                         SEQUENCE LISTING

<110>  Technische Universitt Wien
 
<120>  Verfahren zur Herstellung von Sekundrmetaboliten

<130>  48479

<150>  A 51115/2015
<151>  2015-12-30

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  504
<212>  PRT
<213>  Trichoderma reesei

<400>  1

Met Ala Gln Ala Leu Asp Ile Ser Ser Ser Ser Gly Pro Gly Pro Leu 
1               5                   10                  15      


Val Ala Leu Pro Glu Arg Thr Leu Gly Pro Pro Ala Ser Ala Ile Thr 
            20                  25                  30          


Pro Pro Ser Thr Val Gln Arg Ser Ala Trp Thr Ser Ser Pro Arg Ala 
        35                  40                  45              


Pro Ala Met Asp Met Phe Asn Phe Thr Thr Ala Ala Ser Ala Ser Ala 
    50                  55                  60                  


Val Ala Ser Tyr Ala Pro Ala Gly Gly Ser Asn Ser Asn Ser Asn Ser 
65                  70                  75                  80  


Asn Ser Gly Gly Asn Ser Gly Gly Leu Gly Asp Met Asn Phe Ser Gln 
                85                  90                  95      


Met Pro Gln Tyr Ser Gly Arg Asn Pro Leu Gln Ser Met Leu Ser Gln 
            100                 105                 110         


Thr Lys Glu Gly Pro Asp Ala Lys Arg Ile Lys Val Glu Ser Pro His 
        115                 120                 125             


Ser Ala Leu Asp Ser Ile Asp Ser Trp Leu Gln Phe Asp Glu Asp Ala 
    130                 135                 140                 


Asp Lys Phe Gly Ser Phe Glu Ile Asp Phe Ser Lys Gln Tyr Asn Thr 
145                 150                 155                 160 


Ala Asn Gln Pro Arg Ala Ser Thr Asn Met Thr Pro Gly Leu Gly Ser 
                165                 170                 175     


Gly Leu Tyr Ala Asn Pro Thr Ala Pro Phe Arg Glu Glu Asp Phe Leu 
            180                 185                 190         


Asp Asp Ser Ala Phe Asp His Ser Leu Ser Glu Asp Asp Glu Met Phe 
        195                 200                 205             


Asp Asn Ile Asn Ile Asn Asp Gln Leu Ser Lys Leu Gly Thr Leu Pro 
    210                 215                 220                 


Ser Thr Glu Ala Gln Ser Ser Arg Ser Leu Phe Ser Thr Pro Ser Met 
225                 230                 235                 240 


Gly Trp Glu Lys Pro Gln Gln Gly Val Gln Pro Ser Ala Val Met Gly 
                245                 250                 255     


Asp Glu Ile Pro Arg Arg Met Thr Ser Asp Ser Trp Asp Pro Ile Pro 
            260                 265                 270         


Gln Gly Thr Asn Tyr Thr Leu Thr Pro Asp Glu Arg Arg Arg Leu Leu 
        275                 280                 285             


Glu Ile Ala Met Gly Pro Gly Arg Leu Ala Ser Tyr Val Asn Pro Ser 
    290                 295                 300                 


Arg Phe Asn Met Gly Phe Gly Ser Thr Met Gln Pro Ser Ile Ser Gln 
305                 310                 315                 320 


Glu Phe Gly Gly Phe Gly Ala Pro Lys Gln Gly Leu Ser His Met Gly 
                325                 330                 335     


Met Tyr Gln Arg Asn Asn Ser Ala Asp Thr Ser Ala Ser Pro Leu Ser 
            340                 345                 350         


Gln Ser Arg Glu Leu Lys His Lys Gln Pro Lys Val Glu Thr Pro Pro 
        355                 360                 365             


Lys Thr Val Pro Thr Lys Arg Pro Ser Thr Leu Ser Glu Thr Ser Ser 
    370                 375                 380                 


Ile Gly Lys Glu Lys Val Lys Ser Ala Asp Arg Met Ala His Asn Asp 
385                 390                 395                 400 


Val Glu Arg Lys Tyr Arg Thr Asn Leu Lys Asp Lys Ile Thr Glu Leu 
                405                 410                 415     


Arg Asn Ala Val Pro Ala Leu Gln Gln Val Ala Glu Gly Glu Pro Asp 
            420                 425                 430         


Ala Ala Gln Gly Ile Ala Lys Val Ser Lys Gly Thr Val Leu Thr Lys 
        435                 440                 445             


Ala Thr Glu Tyr Ile His Gln Leu Glu Gln Arg Asn Lys Asp Ile Met 
    450                 455                 460                 


Thr Glu His Lys Gln Leu Met Arg Arg Leu Gln Ala Phe Glu Ala Leu 
465                 470                 475                 480 


Leu Asn Asn Ser Met Arg Val Asp Ile Met Pro Ser His Ser Met Thr 
                485                 490                 495     


Leu Phe Asp Pro Arg Gly Phe Cys 
            500                 


<210>  2
<211>  3655
<212>  DNA
<213>  Trichoderma reesei

<400>  2
cagatggtga agacgggcag aaaggggagc gaaagctgaa aaatacttaa gcagcagctg       60

ttccccgtac gttcgtggag atggcaagag cagccggccg cggggagtct ggagcaggag      120

ccgcagctgc gtagctgccg gttgctgctg cccatctgcc caggttcgtt tgcgccggat      180

ccgtccaaat atctcctgat aaatgcggcc acttttccct catgctcgtc cttttttatt      240

ccccgaagcg cagcaaacga gcagcaagag caaagagcag agcagaaagt cgcctcatct      300

cgggcgccgc cttgcctaac ggggtagcaa taagcccatc gacaccgacg gcgggcggcg      360

ggctggccaa tcagcggcgc gcggtgctgt tcctttggcc cttggagcct tggggaatca      420

tacgatgtct gagcagccaa ttcttgcatt cttcccacat gcatggcatc ccatggcgca      480

gggtcccccc cccttctccc ctctccgctc tcttctcctc tcttcttggc tgaggtgctc      540

tgctccctct gctcggccag ctaagacaac cagcgctgtg gcccggcaag acagccactc      600

ttgtcattcg ccaggggggt tggaggaggg cgccacggct ccgcggaaag tcaggccatt      660

ttcatcaaca gccgcagaca ctggcccaga gaactatcct tatcctggac gcgcgcacgt      720

cgaaaatttt catttttgct cgcccaggcg cgcctgcaat tcgccatgcg ggctgctcga      780

tacccacgca gacggcaaat gacaggccat tctggctcac agacgccgcc gtcccggctc      840

tcgccgaggt ggaaaagggt catctgggtt cgttgcgctg agctctagct aggccagaag      900

acacagccga gacatgccta ttcgacgtgc ctacggggcg gaggcagagg gcagaggaca      960

ggagggcagg aggacatccc aacaaaggct ccccatgccg gaaacgcaaa gagaagcatc     1020

ggagagctgc gaagcacccg ccgaaacagc cactacctta cccagcaaga gcagcagctc     1080

tcttgaatgg cctgactggc gacaccgcgg gtccctagcg ccggggcctt gcctggatgg     1140

caccggtccc tggagcggga tcctcgtcgc tggtgctcga cgggctgtgg tgctgctgtg     1200

cgtcttgcgc tgccggctgg gctgggctgg gctgggctgg atctgagctg cttgcagcgc     1260

cgttccctca ggcttcggaa tcgcagccgt cgcggcgttg cccgaacagc cacccggagc     1320

ttggcctgcc tctgcgaggt gctaatgctg aaactggtcg accctgtata ttacccgtga     1380

tgctctggca ccgcagctcc agacccagac ccgggtctag gttcccaata tcgatcctgt     1440

agcattgata tcgaacggta cttgctgggt actacctccc actcttgctg ctcggtaccc     1500

tgccctgtct cacctgtgca tgcacagaag caccatcact atccattgac ttccaatttt     1560

gcatctgcat ccgtcggcct atcttgccgg catctacttc tacttctact tctacgtctc     1620

cgcctgcact cgcattgacc tctttttgca tctacatctg cggcagagct gtgcgctatc     1680

taccttacct cgtcttgaac ccccctgatc cgagcatcgt atccctccat aaccacctcg     1740

gctcgctacc tcacctcctt cgtgataggt attcaatcgt ggtgtctctt gctcatctgc     1800

atctggaatt cttccctttc tcctcttgac atcttctacc tattatcgcg ctcttcctcc     1860

tccgtctgca accccccaga cgccgccgaa cccgatctgt ggctgccatt agcctttcgg     1920

cgcgcattcc tctacgccac ccggtttggg tcccccttca cccccagcac atattccgac     1980

aaccctgtgt ctgcagccgc atggcacaag ccctcgacat ttcctcgtcg tcaggacccg     2040

gacccctcgt cgccctgccc gagcgaactc tcggcccccc agcctcggcc atcacgcctc     2100

cctcgaccgt ccagcgctcg gcctggacgt cgtcgcctcg cgcgcccgcc atggacatgt     2160

tcaacttcac aaccgctgcc tcggcatccg cagtcgcctc gtatgccccc gccggcggca     2220

gcaatagcaa tagcaatagc aatagcggcg gcaacagcgg cggcctgggc gacatgaact     2280

tctctcagat gccgcagtac tctggccgca atcccctgca gtcgatgctc tcccagacca     2340

aggagggccc cgatgccaag cggataaagg tcgagtcgcc gcactcggcg ctggattcga     2400

tcgattcatg gcttcagttt gacgaggatg ccgacaagtt tggcagcttc gagattgact     2460

tttccaaaca gtacaacacg gccaaccagc caaggtgagc tgcgctcctg aaccgccaat     2520

ctggtgtttc ccgctctcta acacattgcg acattaagag cttccacaaa catgacgccg     2580

ggactgggca gcggtctcta cgccaatccc accgccccct tcagagaaga agactttctc     2640

gacgacagcg cctttgacca ctctctgtcc gaagacgacg agatgttcga caacatcaac     2700

atcaatgacc agctgtccaa gcttggcacg ctgccctcga ccgaagctca gtcatccagg     2760

agtctcttct ccacgccgtc gatgggctgg gagaagcctc agcaaggcgt ccagcccagc     2820

gccgtcatgg gcgacgagat cccgaggcgc atgactagcg attcctggga cccgattccg     2880

cagggcacca attacaccct cacgccggac gagcgccgcc gactgctcga aatcgccatg     2940

ggccccggca gattggcgtc ctacgtcaac cccagccggt tcaacatggg cttcggatct     3000

acgatgcagc ccagcataag ccaggagttt ggcttcggct tcggcgctcc gaaacagggg     3060

ctgagccata tgggcatgta ccagaggaac aactcggccg acacgagcgc atcccccctc     3120

tcgcagagtc gggaattgaa acacaagcag cccaaggtcg agacgccgcc gaagacggtg     3180

cccacaaagc ggcccagcac gctttcggag acgagcagca tcggcaaaga aaaggtcaag     3240

tccgccgacc gcatggcgca caacgacgtt gagcgcaagt atcgaaccaa tctcaaggac     3300

aagattaccg agctgcgaaa tgcggtgccg gcgctgcagc aggtggctga aggggagccg     3360

gacgctgcgc agggcattgc caaagtgagc aaggtgagtt ggaaaacagc atgccccatc     3420

ttgtcagtga ctcgtgaaga cggcatcact gactctgatc ctatagggca ccgtattgac     3480

aaaagcgact gaatacattc atcagctcga gcaacgcaac aaggatatca tgacggagca     3540

taagcagctt atgcgacgac tgcaagcctt cgaagcactt ctcaacaact cgatgagagt     3600

cgacatcatg cccagccata gcatgacgct cttcgacccg agaggattct gttga          3655


<210>  3
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is V or I

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa is D or V

<400>  3

Ala His Asn Asp Xaa Glu Arg Lys Tyr Arg Thr Asn Leu Lys Xaa Lys 
1               5                   10                  15      


Ile 
    


<210>  4
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is V or I

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is T or A

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is  V or I

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa can be any amino acid residue

<400>  4

Lys Xaa Ser Lys Gly Xaa Xaa Leu Xaa Lys Ala Thr Glu Tyr Ile 
1               5                   10                  15  


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is R or K

<400>  5

Arg Xaa Leu Gln Ala Phe 
1               5       


<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide

<400>  6

Leu Phe Asp Pro Arg Gly Phe Cys 
1               5               


<210>  7
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is F or Y

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is  D or E

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is F or Y

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is R or K

<400>  7

Gly Ser Xaa Xaa Ile Asp Xaa Ser Xaa 
1               5                   


<210>  8
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is S or T

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is V, I or L

<400>  8

Pro Gly Leu Gly Xaa Gly Xaa Tyr 
1               5               


<210>  9
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide


<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa is R or K

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa is I or L

<400>  9

Arg Glu Gly Leu Tyr Ser Thr Pro Leu Ser Trp Glu Xaa Pro Gln Pro 
1               5                   10                  15      


Gly Xaa Arg Met Asp 
            20      


<210>  10
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Xaa can be any naturally occurring amino acid

<400>  10

Gly Arg Ala Pro Gln Leu Ser Gln Gln Gln Xaa Gln Gln Gln Gln Gln 
1               5                   10                  15      


Gln 
    


<210>  11
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is R or K

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa is S or T

<400>  11

Pro Pro Pro Glu Val Pro Pro Xaa Glu Gly Leu Tyr Ser Thr Pro Leu 
1               5                   10                  15      


Xaa Trp Glu 
            


<210>  12
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is A or P

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa is L or M

<400>  12

Pro Ser Thr Glu Xaa Gln Ser Ser Arg Ser Leu Tyr Ser Thr Pro Ser 
1               5                   10                  15      


Xaa Gly Trp Glu 
            20  


<210>  13
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide

<400>  13

Thr Lys Ala Lys Pro Gly Glu Lys Met Ala Gln Leu Glu Ser Ile Ser 
1               5                   10                  15      


Thr Ser Gln Gln Pro Leu Asp 
            20              


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide


<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Xaa can be any naturally occurring amino acid

<400>  14

Ala Leu Glu Asn Ser Ala Gln Gly Xaa Asp Lys Phe Gln Pro Gly 
1               5                   10                  15  


<210>  15
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is V or I

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa is D or V

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa is a peptide having 20 to 40 amino acid residues

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa is V or I

<220>
<221>  MISC_FEATURE
<222>  (24)..(24)
<223>  Xaa is T or A

<220>
<221>  MISC_FEATURE
<222>  (25)..(25)
<223>  Xaa is V or I

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (34)..(34)
<223>  Xaa is a peptide having 15 o 20 amino acid residues

<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (41)..(41)
<223>  Xaa is a peptide having 15 to 25 amino acid residues

<400>  15

Ala His Asn Asp Xaa Glu Arg Lys Tyr Arg Thr Asn Leu Lys Xaa Lys 
1               5                   10                  15      


Ile Xaa Lys Xaa Ser Lys Gly Xaa Xaa Leu Xaa Lys Ala Thr Glu Tyr 
            20                  25                  30          


Ile Xaa Arg Xaa Leu Gln Ala Phe Xaa Leu Phe Asp Pro Arg Gly Phe 
        35                  40                  45              


Cys 
    


<210>  16
<211>  59
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial Peptide


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is F or Y

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is D or E

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is F or Y

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is R or K

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa is a peptide having 200 to 300 amino acid residues

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa is V or I

<220>
<221>  MISC_FEATURE
<222>  (25)..(25)
<223>  Xaa is D or V

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa is a peptide having 20 to 40 amino acid residues

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa is V or I

<220>
<221>  MISC_FEATURE
<222>  (34)..(34)
<223>  Xaa is T or A

<220>
<221>  MISC_FEATURE
<222>  (35)..(35)
<223>  Xaa is V or I

<220>
<221>  misc_feature
<222>  (37)..(37)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (44)..(44)
<223>  Xaa is a peptide having 15 o 20 amino acid residues

<220>
<221>  misc_feature
<222>  (46)..(46)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (51)..(51)
<223>  Xaa is a peptide having 15 to 25 amino acid residues

<400>  16

Gly Ser Xaa Xaa Ile Asp Xaa Ser Xaa Xaa Ala His Asn Asp Xaa Glu 
1               5                   10                  15      


Arg Lys Tyr Arg Thr Asn Leu Lys Xaa Lys Ile Xaa Lys Xaa Ser Lys 
            20                  25                  30          


Gly Xaa Xaa Leu Xaa Lys Ala Thr Glu Tyr Ile Xaa Arg Xaa Leu Gln 
        35                  40                  45              


Ala Phe Xaa Leu Phe Asp Pro Arg Gly Phe Cys 
    50                  55                  


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  17
tgagagcggt ggtatccacg                                                   20


<210>  18
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  18
ggtaccacca gacatgacaa tgttg                                             25


<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  19
tggatcgtca actggttcta cga                                               23


<210>  20
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  20
gcatgtgtag caacgtggtc ttt                                               23


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  21
agctactcaa caacgtgacg c                                                 21


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  22
atcccactgc tgctcaggta c                                                 21


<210>  23
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  23
ggcctttgtt cttcatgact cc                                                22


<210>  24
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  24
gtgagccaag gcatcttcg                                                    19


<210>  25
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  25
cggtatgata tcagaggttg gc                                                22


<210>  26
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  26
ctgggccatg agcttgc                                                      17


<210>  27
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  27
tagccaggcg aattatgcg                                                    19


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  28
ggtcctgggt atcgcctatc                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  29
agatttctga cgcggacctc                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  30
agggcaatag acttgacggg                                                   20


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  31
taccaactct tcgcaaacgt g                                                 21


<210>  32
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  32
ccaggagtga tccagaagaa gtc                                               23


