                         SEQUENCE LISTING

<110>  Conagen Inc.
 
<120>  METHODS OF MAKING CAPSINOIDS BY BIOSYNTHETIC PROCESSES

<130>  C1497.70003WO00

<150>  US 62/276059
<151>  2016-01-07

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  440
<212>  PRT
<213>  Capsicum annuum L

<400>  1

Met Ala Phe Ala Leu Pro Ser Ser Leu Val Ser Val Cys Asp Lys Ser 
1               5                   10                  15      


Phe Ile Lys Pro Ser Ser Leu Thr Pro Ser Lys Leu Arg Phe His Lys 
            20                  25                  30          


Leu Ser Phe Ile Asp Gln Ser Leu Ser Asn Met Tyr Ile Pro Cys Ala 
        35                  40                  45              


Phe Phe Tyr Pro Lys Val Gln Gln Arg Leu Glu Asp Ser Lys Asn Ser 
    50                  55                  60                  


Asp Glu Leu Ser His Ile Ala His Leu Leu Gln Thr Ser Leu Ser Gln 
65                  70                  75                  80  


Thr Leu Val Ser Tyr Tyr Pro Tyr Ala Gly Lys Leu Lys Asp Asn Ala 
                85                  90                  95      


Thr Val Asp Cys Asn Asp Met Gly Ala Glu Phe Leu Ser Val Arg Ile 
            100                 105                 110         


Lys Cys Ser Met Ser Glu Ile Leu Asp His Pro His Ala Ser Leu Ala 
        115                 120                 125             


Glu Ser Ile Val Leu Pro Lys Asp Leu Pro Trp Ala Asn Asn Cys Glu 
    130                 135                 140                 


Gly Gly Asn Leu Leu Val Val Gln Val Ser Lys Phe Asp Cys Gly Gly 
145                 150                 155                 160 


Ile Ala Ile Ser Val Cys Phe Ser His Lys Ile Gly Asp Gly Cys Ser 
                165                 170                 175     


Leu Leu Asn Phe Leu Asn Asp Trp Ser Ser Val Thr Arg Asp His Thr 
            180                 185                 190         


Thr Thr Ala Leu Val Pro Ser Pro Arg Phe Val Gly Asp Ser Val Phe 
        195                 200                 205             


Ser Thr Lys Lys Tyr Gly Ser Leu Ile Thr Pro Gln Ile Leu Ser Asp 
    210                 215                 220                 


Leu Asn Glu Cys Val Gln Lys Arg Leu Ile Phe Pro Thr Asp Lys Leu 
225                 230                 235                 240 


Asp Ala Leu Arg Ala Lys Val Ala Glu Glu Ser Gly Val Lys Asn Pro 
                245                 250                 255     


Thr Arg Ala Glu Val Val Ser Ala Leu Leu Phe Lys Cys Ala Thr Lys 
            260                 265                 270         


Ala Ser Ser Ser Met Leu Pro Ser Lys Leu Val His Phe Leu Asn Ile 
        275                 280                 285             


Arg Thr Met Ile Lys Pro Arg Leu Pro Arg Asn Ala Ile Gly Asn Leu 
    290                 295                 300                 


Ser Ser Ile Phe Ser Ile Glu Ala Thr Asn Met Gln Asp Met Glu Leu 
305                 310                 315                 320 


Pro Thr Leu Val Arg Asn Leu Arg Lys Glu Val Glu Val Ala Tyr Lys 
                325                 330                 335     


Lys Asp Gln Val Glu Gln Asn Glu Leu Ile Leu Glu Val Val Glu Ser 
            340                 345                 350         


Met Arg Glu Gly Lys Leu Pro Phe Glu Asn Met Asp Gly Tyr Glu Asn 
        355                 360                 365             


Val Tyr Thr Cys Ser Asn Leu Cys Lys Tyr Pro Tyr Tyr Thr Val Asp 
    370                 375                 380                 


Phe Gly Trp Gly Arg Pro Glu Arg Val Cys Leu Gly Asn Gly Pro Ser 
385                 390                 395                 400 


Lys Asn Ala Phe Phe Leu Lys Asp Tyr Lys Ala Gly Gln Gly Val Glu 
                405                 410                 415     


Ala Arg Val Met Leu His Lys Gln Gln Met Ser Glu Phe Glu Arg Asn 
            420                 425                 430         


Glu Glu Leu Leu Glu Phe Ile Ala 
        435                 440 


<210>  2
<211>  658
<212>  PRT
<213>  Capsicum annuum L.

<400>  2

Met Ala Thr Asp Lys Phe Ile Ile Glu Val Glu Ser Ala Lys Pro Ala 
1               5                   10                  15      


Lys Asp Gly Arg Pro Ser Met Gly Pro Val Tyr Arg Ser Ile Phe Ala 
            20                  25                  30          


Lys His Gly Phe Pro Pro Pro Ile Pro Gly Leu Asp Ser Cys Trp Asp 
        35                  40                  45              


Ile Phe Arg Met Ser Val Glu Lys Tyr Pro Asn Asn Arg Met Leu Gly 
    50                  55                  60                  


Arg Arg Glu Ile Val Asp Gly Lys Pro Gly Lys Tyr Val Trp Met Ser 
65                  70                  75                  80  


Tyr Lys Glu Val Tyr Asp Ile Val Ile Lys Val Gly Asn Ser Ile Arg 
                85                  90                  95      


Ser Ile Gly Val Asp Val Gly Asp Lys Cys Gly Ile Tyr Gly Ala Asn 
            100                 105                 110         


Cys Pro Glu Trp Ile Ile Ser Met Glu Ala Cys Asn Ala His Gly Leu 
        115                 120                 125             


Tyr Cys Val Pro Leu Tyr Asp Thr Leu Gly Ala Gly Ala Val Glu Phe 
    130                 135                 140                 


Ile Ile Ser His Ala Glu Val Thr Ile Ala Phe Val Glu Glu Lys Lys 
145                 150                 155                 160 


Leu Pro Glu Leu Leu Lys Thr Phe Pro Asn Ala Ser Lys Tyr Leu Lys 
                165                 170                 175     


Thr Ile Val Ser Phe Gly Lys Val Thr Pro Glu Gln Lys Lys Glu Leu 
            180                 185                 190         


Glu Glu Phe Gly Val Val Leu Tyr Ser Trp Asp Glu Phe Leu Gln Leu 
        195                 200                 205             


Gly Ser Gly Lys Gln Phe Asp Leu Pro Val Lys Lys Lys Glu Asp Ile 
    210                 215                 220                 


Cys Thr Ile Met Tyr Thr Ser Gly Thr Thr Gly Asp Pro Lys Gly Val 
225                 230                 235                 240 


Leu Ile Ser Asn Thr Ser Ile Val Thr Leu Ile Ala Gly Val Arg Arg 
                245                 250                 255     


Phe Leu Gly Ser Val Asp Glu Ser Leu Asn Val Asp Asp Val Tyr Leu 
            260                 265                 270         


Ser Tyr Leu Pro Leu Ala His Ile Phe Asp Arg Val Ile Glu Glu Cys 
        275                 280                 285             


Phe Ile His His Gly Ala Ser Ile Gly Phe Trp Arg Gly Asp Val Lys 
    290                 295                 300                 


Leu Leu Thr Glu Asp Ile Gly Glu Leu Lys Pro Thr Val Phe Cys Ala 
305                 310                 315                 320 


Val Pro Arg Val Leu Asp Arg Ile Tyr Ser Gly Leu Gln Gln Lys Ile 
                325                 330                 335     


Ala Ala Gly Gly Phe Leu Lys Ser Thr Leu Phe Asn Leu Ala Tyr Ala 
            340                 345                 350         


Tyr Lys His His Asn Leu Lys Lys Gly Arg Lys His Phe Glu Ala Ser 
        355                 360                 365             


Pro Leu Ser Asp Lys Val Val Phe Ser Lys Val Lys Glu Gly Leu Gly 
    370                 375                 380                 


Gly Arg Val Arg Leu Ile Leu Ser Gly Ala Ala Pro Leu Ala Ala His 
385                 390                 395                 400 


Val Glu Ala Phe Leu Arg Val Val Ala Cys Cys His Val Leu Gln Gly 
                405                 410                 415     


Tyr Gly Leu Thr Glu Thr Cys Ala Gly Thr Phe Val Ser Leu Pro Asn 
            420                 425                 430         


Arg Tyr Asp Met Leu Gly Thr Val Gly Pro Pro Val Pro Asn Val Asp 
        435                 440                 445             


Val Cys Leu Glu Ser Val Pro Glu Met Ser Tyr Asp Ala Leu Ser Ser 
    450                 455                 460                 


Thr Pro Arg Gly Glu Val Cys Val Arg Gly Asp Val Leu Phe Ser Gly 
465                 470                 475                 480 


Tyr Tyr Lys Arg Glu Asp Leu Thr Lys Glu Val Met Ile Asp Gly Trp 
                485                 490                 495     


Phe His Thr Gly Asp Val Gly Glu Trp Gln Pro Asn Gly Ser Leu Lys 
            500                 505                 510         


Ile Ile Asp Arg Lys Lys Asn Ile Phe Lys Leu Ser Gln Gly Glu Tyr 
        515                 520                 525             


Val Ala Val Glu Asn Leu Glu Asn Ile Tyr Gly Asn Asn Pro Ile Ile 
    530                 535                 540                 


Asp Ser Ile Trp Ile Tyr Gly Asn Ser Phe Glu Ser Phe Leu Val Ala 
545                 550                 555                 560 


Val Ile Asn Pro Asn Gln Arg Ala Val Glu Gln Trp Ala Glu Val Asn 
                565                 570                 575     


Gly Leu Ser Gly Asp Phe Ala Ser Leu Cys Glu Lys Pro Glu Val Lys 
            580                 585                 590         


Glu Tyr Ile Leu Arg Glu Leu Thr Lys Thr Gly Lys Glu Lys Lys Leu 
        595                 600                 605             


Lys Gly Phe Glu Phe Leu Lys Ala Val His Leu Asp Pro Val Pro Phe 
    610                 615                 620                 


Asp Met Glu Arg Asp Leu Leu Thr Pro Thr Phe Lys Lys Lys Arg Pro 
625                 630                 635                 640 


Gln Leu Leu Lys Tyr Tyr Lys Asp Val Ile Asp Ser Met Tyr Lys Gly 
                645                 650                 655     


Thr Lys 
        


<210>  3
<211>  1323
<212>  DNA
<213>  Capsicum annuum L.

<400>  3
atggcttttg cattaccatc atcacttgtt tcagtttgtg acaaatcttt tatcaaacct       60

tcctctctca ccccctctaa acttagattt cacaagctat ctttcatcga tcaatcttta      120

agtaatatgt atatcccttg tgcatttttt taccctaaag tacaacaaag actagaagac      180

tccaaaaatt ctgatgagct ttcccatata gcccacttgc tacaaacatc tctatcacaa      240

actctagtct cttactatcc ttatgcagga aagttgaagg acaatgctac tgttgactgt      300

aacgatatgg gagctgagtt cttgagtgtt cgaataaaat gttccatgtc tgaaattctt      360

gatcatcctc atgcatctct tgcagagagc atagttttgc ccaaggattt gccttgggcg      420

aataattgtg aaggtggtaa tttgcttgta gttcaagtaa gtaagtttga ttgtggggga      480

atagccatca gtgtatgctt ttcgcacaag attggtgatg gttgctctct gcttaatttc      540

cttaatgatt ggtctagcgt tactcgtgat catacgacaa cagctttagt tccatctcct      600

agatttgtag gagattctgt cttctctaca aaaaaatatg gttctcttat tacgccacaa      660

attttgtccg atctcaacga gtgcgtacag aaaagactca tttttcctac agataagtta      720

gatgcacttc gagctaaggt ggcagaagaa tcaggagtaa aaaatccaac aagggcagaa      780

gttgttagcg ctcttctttt caaatgtgca acaaaggcat catcatcaat gctaccatca      840

aagttggttc acttcttaaa catacgtact atgatcaaac ctcgtctacc acgaaatgcc      900

attggaaatc tctcgtctat tttctccata gaagcaacta acatgcagga catggagttg      960

ccaacgttgg ttcgtaattt aaggaaggaa gttgaggtgg catacaagaa agaccaagtc     1020

gaacaaaatg aactgatcct agaagtagta gaatcaatga gagaagggaa actgccattt     1080

gaaaatatgg atggctatga gaatgtgtat acttgcagca atctttgcaa atatccgtac     1140

tacactgtag attttggatg gggaagacct gaaagagtgt gtctaggaaa tggtccctcc     1200

aagaatgcct tcttcttgaa agattacaaa gctgggcaag gcgtggaggc gcgggtgatg     1260

ttgcacaagc aacaaatgtc tgaatttgaa cgcaatgagg aactccttga gttcattgcc     1320

taa                                                                   1323


<210>  4
<211>  1977
<212>  DNA
<213>  Capsicum annuum L.

<400>  4
atggctacgg acaaatttat tattgaagtt gaatcagcaa aaccggcaaa agatggtcgc       60

ccgagcatgg gcccggtcta tcgttcgatc tttgcgaaac atggctttcc gccgccgatc      120

ccgggtctgg attcatgctg ggacattttt cgtatgtcgg tggaaaaata tccgaacaat      180

cgcatgctgg gccgtcgcga aattgttgat ggcaaaccgg gtaaatacgt ttggatgagc      240

tacaaagaag tctacgacat cgttatcaaa gtcggtaaca gtattcgttc catcggcgtg      300

gatgttggtg acaaatgcgg catttatggt gcaaactgtc cggaatggat tatcagcatg      360

gaagcatgca atgctcatgg cctgtattgt gtcccgctgt acgataccct gggcgcaggt      420

gctgtggaat ttattatctc tcacgcggaa gtgaccatcg ccttcgttga agagaaaaaa      480

ctgccggaac tgctgaaaac ctttccgaat gcgagcaaat atctgaaaac cattgtctct      540

ttcggcaaag tgacgccgga acagaagaaa gaactggaag aatttggtgt ggttctgtac      600

agttgggatg aatttctgca gctgggctcc ggtaaacaat tcgatctgcc ggtgaaaaag      660

aaagaagata tttgcaccat catgtatacg agcggcacca cgggtgatcc gaaaggtgtg      720

ctgatttcaa acacctcgat tgtgacgctg atcgccggtg ttcgtcgctt tctgggctca      780

gttgatgaat cgctgaatgt ggatgacgtt tatctgtcat acctgccgct ggcacatatt      840

tttgaccgtg tgatcgaaga atgcttcatt catcacggcg cttcgatcgg tttttggcgc      900

ggcgatgtga aactgctgac cgaagacatt ggcgaactga aaccgacggt tttctgtgcg      960

gtcccgcgtg tgctggatcg catctattca ggtctgcagc aaaaaattgc ggccggcggt     1020

tttctgaaat cgaccctgtt caacctggcg tatgcctaca aacatcacaa tctgaagaaa     1080

ggccgcaaac actttgaagc cagcccgctg tctgataaag tcgtgttcag taaagtgaaa     1140

gaaggcctgg gcggtcgtgt tcgcctgatt ctgtccggtg cggctccgct ggccgcacat     1200

gtggaagcgt ttctgcgtgt tgtcgcctgc tgtcacgttc tgcagggcta tggtctgacc     1260

gaaacgtgcg caggcacctt cgtgagtctg ccgaaccgct acgatatgct gggcacggtt     1320

ggtccgccgg tcccgaatgt cgatgtgtgc ctggaaagcg tgccggaaat gtcttatgac     1380

gctctgagct ctaccccgcg tggtgaagtt tgtgtccgcg gcgatgttct gttttccggt     1440

tattacaaac gtgaagacct gaccaaagaa gttatgattg atggctggtt ccatacgggc     1500

gacgtcggtg aatggcagcc gaacggtagc ctgaaaatca tcgatcgtaa gaaaaacatc     1560

ttcaaactgt ctcaaggcga atatgtggcc gttgaaaacc tggaaaatat ttacggcaac     1620

aatccgatta tcgacagcat ttggatctat ggtaacagtt ttgaatcctt cctggtcgcg     1680

gtgatcaacc cgaatcagcg tgcagtcgaa caatgggctg aagtgaatgg cctgagtggt     1740

gatttcgcct ccctgtgtga aaaaccggaa gtgaaagaat acattctgcg cgaactgacc     1800

aaaacgggca aagagaaaaa actgaaaggt ttcgaatttc tgaaagcagt tcatctggac     1860

ccggtgccgt ttgatatgga acgtgacctg ctgaccccga cgttcaagaa aaaacgtccg     1920

caactgctga aatactataa agatgtgatc gactcaatgt ataaaggcac gaaataa        1977


