                         SEQUENCE LISTING

<110>  Keygene N.V.
 
<120>  METHOD

<130>  P108830PCT

<150>  GB 1601046.4
<151>  2016-01-20

<150>  GB 1600765.0
<151>  2016-01-15

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1387
<212>  DNA
<213>  Nicotiana tabacum

<400>  1
tgcaaattct gctttctttt catatcattc tcaaattctt ccattttgaa ttcacactcc       60

tttctttcaa atcattcaaa atgaatgacc ttatgaccaa atctttcaca agctacgttg      120

atttgaagaa agcagcgatg aaagatgttg aagctggtcc agatttggaa atgggtatga      180

cccaaattga ccaaaatctc aatgcttttc tagaagaagc agagaaggta aaactagaaa      240

tgaattcaat caaagatatc cttcgccgtt tacaagacac caacgaagaa agcaagtcac      300

tgcacaaacc cgaagctttg aaatctatgc gaaatcgcat aaactctgat attctagttg      360

tgttaaagaa ggctagagct attaggtctc agctagaaga aatggaccgg tctaatgcaa      420

taaaccggcg gctttctggg tgtaaagaag gtacaccggt tgataggaca cggtctgctg      480

ttacaaatgg gcttaggaaa aagcttaagg agctaatgat tgattttcag gggctaaggc      540

agaggatgat gactgagtat aaagaaactg ttgggagaag atattttact gtgactggtg      600

agcacccaga tgaagaagtc attgaaaaga tcatttctag tggcaatggt caaggtggtg      660

aagaatttct ttctagagca attcaggtat acccaaaata atgccaaaaa tatatgcctt      720

ttgcctaatt tctctctgtt tctagaccta attgataagg aagattctta tagccaacca      780

aactaaactg aacttttgaa gcatagttgt tattattgtc ataaagaatt attgactaac      840

aaactttggt gggtgggttg caggagcatg ggagggggaa ggtattggaa acagtggtag      900

agatacagga ccgccacgac gcggccaagg agatagaaaa gagcttgctg gagctgcacc      960

agatattctt ggacatggca gtgatggtag agatacaagg agagaaaatg gatgacattg     1020

agcaccatgt gatgaatgca gcacagtatg ttaatgatgg aactaagaat ctcaagactg     1080

caaaagacta ccagaagagc agcagaaaat ggatgtgcat tgccattata attctcctaa     1140

ttctcatcct tgtagttatc atccccattg ctaccagttt cagcaaatct tgaagtgtag     1200

gccccattgt gataatcagc tgttatttca ttcttatatt cttttttagc aattactttt     1260

tgtcactctc gtgagaatta tgtatggtta tggtacaagg ctgttagtat tacatatacc     1320

ttgtgacatg atgtaagaac ctttaataga ttttgcttca gaaacatttt cttctgtttt     1380

ctgaatt                                                               1387


<210>  2
<211>  936
<212>  DNA
<213>  Nicotiana tabacum

<400>  2
atgaatgacc ttatgaccaa atctttcaca agctacgttg atttgaagaa agcagcgatg       60

aaagatgttg aagctggtcc agatttggaa atgggtatga cccaaattga ccaaaatctc      120

aatgcttttc tagaagaagc agagaaggta aaactagaaa tgaattcaat caaagatatc      180

cttcgccgtt tacaagacac caacgaagaa agcaagtcac tgcacaaacc cgaagctttg      240

aaatctatgc gaaatcgcat aaactctgat attctagttg tgttaaagaa ggctagagct      300

attaggtctc agctagaaga aatggaccgg tctaatgcaa taaaccggcg gctttctggg      360

tgtaaagaag gtacaccggt tgataggaca cggtctgctg ttacaaatgg gcttaggaaa      420

aagcttaagg agctaatgat tgattttcag gggctaaggc agaggatgat gactgagtat      480

aaagaaactg ttgggagaag atattttact gtgactggtg agcacccaga tgaagaagtc      540

attgaaaaga tcatttctag tggcaatggt caaggtggtg aagaatttct ttctagagca      600

attcaggagc atgggagggg gaaggtattg gaaacagtgg tagagataca ggaccgccac      660

gacgcggcca aggagataga aaagagcttg ctggagctgc accagatatt cttggacatg      720

gcagtgatgg tagagataca aggagagaaa atggatgaca ttgagcacca tgtgatgaat      780

gcagcacagt atgttaatga tggaactaag aatctcaaga ctgcaaaaga ctaccagaag      840

agcagcagaa aatggatgtg cattgccatt ataattctcc taattctcat ccttgtagtt      900

atcatcccca ttgctaccag tttcagcaaa tcttga                                936


<210>  3
<211>  311
<212>  PRT
<213>  Nicotiana tabacum

<400>  3

Met Asn Asp Leu Met Thr Lys Ser Phe Thr Ser Tyr Val Asp Leu Lys 
1               5                   10                  15      


Lys Ala Ala Met Lys Asp Val Glu Ala Gly Pro Asp Leu Glu Met Gly 
            20                  25                  30          


Met Thr Gln Ile Asp Gln Asn Leu Asn Ala Phe Leu Glu Glu Ala Glu 
        35                  40                  45              


Lys Val Lys Leu Glu Met Asn Ser Ile Lys Asp Ile Leu Arg Arg Leu 
    50                  55                  60                  


Gln Asp Thr Asn Glu Glu Ser Lys Ser Leu His Lys Pro Glu Ala Leu 
65                  70                  75                  80  


Lys Ser Met Arg Asn Arg Ile Asn Ser Asp Ile Leu Val Val Leu Lys 
                85                  90                  95      


Lys Ala Arg Ala Ile Arg Ser Gln Leu Glu Glu Met Asp Arg Ser Asn 
            100                 105                 110         


Ala Ile Asn Arg Arg Leu Ser Gly Cys Lys Glu Gly Thr Pro Val Asp 
        115                 120                 125             


Arg Thr Arg Ser Ala Val Thr Asn Gly Leu Arg Lys Lys Leu Lys Glu 
    130                 135                 140                 


Leu Met Ile Asp Phe Gln Gly Leu Arg Gln Arg Met Met Thr Glu Tyr 
145                 150                 155                 160 


Lys Glu Thr Val Gly Arg Arg Tyr Phe Thr Val Thr Gly Glu His Pro 
                165                 170                 175     


Asp Glu Glu Val Ile Glu Lys Ile Ile Ser Ser Gly Asn Gly Gln Gly 
            180                 185                 190         


Gly Glu Glu Phe Leu Ser Arg Ala Ile Gln Glu His Gly Arg Gly Lys 
        195                 200                 205             


Val Leu Glu Thr Val Val Glu Ile Gln Asp Arg His Asp Ala Ala Lys 
    210                 215                 220                 


Glu Ile Glu Lys Ser Leu Leu Glu Leu His Gln Ile Phe Leu Asp Met 
225                 230                 235                 240 


Ala Val Met Val Glu Ile Gln Gly Glu Lys Met Asp Asp Ile Glu His 
                245                 250                 255     


His Val Met Asn Ala Ala Gln Tyr Val Asn Asp Gly Thr Lys Asn Leu 
            260                 265                 270         


Lys Thr Ala Lys Asp Tyr Gln Lys Ser Ser Arg Lys Trp Met Cys Ile 
        275                 280                 285             


Ala Ile Ile Ile Leu Leu Ile Leu Ile Leu Val Val Ile Ile Pro Ile 
    290                 295                 300                 


Ala Thr Ser Phe Ser Lys Ser 
305                 310     


<210>  4
<211>  311
<212>  PRT
<213>  Solanum lycopersicum

<400>  4

Met Asn Asp Leu Met Thr Lys Ser Phe Thr Ser Tyr Ile Asp Leu Lys 
1               5                   10                  15      


Lys Ala Ala Met Lys Asp Val Glu Ala Ser Pro Asp Leu Glu Met Gly 
            20                  25                  30          


Met Thr Gln Met Asp Gln Asn Leu Thr Ala Phe Leu Glu Glu Ala Glu 
        35                  40                  45              


Lys Val Lys Leu Glu Met Asn Ser Ile Lys Glu Ile Leu Arg Arg Leu 
    50                  55                  60                  


Gln Asp Thr Asn Glu Glu Ser Lys Ser Leu His Lys Pro Glu Ala Leu 
65                  70                  75                  80  


Lys Ser Met Arg Asp Arg Ile Asn Ser Asp Ile Val Ala Val Leu Lys 
                85                  90                  95      


Lys Ala Arg Ala Ile Arg Ser Gln Leu Glu Glu Met Asp Arg Ser Asn 
            100                 105                 110         


Ala Ile Asn Arg Arg Leu Ser Gly Cys Lys Glu Gly Thr Leu Val Asp 
        115                 120                 125             


Arg Thr Arg Ser Ala Val Thr Asn Gly Leu Arg Lys Lys Leu Lys Glu 
    130                 135                 140                 


Leu Met Met Asp Phe Gln Gly Leu Arg Gln Arg Met Met Thr Glu Tyr 
145                 150                 155                 160 


Lys Glu Thr Val Gly Arg Arg Tyr Phe Thr Val Thr Gly Glu His Pro 
                165                 170                 175     


Asp Glu Glu Val Ile Asp Lys Ile Ile Ser Ser Gly Asn Gly Gln Gly 
            180                 185                 190         


Gly Glu Glu Phe Leu Ser Arg Ala Ile Gln Glu His Gly Arg Gly Lys 
        195                 200                 205             


Val Leu Glu Thr Val Val Glu Ile Gln Asp Arg His Asp Ala Ala Lys 
    210                 215                 220                 


Glu Ile Glu Lys Ser Leu Leu Glu Leu His Gln Ile Phe Leu Asp Met 
225                 230                 235                 240 


Ala Val Met Val Glu Ala Gln Gly Glu Lys Met Asp Asp Ile Glu His 
                245                 250                 255     


His Val Val Asn Ala Ala Gln Tyr Val Asn Asp Gly Ala Lys Asn Leu 
            260                 265                 270         


Lys Thr Ala Lys Lys Tyr Gln Lys Ser Ser Arg Arg Cys Met Cys Ile 
        275                 280                 285             


Gly Ala Ile Ile Leu Leu Ile Leu Ile Leu Val Val Ile Ile Pro Ile 
    290                 295                 300                 


Ala Thr Ser Phe Thr Lys Ser 
305                 310     


<210>  5
<211>  311
<212>  PRT
<213>  Solanum tuberosum

<400>  5

Met Asn Asp Leu Met Thr Lys Ser Phe Thr Ser Tyr Ile Asp Leu Lys 
1               5                   10                  15      


Lys Ala Ala Met Lys Asp Val Glu Ala Ser Pro Asp Leu Glu Met Gly 
            20                  25                  30          


Met Thr Gln Leu Asp Gln Asn Leu Thr Ala Phe Leu Glu Glu Ala Glu 
        35                  40                  45              


Lys Val Lys Leu Glu Met Asn Ser Ile Lys Glu Ile Leu Arg Arg Leu 
    50                  55                  60                  


Gln Asp Thr Asn Glu Glu Ser Lys Ser Leu His Lys Pro Glu Ala Leu 
65                  70                  75                  80  


Lys Ser Met Arg Asp Ser Ile Asn Ser Asp Ile Val Ala Val Leu Lys 
                85                  90                  95      


Lys Ala Arg Ala Ile Arg Ser Gln Leu Glu Glu Met Asp Arg Ser Asn 
            100                 105                 110         


Ala Ile Asn Arg Arg Leu Ser Gly Cys Lys Glu Gly Thr Leu Val Asp 
        115                 120                 125             


Arg Thr Arg Ser Ala Val Thr Asn Gly Leu Arg Lys Lys Leu Lys Glu 
    130                 135                 140                 


Leu Met Met Glu Phe Gln Gly Leu Arg Gln Arg Met Met Thr Glu Tyr 
145                 150                 155                 160 


Lys Glu Thr Val Gly Arg Arg Tyr Phe Thr Val Thr Gly Glu His Pro 
                165                 170                 175     


Asp Glu Glu Val Ile Asp Lys Ile Ile Ser Ser Gly Asn Gly Gln Gly 
            180                 185                 190         


Gly Glu Glu Phe Leu Ser Arg Ala Ile Gln Glu His Gly Arg Gly Lys 
        195                 200                 205             


Val Leu Glu Thr Val Val Glu Ile Gln Asp Arg His Asp Ala Ala Lys 
    210                 215                 220                 


Glu Ile Glu Arg Ser Leu Leu Glu Leu His Gln Ile Phe Leu Asp Met 
225                 230                 235                 240 


Ala Val Met Val Glu Ala Gln Gly Glu Lys Met Asp Asp Ile Glu His 
                245                 250                 255     


His Val Val Asn Ala Ala His Tyr Val Asn Asp Gly Ala Lys Asn Leu 
            260                 265                 270         


Lys Thr Ala Lys Lys Tyr Gln Lys Ser Ser Arg Lys Cys Met Phe Ile 
        275                 280                 285             


Gly Val Ile Val Leu Leu Ile Leu Ile Leu Val Val Ile Ile Pro Ile 
    290                 295                 300                 


Ala Thr Ser Phe Thr Lys Ser 
305                 310     


<210>  6
<211>  936
<212>  DNA
<213>  Solanum lycopersicum

<400>  6
atgaatgatt tgatgacaaa atccttcaca agctacattg atctgaagaa agccgccatg       60

aaagatgttg aagctagtcc agatttggaa atgggtatga cccaaatgga tcaaaatctc      120

actgctttct tagaagaagc agaaaaagtg aaattggaga tgaattcaat caaggagatt      180

cttcgtcggt tacaggacac taatgaagaa agcaagtcgc tgcacaaacc cgaagctttg      240

aaatcgatgc gtgatcgcat aaattcagat atcgtagcgg tgttgaagaa ggctagagct      300

attagatctc agctggaaga gatggaccga tccaatgcga ttaacaggcg gctttctggg      360

tgtaaagaag ggacgctggt cgataggact cgatctgctg tgactaatgg gctgaggaag      420

aagcttaagg aactgatgat ggattttcag ggactgaggc agaggatgat gactgagtat      480

aaggaaactg ttggaagaag atattttact gttactggtg aacacccaga tgaagaagtt      540

attgataaga tcatttctag tggaaatggt caaggtggtg aagaatttct ttctagagca      600

attcaggagc atggtagggg gaaagtgttg gaaacagtgg tggagataca ggaccgtcat      660

gacgcagcaa aggagatcga aaagagcttg cttgagcttc accagatatt cttggacatg      720

gcagtgatgg ttgaggcaca aggagagaaa atggatgaca ttgaacatca tgtagtgaat      780

gcagctcagt atgttaatga tggagctaag aacctcaaga ctgcaaagaa gtatcaaaag      840

agcagcagga gatgtatgtg cattggagct ataattctcc ttattctcat cctggtagtc      900

attatcccca ttgccaccag tttcaccaaa tcttga                                936


<210>  7
<211>  936
<212>  DNA
<213>  Solanum tuberosum

<400>  7
atgaatgatt tgatgacaaa atctttcaca agctacattg atctgaagaa agccgccatg       60

aaagatgttg aagctagtcc agatttggaa atgggtatga cccaattgga tcaaaatctc      120

actgctttct tagaagaagc agaaaaggtg aaattggaga tgaattcaat caaggagatt      180

cttcgtcggt tacaggacac taatgaagaa agcaagtcgc tacacaaacc tgaagctttg      240

aaatcgatgc gtgatagcat aaattcagat attgtagctg tgttgaagaa ggctagagct      300

attagatctc agctggaaga gatggaccga tccaatgcga ttaacaggcg gctttctggg      360

tgtaaagaag ggacattggt cgataggact cgatctgctg taactaatgg gcttaggaag      420

aagcttaagg aacttatgat ggagtttcag ggactgaggc agaggatgat gactgagtat      480

aaggagactg ttggaagaag atattttact gttactggtg aacacccaga tgaagaagtt      540

attgacaaga tcatttctag tggaaatggt caaggtggtg aagaatttct ttctagagca      600

attcaggagc atgggagggg gaaggtgttg gaaacagtgg tggagataca ggaccgtcat      660

gacgcagcaa aggagataga aaggagcttg cttgagctgc accagatatt cttggacatg      720

gcagtgatgg ttgaggcaca aggagagaaa atggatgata ttgaacacca tgtggtgaat      780

gcagcccatt atgttaatga tggagccaag aacctcaaga ctgcaaagaa gtatcaaaag      840

agcagcagga aatgtatgtt cattggagtt atagttctcc taattctgat cctggtagtc      900

atcatcccca ttgccaccag tttcaccaaa tcttga                                936


<210>  8
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant TFA0069 mature protein

<400>  8

Met Asn Asp Leu Met Thr Lys Ser Phe Thr Ser Tyr Val Asp Leu Lys 
1               5                   10                  15      


Lys Ala Ala Met Lys Asp Val Glu Ala Gly Pro Asp Leu Glu Met Gly 
            20                  25                  30          


Met Thr Gln Ile Asp Gln Asn Leu Asn Ala Phe Leu Glu Glu Ala Glu 
        35                  40                  45              


Lys Val Lys Leu Glu Met Asn Ser Ile Lys Asp Ile Leu Arg Arg Leu 
    50                  55                  60                  


Gln Asp Thr Asn Glu Glu Ser Lys Ser Leu His Lys Pro Glu Ala Leu 
65                  70                  75                  80  


Lys Ser Met 
            


