                         SEQUENCE LISTING

<110>  DuPont Nutrition Biosciences ApS
       SOE, Jorn Borch
       MIKKELSEN, Rene
       JORGENSEN, Tina
 
<120>  IMPROVED ENZYMATIC MODIFICATION OF WHEAT PHOSPHOLIPIDS IN BAKERY 
       APPLICATIONS

<130>  NB40992-WO-PCT

<150>  62/319399
<151>  2016-04-07

<150>  GB 1522681.4
<151>  2015-12-22

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  279
<212>  PRT
<213>  Fusarium heterosporum


<220>
<221>  MISC_FEATURE
<223>  Phospholipase sn1;enzyme in POWERBAKE 4080 and POWERBAKE 4090 
       that acts on a polar lipid at the sn1 position (same as SEQ ID 
       NO: 6 from US Patent 8,012,732; hereby incorporated by 
       reference).  This enzyme is known to have both gala

<400>  1

Glu Ala Glu Ala Ala Val Gly Val Thr Ser Thr Asp Phe Thr Asn Phe 
1               5                   10                  15      


Lys Phe Tyr Ile Gln His Gly Ala Ala Ala Tyr Cys Asn Ser Gly Thr 
            20                  25                  30          


Ala Ala Gly Ala Lys Ile Thr Cys Ser Asn Asn Gly Cys Pro Thr Ile 
        35                  40                  45              


Glu Ser Asn Gly Val Thr Val Val Ala Ser Phe Thr Gly Ser Lys Thr 
    50                  55                  60                  


Gly Ile Gly Gly Tyr Val Ser Thr Asp Ser Ser Arg Lys Glu Ile Val 
65                  70                  75                  80  


Val Ala Ile Arg Gly Ser Ser Asn Ile Arg Asn Trp Leu Thr Asn Leu 
                85                  90                  95      


Asp Phe Asp Gln Ser Asp Cys Ser Leu Val Ser Gly Cys Gly Val His 
            100                 105                 110         


Ser Gly Phe Gln Asn Ala Trp Ala Glu Ile Ser Ala Gln Ala Ser Ala 
        115                 120                 125             


Ala Val Ala Lys Ala Arg Lys Ala Asn Pro Ser Phe Lys Val Val Ala 
    130                 135                 140                 


Thr Gly His Ser Leu Gly Gly Ala Val Ala Thr Leu Ser Ala Ala Asn 
145                 150                 155                 160 


Leu Arg Ala Ala Gly Thr Pro Val Asp Ile Tyr Thr Tyr Gly Ala Pro 
                165                 170                 175     


Arg Val Gly Asn Ala Ala Leu Ser Ala Phe Ile Ser Asn Gln Ala Gly 
            180                 185                 190         


Gly Glu Phe Arg Val Thr His Asp Lys Asp Pro Val Pro Arg Leu Pro 
        195                 200                 205             


Pro Leu Ile Phe Gly Tyr Arg His Thr Thr Pro Glu Tyr Trp Leu Ser 
    210                 215                 220                 


Gly Gly Gly Gly Asp Lys Val Asp Tyr Ala Ile Ser Asp Val Lys Val 
225                 230                 235                 240 


Cys Glu Gly Ala Ala Asn Leu Met Cys Asn Gly Gly Thr Leu Gly Leu 
                245                 250                 255     


Asp Ile Asp Ala His Leu His Tyr Phe Gln Ala Thr Asp Ala Cys Asn 
            260                 265                 270         


Ala Gly Gly Phe Ser Trp Arg 
        275                 


<210>  2
<211>  318
<212>  PRT
<213>  Aeromonas salmonicida


<220>
<221>  MISC_FEATURE
<223>  mature lipid acyltransferase (GCAT) derived from Aeromonas 
       salmonicida (See US patent 9,175,271)

<400>  2

Ala Asp Thr Arg Pro Ala Phe Ser Arg Ile Val Met Phe Gly Asp Ser 
1               5                   10                  15      


Leu Ser Asp Thr Gly Lys Met Tyr Ser Lys Met Arg Gly Tyr Leu Pro 
            20                  25                  30          


Ser Ser Pro Pro Tyr Tyr Glu Gly Arg Phe Ser Asn Gly Pro Val Trp 
        35                  40                  45              


Leu Glu Gln Leu Thr Lys Gln Phe Pro Gly Leu Thr Ile Ala Asn Glu 
    50                  55                  60                  


Ala Glu Gly Gly Ala Thr Ala Val Ala Tyr Asn Lys Ile Ser Trp Asp 
65                  70                  75                  80  


Pro Lys Tyr Gln Val Ile Asn Asn Leu Asp Tyr Glu Val Thr Gln Phe 
                85                  90                  95      


Leu Gln Lys Asp Ser Phe Lys Pro Asp Asp Leu Val Ile Leu Trp Val 
            100                 105                 110         


Gly Ala Asn Asp Tyr Leu Ala Tyr Gly Trp Asn Thr Glu Gln Asp Ala 
        115                 120                 125             


Lys Arg Val Arg Asp Ala Ile Ser Asp Ala Ala Asn Arg Met Val Leu 
    130                 135                 140                 


Asn Gly Ala Lys Gln Ile Leu Leu Phe Asn Leu Pro Asp Leu Gly Gln 
145                 150                 155                 160 


Asn Pro Ser Ala Arg Ser Gln Lys Val Val Glu Ala Val Ser His Val 
                165                 170                 175     


Ser Ala Tyr His Asn Lys Leu Leu Leu Asn Leu Ala Arg Gln Leu Ala 
            180                 185                 190         


Pro Thr Gly Met Val Lys Leu Phe Glu Ile Asp Lys Gln Phe Ala Glu 
        195                 200                 205             


Met Leu Arg Asp Pro Gln Asn Phe Gly Leu Ser Asp Val Glu Asn Pro 
    210                 215                 220                 


Cys Tyr Asp Gly Gly Tyr Val Trp Lys Pro Phe Ala Thr Arg Ser Val 
225                 230                 235                 240 


Ser Thr Asp Arg Gln Leu Ser Ala Phe Ser Pro Gln Glu Arg Leu Ala 
                245                 250                 255     


Ile Ala Gly Asn Pro Leu Leu Ala Gln Ala Val Ala Ser Pro Met Ala 
            260                 265                 270         


Arg Arg Ser Ala Ser Pro Leu Asn Cys Glu Gly Lys Met Phe Trp Asp 
        275                 280                 285             


Gln Val His Pro Thr Thr Val Val His Ala Ala Leu Ser Glu Arg Ala 
    290                 295                 300                 


Ala Thr Phe Ile Glu Thr Gln Tyr Glu Phe Leu Ala His Gly 
305                 310                 315             


<210>  3
<211>  124
<212>  PRT
<213>  Aspergillus niger


<220>
<221>  MISC_FEATURE
<223>  phospholipase A2 enzyme which acts on N-acyl phosphatidyl 
       ethanolamine at the sn2 position found in MAXAPAL A2.

<400>  3

Ala Leu Trp Gln Phe Arg Ser Met Ile Lys Cys Ala Ile Pro Gly Ser 
1               5                   10                  15      


His Pro Leu Met Asp Phe Asn Asn Tyr Gly Cys Tyr Cys Gly Leu Gly 
            20                  25                  30          


Gly Ser Gly Thr Pro Val Asp Glu Leu Asp Arg Cys Cys Glu Thr His 
        35                  40                  45              


Asp Asn Cys Tyr Arg Asp Ala Lys Asn Leu Asp Ser Cys Lys Phe Leu 
    50                  55                  60                  


Val Asp Asn Pro Tyr Thr Glu Ser Tyr Ser Tyr Ser Cys Ser Asn Thr 
65                  70                  75                  80  


Glu Ile Thr Cys Asn Ser Lys Asn Asn Ala Cys Glu Ala Phe Ile Cys 
                85                  90                  95      


Asn Cys Asp Arg Asn Ala Ala Ile Cys Phe Ser Lys Ala Pro Tyr Asn 
            100                 105                 110         


Lys Glu His Lys Asn Leu Asp Thr Lys Lys Tyr Cys 
        115                 120                 


<210>  4
<211>  167
<212>  PRT
<213>  Myceliophthora thermophila


<220>
<221>  MISC_FEATURE
<223>  phospholipase A2 enzyme (CRC08335) which acts on NAPE (N-acyl 
       phosphatidyl ethanolamine) at the sn2 position

<400>  4

Leu Pro Ser Ile Gly Lys Ala Asp Ala Ala Leu Val Pro Arg Gln Ser 
1               5                   10                  15      


Ala Ile Gln Ile Thr Asp Gln Tyr Leu Phe Asp Leu Thr Leu Pro Ala 
            20                  25                  30          


Phe Thr Ala Lys Arg Asn Ala Arg Asp Pro Pro Ser Leu Ile Trp Asp 
        35                  40                  45              


Ser Asp Gly Cys Ser Ser Ser Pro Asp Asn Pro Phe Gly Phe Pro Phe 
    50                  55                  60                  


Val Pro Ala Cys His Arg His Asp Phe Gly Tyr Arg Asn Tyr Lys Ala 
65                  70                  75                  80  


Gln Asn Arg Phe Thr Asp Ala Gly Lys Leu Ser Ile Asp Asn Asn Phe 
                85                  90                  95      


Lys Ser Asp Leu Tyr Tyr Gln Cys Glu Ser Val Ser Ala Lys Thr Ala 
            100                 105                 110         


Cys Arg Ala Leu Ala Asp Val Tyr Tyr Ala Ala Val Arg Ala Phe Gly 
        115                 120                 125             


Gly Ser Thr Gln Asp Lys Arg Asp Asp Asp Leu Val Lys Ile Tyr Glu 
    130                 135                 140                 


Glu Lys Val Ala Ile Tyr Asn Lys Ala Val Glu Glu Ala Gln Ala Lys 
145                 150                 155                 160 


Gly Glu Leu Trp Thr Leu Asp 
                165         


<210>  5
<211>  549
<212>  DNA
<213>  Myceliophthora thermophila


<220>
<221>  misc_feature
<223>  synthetic gene encoding a fungal phospholipase A2 enzyme 
       (CRC08335) which acts on NAPE (N-acyl phosphatidyl ethanolamine) 
       at the sn2 position

<400>  5
atgaagttcc tgagcaccgc actttgcctt gcatcttctg tcctggcact tccatctatc       60

ggtaaagcag acgcagcact tgttccacgt caatctgcaa tccagatcac cgaccagtac      120

cttttcgacc ttacccttcc agcattcacc gcaaaacgta acgcacggga tccaccatct      180

cttatctggg attctgacgg ctgtagctct tctccagata acccattcgg cttcccattc      240

gttcctgctt gtcatcggca tgatttcggt taccggaact acaaggcaca gaaccgtttc      300

accgacgcag gcaagctttc tattgacaac aacttcaaga gcgacctcta ctaccagtgc      360

gagtctgttt ctgcaaagac agcttgtcgg gcacttgcag acgtctatta cgcagcagtt      420

cgggcattcg gaggttctac acaagataag cgggacgacg atctagttaa gatctacgaa      480

gagaaggtcg ccatctacaa caaggcagtt gaagaggcac aagcaaaggg cgagctttgg      540

acacttgat                                                              549


<210>  6
<211>  16
<212>  PRT
<213>  Myceliophthora thermophila


<220>
<221>  MISC_FEATURE
<223>  N-terminal signal peptide sequence per prediction

<400>  6

Met Lys Phe Leu Ser Thr Ala Leu Cys Leu Ala Ser Ser Val Leu Ala 
1               5                   10                  15      


