ser Ser Asn 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Arg Thr Ser Asn Leu Ala Ser Gly Ile Pro Ala Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Gly Ser Tyr Ile Pro 
                85                  90                  95      


Phe Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105                 


<210>  11
<211>  714
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain of h10B3xhUCHT1 bispecific IgGsc format antibody

<400>  11

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Ser Asp Phe Tyr Met Tyr Trp Ile Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ala Thr Ile Ser Asp Gly Gly Gly Tyr Thr Ser Tyr 
65                  70                  75                  80  


Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                85                  90                  95      


Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Gly Leu Trp Leu Arg Asp Ala Leu Asp Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
    130                 135                 140                 


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
145                 150                 155                 160 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
                165                 170                 175     


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
            180                 185                 190         


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
        195                 200                 205             


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
    210                 215                 220                 


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys 
225                 230                 235                 240 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val 
                245                 250                 255     


Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            260                 265                 270         


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Gly Val Ser 
        275                 280                 285             


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
    290                 295                 300                 


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
305                 310                 315                 320 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
                325                 330                 335     


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gln Leu Pro Ser Pro 
            340                 345                 350         


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
        355                 360                 365             


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
    370                 375                 380                 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
385                 390                 395                 400 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
                405                 410                 415     


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
            420                 425                 430         


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
        435                 440                 445             


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
    450                 455                 460                 


Ser Pro Gly Lys Ser Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
465                 470                 475                 480 


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser 
                485                 490                 495     


Gln Asp Ile Arg Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys 
            500                 505                 510         


Ala Pro Lys Leu Leu Ile Tyr Tyr Thr Ser Arg Leu Glu Ser Gly Val 
        515                 520                 525             


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr 
    530                 535                 540                 


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
545                 550                 555                 560 


Gly Asn Thr Leu Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
                565                 570                 575     


Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            580                 585                 590         


Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
        595                 600                 605             


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
    610                 615                 620                 


Thr Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
625                 630                 635                 640 


Ala Leu Ile Asn Pro Tyr Lys Gly Val Ser Thr Tyr Asn Gln Lys Phe 
                645                 650                 655     


Lys Asp Arg Phe Thr Ile Ser Val Asp Lys Ser Lys Asn Thr Ala Tyr 
            660                 665                 670         


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
        675                 680                 685             


Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp 
    690                 695                 700                 


Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
705                 710                 


<210>  12
<211>  606
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain of h10B3xOKT3 bispecific Fabsc format antibody

<400>  12

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Ser Asp Phe Tyr Met Tyr Trp Ile Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ala Thr Ile Ser Asp Gly Gly Gly Tyr Thr Ser Tyr 
65                  70                  75                  80  


Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                85                  90                  95      


Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Gly Leu Trp Leu Arg Asp Ala Leu Asp Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
    130                 135                 140                 


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
145                 150                 155                 160 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
                165                 170                 175     


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
            180                 185                 190         


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
        195                 200                 205             


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
    210                 215                 220                 


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys 
225                 230                 235                 240 


Ser Cys Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Pro Val 
                245                 250                 255     


Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            260                 265                 270         


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Gly Val Ser 
        275                 280                 285             


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
    290                 295                 300                 


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
305                 310                 315                 320 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
                325                 330                 335     


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gln Leu Pro Ser Pro 
            340                 345                 350         


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Ser Gly Gln Val 
        355                 360                 365             


Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala Ser Val 
    370                 375                 380                 


Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met 
385                 390                 395                 400 


His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr 
                405                 410                 415     


Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe Lys Asp 
            420                 425                 430         


Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 
        435                 440                 445             


Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg 
    450                 455                 460                 


Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Thr 
465                 470                 475                 480 


Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                485                 490                 495     


Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser 
            500                 505                 510         


Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser 
        515                 520                 525             


Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys 
    530                 535                 540                 


Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ala His 
545                 550                 555                 560 


Phe Arg Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Gly 
                565                 570                 575     


Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser 
            580                 585                 590         


Asn Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Asn 
        595                 600                 605     


<210>  13
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain of humanized 10B3 antibody

<400>  13

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser 
            20                  25                  30          


Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser 
        35                  40                  45              


Ile Ser Ser Asn Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala 
    50                  55                  60                  


Pro Arg Leu Leu Ile Tyr Arg Thr Ser Asn Leu Ala Ser Gly Ile Pro 
65                  70                  75                  80  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile 
                85                  90                  95      


Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Gly 
            100                 105                 110         


Ser Tyr Ile Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125             


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
    130                 135                 140                 


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
145                 150                 155                 160 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
                165                 170                 175     


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
            180                 185                 190         


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
        195                 200                 205             


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
    210                 215                 220                 


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235 


<210>  14
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated CDR-H1 of 10B3 antibody

<400>  14

Gly Phe Ser Phe Thr Asp Phe Tyr Met Tyr 
1               5                   10  


<210>  15
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variable heavy chain domain of J519 antibody

<400>  15

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Thr 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Glu Tyr 
            20                  25                  30          


Thr Ile His Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Asn Pro Asn Asn Gly Gly Thr Thr Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Glu Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gly Trp Asn Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  16
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variable light chain domain Jof J519 antibody

<400>  16

Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Ile Ile Cys Lys Ala Ser Gln Asp Val Gly Thr Ala 
            20                  25                  30          


Val Asp Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Ala Ile Thr Asn Val Gln Ser 
65                  70                  75                  80  


Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             





