                         SEQUENCE LISTING

<110>  Meng, Xiang-Jin 
       Subramaniam, Sakthivel 
       Virginia Tech Intellectual Properties
 
<120>  ANTIGEN TARGETING TO PROCINE LANGERIN

<130>  60762US01 (P-20935)

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  381
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region

<400>  1
atggcagctg cccaaagtat ccaagcacag atccagttgg tgcagtctgg acctgagctg       60

aagaagcctg gagagacagt caagatctcc tgcaaggctt ctggttatac cttcacagac      120

tattcaatgc actgggtgaa gcaggctcca ggaaagggtt taaagtggat gggctggata      180

aacactgaga ctggtgagcc aacatatgca gatgacttca agggacggtt tgccttctct      240

ttggaaacct ctgccagcac tgcctatttg cagatcaaca acctcaaaaa tgaggacacg      300

gctacatatt tctgtgctag agatatggac aactggggtc aaggaacctc agtcaccgtc      360

tccacagcca aaacgacacc c                                                381


<210>  2
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable sequence

<400>  2

Met Ala Ala Ala Gln Ser Ile Gln Ala Gln Ile Gln Leu Val Gln Ser 
1               5                   10                  15      


Gly Pro Glu Leu Lys Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys 
            20                  25                  30          


Ala Ser Gly Tyr Thr Phe Thr Asp Tyr Ser Met His Trp Val Lys Gln 
        35                  40                  45              


Ala Pro Gly Lys Gly Leu Lys Trp Met Gly Trp Ile Asn Thr Glu Thr 
    50                  55                  60                  


Gly Glu Pro Thr Tyr Ala Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser 
65                  70                  75                  80  


Leu Glu Thr Ser Ala Ser Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys 
                85                  90                  95      


Asn Glu Asp Thr Ala Thr Tyr Phe Cys Ala Arg Asp Met Asp Asn Trp 
            100                 105                 110         


Gly Gln Gly Thr Ser Val Thr Val Ser Thr Ala Lys Thr Thr Pro 
        115                 120                 125         


<210>  3
<211>  387
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  light chain variable sequence

<400>  3
atgttctgga ttcctgcttc cagcagtgat gttttgatga cccaaactcc actctcccta       60

cctgtcagtc ttggagatca agcctccatc tcttgcagat ctagtcagag cattgtacat      120

agtagtggaa acaccttttt agaatggtac ctgcagaaac caggccagtc tccaaagctc      180

ctgatctaca aagtttccaa ccgattttct ggggtcccag acaggttcag tggcactgga      240

tcagggacag atttcacact caagatcagc agagtggagg ctgaggatct gggagtttat      300

tactgctttc aaggttcaca tgttccgtac acgttcggag gggggaccaa gctggaaata      360

aaacgggctg atgctgcacc aactgta                                          387


<210>  4
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable sequence

<400>  4

Met Phe Trp Ile Pro Ala Ser Ser Ser Asp Val Leu Met Thr Gln Thr 
1               5                   10                  15      


Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys 
            20                  25                  30          


Arg Ser Ser Gln Ser Ile Val His Ser Ser Gly Asn Thr Phe Leu Glu 
        35                  40                  45              


Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys 
    50                  55                  60                  


Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Thr Gly 
65                  70                  75                  80  


Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp 
                85                  90                  95      


Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr Phe 
            100                 105                 110         


Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr 
        115                 120                 125             


Val 
    


<210>  5
<211>  1629
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3B3scFv-pIgG2Fc2

<400>  5
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

atatctattc ctgcttccag cagtgatgtt ttgatgaccc aaactccact ctccctacct      120

gtcagtcttg gagatcaagc ctccatctct tgcagatcta gtcagagcat tgtacatagt      180

agtggaaaca cctttttaga atggtacctg cagaaaccag gccagtctcc aaagctcctg      240

atctacaaag tttccaaccg attttctggg gtcccagaca ggttcagtgg cactggatca      300

gggacagatt tcacactcaa gatcagcaga gtggaggctg aggatctggg agtttattac      360

tgctttcaag gttcacatgt tccgtacacg ttcggagggg ggaccaagct ggaaataaaa      420

cgggctgatg ctgcaccaac tgtatccatc ttcccaccat ccagttcagg tggtggcggt      480

tcaggcggag gtggctctgg cggtggcgga tcgatggcag ctgcccaaag tatccaagca      540

cagatccagt tggtgcagtc tggacctgag ctgaagaagc ctggagagac agtcaagatc      600

tcctgcaagg cttctggtta taccttcaca gactattcaa tgcactgggt gaagcaggct      660

ccaggaaagg gtttaaagtg gatgggctgg ataaacactg agactggtga gccaacatat      720

gcagatgact tcaagggacg gtttgccttc tctttggaaa cctctgccag cactgcctat      780

ttgcagatca acaacctcaa aaatgaggac acggctacat atttctgtgc tagagatatg      840

gacaactggg gtcaaggaac ctcagtcacc gtctccacag ccaaaacgac acccccatct      900

gtctatccac tggcccctgg agccatggtt agatctggaa caaagaccaa accaccatgt      960

cccatatgcc cagcctgtga atcgccaggg ccctcggtct tcatcttccc tccaaaaccc     1020

aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcgtggtagt tgatgtgagc     1080

caggagaacc cggaggtcca gttctcctgg tacgtggacg gcgtagaggt gcacacggcc     1140

cagacgaggc caaaggagga gcagttcaac agcacctacc gcgtggtcag cgtcctgccc     1200

atccagcacc aggactggct gaacgggaag gagttcaagt gcaaggtcaa caacaaagac     1260

ctcccagccc ccatcacaag gatcatctcc aaggccaaag ggcagacccg ggagccgcag     1320

gtgtacaccc tgcccccacc caccgaggag ctgtccagga gcaaagtcac gctaacctgc     1380

ctggtcactg gcttctaccc acctgacatc gatgtcgagt ggcaaagaaa cggacagccg     1440

gagccagagg gcaattaccg caccaccccg ccccagcagg acgtggacgg gacctacttc     1500

ctgtacagca agttctcggt ggacaaggcc agctggcagg gtggaggcat attccagtgt     1560

gcggtgatgc acgaggctct gcacaaccac tacacccaga agtctatctc caagactccg     1620

ggtaaatga                                                             1629


<210>  6
<211>  543
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3B3scFv- pIgG2Fc2

<400>  6

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Ile Ser Ile Pro Ala Ser Ser Ser Asp Val Leu Met 
            20                  25                  30          


Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser 
        35                  40                  45              


Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser Ser Gly Asn Thr 
    50                  55                  60                  


Phe Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu 
65                  70                  75                  80  


Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser 
                85                  90                  95      


Gly Thr Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu 
            100                 105                 110         


Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro 
        115                 120                 125             


Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala 
    130                 135                 140                 


Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Ala Ala Ala Gln 
                165                 170                 175     


Ser Ile Gln Ala Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys 
            180                 185                 190         


Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr 
        195                 200                 205             


Phe Thr Asp Tyr Ser Met His Trp Val Lys Gln Ala Pro Gly Lys Gly 
    210                 215                 220                 


Leu Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Thr Tyr 
225                 230                 235                 240 


Ala Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala 
                245                 250                 255     


Ser Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala 
            260                 265                 270         


Thr Tyr Phe Cys Ala Arg Asp Met Asp Asn Trp Gly Gln Gly Thr Ser 
        275                 280                 285             


Val Thr Val Ser Thr Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu 
    290                 295                 300                 


Ala Pro Gly Ala Met Val Arg Ser Gly Thr Lys Thr Lys Pro Pro Cys 
305                 310                 315                 320 


Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe 
                325                 330                 335     


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val 
            340                 345                 350         


Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe 
        355                 360                 365             


Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln Thr Arg Pro 
    370                 375                 380                 


Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro 
385                 390                 395                 400 


Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val 
                405                 410                 415     


Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala 
            420                 425                 430         


Lys Gly Gln Thr Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Pro Thr 
        435                 440                 445             


Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Thr Gly 
    450                 455                 460                 


Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln Pro 
465                 470                 475                 480 


Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val Asp 
                485                 490                 495     


Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser Trp 
            500                 505                 510         


Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu His 
        515                 520                 525             


Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys Leu 
    530                 535                 540             


<210>  7
<211>  3792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3B3scFv-pIgG2Fc2-PEDV S Ag chimera

<400>  7
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

atatctattc ctgcttccag cagtgatgtt ttgatgaccc aaactccact ctccctacct      120

gtcagtcttg gagatcaagc ctccatctct tgcagatcta gtcagagcat tgtacatagt      180

agtggaaaca cctttttaga atggtacctg cagaaaccag gccagtctcc aaagctcctg      240

atctacaaag tttccaaccg attttctggg gtcccagaca ggttcagtgg cactggatca      300

gggacagatt tcacactcaa gatcagcaga gtggaggctg aggatctggg agtttattac      360

tgctttcaag gttcacatgt tccgtacacg ttcggagggg ggaccaagct ggaaataaaa      420

cgggctgatg ctgcaccaac tgtatccatc ttcccaccat ccagttcagg tggtggcggt      480

tcaggcggag gtggctctgg cggtggcgga tcgatggcag ctgcccaaag tatccaagca      540

cagatccagt tggtgcagtc tggacctgag ctgaagaagc ctggagagac agtcaagatc      600

tcctgcaagg cttctggtta taccttcaca gactattcaa tgcactgggt gaagcaggct      660

ccaggaaagg gtttaaagtg gatgggctgg ataaacactg agactggtga gccaacatat      720

gcagatgact tcaagggacg gtttgccttc tctttggaaa cctctgccag cactgcctat      780

ttgcagatca acaacctcaa aaatgaggac acggctacat atttctgtgc tagagatatg      840

gacaactggg gtcaaggaac ctcagtcacc gtctccacag ccaaaacgac acccccatct      900

gtctatccac tggcccctgg agccatggtt agatctggaa caaagaccaa accaccatgt      960

cccatatgcc cagcctgtga atcgccaggg ccctcggtct tcatcttccc tccaaaaccc     1020

aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcgtggtagt tgatgtgagc     1080

caggagaacc cggaggtcca gttctcctgg tacgtggacg gcgtagaggt gcacacggcc     1140

cagacgaggc caaaggagga gcagttcaac agcacctacc gcgtggtcag cgtcctgccc     1200

atccagcacc aggactggct gaacgggaag gagttcaagt gcaaggtcaa caacaaagac     1260

ctcccagccc ccatcacaag gatcatctcc aaggccaaag ggcagacccg ggagccgcag     1320

gtgtacaccc tgcccccacc caccgaggag ctgtccagga gcaaagtcac gctaacctgc     1380

ctggtcactg gcttctaccc acctgacatc gatgtcgagt ggcaaagaaa cggacagccg     1440

gagccagagg gcaattaccg caccaccccg ccccagcagg acgtggacgg gacctacttc     1500

ctgtacagca agttctcggt ggacaaggcc agctggcagg gtggaggcat attccagtgt     1560

gcggtgatgc acgaggctct gcacaaccac tacacccaga agtctatctc caagactccg     1620

ggtaaatcag gtctcgagca agatgtcacc aggtgctcag ctaacactaa ttttaggcgg     1680

ttcttttcaa aatttaatgt tcaggcgcct gcagttgttg tactgggcgg ttatctacct     1740

attggtgaaa accagggtgt caattcaact tggtactgtg ctggccaaca tccaactgct     1800

agtggcgttc atggtatctt tgttagccat attagaggtg gtcatggctt tgagattggc     1860

atttcgcaag agccttttga ccctagtggt taccagcttt atttacataa ggctactaac     1920

ggtaacacta atgctactgc gcgactgcgc atttgccagt ttcctagcat taaaacattg     1980

ggccccactg ctaataatga tgttacaaca ggtcgtaatt gcctatttaa caaagccatc     2040

ccagctcata tgagtgaaca tagtgttgtc ggcataacat gggataatga tcgtgtcact     2100

gtcttttctg acaagatcta ttatttttat tttaaaaatg attggtcccg tgttgcgaca     2160

aagtgttaca acagtggagg ttgtgctatg caatatgttt acgaacccac ctattacatg     2220

cttaatgtta ctagtgctgg tgaggatggt attttttatc aaccctgtac agctaattgc     2280

attggttatg ctgccaatgt atttgctact gagcccaatg gccacatacc agaaggtttt     2340

agttttaata attggtttct tttgtccaat gattccactt tggtgcatgg taaggtggtt     2400

tccaaccaac cattgttggt caattgtctt ttggccattc ctaagattta tggactaggc     2460

caatttttct cctttaatca aacgatcgat ggtgtttgta atggagctgc tgtgcagcgt     2520

gcaccagagg ctctgaggtt taatattaat gacacctctg tcattcttgc tgaaggctca     2580

attgtacttc atactgcttt aggaacaaat ttttcttttg tttgcagtaa ttcctcaaat     2640

cctcacttag ccaccttcgc catacctttg ggtgctaccc aagtacccta ttattgtttt     2700

cttaaagtgg atacttacaa ctccactgtt tataaattct tggctgtttt acctccaacc     2760

gtcagggaaa ttgtcatcac caagtatggt gatgtttatg tcaatgggtt tggatacttg     2820

catctcggtt tgttggatgc tgtcacaatt aatttcactg gtcatggcac tgacgatgat     2880

gtttctggtt tttggaccat agcatcgact aattttgttg atgcactcat cgaagttcaa     2940

ggaaccgcca ttcagcgtat tctttattgt gatgatcctg ttagccaact caagtgttct     3000

caggttgctt ttgaccttga cgatggtttt taccatattt cttctagaaa ccttctgagt     3060

catgaacagc caatttcttt tgttactctg ccatcattta atgatcattc ttttgttaac     3120

attactgtat ctgcttcctt tggtggtcat agtggtgcca accttattgc atctgacact     3180

actatcaatg ggtttagttc tttctgtgtt gacactagac aatttaccat ttcactgttt     3240

tataacgtta caaacagtta tggttatgtg tctaaatcac aggacagtaa ttgccctttc     3300

accttgcaat ctgttaatga ttacctgtct tttagcaaat tttgtgtttc caccagcctt     3360

ttggctagtg cctgtaccat agatcttttt ggttaccctg agtttggtag tggtgttaag     3420

tttacgtccc tttactttca attcacaaag ggtgagttga ttactggcac gcctaaacca     3480

cttgaaggtg tcacggacgt ttcttttatg actctggatg tgtgtaccaa gtatactatc     3540

tatggcttta aaggtgaggg tatcattacc cttacaaatt ctagcttttt ggcaggtgtt     3600

tattacacat ctgattctgg acagttgtta gcctttaaga atgtcactag tggtgctgtt     3660

tattctgtta cgccatgttc tttttcagag caggctgcat atgttgatga tgatatagtg     3720

ggtgttattt ctagtttgtc tagctccact tttaacagta ctagggagtt gcctggtttc     3780

ttctaccatt ga                                                         3792


