                         SEQUENCE LISTING

<110>  LanzaTech New Zealand Limited
 
<120>  MICROORGANISM WITH MODIFIED HYDROGENASE ACTIVITY

<130>  LT120WO1

<150>  62/271,466
<151>  2015-12-28

<160>  71    

<170>  PatentIn version 3.5

<210>  1
<211>  161
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_2794, NADH-quinone oxidoreductase, E subunit (EC:1.6.99.5)

<400>  1

Met Leu Thr Lys Gln Gln Asn Glu Asp Leu Ser Gly Gln Asp Val Ile 
1               5                   10                  15      


Glu Lys Tyr Pro Lys Glu Gln Arg Phe Thr Leu Ala Ile Leu Gln Asp 
            20                  25                  30          


Ile Gln Arg Lys Tyr Lys Tyr Ile Pro Arg Glu Ala Leu Glu Asn Leu 
        35                  40                  45              


Ala Lys Tyr Leu Asp Thr Pro Val Ser Arg Leu Tyr Gly Met Ala Thr 
    50                  55                  60                  


Phe Tyr Lys Ala Leu Ser Leu Thr Pro Lys Gly Glu Asn Ile Ile Thr 
65                  70                  75                  80  


Val Cys Asp Gly Thr Ala Cys His Val Ala Gly Ser Met Val Val Met 
                85                  90                  95      


Asp Glu Leu Glu Lys Ala Ile Gly Ile Lys Pro Gly Glu Thr Thr Glu 
            100                 105                 110         


Asp Leu Lys Phe Ser Ile Asn Thr Val Asn Cys Ile Gly Cys Cys Ala 
        115                 120                 125             


Ile Ala Pro Val Met Met Ile Asn Asp Lys Tyr Tyr Gly Asn Leu Thr 
    130                 135                 140                 


Pro Lys Leu Val Glu Glu Ile Leu Ser Glu Tyr Arg Ser Glu Ser Asp 
145                 150                 155                 160 


Glu 
    


<210>  2
<211>  486
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_2794, NADH-quinone oxidoreductase, E subunit (EC:1.6.99.5)

<400>  2
atgttaacta aacagcaaaa tgaagacctg tctggacaag atgtaattga aaaatatcct       60

aaagagcaga gatttactct tgctatacta caggatatac agagaaagta caaatatata      120

ccaagagaag cactggagaa tttagctaag tatttggaca cgcctgtaag tagactgtat      180

ggtatggcta ctttttataa ggcattgagc cttactccaa aaggggaaaa cataataact      240

gtatgtgatg gaaccgcttg ccatgttgct ggttctatgg ttgtaatgga tgaacttgaa      300

aaggcaatag gaattaaacc aggtgaaact acagaggatc tcaaattttc aataaataca      360

gttaactgta taggatgctg tgcaatagct cctgtcatga tgataaatga caaatattat      420

ggaaatttaa cacctaaact ggttgaagaa attcttagtg agtataggag tgagagtgat      480

gagtga                                                                 486


<210>  3
<211>  599
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_2795, NADH dehydrogenase (quinone) (EC:1.6.99.5)

<400>  3

Met Ser Asp Lys Lys Thr Val Asn Ile Cys Cys Gly Thr Gly Cys Leu 
1               5                   10                  15      


Ala Lys Gly Ser Met Glu Val Tyr Glu Glu Met Lys Ala Gln Ile Ala 
            20                  25                  30          


Lys Leu Gly Ala Asn Ala Glu Val Asn Val Lys Leu Lys Ala Thr Gly 
        35                  40                  45              


Cys Asp Gly Leu Cys Glu Lys Gly Pro Val Leu Lys Ile Tyr Pro Asp 
    50                  55                  60                  


Asp Ile Ala Tyr Phe Lys Val Lys Val Glu Asp Val Glu Asp Val Val 
65                  70                  75                  80  


Lys Lys Thr Leu Met Asn Gly Glu Ile Ile Glu Lys Leu Leu Tyr Phe 
                85                  90                  95      


Glu Thr Ala Thr Lys Gln Arg Leu Arg Asn His Lys Glu Ser Glu Phe 
            100                 105                 110         


Cys Lys Arg Gln Tyr Lys Ile Ala Leu Arg Asn Val Gly Glu Ile Asp 
        115                 120                 125             


Pro Ile Ser Leu Glu Asp Tyr Val Glu Arg Gly Gly Tyr Lys Ala Leu 
    130                 135                 140                 


Lys Lys Ala Ile Ser Ser Met Lys Pro Glu Asp Val Leu Glu Glu Ile 
145                 150                 155                 160 


Thr Lys Ser Gly Leu Arg Gly Arg Gly Gly Ala Gly Phe Pro Thr Gly 
                165                 170                 175     


Arg Lys Trp Lys Thr Ala Ala Asp Ile Asp Thr Ser Pro Ile Tyr Val 
            180                 185                 190         


Val Cys Asn Gly Asp Glu Gly Asp Pro Gly Ala Phe Met Asp Arg Ser 
        195                 200                 205             


Ile Met Glu Gly Asp Pro Asn Ser Val Ile Glu Gly Met Thr Leu Cys 
    210                 215                 220                 


Ala Tyr Ala Val Gly Gly Thr Asn Gly Phe Ala Tyr Ile Arg Asp Glu 
225                 230                 235                 240 


Tyr Gly Leu Ala Val Glu Asn Met Gln Lys Ala Ile Asn Lys Ala Lys 
                245                 250                 255     


Asp Glu Asn Leu Leu Gly Asn Asn Ile Leu Gly Thr Asp Phe Ser Phe 
            260                 265                 270         


Asp Ile Gln Ile Val Arg Gly Gly Gly Ala Phe Val Cys Gly Glu Ser 
        275                 280                 285             


Thr Ala Leu Met Ser Ser Ile Glu Gly Met Val Gly Glu Pro Arg Ala 
    290                 295                 300                 


Lys Tyr Ile His Thr Thr Glu Lys Gly Leu Trp Gly Gln Pro Thr Val 
305                 310                 315                 320 


Leu Asn Asn Val Glu Thr Trp Ala Asn Val Pro Ile Ile Ile Glu Lys 
                325                 330                 335     


Gly Gly Asp Trp Tyr His Ala Ile Gly Thr Met Glu Lys Ser Lys Gly 
            340                 345                 350         


Thr Lys Val Phe Ser Leu Val Gly Lys Val Lys Asn Thr Gly Leu Val 
        355                 360                 365             


Glu Val Pro Met Gly Thr Thr Leu Arg Glu Ile Ile Tyr Asp Ile Gly 
    370                 375                 380                 


Gly Gly Val Leu Asn Asp Arg Lys Phe Lys Ala Val Gln Ile Gly Gly 
385                 390                 395                 400 


Pro Ser Gly Gly Cys Leu Pro Ala Glu Tyr Leu Asp Leu Pro Val Asp 
                405                 410                 415     


Tyr Asp Thr Leu Val Lys Ala Asp Ser Met Met Gly Ser Gly Gly Met 
            420                 425                 430         


Ile Val Met Asp Asp Arg Thr Cys Met Val Asp Val Thr Arg Tyr Tyr 
        435                 440                 445             


Leu Ser Phe Leu Ala Glu Glu Ser Cys Gly Lys Cys Val Pro Cys Arg 
    450                 455                 460                 


Glu Gly Val Lys Arg Met Leu Glu Ile Leu Thr Asp Ile Cys Asn Gly 
465                 470                 475                 480 


Asp Gly Lys Glu Gly Asp Ile Glu Glu Leu Leu Glu Ile Cys Ser Met 
                485                 490                 495     


Thr Ser Lys Ala Ser Leu Cys Ser Leu Gly Lys Ser Ala Pro Asn Pro 
            500                 505                 510         


Val Ile Ala Ser Ile Arg Tyr Phe Arg Asp Glu Phe Glu Glu His Ile 
        515                 520                 525             


Lys Asn Lys Arg Cys Arg Ala Gly Val Cys Lys Lys Leu Thr Thr Phe 
    530                 535                 540                 


Gly Ile Asp Glu Asp Lys Cys Lys Gly Cys Asp Met Cys Lys Lys Asn 
545                 550                 555                 560 


Cys Pro Ala Asp Cys Ile Thr Gly Glu Ile Lys Lys Pro His Thr Ile 
                565                 570                 575     


Asp Ala Asp Lys Cys Leu Arg Cys Gly Asn Cys Met Asn Ile Cys Lys 
            580                 585                 590         


Phe Asp Ala Val Lys Val Leu 
        595                 


<210>  4
<211>  1800
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_2795, NADH dehydrogenase (quinone) (EC:1.6.99.5)

<400>  4
atgagtgata aaaaaactgt caatatatgt tgtgggacag gttgtttagc taaaggcagt       60

atggaagtat atgaagaaat gaaggcacaa atagctaaat taggggcaaa tgcagaagta      120

aatgttaaat taaaagcaac aggttgcgat ggattgtgtg agaaaggtcc tgtactgaaa      180

atatatccag atgacattgc atattttaaa gttaaagtag aagatgtaga agacgtagta      240

aaaaagacat tgatgaatgg ggaaataatt gaaaaattat tatattttga gactgctaca      300

aaacagagat taagaaatca taaagaaagt gaattttgta aaagacaata caaaattgct      360

ctcagaaatg ttggtgaaat agatccaata agtttggaag attatgttga aagaggcgga      420

tacaaagctc ttaaaaaagc aataagcagc atgaaacctg aagatgtgct tgaagaaata      480

acaaaatcag gtcttagagg aagaggtgga gcaggattcc caacaggacg taaatggaaa      540

actgctgcag atattgatac atcacctata tatgtagtat gtaatggtga tgaaggagat      600

cctggagcat ttatggatag aagtataatg gagggagatc ctaacagtgt tatagaaggt      660

atgacattat gtgcttatgc agtaggaggt acaaatggat ttgcttatat aagagatgaa      720

tatggacttg ctgtagaaaa tatgcagaaa gctattaata aagcaaaaga tgaaaattta      780

ttaggtaata atatattagg aactgatttt tccttcgata tacagatagt aagaggtgga      840

ggagcttttg tatgtggtga atctactgca cttatgtcgt ctatagaagg tatggtaggt      900

gaacctagag ctaaatatat acacactaca gaaaaaggat tgtggggaca acctacagtt      960

ttaaataatg tagaaacttg ggccaatgta cctataataa ttgaaaaagg cggagattgg     1020

tatcatgcta taggaactat ggagaagagt aagggaacaa aggtattctc attagttgga     1080

aaagttaaga atactggact tgtagaagta cctatgggaa ctactcttag agaaataata     1140

tatgatattg gcggtggagt attaaatgat agaaagttta aggcagttca aataggtgga     1200

ccttcaggtg gatgtttacc agctgaatat ttagatttgc cagtagatta tgatactttg     1260

gttaaagcag attccatgat gggttcaggc ggaatgatcg taatggatga tagaacctgt     1320

atggtagatg taactagata ttacctgagc ttcttggctg aagaatcttg tggaaagtgt     1380

gtaccttgta gagaaggcgt aaagaggatg cttgaaatac tcactgacat atgcaatggt     1440

gatggaaaag aaggagacat agaagagctt ctcgaaatat gttccatgac aagcaaggca     1500

tctctgtgca gtcttggtaa gagtgctcca aatccagtaa ttgcttctat aagatatttt     1560

agagatgaat ttgaagagca tataaagaat aagagatgta gagcaggagt ttgtaagaaa     1620

cttactacat ttggtataga cgaggataaa tgtaagggat gcgatatgtg taaaaagaat     1680

tgtccagctg attgtataac aggggaaatt aagaaaccac atacaataga tgctgataag     1740

tgcttgagat gcggtaactg catgaacatc tgtaagtttg atgctgttaa ggttctatag     1800


<210>  5
<211>  251
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_2796, 4Fe-4S ferredoxin, iron-sulpur binding 
       domain-containing protein

<400>  5

Met Lys Ile Thr Ile Asp Gly Lys Ala Cys Glu Ala Glu Lys Gly Glu 
1               5                   10                  15      


Phe Ile Leu Gln Ile Ala Arg Arg Asn Asn Ile Tyr Ile Pro Thr Leu 
            20                  25                  30          


Cys His Ser Asp Ala Leu Pro Gly Leu Ala Ser Cys Arg Leu Cys Ile 
        35                  40                  45              


Val Lys Val Val Asp Arg Gly Arg Ala Lys Ile Val Thr Ser Cys Ile 
    50                  55                  60                  


Phe Pro Val Ser Lys Glu Val Glu Val Ile Thr Asn Asp Asp Glu Ile 
65                  70                  75                  80  


Lys Arg Met Arg Lys Asn Ile Val Met Leu Leu Lys Val Arg Cys Pro 
                85                  90                  95      


Glu Asn Lys Glu Val Asn Glu Leu Ala Lys Ala Phe Gly Val Glu Glu 
            100                 105                 110         


Lys Arg Val Lys Arg Phe Lys Leu Asp Pro Glu Gln Asn Cys Val Leu 
        115                 120                 125             


Cys Gly Leu Cys Ala Lys Ala Cys Lys Glu Leu Gly Thr Gly Ala Ile 
    130                 135                 140                 


Ser Thr Val Asn Arg Gly Met Tyr Lys Glu Val Ala Thr Pro Tyr His 
145                 150                 155                 160 


Glu Ser Ser Pro Glu Cys Ile Gly Cys Ala Ser Cys Ala Asn Val Cys 
                165                 170                 175     


Pro Thr Asn Ala Ile Lys Val Val Asp Lys Asp Gly Glu Arg Glu Ile 
            180                 185                 190         


Trp Gly Lys Lys Phe Lys Met Val Lys Cys Asp Leu Cys Gly Glu Tyr 
        195                 200                 205             


Phe Ala Thr Glu Glu His Val Lys Tyr Ala Tyr Asn Arg Leu Gly Lys 
    210                 215                 220                 


Glu Gln Pro Glu Lys Leu Met Cys Ser Ser Cys Lys Lys Lys Val Thr 
225                 230                 235                 240 


Ala Lys Asp Val Lys Asn Ile Phe Glu Asn Val 
                245                 250     


<210>  6
<211>  756
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_2796, 4Fe-4S ferredoxin, iron-sulpur binding 
       domain-containing protein

<400>  6
atgaaaatta caatagatgg aaaagcttgt gaagctgaaa aaggagaatt catattacaa       60

atagcaagaa gaaataacat atatatacct acattatgtc acagcgatgc attgcctggg      120

cttgctagct gtagactatg tatagttaaa gtagtagata ggggacgtgc aaagatagta      180

acttcctgta tattccctgt aagtaaggaa gtagaagtta taactaatga cgatgaaata      240

aagagaatga gaaaaaacat agttatgctt ttaaaagtaa gatgccctga aaataaagag      300

gtaaatgaat tagctaaagc ctttggagta gaggaaaaga gagtaaagag gttcaaattg      360

gatccagaac aaaattgtgt tttgtgcgga ctttgtgcaa aagcttgcaa ggaattaggt      420

actggagcaa tttcaacagt taataggggt atgtataaag aagtagcaac tccatatcac      480

gaatcttcac cggaatgtat aggatgtgct tcctgtgcaa atgtttgtcc aactaatgca      540

ataaaagttg tggataaaga tggagaaaga gaaatatggg gcaaaaaatt caagatggtt      600

aagtgtgatt tgtgcggaga atattttgct acagaagaac atgtaaaata tgcttacaat      660

aggcttggaa aagagcagcc agaaaaactt atgtgtagca gctgcaagaa gaaagttaca      720

gccaaagatg tcaaaaatat ttttgagaac gtgtga                                756


<210>  7
<211>  184
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_2797, 4Fe-4S ferredoxin, iron-sulpur binding 
       domain-containing protein, WP_013237376.1

<400>  7

Met Lys Pro Glu Phe Asn Ser Phe Val Ile Ala Asp Pro Asp Lys Cys 
1               5                   10                  15      


Ile Gly Cys Arg Ser Cys Glu Ile Ala Cys Ala Ala Lys His Arg Glu 
            20                  25                  30          


Asp Thr Gln Gly Lys Thr Ile Gly Thr Met Asn Asn Lys Val Thr Pro 
        35                  40                  45              


Arg Leu Phe Phe Val Lys Asn Lys Gly Asn Val Met Pro Val Gln Cys 
    50                  55                  60                  


Arg His Cys Glu Asp Ala Pro Cys Leu Asn Ala Cys Pro Val Asn Ala 
65                  70                  75                  80  


Ile Val Glu Lys Asp Gly Ser Ile Ile Ile Asn Glu Ser Ala Cys Ile 
                85                  90                  95      


Gly Cys Gln Thr Cys Thr Ile Val Cys Pro Val Gly Ala Val Ser Leu 
            100                 105                 110         


Leu Pro Arg Thr Gln Gly Lys Val Val Thr Gly Gly Ile Gln Val Lys 
        115                 120                 125             


Val Arg Ala Ala Ala Tyr Lys Cys Asp Leu Cys Lys Glu Glu Gly Gly 
    130                 135                 140                 


Glu Pro Ala Cys Val Lys Glu Cys Pro Lys Glu Ala Leu Arg Leu Val 
145                 150                 155                 160 


Asp Pro Arg Glu Asp Lys Lys Asp Arg Ser Val Lys Ala Ala Met Glu 
                165                 170                 175     


Leu Leu Asn Ile Asn Ala Asn Leu 
            180                 


<210>  8
<211>  555
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_2797, 4Fe-4S ferredoxin, iron-sulpur binding 
       domain-containing protein

<400>  8
atgaaaccag agtttaattc ttttgtaata gccgatcctg acaagtgcat aggctgtaga       60

tcttgtgaga ttgcctgtgc tgcaaaacat agagaagata ctcaaggaaa aaccattgga      120

actatgaata ataaagttac tccaaggtta ttctttgtta aaaataaagg aaatgtaatg      180

ccagtacaat gcagacattg tgaggatgca ccatgtctaa atgcctgccc agttaatgct      240

atagttgaaa aagatggaag tatcattata aatgaaagtg catgtatagg atgtcagacc      300

tgtacaatag tatgtccggt aggtgctgta agtttactgc ctagaactca aggtaaagta      360

gttacaggag gaattcaggt taaagtaaga gcagcagctt ataaatgtga tttatgtaag      420

gaagagggag gagaacctgc ttgcgtcaaa gaatgtccaa aagaggcctt gaggttagta      480

gatcctagag aagataaaaa agatcgtagt gtgaaagctg ctatggaact gttaaatata      540

aacgcaaatc tctaa                                                       555


<210>  9
<211>  462
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_2798, hydrogenase, Fe-only (EC:1.12.7.2), WP_035788218.1

<400>  9

Met Pro Thr Ser Thr Ser Met Ile Asn Ile Asp Glu Glu Leu Cys Thr 
1               5                   10                  15      


Gly Cys Arg Arg Cys Ala Asp Val Cys Pro Val Asp Ala Ile Glu Gly 
            20                  25                  30          


Glu Gln Gly Lys Pro Gln Lys Ile Asn Thr Glu Lys Cys Val Met Cys 
        35                  40                  45              


Gly Gln Cys Ile Gln Val Cys Lys Gly Tyr Gln Ser Val Tyr Asp Asp 
    50                  55                  60                  


Val Pro Thr Pro Val Ser Lys Arg Leu Phe Asp Arg Gly Leu Leu Lys 
65                  70                  75                  80  


Glu Val Asp Glu Pro Leu Phe Ala Ala Tyr Asn Lys Gly Gln Val Lys 
                85                  90                  95      


Ser Val Lys Glu Ile Leu Gln Asn Lys Asp Val Phe Lys Ile Val Gln 
            100                 105                 110         


Cys Ala Pro Ala Val Arg Val Ala Ile Gly Glu Asp Phe Gly Met Pro 
        115                 120                 125             


Leu Gly Thr Leu Ser Glu Gly Lys Met Ala Ala Ala Leu Arg Lys Leu 
    130                 135                 140                 


Gly Phe Asp Lys Val Tyr Asp Thr Asn Phe Gly Ala Asp Leu Thr Ile 
145                 150                 155                 160 


Met Glu Glu Gly Ser Glu Leu Leu Lys Arg Val Ala Glu Gly Gly Val 
                165                 170                 175     


Leu Pro Met Phe Thr Ser Cys Cys Pro Ala Trp Val Lys Tyr Ala Glu 
            180                 185                 190         


Gln Thr Tyr Pro Glu Leu Leu Pro His Leu Ser Ser Cys Lys Ser Pro 
        195                 200                 205             


Asn Gln Met Ala Gly Ala Ile Phe Lys Thr Tyr Gly Ala Glu Ile Asn 
    210                 215                 220                 


Lys Val Asn Pro Ala Lys Ile Tyr Asn Val Ser Val Met Pro Cys Thr 
225                 230                 235                 240 


Cys Lys Glu Phe Glu Ser Glu Arg Glu Glu Met His Asp Ser Gly His 
                245                 250                 255     


Arg Asp Val Asp Ala Val Ile Thr Thr Arg Glu Leu Ala Gln Leu Phe 
            260                 265                 270         


Lys Asp Ala Asp Ile Asp Phe Asn Thr Ile Glu Glu Glu Gln Phe Asp 
        275                 280                 285             


Thr Pro Leu Gly Met Tyr Thr Gly Ala Gly Thr Ile Phe Gly Ala Thr 
    290                 295                 300                 


Gly Gly Val Met Glu Ala Ala Leu Arg Thr Gly Tyr Glu Leu Tyr Thr 
305                 310                 315                 320 


Lys Lys Thr Ile Pro Ser Ile Asp Leu Thr Met Val Arg Gly Gly Glu 
                325                 330                 335     


Gly Phe Arg Thr Ala Glu Val Asp Leu Gly Asp Ile Arg Leu Lys Val 
            340                 345                 350         


Gly Val Val Ser Gly Leu Lys Asn Val Lys Asp Val Met Glu Ser Val 
        355                 360                 365             


Lys Ala Gly Lys Cys Asp Leu His Phe Ile Glu Val Met Thr Cys Pro 
    370                 375                 380                 


Gln Gly Cys Ile Ser Gly Gly Gly Gln Pro Lys Val Ile Leu Asp Ser 
385                 390                 395                 400 


Asp Lys Glu Glu Ala Tyr Asn Asn Arg Lys Lys Gly Leu Tyr Asn His 
                405                 410                 415     


Asp Ala Asn Leu Thr Tyr Arg Lys Ser His Glu Asn Pro Glu Ile Lys 
            420                 425                 430         


Lys Ile Tyr Asp Glu Phe Leu Asp Lys Pro Leu Gly Ala Lys Ser His 
        435                 440                 445             


Glu Leu Leu His Thr Lys Tyr Ile Ser Arg Lys Lys Glu Ser 
    450                 455                 460         


<210>  10
<211>  1389
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_2798, hydrogenase, Fe-only (EC:1.12.7.2)

<400>  10
atgccaacta gtacttctat gataaatata gatgaagaat tatgtacagg ctgcagacga       60

tgtgcggatg tctgccctgt agatgctata gaaggtgaac agggtaaacc tcagaagata      120

aatactgaaa agtgtgttat gtgcggacaa tgcattcaag tttgtaaagg ctatcaatct      180

gtatacgacg atgttcctac tccagttagc aaaaggttat ttgatagagg attgttaaag      240

gaagtagatg aaccattatt tgcagcatat aataaaggtc aggtaaagag tgttaaagaa      300

attttacaaa acaaagatgt atttaaaatt gtgcaatgtg cacctgctgt aagagttgct      360

ataggagagg attttggaat gcctcttgga actttaagtg aaggaaaaat ggcagctgca      420

ctcagaaaat taggatttga caaagtatat gatacaaact ttggtgcaga tcttactata      480

atggaagaag gtagtgagtt actaaaaaga gtagctgaag gcggagtttt gccaatgttt      540

acttcttgtt gtccagcatg ggtaaaatat gcagaacaaa catatccaga acttttacct      600

catctttcaa gttgtaagtc tccaaatcag atggctggag ctatatttaa aacttatgga      660

gcagagataa ataaggttaa tccggctaaa atttataatg tatctgttat gccatgtaca      720

tgcaaggaat ttgaaagtga aagagaagaa atgcatgaca gtggacacag ggatgtagat      780

gcagttataa ctacaaggga attagcacaa ctgttcaaag atgctgatat agattttaat      840

actattgaag aagaacagtt tgatactcct cttggtatgt ataccggtgc aggaactata      900

tttggtgcta caggtggagt tatggaagca gcacttagaa ctggatatga actttatact      960

aaaaaaacta ttccaagtat agatcttact atggtaagag gtggagaagg ttttagaact     1020

gctgaagtag atttagggga tattagacta aaagtaggag tagtttccgg cttaaaaaat     1080

gtaaaagacg ttatggaatc agtaaaggca ggcaaatgtg atttgcactt tatagaggtt     1140

atgacctgtc ctcaaggatg tataagtggt ggaggacaac ctaaagttat acttgattca     1200

gataaagagg aagcttataa taataggaaa aagggactat ataatcatga cgctaatctt     1260

acttatagaa aatcacatga aaatccagaa ataaagaaaa tatatgatga gttcttagac     1320

aaaccattag gagctaagtc tcatgaatta ttgcacacta aatatatctc aagaaaaaag     1380

gagagttaa                                                             1389


<210>  11
<211>  205
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_2799, 4Fe-4S ferredoxin, iron-sulpur binding 
       domain-containing protein

<400>  11

Met Asn Tyr Cys Thr Leu Asn Ile Ser Gln Glu Lys Arg Arg Val Asn 
1               5                   10                  15      


Lys Met Lys Asn Cys Leu Val Val Ala Asp Pro Asn Lys Cys Ile Gly 
            20                  25                  30          


Cys Arg Thr Cys Glu Ala Ala Cys Gly Ile Ala His Ser Gly Gly Asp 
        35                  40                  45              


Phe Phe Asn Thr Asn Val Ser Lys Ile Asn Phe Asn Pro Arg Leu Asn 
    50                  55                  60                  


Val Ile Lys Thr Ala Lys Val Ser Ala Pro Val Gln Cys Arg Gln Cys 
65                  70                  75                  80  


Glu Asp Ala Pro Cys Gly Lys Ala Cys Pro Val Asn Ala Ile Ser Asn 
                85                  90                  95      


Glu Asn Gly Tyr Val Ser Val Asn Lys Asp Val Cys Val Gly Cys Lys 
            100                 105                 110         


Ile Cys Met Leu Ala Cys Pro Phe Gly Ala Ile Glu Leu Ala Ser Gln 
        115                 120                 125             


Tyr Arg Asp Gly Glu Val Val Asp Gln Lys Gly Leu Lys Met Ser Glu 
    130                 135                 140                 


Glu Gly Asn Pro Thr Val Asn Gly Lys Gly Arg Val Val Ala Asn Lys 
145                 150                 155                 160 


Cys Asp Leu Cys Gln Asp Arg Asp Gly Gly Pro Ala Cys Ile Glu Val 
                165                 170                 175     


Cys Pro Thr Lys Ser Leu Lys Leu Val Thr Tyr Asp Asp Asn Asn Asn 
            180                 185                 190         


Ile Val Glu Lys Lys Asp Asp Asp Glu Arg Glu Val Ser 
        195                 200                 205 


<210>  12
<211>  618
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_2799, 4Fe-4S ferredoxin, iron-sulpur binding 
       domain-containing protein

<400>  12
atgaattatt gcacactaaa tatatctcaa gaaaaaagga gagttaataa aatgaagaat       60

tgcctcgtag tagcagatcc taataaatgc ataggatgta ggacttgtga agcagcttgt      120

ggtattgcac attcaggagg ggactttttt aacacaaatg tatccaagat taattttaat      180

cctcgcttaa atgtgataaa aactgctaaa gtaagtgctc ctgttcaatg cagacaatgc      240

gaagatgcac cttgtggtaa agcttgtcca gttaacgcta tttcaaatga aaatggttat      300

gttagtgtaa ataaagatgt atgtgttgga tgtaaaatct gcatgttagc ttgtcctttt      360

ggagctattg aattagcttc tcaatatagg gatggagaag ttgtagacca aaagggactt      420

aagatgagtg aggaaggtaa tcctactgtg aatggaaaag gaagagtggt agcaaataag      480

tgtgatctct gccaggatag ggatggaggg cctgcttgta tagaagtttg tcctacaaaa      540

tctctcaaac tagttactta tgatgacaat aataatatag ttgaaaaaaa agatgacgac      600

gaacgtgaag taagctaa                                                    618


<210>  13
<211>  346
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_1691, (FeFe)-hydrogenase maturation HydE, radical SAM

<400>  13

Met Gly Asp Lys Leu Leu Glu Ser Ile Glu Lys Ala Glu Leu Asn His 
1               5                   10                  15      


Thr Leu Asp Lys Ser Glu Ile Val Glu Leu Phe Asn Ser Asn Glu His 
            20                  25                  30          


Asn Glu Glu Leu Phe Lys Ala Ala Asp Arg Val Arg Lys Lys Tyr Val 
        35                  40                  45              


Gly Asp Glu Val His Leu Arg Gly Leu Ile Glu Phe Ser Asn Ile Cys 
    50                  55                  60                  


Lys Arg Asn Cys Met Tyr Cys Gly Leu Arg Arg Asp Asn Lys Asn Ile 
65                  70                  75                  80  


Lys Arg Tyr Arg Ile Glu Pro Asp Lys Ile Ile Glu Leu Ala Lys Lys 
                85                  90                  95      


Ala Val Gly Tyr Gly Tyr Lys Thr Val Val Leu Gln Ser Gly Glu Asp 
            100                 105                 110         


Asp Tyr Tyr Thr Val Asp Lys Leu Lys Tyr Ile Ile Ser Asn Met Lys 
        115                 120                 125             


Lys Met Asp Ile Ala Val Thr Leu Ser Ile Gly Glu Lys Thr Phe Glu 
    130                 135                 140                 


Glu Tyr Lys Ala Phe Lys Glu Ala Gly Ala Asp Arg Tyr Leu Ile Arg 
145                 150                 155                 160 


Ile Glu Thr Thr Asp Pro Glu Leu Tyr Ala Lys Met Asp Pro Gly Met 
                165                 170                 175     


Ser Tyr Glu Asn Arg Lys Arg Cys Leu Lys Asp Leu Gly Lys Leu Gly 
            180                 185                 190         


Tyr Glu Val Gly Thr Gly Cys Leu Ile Gly Leu Pro Gly Gln Thr Phe 
        195                 200                 205             


Glu Ser Leu Ala Glu Asp Ile Leu Phe Phe Lys Glu Ile Asp Ala Asp 
    210                 215                 220                 


Met Val Gly Val Gly Pro Phe Ile Pro Asn Ala Asp Thr Pro Leu Arg 
225                 230                 235                 240 


Asp Glu Lys Gly Gly Thr Phe Ile Asn Ala Leu Lys Val Met Ala Ile 
                245                 250                 255     


Ser Arg Leu Ile Met Pro Asp Ile Asn Leu Pro Gly Thr Thr Ala Met 
            260                 265                 270         


Glu Thr Leu Asn Pro Arg Gly Arg Thr Ile Ala Leu Gln Ser Gly Ala 
        275                 280                 285             


Asn Val Val Met Pro Asn Val Thr Glu Gly Val Tyr Arg Lys Leu Tyr 
    290                 295                 300                 


Ala Leu Tyr Pro Gly Lys Ile Cys Thr Gly Asp Thr Pro Ala Gln Cys 
305                 310                 315                 320 


Arg Asp Cys Ile Thr Gly Lys Ile Ile Thr Ile Gly Arg Val Ile Ser 
                325                 330                 335     


Gly Ser Lys Gly Phe Arg Val Lys Asn Ser 
            340                 345     


<210>  14
<211>  1041
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_1691, (FeFe)-hydrogenase maturation HydE, radical SAM

<400>  14
atgggggata aattactaga atcaattgaa aaagctgaat tgaatcatac acttgataaa       60

agtgaaatag ttgaattatt taacagtaac gaacataatg aagaactttt taaagcggca      120

gacagagtaa gaaaaaaata tgtaggggat gaagtacatt taagaggact gatagaattt      180

tctaatatat gcaaaagaaa ttgcatgtac tgtggactta gacgtgataa taaaaatatc      240

aaaagatata gaatagagcc ggataaaata attgaattag caaaaaaagc agtaggatac      300

ggctataaaa cagttgtttt gcagtctgga gaagacgatt actatactgt agataagcta      360

aaatacataa tatcaaatat gaagaaaatg gacatagctg taactctcag cataggtgaa      420

aaaacttttg aggagtataa agcttttaaa gaagctggag cagatagata tttaataaga      480

atagaaacta cggatccgga actgtatgca aaaatggacc ctgggatgag ttatgaaaat      540

agaaaacgct gcttgaaaga tcttggaaag ttaggatatg aagttggtac aggatgtctt      600

ataggtcttc ctggccaaac ttttgagtcg cttgcagagg atatattatt ttttaaagaa      660

atagatgcag atatggtagg agtagggcct tttattccaa atgcagatac gcctttaaga      720

gatgaaaaag gtggaacgtt tataaatgct cttaaagtta tggctataag cagactaatt      780

atgccagata ttaatcttcc tggtacaaca gctatggaaa ctttaaatcc aagaggcagg      840

actatagctc ttcagagtgg agctaatgta gttatgccaa atgtaacgga aggtgtatat      900

aggaagcttt atgcactata tccaggtaaa atttgcacag gggatactcc tgcccaatgc      960

agagattgta taaccggtaa aataattact ataggaaggg tcatatctgg ttctaaagga     1020

tttagagtaa aaaatagttg a                                               1041


<210>  15
<211>  411
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_2063, (FeFe)-hydrogenase H-cluster maturation GTPase HydF

<400>  15

Met Ser Leu Asn Glu Thr Pro Arg Ser Val Arg Thr His Ile Ala Leu 
1               5                   10                  15      


Phe Gly Lys Arg Asn Ala Gly Lys Ser Ser Ile Ile Asn Ala Leu Thr 
            20                  25                  30          


Gly Gln Asp Ile Ala Ile Val Ser Asp Val Arg Gly Thr Thr Thr Asp 
        35                  40                  45              


Pro Val Tyr Lys Ser Met Glu Ile Leu Pro Ile Gly Pro Cys Val Ile 
    50                  55                  60                  


Ile Asp Thr Ala Gly Leu Asp Asp Glu Gly Glu Leu Gly Glu Leu Arg 
65                  70                  75                  80  


Lys Glu Lys Thr Leu Ser Val Leu Asn Lys Thr Asp Ile Ser Ile Ile 
                85                  90                  95      


Val Ile Asp Ser Thr Val Gly Ile Thr Asp Tyr Asp Glu Ser Ile Ile 
            100                 105                 110         


Asn Gln Ile Lys Asn Lys Lys Ile Pro Leu Ile Gly Val Leu Asn Lys 
        115                 120                 125             


Ile Asp Ala Ala Asp Ile Lys Asp Leu Asp Val Glu His Met Lys Lys 
    130                 135                 140                 


Glu Leu Lys Ile Pro Ile Val Lys Val Ser Ala Leu Lys Arg Lys Gly 
145                 150                 155                 160 


Ile Leu Glu Leu Lys Asn Gln Ile Ile Ala Ala Gln Pro Gln Ser Glu 
                165                 170                 175     


Asp Lys Phe Lys Val Ile Gly Asp Leu Ile Asn Pro Gly Asp Phe Val 
            180                 185                 190         


Val Leu Val Thr Pro Ile Asp Lys Ala Ala Pro Lys Gly Arg Leu Ile 
        195                 200                 205             


Leu Pro Gln Gln Gln Thr Ile Arg Asp Ile Leu Glu Ser Asp Ala Thr 
    210                 215                 220                 


Ala Val Val Thr Lys Glu Phe Glu Leu Arg Glu Thr Leu Gln Asn Leu 
225                 230                 235                 240 


Gly Lys Lys Pro Lys Ile Val Val Thr Asp Ser Gln Ala Phe Leu Lys 
                245                 250                 255     


Val Ala Ala Asp Thr Pro Lys Asp Ile Leu Met Thr Ser Phe Ser Ile 
            260                 265                 270         


Leu Phe Ala Arg Cys Lys Gly Asp Leu Val Glu Leu Ile Lys Gly Val 
        275                 280                 285             


Lys Ala Val Lys Lys Leu Glu Asp Gly Asp Lys Val Leu Ile Ala Glu 
    290                 295                 300                 


Gly Cys Thr His His Arg Gln Ser Asp Asp Ile Gly Lys Val Lys Ile 
305                 310                 315                 320 


Pro Arg Trp Ile Arg Gln Ile Thr Gly Lys Lys Ile Asp Phe Glu Phe 
                325                 330                 335     


Ser Ser Gly Val Ser Phe Thr Glu Glu Ile Lys Lys Tyr Ala Leu Val 
            340                 345                 350         


Val His Cys Gly Ala Cys Met Leu Asn Arg Ala Ala Met Leu Tyr Arg 
        355                 360                 365             


Ile Asn Thr Ala Lys Glu Leu Asn Val Pro Ile Val Asn Tyr Gly Ile 
    370                 375                 380                 


Leu Ile Ala Tyr Val Gln Gly Ile Leu Asp Arg Ala Leu Lys Pro Phe 
385                 390                 395                 400 


Pro Leu Ala Lys Met Ala Trp Asp Asp Glu Asn 
                405                 410     


<210>  16
<211>  1236
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_2063, (FeFe)-hydrogenase H-cluster maturation GTPase HydF

<400>  16
atgagtttaa atgaaacacc tcgctcagta agaacacata tagcactctt tggcaaaagg       60

aatgcaggca aatcaagtat tataaacgcc ttaacaggac aagatatagc cattgtatca      120

gatgtaaggg gtactactac agatccagtc tataaatcta tggagatact tcccataggc      180

ccctgtgtaa taatagatac cgcaggtctt gatgatgagg gcgagctcgg cgaacttaga      240

aaagaaaaaa cattaagtgt tttaaataaa actgacatat caataattgt aatagattca      300

actgttggaa ttacagatta tgacgagtct ataataaatc aaataaaaaa taaaaaaatt      360

ccccttatag gagtcttaaa caaaatcgat gctgctgata taaaagattt agatgtagaa      420

catatgaaaa aagaactcaa aataccaatt gtaaaagtat ctgcacttaa aagaaaaggt      480

atacttgagt taaaaaatca aataatagct gcgcaacctc aaagtgaaga taaatttaaa      540

gtaattggtg atttaataaa ccctggagac tttgtggttc ttgtaactcc aatagataaa      600

gcagctccta aaggaagact tattctccct cagcagcaaa ccataagaga tatactggaa      660

agtgatgcta ctgcagtggt aactaaagaa ttcgaactta gagaaacctt acaaaaccta      720

gggaaaaaac ctaaaatagt agttacagat tctcaggcat ttttaaaagt agcagcagat      780

accccaaaag atatattgat gacttccttc tccatcctat ttgcaagatg caaaggagac      840

ctagtagaac ttataaaagg agtaaaagct gtaaaaaaat tagaagatgg agacaaagta      900

cttattgcag aaggctgcac tcaccataga caatctgatg atataggtaa agtaaaaatt      960

cctagatgga tacgacaaat taccggtaaa aaaatagatt tcgaattttc atctggcgtt     1020

tcttttacag aagaaataaa gaaatatgcc ttagtagttc actgcggtgc ttgcatgtta     1080

aatagagccg caatgcttta tagaataaat actgcaaaag agcttaatgt ccctattgta     1140

aactatggca tacttattgc atatgtccaa ggtatactag atagagcttt aaaaccattt     1200

ccactagcta aaatggcatg ggatgatgaa aattga                               1236


<210>  17
<211>  324
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_0339, Elongator protein 3/MiaB/NifB

<400>  17

Met Thr Ile Asp Lys Ile Leu Asn Asp Ala Lys Ser Glu Lys Leu Leu 
1               5                   10                  15      


Asn Lys Glu Glu Ala Ile Ala Leu Leu Asn Val Lys Asn Asn Ser Ser 
            20                  25                  30          


Asp Phe Tyr Lys Ile Ile Ser Leu Ala Asn Glu Met Thr Arg Ser Glu 
        35                  40                  45              


Phe Asn Asn Lys Ala Phe Ile Phe Ala Gln Ile Gly Leu Asn Ala Glu 
    50                  55                  60                  


Pro Cys Pro Val Asn Cys Lys Phe Cys Ser Met Gly Lys Asn His Tyr 
65                  70                  75                  80  


Thr Met Glu Ser Thr Trp Arg Lys Asp Ile Asn Ser Ile Leu Ser Glu 
                85                  90                  95      


Thr Lys Ala Ile Val Asn Glu Gly Ile Asn Asp Leu Phe Leu Met Thr 
            100                 105                 110         


Thr Ala Asp Tyr Pro Ile Asn Asp Phe Leu Asn Ile Ala Arg Asn Val 
        115                 120                 125             


Arg Ser Ile Leu Pro Asp Asn Ile Arg Leu Val Ala Asn Ile Gly Asp 
    130                 135                 140                 


Phe Asp Tyr Val Thr Ala Leu Lys Leu Lys Glu Val Gly Phe Thr Gly 
145                 150                 155                 160 


Ala Tyr His Ile Lys Arg Leu Arg Glu Gly Ile Asp Thr Thr Ile Lys 
                165                 170                 175     


Pro Glu Thr Arg Ile Glu Thr Leu Asn Ser Ile Lys Lys Ala Gly Leu 
            180                 185                 190         


Glu Leu Tyr Tyr Cys Val Glu Pro Ile Gly Pro Glu His Ser Tyr Glu 
        195                 200                 205             


Glu Ile Val Asp Glu Met Leu Arg Ala Arg Asp Tyr Asn Val Gly Val 
    210                 215                 220                 


Met Ala Ala Met Arg Arg Ile Pro Val Lys Gly Thr Pro Leu Tyr Glu 
225                 230                 235                 240 


Lys Gly Gln Ile Ser Ser Val Glu Leu Ser Lys Ile Ala Ala Val Thr 
                245                 250                 255     


Arg Ile Val Thr Arg Pro His Arg Ala Met Asn Ala His Glu Thr Ile 
            260                 265                 270         


Gln Met Ser Leu Ile Cys Gly Val Asn Gln Leu Tyr Ala Glu Ala Gly 
        275                 280                 285             


Ala Asn Pro Arg Asp Ser Ile Ser Asn Thr Glu Lys Ser Arg Gly Leu 
    290                 295                 300                 


Ser Val Lys Asn Ile Lys Lys Leu Phe Glu Asp Ala Glu Tyr Glu Ile 
305                 310                 315                 320 


Ser Arg Asp Tyr 
                


<210>  18
<211>  975
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_0339, Elongator protein

<400>  18
atgactatag ataaaatcct taatgatgct aaaagtgaaa aattacttaa taaagaagag       60

gcaatagcat tgttaaatgt aaaaaacaat tccagtgact tttataaaat tatatcactt      120

gcaaacgaaa tgacacgttc tgaatttaat aacaaggctt ttatatttgc ccaaatagga      180

ttaaacgcag aaccgtgtcc tgtaaactgt aaattttgtt ccatgggtaa aaaccattat      240

actatggaaa gtacatggag aaaggatata aactccatac tatctgaaac taaagctatt      300

gttaatgaag gtattaatga cttattccta atgacaactg ctgactatcc aataaatgat      360

ttcttgaata tagcaagaaa tgtaagaagc attttgccag ataatataag gctggtagct      420

aatattgggg actttgacta cgttacagca cttaaattaa aagaggtggg ctttactgga      480

gcataccaca ttaaacgttt aagggaagga atagatacaa ctattaaacc tgaaactaga      540

attgaaactc tcaattcaat aaaaaaagca ggattggaat tatactattg tgtagaacct      600

attggcccag agcacagtta tgaggaaata gttgatgaaa tgttgagagc tagagattat      660

aatgtaggag ttatggctgc aatgagaaga ataccggtta aaggcactcc tttgtatgaa      720

aagggtcaaa tttcatcggt tgagctttct aaaattgcgg cagttacaag aatagttaca      780

cgacctcata gggctatgaa tgcacatgag accattcaga tgagtttaat ttgtggagta      840

aatcagttgt atgctgaagc aggtgctaat ccccgagata gtatatccaa tactgaaaaa      900

agccgtggat tatcggtaaa aaatataaaa aaattatttg aagatgcaga atatgaaata      960

tcaagagatt attag                                                       975


<210>  19
<211>  559
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_1576, hydrogenase, Fe-only (EC:1.12.7.2), WP_023162310

<400>  19

Met Ser Gly Gln Phe Met Ile Ile Asp Asn Ile Pro Val Glu Ile Asn 
1               5                   10                  15      


Gly Glu Lys Asn Ile Leu Glu Leu Ile Arg Lys Ala Gly Ile Asp Leu 
            20                  25                  30          


Pro Thr Phe Cys Tyr His Ser Glu Leu Ser Val Tyr Gly Ala Cys Arg 
        35                  40                  45              


Met Cys Met Val Glu Asp Lys Arg Gly Arg Met Gln Ala Ala Cys Ser 
    50                  55                  60                  


Thr Pro Pro Gln Ala Gly Ile Glu Ile Tyr Thr Asn Thr Pro Arg Leu 
65                  70                  75                  80  


Arg Lys Tyr Arg Lys Asn Ile Leu Glu Leu Leu Leu Ala Asn His Cys 
                85                  90                  95      


Arg Asp Cys Thr Thr Cys Glu Lys Asn Glu His Cys Lys Leu Gln Asp 
            100                 105                 110         


Leu Ala Lys Arg Phe Lys Ile Lys Lys Val Arg Phe Lys Asn Thr Ser 
        115                 120                 125             


Ile Asn Lys Lys Ile Asp Asn Ser Ser Val Cys Ile Val Arg Asn Arg 
    130                 135                 140                 


Ser Lys Cys Ile Leu Cys Gly Asp Cys Val Arg Val Cys Glu Glu Val 
145                 150                 155                 160 


Gln Asn Val Gly Ala Ile Asp Phe Val Lys Arg Gly Ser Asn Met Thr 
                165                 170                 175     


Val Thr Thr Ala Phe Asp Glu Pro Ile Ala Asn Ser Asn Cys Val Gly 
            180                 185                 190         


Cys Gly Gln Cys Ala Ala Val Cys Pro Thr Gly Ala Ile Val Val Lys 
        195                 200                 205             


Asp Asp Thr Ala Glu Leu Trp Glu Ala Leu Ser Asp Lys Asn Thr Lys 
    210                 215                 220                 


Val Val Ala Gln Ile Ala Pro Ala Val Arg Val Gly Leu Asn Glu Glu 
225                 230                 235                 240 


Leu Gly Glu Glu Asn Gly Glu Asn Glu Met Gly Lys Ile Val Ala Ala 
                245                 250                 255     


Leu Arg Arg Met Gly Phe Asp Glu Val Phe Asp Thr Ser Thr Ala Ala 
            260                 265                 270         


Asp Leu Thr Val Leu Glu Glu Thr Ala Glu Phe Thr Ser Arg Leu Glu 
        275                 280                 285             


Lys Asn Glu Ser Leu Pro Leu Phe Thr Ser Cys Cys Ser Ala Trp Val 
    290                 295                 300                 


Asn Tyr Val Glu Asn Thr His Pro Glu Leu Met Lys Tyr Val Ser Thr 
305                 310                 315                 320 


Cys Lys Ser Pro Met Glu Met Phe Ala Ser Val Leu Lys Glu Tyr Tyr 
                325                 330                 335     


Lys Asn Ser Asp Lys Lys Ile Val Val Val Ala Val Met Pro Cys Thr 
            340                 345                 350         


Ala Lys Lys Tyr Glu Ala Lys Arg Glu Glu Phe Ser Lys Asn Gly Val 
        355                 360                 365             


Pro Asp Val Asp Tyr Val Ile Thr Thr Gln Glu Leu Ile Ser Met Ile 
    370                 375                 380                 


Arg Gln Ala Gly Ile Val Phe Pro Glu Leu Glu Pro Glu Ala Val Asp 
385                 390                 395                 400 


Met Pro Phe Asp Leu Ser Ser Gly Ala Gly Val Ile Phe Gly Val Thr 
                405                 410                 415     


Gly Gly Val Thr Glu Ala Val Ile Arg Lys Val Leu Ala Asp Lys Ser 
            420                 425                 430         


Asn Ala Ala Leu Arg Ala Ile Val Phe Asn Gly Val Arg Gly Met Glu 
        435                 440                 445             


Gly Thr Lys Glu Ala Ser Ile Thr Val Gly Asp Arg Glu Ile Lys Ile 
    450                 455                 460                 


Ala Ile Val Ser Gly Leu Arg Asn Ala Glu Asn Leu Ile Gln Lys Ile 
465                 470                 475                 480 


Gln Ser Gly Glu Ser Lys Tyr Asp Phe Val Glu Val Met Ala Cys Pro 
                485                 490                 495     


Gly Gly Cys Ile Ser Gly Gly Gly Gln Pro Phe Glu Lys Leu Glu Gly 
            500                 505                 510         


Lys Leu Lys Arg Ser Ala Gly Ile Tyr Gln Ser Asp Lys Met Ser Thr 
        515                 520                 525             


Ile Lys Arg Thr Ala Asp Asn Pro Leu Met Lys Ser Leu Tyr Ser Gly 
    530                 535                 540                 


Leu Leu Lys Gly Lys Asn His Glu Leu Leu His Val Asn Arg Lys 
545                 550                 555                 


<210>  20
<211>  1680
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_1576, hydrogenase, Fe-only (EC:1.12.7.2)

<400>  20
atgagtggac aatttatgat tatagataat attcctgtag agataaatgg tgaaaaaaat       60

attcttgaac taattagaaa agctggcatt gatttaccta cattttgcta tcattcggag      120

ctttcagttt atggtgcatg ccgtatgtgc atggttgaag ataaacgcgg ccgtatgcaa      180

gctgcatgtt ctactcctcc tcaagctggt atagaaatat atacaaatac tccaagactt      240

agaaaataca gaaaaaacat tcttgagttg ttactcgcaa atcattgcag agattgtaca      300

acttgtgaaa aaaatgagca ttgcaaacta caagatttag caaagcgttt taaaataaag      360

aaagtaagat ttaaaaatac ttctataaat aaaaaaattg ataattcatc agtatgcatt      420

gtaagaaata ggagtaaatg tatcttatgc ggtgactgtg taagagtgtg tgaagaagta      480

caaaatgttg gagctattga ttttgttaaa agaggttcta atatgactgt aactactgca      540

tttgatgaac ctatagcaaa ttcgaattgt gttggatgtg gtcaatgtgc ggcagtatgc      600

cctactggtg ctattgtagt aaaggatgat acagctgaat tatgggaagc acttagtgat      660

aagaatacaa aggttgtagc tcaaattgcg cccgctgtaa gagttggtct taatgaggaa      720

ttaggtgagg aaaacggcga aaacgaaatg ggtaaaatag tagctgcact tagaagaatg      780

ggatttgatg aagtttttga tacttcaacg gcagcagatc ttacagtttt ggaagaaaca      840

gcagaattta cttcaagact tgaaaaaaat gaaagtttac cattgtttac atcctgttgt      900

tctgcatggg taaattatgt agagaataca catccagagt taatgaaata tgtttctact      960

tgcaaatcac ctatggaaat gtttgcttct gtacttaagg agtactataa aaatagtgat     1020

aaaaaaattg tagttgtagc agttatgcct tgtacagcta aaaaatatga agcaaaacga     1080

gaagaatttt caaaaaatgg tgtacctgat gtagattatg taataactac acaggagctt     1140

ataagtatga taagacaagc aggaattgta tttcctgaat tagagcctga agcagttgat     1200

atgccatttg atcttagcag tggagctgga gttatatttg gagtaacagg tggtgttaca     1260

gaggctgtta tacgtaaagt tttagctgat aaatcaaatg ctgcattacg tgcaattgtg     1320

tttaatggtg ttaggggcat ggaaggtact aaagaagcta gcattactgt tggtgatcgt     1380

gaaataaaaa tagcaatagt aagcggtctt agaaatgcag aaaatcttat acagaaaata     1440

caatctggtg aatcaaaata tgatttcgtt gaagttatgg catgtccagg tggatgcatt     1500

tctggtggtg gacaaccatt tgaaaaactt gaaggaaagc taaaacgtag tgctggaata     1560

tatcaatcag ataaaatgag cactataaaa cgtacagctg acaatccgct tatgaaatca     1620

ctgtattcag gattgttaaa aggtaaaaac cacgaactat tacatgtaaa ccgcaaatag     1680


<210>  21
<211>  624
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_1577, NADH dehydrogenase (quinone) (EC:1.6.99.5)

<400>  21

Met Phe Ser Ser Arg Asn Asp Leu Ile Asn Ala Arg Lys Ile Tyr Lys 
1               5                   10                  15      


Asn Ser Leu Glu Met Gln Ser Lys Lys Ile Leu Ile Cys Gly Gly Thr 
            20                  25                  30          


Gly Cys Val Ala Gly Gly Ser Leu Lys Ile Tyr Asp Glu Phe Leu Arg 
        35                  40                  45              


Leu Met Lys Glu Lys Gly Ile Asn Cys Thr Val Glu Leu Glu Lys Glu 
    50                  55                  60                  


Ser His Asp Lys Ser Val Gly Leu Lys Lys Ser Gly Cys His Gly Phe 
65                  70                  75                  80  


Cys Glu Met Gly Pro Leu Val Arg Val Glu Pro Phe Gly Tyr Leu Tyr 
                85                  90                  95      


Ile Gln Val Lys Pro Asp Asp Cys Leu Glu Ile Leu Asp Lys Thr Ile 
            100                 105                 110         


Leu Asn Asp Glu Cys Val Glu Arg Leu Ala Tyr Lys Lys Asp Gly Gln 
        115                 120                 125             


Ile Tyr Arg Lys Gln Gln Glu Ile Pro Phe Tyr Lys Lys Gln Thr Arg 
    130                 135                 140                 


Val Ala Leu Glu His Cys Gly His Ile Asp Ala Thr Ser Ile Lys Glu 
145                 150                 155                 160 


Cys Ile Ala Leu Gly Gly Tyr Ser Ser Phe Glu Lys Ala Leu Phe Asp 
                165                 170                 175     


Met Asp Ser Asp Glu Val Ile Lys Gln Ile Asp Glu Ser Asn Leu Arg 
            180                 185                 190         


Gly Arg Gly Gly Gly Gly Phe Pro Thr Gly Arg Lys Trp Thr Gln Val 
        195                 200                 205             


Lys Arg Gln Lys Thr Asp Met Lys Tyr Ile Val Cys Asn Gly Asp Glu 
    210                 215                 220                 


Gly Asp Pro Gly Ala Phe Met Asp Arg Ser Val Met Glu Gly Asp Pro 
225                 230                 235                 240 


His Arg Val Leu Glu Gly Met Met Ile Ala Gly Val Ala Cys Gly Ala 
                245                 250                 255     


Gln Glu Gly Tyr Ile Tyr Val Arg Ala Glu Tyr Pro Leu Ala Val Glu 
            260                 265                 270         


Arg Leu Ser Asn Ala Ile Ala Gln Ala Lys Glu Tyr Gly Leu Leu Gly 
        275                 280                 285             


Lys Asn Ile Leu Gly Thr Gly Phe Asn Phe Asp Ile Lys Ile Asn Lys 
    290                 295                 300                 


Gly Ala Gly Ala Phe Val Cys Gly Glu Gly Ser Ala Leu Thr Ala Ser 
305                 310                 315                 320 


Ile Glu Gly Lys Arg Gly Met Pro Arg Val Lys Pro Pro Arg Thr Val 
                325                 330                 335     


Glu His Gly Leu Phe Gly Lys Pro Thr Val Leu Asn Asn Val Glu Thr 
            340                 345                 350         


Phe Ala Asn Val Pro Val Ile Ile Ala Lys Gly Ala Asp Trp Tyr Arg 
        355                 360                 365             


Asn Ile Gly Pro Glu Asn Ser Pro Gly Thr Lys Ala Phe Ala Leu Thr 
    370                 375                 380                 


Gly Asn Ile Glu Asn Thr Gly Leu Ile Glu Val Pro Met Gly Thr Thr 
385                 390                 395                 400 


Leu Arg Glu Val Ile Phe Asp Ile Gly Gly Gly Ile Arg Asn Gly Lys 
                405                 410                 415     


Lys Phe Lys Ala Val Gln Ile Gly Gly Pro Ser Gly Gly Cys Leu Thr 
            420                 425                 430         


Ser Lys Asp Leu Asp Leu Pro Leu Asp Phe Asp Ser Leu Lys Lys Val 
        435                 440                 445             


Gly Ala Met Ile Gly Ser Gly Gly Leu Val Val Met Asp Glu Asp Thr 
    450                 455                 460                 


Cys Met Val Asp Thr Ala Lys Phe Phe Met Asn Phe Thr Arg Asn Glu 
465                 470                 475                 480 


Ser Cys Gly Lys Cys Val Pro Cys Arg Glu Gly Thr Lys Arg Met Leu 
                485                 490                 495     


Glu Ile Leu Glu Gly Ile Val Glu Gly Lys Gly Lys Ile Glu Asp Ile 
            500                 505                 510         


Asp Met Leu Leu Glu Leu Ala Asp Thr Ile Ser Ser Thr Ala Leu Cys 
        515                 520                 525             


Gly Leu Gly Lys Ser Ala Pro Ser Pro Val Val Ser Thr Ile Lys Asn 
    530                 535                 540                 


Phe Arg Asp Glu Tyr Glu Thr His Ile Val Asp Lys Lys Cys Pro Ser 
545                 550                 555                 560 


Lys Thr Cys Thr Lys Leu Arg Thr Ile Tyr Ile Asp Lys Thr Ile Cys 
                565                 570                 575     


Lys Gly Cys Ser Lys Cys Ser Arg Ala Cys Pro Val Gly Ala Ile Ser 
            580                 585                 590         


Gly Thr Ile Lys Lys Pro Phe Thr Ile Asp Gln Asn Lys Cys Ile Lys 
        595                 600                 605             


Cys Gly Thr Cys Val Asp Thr Cys Ala Phe Lys Ala Val Lys Glu Asp 
    610                 615                 620                 


<210>  22
<211>  1875
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_1577, NADH dehydrogenase (quinone) (EC:1.6.99.5)

<400>  22
atgtttagta gtagaaatga tttgattaat gctagaaaaa tttataaaaa tagccttgaa       60

atgcaaagta agaaaattct catatgtggt ggtactggat gtgttgcagg tggttcatta      120

aaaatttatg atgaattttt aagattgatg aaagaaaaag gaataaattg tacagtagaa      180

cttgaaaaag agtcgcatga taaatcagtt ggacttaaga aaagtggatg ccatggattt      240

tgtgagatgg gacctcttgt aagagttgaa cctttcggat acttgtatat acaagtaaaa      300

ccggatgact gtttagaaat tttagataaa actatactta atgatgaatg tgttgaacga      360

cttgcatata aaaaagatgg acaaatatat aggaagcagc aggaaattcc tttttataaa      420

aaacaaacaa gagttgccct tgagcactgt ggtcacattg atgcaacttc aataaaagaa      480

tgtattgcct taggaggata ttcctcattt gaaaaagcat tatttgacat ggattcggat      540

gaagttatta agcaaataga tgaatcaaat ttaagaggac gtggaggcgg tggatttcct      600

actggccgta aatggactca agtaaaacgt caaaagacag atatgaaata tattgtatgt      660

aatggtgacg aaggggatcc aggtgcattc atggatagaa gcgtaatgga aggagatcct      720

catagagtac ttgaaggaat gatgattgca ggtgttgctt gtggtgcaca agagggatat      780

atttatgttc gtgcagaata tcctcttgct gtagagagac tttctaatgc aattgcacaa      840

gcaaaagaat acggactttt gggtaaaaat attcttggaa caggttttaa ctttgatatt      900

aaaattaata aaggagcagg tgcttttgtc tgtggtgaag gaagtgccct tacagcttct      960

attgaaggaa aaagaggtat gccaagagta aaacctccaa gaactgttga acatggactc     1020

tttggtaagc caacagtact taataacgtt gaaacttttg caaatgtacc tgtaattatt     1080

gctaaaggag cagactggta tagaaacatt ggacctgaaa acagtcctgg tacaaaagcc     1140

tttgctttaa caggaaatat tgaaaataca ggattaattg aagtaccaat gggtacaacc     1200

ctaagagaag taatatttga tattggtgga ggaataagaa acggtaaaaa atttaaggct     1260

gtacaaatag gagggccatc tggcggatgt cttacaagta aagatcttga tttgccactg     1320

gattttgatt cacttaaaaa agtaggagcc atgataggtt ctggcggact tgtagttatg     1380

gatgaagata catgtatggt tgatactgca aaatttttca tgaactttac acgaaatgaa     1440

tcctgtggaa aatgtgttcc ttgccgtgaa ggaactaaga gaatgcttga aatccttgag     1500

ggcatagttg aaggcaaagg taaaatagaa gacatagata tgttattaga gctagcagat     1560

actatatctt caacagcact atgcggactt gggaaatcgg ctccatcacc agttgttagt     1620

actattaaga atttccgtga tgaatatgaa actcacatag ttgataaaaa atgcccatca     1680

aaaacatgta ctaagcttag aacaatatat atagataaga caatttgtaa aggatgctca     1740

aaatgttcaa gagcttgtcc tgtcggagca atttcaggaa caatcaagaa gccatttaca     1800

attgatcaaa ataaatgtat aaaatgtgga acctgtgttg atacttgtgc atttaaagct     1860

gtaaaggagg attaa                                                      1875


<210>  23
<211>  158
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_1578, NADH dehydrogenase (ubiquinone) 24 kDa subunit

<400>  23

Met Asn Lys Ser Phe Asp Tyr Ser Lys Leu Asp Thr Ile Leu Lys Asn 
1               5                   10                  15      


His Glu Phe Lys Ser Ser Asn Ile Ile Ala Ile Leu Gln Asp Ile Gln 
            20                  25                  30          


Gly Ile Tyr Arg Tyr Leu Pro Lys Glu Ile Phe Pro Tyr Leu Ser Lys 
        35                  40                  45              


Asn Leu Gly Val Ser Lys Ala Lys Ile Tyr Gly Ile Ala Thr Phe Tyr 
    50                  55                  60                  


Glu Asn Phe Ser Leu Glu Pro Lys Gly Lys Tyr Val Ile Lys Val Cys 
65                  70                  75                  80  


Asn Gly Thr Ala Cys His Val Arg Gly Ser Ile Pro Ile Leu Asn Ile 
                85                  90                  95      


Leu Arg Lys Glu Leu Lys Leu Ser Asp Thr Lys Thr Thr Thr Asp Asp 
            100                 105                 110         


Leu Met Phe Ser Leu Glu Thr Val Ser Cys Leu Gly Ala Cys Gly Leu 
        115                 120                 125             


Ala Pro Ala Ile Thr Ile Asn Asp Lys Val His Gly Ser Met Thr Pro 
    130                 135                 140                 


Asp Lys Ala Met Glu Leu Leu Asn Ser Leu Lys Glu Glu Lys 
145                 150                 155             


<210>  24
<211>  477
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_1578, NADH dehydrogenase (ubiquinone) 24 kDa subunit

<400>  24
atgaataagt catttgatta cagtaagtta gatactatat tgaaaaatca tgaatttaaa       60

tctagcaata ttattgcaat attgcaggac atccaaggaa tatacaggta tttacctaag      120

gagatatttc cgtatctttc aaaaaatctt ggagttagca aagcgaagat atatggtata      180

gcaacctttt atgaaaattt ttctttagaa cctaaaggta aatacgtaat aaaagtatgt      240

aatggtactg cttgccatgt aagaggatct atacctatat tgaatatact aagaaaggaa      300

ttaaagcttt cagatactaa aactacaact gatgatttaa tgttttcttt ggaaacggtt      360

tcttgtcttg gcgcttgcgg actagcccct gctatcacaa ttaatgacaa ggtacatggt      420

tctatgactc ctgataaagc aatggaactt ttaaactcac ttaaggagga aaaatag         477


<210>  25
<211>  578
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_3569, hydrogenase, Fe-only (EC:1.12.7.2), WP_023163243

<400>  25

Met Val Asn Leu Thr Ile Asn Asp Ile Lys Val Ser Val Pro Glu Gly 
1               5                   10                  15      


Thr Thr Ile Leu Asn Ala Ala Lys Lys Val Asn Ile Asn Ile Pro Thr 
            20                  25                  30          


Leu Cys Tyr Leu Asp Leu His Asp Ile Lys Met Val Asn Arg Thr Ser 
        35                  40                  45              


Ser Cys Arg Val Cys Leu Val Glu Ile Glu Gly Arg Arg Asn Leu Ala 
    50                  55                  60                  


Pro Ser Cys Ser Thr Glu Ala Phe Glu Gly Met Ile Val Arg Thr Asn 
65                  70                  75                  80  


Ser Ala Arg Ala Ile Lys Ala Arg Arg Thr Met Val Glu Leu Leu Leu 
                85                  90                  95      


Ser Asp His Pro Thr Asp Cys Leu Val Cys Glu Lys Asn Thr Gln Cys 
            100                 105                 110         


Gln Leu Gln Leu Ile Ala Ala Glu Leu Gly Ile Arg Lys Ile Arg Tyr 
        115                 120                 125             


Lys Gly Ala Met Ser Asn Tyr Lys Lys Asp Ser Ser Ser Gly Ala Leu 
    130                 135                 140                 


Tyr Arg Asn Leu Asp Lys Cys Ile Met Cys Arg Arg Cys Glu Thr Met 
145                 150                 155                 160 


Cys Asn Glu Val Gln Thr Cys Gln Val Tyr Ser Ala Val Asp Arg Gly 
                165                 170                 175     


Phe Glu Thr Val Val Ser Pro Ala Phe Gly Arg Pro Met Val Asp Thr 
            180                 185                 190         


Gln Cys Thr Phe Cys Gly Gln Cys Val Ser Val Cys Pro Thr Ala Ala 
        195                 200                 205             


Leu Thr Gln Val Ser Asn Val Ala Lys Val Trp Glu Val Leu Thr Asp 
    210                 215                 220                 


Pro Asp Lys Tyr Val Val Val Gln Thr Ala Pro Ala Ile Arg Val Thr 
225                 230                 235                 240 


Leu Gly Glu Lys Phe Gly Met Glu Pro Gly Thr Ile Val Thr Gly Lys 
                245                 250                 255     


Met Val Ala Ala Leu Arg Arg Leu Gly Phe Asp Lys Val Cys Asp Thr 
            260                 265                 270         


Asp Phe Ala Ala Asp Val Thr Ile Leu Glu Glu Ala His Glu Phe Ile 
        275                 280                 285             


Asp Arg Leu Gln Asn Gly Gly Arg Leu Pro Ile Leu Thr Ser Cys Cys 
    290                 295                 300                 


Pro Ser Trp Val Lys Phe Ile Glu His Gln Phe Pro Asp Leu Leu Asp 
305                 310                 315                 320 


Ile Pro Ser Thr Cys Lys Ser Pro His Ile Met Phe Gly Thr Leu Ala 
                325                 330                 335     


Lys Thr Tyr Met Ala Glu Lys Leu Asn Ile Asp Pro Ser Lys Ile Val 
            340                 345                 350         


Val Val Ser Val Met Pro Cys Ile Ala Lys Lys Tyr Glu Ile Ser Arg 
        355                 360                 365             


Lys Glu Leu Gln Tyr Glu Gly His Lys Asn Val Asp Leu Val Val Thr 
    370                 375                 380                 


Thr Arg Glu Leu Ala Asp Met Ile Met Glu Ala Gly Ile Asp Phe Asn 
385                 390                 395                 400 


Lys Leu Pro Asp Glu Asp Phe Asp Asn Pro Leu Gly Glu Ser Thr Gly 
                405                 410                 415     


Ala Ser Val Ile Phe Gly Thr Thr Gly Gly Val Ile Glu Ala Ala Leu 
            420                 425                 430         


Arg Thr Ala Tyr Glu Trp Ile Thr Gly Glu Thr Leu Lys Glu Val Glu 
        435                 440                 445             


Phe His Ser Val Arg Gly Leu Asp Gly Leu Lys Glu Ala Ser Ile Asn 
    450                 455                 460                 


Ile Gly Gly Lys Lys Ile Asn Ile Gly Val Ala His Gly Leu Gly Asn 
465                 470                 475                 480 


Ala Arg Lys Leu Leu Glu Glu Ile Glu Ser Gly Glu Ser Lys Tyr His 
                485                 490                 495     


Ala Ile Glu Ile Met Ala Cys Pro Gly Gly Cys Ile Asp Gly Gly Gly 
            500                 505                 510         


Gln Pro Tyr His Phe Gly Asp Leu Asp Ile Val Lys Lys Arg Met Glu 
        515                 520                 525             


Ala Leu Tyr Arg Glu Asp Arg Asn Lys Pro Leu Arg Lys Ser His Glu 
    530                 535                 540                 


Asn Pro Glu Val Gln Ala Leu Tyr Lys Glu Phe Ile Gly Asp Val Gly 
545                 550                 555                 560 


Gly Lys Lys Ala His Asp Leu Leu His Thr His Tyr Ile Lys Arg Gln 
                565                 570                 575     


Lys Leu 
        


<210>  26
<211>  1737
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_3569, hydrogenase, Fe-only (EC:1.12.7.2)

<400>  26
atggtaaatt taactataaa cgatataaag gtttctgtcc cagaaggcac tacaatttta       60

aacgctgcaa aaaaagtaaa cataaatata cctactctct gctatcttga tcttcacgat      120

ataaaaatgg taaatagaac ttcctcctgc agagtttgtc ttgttgaaat tgaaggaaga      180

cgaaatcttg caccttcatg ttctacagaa gctttcgagg gtatgatagt tagaacaaac      240

agtgccagag ctataaaagc aaggcgtacc atggtagaac ttttattatc agatcatcct      300

accgactgcc ttgtatgtga aaagaatact caatgccaac ttcaattaat tgctgctgaa      360

ttaggtataa ggaaaataag atataaaggt gctatgtcta attataagaa ggattcctcc      420

agcggtgctc tatatagaaa tctggataag tgcataatgt gcagacgatg cgaaaccatg      480

tgcaatgaag ttcaaacctg tcaggtttac tctgcagtag atagaggctt cgaaactgta      540

gtatcccctg catttggtcg tcctatggtt gacacgcaat gcacattttg cggtcaatgt      600

gtatcagtat gtccaaccgc tgcattaact caagttagta atgtagctaa ggtatgggaa      660

gtactaactg atcctgataa atatgtagta gttcaaactg cccctgctat aagagttact      720

ttaggtgaaa aattcggtat ggaacctgga actattgtaa ctggcaaaat ggttgcagcc      780

ttaagaagat tgggttttga taaggtatgt gatacagact ttgcagcaga tgtaactatt      840

ttagaagaag ctcatgaatt tatagataga cttcaaaatg gtggaagact tccaatactc      900

acaagctgct gtcccagctg ggttaaattt atagaacatc aatttcctga tcttttagat      960

ataccttcaa cttgtaaatc tccacacata atgtttggta ctttagctaa aacatatatg     1020

gcagaaaaat taaatattga tccatctaaa attgtagtag tttcagttat gccctgtatt     1080

gcaaaaaaat atgaaataag cagaaaagag cttcaatatg aaggtcataa aaatgttgat     1140

cttgtggtta ccacaagaga gcttgcagat atgataatgg aagcaggaat agattttaac     1200

aaacttcctg atgaagattt tgataatcca cttggagaat ccacaggtgc ctctgtaata     1260

tttggaacta ccggcggcgt aattgaagca gctcttagaa ctgcttatga atggattact     1320

ggagagactt taaaagaagt agaatttcat agtgtaagag gtcttgacgg acttaaagaa     1380

gccagtataa atattggtgg taaaaaaata aacatcggtg tagcacacgg tcttggcaac     1440

gcaagaaaac ttcttgagga aatagaatct ggtgaatcaa aatatcatgc tatagaaata     1500

atggcctgtc ctggaggatg tattgacgga ggaggtcagc catatcactt tggagattta     1560

gatatcgtaa agaaaagaat ggaagcttta tatagagaag atagaaacaa acctctcaga     1620

aaatctcatg aaaatcctga agttcaagct ctatataaag aatttattgg tgatgtaggt     1680

ggaaaaaaag ctcatgatct ccttcacacc cattatataa aaaggcaaaa attataa        1737


<210>  27
<211>  626
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_3570, NADH dehydrogenase (quinone) (EC:1.6.99.5)

<400>  27

Met Asp Lys Ile Lys Ser Phe Glu Asp Leu Lys Thr Leu Arg Glu Lys 
1               5                   10                  15      


Tyr Lys Ala Lys Ile Ala Asn Arg Thr Tyr Asp Asn Ala Asp Lys Asn 
            20                  25                  30          


Ile Lys Lys Thr Leu Leu Val Cys Gly Gly Thr Gly Cys Arg Ala Ser 
        35                  40                  45              


Arg Ser Leu Asp Ile Val Asn Ile Leu Glu Thr Glu Ile Lys Asn Ala 
    50                  55                  60                  


Gly Leu Glu Asn Thr Val Asp Val Ile Ser Thr Gly Cys Phe Gly Phe 
65                  70                  75                  80  


Cys Glu Lys Gly Pro Ile Val Lys Val Val Pro Asp Asn Val Phe Tyr 
                85                  90                  95      


Val Glu Val Asn Thr Glu Arg Ala Lys Leu Ile Val Tyr Glu His Met 
            100                 105                 110         


Ala Lys Asp Thr Val Val Glu Glu Ala Leu Tyr Lys Asp Pro Ile Thr 
        115                 120                 125             


Asn Glu Lys Ile Ser Asn Gln Thr Asp Ile Pro Phe Tyr Lys Asn Gln 
    130                 135                 140                 


Lys Arg Ile Ala Leu Arg Asn Cys Gly Leu Leu Asn Pro Glu Asp Ile 
145                 150                 155                 160 


Thr Glu Tyr Ile Ala Leu Asn Gly Tyr Glu Ala Leu Gly Lys Val Leu 
                165                 170                 175     


Thr Gln Met Thr Pro Asp Ser Val Ile Asp Glu Ile Lys Lys Ser Gly 
            180                 185                 190         


Leu Arg Gly Arg Gly Gly Gly Gly Phe Pro Thr Gly Val Lys Trp Glu 
        195                 200                 205             


Met Thr Lys Lys Ser Glu Ser Asp Thr Lys Phe Met Ile Cys Asn Ala 
    210                 215                 220                 


Asp Glu Gly Asp Pro Gly Ala Phe Met Asp Arg Ser Ile Leu Glu Gly 
225                 230                 235                 240 


Asp Pro Asn Ser Val Leu Glu Ala Met Ala Ile Ala Gly Tyr Cys Ile 
                245                 250                 255     


Gly Ala Asn Lys Gly Tyr Ile Tyr Ile Arg Ala Glu Tyr Pro Leu Ala 
            260                 265                 270         


Ile Asn Arg Leu Lys Ile Ala Leu Lys Gln Ala Tyr Asp Leu Gly Leu 
        275                 280                 285             


Leu Gly Asp Asn Ile Leu Gly Thr Asp Phe Ser Phe His Ile Asp Leu 
    290                 295                 300                 


Lys Tyr Gly Ala Gly Ala Phe Ile Cys Gly Glu Glu Thr Ala Leu Ile 
305                 310                 315                 320 


Asn Ser Ile Glu Gly Gly Arg Gly Glu Pro Thr Val Lys Pro Pro Phe 
                325                 330                 335     


Pro Ser Gln Ile Gly Leu Trp Lys Lys Pro Thr Asn Ile Asn Asn Val 
            340                 345                 350         


Glu Thr Leu Ala Asn Ile Pro Pro Ile Ile Leu Lys Gly Ser Lys Trp 
        355                 360                 365             


Phe Ser Ser Ile Gly Thr Glu Lys Ser Lys Gly Thr Lys Val Phe Ala 
    370                 375                 380                 


Leu Ala Gly Lys Ile Asn Asn Val Gly Leu Val Glu Val Pro Met Gly 
385                 390                 395                 400 


Ile Thr Leu Arg Glu Ile Ile Tyr Asn Leu Gly Gly Gly Ile Arg Gly 
                405                 410                 415     


Gly Lys Lys Leu Lys Ala Val Gln Thr Gly Gly Pro Ser Gly Gly Cys 
            420                 425                 430         


Ile Pro Ala Asp His Leu Asp Thr Ala Ile Asp Tyr Glu Ser Leu Thr 
        435                 440                 445             


Glu Ile Gly Ser Met Met Gly Ser Gly Gly Met Ile Val Met Asp Glu 
    450                 455                 460                 


Asp Asn Cys Met Val Asn Ile Ala Lys Phe Tyr Leu Gln Phe Ser Val 
465                 470                 475                 480 


Asp Glu Ser Cys Gly Lys Cys Thr Ala Cys Arg Ile Gly Asn Lys Arg 
                485                 490                 495     


Leu Leu Glu Ile Leu Glu Asp Ile Thr Lys Gly Lys Gly Thr Met Glu 
            500                 505                 510         


His Leu Glu Gly Leu Lys Asp Leu Ser Tyr Val Ile Lys Asp Ser Ala 
        515                 520                 525             


Leu Cys Gly Leu Gly Gln Thr Ser Pro Asn Pro Ile Ile Ser Thr Met 
    530                 535                 540                 


Lys Phe Phe Trp Asp Glu Tyr Val Ala His Val Lys Asp Lys Arg Cys 
545                 550                 555                 560 


Pro Ala Gly Val Cys Thr Ala Leu Leu Lys Tyr Asn Ile Asn Ser Glu 
                565                 570                 575     


Lys Cys Ile Gly Cys Thr Ala Cys Thr Lys Val Cys Pro Lys Gly Ala 
            580                 585                 590         


Ile Ser Gly Glu Ile Lys Lys Ser His Val Ile Asp Lys Ser Lys Cys 
        595                 600                 605             


Ile Asn Cys Gly Ala Cys Ser Ser Ile Cys Lys Phe Ser Ala Ile Thr 
    610                 615                 620                 


Lys Glu 
625     


<210>  28
<211>  1881
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_3570, NADH dehydrogenase (quinone) (EC:1.6.99.5)

<400>  28
atggataaga taaaatcctt tgaagattta aaaactttaa gagaaaagta taaagctaag       60

atagcaaacc gtacttatga taatgcagat aaaaatataa aaaaaacttt acttgtatgc      120

ggtggaacag gatgtcgtgc ttcaagaagc ttagatatag tcaacatact tgaaactgaa      180

attaaaaacg caggtctaga aaatacagtt gatgtcattt ctacaggatg ttttgggttt      240

tgtgagaaag gacctatcgt taaagttgta ccagataatg ttttttatgt tgaagttaat      300

accgaaagag caaagctaat tgtgtatgaa catatggcca aagatacagt agttgaggaa      360

gctttatata aagaccctat tactaatgag aaaatatcca accaaacgga tattccattc      420

tataaaaatc aaaaaagaat tgctcttaga aattgcggtc ttttaaaccc tgaagatatt      480

acagaataca tagcactgaa tggctacgaa gctttaggca aagttttaac acaaatgaca      540

cctgacagcg taattgatga aattaaaaaa agtggcttaa gaggcagagg tggcggtggc      600

tttcctactg gcgtaaaatg ggaaatgaca aaaaaatctg aatctgatac aaagtttatg      660

atctgcaatg ctgatgaggg tgatcccggt gcctttatgg atagaagcat acttgaggga      720

gatccaaatt ctgtacttga agctatggct attgcaggtt actgcatagg tgcaaataag      780

ggttatattt atatcagagc tgaatatcct cttgcaataa acagattaaa aattgcttta      840

aagcaagctt acgatttagg tttattgggt gataatattt taggtactga tttttccttt      900

cacatagatt taaaatatgg tgccggagct ttcatctgtg gtgaggaaac tgcactcata      960

aattccatag aaggcggacg tggagagcct accgtaaaac ctccttttcc ttcccaaata     1020

ggtctctgga aaaaaccaac taatataaat aatgtagaaa ctctggcaaa catcccccct     1080

attatattaa aaggctctaa gtggtttagt tctataggaa ctgaaaagag taaaggaacc     1140

aaagtttttg ccttagcagg caagatcaat aatgttggcc ttgttgaggt acctatgggt     1200

ataaccttgc gggaaataat atataattta ggcggaggta ttcgcggtgg taaaaaactt     1260

aaggctgttc aaactggcgg tccttctggc gggtgcattc ctgcagatca tttagatact     1320

gccattgatt acgaaagtct tactgaaata ggctccatga tgggttctgg tggaatgata     1380

gttatggatg aagataattg tatggtgaat atagccaaat tctatctcca atttagtgta     1440

gatgaatcct gtggaaagtg cactgcctgc agaatcggaa ataaaagact tttagaaatt     1500

ttagaggata tcactaaagg aaaaggtacc atggaacatc ttgaaggatt aaaagattta     1560

tcctatgtaa taaaggattc agccctatgt ggtcttggtc aaacatcacc taatccaatt     1620

ataagtacaa tgaaattttt ctgggatgaa tatgtagccc acgtaaaaga taaacgctgt     1680

cctgctggag tttgcactgc acttttaaaa tacaatataa attctgaaaa atgtattggc     1740

tgcacagcct gtacaaaggt atgccctaaa ggagctattt ccggagaaat aaaaaagtca     1800

catgtaatag ataagtcaaa atgtataaat tgtggtgcat gtagtagtat ttgtaagttt     1860

tctgctatta cgaaagaata a                                               1881


<210>  29
<211>  160
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_3571, NADH dehydrogenase (ubiquinone) 24 kDa subunit

<400>  29

Met Asp Val Cys Lys Leu Asp Asn Glu Lys Leu Lys Glu Leu Ser Ser 
1               5                   10                  15      


Tyr Ile Asp Ser Leu Glu Glu Lys Glu Gly Ser Leu Ile Ser Val Leu 
            20                  25                  30          


His Arg Ala Gln Asp Ile Phe Gly Tyr Leu Pro Glu Glu Leu Gln Thr 
        35                  40                  45              


Phe Ile Ala Asn Lys Leu Asp Ile Ser Ala Ala Lys Val Phe Gly Val 
    50                  55                  60                  


Val Thr Phe Tyr Ser Tyr Phe Thr Met Lys Pro Lys Gly Lys His Val 
65                  70                  75                  80  


Ile Ser Ile Cys Met Gly Thr Ala Cys Phe Val Lys Gly Ala Glu Asn 
                85                  90                  95      


Ile Leu Glu Glu Phe Arg Asn Gln Leu Lys Val Lys Asp Gly Phe Thr 
            100                 105                 110         


Thr Glu Asp Gly Leu Phe Thr Ile Asp Ile Leu Arg Cys Val Gly Ala 
        115                 120                 125             


Cys Gly Leu Ala Pro Val Val Val Val Asp Gly Thr Val His Gly Lys 
    130                 135                 140                 


Val Lys Val Glu Asp Val Lys Gly Ile Leu Ser Gln Tyr Thr Leu Lys 
145                 150                 155                 160 


<210>  30
<211>  483
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_3571, NADH dehydrogenase (ubiquinone) 24 kDa subunit

<400>  30
atggatgtat gcaaattaga taacgaaaaa ctaaaagaac tatcttccta tatagatagt       60

ttggaagaaa aagaaggttc acttataagt gtacttcaca gagctcagga tatatttgga      120

taccttcctg aagaattaca aacatttatt gcaaataaac ttgacattag tgcagcaaaa      180

gtatttggcg tagttacttt ctattcatac tttacaatga agcccaaagg taaacatgta      240

ataagcatat gcatgggtac agcttgtttt gttaagggtg cagaaaacat tttagaagaa      300

tttagaaatc agcttaaagt aaaagatgga tttaccacag aagacggatt gttcactata      360

gatattttaa gatgtgttgg agcttgcggc cttgcaccag tagttgtagt tgatggaaca      420

gtccatggga aagtaaaggt cgaagatgtt aaaggaatat taagtcaata taccttaaaa      480

taa                                                                    483


<210>  31
<211>  448
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_0110, hydrogenase large subunit domain protein, 
       WP_013238683.1

<400>  31

Met Arg Asp Asp Tyr Arg Asn Leu Phe Lys Phe Ile Ile Lys Ala Tyr 
1               5                   10                  15      


Tyr Ser Gly Asn Phe Glu Glu Glu Val Met Ser Phe Leu Leu Glu Ser 
            20                  25                  30          


Lys Met Asp Lys Gln Glu Leu Cys Lys Ile Ile Ser Thr Leu Cys Gly 
        35                  40                  45              


Thr Asn Ile Asp Tyr Ser Ser Asn Phe Ile Glu Asn Leu Lys Lys Ala 
    50                  55                  60                  


Ile Lys Ser Tyr Lys Gln Glu Gly Lys Val Val Asn Lys Val Arg Asp 
65                  70                  75                  80  


Cys Ser Met Glu Cys Val Asp Glu Lys Gly Glu Ile Leu Cys Gln Lys 
                85                  90                  95      


Thr Cys Pro Phe Asp Ala Ile Phe Ile Asp Asn Lys Lys Asn Cys Ala 
            100                 105                 110         


Tyr Ile Asp Lys Glu Lys Cys Thr Asp Cys Gly Phe Cys Val Asp Val 
        115                 120                 125             


Cys Pro Thr Gly Gly Ile Met Asp Lys Val Gln Phe Ile Pro Leu Ala 
    130                 135                 140                 


Asp Ile Leu Lys Ser Lys Ser Pro Val Val Ala Ile Val Ala Pro Ala 
145                 150                 155                 160 


Ile Ile Gly Gln Phe Gly Glu Asp Val Thr Met Asp Gln Leu Arg Thr 
                165                 170                 175     


Ala Phe Lys Lys Leu Gly Phe Thr Asp Met Ile Glu Val Ala Phe Phe 
            180                 185                 190         


Ala Asp Met Leu Thr Leu Lys Glu Ser Ile Glu Phe Asp Asn His Val 
        195                 200                 205             


Lys Asp Glu Lys Asp Phe Met Ile Thr Ser Cys Cys Cys Pro Met Trp 
    210                 215                 220                 


Val Ala Met Val Lys Lys Val Tyr Ser Asn Leu Val Lys His Val Ser 
225                 230                 235                 240 


Pro Ser Val Ser Pro Met Val Ala Gly Gly Arg Val Leu Lys Lys Leu 
                245                 250                 255     


Asn Pro Tyr Cys Lys Val Val Phe Ile Gly Pro Cys Ile Ala Lys Lys 
            260                 265                 270         


Ser Glu Ala Lys Glu Glu Asp Ile Lys Gly Ala Ile Asp Phe Val Leu 
        275                 280                 285             


Thr Phe Glu Glu Leu Arg Asp Ile Phe Asp Ala Phe His Ile Val Pro 
    290                 295                 300                 


Ser Lys Leu Glu Gly Asp Phe Ser Ser Lys Tyr Ala Ser Arg Gly Gly 
305                 310                 315                 320 


Arg Leu Tyr Ala Arg Thr Gly Gly Val Ser Ile Ala Val Ser Glu Ala 
                325                 330                 335     


Val Glu Arg Ile Phe Pro Glu Lys His Lys Leu Phe Ser Ala Ile Gln 
            340                 345                 350         


Ala Asn Gly Ile Arg Glu Cys Arg Glu Met Leu Thr Lys Val Gln Asn 
        355                 360                 365             


Gly Glu Ile Lys Ala Asn Phe Ile Glu Gly Met Gly Cys Ile Gly Gly 
    370                 375                 380                 


Cys Val Gly Gly Pro Lys Ala Ile Val Leu Lys Asp Glu Gly Arg Asp 
385                 390                 395                 400 


Arg Val Asn Lys Phe Ala Gln Asp Ser Glu Ile Lys Val Ala Val Asp 
                405                 410                 415     


Ser Glu Cys Met His Gly Val Leu His Ala Leu Asp Ile His Ser Ile 
            420                 425                 430         


Asp Asp Phe Lys Asp Glu Lys Lys Ile Glu Leu Leu Glu Arg Glu Phe 
        435                 440                 445             


<210>  32
<211>  1347
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_0110, hydrogenase large subunit domain protein

<400>  32
ttgagagatg attataggaa tctatttaaa ttcataataa aggcatatta tagtggaaat       60

tttgaagaag aagtgatgtc atttttatta gagtctaaaa tggataaaca ggaattgtgt      120

aagattatat ctacgttgtg cggcactaat atagattata gttccaattt tatagaaaat      180

ttgaaaaaag caataaagtc ttataaacaa gaaggtaaag tagtcaataa agttagagac      240

tgttccatgg aatgtgtgga tgaaaaaggt gagatacttt gtcaaaaaac atgtcctttt      300

gatgcaattt ttatagacaa taagaaaaat tgtgcttaca tagataaaga aaaatgtacc      360

gattgtggtt tttgtgtaga tgtttgtcct actgggggaa taatggataa agttcagttc      420

attcctcttg ctgatatttt aaaaagcaaa tctccagttg tggctatagt ggctcctgcc      480

ataataggac agtttggcga agatgttact atggatcaac ttaggactgc ttttaaaaaa      540

ctgggattta ctgatatgat tgaagtggca ttttttgcag atatgcttac tttaaaggag      600

tctattgaat ttgacaatca tgtaaaagat gaaaaagatt ttatgataac ctcctgctgc      660

tgccctatgt gggtagctat ggtaaaaaag gtatacagta atttggttaa acacgtatcc      720

ccctctgtat ctccaatggt tgcaggagga agagtactta aaaagttaaa cccttactgc      780

aaggtagtgt ttataggacc atgtattgct aaaaaatctg aggcaaagga agaagacata      840

aaaggagcaa tagatttcgt acttactttt gaagaattaa gagatatatt tgatgctttt      900

catatagttc catctaaact tgaaggagat ttttcctcta aatatgcgtc taggggtgga      960

agattatatg ctcgtacagg gggagtttct attgcagtaa gcgaagctgt ggaaagaatt     1020

ttccctgaaa agcataaact atttagtgca attcaggcaa atggcattag agaatgtaga     1080

gaaatgctta ccaaggtgca aaatggagaa ataaaagcta attttattga aggaatgggc     1140

tgcattggcg gatgtgtagg tggtcccaaa gcaattgtac ttaaggatga aggtagggat     1200

cgagtaaata aatttgcaca agattctgaa ataaaagttg ctgtagatag tgaatgcatg     1260

catggagtat tacatgcttt ggatatacat tctatagatg attttaagga tgagaaaaaa     1320

atagaactgt tagaacgaga attttaa                                         1347


<210>  33
<211>  457
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_3841, hydrogenase, Fe-only (EC:1.12.7.2), WP_035787664

<400>  33

Met Thr Val Lys Ser Glu Gly Ile Val Lys Ile Asp Lys Glu Leu Cys 
1               5                   10                  15      


Thr Gly Cys Arg Arg Cys Ala Asp Ile Cys Pro Val Asp Ala Ile Glu 
            20                  25                  30          


Gly Glu Lys Gly Gln Pro Gln Thr Ile Asn Thr Glu Arg Cys Val Leu 
        35                  40                  45              


Cys Gly Gln Cys Val Gln Ile Cys Ser Ala Tyr Ala Ser Ala Phe Asp 
    50                  55                  60                  


Glu Asp Ile Thr Pro His Lys Glu Lys Ile Lys Glu Arg Asn Met Leu 
65                  70                  75                  80  


Pro Ser Val Lys Glu Pro Leu Phe Ala Ser Tyr Tyr Arg Gly Asp Ala 
                85                  90                  95      


Pro Ala Val Lys Glu Ala Leu Ala Asn Ser Lys Leu Phe Thr Met Val 
            100                 105                 110         


Gln Cys Ala Pro Ala Val Arg Val Ala Ile Ala Glu Glu Phe Gly Met 
        115                 120                 125             


Pro Leu Gly Ser Leu Thr Pro Gly Lys Met Ala Ala Ala Leu Arg Glu 
    130                 135                 140                 


Leu Gly Phe Asp Arg Ile Tyr Asp Thr Asn Phe Ala Ala Asp Leu Thr 
145                 150                 155                 160 


Ile Met Glu Glu Gly Ser Glu Leu Ile Lys Arg Val Thr Glu Gly Gly 
                165                 170                 175     


Val Leu Pro Met Phe Thr Ser Cys Cys Pro Ala Trp Val Lys Phe Ile 
            180                 185                 190         


Glu Gln Asp Tyr Pro Glu Leu Ile Pro His Leu Ser Ser Cys Lys Ser 
        195                 200                 205             


Pro Gln Gln Met Glu Gly Ala Leu Leu Lys Thr Tyr Gly Ala Gln Val 
    210                 215                 220                 


Asp Gly Val Asp Ala Gly Lys Ile Tyr Ser Val Ser Val Met Pro Cys 
225                 230                 235                 240 


Ile Cys Lys Lys Phe Glu Cys Glu Arg Pro Glu Met Lys Asp Ser Gly 
                245                 250                 255     


Tyr Gln Asp Val Asp Ala Val Ile Thr Thr Arg Glu Leu Ala Gln Leu 
            260                 265                 270         


Ile Lys Asp Asp Gly Ile Asp Phe Asn Gly Leu Pro Glu Lys Glu Phe 
        275                 280                 285             


Asp Lys Pro Leu Gly Thr Tyr Ser Gly Ala Gly Thr Ile Phe Cys Ala 
    290                 295                 300                 


Thr Gly Gly Val Met Glu Ala Ala Leu Arg Thr Ala Tyr Lys Leu Ile 
305                 310                 315                 320 


Thr Lys Glu Glu Ile Pro Asp Val Asp Leu Lys Phe Ile Arg Gly Gly 
                325                 330                 335     


Glu Gly Ala Arg Ser Ser Glu Ile Lys Val Gly Asp Leu Thr Leu Lys 
            340                 345                 350         


Val Ala Val Val Ala Gly Leu Lys Asn Val Val Pro Val Leu Glu Ala 
        355                 360                 365             


Ile Lys Thr Gly Lys Ala Asp Phe His Phe Ile Glu Val Met Thr Cys 
    370                 375                 380                 


Pro Val Gly Cys Val Ser Gly Gly Gly Gln Pro Lys Val Leu Ile Pro 
385                 390                 395                 400 


Asp Glu Lys Ala Asp Ser Tyr Thr Asn Arg Thr Cys Ser Thr Tyr Val 
                405                 410                 415     


His Asp Glu Asn Met Glu Tyr Arg Lys Ser His Asp Asn Pro Glu Ile 
            420                 425                 430         


Gln Lys Ile Tyr Lys Glu Phe Leu Val Glu Asp Asn Ile His His Leu 
        435                 440                 445             


Leu His Thr Thr Tyr Thr Pro Arg Arg 
    450                 455         


<210>  34
<211>  1374
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_3841, hydrogenase, Fe-only (EC:1.12.7.2)

<400>  34
ttgacagtta aaagtgaagg tattgtcaaa attgataaag agttgtgtac aggatgtaga       60

cgatgtgcag atatttgtcc tgtagatgct atagaaggtg agaagggaca acctcaaaca      120

attaatactg aacgctgtgt tttgtgtggt cagtgtgtac aaatttgcag tgcttatgca      180

tctgcatttg atgaagatat tactcctcat aaggaaaaga taaaagagcg taatatgctt      240

ccatctgtta aagagccctt atttgcatcc tactatagag gagatgctcc agcagtaaaa      300

gaggccttag caaattctaa actttttact atggttcaat gcgcaccagc agtacgtgtg      360

gctattgccg aagaatttgg tatgccactt ggaagtttaa caccagggaa aatggcagct      420

gcgctaagag agttaggttt tgatcgaatt tatgatacta attttgctgc tgatctaact      480

attatggagg aaggcagtga acttattaaa agggttactg aaggtggagt actgccaatg      540

ttcacttcat gttgtcctgc ttgggtaaaa tttattgagc aggattatcc ggaacttatt      600

ccacatctgt cttcttgtaa atctccgcag caaatggaag gtgctctgct taaaacatat      660

ggtgcacagg ttgatggtgt agatgctggc aagatttata gtgtttcagt tatgccttgt      720

atttgcaaaa aatttgaatg tgaacgtcct gaaatgaaag acagtggata tcaggatgta      780

gatgctgtaa ttaccacacg ggaacttgca caattaatca aggatgatgg cattgatttt      840

aatggtttac ctgaaaaaga atttgacaag ccacttggaa cttattctgg tgcaggcact      900

attttctgtg ctactggtgg tgttatggaa gctgccctgc gtacggcata taaattgatt      960

actaaagaag agattccaga tgtcgatctc aaattcataa gaggaggcga aggcgcaaga     1020

agttcagaaa ttaaagtagg agatttaaca ctaaaagtag cagtagttgc tggtctgaaa     1080

aatgttgtac cagttttgga agcaattaaa actgggaaag cagatttcca tttcattgaa     1140

gtgatgacct gtccagttgg atgtgttagc gggggcggac aacctaaggt attgatacct     1200

gatgaaaaag ctgattcgta tactaatcgt acatgcagta cgtatgtaca tgatgaaaat     1260

atggaatata gaaaatcaca tgataatcct gaaatacaga aaatttataa agaattcttg     1320

gtagaagata atattcatca tttgcttcat actacgtata cgccaaggag gtaa           1374


<210>  35
<211>  446
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_0119, hydrogenase large subunit domain protein

<400>  35

Met Asn Lys Ser Pro Val Thr Val Leu Lys Glu Lys Cys Thr Gly Cys 
1               5                   10                  15      


Asn Lys Cys Ile Arg Thr Cys Pro Ile Leu Gly Ala Asn Val Thr Ala 
            20                  25                  30          


Thr Glu Asn Gly Val Ser Lys Val Tyr Ile Asp Glu Glu Arg Cys Ile 
        35                  40                  45              


Gly Cys Gly Glu Cys Val Lys Val Cys Glu His Gly Ala Arg Asp Phe 
    50                  55                  60                  


Asn Asp Ser Thr Gln Asp Phe Phe Lys Asp Leu Lys Lys Gly Lys Lys 
65                  70                  75                  80  


Ile Thr Val Ile Ala Ala Pro Ser Ile Ile Val Asn Ile Lys Asn Tyr 
                85                  90                  95      


Lys Lys Phe Phe Gly Tyr Leu Lys Ser Leu Gly Val Ser Ile Ile Tyr 
            100                 105                 110         


Asp Val Ser Phe Gly Ala Asp Ile Thr Thr Trp Ala Tyr Leu Lys Ala 
        115                 120                 125             


Met Lys Glu Lys Asn Ile Ser Ser Leu Ile Ser Gln Pro Cys Pro Ile 
    130                 135                 140                 


Val Val Asn Tyr Ile Glu Lys Tyr Lys Pro Glu Leu Ile Glu Tyr Leu 
145                 150                 155                 160 


Ala Pro Ile His Ser Pro Met Met Cys Thr Ala Val Tyr Leu Lys Lys 
                165                 170                 175     


Tyr Lys His Ile Cys Glu Asp Ile Ala Phe Leu Ser Pro Cys Ile Gly 
            180                 185                 190         


Lys Leu Ile Glu Ile Asn Asp Lys Asn Thr Asp Gly Tyr Val Lys Tyr 
        195                 200                 205             


Asn Val Thr Tyr Lys Lys Ile Leu Asp Tyr Leu Arg Asp Asn Asn Val 
    210                 215                 220                 


Asn Leu Asn Asn Tyr Asp Glu Val Glu Phe Asp Asn Val Pro Ala Ser 
225                 230                 235                 240 


Leu Gly Val Val Tyr Ser Leu Pro Gly Gly Leu Lys Ala Asn Val Lys 
                245                 250                 255     


Ala Arg Thr Glu Glu Leu His Val Leu Gln Ile Glu Gly His Lys Glu 
            260                 265                 270         


Ala Ile Glu Tyr Leu Asn Lys Tyr Ser Asp Arg Val Lys Ala Asn Lys 
        275                 280                 285             


Leu Ile Pro Ser Leu Leu Asp Ile Leu Asn Cys Lys Asn Gly Cys Asn 
    290                 295                 300                 


Ile Gly Thr Ala Ser Leu Asp Asn Leu Thr Glu Tyr Asp Ile Gln Tyr 
305                 310                 315                 320 


Arg Phe His Asp Ile Lys Val Glu Lys Leu Arg Glu Lys Thr Gly Leu 
                325                 330                 335     


Phe Lys Lys Lys Ile Lys Ser Ile Asp Glu Tyr Phe Asp Lys Asn Leu 
            340                 345                 350         


Asn Leu Asn Asp Phe Val Arg Lys Tyr Thr Ala Gln Lys Val Lys Lys 
        355                 360                 365             


Ile Ile Glu Pro Thr Gln Lys Asp Tyr Asp Asn Ile Phe Asp Glu Met 
    370                 375                 380                 


Met Lys Thr Thr Thr Leu Gly Lys Glu Phe Asn Cys Ser Ala Cys Gly 
385                 390                 395                 400 


Tyr Ser Thr Cys Lys Glu Met Val Lys Met Ile Phe Asn Gly Ile Asn 
                405                 410                 415     


Ser Lys Glu Asn Cys Ile Tyr Tyr Val Lys Lys Lys Ser Ile Trp Asn 
            420                 425                 430         


Ile Ala Asn Trp Lys Lys Lys Met Lys Arg Ser Lys Ser Leu 
        435                 440                 445     


<210>  36
<211>  1341
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_0119, hydrogenase large subunit domain protein

<400>  36
gtgaataaaa gtcctgtaac tgttttaaaa gaaaaatgta caggttgtaa taagtgtata       60

agaacttgtc ctattcttgg tgcaaatgtt acagcaactg aaaatggtgt aagtaaggtt      120

tacatagatg aggaaagatg tattggatgc ggtgaatgtg taaaggtttg cgaacatggg      180

gctagagact ttaacgatag tacacaagac ttttttaagg atttgaaaaa aggcaaaaaa      240

ataacagtaa tagctgcacc atctattata gttaatatta aaaattacaa gaaatttttt      300

ggatatctta aatctttggg agtatctata atttatgatg tttctttcgg tgcagatatt      360

actacctggg cttatctaaa agcaatgaag gagaaaaata tttcttcttt aatatcacag      420

ccctgtccta tcgtggttaa ttatattgaa aaatataagc ctgaattaat agaatattta      480

gcgcctatac acagtcctat gatgtgtact gctgtttatc tgaaaaaata taagcatata      540

tgtgaagata tagcttttct gtcaccttgt attggcaagc taattgaaat caatgataag      600

aatacagatg ggtatgtgaa atataatgta acgtacaaaa agattttaga ttatttgaga      660

gataataatg tgaatttgaa caattacgat gaagttgaat ttgataatgt tcctgcttct      720

ttgggggttg tttatagttt accaggtgga ttaaaagcaa atgtaaaagc tagaactgaa      780

gaactacatg ttcttcagat agaaggacac aaagaggcaa ttgagtactt gaataagtat      840

tctgatagag ttaaagctaa taaacttata cctagtttac ttgatatttt aaattgcaaa      900

aatggatgta atataggtac agcttcctta gacaatttaa cggaatatga tattcaatat      960

aggtttcatg atataaaggt ggaaaagtta agagaaaaaa ctggcttgtt taagaaaaaa     1020

atcaaatcaa tagacgagta ctttgataaa aatcttaatt taaacgattt tgtaagaaag     1080

tatactgcac agaaggttaa aaaaataatt gaacctaccc aaaaagatta tgacaacata     1140

tttgatgaga tgatgaaaac tacaacattg ggaaaagaat tcaattgttc cgcttgtgga     1200

tacagcactt gcaaagaaat ggtaaagatg atttttaacg gcataaattc taaggaaaat     1260

tgtatttatt atgtgaagaa aaaatcaata tggaatatag cgaactggaa gaaaaaaatg     1320

aagaggtcaa agagtctata a                                               1341


<210>  37
<211>  461
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_0861, Cytochrome-c3 hydrogenase (EC:1.12.2.1)

<400>  37

Met Lys Lys Lys Ile Thr Ile Asp Pro Ile Thr Arg Ile Ser Gly Phe 
1               5                   10                  15      


Leu Glu Thr Lys Val Gln Val Glu Lys Asn Ile Ile Val Asp Ala Glu 
            20                  25                  30          


Thr Ser Gly Leu Leu Phe Arg Gly Phe Glu Lys Met Leu Lys Asn Arg 
        35                  40                  45              


Glu Pro Leu Asp Ala Val Tyr Phe Thr Glu Arg Ile Cys Gly Ile Cys 
    50                  55                  60                  


Ser Thr Ala His Ala Val Ala Ala Ala Thr Ala Leu Glu Asp Ala Leu 
65                  70                  75                  80  


Lys Ile Lys Ile Ser Val Asn Asp Ser Tyr Met Arg Asn Leu Ile His 
                85                  90                  95      


Gly Phe Glu Phe Ile Gln Asn His Ile Arg His Phe Tyr Asn Leu Thr 
            100                 105                 110         


Ile Pro Ser Tyr Val Lys Met Pro Asp Ile Asn Pro Leu Ser Ser Asn 
        115                 120                 125             


Gln Tyr Glu Asp Tyr Arg Leu Pro Tyr Asn Leu Asn Lys Lys Ile Ser 
    130                 135                 140                 


Glu Asp Tyr Ile Glu Ser Ile Lys Tyr Ser Arg Leu Ala His Glu Gly 
145                 150                 155                 160 


Leu Ala Ile Leu Gly Gly Lys Ala Pro His Asn His Gly Ile Phe Val 
                165                 170                 175     


Gly Gly Val Thr Ile Asn Ile Asp Pro Tyr Lys Leu Thr Lys Val Lys 
            180                 185                 190         


Ser Ile Ile Ser Gln Ile Asn Lys Phe Val Ser Ser Val Met Leu Glu 
        195                 200                 205             


Asp Met Asn Ile Ile Ser Lys Tyr Tyr Ala Asp Tyr Phe Lys Met Gly 
    210                 215                 220                 


Gly Ala Tyr Gly Asn Phe Met Thr Tyr Gly Ile Phe Asp Lys Tyr Ala 
225                 230                 235                 240 


Asp Pro Glu Ile Ser Tyr Val Gly Pro Ser Val Leu Ile Asn Gly Arg 
                245                 250                 255     


Lys Tyr Asn Phe Asn Ser Asn Lys Ile Thr Glu Asn Ile Leu His Thr 
            260                 265                 270         


Trp Tyr Thr Ser Asp Asp Glu Thr Ile Asn Leu Ser Lys Glu Thr Gly 
        275                 280                 285             


Tyr Ser Phe Ile Lys Ser Pro Thr Tyr Asn Gly Tyr Ser Met Glu Val 
    290                 295                 300                 


Gly Pro Leu Ala Arg Leu Ile Leu Ser Gly Glu Tyr Thr Gly Gly Ser 
305                 310                 315                 320 


Ser Cys Met Asp Arg Asn Val Ala Arg Val Leu Glu Thr Lys Lys Ile 
                325                 330                 335     


Leu Glu Ile Met Gln Gly Leu Ala Asp Arg Ile Lys Leu Ile Pro Ala 
            340                 345                 350         


Glu Gln Arg Ile Tyr Gln Ile Pro Asp Lys Ala Phe Gly Ala Gly Leu 
        355                 360                 365             


Ile Asp Thr Thr Arg Gly Ser Leu Gly His Trp Ile Ser Ile Glu Asp 
    370                 375                 380                 


Lys Phe Ile Lys His Tyr Asn Ile Ile Thr Pro Thr Val Trp Asn Met 
385                 390                 395                 400 


Gly Pro Arg Asn Gln Ser Gly Ala Leu Gly Ile Gly Glu Lys Ser Leu 
                405                 410                 415     


Val Gly Thr Lys Ile Lys Asp Ile Lys Gln Pro Ile Glu Val Gly Arg 
            420                 425                 430         


Ile Met Arg Ser Phe Asp Pro Cys Val Ser Cys Ala Thr His Leu Val 
        435                 440                 445             


Ser Asp Lys Tyr Glu Pro Val Asp Val Gln Val Ile Val 
    450                 455                 460     


<210>  38
<211>  1386
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_0861, Cytochrome-c3 hydrogenase (EC:1.12.2.1)

<400>  38
atgaaaaaga aaattacaat tgatccaatt acgagaataa gcggtttttt ggaaactaaa       60

gtgcaagtag aaaaaaatat tatagtagat gctgaaacta gtggattgct ttttagagga      120

tttgaaaaaa tgttaaagaa cagagagccg ctggatgcag tatattttac agaaagaatt      180

tgtggaatat gttcaacagc tcatgccgtg gcggctgcta cagctcttga agatgctttg      240

aagataaaaa ttagtgtaaa tgattcgtat atgcgtaatt taatacatgg ttttgaattt      300

atacaaaatc atataagaca tttttataat ttgaccatac caagttatgt gaagatgccc      360

gatataaatc ctctttcttc aaatcaatat gaagattata gattgcctta taacctaaat      420

aaaaagataa gtgaagatta tattgaaagt attaaataca gcaggttagc ccatgaaggg      480

ttggctatcc ttggaggaaa agccccccat aatcatggaa tttttgttgg aggagttacc      540

ataaatatag atccatataa actcacaaaa gttaaatcta ttatttctca aattaataaa      600

tttgtaagta gtgttatgtt agaggacatg aacataattt caaaatacta tgctgattat      660

tttaaaatgg gaggagctta tggaaacttt atgacttatg gaatttttga caagtatgct      720

gatcctgaga taagttatgt aggaccttct gtattaataa atggacgaaa gtataatttc      780

aatagtaata aaattacgga aaacatactc cacacctggt atacaagcga tgatgaaacg      840

ataaatttat ctaaagaaac aggttacagc tttataaaat cgccaaccta taatggatat      900

tctatggaag taggacctct agcaagattg atactttcag gtgagtatac tggtggaagt      960

tcatgtatgg acagaaatgt tgccagagta cttgaaacaa aaaagatttt agaaattatg     1020

caaggacttg cagatagaat taagcttatt ccagcagaac aaagaatata tcaaatacca     1080

gataaagcat ttggtgcagg attaattgac acaactagag gatccttggg acactggata     1140

agtatagaag ataaatttat aaagcattac aatattataa ctcctacagt gtggaatatg     1200

gggccaagaa atcaatcagg tgcgcttgga attggagaaa aatctttagt tggaacgaaa     1260

ataaaagata taaagcagcc tatagaagtt gggagaatta tgagatcttt tgatccttgt     1320

gtttcctgtg caacgcatct tgtaagtgat aaatatgaac cagtggatgt acaggttata     1380

gtatga                                                                1386


<210>  39
<211>  250
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_0862, hydrogenase (NiFe) small subunit HydA (EC:1.12.99.6)

<400>  39

Met Asn Ala Arg Ser Lys Val Ile Cys Pro Leu Ile Val Asp Lys Glu 
1               5                   10                  15      


Arg Ser Ser Lys Ala Phe Thr Ser Glu Ala Ile Asp Leu Ile Glu Arg 
            20                  25                  30          


Arg Lys Thr Lys Lys Leu Asn Ala Ile Trp Leu Glu Val Thr Gly Cys 
        35                  40                  45              


Ser Gly Asn Ile Ile Ser Phe Leu Asn Ser Glu Asn Pro Gly Leu Asp 
    50                  55                  60                  


Tyr Ile Leu Glu Lys Leu Ile Asn Leu Lys Tyr Asn Asn Thr Leu Met 
65                  70                  75                  80  


Thr Ser Glu Gly Glu Tyr Ala Phe Lys Gln Phe Leu Asp Thr Leu Asn 
                85                  90                  95      


Thr Glu Phe Ile Leu Leu Val Asp Gly Ala Val Ser Thr Ala Gln Asn 
            100                 105                 110         


Gly Phe Tyr Asn Ile Val Ala Asn Tyr Glu Gly Asn Pro Val Thr Ala 
        115                 120                 125             


Leu Glu Ala Val Lys Met Ala Gly Glu Lys Ala Lys His Val Leu Cys 
    130                 135                 140                 


Val Gly Thr Cys Ala Ser Tyr Gly Gly Ile Ser Ala Ala Arg Pro Asn 
145                 150                 155                 160 


Pro Ser Glu Ser Lys Ser Val Lys Glu Ile Leu Asn Arg Glu Val Ile 
                165                 170                 175     


Arg Leu Pro Gly Cys Pro Cys His Pro Asp Trp Val Val Gly Thr Leu 
            180                 185                 190         


Ala His Leu Val Ala Phe Gly Lys Pro Gln Leu Asp Lys Glu Gly Arg 
        195                 200                 205             


Pro Leu Leu Phe Tyr Gly Ile Thr Ile His Asp Ser Cys Thr Arg Arg 
    210                 215                 220                 


Gly Phe Phe Asp Asn Arg Ile Phe Ala Lys Lys Phe Gly Glu Asn Gly 
225                 230                 235                 240 


Cys Met Phe Lys Leu Gly Cys Arg Gly Leu 
                245                 250 


<210>  40
<211>  753
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_0862, hydrogenase (NiFe) small subunit HydA (EC:1.12.99.6)

<400>  40
atgaatgctc gaagcaaggt tatatgtcct ttaatcgtag ataaggaacg cagttcaaag       60

gcttttacta gtgaagctat agatttaatt gaaaggagaa agacgaaaaa attaaatgct      120

atatggcttg aagtaacagg atgttcagga aatatcattt cttttttaaa tagtgaaaat      180

cctggactcg attatatttt ggaaaaactc attaatttaa aatacaacaa tactctaatg      240

acttcagaag gggagtatgc ctttaaacaa ttcttagata cattgaatac tgaatttata      300

ctattagtag atggagcagt atctactgcc cagaacggtt tttataatat tgttgccaat      360

tatgaaggaa accctgttac tgcacttgaa gctgtaaaaa tggcaggaga aaaagcaaag      420

catgttctct gtgtaggaac ttgtgcatcc tatggtggaa tttctgccgc caggccaaac      480

ccttcagaaa gcaaaagtgt taaagaaata ctaaatcgtg aagtcataag acttccaggc      540

tgtccatgcc acccggattg ggtagttgga actttagcac acttggttgc ttttggaaaa      600

ccacaattgg ataaagaagg aagacctctt cttttttatg gaattaccat tcatgatagt      660

tgtacaagaa gaggattttt tgataacaga atttttgcaa aaaaatttgg agaaaatgga      720

tgcatgttta aacttggatg caggggcctg taa                                   753


<210>  41
<211>  154
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_0860, hydrogenase maturation protease (EC:3.4.23.-), 
       WP_013239503.1

<400>  41

Met Lys Ala Lys Val Ile Ala Leu Gly Asn Ile Leu Met Glu Asp Asp 
1               5                   10                  15      


Gly Ile Gly Ile Arg Ile Leu Glu Asn Ile Lys Glu Glu Leu Thr His 
            20                  25                  30          


Asn Asn Val Glu Ser Ile Ser Gly Glu Thr Asp Val Glu Tyr Cys Ile 
        35                  40                  45              


Ser Gln Val Lys Asp Gly Asp Phe Ile Phe Ile Ile Asp Ala Ser Tyr 
    50                  55                  60                  


Asn Gly Lys Val Pro Gly Thr Ile Thr Val Ala Ser Leu Gln Asp Tyr 
65                  70                  75                  80  


Lys Cys Lys Lys Lys Tyr Tyr Thr Gln His Ser Tyr Ser Phe Ile Asp 
                85                  90                  95      


Leu Ile Gly Val Tyr Tyr Lys Ser Leu Thr Gly Phe Ile Ile Glu Ile 
            100                 105                 110         


Glu Ala Ala Ser Ile Ser Phe Lys Leu Gly Leu Ser His Asn Leu Gln 
        115                 120                 125             


Asn Lys Leu Lys Ser Ile Ser Lys Asp Val Leu Lys Asn Ile Phe Leu 
    130                 135                 140                 


Arg Leu Asn Asp Arg Ala Trp Glu Glu Lys 
145                 150                 


<210>  42
<211>  465
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_0860, hydrogenase maturation protease (EC:3.4.23.-)

<400>  42
atgaaagcaa aagttattgc tctaggaaac atattgatgg aggatgatgg cattggaatt       60

agaatactgg aaaatataaa ggaggagctt acgcataaca atgttgaatc tataagtgga      120

gagacagatg tggaatattg catttcccaa gtaaaagatg gtgattttat atttataata      180

gatgcttctt ataatggaaa agttccaggt acgataacag ttgccagctt acaagattat      240

aagtgtaaaa agaaatatta tactcagcat agctatagtt tcatagattt gataggagtt      300

tattacaaat ccttaactgg atttattatt gaaattgaag cagctagtat aagctttaaa      360

ttgggactta gccataattt acagaataag cttaagtcta tttcaaaaga tgtattgaaa      420

aatatttttc tgagattgaa tgatagagca tgggaggaaa aatag                      465


<210>  43
<211>  334
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_0369, hydrogenase expression/formation protein HypE

<400>  43

Met Asp Lys Thr Val Thr Leu Ala His Gly Ala Gly Gly Arg Gln Thr 
1               5                   10                  15      


Ser Glu Leu Ile Asp Gln Val Phe Arg Ala His Phe Ser Asn Pro Asp 
            20                  25                  30          


Leu Thr Ala Asp Asp Ala Ala Val Leu Asn Ile Lys Gly Gly Lys Leu 
        35                  40                  45              


Ala Phe Thr Thr Asp Gly Phe Ile Val Ser Pro Ser Glu Phe Pro Gly 
    50                  55                  60                  


Gly Asn Ile Gly Lys Leu Ser Ile Cys Gly Thr Val Asn Asp Leu Ser 
65                  70                  75                  80  


Cys Met Gly Ala Lys Pro Leu Tyr Leu Ser Cys Ala Phe Val Ile Glu 
                85                  90                  95      


Glu Gly Phe Pro Met Asp Lys Leu Glu Lys Ile Ala Ala Ala Met Glu 
            100                 105                 110         


Lys Thr Ala Lys Glu Ala Gly Val Lys Ile Ala Ala Gly Asp Thr Lys 
        115                 120                 125             


Val Ala Gly Lys Gly Gln Val Asp Gly Val Phe Ile Thr Thr Thr Gly 
    130                 135                 140                 


Ile Gly Gln Ile Met Asp Asp Ala Asn Thr Ser Gly Phe Asn Ala Lys 
145                 150                 155                 160 


Pro Gly Asp Ala Ile Ile Val Thr Gly Asp Ile Gly Arg His Gly Cys 
                165                 170                 175     


Thr Val Leu Leu Ala Arg Asp Glu Phe Gly Ile Glu Ala Asp Val Thr 
            180                 185                 190         


Ser Asp Cys Ala Pro Leu Trp Gly Thr Val Lys Ala Met Phe Asp Thr 
        195                 200                 205             


Ser Lys Asp Ile His Val Ile Arg Asp Ala Thr Arg Gly Gly Val Gly 
    210                 215                 220                 


Thr Val Leu Tyr Glu Ile Ala Glu Gln Ser Lys Val Gly Ile Arg Leu 
225                 230                 235                 240 


Asp Ser Lys Ser Ile Pro Val Ala Asp Gly Val Lys Gly Val Cys Gly 
                245                 250                 255     


Met Leu Gly Leu Glu Pro Leu Tyr Leu Ala Cys Glu Gly Arg Leu Val 
            260                 265                 270         


Val Phe Ala Pro Lys Glu Ile Ala Pro Lys Leu Val Asp Thr Leu His 
        275                 280                 285             


Lys Gly Lys Tyr Ser Lys Asp Ala Ala Ile Ile Gly Glu Val Thr Cys 
    290                 295                 300                 


Asp Met Pro Gly Arg Val Ile Val Lys Thr Glu Ile Gly Ala Glu Thr 
305                 310                 315                 320 


Leu Leu Pro Pro Pro Gly Gly Glu Leu Leu Pro Arg Ile Cys 
                325                 330                 


<210>  44
<211>  1005
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_0369, hydrogenase expression/formation protein HypE

<400>  44
atggataaaa cagttacact agctcatgga gctggaggaa gacaaacatc agaattaata       60

gatcaggttt ttagagctca tttttcgaat cctgacctga cagcagatga tgctgcagta      120

ttgaacatta aaggaggaaa actagctttt acaacagatg gatttattgt ttctccatcg      180

gaatttccag gtggaaatat tggcaaatta agcatttgtg gtacagtaaa tgacttatcc      240

tgtatgggag cgaaacctct gtatttatct tgtgcttttg ttattgaaga gggcttccca      300

atggataaat tagagaaaat tgcagcagcc atggaaaaga ctgcaaagga agcaggggtt      360

aaaatagccg caggagatac aaaggttgct ggaaaagggc aagttgatgg agtatttata      420

acaacaacag gtataggaca gattatggat gatgctaata cctctggttt taatgcaaaa      480

ccaggcgatg cgattattgt aaccggtgat atagggcgac atggatgtac tgttttactg      540

gcacgagatg agtttggaat tgaagcagat gtaaccagtg attgtgcacc attatggggt      600

acagtaaaag ctatgtttga tacgtcaaaa gatattcatg taatcagaga tgctacacgt      660

ggaggtgtcg gtacggtatt atatgaaatt gccgagcaga gcaaagttgg aattagatta      720

gattcaaaga gtataccggt agcagatggt gtaaaaggtg tatgcggcat gctggggttg      780

gaaccactat atcttgcatg tgaaggaaga cttgtagttt ttgctccaaa agaaattgct      840

ccaaaacttg tggatacatt acataaaggt aaatattcaa aagatgcagc cattattggt      900

gaggtcactt gtgatatgcc tggacgtgtt attgtaaaaa cagaaatcgg tgctgaaaca      960

ttgctgccac ctccaggagg agaattgctt ccaagaatat gttaa                     1005


<210>  45
<211>  352
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_0370, hydrogenase expression/formation protein HypD

<400>  45

Met Ala Glu Asn Ile Lys Lys Thr Ala Lys Glu Ile Ile Glu Ser Tyr 
1               5                   10                  15      


Asp Gly Pro Lys Ile Arg Ile Met Glu Val Cys Gly Thr His Thr His 
            20                  25                  30          


Glu Ile Phe Arg Leu Gly Ile Arg Asn Ile Ile Pro Glu Asn Ile Glu 
        35                  40                  45              


Leu Ile Ser Gly Pro Gly Cys Pro Val Cys Val Thr Pro Val Gly Phe 
    50                  55                  60                  


Ile Asp Glu Ala Ile Met Leu Ala Leu Asp His Asn Ala Thr Ile Cys 
65                  70                  75                  80  


Thr Phe Gly Asp Leu Val Arg Val Pro Gly Ser Glu Met Asn Leu Ala 
                85                  90                  95      


Gly Ala Arg Ser Lys Gly Ala Lys Val Gln Ile Val Tyr Ser Pro Ile 
            100                 105                 110         


Asp Ala Tyr Glu Tyr Ala Arg Asp His Leu Asp Glu Gln Val Val Phe 
        115                 120                 125             


Leu Ser Val Gly Phe Glu Thr Thr Thr Pro Ala Ser Cys Leu Ala Val 
    130                 135                 140                 


Lys Lys Ala Lys Gly Ala Glu Leu Lys Asn Phe Ala Leu Leu Thr Ala 
145                 150                 155                 160 


Asn Lys Thr Met Pro Gly Val Tyr Lys Thr Leu Lys Asn Ser Ala Asp 
                165                 170                 175     


Ala Phe Leu Tyr Pro Gly His Val His Ala Ile Thr Gly Thr Glu Leu 
            180                 185                 190         


Cys Glu Ser Leu Val Asn Glu Gly Val Ser Gly Val Ile Thr Gly Phe 
        195                 200                 205             


Thr Ala Asn Glu Ile Met Thr Ala Leu Ala Val Val Ile Thr Lys Leu 
    210                 215                 220                 


Gln Glu Gly Glu Ser Phe Phe Lys Asn Cys Tyr Pro Arg Val Val Thr 
225                 230                 235                 240 


Tyr Glu Gly Ser Lys Ala Ala Gln Thr Ile Val Ser Glu Val Met Glu 
                245                 250                 255     


Asp Cys Asp Ser Glu Trp Arg Gly Leu Gly Val Ile Lys Asn Ser Gly 
            260                 265                 270         


Leu Lys Leu Lys Glu Ala Tyr Lys Glu Phe Asp Ala Arg Asp Lys Phe 
        275                 280                 285             


His Leu Gly Lys Val Glu Gly Arg Ser Asn Pro Ala Cys Arg Cys Gly 
    290                 295                 300                 


Asp Val Leu Gln Gly Lys Cys Lys Pro Ser Asp Cys Lys Val Phe Gly 
305                 310                 315                 320 


Lys Gly Cys Thr Pro Leu His Pro Val Gly Ala Cys Met Val Ser Asn 
                325                 330                 335     


Glu Gly Ala Cys Ser Ala Tyr Tyr Gln Tyr Gly Gly Lys Ile Asn Gly 
            340                 345                 350         


<210>  46
<211>  1059
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_0370, hydrogenase expression/formation protein HypD

<400>  46
atggctgaga acataaaaaa aactgcaaag gagattattg aatcctatga tggaccaaag       60

atccgtatta tggaggtttg cggaacgcat acccatgaga tattcagact tggaatcaga      120

aatataatac cagaaaatat tgaactcata tcaggaccag gatgtccggt ttgtgttaca      180

ccagttggct ttattgatga agctattatg ctggcccttg accataatgc taccatatgt      240

acctttggag atttagtaag agtgcctggt tcagaaatga accttgctgg agccagatca      300

aaaggcgcca aagttcaaat agtatattca cctattgatg cttatgagta tgctagagac      360

caccttgatg agcaggtagt attcttatca gtaggatttg agacaaccac acctgccagc      420

tgtctggctg taaaaaaggc aaaaggtgca gaacttaaaa attttgcatt acttacggca      480

aacaaaacca tgccgggagt atacaaaaca ctaaaaaata gtgcagatgc atttttatat      540

cctggccatg tacatgctat tacaggtacg gagttatgtg agtctttagt taacgaaggc      600

gttagtggag taataacagg ttttactgca aatgaaatta tgacagcact tgctgtagta      660

atcacaaaat tacaagaagg agaaagcttt tttaaaaact gttatccgag agttgtaaca      720

tatgaaggca gtaaagcggc tcagactatt gtatctgaag tcatggagga ctgcgattct      780

gagtggagag gtcttggtgt tatcaaaaat tcaggactta aattgaagga agcttataaa      840

gagtttgatg ccagagacaa atttcattta ggaaaagtag agggaagaag taatccggct      900

tgccgatgtg gtgatgtttt gcagggaaaa tgcaagccat cggattgcaa agtatttggc      960

aaagggtgta cgccactaca tcctgttgga gcatgtatgg tatctaatga aggagcatgt     1020

tcagcatatt atcagtatgg aggaaaaata aatggataa                            1059


<210>  47
<211>  68
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_0371, hydrogenase assembly chaperone hypC/hupF

<400>  47

Met Cys Val Gly Leu Pro Ala Arg Val Val Lys Val Lys Glu Gly Met 
1               5                   10                  15      


Ala Leu Ile Asp Ala Ser Gly Ala Lys Arg Lys Val Ser Ala Glu Leu 
            20                  25                  30          


Ile Asp Glu Leu Glu Pro Gly Asp Tyr Val Met Val His Ala Gly Ile 
        35                  40                  45              


Ala Ile Ser Lys Ile Thr Asn Asp Asp Gln Ser Glu Thr Asp Lys Ile 
    50                  55                  60                  


Met Glu Glu Leu 
65              


<210>  48
<211>  207
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_0371, hydrogenase assembly chaperone hypC/hupF

<400>  48
atgtgtgtag gtttaccagc aagagttgta aaggttaaag aaggaatggc tctaattgat       60

gcttcaggag caaaaagaaa ggtatctgca gaattaatcg atgaattgga accaggcgat      120

tacgttatgg ttcatgcagg aatcgccatt tcaaaaatta caaacgatga tcagagtgaa      180

acagataaaa tcatggagga attataa                                          207


<210>  49
<211>  799
<212>  PRT
<213>  Clostridium autoethanogenum


<220>
<221>  MISC_FEATURE
<223>  CAETHG_0372, (NiFe) hydrogenase maturation protein HypF

<400>  49

Met Lys Asp Tyr Lys Thr Leu Ser Ile Arg Val Tyr Gly Ile Val Gln 
1               5                   10                  15      


Gly Val Gly Phe Arg Pro Thr Val Ser Arg His Ala Asp Lys Asn His 
            20                  25                  30          


Ile Arg Gly Ser Val Cys Asn Lys Gly Pro Tyr Val Glu Ile Trp Ala 
        35                  40                  45              


Gln Gly Ser Glu Ser Glu Leu Glu Gly Phe Leu Tyr Asp Leu Glu His 
    50                  55                  60                  


Asn Pro Pro Lys Arg Ser Ser Ile Leu Lys Ile Asp Val His Lys Glu 
65                  70                  75                  80  


Glu Glu Ser Glu Lys Phe Gln Asp Phe Glu Ile Ile Glu Ser Glu His 
                85                  90                  95      


Val Gln Gly Glu Ile Phe Val Ser Pro Asp Ile Ala Ile Cys Pro Glu 
            100                 105                 110         


Cys Lys Arg Glu Leu Phe Asp Lys Asn Asn Arg Arg Tyr Leu His Pro 
        115                 120                 125             


Phe Ile Asn Cys Thr Cys Cys Gly Pro Arg Leu Thr Ile Leu Asp Ser 
    130                 135                 140                 


Met Pro Tyr Asp Arg Val Arg Thr Ser Met Gly Glu Phe Pro Met Cys 
145                 150                 155                 160 


Pro Glu Cys Glu Tyr Glu Tyr Thr His Ala Glu Thr Arg Arg Tyr Asp 
                165                 170                 175     


Ala Gln Pro Val Cys Cys Asn Glu Cys Gly Pro Glu Val Tyr Leu Ile 
            180                 185                 190         


Gly Arg Gly Glu Arg Gly Arg Thr Ala Ile Thr Tyr Thr Arg Gln Val 
        195                 200                 205             


Ile His Asp Gly Gly Ile Ala Ala Ile Lys Gly Ile Gly Gly Phe His 
    210                 215                 220                 


Leu Cys Cys Asp Ala Thr Asn Gln Glu Ala Val Glu Arg Leu Arg Lys 
225                 230                 235                 240 


Leu Lys Thr Arg Pro Ala Lys Pro Phe Ala Val Met Met Arg Asp Met 
                245                 250                 255     


Glu Thr Val Glu Arg Glu Cys Glu Val Thr Lys Val Gln Lys Glu Val 
            260                 265                 270         


Leu Asp Gly His Gln Lys Pro Ile Ile Leu Leu Lys Arg Lys Gln Ser 
        275                 280                 285             


Lys Met Val Cys Asp Ala Val Thr Pro Asp Asn Pro Lys Ile Gly Val 
    290                 295                 300                 


Met Leu Pro Tyr Ala Pro Val Gln Leu Leu Ile Phe Thr Tyr Asp Asp 
305                 310                 315                 320 


Asp Ile Val Met Pro Asp Cys Leu Val Met Thr Ser Gly Asn Thr Ser 
                325                 330                 335     


Gly Ala Pro Ile Cys Arg Asp Asp Asn Asp Ala Ile Thr Glu Leu Ser 
            340                 345                 350         


Lys Met Cys Asp Val Ile Leu Ser His Asn Arg Met Ile Arg Ile Arg 
        355                 360                 365             


Ala Asp Asp Ser Val Met Asp Phe Phe Glu Glu Lys Pro Tyr Met Ile 
    370                 375                 380                 


Arg Arg Ser Arg Gly Tyr Ala Pro Leu Pro Phe Met Val Ser Asn Gly 
385                 390                 395                 400 


Phe Lys Gly Glu Val Leu Ala Val Gly Gly Glu Leu Lys Asn Thr Phe 
                405                 410                 415     


Cys Ile Gly Lys Asn Asp Leu Phe Tyr Gln Ser Pro Tyr Val Gly Asp 
            420                 425                 430         


Met Glu Asp Leu Arg Thr Val Lys Ala Leu Lys Glu Ser Ile Thr Arg 
        435                 440                 445             


Leu Glu Thr Leu Leu Glu Thr Thr Pro Thr Ile Val Ala Cys Asp Met 
    450                 455                 460                 


His Pro Lys Tyr Asn Thr Thr Cys Ile Ala Gln Glu Ile Gly Ile Pro 
465                 470                 475                 480 


Val Phe Gln Val Gln His His Tyr Ala His Ile Leu Ser Cys Met Ala 
                485                 490                 495     


Glu Asn Asp Tyr Ser Asp Pro Val Ile Gly Val Ser Phe Asp Gly Thr 
            500                 505                 510         


Gly Tyr Gly Thr Asp Ala Thr Ile Trp Gly Gly Glu Leu Leu Glu Val 
        515                 520                 525             


Thr Tyr Asp Gly Phe Glu Arg Leu Gly Ser Ile Lys Pro Phe Ile Gln 
    530                 535                 540                 


Ile Gly Gly Asp Met Ser Ala Lys Glu Gly Trp Arg Ile Ala Val Ser 
545                 550                 555                 560 


Met Ile Tyr Ser Ile Tyr Lys Asp Lys Glu Lys Ala Ala Glu Val Val 
                565                 570                 575     


Arg Gln Leu Lys Leu Cys Asp Glu Lys Asn Cys Asp Val Gln Phe Met 
            580                 585                 590         


Met Ala Asp Asn Lys Ile Asn Ser Ile Thr Ser Thr Ser Ala Gly Arg 
        595                 600                 605             


Leu Phe Asp Ala Val Ser Ala Ile Leu Asn Ile Arg Lys Gln Ser Ser 
    610                 615                 620                 


Phe Glu Gly Glu Ala Ser Thr Thr Leu Glu Phe Ala Ala Glu Ala Tyr 
625                 630                 635                 640 


Glu Glu Lys His Ala Asp His Asn Ser Gly Ser Lys Ile Thr Asp Glu 
                645                 650                 655     


Asp Ser Ile Glu Leu Ser Asn Leu Val Tyr Glu Asn Ser Asn Gly Gln 
            660                 665                 670         


Leu Ile Phe Ala Thr Asp Val Leu Val Lys Lys Ile Ile Glu Glu Thr 
        675                 680                 685             


Leu Thr Gly Lys Asp Ala Ala Met Leu Ala Tyr Phe Phe His Glu Lys 
    690                 695                 700                 


Leu Ser Asp Met Ile Ala Ala Gly Cys Ile Gln Ala Ser Arg Asn Thr 
705                 710                 715                 720 


Gly Ile Lys Thr Ile Ala Leu Ser Gly Gly Val Tyr Gln Asn Gln Leu 
                725                 730                 735     


Leu Leu Lys Met Ser Leu Asp Arg Leu Arg Lys Leu Gly Phe Lys Val 
            740                 745                 750         


Leu Ile His Ser Leu Leu Pro Pro Asn Asp Gly Gly Ile Ala Leu Gly 
        755                 760                 765             


Gln Ala Val Ala Ala Met Glu His Ile Asn Lys Leu Lys Lys Gly Glu 
    770                 775                 780                 


Glu Asn Asn Tyr Val Cys Arg Phe Thr Ser Lys Ser Cys Lys Gly 
785                 790                 795                 


<210>  50
<211>  2400
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_0372, (NiFe) hydrogenase maturation protein HypF

<400>  50
atgaaagatt ataaaacttt atctattaga gtctatggta ttgtgcaggg agttggattt       60

agacctacag taagccggca tgcagataaa aatcatatac ggggaagtgt atgcaataaa      120

ggaccttatg tggaaatatg ggctcaaggt agtgaaagtg aattagaggg ttttttgtac      180

gatttagaac acaatcctcc aaagcgttca tcaatattaa aaattgatgt gcataaagag      240

gaagagtcag aaaaatttca ggactttgaa attattgaaa gtgaacatgt acaaggtgag      300

atttttgtat ctcctgatat tgccatctgt ccagagtgta aaagagaact ttttgataag      360

aataacagac gatatcttca tccctttata aactgtacgt gctgcggacc aagacttact      420

attttagatt ccatgcctta tgatcgtgta cgtaccagca tgggtgaatt tccaatgtgt      480

ccggagtgtg aatatgaata tactcatgca gaaactcgaa gatatgatgc gcagccggta      540

tgctgcaatg aatgcggccc ggaggtttac ttaattggaa gaggcgagcg gggcaggact      600

gcaattactt atacaagaca agtaatccat gatggaggga ttgcagctat aaagggaatt      660

ggaggtttcc atctttgctg tgatgccaca aatcaggaag ctgtagagag gcttagaaaa      720

ttaaagacaa gacctgcaaa gccctttgca gttatgatgc gggacatgga aactgtagaa      780

cgtgaatgtg aagttacaaa agttcaaaaa gaagtactgg atggtcatca gaaaccaatt      840

atacttctga aaagaaagca aagcaaaatg gtgtgtgatg ctgtgacacc tgataatcca      900

aagattggag ttatgcttcc ctatgctcct gttcaacttt tgatatttac atatgatgat      960

gatatagtta tgccggattg cctggttatg acaagtggta atacatcagg tgcaccaata     1020

tgtagagatg acaatgatgc aataacagaa ttatcaaaaa tgtgtgatgt tattttatca     1080

cataacagaa tgattcgtat tcgtgcagat gactcggtta tggatttctt tgaagaaaaa     1140

ccttatatga taaggcgttc cagaggatat gcgccgcttc cgtttatggt ttcaaacgga     1200

tttaaagggg aagtactggc agttggtggt gaactcaaga atactttttg tataggcaaa     1260

aatgatttgt tttatcagtc cccctatgtt ggtgacatgg aagatttgag aacagtaaaa     1320

gcattgaaag agtctattac aagattggaa actctgcttg agacgacgcc aacaattgtg     1380

gcatgtgaca tgcatccaaa gtataatact acgtgcattg cacaggaaat tggaattccc     1440

gtatttcagg ttcaacatca ttatgcacat attttatctt gtatggctga aaatgattat     1500

tctgatccag ttataggcgt ttcatttgat ggaacaggat atggaacaga tgcaaccatc     1560

tggggtggag agctattaga agttacttat gatggatttg aaaggcttgg cagcataaaa     1620

ccttttattc agatcggtgg tgacatgtca gctaaagagg gctggagaat tgcggtatcc     1680

atgatttaca gtatatacaa agataaagaa aaagcagctg aagttgtaag acagcttaaa     1740

ttatgtgatg agaagaactg cgatgttcag tttatgatgg ctgacaacaa aataaacagc     1800

ataacatcta cgagtgcagg gcgtttgttt gatgctgtaa gtgctatctt gaatatcaga     1860

aaacaatcat cttttgaagg ggaagcttct actacccttg aatttgcagc agaggcttat     1920

gaagaaaaac atgcggacca taatagtggc agcaaaatta ctgatgagga tagtattgaa     1980

ctttcaaatc ttgtttatga gaattcaaat ggacagctaa ttttcgcaac agatgtttta     2040

gtaaagaaaa tcatagagga aactttaact gggaaagatg cagcgatgct tgcttacttc     2100

ttccatgaaa aattgtcaga tatgattgca gctggatgta tacaagcttc tagaaacact     2160

ggaatcaaaa ccatagcatt aagcggagga gtatatcaaa accagttatt attaaaaatg     2220

agcctagacc gtttgagaaa acttggattt aaagttttaa ttcatagttt gttgccgcca     2280

aatgatggtg gaatagcact aggtcaggca gtagcagcaa tggaacatat aaataaatta     2340

aaaaaagggg aggagaataa ctatgtgtgt aggtttacca gcaagagttg taaaggttaa     2400


<210>  51
<211>  344
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_1576, sequence of intron targeting region

<400>  51
aagcttataa ttatccttaa ttgtcgttgt agtgcgccca gatagggtgt taagtcaagt       60

agtttaaggt actactctgt aagataacac agaaaacagc caacctaacc gaaaagcgaa      120

agctgatacg ggaacagagc acggttggaa agcgatgagt tacctaaaga caatcgggta      180

cgactgagtc gcaatgttaa tcagatataa ggtataagtt gtgtttactg aacgcaagtt      240

tctaatttcg gttacaatcc gatagaggaa agtgtctgaa acctctagta caaagaaagg      300

taagttagct acaacgactt atctgttatc accacatttg taca                       344


<210>  52
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  forward primer Og119f

<400>  52
tgcttgtctt atcatactta taagc                                             25


<210>  53
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  reverse primer Og120f

<400>  53
tagagaatac acatccagag ttaat                                             25


<210>  54
<211>  344
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_0110, sequence of intron targeting region

<400>  54
aagcttataa ttatccttag aattctgtaa ggtgcgccca gatagggtgt taagtcaagt       60

agtttaaggt actactctgt aagataacac agaaaacagc caacctaacc gaaaagcgaa      120

agctgatacg ggaacagagc acggttggaa agcgatgagt tacctaaaga caatcgggta      180

cgactgagtc gcaatgttaa tcagatataa ggtataagtt gtgtttactg aacgcaagtt      240

tctaatttcg attaattctc gatagaggaa agtgtctgaa acctctagta caaagaaagg      300

taagttaatc ttacagactt atctgttatc accacatttg taca                       344


<210>  55
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  forward primer Og121f

<400>  55
aggcatatta tagtggaaat tttga                                             25


<210>  56
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  reverse primer Og122r

<400>  56
tgtaagcaca atttttctta ttgtc                                             25


<210>  57
<211>  344
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_0860, sequence of intron targeting region

<400>  57
aagcttataa ttatccttac caggcgtgga ggtgcgccca gatagggtgt taagtcaagt       60

agtttaaggt actactctgt aagataacac agaaaacagc caacctaacc gaaaagcgaa      120

agctgatacg ggaacagagc acggttggaa agcgatgagt tacctaaaga caatcgggta      180

cgactgagtc gcaatgttaa tcagatataa ggtataagtt gtgtttactg aacgcaagtt      240

tctaatttcg gttcctggtc gatagaggaa agtgtctgaa acctctagta caaagaaagg      300

taagttatac tccacgactt atctgttatc accacatttg taca                       344


<210>  58
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  forward primer Og117f

<400>  58
agtccttgca taatttctaa aatct                                             25


<210>  59
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  reverse primer Og118r

<400>  59
taagtagtgt tatgttagag gacat                                             25


<210>  60
<211>  344
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_3569, sequence of intron targeting region

<400>  60
aagcttataa ttatccttaa ttgacgttct agtgcgccca gatagggtgt taagtcaagt       60

agtttaaggt actactctgt aagataacac agaaaacagc caacctaacc gaaaagcgaa      120

agctgatacg ggaacagagc acggttggaa agcgatgagt tacctaaaga caatcgggta      180

cgactgagtc gcaatgttaa tcagatataa ggtataagtt gtgtttactg aacgcaagtt      240

tctaatttcg gtttcaatcc gatagaggaa agtgtctgaa acctctagta caaagaaagg      300

taagttatat agaacgactt atctgttatc accacatttg taca                       344


<210>  61
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  forward primer Og125f

<400>  61
taatccattc ataagcagtt ctaag                                             25


<210>  62
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  reverse primer Og126r

<400>  62
ttactttagg tgaaaaattc ggtat                                             25


<210>  63
<211>  325
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_3841, sequence of intron targeting region

<400>  63
aagcttataa ttatccttag atgtccagat agtgcgccca gatagggtgt taagtcaagt       60

agtttaaggt actactctgt aagataacac agaaaacagc caacctaacc gaaaagcgaa      120

agctgatacg ggaacagagc acggttggaa agcgatgagt tacctaaaga caatcgggta      180

cgactgagtc gcaatgttaa tcagatataa ggtataagtt gtgtttactg aacgcaagtt      240

tctaatttcg attacatctc gatagaggaa agtgtctgaa acctctagta caaagaaagg      300

taagttaaat atctggactt atctg                                            325


<210>  64
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  forward primer Og123f

<400>  64
taaatgtgat ttgtgtattg atcag                                             25


<210>  65
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  reverse primer Og124r

<400>  65
ctctttaaca gatggaagca tatta                                             25


<210>  66
<211>  309
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_2797, sequence of intron targeting region

<400>  66
ttatccttac acaacgtctg cgtgcgccca gatagggtgt taagtcaagt agtttaaggt       60

actactctgt aagataacac agaaaacagc caacctaacc gaaaagcgaa agctgatacg      120

ggaacagagc acggttggaa agcgatgagt tacctaaaga caatcgggta cgactgagtc      180

gcaatgttaa tcagatataa ggtataagtt gtgtttactg aacgcaagtt tctaatttcg      240

gttttgtgtc gatagaggaa agtgtctgaa acctctagta caaagaaagg taagttaatg      300

cagacgact                                                              309


<210>  67
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  forward primer FS001for

<400>  67
tagccgatcc tgacaagtgc                                                   20


<210>  68
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  reverse primer FS001rev

<400>  68
aagcaggttc tcctccctct                                                   20


<210>  69
<211>  309
<212>  DNA
<213>  Clostridium autoethanogenum


<220>
<221>  misc_feature
<223>  CAETHG_2798, sequence of intron targeting region

<400>  69
ttatccttag gagtcttgcc agtgcgccca gatagggtgt taagtcaagt agtttaaggt       60

actactctgt aagataacac agaaaacagc caacctaacc gaaaagcgaa agctgatacg      120

ggaacagagc acggttggaa agcgatgagt tacctaaaga caatcgggta cgactgagtc      180

gcaatgttaa tcagatataa ggtataagtt gtgtttactg aacgcaagtt tctaatttcg      240

attactcctc gatagaggaa agtgtctgaa acctctagta caaagaaagg taagttaatt      300

ggcaagact                                                              309


<210>  70
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  forward primer FS003for

<400>  70
tggaacttta agtgaaggaa aaatgg                                            26


<210>  71
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  reverse primer FS003rev

<400>  71
tttcaaattc cttgcatgta catgg                                             25


