                         SEQUENCE LISTING

<110>  Northwestern University
       Jewett, Michael Christopher
       Stark, Jessica Carol
       DeLisa, Matthew P.
       Jaroentomeechai, Thapakorn
 
<120>  CELL-FREE GLYCOPROTEIN SYNTHESIS (CFGPS) IN PROKARYOTIC CELL 
       LYSATES ENRICHED WITH COMPONENTS FOR GLYCOSYLATION

<130>  5369-00333

<150>  US 62/273,124
<151>  2015-12-30

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  1122
<212>  DNA
<213>  Escherichia coli

<400>  1
atgtcaaaag tcgctctcat caccggtgta accggacaag acggttctta cctggcagag       60

tttctgctgg aaaaaggtta cgaggtgcat ggtattaagc gtcgcgcatc gtcattcaac      120

accgagcgcg tggatcacat ttatcaggat ccgcacacct gcaacccgaa attccatctg      180

cattatggcg acctgagtga tacctctaac ctgacgcgca ttttgcgtga agtacagccg      240

gatgaagtgt acaacctggg cgcaatgagc cacgttgcgg tctcttttga gtcaccagaa      300

tataccgctg acgtcgacgc gatgggtacg ctgcgcctgc tggaggcgat ccgcttcctc      360

ggtctggaaa agaaaactcg tttctatcag gcttccacct ctgaactgta tggtctggtg      420

caggaaattc cgcagaaaga gaccacgccg ttctacccgc gatctccgta tgcggtcgcc      480

aaactgtacg cctactggat caccgttaac taccgtgaat cctacggcat gtacgcctgt      540

aacggaattc tcttcaacca tgaatccccg cgccgcggcg aaaccttcgt tacccgcaaa      600

atcacccgcg caatcgccaa catcgcccag gggctggagt cgtgcctgta cctcggcaat      660

atggattccc tgcgtgactg gggccacgcc aaagactacg taaaaatgca gtggatgatg      720

ctgcagcagg aacagccgga agatttcgtt atcgcgaccg gcgttcagta ctccgtgcgt      780

cagttcgtgg aaatggcggc agcacagctg ggcatcaaac tgcgctttga aggcacgggc      840

gttgaagaga agggcattgt ggtttccgtc accgggcatg acgcgccggg cgttaaaccg      900

ggtgatgtga ttatcgctgt tgacccgcgt tacttccgtc cggctgaagt tgaaacgctg      960

ctcggcgacc cgaccaaagc gcacgaaaaa ctgggctgga aaccggaaat caccctcaga     1020

gagatggtgt ctgaaatggt ggctaatgac ctcgaagcgg cgaaaaaaca ctctctgctg     1080

aaatctcacg gctacgacgt ggcgatcgcg ctggagtcat aa                        1122


<210>  2
<211>  373
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Ser Lys Val Ala Leu Ile Thr Gly Val Thr Gly Gln Asp Gly Ser 
1               5                   10                  15      


Tyr Leu Ala Glu Phe Leu Leu Glu Lys Gly Tyr Glu Val His Gly Ile 
            20                  25                  30          


Lys Arg Arg Ala Ser Ser Phe Asn Thr Glu Arg Val Asp His Ile Tyr 
        35                  40                  45              


Gln Asp Pro His Thr Cys Asn Pro Lys Phe His Leu His Tyr Gly Asp 
    50                  55                  60                  


Leu Ser Asp Thr Ser Asn Leu Thr Arg Ile Leu Arg Glu Val Gln Pro 
65                  70                  75                  80  


Asp Glu Val Tyr Asn Leu Gly Ala Met Ser His Val Ala Val Ser Phe 
                85                  90                  95      


Glu Ser Pro Glu Tyr Thr Ala Asp Val Asp Ala Met Gly Thr Leu Arg 
            100                 105                 110         


Leu Leu Glu Ala Ile Arg Phe Leu Gly Leu Glu Lys Lys Thr Arg Phe 
        115                 120                 125             


Tyr Gln Ala Ser Thr Ser Glu Leu Tyr Gly Leu Val Gln Glu Ile Pro 
    130                 135                 140                 


Gln Lys Glu Thr Thr Pro Phe Tyr Pro Arg Ser Pro Tyr Ala Val Ala 
145                 150                 155                 160 


Lys Leu Tyr Ala Tyr Trp Ile Thr Val Asn Tyr Arg Glu Ser Tyr Gly 
                165                 170                 175     


Met Tyr Ala Cys Asn Gly Ile Leu Phe Asn His Glu Ser Pro Arg Arg 
            180                 185                 190         


Gly Glu Thr Phe Val Thr Arg Lys Ile Thr Arg Ala Ile Ala Asn Ile 
        195                 200                 205             


Ala Gln Gly Leu Glu Ser Cys Leu Tyr Leu Gly Asn Met Asp Ser Leu 
    210                 215                 220                 


Arg Asp Trp Gly His Ala Lys Asp Tyr Val Lys Met Gln Trp Met Met 
225                 230                 235                 240 


Leu Gln Gln Glu Gln Pro Glu Asp Phe Val Ile Ala Thr Gly Val Gln 
                245                 250                 255     


Tyr Ser Val Arg Gln Phe Val Glu Met Ala Ala Ala Gln Leu Gly Ile 
            260                 265                 270         


Lys Leu Arg Phe Glu Gly Thr Gly Val Glu Glu Lys Gly Ile Val Val 
        275                 280                 285             


Ser Val Thr Gly His Asp Ala Pro Gly Val Lys Pro Gly Asp Val Ile 
    290                 295                 300                 


Ile Ala Val Asp Pro Arg Tyr Phe Arg Pro Ala Glu Val Glu Thr Leu 
305                 310                 315                 320 


Leu Gly Asp Pro Thr Lys Ala His Glu Lys Leu Gly Trp Lys Pro Glu 
                325                 330                 335     


Ile Thr Leu Arg Glu Met Val Ser Glu Met Val Ala Asn Asp Leu Glu 
            340                 345                 350         


Ala Ala Lys Lys His Ser Leu Leu Lys Ser His Gly Tyr Asp Val Ala 
        355                 360                 365             


Ile Ala Leu Glu Ser 
    370             


<210>  3
<211>  1260
<212>  DNA
<213>  Escherichia coli

<400>  3
atgctaacat cctttaaact tcattcattg aaaccttaca ctctgaaatc atcaatgatt       60

ttagagataa taacttatat attatgtttt ttttcaatga taattgcatt cgtcgataat      120

actttcagca taaaaatata taatatcact gctatagttt gcttattgtc actaatttta      180

cgtggcagac aagaaaatta taatataaaa aaccttattc ttcccctttc tatattttta      240

ataggcttgc ttgatttaat ttggtattct gcgtttaaag tagataattc gccatttcgt      300

gctacttacc atagttattt aaatactgcc aaaatattta tatttggttc ttttattgtt      360

ttcttgacac taactagcca gctaaaatca aaaaaagaga gtgtattata cactttgtat      420

tctctgtcat ttctaattgc tggatatgca atgtatatta atagcattca tgaaaatgac      480

cgcatttctt ttggtgtagg aacggcaaca ggagcagcat attcaacaat gctaataggg      540

atagttagtg gcgttgcgat tctttatact aagaaaaatc atcctttttt atttttatta      600

aatagttgcg cggtacttta tgttctggcg ctaacacaaa ccagagcaac cctactcctg      660

ttccctataa tttgtgttgc tgcattaata gcttattata ataaatcacc caagaaattc      720

acttcctcta ttgttctact aattgctata ttagctagca ttgttattat atttaataaa      780

ccaatacaga atcgctataa tgaagcatta aatgacttaa acagttatac caatgctaat      840

agtgttactt ccctaggtgc aagactggca atgtacgaaa ttggtttaaa tatattcata      900

aagtcacctt tttcatttag atcagcagag tcacgcgctg aaagtatgaa tttgttagtt      960

gcagaacaca ataggctaag aggggcattg gagttttcta acgtacatct acataatgag     1020

ataattgaag cagggtcact gaaaggtctg atgggaattt tttccacact tttcctctat     1080

ttttcactat tttatatagc atataaaaaa cgagctttgg gtttgttgat attaacgctt     1140

ggcattgtgg ggattggact cagtgatgtg atcatatggg cacgcagcat tccaattatc     1200

attatatccg ctatagtcct cttactcgtc attaataatc gtaacaatac aattaattaa     1260


<210>  4
<211>  419
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Leu Thr Ser Phe Lys Leu His Ser Leu Lys Pro Tyr Thr Leu Lys 
1               5                   10                  15      


Ser Ser Met Ile Leu Glu Ile Ile Thr Tyr Ile Leu Cys Phe Phe Ser 
            20                  25                  30          


Met Ile Ile Ala Phe Val Asp Asn Thr Phe Ser Ile Lys Ile Tyr Asn 
        35                  40                  45              


Ile Thr Ala Ile Val Cys Leu Leu Ser Leu Ile Leu Arg Gly Arg Gln 
    50                  55                  60                  


Glu Asn Tyr Asn Ile Lys Asn Leu Ile Leu Pro Leu Ser Ile Phe Leu 
65                  70                  75                  80  


Ile Gly Leu Leu Asp Leu Ile Trp Tyr Ser Ala Phe Lys Val Asp Asn 
                85                  90                  95      


Ser Pro Phe Arg Ala Thr Tyr His Ser Tyr Leu Asn Thr Ala Lys Ile 
            100                 105                 110         


Phe Ile Phe Gly Ser Phe Ile Val Phe Leu Thr Leu Thr Ser Gln Leu 
        115                 120                 125             


Lys Ser Lys Lys Glu Ser Val Leu Tyr Thr Leu Tyr Ser Leu Ser Phe 
    130                 135                 140                 


Leu Ile Ala Gly Tyr Ala Met Tyr Ile Asn Ser Ile His Glu Asn Asp 
145                 150                 155                 160 


Arg Ile Ser Phe Gly Val Gly Thr Ala Thr Gly Ala Ala Tyr Ser Thr 
                165                 170                 175     


Met Leu Ile Gly Ile Val Ser Gly Val Ala Ile Leu Tyr Thr Lys Lys 
            180                 185                 190         


Asn His Pro Phe Leu Phe Leu Leu Asn Ser Cys Ala Val Leu Tyr Val 
        195                 200                 205             


Leu Ala Leu Thr Gln Thr Arg Ala Thr Leu Leu Leu Phe Pro Ile Ile 
    210                 215                 220                 


Cys Val Ala Ala Leu Ile Ala Tyr Tyr Asn Lys Ser Pro Lys Lys Phe 
225                 230                 235                 240 


Thr Ser Ser Ile Val Leu Leu Ile Ala Ile Leu Ala Ser Ile Val Ile 
                245                 250                 255     


Ile Phe Asn Lys Pro Ile Gln Asn Arg Tyr Asn Glu Ala Leu Asn Asp 
            260                 265                 270         


Leu Asn Ser Tyr Thr Asn Ala Asn Ser Val Thr Ser Leu Gly Ala Arg 
        275                 280                 285             


Leu Ala Met Tyr Glu Ile Gly Leu Asn Ile Phe Ile Lys Ser Pro Phe 
    290                 295                 300                 


Ser Phe Arg Ser Ala Glu Ser Arg Ala Glu Ser Met Asn Leu Leu Val 
305                 310                 315                 320 


Ala Glu His Asn Arg Leu Arg Gly Ala Leu Glu Phe Ser Asn Val His 
                325                 330                 335     


Leu His Asn Glu Ile Ile Glu Ala Gly Ser Leu Lys Gly Leu Met Gly 
            340                 345                 350         


Ile Phe Ser Thr Leu Phe Leu Tyr Phe Ser Leu Phe Tyr Ile Ala Tyr 
        355                 360                 365             


Lys Lys Arg Ala Leu Gly Leu Leu Ile Leu Thr Leu Gly Ile Val Gly 
    370                 375                 380                 


Ile Gly Leu Ser Asp Val Ile Ile Trp Ala Arg Ser Ile Pro Ile Ile 
385                 390                 395                 400 


Ile Ile Ser Ala Ile Val Leu Leu Leu Val Ile Asn Asn Arg Asn Asn 
                405                 410                 415     


Thr Ile Asn 
            


