               SEQUENCE LISTING

<110> DSM IP ASSETS B.V.

<120> HOST CELLS FOR DICARBOXYLIC ACID PRODUCTION

<160> 70

<170> BiSSAP 1.2

<210> 1
<211> 548
<212> PRT
<213> Escherichia coli


<400> 1
Met Ser Asn Lys Pro Phe Ile Tyr Gln Ala Pro Phe Pro Met Gly Lys 
1               5                   10                  15      
Asp Asn Thr Glu Tyr Tyr Leu Leu Thr Ser Asp Tyr Val Ser Val Ala 
            20                  25                  30          
Asp Phe Asp Gly Glu Thr Ile Leu Lys Val Glu Pro Glu Ala Leu Thr 
        35                  40                  45              
Leu Leu Ala Gln Gln Ala Phe His Asp Ala Ser Phe Met Leu Arg Pro 
    50                  55                  60                  
Ala His Gln Lys Gln Val Ala Ala Ile Leu His Asp Pro Glu Ala Ser 
65                  70                  75                  80  
Glu Asn Asp Lys Tyr Val Ala Leu Gln Phe Leu Arg Asn Ser Glu Ile 
                85                  90                  95      
Ala Ala Lys Gly Val Leu Pro Thr Cys Gln Asp Thr Gly Thr Ala Ile 
            100                 105                 110         
Ile Val Gly Lys Lys Gly Gln Arg Val Trp Thr Gly Gly Gly Asp Glu 
        115                 120                 125             
Glu Thr Leu Ser Lys Gly Val Tyr Asn Thr Tyr Ile Glu Asp Asn Leu 
    130                 135                 140                 
Arg Tyr Ser Gln Asn Ala Ala Leu Asp Met Tyr Lys Glu Val Asn Thr 
145                 150                 155                 160 
Gly Thr Asn Leu Pro Ala Gln Ile Asp Leu Tyr Ala Val Asp Gly Asp 
                165                 170                 175     
Glu Tyr Lys Phe Leu Cys Val Ala Lys Gly Gly Gly Ser Ala Asn Lys 
            180                 185                 190         
Thr Tyr Leu Tyr Gln Glu Thr Lys Ala Leu Leu Thr Pro Gly Lys Leu 
        195                 200                 205             
Lys Asn Phe Leu Val Glu Lys Met Arg Thr Leu Gly Thr Ala Ala Cys 
    210                 215                 220                 
Pro Pro Tyr His Ile Ala Phe Val Ile Gly Gly Thr Ser Ala Glu Thr 
225                 230                 235                 240 
Asn Leu Lys Thr Val Lys Leu Ala Ser Ala His Tyr Tyr Asp Glu Leu 
                245                 250                 255     
Pro Thr Glu Gly Asn Glu His Gly Gln Ala Phe Arg Asp Val Gln Leu 
            260                 265                 270         
Glu Gln Glu Leu Leu Glu Glu Ala Gln Lys Leu Gly Leu Gly Ala Gln 
        275                 280                 285             
Phe Gly Gly Lys Tyr Phe Ala His Asp Ile Arg Val Ile Arg Leu Pro 
    290                 295                 300                 
Arg His Gly Ala Ser Cys Pro Val Gly Met Gly Val Ser Cys Ser Ala 
305                 310                 315                 320 
Asp Arg Asn Ile Lys Ala Lys Ile Asn Arg Glu Gly Ile Trp Ile Glu 
                325                 330                 335     
Lys Leu Glu His Asn Pro Gly Gln Tyr Ile Pro Gln Glu Leu Arg Gln 
            340                 345                 350         
Ala Gly Glu Gly Glu Ala Val Lys Val Asp Leu Asn Arg Pro Met Lys 
        355                 360                 365             
Glu Ile Leu Ala Gln Leu Ser Gln Tyr Pro Val Ser Thr Arg Leu Ser 
    370                 375                 380                 
Leu Thr Gly Thr Ile Ile Val Gly Arg Asp Ile Ala His Ala Lys Leu 
385                 390                 395                 400 
Lys Glu Leu Ile Asp Ala Gly Lys Glu Leu Pro Gln Tyr Ile Lys Asp 
                405                 410                 415     
His Pro Ile Tyr Tyr Ala Gly Pro Ala Lys Thr Pro Ala Gly Tyr Pro 
            420                 425                 430         
Ser Gly Ser Leu Gly Pro Thr Thr Ala Gly Arg Met Asp Ser Tyr Val 
        435                 440                 445             
Asp Leu Leu Gln Ser His Gly Gly Ser Met Ile Met Leu Ala Lys Gly 
    450                 455                 460                 
Asn Arg Ser Gln Gln Val Thr Asp Ala Cys His Lys His Gly Gly Phe 
465                 470                 475                 480 
Tyr Leu Gly Ser Ile Gly Gly Pro Ala Ala Val Leu Ala Gln Gln Ser 
                485                 490                 495     
Ile Lys His Leu Glu Cys Val Ala Tyr Pro Glu Leu Gly Met Glu Ala 
            500                 505                 510         
Ile Trp Lys Ile Glu Val Glu Asp Phe Pro Ala Phe Ile Leu Val Asp 
        515                 520                 525             
Asp Lys Gly Asn Asp Phe Phe Gln Gln Ile Val Asn Lys Gln Cys Ala 
    530                 535                 540                 
Asn Cys Thr Lys 
545             

<210> 2
<211> 8074
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..8074
<223> /organism="Artificial sequence"
      /note="Sequence of plasmid pSUC223"
      /mol_type="unassigned DNA"

<400> 2
gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca       60

cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct      120

cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat      180

tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttaa      240

ttaaaggagg tgcacgcatt atggagacca ctacgatacg atagctgcgt tgttgttgaa      300

ggggtttctt aaggttgttt tcgttgaagg taaatattgg tcgtttttgt gcagcatatt      360

gtcctctaga tgcaaactct gcaggtccat ttgcagtaaa gtgagttgcc tctcgaagaa      420

tcattaattt cgtataaccg tcactattaa agtcagaaaa taaattctgt cgtagacaat      480

gttaccataa tgttcttgtc cattttgcat acactttaaa tattcatttg atttctcagg      540

gttcatgatc ataataaatt gcgcattcgc aaggcggtag tattataatg gggtccatca      600

ttctgtagca agaagttaca gtacgctgtt caagcgttaa acaagataag taatctcgaa      660

tgaaacattc atatttcgca tgagccaaca tacagttgct gagtaatctt cattgcgctt      720

atttatcggc attgagattg taaaggaagt aaaacgcatt tttgcagatc tgttctctta      780

tgtattttta atcgtccttg tatggaagta tcaaagggga cgttcttcac ctccttggaa      840

ggatcccagc gccagtaggg ttgttgagct tagtaaaaat gtgcgcacca caagcctaca      900

tgactccacg tcacatgaaa ccacaccgtg gggccttgtt gcgctaggaa taggatatgc      960

gacgaagacg cttctgctta gtaaccacac cacattttca gggggtcgat ctgcttgctt     1020

cctttactgt cacgagcggc ccataatcgc gctttttttt taaaaggcgc gagacagcaa     1080

acaggaagct cgggtttcaa ccttcggagt ggtcgcagat ctggagactg gatctttaca     1140

atacagtaag gcaagccacc atctgcttct taggtgcatg cgacggtatc cacgtgcaga     1200

acaacatagt ctgaagaagg gggggaggag catgttcatt ctctgtagca gtaagagctt     1260

ggtgataatg accaaaactg gagtctcgaa atcatataaa tagacaatat attttcacac     1320

aatgagattt gtagtacagt tctattctct ctcttgcata aataagaaat tcatcaagaa     1380

cttggtttga tatttcacca acacacacaa aaaacagtac ttcactaaat ttacacacaa     1440

aacaaaatgt ccaacaagcc tttcatctac caagctccat tcccaatggg taaggacaac     1500

actgaatact acttgttgac ttctgactac gtttccgttg ctgatttcga tggtgaaacc     1560

atcttgaagg ttgaaccaga agccttgact ttgttggctc aacaagcctt ccacgatgct     1620

tctttcatgt tgcgtccagc tcaccaaaag caagttgctg ccattttgca cgacccagaa     1680

gcctccgaaa acgacaaata cgttgctttg caattcttga gaaactctga aattgctgcc     1740

aagggtgtct taccaacttg tcaagacact ggtactgcca tcattgtcgg taagaagggt     1800

caaagagtct ggaccggtgg tggtgacgaa gaaactctat ccaagggtgt ttacaacact     1860

tacattgaag ataatttacg ttactctcaa aatgctgctt tggacatgta caaggaagtc     1920

aacactggta ccaacttgcc agctcaaatc gacttatacg ctgttgacgg tgacgaatac     1980

aagttcttgt gtgttgccaa gggtggtggt tctgctaaca agacctactt gtaccaagaa     2040

accaaggctt tgttgactcc aggtaaattg aagaacttct tggtcgaaaa gatgagaact     2100

ttgggtactg ctgcttgtcc accataccac attgctttcg ttatcggtgg tacttccgct     2160

gaaaccaact tgaaaaccgt caaattggct tccgctcact actacgatga attgccaact     2220

gaaggtaacg aacacggtca agccttcaga gatgtccaat tggaacaaga attgttggaa     2280

gaagctcaaa aattaggttt gggtgctcaa tttggtggta aatactttgc tcacgatatc     2340

agagttatca gattaccaag acatggtgct tcttgtccag ttggtatggg tgtttcctgt     2400

tctgctgaca gaaacatcaa ggccaagatc aacagagaag gtatctggat tgaaaaattg     2460

gaacacaacc caggtcaata catcccacaa gaattgagac aagctggtga aggtgaagct     2520

gtcaaggttg acttgaacag accaatgaag gaaatcttgg ctcaattatc tcaataccca     2580

gtttccacca gattatcttt gaccggtacc atcattgtcg gtcgtgacat tgctcatgcc     2640

aagttgaagg aattgattga tgctggtaag gaattgcctc aatacatcaa ggaccatcca     2700

atctactacg ctggtccagc caagacccca gctggttacc catctggttc tttgggtcca     2760

accaccgctg gtagaatgga ctcttacgtt gacttgctac aatctcacgg tggttccatg     2820

atcatgttgg ctaagggtaa cagatctcaa caagtcaccg atgcttgtca caagcacggt     2880

ggtttctatt tgggttccat tggtggtcca gctgctgtct tggctcaaca atctatcaag     2940

cacttggaat gtgttgctta cccagaattg ggtatggaag ccatctggaa gattgaagtc     3000

gaagatttcc cagctttcat cttagtcgat gacaagggta acgacttctt ccaacaaatt     3060

gtcaacaagc aatgtgccaa ctgtaccaag taagcgtacg ataaagcaat cttgatgagg     3120

ataatgattt ttttttgaat atacataaat actaccgttt ttctgctaga ttttgtgaag     3180

acgtaaataa gtacatatta ctttttaagc caagacaaga ttaagcatta actttaccct     3240

tttctcttct aagtttcaat actagttatc actgtttaaa agttatggcg agaacgtcgg     3300

cggttaaaat atattaccct gaacgtggtg aattgaagtt ctaggatggt ttaaagattt     3360

ttcctttttg ggaaataagt aaacaatata ttgctgcctt ggcgcgcccc ttccgggccc     3420

acgcgtggcc ggccgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgaattcta     3480

ccgttcgtat aatgtatgct atacgaagtt atagatctgt ttagcttgcc tcgtccccgc     3540

cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc     3600

gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat     3660

aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct     3720

cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg     3780

ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata     3840

tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac     3900

catgggtaag gaaaagactc acgtttcgag gccgcgatta aattccaaca tggatgctga     3960

tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg     4020

attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc     4080

caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc     4140

gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc     4200

cggcaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga     4260

tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa     4320

cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga     4380

tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat     4440

gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga     4500

taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat     4560

cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc     4620

attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca     4680

gtttcatttg atgctcgatg agtttttcta atcagtactg acaataaaaa gattcttgtt     4740

ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat     4800

gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt     4860

gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg     4920

attcgatact aacgccgcca tccagtgtcg aaaacgagct cataacttcg tataatgtat     4980

gctatacgaa cggtagaatt cgaatcagat ccactagtgg cctatgcggc cgcgatggga     5040

cgtcagcact gtacttgttt ttgcgactag attgtaaatc attctttatt taatctcttt     5100

ctttaactac tgcttaaagt ataatttggt ccgtagttta ataactatac taagcgtaac     5160

aatgcatact gacattataa gcctgaacat tacgagttta agttgtatgt aggcgttctg     5220

taagaggtta ctgcgtaaat tatcaacgaa tgcattggtg tatttgcgaa agctacttct     5280

tttaacaagt atttacataa gaataatggt gatctgctca actgatttgg tgataactct     5340

aactttttta gcaacaattt aaaagataat tcgaacatat ataacagtag gaagaatttg     5400

tgtacgtcaa attaagataa tttagcatta ccaaagttat taacctaaac ataaaatata     5460

tatgagacac atgtggaaat cgtatgaaac aactgttatg aaactgacaa gaatgaatat     5520

atagagtaag ctccgcttgt aaagaggaat cacttaagtg tataaatgtc tcgacgatta     5580

ctttagatcc aagattgatg attgatatta ctctgtaata cttaagctct tttaatagct     5640

cactgttgta ttacgggctc gagtaatacc ggaattcact ggccgtcgtt ttacaacgtc     5700

gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg     5760

ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc     5820

tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac     5880

accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc     5940

gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt     6000

acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac     6060

cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga     6120

taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta     6180

tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat     6240

aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc     6300

ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga     6360

aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca     6420

acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt     6480

ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg     6540

gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc     6600

atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata     6660

acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt     6720

tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag     6780

ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca     6840

aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg     6900

aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg     6960

ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag     7020

atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg     7080

aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag     7140

accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga     7200

tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt     7260

tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc     7320

tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc     7380

cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac     7440

caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac     7500

cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt     7560

cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct     7620

gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat     7680

acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt     7740

atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg     7800

cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt     7860

gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt     7920

tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg     7980

tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg     8040

agcgcagcga gtcagtgagc gaggaagcgg aaga                                 8074


<210> 3
<211> 452
<212> PRT
<213> Artificial sequence

<220> 
<223> Modified protein sequence of YEL047c encoding FRD1 (E.C. 1.3.1.6,
      UniProt accession number P32614)

<400> 3
Met Asn Glu Leu Val Asn Lys Tyr Asn Ile Pro Val Thr Ile Leu Glu 
1               5                   10                  15      
Lys Ala Ser Ser Ile Gly Gly Asn Ser Ile Lys Ala Ser Ser Gly Ile 
            20                  25                  30          
Asn Gly Ala Cys Thr Glu Thr Gln Arg His Phe His Ile Glu Asp Ser 
        35                  40                  45              
Pro Arg Leu Phe Glu Asp Asp Thr Ile Lys Ser Ala Lys Gly Lys Gly 
    50                  55                  60                  
Val Gln Glu Leu Met Ala Lys Leu Ala Asn Asp Ser Pro Leu Ala Ile 
65                  70                  75                  80  
Glu Trp Leu Lys Asn Glu Phe Asp Leu Lys Leu Asp Leu Leu Ala Gln 
                85                  90                  95      
Leu Gly Gly His Ser Val Ala Arg Thr His Arg Ser Ser Gly Lys Leu 
            100                 105                 110         
Pro Pro Gly Phe Glu Ile Val Ser Ala Leu Ser Asn Asn Leu Lys Lys 
        115                 120                 125             
Leu Ala Glu Thr Lys Pro Glu Leu Val Lys Ile Asn Leu Asp Ser Lys 
    130                 135                 140                 
Val Val Asp Ile His Glu Lys Asp Gly Ser Ile Ser Ala Val Val Tyr 
145                 150                 155                 160 
Glu Asp Lys Asn Gly Glu Lys His Met Val Ser Ala Asn Asp Val Val 
                165                 170                 175     
Phe Cys Ser Gly Gly Phe Gly Phe Ser Lys Glu Met Leu Lys Glu Tyr 
            180                 185                 190         
Ala Pro Glu Leu Val Asn Leu Pro Thr Thr Asn Gly Gln Gln Thr Thr 
        195                 200                 205             
Gly Asp Gly Gln Arg Leu Leu Gln Lys Leu Gly Ala Asp Leu Ile Asp 
    210                 215                 220                 
Met Asp Gln Ile Gln Val His Pro Thr Gly Phe Ile Asp Pro Asn Asp 
225                 230                 235                 240 
Arg Ser Ser Ser Trp Lys Phe Leu Ala Ala Glu Ser Leu Arg Gly Leu 
                245                 250                 255     
Gly Gly Ile Leu Leu Asn Pro Ile Thr Gly Arg Arg Phe Val Asn Glu 
            260                 265                 270         
Leu Thr Thr Arg Asp Val Val Thr Ala Ala Ile Gln Lys Val Cys Pro 
        275                 280                 285             
Gln Glu Asp Asn Arg Ala Leu Leu Val Met Gly Glu Lys Met Tyr Thr 
    290                 295                 300                 
Asp Leu Lys Asn Asn Leu Asp Phe Tyr Met Phe Lys Lys Leu Val Gln 
305                 310                 315                 320 
Lys Leu Thr Leu Ser Gln Val Val Ser Glu Tyr Asn Leu Pro Ile Thr 
                325                 330                 335     
Val Ala Gln Leu Cys Glu Glu Leu Gln Thr Tyr Ser Ser Phe Thr Thr 
            340                 345                 350         
Lys Ala Asp Pro Leu Gly Arg Thr Val Ile Leu Asn Glu Phe Gly Ser 
        355                 360                 365             
Asp Val Thr Pro Glu Thr Val Val Phe Ile Gly Glu Val Thr Pro Val 
    370                 375                 380                 
Val His Phe Thr Met Gly Gly Ala Arg Ile Asn Val Lys Ala Gln Val 
385                 390                 395                 400 
Ile Gly Lys Asn Asp Glu Arg Leu Leu Lys Gly Leu Tyr Ala Ala Gly 
                405                 410                 415     
Glu Val Ser Gly Gly Val His Gly Ala Asn Arg Leu Gly Gly Ser Ser 
            420                 425                 430         
Leu Leu Glu Cys Val Val Phe Gly Arg Thr Ala Ala Glu Ser Ile Ala 
        435                 440                 445             
Asn Asp Arg Lys 
    450         

<210> 4
<211> 2260
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..2260
<223> /organism="Artificial sequence"
      /note="Synthetic TDH3p-FRD1-TDH3t gene"
      /mol_type="unassigned DNA"

<400> 4
ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt acatgcccaa aatagggggc      60

gggttacaca gaatatataa catcgtaggt gtctgggtga acagtttatt cctggcatcc     120

actaaatata atggagcccg ctttttaagc tggcatccag aaaaaaaaag aatcccagca     180

ccaaaatatt gttttcttca ccaaccatca gttcataggt ccattctctt agcgcaacta     240

cagagaacag gggcacaaac aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc     300

tgcctggagt aaatgatgac acaaggcaat tgacccacgc atgtatctat ctcattttct     360

tacaccttct attaccttct gctctctctg atttggaaaa agctgaaaaa aaaggttgaa     420

accagttccc tgaaattatt cccctacttg actaataagt atataaagac ggtaggtatt     480

gattgtaatt ctgtaaatct atttcttaaa cttcttaaat tctactttta tagttagtct     540

tttttttagt tttaaaacac caagaactta gtttcgaata aacacacata aacaaacaaa     600

atgaacgaat tagtcaacaa atacaacatt ccagtcacca ttttggaaaa ggcttcttcc     660

attggtggta actccatcaa ggcttcctcc ggtatcaacg gtgcttgtac cgaaactcaa     720

agacatttcc acattgaaga ttctccaaga ttgtttgaag atgacaccat caaatctgcc     780

aagggtaagg gtgtccaaga attgatggcc aagttggcta acgactctcc attggccatt     840

gaatggttga aaaatgaatt cgacttgaaa ttggacttgt tggctcaatt aggtggtcac     900

tccgttgcca gaactcaccg ttcttctggt aagttgccac caggtttcga aattgtttct     960

gctttgtcca acaacttgaa gaaattggct gaaaccaagc cagaattggt caagatcaac    1020

ttggactcca aggttgtcga tatccacgaa aaggacggtt ccatctctgc tgttgtttac    1080

gaagataaga acggtgaaaa gcacatggtt tctgctaacg atgttgtctt ttgttctggt    1140

ggtttcggtt tctccaagga aatgttgaag gaatacgctc cagaattggt caacttgcca    1200

accaccaacg gtcaacaaac cactggtgat ggtcaaagat tactacaaaa gttgggtgct    1260

gacttgattg acatggacca aatccaagtt cacccaactg gtttcattga cccaaacgac    1320

cgttcttctt cctggaaatt cttggctgct gaatctttga gaggtttggg tggtatcttg    1380

ttgaacccaa tcactggtag aagatttgtc aatgaattga ccaccagaga tgtcgttacc    1440

gctgccatcc aaaaggtctg tccacaagaa gataacagag ctttgttggt catgggtgaa    1500

aagatgtaca ctgacttgaa gaacaacttg gacttctaca tgttcaagaa gttggttcaa    1560

aagttgactt tatctcaagt tgtttctgaa tacaacttgc caatcactgt tgctcaatta    1620

tgtgaagaat tgcaaactta ctcctctttc accaccaagg ctgacccttt gggtagaacc    1680

gttatcttga acgaattcgg ttctgatgtc actccagaaa ctgttgtttt catcggtgaa    1740

gtcactccag ttgtccactt caccatgggt ggtgccagaa tcaacgtcaa ggctcaagtt    1800

atcggtaaga acgatgaaag attattgaag ggtttatacg ctgctggtga agtctctggt    1860

ggtgtccacg gtgctaacag attaggtggt tcctctctat tggaatgtgt tgttttcggt    1920

agaactgctg ctgaatccat tgccaacgac cgcaagtaag gtgaatttac tttaaatctt    1980

gcatttaaat aaattttctt tttatagctt tatgacttag tttcaattta tatactattt    2040

taatgacatt ttcgattcat tgattgaaag ctttgtgttt tttcttgatg cgctattgca    2100

ttgttcttgt ctttttcgcc acatgtaata tctgtagtag atacctgata cattgtggat    2160

gctgagtgaa attttagtta ataatggagg cgctcttaat aattttgggg atattggctt    2220

ttttttttaa agtttacaaa tgaatttttt ccgccaggat                          2260


<210> 5
<211> 84
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..84
<223> /organism="Artificial sequence"
      /note="Primer 1 (TDH3p FW with PDC6 5' overhang)"
      /mol_type="unassigned DNA"

<400> 5
gtcctctttt atatacagta taaataaaaa acccacgtaa tatagcaaaa acatattgcc     60

aacaaattag tcaaaaaatt agcc                                            84


<210> 6
<211> 82
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..82
<223> /organism="Artificial sequence"
      /note="Primer 2 (TDH3t REV with overhang to pSUC228 Cre-1)"
      /mol_type="unassigned DNA"

<400> 6
cgctatactg atttaaatat aacttcgtat agcatacatt atacgaacgg tactcgaggt     60

cgaatcctgg cggaaaaaat tc                                              82


<210> 7
<211> 86
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..86
<223> /organism="Artificial sequence"
      /note="Primer 3 (pSUC228 Cre-1 FW with overhang TDH3t)"
      /mol_type="unassigned DNA"

<400> 7
cttaataatt ttggggatat tggctttttt ttttaaagtt tacaaatgaa ttttttccgc     60

caggattcga cctcgagtac cgttcg                                          86


<210> 8
<211> 21
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..21
<223> /organism="Artificial sequence"
      /note="Primer 4 (DBC-03373, pSUC228 Cre-1 REV)"
      /mol_type="unassigned DNA"

<400> 8
gcaatttcgg ctatacgtaa c                                              21


<210> 9
<211> 4780
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..4780
<223> /organism="Artificial sequence"
      /note="Sequence of plasmid pSUC228"
      /mol_type="unassigned DNA"

<400> 9
aaatagcttg ccttgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc       60

ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt      120

tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg      180

cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca      240

gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg      300

ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac      360

atcacatccg aacataaaca accatgggta ccactcttga cgacacggct taccggtacc      420

gcaccagtgt cccgggggac gccgaggcca tcgaggcact ggatgggtcc ttcaccaccg      480

acaccgtctt ccgcgtcacc gccaccgggg acggcttcac cctgcgggag gtgccggtgg      540

acccgcccct gaccaaggtg ttccccgacg acgaatcgga cgacgaatcg gacgacgggg      600

aggacggcga cccggactcc cggacgttcg tcgcgtacgg ggacgacggc gacctggcgg      660

gcttcgtggt cgtctcgtac tccggctgga accgccggct gaccgtcgag gacatcgagg      720

tcgccccgga gcaccggggg cacggggtcg ggcgcgcgtt gatggggctc gcgacggagt      780

tcgcccgcga gcggggcgcc gggcacctct ggctggaggt caccaacgtc aacgcaccgg      840

cgatccacgc gtaccggcgg atggggttca ccctctgcgg cctggacacc gccctgtacg      900

acggcaccgc ctcggacggc gagcaggcgc tctacatgag catgccctgc ccctaatcag      960

tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat     1020

tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat     1080

catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg     1140

aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag tgtcgattta     1200

aatctagtac ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct     1260

ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac     1320

tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg     1380

gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga     1440

taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt     1500

tgatctatta acagatatat aaatgcaaaa actgcataac cactttaact aatactttca     1560

acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt     1620

taatatacct ctatacttta acgtcaagga gaaaaaaccc cggattctag aactagtgga     1680

tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgaggg gcagagccga     1740

tcctgtacac tttacttaaa accattatct gagtgttaaa tgtccaattt actgaccgta     1800

caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg     1860

atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc     1920

gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa     1980

cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact     2040

atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtccgggct gccacgacca     2100

agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc     2160

ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca     2220

ctcatggaaa atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct     2280

tataacaccc tgttacgtat agccgaaatt gcggcgcgcc ggtacctctt aattaactgg     2340

cctcatgggc cttccgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc     2400

attaacatgg tcatagctgt ttccttgcgt attgggcgct ctccgcttcc tcgctcactg     2460

actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc taatgagcaa aaggccagca     2520

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc     2580

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata     2640

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc     2700

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc     2760

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga     2820

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc     2880

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag     2940

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag     3000

aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag     3060

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca     3120

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga     3180

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat     3240

cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga     3300

gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg     3360

tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga     3420

gggcttacca tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc     3480

agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac     3540

tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc     3600

agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc     3660

gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc     3720

catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt     3780

ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc     3840

atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg     3900

tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag     3960

cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat     4020

cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc     4080

atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa     4140

aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta     4200

ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa     4260

aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc     4320

gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa     4380

taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt     4440

ggccgctaca gggcgctccc attcgccatt caggctgcgc aactgttggg aagggcgttt     4500

cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat     4560

taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc     4620

gcgacgtaat acgactcact atagggcgaa ttggcggaag gccgtcaagg cctaggcgcg     4680

ccatgagctc ggcgcgccgc ggccgcaggc ctggcgccca gctggggccc gtcgacctcg     4740

agtaccgttc gtataatgta tgctatacga agttatattt                           4780


<210> 10
<211> 21
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..21
<223> /organism="Artificial sequence"
      /note="Primer 5 (DBC-03374, pSUC225 Cre-2 FW)"
      /mol_type="unassigned DNA"

<400> 10
cgttcactca tggaaaatag c                                              21


<210> 11
<211> 85
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..85
<223> /organism="Artificial sequence"
      /note="Primer 6 (pSUC225 Cre-2 REV with PDC6 3' overhang)"
      /mol_type="unassigned DNA"

<400> 11
gtattcaaac tgtgtaagtt tatttatttg caacaataat tcgtttgagt acactactaa     60

tggccggatc ctaccgttcg tatag                                           85


<210> 12
<211> 1139
<212> PRT
<213> Artificial Sequence

<220> 
<223> Glycosomal Trypanosoma brucei fumarate reductase (FRDg) amino
      acid sequence lacking 3 aa C-terminal targeting signal.

<400> 12
Met Val Asp Gly Arg Ser Ser Ala Ser Ile Val Ala Val Asp Pro Glu 
1               5                   10                  15      
Arg Ala Ala Arg Glu Arg Asp Ala Ala Ala Arg Ala Leu Leu Gln Asp 
            20                  25                  30          
Ser Pro Leu His Thr Thr Met Gln Tyr Ala Thr Ser Gly Leu Glu Leu 
        35                  40                  45              
Thr Val Pro Tyr Ala Leu Lys Val Val Ala Ser Ala Asp Thr Phe Asp 
    50                  55                  60                  
Arg Ala Lys Glu Val Ala Asp Glu Val Leu Arg Cys Ala Trp Gln Leu 
65                  70                  75                  80  
Ala Asp Thr Val Leu Asn Ser Phe Asn Pro Asn Ser Glu Val Ser Leu 
                85                  90                  95      
Val Gly Arg Leu Pro Val Gly Gln Lys His Gln Met Ser Ala Pro Leu 
            100                 105                 110         
Lys Arg Val Met Ala Cys Cys Gln Arg Val Tyr Asn Ser Ser Ala Gly 
        115                 120                 125             
Cys Phe Asp Pro Ser Thr Ala Pro Val Ala Lys Ala Leu Arg Glu Ile 
    130                 135                 140                 
Ala Leu Gly Lys Glu Arg Asn Asn Ala Cys Leu Glu Ala Leu Thr Gln 
145                 150                 155                 160 
Ala Cys Thr Leu Pro Asn Ser Phe Val Ile Asp Phe Glu Ala Gly Thr 
                165                 170                 175     
Ile Ser Arg Lys His Glu His Ala Ser Leu Asp Leu Gly Gly Val Ser 
            180                 185                 190         
Lys Gly Tyr Ile Val Asp Tyr Val Ile Asp Asn Ile Asn Ala Ala Gly 
        195                 200                 205             
Phe Gln Asn Val Phe Phe Asp Trp Gly Gly Asp Cys Arg Ala Ser Gly 
    210                 215                 220                 
Met Asn Ala Arg Asn Thr Pro Trp Val Val Gly Ile Thr Arg Pro Pro 
225                 230                 235                 240 
Ser Leu Asp Met Leu Pro Asn Pro Pro Lys Glu Ala Ser Tyr Ile Ser 
                245                 250                 255     
Val Ile Ser Leu Asp Asn Glu Ala Leu Ala Thr Ser Gly Asp Tyr Glu 
            260                 265                 270         
Asn Leu Ile Tyr Thr Ala Asp Asp Lys Pro Leu Thr Cys Thr Tyr Asp 
        275                 280                 285             
Trp Lys Gly Lys Glu Leu Met Lys Pro Ser Gln Ser Asn Ile Ala Gln 
    290                 295                 300                 
Val Ser Val Lys Cys Tyr Ser Ala Met Tyr Ala Asp Ala Leu Ala Thr 
305                 310                 315                 320 
Ala Cys Phe Ile Lys Arg Asp Pro Ala Lys Val Arg Gln Leu Leu Asp 
                325                 330                 335     
Gly Trp Arg Tyr Val Arg Asp Thr Val Arg Asp Tyr Arg Val Tyr Val 
            340                 345                 350         
Arg Glu Asn Glu Arg Val Ala Lys Met Phe Glu Ile Ala Thr Glu Asp 
        355                 360                 365             
Ala Glu Met Arg Lys Arg Arg Ile Ser Asn Thr Leu Pro Ala Arg Val 
    370                 375                 380                 
Ile Val Val Gly Gly Gly Leu Ala Gly Leu Ser Ala Ala Ile Glu Ala 
385                 390                 395                 400 
Ala Gly Cys Gly Ala Gln Val Val Leu Met Glu Lys Glu Ala Lys Leu 
                405                 410                 415     
Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly Ile Asn Gly Trp Gly Thr 
            420                 425                 430         
Arg Ala Gln Ala Lys Ala Ser Ile Val Asp Gly Gly Lys Tyr Phe Glu 
        435                 440                 445             
Arg Asp Thr Tyr Lys Ser Gly Ile Gly Gly Asn Thr Asp Pro Ala Leu 
    450                 455                 460                 
Val Lys Thr Leu Ser Met Lys Ser Ala Asp Ala Ile Gly Trp Leu Thr 
465                 470                 475                 480 
Ser Leu Gly Val Pro Leu Thr Val Leu Ser Gln Leu Gly Gly His Ser 
                485                 490                 495     
Arg Lys Arg Thr His Arg Ala Pro Asp Lys Lys Asp Gly Thr Pro Leu 
            500                 505                 510         
Pro Ile Gly Phe Thr Ile Met Lys Thr Leu Glu Asp His Val Arg Gly 
        515                 520                 525             
Asn Leu Ser Gly Arg Ile Thr Ile Met Glu Asn Cys Ser Val Thr Ser 
    530                 535                 540                 
Leu Leu Ser Glu Thr Lys Glu Arg Pro Asp Gly Thr Lys Gln Ile Arg 
545                 550                 555                 560 
Val Thr Gly Val Glu Phe Thr Gln Ala Gly Ser Gly Lys Thr Thr Ile 
                565                 570                 575     
Leu Ala Asp Ala Val Ile Leu Ala Thr Gly Gly Phe Ser Asn Asp Lys 
            580                 585                 590         
Thr Ala Asp Ser Leu Leu Arg Glu His Ala Pro His Leu Val Asn Phe 
        595                 600                 605             
Pro Thr Thr Asn Gly Pro Trp Ala Thr Gly Asp Gly Val Lys Leu Ala 
    610                 615                 620                 
Gln Arg Leu Gly Ala Gln Leu Val Asp Met Asp Lys Val Gln Leu His 
625                 630                 635                 640 
Pro Thr Gly Leu Ile Asn Pro Lys Asp Pro Ala Asn Pro Thr Lys Phe 
                645                 650                 655     
Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly Gly Val Leu Leu Asn Lys 
            660                 665                 670         
Gln Gly Lys Arg Phe Val Asn Glu Leu Asp Leu Arg Ser Val Val Ser 
        675                 680                 685             
Lys Ala Ile Met Glu Gln Gly Ala Glu Tyr Pro Gly Ser Gly Gly Ser 
    690                 695                 700                 
Met Phe Ala Tyr Cys Val Leu Asn Ala Ala Ala Gln Lys Leu Phe Gly 
705                 710                 715                 720 
Val Ser Ser His Glu Phe Tyr Trp Lys Lys Met Gly Leu Phe Val Lys 
                725                 730                 735     
Ala Asp Thr Met Arg Asp Leu Ala Ala Leu Ile Gly Cys Pro Val Glu 
            740                 745                 750         
Ser Val Gln Gln Thr Leu Glu Glu Tyr Glu Arg Leu Ser Ile Ser Gln 
        755                 760                 765             
Arg Ser Cys Pro Ile Thr Arg Lys Ser Val Tyr Pro Cys Val Leu Gly 
    770                 775                 780                 
Thr Lys Gly Pro Tyr Tyr Val Ala Phe Val Thr Pro Ser Ile His Tyr 
785                 790                 795                 800 
Thr Met Gly Gly Cys Leu Ile Ser Pro Ser Ala Glu Ile Gln Met Lys 
                805                 810                 815     
Asn Thr Ser Ser Arg Ala Pro Leu Ser His Ser Asn Pro Ile Leu Gly 
            820                 825                 830         
Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val His Gly Gly Asn Arg 
        835                 840                 845             
Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val Phe Gly Arg Ile Ala 
    850                 855                 860                 
Gly Asp Arg Ala Ser Thr Ile Leu Gln Arg Lys Ser Ser Ala Leu Ser 
865                 870                 875                 880 
Phe Lys Val Trp Thr Thr Val Val Leu Arg Glu Val Arg Glu Gly Gly 
                885                 890                 895     
Val Tyr Gly Ala Gly Ser Arg Val Leu Arg Phe Asn Leu Pro Gly Ala 
            900                 905                 910         
Leu Gln Arg Ser Gly Leu Ser Leu Gly Gln Phe Ile Ala Ile Arg Gly 
        915                 920                 925             
Asp Trp Asp Gly Gln Gln Leu Ile Gly Tyr Tyr Ser Pro Ile Thr Leu 
    930                 935                 940                 
Pro Asp Asp Leu Gly Met Ile Asp Ile Leu Ala Arg Ser Asp Lys Gly 
945                 950                 955                 960 
Thr Leu Arg Glu Trp Ile Ser Ala Leu Glu Pro Gly Asp Ala Val Glu 
                965                 970                 975     
Met Lys Ala Cys Gly Gly Leu Val Ile Glu Arg Arg Leu Ser Asp Lys 
            980                 985                 990         
His Phe Val Phe Met Gly His Ile Ile Asn Lys Leu Cys Leu Ile Ala 
        995                 1000                1005            
Gly Gly Thr Gly Val Ala Pro Met Leu Gln Ile Ile Lys Ala Ala Phe 
    1010                1015                1020                
Met Lys Pro Phe Ile Asp Thr Leu Glu Ser Val His Leu Ile Tyr Ala 
1025                1030                1035                1040
Ala Glu Asp Val Thr Glu Leu Thr Tyr Arg Glu Val Leu Glu Glu Arg 
                1045                1050                1055    
Arg Arg Glu Ser Arg Gly Lys Phe Lys Lys Thr Phe Val Leu Asn Arg 
            1060                1065                1070        
Pro Pro Pro Leu Trp Thr Asp Gly Val Gly Phe Ile Asp Arg Gly Ile 
        1075                1080                1085            
Leu Thr Asn His Val Gln Pro Pro Ser Asp Asn Leu Leu Val Ala Ile 
    1090                1095                1100                
Cys Gly Pro Pro Val Met Gln Arg Ile Val Lys Ala Thr Leu Lys Thr 
1105                1110                1115                1120
Leu Gly Tyr Asn Met Asn Leu Val Arg Thr Val Asp Glu Thr Glu Pro 
                1125                1130                1135    
Ser Gly Ser 
            

<210> 13
<211> 472
<212> PRT
<213> Artificial Sequence

<220> 
<223> Rhizopus oryzae fumarase amino acid sequence, lacking the first
      23 N-terminal amino acids.

<400> 13
Met Ser Ser Ala Ser Ala Ala Leu Gln Lys Phe Arg Ala Glu Arg Asp 
1               5                   10                  15      
Thr Phe Gly Asp Leu Gln Val Pro Ala Asp Arg Tyr Trp Gly Ala Gln 
            20                  25                  30          
Thr Gln Arg Ser Leu Gln Asn Phe Asp Ile Gly Gly Pro Thr Glu Arg 
        35                  40                  45              
Met Pro Glu Pro Leu Ile Arg Ala Phe Gly Val Leu Lys Lys Ala Ala 
    50                  55                  60                  
Ala Thr Val Asn Met Thr Tyr Gly Leu Asp Pro Lys Val Gly Glu Ala 
65                  70                  75                  80  
Ile Gln Lys Ala Ala Asp Glu Val Ile Asp Gly Ser Leu Ile Asp His 
                85                  90                  95      
Phe Pro Leu Val Val Trp Gln Thr Gly Ser Gly Thr Gln Thr Lys Met 
            100                 105                 110         
Asn Val Asn Glu Val Ile Ser Asn Arg Ala Ile Glu Leu Leu Gly Gly 
        115                 120                 125             
Glu Leu Gly Ser Lys Ala Pro Val His Pro Asn Asp His Val Asn Met 
    130                 135                 140                 
Ser Gln Ser Ser Asn Asp Thr Phe Pro Thr Ala Met His Val Ala Ala 
145                 150                 155                 160 
Val Val Glu Ile His Gly Arg Leu Ile Pro Ala Leu Thr Thr Leu Arg 
                165                 170                 175     
Asp Ala Leu Gln Ala Lys Ser Ala Glu Phe Glu His Ile Ile Lys Ile 
            180                 185                 190         
Gly Arg Thr His Leu Gln Asp Ala Thr Pro Leu Thr Leu Gly Gln Glu 
        195                 200                 205             
Phe Ser Gly Tyr Thr Gln Gln Leu Thr Tyr Gly Ile Ala Arg Val Gln 
    210                 215                 220                 
Gly Thr Leu Glu Arg Leu Tyr Asn Leu Ala Gln Gly Gly Thr Ala Val 
225                 230                 235                 240 
Gly Thr Gly Leu Asn Thr Arg Lys Gly Phe Asp Ala Lys Val Ala Glu 
                245                 250                 255     
Ala Ile Ala Ser Ile Thr Gly Leu Pro Phe Lys Thr Ala Pro Asn Lys 
            260                 265                 270         
Phe Glu Ala Leu Ala Ala His Asp Ala Leu Val Glu Ala His Gly Ala 
        275                 280                 285             
Leu Asn Thr Val Ala Cys Ser Leu Met Lys Ile Ala Asn Asp Ile Arg 
    290                 295                 300                 
Tyr Leu Gly Ser Gly Pro Arg Cys Gly Leu Gly Glu Leu Ser Leu Pro 
305                 310                 315                 320 
Glu Asn Glu Pro Gly Ser Ser Ile Met Pro Gly Lys Val Asn Pro Thr 
                325                 330                 335     
Gln Cys Glu Ala Met Thr Met Val Cys Ala Gln Val Met Gly Asn Asn 
            340                 345                 350         
Thr Ala Ile Ser Val Ala Gly Ser Asn Gly Gln Phe Glu Leu Asn Val 
        355                 360                 365             
Phe Lys Pro Val Met Ile Lys Asn Leu Ile Gln Ser Ile Arg Leu Ile 
    370                 375                 380                 
Ser Asp Ala Ser Ile Ser Phe Thr Lys Asn Cys Val Val Gly Ile Glu 
385                 390                 395                 400 
Ala Asn Glu Lys Lys Ile Ser Ser Ile Met Asn Glu Ser Leu Met Leu 
                405                 410                 415     
Val Thr Ala Leu Asn Pro His Ile Gly Tyr Asp Lys Ala Ala Lys Cys 
            420                 425                 430         
Ala Lys Lys Ala His Lys Glu Gly Thr Thr Leu Lys Glu Ala Ala Leu 
        435                 440                 445             
Ser Leu Gly Tyr Leu Thr Ser Glu Glu Phe Asp Gln Trp Val Arg Pro 
    450                 455                 460                 
Glu Asp Met Ile Ser Ala Lys Asp 
465                 470         

<210> 14
<211> 538
<212> PRT
<213> Artificial Sequence

<220> 
<223> Actinobacillus succinogenes phosphoenolpyruvate carboxykinase
      amino acid sequence, with EGY to DAF modification at pos 120 -
      122.

<400> 14
Met Thr Asp Leu Asn Lys Leu Val Lys Glu Leu Asn Asp Leu Gly Leu 
1               5                   10                  15      
Thr Asp Val Lys Glu Ile Val Tyr Asn Pro Ser Tyr Glu Gln Leu Phe 
            20                  25                  30          
Glu Glu Glu Thr Lys Pro Gly Leu Glu Gly Phe Asp Lys Gly Thr Leu 
        35                  40                  45              
Thr Thr Leu Gly Ala Val Ala Val Asp Thr Gly Ile Phe Thr Gly Arg 
    50                  55                  60                  
Ser Pro Lys Asp Lys Tyr Ile Val Cys Asp Glu Thr Thr Lys Asp Thr 
65                  70                  75                  80  
Val Trp Trp Asn Ser Glu Ala Ala Lys Asn Asp Asn Lys Pro Met Thr 
                85                  90                  95      
Gln Glu Thr Trp Lys Ser Leu Arg Glu Leu Val Ala Lys Gln Leu Ser 
            100                 105                 110         
Gly Lys Arg Leu Phe Val Val Asp Ala Phe Cys Gly Ala Ser Glu Lys 
        115                 120                 125             
His Arg Ile Gly Val Arg Met Val Thr Glu Val Ala Trp Gln Ala His 
    130                 135                 140                 
Phe Val Lys Asn Met Phe Ile Arg Pro Thr Asp Glu Glu Leu Lys Asn 
145                 150                 155                 160 
Phe Lys Ala Asp Phe Thr Val Leu Asn Gly Ala Lys Cys Thr Asn Pro 
                165                 170                 175     
Asn Trp Lys Glu Gln Gly Leu Asn Ser Glu Asn Phe Val Ala Phe Asn 
            180                 185                 190         
Ile Thr Glu Gly Ile Gln Leu Ile Gly Gly Thr Trp Tyr Gly Gly Glu 
        195                 200                 205             
Met Lys Lys Gly Met Phe Ser Met Met Asn Tyr Phe Leu Pro Leu Lys 
    210                 215                 220                 
Gly Val Ala Ser Met His Cys Ser Ala Asn Val Gly Lys Asp Gly Asp 
225                 230                 235                 240 
Val Ala Ile Phe Phe Gly Leu Ser Gly Thr Gly Lys Thr Thr Leu Ser 
                245                 250                 255     
Thr Asp Pro Lys Arg Gln Leu Ile Gly Asp Asp Glu His Gly Trp Asp 
            260                 265                 270         
Glu Ser Gly Val Phe Asn Phe Glu Gly Gly Cys Tyr Ala Lys Thr Ile 
        275                 280                 285             
Asn Leu Ser Gln Glu Asn Glu Pro Asp Ile Tyr Gly Ala Ile Arg Arg 
    290                 295                 300                 
Asp Ala Leu Leu Glu Asn Val Val Val Arg Ala Asp Gly Ser Val Asp 
305                 310                 315                 320 
Phe Asp Asp Gly Ser Lys Thr Glu Asn Thr Arg Val Ser Tyr Pro Ile 
                325                 330                 335     
Tyr His Ile Asp Asn Ile Val Arg Pro Val Ser Lys Ala Gly His Ala 
            340                 345                 350         
Thr Lys Val Ile Phe Leu Thr Ala Asp Ala Phe Gly Val Leu Pro Pro 
        355                 360                 365             
Val Ser Lys Leu Thr Pro Glu Gln Thr Glu Tyr Tyr Phe Leu Ser Gly 
    370                 375                 380                 
Phe Thr Ala Lys Leu Ala Gly Thr Glu Arg Gly Val Thr Glu Pro Thr 
385                 390                 395                 400 
Pro Thr Phe Ser Ala Cys Phe Gly Ala Ala Phe Leu Ser Leu His Pro 
                405                 410                 415     
Ile Gln Tyr Ala Asp Val Leu Val Glu Arg Met Lys Ala Ser Gly Ala 
            420                 425                 430         
Glu Ala Tyr Leu Val Asn Thr Gly Trp Asn Gly Thr Gly Lys Arg Ile 
        435                 440                 445             
Ser Ile Lys Asp Thr Arg Gly Ile Ile Asp Ala Ile Leu Asp Gly Ser 
    450                 455                 460                 
Ile Glu Lys Ala Glu Met Gly Glu Leu Pro Ile Phe Asn Leu Ala Ile 
465                 470                 475                 480 
Pro Lys Ala Leu Pro Gly Val Asp Pro Ala Ile Leu Asp Pro Arg Asp 
                485                 490                 495     
Thr Tyr Ala Asp Lys Ala Gln Trp Gln Val Lys Ala Glu Asp Leu Ala 
            500                 505                 510         
Asn Arg Phe Val Lys Asn Phe Val Lys Tyr Thr Ala Asn Pro Glu Ala 
        515                 520                 525             
Ala Lys Leu Val Gly Ala Gly Pro Lys Ala 
    530                 535             

<210> 15
<211> 340
<212> PRT
<213> Artificial Sequence

<220> 
<223> Saccharomyces cerevisiae peroxisomal malate dehydrogenase (Mdh3)
      amino acid sequence, lacking the 3 C-terminal peroxisomal
      targeting sequence (SKL).

<400> 15
Met Val Lys Val Ala Ile Leu Gly Ala Ser Gly Gly Val Gly Gln Pro 
1               5                   10                  15      
Leu Ser Leu Leu Leu Lys Leu Ser Pro Tyr Val Ser Glu Leu Ala Leu 
            20                  25                  30          
Tyr Asp Ile Arg Ala Ala Glu Gly Ile Gly Lys Asp Leu Ser His Ile 
        35                  40                  45              
Asn Thr Asn Ser Ser Cys Val Gly Tyr Asp Lys Asp Ser Ile Glu Asn 
    50                  55                  60                  
Thr Leu Ser Asn Ala Gln Val Val Leu Ile Pro Ala Gly Val Pro Arg 
65                  70                  75                  80  
Lys Pro Gly Leu Thr Arg Asp Asp Leu Phe Lys Met Asn Ala Gly Ile 
                85                  90                  95      
Val Lys Ser Leu Val Thr Ala Val Gly Lys Phe Ala Pro Asn Ala Arg 
            100                 105                 110         
Ile Leu Val Ile Ser Asn Pro Val Asn Ser Leu Val Pro Ile Ala Val 
        115                 120                 125             
Glu Thr Leu Lys Lys Met Gly Lys Phe Lys Pro Gly Asn Val Met Gly 
    130                 135                 140                 
Val Thr Asn Leu Asp Leu Val Arg Ala Glu Thr Phe Leu Val Asp Tyr 
145                 150                 155                 160 
Leu Met Leu Lys Asn Pro Lys Ile Gly Gln Glu Gln Asp Lys Thr Thr 
                165                 170                 175     
Met His Arg Lys Val Thr Val Ile Gly Gly His Ser Gly Glu Thr Ile 
            180                 185                 190         
Ile Pro Ile Ile Thr Asp Lys Ser Leu Val Phe Gln Leu Asp Lys Gln 
        195                 200                 205             
Tyr Glu His Phe Ile His Arg Val Gln Phe Gly Gly Asp Glu Ile Val 
    210                 215                 220                 
Lys Ala Lys Gln Gly Ala Gly Ser Ala Thr Leu Ser Met Ala Phe Ala 
225                 230                 235                 240 
Gly Ala Lys Phe Ala Glu Glu Val Leu Arg Ser Phe His Asn Glu Lys 
                245                 250                 255     
Pro Glu Thr Glu Ser Leu Ser Ala Phe Val Tyr Leu Pro Gly Leu Lys 
            260                 265                 270         
Asn Gly Lys Lys Ala Gln Gln Leu Val Gly Asp Asn Ser Ile Glu Tyr 
        275                 280                 285             
Phe Ser Leu Pro Ile Val Leu Arg Asn Gly Ser Val Val Ser Ile Asp 
    290                 295                 300                 
Thr Ser Val Leu Glu Lys Leu Ser Pro Arg Glu Glu Gln Leu Val Asn 
305                 310                 315                 320 
Thr Ala Val Lys Glu Leu Arg Lys Asn Ile Glu Lys Gly Lys Ser Phe 
                325                 330                 335     
Ile Leu Asp Ser 
            340 

<210> 16
<211> 1180
<212> PRT
<213> Saccharomyces cerevisiae


<400> 16
Met Ser Ser Ser Lys Lys Leu Ala Gly Leu Arg Asp Asn Phe Ser Leu 
1               5                   10                  15      
Leu Gly Glu Lys Asn Lys Ile Leu Val Ala Asn Arg Gly Glu Ile Pro 
            20                  25                  30          
Ile Arg Ile Phe Arg Ser Ala His Glu Leu Ser Met Arg Thr Ile Ala 
        35                  40                  45              
Ile Tyr Ser His Glu Asp Arg Leu Ser Met His Arg Leu Lys Ala Asp 
    50                  55                  60                  
Glu Ala Tyr Val Ile Gly Glu Glu Gly Gln Tyr Thr Pro Val Gly Ala 
65                  70                  75                  80  
Tyr Leu Ala Met Asp Glu Ile Ile Glu Ile Ala Lys Lys His Lys Val 
                85                  90                  95      
Asp Phe Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ser Glu Phe 
            100                 105                 110         
Ala Asp Lys Val Val Lys Ala Gly Ile Thr Trp Ile Gly Pro Pro Ala 
        115                 120                 125             
Glu Val Ile Asp Ser Val Gly Asp Lys Val Ser Ala Arg His Leu Ala 
    130                 135                 140                 
Ala Arg Ala Asn Val Pro Thr Val Pro Gly Thr Pro Gly Pro Ile Glu 
145                 150                 155                 160 
Thr Val Gln Glu Ala Leu Asp Phe Val Asn Glu Tyr Gly Tyr Pro Val 
                165                 170                 175     
Ile Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met Arg Val Val 
            180                 185                 190         
Arg Glu Gly Asp Asp Val Ala Asp Ala Phe Gln Arg Ala Thr Ser Glu 
        195                 200                 205             
Ala Arg Thr Ala Phe Gly Asn Gly Thr Cys Phe Val Glu Arg Phe Leu 
    210                 215                 220                 
Asp Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp Asn His Gly 
225                 230                 235                 240 
Asn Val Val His Leu Phe Glu Arg Asp Cys Ser Val Gln Arg Arg His 
                245                 250                 255     
Gln Lys Val Val Glu Val Ala Pro Ala Lys Thr Leu Pro Arg Glu Val 
            260                 265                 270         
Arg Asp Ala Ile Leu Thr Asp Ala Val Lys Leu Ala Lys Val Cys Gly 
        275                 280                 285             
Tyr Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Asn Gln Asn Arg 
    290                 295                 300                 
His Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu His Thr Ile 
305                 310                 315                 320 
Thr Glu Glu Ile Thr Gly Ile Asp Ile Val Ser Ala Gln Ile Gln Ile 
                325                 330                 335     
Ala Ala Gly Ala Thr Leu Thr Gln Leu Gly Leu Leu Gln Asp Lys Ile 
            340                 345                 350         
Thr Thr Arg Gly Phe Ser Ile Gln Cys Arg Ile Thr Thr Glu Asp Pro 
        355                 360                 365             
Ser Lys Asn Phe Gln Pro Asp Thr Gly Arg Leu Glu Val Tyr Arg Ser 
    370                 375                 380                 
Ala Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Ala Tyr Ala Gly 
385                 390                 395                 400 
Ala Thr Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys Cys Ser Cys 
                405                 410                 415     
Ser Gly Ser Thr Tyr Glu Ile Val Arg Arg Lys Met Ile Arg Ala Leu 
            420                 425                 430         
Ile Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro Phe Leu Leu 
        435                 440                 445             
Thr Leu Leu Thr Asn Pro Val Phe Ile Glu Gly Thr Tyr Trp Thr Thr 
    450                 455                 460                 
Phe Ile Asp Asp Thr Pro Gln Leu Phe Gln Met Val Ser Ser Gln Asn 
465                 470                 475                 480 
Arg Ala Gln Lys Leu Leu His Tyr Leu Ala Asp Leu Ala Val Asn Gly 
                485                 490                 495     
Ser Ser Ile Lys Gly Gln Ile Gly Leu Pro Lys Leu Lys Ser Asn Pro 
            500                 505                 510         
Ser Val Pro His Leu His Asp Ala Gln Gly Asn Val Ile Asn Val Thr 
        515                 520                 525             
Lys Ser Ala Pro Pro Ser Gly Trp Arg Gln Val Leu Leu Glu Lys Gly 
    530                 535                 540                 
Pro Ser Glu Phe Ala Lys Gln Val Arg Gln Phe Asn Gly Thr Leu Leu 
545                 550                 555                 560 
Met Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala Thr Arg 
                565                 570                 575     
Val Arg Thr His Asp Leu Ala Thr Ile Ala Pro Thr Thr Ala His Ala 
            580                 585                 590         
Leu Ala Gly Ala Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr Phe Asp 
        595                 600                 605             
Val Ala Met Arg Phe Leu His Glu Asp Pro Trp Glu Arg Leu Arg Lys 
    610                 615                 620                 
Leu Arg Ser Leu Val Pro Asn Ile Pro Phe Gln Met Leu Leu Arg Gly 
625                 630                 635                 640 
Ala Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile Asp His 
                645                 650                 655     
Phe Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg Val Phe 
            660                 665                 670         
Asp Ala Leu Asn Asp Leu Glu Gln Leu Lys Val Gly Val Asn Ala Val 
        675                 680                 685             
Lys Lys Ala Gly Gly Val Val Glu Ala Thr Val Cys Tyr Ser Gly Asp 
    690                 695                 700                 
Met Leu Gln Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu Glu Val 
705                 710                 715                 720 
Val Glu Lys Ile Val Gln Met Gly Thr His Ile Leu Gly Ile Lys Asp 
                725                 730                 735     
Met Ala Gly Thr Met Lys Pro Ala Ala Ala Lys Leu Leu Ile Gly Ser 
            740                 745                 750         
Leu Arg Thr Arg Tyr Pro Asp Leu Pro Ile His Val His Ser His Asp 
        755                 760                 765             
Ser Ala Gly Thr Ala Val Ala Ser Met Thr Ala Cys Ala Leu Ala Gly 
    770                 775                 780                 
Ala Asp Val Val Asp Val Ala Ile Asn Ser Met Ser Gly Leu Thr Ser 
785                 790                 795                 800 
Gln Pro Ser Ile Asn Ala Leu Leu Ala Ser Leu Glu Gly Asn Ile Asp 
                805                 810                 815     
Thr Gly Ile Asn Val Glu His Val Arg Glu Leu Asp Ala Tyr Trp Ala 
            820                 825                 830         
Glu Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys Gly Pro 
        835                 840                 845             
Asp Pro Glu Val Tyr Gln His Glu Ile Pro Gly Gly Gln Leu Thr Asn 
    850                 855                 860                 
Leu Leu Phe Gln Ala Gln Gln Leu Gly Leu Gly Glu Gln Trp Ala Glu 
865                 870                 875                 880 
Thr Lys Arg Ala Tyr Arg Glu Ala Asn Tyr Leu Leu Gly Asp Ile Val 
                885                 890                 895     
Lys Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln Phe Met 
            900                 905                 910         
Val Ser Asn Lys Leu Thr Ser Asp Asp Ile Arg Arg Leu Ala Asn Ser 
        915                 920                 925             
Leu Asp Phe Pro Asp Ser Val Met Asp Phe Phe Glu Gly Leu Ile Gly 
    930                 935                 940                 
Gln Pro Tyr Gly Gly Phe Pro Glu Pro Leu Arg Ser Asp Val Leu Arg 
945                 950                 955                 960 
Asn Lys Arg Arg Lys Leu Thr Cys Arg Pro Gly Leu Glu Leu Glu Pro 
                965                 970                 975     
Phe Asp Leu Glu Lys Ile Arg Glu Asp Leu Gln Asn Arg Phe Gly Asp 
            980                 985                 990         
Ile Asp Glu Cys Asp Val Ala Ser Tyr Asn Met Tyr Pro Arg Val Tyr 
        995                 1000                1005            
Glu Asp Phe Gln Lys Ile Arg Glu Thr Tyr Gly Asp Leu Ser Val Leu 
    1010                1015                1020                
Pro Thr Lys Asn Phe Leu Ala Pro Ala Glu Pro Asp Glu Glu Ile Glu 
1025                1030                1035                1040
Val Thr Ile Glu Gln Gly Lys Thr Leu Ile Ile Lys Leu Gln Ala Val 
                1045                1050                1055    
Gly Asp Leu Asn Lys Lys Thr Gly Gln Arg Glu Val Tyr Phe Glu Leu 
            1060                1065                1070        
Asn Gly Glu Leu Arg Lys Ile Arg Val Ala Asp Lys Ser Gln Asn Ile 
        1075                1080                1085            
Gln Ser Val Ala Lys Pro Lys Ala Asp Val His Asp Thr His Gln Ile 
    1090                1095                1100                
Gly Ala Pro Met Ala Gly Val Ile Ile Glu Val Lys Val His Lys Gly 
1105                1110                1115                1120
Ser Leu Val Lys Lys Gly Glu Ser Ile Ala Val Leu Ser Ala Met Lys 
                1125                1130                1135    
Met Glu Met Val Val Ser Ser Pro Ala Asp Gly Gln Val Lys Asp Val 
            1140                1145                1150        
Phe Ile Lys Asp Gly Glu Ser Val Asp Ala Ser Asp Leu Leu Val Val 
        1155                1160                1165            
Leu Glu Glu Glu Thr Leu Pro Pro Ser Gln Lys Lys 
    1170                1175                1180

<210> 17
<211> 542
<212> PRT
<213> Kluyveromyces lactis


<400> 17
Met Val Ser Val Lys Ala Ser Ala Ala Glu Lys Lys Glu Phe Leu Gln 
1               5                   10                  15      
Ser Gln Ile Asp Glu Ile Glu Lys Trp Trp Ser Glu Pro Arg Trp Lys 
            20                  25                  30          
Asp Thr Lys Arg Ile Tyr Ser Ala Tyr Glu Ile Ala Lys Arg Arg Gly 
        35                  40                  45              
Ser Val Lys Pro Asn Thr Phe Pro Ser Thr Val Met Ser Gln Lys Leu 
    50                  55                  60                  
Phe Lys Ile Leu Gly Glu His Ala Lys Asn Gly Thr Val Ser Lys Thr 
65                  70                  75                  80  
Phe Gly Ala Leu Asp Pro Val Gln Val Thr Gln Met Ser Lys Tyr Leu 
                85                  90                  95      
Asp Thr Ile Tyr Val Ser Gly Trp Gln Cys Ser Ser Thr Ala Ser Thr 
            100                 105                 110         
Ser Asn Glu Pro Gly Pro Asp Leu Ala Asp Tyr Pro Met Asp Thr Val 
        115                 120                 125             
Pro Asn Lys Val Glu His Leu Phe Lys Ala Gln Gln Phe His Asp Arg 
    130                 135                 140                 
Lys Gln Trp Glu Arg Ile Cys Asp Gly Thr Ile Glu Glu Ser Glu Ile 
145                 150                 155                 160 
Ile Asp Tyr Leu Thr Pro Ile Val Ala Asp Gly Asp Ala Gly His Gly 
                165                 170                 175     
Gly Leu Thr Ala Val Phe Lys Leu Thr Lys Met Phe Ile Glu Arg Gly 
            180                 185                 190         
Ala Ala Gly Ile His Ile Glu Asp Gln Thr Ser Thr Asn Lys Lys Cys 
        195                 200                 205             
Gly His Met Ala Gly Arg Cys Val Ile Pro Val Gln Glu His Ile Asn 
    210                 215                 220                 
Arg Leu Ile Thr Cys Arg Met Ala Ala Asp Val Leu Gly Ser Asp Leu 
225                 230                 235                 240 
Ile Leu Val Ala Arg Thr Asp Ser Glu Ala Ala Thr Leu Leu Ser Ser 
                245                 250                 255     
Thr Ala Asp Ser Arg Asp His Tyr Phe Ile Leu Gly Ala Ser Asn Pro 
            260                 265                 270         
Ala Val Lys Gly Lys Pro Leu Asn Asp Leu Leu Asn Lys Ala Ile Leu 
        275                 280                 285             
Asp Gly Ala Thr Ile Asp Asp Leu Gln Thr Ile Glu Lys Glu Trp Leu 
    290                 295                 300                 
Ala Lys Ala Asp Val Lys Leu Phe His Glu Val Phe Ala Asp Ala Ala 
305                 310                 315                 320 
Lys Ala Ala Gly Lys Asp Gln Ser Val Ile Asp Gln Phe Asn Ser Lys 
                325                 330                 335     
Val Asn Pro Leu Ser Glu Thr Ser Ile Tyr Glu Met Gln Ala Leu Ala 
            340                 345                 350         
Lys Glu Leu Leu Gly Thr Glu Leu Phe Phe Asp Trp Asp Leu Pro Arg 
        355                 360                 365             
Gly Arg Glu Gly Leu Tyr Arg Tyr Gln Gly Gly Thr Gln Cys Ser Val 
    370                 375                 380                 
Met Arg Ala Arg Ala Phe Ala Pro Tyr Ala Asp Leu Cys Trp Met Glu 
385                 390                 395                 400 
Ser Asn Tyr Pro Asp Tyr Glu Gln Ala Lys Glu Phe Ala Glu Gly Val 
                405                 410                 415     
Thr Ala Lys Phe Pro Gly Lys Trp Met Ala Tyr Asn Leu Ser Pro Ser 
            420                 425                 430         
Phe Asn Trp Thr Lys Ala Met Ser Val Asp Glu Gln Glu Thr Phe Ile 
        435                 440                 445             
Gln Arg Leu Gly Asp Leu Gly Tyr Ile Trp Gln Phe Ile Thr Leu Ala 
    450                 455                 460                 
Gly Leu His Thr Ser Gly Leu Ala Ile Glu Gln Phe Ser Lys Asn Phe 
465                 470                 475                 480 
Ala Lys Leu Gly Met Lys Ala Tyr Ala Gln Asp Ile Gln Lys Lys Glu 
                485                 490                 495     
Leu Asp Asn Gly Ile Asp Met Val Lys His Gln Lys Trp Ser Gly Ala 
            500                 505                 510         
Glu Tyr Ile Asp Gly Leu Leu Arg Leu Ala Gln Gly Gly Leu Ala Ala 
        515                 520                 525             
Thr Ala Ala Met Gly Gln Gly Val Thr Glu Asp Gln Phe Lys 
    530                 535                 540         

<210> 18
<211> 551
<212> PRT
<213> Artificial Sequence

<220> 
<223> Sacharomyces cerevisiae malate synthase amino acid sequence
      (artificial) Saccharomyces cerevisiae peroxisomal malate synthase
      (Mls1) amino acid sequence, lacking the 3 C-terminal peroxisomal
      targeting

<400> 18
Met Val Lys Val Ser Leu Asp Asn Val Lys Leu Leu Val Asp Val Asp 
1               5                   10                  15      
Lys Glu Pro Phe Phe Lys Pro Ser Ser Thr Thr Val Gly Asp Ile Leu 
            20                  25                  30          
Thr Lys Asp Ala Leu Glu Phe Ile Val Leu Leu His Arg Thr Phe Asn 
        35                  40                  45              
Asn Lys Arg Lys Gln Leu Leu Glu Asn Arg Gln Val Val Gln Lys Lys 
    50                  55                  60                  
Leu Asp Ser Gly Ser Tyr His Leu Asp Phe Leu Pro Glu Thr Ala Asn 
65                  70                  75                  80  
Ile Arg Asn Asp Pro Thr Trp Gln Gly Pro Ile Leu Ala Pro Gly Leu 
                85                  90                  95      
Ile Asn Arg Ser Thr Glu Ile Thr Gly Pro Pro Leu Arg Asn Met Leu 
            100                 105                 110         
Ile Asn Ala Leu Asn Ala Pro Val Asn Thr Tyr Met Thr Asp Phe Glu 
        115                 120                 125             
Asp Ser Ala Ser Pro Thr Trp Asn Asn Met Val Tyr Gly Gln Val Asn 
    130                 135                 140                 
Leu Tyr Asp Ala Ile Arg Asn Gln Ile Asp Phe Asp Thr Pro Arg Lys 
145                 150                 155                 160 
Ser Tyr Lys Leu Asn Gly Asn Val Ala Asn Leu Pro Thr Ile Ile Val 
                165                 170                 175     
Arg Pro Arg Gly Trp His Met Val Glu Lys His Leu Tyr Val Asp Asp 
            180                 185                 190         
Glu Pro Ile Ser Ala Ser Ile Phe Asp Phe Gly Leu Tyr Phe Tyr His 
        195                 200                 205             
Asn Ala Lys Glu Leu Ile Lys Leu Gly Lys Gly Pro Tyr Phe Tyr Leu 
    210                 215                 220                 
Pro Lys Met Glu His His Leu Glu Ala Lys Leu Trp Asn Asp Val Phe 
225                 230                 235                 240 
Cys Val Ala Gln Asp Tyr Ile Gly Ile Pro Arg Gly Thr Ile Arg Ala 
                245                 250                 255     
Thr Val Leu Ile Glu Thr Leu Pro Ala Ala Phe Gln Met Glu Glu Ile 
            260                 265                 270         
Ile Tyr Gln Leu Arg Gln His Ser Ser Gly Leu Asn Cys Gly Arg Trp 
        275                 280                 285             
Asp Tyr Ile Phe Ser Thr Ile Lys Arg Leu Arg Asn Asp Pro Asn His 
    290                 295                 300                 
Ile Leu Pro Asn Arg Asn Gln Val Thr Met Thr Ser Pro Phe Met Asp 
305                 310                 315                 320 
Ala Tyr Val Lys Arg Leu Ile Asn Thr Cys His Arg Arg Gly Val His 
                325                 330                 335     
Ala Met Gly Gly Met Ala Ala Gln Ile Pro Ile Lys Asp Asp Pro Ala 
            340                 345                 350         
Ala Asn Glu Lys Ala Met Thr Lys Val Arg Asn Asp Lys Ile Arg Glu 
        355                 360                 365             
Leu Thr Asn Gly His Asp Gly Ser Trp Val Ala His Pro Ala Leu Ala 
    370                 375                 380                 
Pro Ile Cys Asn Glu Val Phe Ile Asn Met Gly Thr Pro Asn Gln Ile 
385                 390                 395                 400 
Tyr Phe Ile Pro Glu Asn Val Val Thr Ala Ala Asn Leu Leu Glu Thr 
                405                 410                 415     
Lys Ile Pro Asn Gly Glu Ile Thr Thr Glu Gly Ile Val Gln Asn Leu 
            420                 425                 430         
Asp Ile Gly Leu Gln Tyr Met Glu Ala Trp Leu Arg Gly Ser Gly Cys 
        435                 440                 445             
Val Pro Ile Asn Asn Leu Met Glu Asp Ala Ala Thr Ala Glu Val Ser 
    450                 455                 460                 
Arg Cys Gln Leu Tyr Gln Trp Val Lys His Gly Val Thr Leu Lys Asp 
465                 470                 475                 480 
Thr Gly Glu Lys Val Thr Pro Glu Leu Thr Glu Lys Ile Leu Lys Glu 
                485                 490                 495     
Gln Val Glu Arg Leu Ser Lys Ala Ser Pro Leu Gly Asp Lys Asn Lys 
            500                 505                 510         
Phe Ala Leu Ala Ala Lys Tyr Phe Leu Pro Glu Ile Arg Gly Glu Lys 
        515                 520                 525             
Phe Ser Glu Phe Leu Thr Thr Leu Leu Tyr Asp Glu Ile Val Ser Thr 
    530                 535                 540                 
Lys Ala Thr Pro Thr Asp Leu 
545                 550     

<210> 19
<211> 416
<212> PRT
<213> Aspergillus niger


<400> 19
Met Asn Val Glu Thr Ser Leu Pro Gly Ser Ser Gly Ser Asp Leu Glu 
1               5                   10                  15      
Thr Phe His His Glu Thr Lys Lys His Ala Asn His Asp Ser Gly Ile 
            20                  25                  30          
Ser Val Asn His Glu Ala Glu Ile Gly Val Asn His Thr Phe Glu Lys 
        35                  40                  45              
Pro Gly Pro Val Gly Ile Arg Glu Arg Leu Arg His Phe Thr Trp Ala 
    50                  55                  60                  
Trp Tyr Thr Leu Thr Met Ser Cys Gly Gly Leu Ala Leu Leu Ile Val 
65                  70                  75                  80  
Asn Gln Pro His Asp Phe Lys Gly Leu Lys Asp Ile Ala Arg Val Val 
                85                  90                  95      
Tyr Cys Leu Asn Leu Ala Phe Phe Val Ile Val Thr Ser Leu Met Ala 
            100                 105                 110         
Ile Arg Phe Ile Leu His Lys Asn Met Trp Glu Ser Leu Gly His Asp 
        115                 120                 125             
Arg Glu Gly Leu Phe Phe Pro Thr Phe Trp Leu Ser Ile Ala Thr Met 
    130                 135                 140                 
Ile Thr Gly Leu Tyr Lys Cys Phe Gly Asp Asp Ala Asn Glu Lys Phe 
145                 150                 155                 160 
Thr Lys Cys Leu Gln Val Leu Phe Trp Ile Tyr Cys Gly Cys Thr Met 
                165                 170                 175     
Ile Thr Ala Val Gly Gln Tyr Ser Phe Val Phe Ala Thr His Lys Tyr 
            180                 185                 190         
Glu Leu His Thr Met Met Pro Ser Trp Ile Leu Pro Ala Phe Pro Val 
        195                 200                 205             
Met Leu Ser Gly Thr Ile Ala Ser Val Ile Gly Ser Gly Gln Pro Ala 
    210                 215                 220                 
Ser Asp Gly Ile Pro Ile Ile Ile Ala Gly Ile Thr Phe Gln Gly Leu 
225                 230                 235                 240 
Gly Phe Ser Ile Ser Phe Met Met Tyr Ala His Tyr Ile Gly Arg Leu 
                245                 250                 255     
Met Glu Val Gly Leu Pro Ser Pro Glu His Arg Pro Gly Met Phe Ile 
            260                 265                 270         
Cys Val Gly Pro Pro Ala Phe Thr Ala Leu Ala Leu Val Gly Met Ala 
        275                 280                 285             
Lys Ala Leu Pro Asp Asp Phe Gln Ile Val Gly Asp Pro His Ala Val 
    290                 295                 300                 
Ile Asp Gly Arg Val Met Leu Phe Leu Ala Val Ser Ala Ala Ile Phe 
305                 310                 315                 320 
Leu Trp Ala Leu Ser Phe Trp Phe Phe Cys Ile Ala Val Val Ala Val 
                325                 330                 335     
Val Arg Ser Pro Pro Lys Gly Phe His Leu Asn Trp Phe Ala Met Val 
            340                 345                 350         
Phe Pro Asn Thr Gly Phe Thr Leu Ala Thr Ile Thr Leu Ala Asn Met 
        355                 360                 365             
Phe Glu Ser Pro Gly Val Lys Gly Val Ala Thr Ala Met Ser Leu Cys 
    370                 375                 380                 
Val Ile Ile Met Phe Ile Phe Val Leu Val Ser Ala Ile Arg Ala Val 
385                 390                 395                 400 
Ile Arg Lys Asp Ile Met Trp Pro Gly Gln Asp Glu Asp Val Ser Glu 
                405                 410                 415     



<210> 20
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /organism="Artificial Sequence"
      /note="Primer 7"
      /mol_type="unassigned DNA"

<400> 20
attgacgagg agttccacta cg                                             22


<210> 21
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..77
<223> /organism="Artificial Sequence"
      /note="Primer 8"
      /mol_type="unassigned DNA"

<400> 21
taaatataac ttcgtatagc atacattata cgaacggtac tcgaggtcga atattagtac     60

gtatttagtc ttgtagc                                                    77


<210> 22
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /organism="Artificial Sequence"
      /note="Primer 9"
      /mol_type="unassigned DNA"

<400> 22
gcaatttcgg ctatacgtaa c                                              21


<210> 23
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /organism="Artificial Sequence"
      /note="Primer 10"
      /mol_type="unassigned DNA"

<400> 23
cgttcactca tggaaaatag c                                              21


<210> 24
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..70
<223> /organism="Artificial Sequence"
      /note="Primer 11"
      /mol_type="unassigned DNA"

<400> 24
ttgaccgtgc tattgccatc actgctacaa gactaaatac gtactaatat tcgacctcga     60

gtaccgttcg                                                            70


<210> 25
<211> 71
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..71
<223> /organism="Artificial Sequence"
      /note="Primer 12"
      /mol_type="unassigned DNA"

<400> 25
tattactctt gtgaatgtta tctttgtcac ccttaactat catgatcgat cggatcctac     60

cgttcgtata g                                                          71


<210> 26
<211> 79
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..79
<223> /organism="Artificial Sequence"
      /note="Primer 13"
      /mol_type="unassigned DNA"

<400> 26
ggccggtaca taacttcgta taatgtatgc tatacgaacg gtaggatccg atcgatcatg     60

atagttaagg gtgacaaag                                                  79


<210> 27
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /organism="Artificial Sequence"
      /note="Primer 14"
      /mol_type="unassigned DNA"

<400> 27
tgttgggtgg tagtgggaag ag                                             22


<210> 28
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /organism="Artificial Sequence"
      /note="Primer 15"
      /mol_type="unassigned DNA"

<400> 28
tgtctcaaga tttcggcgtc tg                                             22


<210> 29
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..77
<223> /organism="Artificial Sequence"
      /note="Primer 16"
      /mol_type="unassigned DNA"

<400> 29
taaatataac ttcgtatagc atacattata cgaacggtac tcgaggtcga ctatacgaat     60

ggcaagtacg gtggttg                                                    77


<210> 30
<211> 76
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..76
<223> /organism="Artificial Sequence"
      /note="Primer 17"
      /mol_type="unassigned DNA"

<400> 30
ggccggtaca taacttcgta taatgtatgc tatacgaacg gtaggatccg tccttgtttt     60

atatattcat tgttcc                                                     76


<210> 31
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Primer 18"
      /mol_type="unassigned DNA"

<400> 31
gacaacaacg gcaacttcac                                                20


<210> 32
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..70
<223> /organism="Artificial Sequence"
      /note="Primer 19"
      /mol_type="unassigned DNA"

<400> 32
ggaacatagt agaaagacaa aaacaaccac cgtacttgcc attcgtatag tcgacctcga     60

gtaccgttcg                                                            70


<210> 33
<211> 71
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..71
<223> /organism="Artificial Sequence"
      /note="Primer 20"
      /mol_type="unassigned DNA"

<400> 33
tcatcattta aaaatgttat tctcttgtat ctatttctac tagatagatg cggatcctac     60

cgttcgtata g                                                          71


<210> 34
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Primer 21"
      /mol_type="unassigned DNA"

<400> 34
atcttccggt gagatcttcc                                                20


<210> 35
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..77
<223> /organism="Artificial Sequence"
      /note="Primer 22"
      /mol_type="unassigned DNA"

<400> 35
taaatataac ttcgtatagc atacattata cgaacggtac tcgaggtcga tattattggt     60

taatttttta tttgctc                                                    77


<210> 36
<211> 79
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..79
<223> /organism="Artificial Sequence"
      /note="Primer 23"
      /mol_type="unassigned DNA"

<400> 36
ggccggtaca taacttcgta taatgtatgc tatacgaacg gtaggatccg catctatcta     60

gtagaaatag atacaagag                                                  79


<210> 37
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Priimer 24"
      /mol_type="unassigned DNA"

<400> 37
ttcagtcgag gcatgaagtg                                                20


<210> 38
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..70
<223> /organism="Artificial Sequence"
      /note="Primer 25"
      /mol_type="unassigned DNA"

<400> 38
agcatcataa tattgactta ttagagcaaa taaaaaatta accaataata tcgacctcga     60

gtaccgttcg                                                            70


<210> 39
<211> 71
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..71
<223> /organism="Artificial Sequence"
      /note="Primer 26"
      /mol_type="unassigned DNA"

<400> 39
aacaaaactg atcatcattt aaaaatgtta ttctcttgta tctatttcta cggatcctac     60

cgttcgtata g                                                          71


<210> 40
<211> 560
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> NDE1

<400> 40
Met Ile Arg Gln Ser Leu Met Lys Thr Val Trp Ala Asn Ser Ser Arg 
1               5                   10                  15      
Phe Ser Leu Gln Ser Lys Ser Gly Leu Val Lys Tyr Ala Lys Asn Arg 
            20                  25                  30          
Ser Phe His Ala Ala Arg Asn Leu Leu Glu Asp Lys Lys Val Ile Leu 
        35                  40                  45              
Gln Lys Val Ala Pro Thr Thr Gly Val Val Ala Lys Gln Ser Phe Phe 
    50                  55                  60                  
Lys Arg Thr Gly Lys Phe Thr Leu Lys Ala Leu Leu Tyr Ser Ala Leu 
65                  70                  75                  80  
Ala Gly Thr Ala Tyr Val Ser Tyr Ser Leu Tyr Arg Glu Ala Asn Pro 
                85                  90                  95      
Ser Thr Gln Val Pro Gln Ser Asp Thr Phe Pro Asn Gly Ser Lys Arg 
            100                 105                 110         
Lys Thr Leu Val Ile Leu Gly Ser Gly Trp Gly Ser Val Ser Leu Leu 
        115                 120                 125             
Lys Asn Leu Asp Thr Thr Leu Tyr Asn Val Val Val Val Ser Pro Arg 
    130                 135                 140                 
Asn Tyr Phe Leu Phe Thr Pro Leu Leu Pro Ser Thr Pro Val Gly Thr 
145                 150                 155                 160 
Ile Glu Leu Lys Ser Ile Val Glu Pro Val Arg Thr Ile Ala Arg Arg 
                165                 170                 175     
Ser His Gly Glu Val His Tyr Tyr Glu Ala Glu Ala Tyr Asp Val Asp 
            180                 185                 190         
Pro Glu Asn Lys Thr Ile Lys Val Lys Ser Ser Ala Lys Asn Asn Asp 
        195                 200                 205             
Tyr Asp Leu Asp Leu Lys Tyr Asp Tyr Leu Val Val Gly Val Gly Ala 
    210                 215                 220                 
Gln Pro Asn Thr Phe Gly Thr Pro Gly Val Tyr Glu Tyr Ser Ser Phe 
225                 230                 235                 240 
Leu Lys Glu Ile Ser Asp Ala Gln Glu Ile Arg Leu Lys Ile Met Ser 
                245                 250                 255     
Ser Ile Glu Lys Ala Ala Ser Leu Ser Pro Lys Asp Pro Glu Arg Ala 
            260                 265                 270         
Arg Leu Leu Ser Phe Val Val Val Gly Gly Gly Pro Thr Gly Val Glu 
        275                 280                 285             
Phe Ala Ala Glu Leu Arg Asp Tyr Val Asp Gln Asp Leu Arg Lys Trp 
    290                 295                 300                 
Met Pro Glu Leu Ser Lys Glu Ile Lys Val Thr Leu Val Glu Ala Leu 
305                 310                 315                 320 
Pro Asn Ile Leu Asn Met Phe Asp Lys Tyr Leu Val Asp Tyr Ala Gln 
                325                 330                 335     
Asp Leu Phe Lys Glu Glu Lys Ile Asp Leu Arg Leu Lys Thr Met Val 
            340                 345                 350         
Lys Lys Val Asp Ala Thr Thr Ile Thr Ala Lys Thr Gly Asp Gly Asp 
        355                 360                 365             
Ile Glu Asn Ile Pro Tyr Gly Val Leu Val Trp Ala Thr Gly Asn Ala 
    370                 375                 380                 
Pro Arg Glu Val Ser Lys Asn Leu Met Thr Lys Leu Glu Glu Gln Asp 
385                 390                 395                 400 
Ser Arg Arg Gly Leu Leu Ile Asp Asn Lys Leu Gln Leu Leu Gly Ala 
                405                 410                 415     
Lys Gly Ser Ile Phe Ala Ile Gly Asp Cys Thr Phe His Pro Gly Leu 
            420                 425                 430         
Phe Pro Thr Ala Gln Val Ala His Gln Glu Gly Glu Tyr Leu Ala Gln 
        435                 440                 445             
Tyr Phe Lys Lys Ala Tyr Lys Ile Asp Gln Leu Asn Trp Lys Met Thr 
    450                 455                 460                 
His Ala Lys Asp Asp Ser Glu Val Ala Arg Leu Lys Asn Gln Ile Val 
465                 470                 475                 480 
Lys Thr Gln Ser Gln Ile Glu Asp Phe Lys Tyr Asn His Lys Gly Ala 
                485                 490                 495     
Leu Ala Tyr Ile Gly Ser Asp Lys Ala Ile Ala Asp Leu Ala Val Gly 
            500                 505                 510         
Glu Ala Lys Tyr Arg Leu Ala Gly Ser Phe Thr Phe Leu Phe Trp Lys 
        515                 520                 525             
Ser Ala Tyr Leu Ala Met Cys Leu Ser Phe Arg Asn Arg Val Leu Val 
    530                 535                 540                 
Ala Met Asp Trp Ala Lys Val Tyr Phe Leu Gly Arg Asp Ser Ser Ile 
545                 550                 555                 560 



<210> 41
<211> 545
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> NDE2

<400> 41
Met Leu Pro Arg Leu Gly Phe Ala Arg Thr Ala Arg Ser Ile His Arg 
1               5                   10                  15      
Phe Lys Met Thr Gln Ile Ser Lys Pro Phe Phe His Ser Thr Glu Val 
            20                  25                  30          
Gly Lys Pro Gly Pro Gln Gln Lys Leu Ser Lys Ser Tyr Thr Ala Val 
        35                  40                  45              
Phe Lys Lys Trp Phe Val Arg Gly Leu Lys Leu Thr Phe Tyr Thr Thr 
    50                  55                  60                  
Leu Ala Gly Thr Leu Tyr Val Ser Tyr Glu Leu Tyr Lys Glu Ser Asn 
65                  70                  75                  80  
Pro Pro Lys Gln Val Pro Gln Ser Thr Ala Phe Ala Asn Gly Leu Lys 
                85                  90                  95      
Lys Lys Glu Leu Val Ile Leu Gly Thr Gly Trp Gly Ala Ile Ser Leu 
            100                 105                 110         
Leu Lys Lys Leu Asp Thr Ser Leu Tyr Asn Val Thr Val Val Ser Pro 
        115                 120                 125             
Arg Ser Phe Phe Leu Phe Thr Pro Leu Leu Pro Ser Thr Pro Val Gly 
    130                 135                 140                 
Thr Ile Glu Met Lys Ser Ile Val Glu Pro Val Arg Ser Ile Ala Arg 
145                 150                 155                 160 
Arg Thr Pro Gly Glu Val His Tyr Ile Glu Ala Glu Ala Leu Asp Val 
                165                 170                 175     
Asp Pro Lys Ala Lys Lys Val Met Val Gln Ser Val Ser Glu Asp Glu 
            180                 185                 190         
Tyr Phe Val Ser Ser Leu Ser Tyr Asp Tyr Leu Val Val Ser Val Gly 
        195                 200                 205             
Ala Lys Thr Thr Thr Phe Asn Ile Pro Gly Val Tyr Gly Asn Ala Asn 
    210                 215                 220                 
Phe Leu Lys Glu Ile Glu Asp Ala Gln Asn Ile Arg Met Lys Leu Met 
225                 230                 235                 240 
Lys Thr Ile Glu Gln Ala Ser Ser Phe Pro Val Asn Asp Pro Glu Arg 
                245                 250                 255     
Lys Arg Leu Leu Thr Phe Val Val Val Gly Gly Gly Pro Thr Gly Val 
            260                 265                 270         
Glu Phe Ala Ala Glu Leu Gln Asp Tyr Ile Asn Gln Asp Leu Arg Lys 
        275                 280                 285             
Trp Met Pro Asp Leu Ser Lys Glu Met Lys Val Ile Leu Ile Glu Ala 
    290                 295                 300                 
Leu Pro Asn Ile Leu Asn Met Phe Asp Lys Thr Leu Ile Lys Tyr Ala 
305                 310                 315                 320 
Glu Asp Leu Phe Ala Arg Asp Glu Ile Asp Leu Gln Val Asn Thr Ala 
                325                 330                 335     
Val Lys Val Val Glu Pro Thr Tyr Ile Arg Thr Leu Gln Asn Gly Gln 
            340                 345                 350         
Thr Asn Thr Asp Ile Glu Tyr Gly Met Leu Val Trp Ala Thr Gly Asn 
        355                 360                 365             
Glu Pro Ile Asp Phe Ser Lys Thr Leu Met Ser Arg Ile Pro Glu Gln 
    370                 375                 380                 
Thr Asn Arg Arg Gly Leu Leu Ile Asn Asp Lys Leu Glu Leu Leu Gly 
385                 390                 395                 400 
Ser Glu Asn Ser Ile Tyr Ala Ile Gly Asp Cys Thr Ala His Thr Gly 
                405                 410                 415     
Phe Phe Pro Thr Ala Gln Val Ala His Gln Glu Gly Glu Tyr Leu Ala 
            420                 425                 430         
Lys Ile Leu Asp Lys Lys Leu Gln Ile Glu Gln Leu Glu Trp Asp Met 
        435                 440                 445             
Leu Asn Ser Thr Asp Glu Thr Glu Val Ser Arg Leu Gln Lys Glu Val 
    450                 455                 460                 
Asn Leu Arg Lys Ser Lys Leu Asp Lys Phe Asn Tyr Lys His Met Gly 
465                 470                 475                 480 
Ala Leu Ala Tyr Ile Gly Ser Glu Thr Ala Ile Ala Asp Leu His Met 
                485                 490                 495     
Gly Asp Ser Ser Tyr Gln Leu Lys Gly Met Phe Ala Phe Leu Phe Trp 
            500                 505                 510         
Lys Ser Ala Tyr Leu Ala Met Cys Leu Ser Ile Arg Asn Arg Ile Leu 
        515                 520                 525             
Ile Ala Met Asp Trp Thr Lys Val Tyr Phe Leu Gly Arg Asp Ser Ser 
    530                 535                 540                 
Val 
545 

<210> 42
<211> 649
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> GUT2

<400> 42
Met Phe Ser Val Thr Arg Arg Arg Ala Ala Gly Ala Ala Ala Ala Met 
1               5                   10                  15      
Ala Thr Ala Thr Gly Thr Leu Tyr Trp Met Thr Ser Gln Gly Asp Arg 
            20                  25                  30          
Pro Leu Val His Asn Asp Pro Ser Tyr Met Val Gln Phe Pro Thr Ala 
        35                  40                  45              
Ala Pro Pro Gln Val Ser Arg Arg Asp Leu Leu Asp Arg Leu Ala Lys 
    50                  55                  60                  
Thr His Gln Phe Asp Val Leu Ile Ile Gly Gly Gly Ala Thr Gly Thr 
65                  70                  75                  80  
Gly Cys Ala Leu Asp Ala Ala Thr Arg Gly Leu Asn Val Ala Leu Val 
                85                  90                  95      
Glu Lys Gly Asp Phe Ala Ser Gly Thr Ser Ser Lys Ser Thr Lys Met 
            100                 105                 110         
Ile His Gly Gly Val Arg Tyr Leu Glu Lys Ala Phe Trp Glu Phe Ser 
        115                 120                 125             
Lys Ala Gln Leu Asp Leu Val Ile Glu Ala Leu Asn Glu Arg Lys His 
    130                 135                 140                 
Leu Ile Asn Thr Ala Pro His Leu Cys Thr Val Leu Pro Ile Leu Ile 
145                 150                 155                 160 
Pro Ile Tyr Ser Thr Trp Gln Val Pro Tyr Ile Tyr Met Gly Cys Lys 
                165                 170                 175     
Phe Tyr Asp Phe Phe Ala Gly Ser Gln Asn Leu Lys Lys Ser Tyr Leu 
            180                 185                 190         
Leu Ser Lys Ser Ala Thr Val Glu Lys Ala Pro Met Leu Thr Thr Asp 
        195                 200                 205             
Asn Leu Lys Ala Ser Leu Val Tyr His Asp Gly Ser Phe Asn Asp Ser 
    210                 215                 220                 
Arg Leu Asn Ala Thr Leu Ala Ile Thr Ala Val Glu Asn Gly Ala Thr 
225                 230                 235                 240 
Val Leu Asn Tyr Val Glu Val Gln Lys Leu Ile Lys Asp Pro Thr Ser 
                245                 250                 255     
Gly Lys Val Ile Gly Ala Glu Ala Arg Asp Val Glu Thr Asn Glu Leu 
            260                 265                 270         
Val Arg Ile Asn Ala Lys Cys Val Val Asn Ala Thr Gly Pro Tyr Ser 
        275                 280                 285             
Asp Ala Ile Leu Gln Met Asp Arg Asn Pro Ser Gly Leu Pro Asp Ser 
    290                 295                 300                 
Pro Leu Asn Asp Asn Ser Lys Ile Lys Ser Thr Phe Asn Gln Ile Ala 
305                 310                 315                 320 
Val Met Asp Pro Lys Met Val Ile Pro Ser Ile Gly Val His Ile Val 
                325                 330                 335     
Leu Pro Ser Phe Tyr Cys Pro Lys Asp Met Gly Leu Leu Asp Val Arg 
            340                 345                 350         
Thr Ser Asp Gly Arg Val Met Phe Phe Leu Pro Trp Gln Gly Lys Val 
        355                 360                 365             
Leu Ala Gly Thr Thr Asp Ile Pro Leu Lys Gln Val Pro Glu Asn Pro 
    370                 375                 380                 
Met Pro Thr Glu Ala Asp Ile Gln Asp Ile Leu Lys Glu Leu Gln His 
385                 390                 395                 400 
Tyr Ile Glu Phe Pro Val Lys Arg Glu Asp Val Leu Ser Ala Trp Ala 
                405                 410                 415     
Gly Val Arg Pro Leu Val Arg Asp Pro Arg Thr Ile Pro Ala Asp Gly 
            420                 425                 430         
Lys Lys Gly Ser Ala Thr Gln Gly Val Val Arg Ser His Phe Leu Phe 
        435                 440                 445             
Thr Ser Asp Asn Gly Leu Ile Thr Ile Ala Gly Gly Lys Trp Thr Thr 
    450                 455                 460                 
Tyr Arg Gln Met Ala Glu Glu Thr Val Asp Lys Val Val Glu Val Gly 
465                 470                 475                 480 
Gly Phe His Asn Leu Lys Pro Cys His Thr Arg Asp Ile Lys Leu Ala 
                485                 490                 495     
Gly Ala Glu Glu Trp Thr Gln Asn Tyr Val Ala Leu Leu Ala Gln Asn 
            500                 505                 510         
Tyr His Leu Ser Ser Lys Met Ser Asn Tyr Leu Val Gln Asn Tyr Gly 
        515                 520                 525             
Thr Arg Ser Ser Ile Ile Cys Glu Phe Phe Lys Glu Ser Met Glu Asn 
    530                 535                 540                 
Lys Leu Pro Leu Ser Leu Ala Asp Lys Glu Asn Asn Val Ile Tyr Ser 
545                 550                 555                 560 
Ser Glu Glu Asn Asn Leu Val Asn Phe Asp Thr Phe Arg Tyr Pro Phe 
                565                 570                 575     
Thr Ile Gly Glu Leu Lys Tyr Ser Met Gln Tyr Glu Tyr Cys Arg Thr 
            580                 585                 590         
Pro Leu Asp Phe Leu Leu Arg Arg Thr Arg Phe Ala Phe Leu Asp Ala 
        595                 600                 605             
Lys Glu Ala Leu Asn Ala Val His Ala Thr Val Lys Val Met Gly Asp 
    610                 615                 620                 
Glu Phe Asn Trp Ser Glu Lys Lys Arg Gln Trp Glu Leu Glu Lys Thr 
625                 630                 635                 640 
Val Asn Phe Ile Lys Thr Phe Gly Val 
                645                 

<210> 43
<211> 4946
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..4946
<223> /organism="Artificial Sequence"
      /note="pSUC227"
      /mol_type="unassigned DNA"

<400> 43
aaatgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc       60

atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc      120

catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg      180

cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta      240

gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa      300

aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa      360

aagactcacg tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat      420

aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag      480

cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca      540

gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat      600

tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg caaaacagca      660

ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg      720

ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta      780

tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt      840

gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg      900

ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt      960

gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac     1020

caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg     1080

ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg     1140

ctcgatgagt ttttctaatc agtactgaca ataaaaagat tcttgttttc aagaacttgt     1200

catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt     1260

atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa     1320

tatcatgcgt caatcgtatg tgaatgctgg tcgctatact gatttaaata taacttcgta     1380

tagcatacat tatacgaacg gtactcgagg tcgacgggcc ccagctgggc gccaggcctg     1440

cggccgcggc gcgccgagct catggcgcgc ctaggccttg acggccttcc gccaattcgc     1500

cctatagtga gtcgtattac gtcgcgctca ctggccgtcg ttttacaacg tcgtgactgg     1560

gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg     1620

cgtaatagcg aagaggcccg caccgaaacg cccttcccaa cagttgcgca gcctgaatgg     1680

cgaatgggag cgccctgtag cggccactca accctatctc ggtctattct tttgatttat     1740

aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta     1800

acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt     1860

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag     1920

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca     1980

tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc     2040

agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat     2100

cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc     2160

aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg     2220

gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc     2280

agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat     2340

aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga     2400

gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc     2460

ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc     2520

aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt     2580

aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc     2640

tggctggttt attgctgata aatctggagc cggtgagcgt ggttctcgcg gtatcattgc     2700

agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca     2760

ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca     2820

ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt     2880

ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta     2940

acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg     3000

agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc     3060

ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag     3120

cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa     3180

gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc     3240

cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc     3300

gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta     3360

caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag     3420

aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct     3480

tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga     3540

gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc     3600

ggccttttta cggttcctgg ccttttgctg gccttttgct cattaggcac cccaggcttt     3660

acccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggagagcgc ccaatacgca     3720

aggaaacagc tatgaccatg ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg     3780

ggcagtgagc ggaaggccca tgaggccagt taattaagag gtaccggcgc gccgcaattt     3840

cggctatacg taacagggtg ttataagcaa tccccagaaa tgccagatta cgtatatcct     3900

ggcagcgatc gctattttcc atgagtgaac gaacctggtc gaaatcagtg cgttcgaacg     3960

ctagagcctg ttttgcacgt tcaccggcat caacgttttc ttttcggatc cgccgcataa     4020

ccagtgaaac agcattgctg tcacttggtc gtggcagccc ggaccgacga tgaagcatgt     4080

ttagctggcc caaatgttgc tggatagttt ttactgccag accgcgcgcc tgaagatata     4140

gaagataatc gcgaacatct tcaggttctg cgggaaacca tttccggtta ttcaacttgc     4200

accatgccgc ccacgaccgg caaacggaca gaagcatttt ccaggtatgc tcagaaaacg     4260

cctggcgatc cctgaacatg tccatcaggt tcttgcgaac ctcatcactc gttgcatcga     4320

ccggtaatgc aggcaaattt tggtgtacgg tcagtaaatt ggacatttaa cactcagata     4380

atggttttaa gtaaagtgta caggatcggc tctgcccctc gacggtatcg ataagcttga     4440

tatcgaattc ctgcagcccg ggggatccac tagttctaga atccggggtt ttttctcctt     4500

gacgttaaag tatagaggta tattaacaat tttttgttga tacttttatt acatttgaat     4560

aagaagtaat acaaaccgaa aatgttgaaa gtattagtta aagtggttat gcagtttttg     4620

catttatata tctgttaata gatcaaaaat catcgcttcg ctgattaatt accccagaaa     4680

taaggctaaa aaactaatcg cattatcatc ctatggttgt taatttgatt cgttcatttg     4740

aaggtttgtg gggccaggtt actgccaatt tttcctcttc ataaccataa aagctagtat     4800

tgtagaatct ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt tcaggaacgc     4860

gaccggtgaa gacgaggacg cacggaggag agtcttcctt cggagggctg tcacccgctc     4920

ggcggcttct aatccgtact agattt                                          4946


<210> 44
<211> 3654
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..3654
<223> /organism="Artificial Sequence"
      /note="pSUC225"
      /mol_type="unassigned DNA"

<400> 44
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt      180

gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt      240

gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg      300

acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca      360

aggcctaggc gcgccatgag ctcggccggc cttcgttcac tcatggaaaa tagcgatcgc      420

tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata      480

gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta      540

atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc      600

ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg      660

aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc      720

cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc      780

gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga      840

gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc      900

tggaccaatg taaatattgt catgaactat atccgtaccc tggatagtga aacaggggca      960

atggtgcgcc tgctggaaga tggcgattag ccattaacgc gtaaatgatt gctataatta     1020

tttgatattt atggtgacat atgagaaagg atttcaacat cgacggaaaa tatgtagtgc     1080

tgtctgtaag cactaatatt cagtcgccag ccgtcattgt cactgtaaag ctgagcgata     1140

gaatgcctga tattgactca atatccgttg cgtttcctgt caaaagtatg cgtagtgctg     1200

aacatttcgt gatgaatgcc accgaggaag aagcacggcg cggttttgct taaagtgatg     1260

tctgagtttg gcgaactctt gggtaaggtt ggaattgtcg acctcgagtc atgtaattag     1320

ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag     1380

ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa     1440

cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca     1500

ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcggcc     1560

ggtacataac ttcgtataat gtatgctata cgaacggtag gatccgaatt cgggccccag     1620

ctgggcgcca tttaaatagg cctggcgcgc cgcggccgcg gccggccggt acctcttaat     1680

taactggcct catgggcctt ccgctcactg cccgctttcc agtcgggaaa cctgtcgtgc     1740

cagctgcatt aacatggtca tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg     1800

ctcactgact cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag     1860

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc     1920

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag     1980

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     2040

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     2100

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     2160

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt     2220

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca     2280

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca     2340

ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag     2400

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca     2460

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg     2520

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa     2580

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta     2640

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag     2700

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga     2760

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagaa ccacgctcac     2820

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc     2880

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta     2940

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac     3000

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat     3060

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa     3120

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg     3180

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag     3240

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc     3300

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct     3360

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat     3420

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg     3480

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc     3540

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta     3600

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac           3654


<210> 45
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Primer 27"
      /mol_type="unassigned DNA"

<400> 45
acctatagag ccgggcagag                                                20


<210> 46
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..29
<223> /organism="Artificial Sequence"
      /note="Primer 28"
      /mol_type="unassigned DNA"

<400> 46
ctttatatta tcaatatttg tgtttgtgg                                      29


<210> 47
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..70
<223> /organism="Artificial Sequence"
      /note="Primer 29"
      /mol_type="unassigned DNA"

<400> 47
tatattgtac accccccccc tccacaaaca caaatattga taatataaag tcgacctcga     60

gtaccgttcg                                                            70


<210> 48
<211> 71
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..71
<223> /organism="Artificial Sequence"
      /note="Primer 30"
      /mol_type="unassigned DNA"

<400> 48
cctcgaaaaa agtgggggaa agtatgatat gttatctttc tccaataaat cggatcctac     60

cgttcgtata g                                                          71


<210> 49
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..29
<223> /organism="Artificial Sequence"
      /note="Primer 31"
      /mol_type="unassigned DNA"

<400> 49
atttattgga gaaagataac atatcatac                                      29


<210> 50
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Primer 32"
      /mol_type="unassigned DNA"

<400> 50
tggtgtgctt gcagtcattg                                                20


<210> 51
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /organism="Artificial Sequence"
      /note="Primer 33"
      /mol_type="unassigned DNA"

<400> 51
cgcagcagca agtaactgtg ac                                             22


<210> 52
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /organism="Artificial Sequence"
      /note="Primer 34"
      /mol_type="unassigned DNA"

<400> 52
tgataaggaa ggggagcgaa gg                                             22


<210> 53
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..70
<223> /organism="Artificial Sequence"
      /note="Primer 35"
      /mol_type="unassigned DNA"

<400> 53
agattcaatt ctctttccct ttccttttcc ttcgctcccc ttccttatca tcgacctcga     60

gtaccgttcg                                                            70


<210> 54
<211> 75
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..75
<223> /organism="Artificial Sequence"
      /note="Primer 36"
      /mol_type="unassigned DNA"

<400> 54
ctgtagtaat gttactagta gtagttgtag aacttgtgta taatgataaa ttgcggatcc     60

taccgttcgt atagc                                                      75


<210> 55
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..24
<223> /organism="Artificial Sequence"
      /note="Primer 37"
      /mol_type="unassigned DNA"

<400> 55
cacaagttct acaactacta ctag                                           24


<210> 56
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..22
<223> /organism="Artificial Sequence"
      /note="Primer 38"
      /mol_type="unassigned DNA"

<400> 56
tttccttggc ggcatcgaaa tc                                             22


<210> 57
<211> 391
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> GPD1

<400> 57
Met Ser Ala Ala Ala Asp Arg Leu Asn Leu Thr Ser Gly His Leu Asn 
1               5                   10                  15      
Ala Gly Arg Lys Arg Ser Ser Ser Ser Val Ser Leu Lys Ala Ala Glu 
            20                  25                  30          
Lys Pro Phe Lys Val Thr Val Ile Gly Ser Gly Asn Trp Gly Thr Thr 
        35                  40                  45              
Ile Ala Lys Val Val Ala Glu Asn Cys Lys Gly Tyr Pro Glu Val Phe 
    50                  55                  60                  
Ala Pro Ile Val Gln Met Trp Val Phe Glu Glu Glu Ile Asn Gly Glu 
65                  70                  75                  80  
Lys Leu Thr Glu Ile Ile Asn Thr Arg His Gln Asn Val Lys Tyr Leu 
                85                  90                  95      
Pro Gly Ile Thr Leu Pro Asp Asn Leu Val Ala Asn Pro Asp Leu Ile 
            100                 105                 110         
Asp Ser Val Lys Asp Val Asp Ile Ile Val Phe Asn Ile Pro His Gln 
        115                 120                 125             
Phe Leu Pro Arg Ile Cys Ser Gln Leu Lys Gly His Val Asp Ser His 
    130                 135                 140                 
Val Arg Ala Ile Ser Cys Leu Lys Gly Phe Glu Val Gly Ala Lys Gly 
145                 150                 155                 160 
Val Gln Leu Leu Ser Ser Tyr Ile Thr Glu Glu Leu Gly Ile Gln Cys 
                165                 170                 175     
Gly Ala Leu Ser Gly Ala Asn Ile Ala Thr Glu Val Ala Gln Glu His 
            180                 185                 190         
Trp Ser Glu Thr Thr Val Ala Tyr His Ile Pro Lys Asp Phe Arg Gly 
        195                 200                 205             
Glu Gly Lys Asp Val Asp His Lys Val Leu Lys Ala Leu Phe His Arg 
    210                 215                 220                 
Pro Tyr Phe His Val Ser Val Ile Glu Asp Val Ala Gly Ile Ser Ile 
225                 230                 235                 240 
Cys Gly Ala Leu Lys Asn Val Val Ala Leu Gly Cys Gly Phe Val Glu 
                245                 250                 255     
Gly Leu Gly Trp Gly Asn Asn Ala Ser Ala Ala Ile Gln Arg Val Gly 
            260                 265                 270         
Leu Gly Glu Ile Ile Arg Phe Gly Gln Met Phe Phe Pro Glu Ser Arg 
        275                 280                 285             
Glu Glu Thr Tyr Tyr Gln Glu Ser Ala Gly Val Ala Asp Leu Ile Thr 
    290                 295                 300                 
Thr Cys Ala Gly Gly Arg Asn Val Lys Val Ala Arg Leu Met Ala Thr 
305                 310                 315                 320 
Ser Gly Lys Asp Ala Trp Glu Cys Glu Lys Glu Leu Leu Asn Gly Gln 
                325                 330                 335     
Ser Ala Gln Gly Leu Ile Thr Cys Lys Glu Val His Glu Trp Leu Glu 
            340                 345                 350         
Thr Cys Gly Ser Val Glu Asp Phe Pro Leu Phe Glu Ala Val Tyr Gln 
        355                 360                 365             
Ile Val Tyr Asn Asn Tyr Pro Met Lys Asn Leu Pro Asp Met Ile Glu 
    370                 375                 380                 
Glu Leu Asp Leu His Glu Asp 
385                 390     

<210> 58
<211> 392
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> GPD2

<400> 58
Met Thr Ala His Thr Asn Ile Lys Gln His Lys His Cys His Glu Asp 
1               5                   10                  15      
His Pro Ile Arg Arg Ser Asp Ser Ala Val Ser Ile Val His Leu Lys 
            20                  25                  30          
Arg Ala Pro Phe Lys Val Thr Val Ile Gly Ser Gly Asn Trp Gly Thr 
        35                  40                  45              
Thr Ile Ala Lys Val Ile Ala Glu Asn Thr Glu Leu His Ser His Ile 
    50                  55                  60                  
Phe Glu Pro Glu Val Arg Met Trp Val Phe Asp Glu Lys Ile Gly Asp 
65                  70                  75                  80  
Glu Asn Leu Thr Asp Ile Ile Asn Thr Arg His Gln Asn Val Lys Tyr 
                85                  90                  95      
Leu Pro Asn Ile Asp Leu Pro His Asn Leu Val Ala Asp Pro Asp Leu 
            100                 105                 110         
Leu His Ser Ile Lys Gly Ala Asp Ile Leu Val Phe Asn Ile Pro His 
        115                 120                 125             
Gln Phe Leu Pro Asn Ile Val Lys Gln Leu Gln Gly His Val Ala Pro 
    130                 135                 140                 
His Val Arg Ala Ile Ser Cys Leu Lys Gly Phe Glu Leu Gly Ser Lys 
145                 150                 155                 160 
Gly Val Gln Leu Leu Ser Ser Tyr Val Thr Asp Glu Leu Gly Ile Gln 
                165                 170                 175     
Cys Gly Ala Leu Ser Gly Ala Asn Leu Ala Pro Glu Val Ala Lys Glu 
            180                 185                 190         
His Trp Ser Glu Thr Thr Val Ala Tyr Gln Leu Pro Lys Asp Tyr Gln 
        195                 200                 205             
Gly Asp Gly Lys Asp Val Asp His Lys Ile Leu Lys Leu Leu Phe His 
    210                 215                 220                 
Arg Pro Tyr Phe His Val Asn Val Ile Asp Asp Val Ala Gly Ile Ser 
225                 230                 235                 240 
Ile Ala Gly Ala Leu Lys Asn Val Val Ala Leu Ala Cys Gly Phe Val 
                245                 250                 255     
Glu Gly Met Gly Trp Gly Asn Asn Ala Ser Ala Ala Ile Gln Arg Leu 
            260                 265                 270         
Gly Leu Gly Glu Ile Ile Lys Phe Gly Arg Met Phe Phe Pro Glu Ser 
        275                 280                 285             
Lys Val Glu Thr Tyr Tyr Gln Glu Ser Ala Gly Val Ala Asp Leu Ile 
    290                 295                 300                 
Thr Thr Cys Ser Gly Gly Arg Asn Val Lys Val Ala Thr Tyr Met Ala 
305                 310                 315                 320 
Lys Thr Gly Lys Ser Ala Leu Glu Ala Glu Lys Glu Leu Leu Asn Gly 
                325                 330                 335     
Gln Ser Ala Gln Gly Ile Ile Thr Cys Arg Glu Val His Glu Trp Leu 
            340                 345                 350         
Gln Thr Cys Glu Leu Thr Gln Glu Phe Pro Leu Phe Glu Ala Val Tyr 
        355                 360                 365             
Gln Ile Val Tyr Asn Asn Val Arg Met Glu Asp Leu Pro Glu Met Ile 
    370                 375                 380                 
Glu Glu Leu Asp Ile Asp Asp Glu 
385                 390         

<210> 59
<211> 348
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> ADH1

<400> 59
Met Ser Ile Pro Glu Thr Gln Lys Gly Val Ile Phe Tyr Glu Ser His 
1               5                   10                  15      
Gly Lys Leu Glu Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys Ala Asn 
            20                  25                  30          
Glu Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu 
        35                  40                  45              
His Ala Trp His Gly Asp Trp Pro Leu Pro Val Lys Leu Pro Leu Val 
    50                  55                  60                  
Gly Gly His Glu Gly Ala Gly Val Val Val Gly Met Gly Glu Asn Val 
65                  70                  75                  80  
Lys Gly Trp Lys Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly 
                85                  90                  95      
Ser Cys Met Ala Cys Glu Tyr Cys Glu Leu Gly Asn Glu Ser Asn Cys 
            100                 105                 110         
Pro His Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln 
        115                 120                 125             
Tyr Ala Thr Ala Asp Ala Val Gln Ala Ala His Ile Pro Gln Gly Thr 
    130                 135                 140                 
Asp Leu Ala Gln Val Ala Pro Ile Leu Cys Ala Gly Ile Thr Val Tyr 
145                 150                 155                 160 
Lys Ala Leu Lys Ser Ala Asn Leu Met Ala Gly His Trp Val Ala Ile 
                165                 170                 175     
Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys 
            180                 185                 190         
Ala Met Gly Tyr Arg Val Leu Gly Ile Asp Gly Gly Glu Gly Lys Glu 
        195                 200                 205             
Glu Leu Phe Arg Ser Ile Gly Gly Glu Val Phe Ile Asp Phe Thr Lys 
    210                 215                 220                 
Glu Lys Asp Ile Val Gly Ala Val Leu Lys Ala Thr Asp Gly Gly Ala 
225                 230                 235                 240 
His Gly Val Ile Asn Val Ser Val Ser Glu Ala Ala Ile Glu Ala Ser 
                245                 250                 255     
Thr Arg Tyr Val Arg Ala Asn Gly Thr Thr Val Leu Val Gly Met Pro 
            260                 265                 270         
Ala Gly Ala Lys Cys Cys Ser Asp Val Phe Asn Gln Val Val Lys Ser 
        275                 280                 285             
Ile Ser Ile Val Gly Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu 
    290                 295                 300                 
Ala Leu Asp Phe Phe Ala Arg Gly Leu Val Lys Ser Pro Ile Lys Val 
305                 310                 315                 320 
Val Gly Leu Ser Thr Leu Pro Glu Ile Tyr Glu Lys Met Glu Lys Gly 
                325                 330                 335     
Gln Ile Val Gly Arg Tyr Val Val Asp Thr Ser Lys 
            340                 345             

<210> 60
<211> 277
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> ADH2

<400> 60
Met Ser Ile Pro Glu Thr Gln Lys Ala Ile Ile Phe Tyr Glu Ser Asn 
1               5                   10                  15      
Gly Lys Leu Glu His Lys Asp Ile Pro Val Pro Lys Pro Lys Pro Asn 
            20                  25                  30          
Glu Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu 
        35                  40                  45              
His Ala Trp His Gly Asp Trp Pro Leu Pro Thr Lys Leu Pro Leu Val 
    50                  55                  60                  
Gly Gly His Glu Gly Ala Gly Val Val Val Ala Ile Gly Asp Asn Val 
65                  70                  75                  80  
Arg Gly Trp Lys Val Gly Asp Leu Ala Gly Ile Lys Trp Leu Asn Ser 
                85                  90                  95      
Ser Cys Thr Gly His Trp Val Ala Ile Ser Gly Ala Ala Gly Gly Leu 
            100                 105                 110         
Gly Ser Leu Ala Val Gln Tyr Ala Lys Ala Met Gly Tyr Arg Val Leu 
        115                 120                 125             
Gly Ile Asp Gly Gly Pro Gly Lys Glu Glu Leu Phe Thr Ser Leu Gly 
    130                 135                 140                 
Gly Glu Val Phe Ile Asp Phe Thr Lys Glu Lys Asp Ile Val Ser Ala 
145                 150                 155                 160 
Val Val Lys Ala Thr Asn Gly Gly Ala His Gly Ile Ile Asn Val Ser 
                165                 170                 175     
Val Ser Glu Ala Ala Ile Glu Ala Ser Thr Arg Tyr Cys Arg Ala Asn 
            180                 185                 190         
Gly Thr Val Val Leu Val Gly Leu Pro Ala Gly Ala Lys Cys Ser Ser 
        195                 200                 205             
Asp Val Phe Asn His Val Val Lys Ser Ile Ser Ile Val Gly Ser Tyr 
    210                 215                 220                 
Val Gly Asn Arg Ala Asp Thr Arg Glu Ala Leu Asp Phe Phe Ala Arg 
225                 230                 235                 240 
Gly Leu Val Lys Ser Pro Ile Lys Val Val Gly Leu Ser Ser Leu Pro 
                245                 250                 255     
Glu Ile Tyr Glu Lys Met Glu Lys Gly Gln Ile Ala Gly Arg Tyr Val 
            260                 265                 270         
Val Asp Thr Ser Lys 
        275         

<210> 61
<211> 375
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> ADH3

<400> 61
Met Leu Arg Thr Ser Thr Leu Phe Thr Arg Arg Val Gln Pro Ser Leu 
1               5                   10                  15      
Phe Ser Arg Asn Ile Leu Arg Leu Gln Ser Thr Ala Ala Ile Pro Lys 
            20                  25                  30          
Thr Gln Lys Gly Val Ile Phe Tyr Glu Asn Lys Gly Lys Leu His Tyr 
        35                  40                  45              
Lys Asp Ile Pro Val Pro Glu Pro Lys Pro Asn Glu Ile Leu Ile Asn 
    50                  55                  60                  
Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His Ala Trp His Gly 
65                  70                  75                  80  
Asp Trp Pro Leu Pro Val Lys Leu Pro Leu Val Gly Gly His Glu Gly 
                85                  90                  95      
Ala Gly Val Val Val Lys Leu Gly Ser Asn Val Lys Gly Trp Lys Val 
            100                 105                 110         
Gly Asp Leu Ala Gly Ile Lys Trp Leu Asn Gly Ser Cys Met Thr Cys 
        115                 120                 125             
Glu Phe Cys Glu Ser Gly His Glu Ser Asn Cys Pro Asp Ala Asp Leu 
    130                 135                 140                 
Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln Phe Ala Thr Ala Asp 
145                 150                 155                 160 
Ala Ile Gln Ala Ala Lys Ile Gln Gln Gly Thr Asp Leu Ala Glu Val 
                165                 170                 175     
Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys Ala Leu Lys Glu 
            180                 185                 190         
Ala Asp Leu Lys Ala Gly Asp Trp Val Ala Ile Ser Gly Ala Ala Gly 
        195                 200                 205             
Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Thr Ala Met Gly Tyr Arg 
    210                 215                 220                 
Val Leu Gly Ile Asp Ala Gly Glu Glu Lys Glu Lys Leu Phe Lys Lys 
225                 230                 235                 240 
Leu Gly Gly Glu Val Phe Ile Asp Phe Thr Lys Thr Lys Asn Met Val 
                245                 250                 255     
Ser Asp Ile Gln Glu Ala Thr Lys Gly Gly Pro His Gly Val Ile Asn 
            260                 265                 270         
Val Ser Val Ser Glu Ala Ala Ile Ser Leu Ser Thr Glu Tyr Val Arg 
        275                 280                 285             
Pro Cys Gly Thr Val Val Leu Val Gly Leu Pro Ala Asn Ala Tyr Val 
    290                 295                 300                 
Lys Ser Glu Val Phe Ser His Val Val Lys Ser Ile Asn Ile Lys Gly 
305                 310                 315                 320 
Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu Ala Leu Asp Phe Phe 
                325                 330                 335     
Ser Arg Gly Leu Ile Lys Ser Pro Ile Lys Ile Val Gly Leu Ser Glu 
            340                 345                 350         
Leu Pro Lys Val Tyr Asp Leu Met Glu Lys Gly Lys Ile Leu Gly Arg 
        355                 360                 365             
Tyr Val Val Asp Thr Ser Lys 
    370                 375 

<210> 62
<211> 382
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> ADH4

<400> 62
Met Ser Ser Val Thr Gly Phe Tyr Ile Pro Pro Ile Ser Phe Phe Gly 
1               5                   10                  15      
Glu Gly Ala Leu Glu Glu Thr Ala Asp Tyr Ile Lys Asn Lys Asp Tyr 
            20                  25                  30          
Lys Lys Ala Leu Ile Val Thr Asp Pro Gly Ile Ala Ala Ile Gly Leu 
        35                  40                  45              
Ser Gly Arg Val Gln Lys Met Leu Glu Glu Arg Gly Leu Asn Val Ala 
    50                  55                  60                  
Ile Tyr Asp Lys Thr Gln Pro Asn Pro Asn Ile Ala Asn Val Thr Ala 
65                  70                  75                  80  
Gly Leu Lys Val Leu Lys Glu Glu Asn Ser Glu Ile Val Val Ser Ile 
                85                  90                  95      
Gly Gly Gly Ser Ala His Asp Asn Ala Lys Ala Ile Ala Leu Leu Ala 
            100                 105                 110         
Thr Asn Gly Gly Glu Ile Gly Asp Tyr Glu Gly Val Asn Gln Ser Lys 
        115                 120                 125             
Lys Ala Ala Leu Pro Leu Phe Ala Ile Asn Thr Thr Ala Gly Thr Ala 
    130                 135                 140                 
Ser Glu Met Thr Arg Phe Thr Ile Ile Ser Asn Glu Glu Lys Lys Ile 
145                 150                 155                 160 
Lys Met Ala Ile Ile Asp Asn Asn Val Thr Pro Ala Val Ala Val Asn 
                165                 170                 175     
Asp Pro Ser Thr Met Phe Gly Leu Pro Pro Ala Leu Thr Ala Ala Thr 
            180                 185                 190         
Gly Leu Asp Ala Leu Thr His Cys Ile Glu Ala Tyr Val Ser Thr Ala 
        195                 200                 205             
Ser Asn Pro Ile Thr Asp Ala Cys Ala Leu Lys Gly Ile Asp Leu Ile 
    210                 215                 220                 
Asn Glu Ser Leu Val Ala Ala Tyr Lys Asp Gly Lys Asp Lys Lys Ala 
225                 230                 235                 240 
Arg Thr Asp Met Cys Tyr Ala Glu Tyr Leu Ala Gly Met Ala Phe Asn 
                245                 250                 255     
Asn Ala Ser Leu Gly Tyr Val His Ala Leu Ala His Gln Leu Gly Gly 
            260                 265                 270         
Phe Tyr His Leu Pro His Gly Val Cys Asn Ala Val Leu Leu Pro His 
        275                 280                 285             
Val Gln Glu Ala Asn Met Gln Cys Pro Lys Ala Lys Lys Arg Leu Gly 
    290                 295                 300                 
Glu Ile Ala Leu His Cys Gly Ala Ser Gln Glu Asp Pro Glu Glu Thr 
305                 310                 315                 320 
Ile Lys Ala Leu His Val Leu Asn Arg Thr Met Asn Ile Pro Arg Asn 
                325                 330                 335     
Leu Lys Asp Leu Gly Val Lys Thr Glu Asp Phe Asp Ile Leu Ala Glu 
            340                 345                 350         
His Ala Met His Asp Ala Cys His Leu Thr Asn Pro Val Gln Phe Thr 
        355                 360                 365             
Lys Glu Gln Val Val Ala Ile Ile Lys Lys Ala Tyr Glu Tyr 
    370                 375                 380         

<210> 63
<211> 351
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> ADH5

<400> 63
Met Pro Ser Gln Val Ile Pro Glu Lys Gln Lys Ala Ile Val Phe Tyr 
1               5                   10                  15      
Glu Thr Asp Gly Lys Leu Glu Tyr Lys Asp Val Thr Val Pro Glu Pro 
            20                  25                  30          
Lys Pro Asn Glu Ile Leu Val His Val Lys Tyr Ser Gly Val Cys His 
        35                  40                  45              
Ser Asp Leu His Ala Trp His Gly Asp Trp Pro Phe Gln Leu Lys Phe 
    50                  55                  60                  
Pro Leu Ile Gly Gly His Glu Gly Ala Gly Val Val Val Lys Leu Gly 
65                  70                  75                  80  
Ser Asn Val Lys Gly Trp Lys Val Gly Asp Phe Ala Gly Ile Lys Trp 
                85                  90                  95      
Leu Asn Gly Thr Cys Met Ser Cys Glu Tyr Cys Glu Val Gly Asn Glu 
            100                 105                 110         
Ser Gln Cys Pro Tyr Leu Asp Gly Thr Gly Phe Thr His Asp Gly Thr 
        115                 120                 125             
Phe Gln Glu Tyr Ala Thr Ala Asp Ala Val Gln Ala Ala His Ile Pro 
    130                 135                 140                 
Pro Asn Val Asn Leu Ala Glu Val Ala Pro Ile Leu Cys Ala Gly Ile 
145                 150                 155                 160 
Thr Val Tyr Lys Ala Leu Lys Arg Ala Asn Val Ile Pro Gly Gln Trp 
                165                 170                 175     
Val Thr Ile Ser Gly Ala Cys Gly Gly Leu Gly Ser Leu Ala Ile Gln 
            180                 185                 190         
Tyr Ala Leu Ala Met Gly Tyr Arg Val Ile Gly Ile Asp Gly Gly Asn 
        195                 200                 205             
Ala Lys Arg Lys Leu Phe Glu Gln Leu Gly Gly Glu Ile Phe Ile Asp 
    210                 215                 220                 
Phe Thr Glu Glu Lys Asp Ile Val Gly Ala Ile Ile Lys Ala Thr Asn 
225                 230                 235                 240 
Gly Gly Ser His Gly Val Ile Asn Val Ser Val Ser Glu Ala Ala Ile 
                245                 250                 255     
Glu Ala Ser Thr Arg Tyr Cys Arg Pro Asn Gly Thr Val Val Leu Val 
            260                 265                 270         
Gly Met Pro Ala His Ala Tyr Cys Asn Ser Asp Val Phe Asn Gln Val 
        275                 280                 285             
Val Lys Ser Ile Ser Ile Val Gly Ser Cys Val Gly Asn Arg Ala Asp 
    290                 295                 300                 
Thr Arg Glu Ala Leu Asp Phe Phe Ala Arg Gly Leu Ile Lys Ser Pro 
305                 310                 315                 320 
Ile His Leu Ala Gly Leu Ser Asp Val Pro Glu Ile Phe Ala Lys Met 
                325                 330                 335     
Glu Lys Gly Glu Ile Val Gly Arg Tyr Val Val Glu Thr Ser Lys 
            340                 345                 350     

<210> 64
<211> 360
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> ADH6

<400> 64
Met Ser Tyr Pro Glu Lys Phe Glu Gly Ile Ala Ile Gln Ser His Glu 
1               5                   10                  15      
Asp Trp Lys Asn Pro Lys Lys Thr Lys Tyr Asp Pro Lys Pro Phe Tyr 
            20                  25                  30          
Asp His Asp Ile Asp Ile Lys Ile Glu Ala Cys Gly Val Cys Gly Ser 
        35                  40                  45              
Asp Ile His Cys Ala Ala Gly His Trp Gly Asn Met Lys Met Pro Leu 
    50                  55                  60                  
Val Val Gly His Glu Ile Val Gly Lys Val Val Lys Leu Gly Pro Lys 
65                  70                  75                  80  
Ser Asn Ser Gly Leu Lys Val Gly Gln Arg Val Gly Val Gly Ala Gln 
                85                  90                  95      
Val Phe Ser Cys Leu Glu Cys Asp Arg Cys Lys Asn Asp Asn Glu Pro 
            100                 105                 110         
Tyr Cys Thr Lys Phe Val Thr Thr Tyr Ser Gln Pro Tyr Glu Asp Gly 
        115                 120                 125             
Tyr Val Ser Gln Gly Gly Tyr Ala Asn Tyr Val Arg Val His Glu His 
    130                 135                 140                 
Phe Val Val Pro Ile Pro Glu Asn Ile Pro Ser His Leu Ala Ala Pro 
145                 150                 155                 160 
Leu Leu Cys Gly Gly Leu Thr Val Tyr Ser Pro Leu Val Arg Asn Gly 
                165                 170                 175     
Cys Gly Pro Gly Lys Lys Val Gly Ile Val Gly Leu Gly Gly Ile Gly 
            180                 185                 190         
Ser Met Gly Thr Leu Ile Ser Lys Ala Met Gly Ala Glu Thr Tyr Val 
        195                 200                 205             
Ile Ser Arg Ser Ser Arg Lys Arg Glu Asp Ala Met Lys Met Gly Ala 
    210                 215                 220                 
Asp His Tyr Ile Ala Thr Leu Glu Glu Gly Asp Trp Gly Glu Lys Tyr 
225                 230                 235                 240 
Phe Asp Thr Phe Asp Leu Ile Val Val Cys Ala Ser Ser Leu Thr Asp 
                245                 250                 255     
Ile Asp Phe Asn Ile Met Pro Lys Ala Met Lys Val Gly Gly Arg Ile 
            260                 265                 270         
Val Ser Ile Ser Ile Pro Glu Gln His Glu Met Leu Ser Leu Lys Pro 
        275                 280                 285             
Tyr Gly Leu Lys Ala Val Ser Ile Ser Tyr Ser Ala Leu Gly Ser Ile 
    290                 295                 300                 
Lys Glu Leu Asn Gln Leu Leu Lys Leu Val Ser Glu Lys Asp Ile Lys 
305                 310                 315                 320 
Ile Trp Val Glu Thr Leu Pro Val Gly Glu Ala Gly Val His Glu Ala 
                325                 330                 335     
Phe Glu Arg Met Glu Lys Gly Asp Val Arg Tyr Arg Phe Thr Leu Val 
            340                 345                 350         
Gly Tyr Asp Lys Glu Phe Ser Asp 
        355                 360 

<210> 65
<211> 206
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> ALD2

<400> 65
Met Ala Cys Trp Lys Leu Gln Gly Ala Leu Ala Ala Gly Asn Thr Val 
1               5                   10                  15      
Ile Ile Lys Pro Ala Glu Asn Thr Ser Leu Ser Leu Leu Tyr Phe Ala 
            20                  25                  30          
Thr Leu Ile Lys Lys Ala Gly Phe Pro Pro Gly Val Val Asn Ile Val 
        35                  40                  45              
Pro Gly Tyr Gly Ser Leu Val Gly Gln Ala Leu Ala Ser His Met Asp 
    50                  55                  60                  
Ile Asp Lys Ile Ser Phe Thr Gly Ser Thr Lys Val Gly Gly Phe Val 
65                  70                  75                  80  
Leu Glu Ala Ser Gly Gln Ser Asn Leu Lys Asp Val Thr Leu Glu Cys 
                85                  90                  95      
Gly Gly Lys Ser Pro Ala Leu Val Asp Glu Ile Phe Gly Pro Val Val 
            100                 105                 110         
Val Val Ser Lys Phe Thr Asn Tyr Asp Asp Ala Leu Lys Leu Ala Asn 
        115                 120                 125             
Asp Thr Cys Tyr Gly Leu Ala Ser Ala Val Phe Thr Lys Asp Val Lys 
    130                 135                 140                 
Lys Ala His Met Phe Ala Arg Asp Ile Lys Ala Gly Thr Val Trp Ile 
145                 150                 155                 160 
Asn Ser Ser Asn Asp Glu Asp Val Thr Val Pro Phe Gly Gly Phe Lys 
                165                 170                 175     
Met Ser Gly Ile Gly Arg Glu Leu Gly Gln Ser Gly Val Asp Thr Tyr 
            180                 185                 190         
Leu Gln Thr Lys Ala Val His Ile Asn Leu Ser Leu Asp Asn 
        195                 200                 205     

<210> 66
<211> 263
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> ALD3

<400> 66
Met Gly Glu Thr Ile Pro Leu Thr Phe Asn Lys Phe Ala Tyr Thr Leu 
1               5                   10                  15      
Lys Val Pro Phe Gly Val Val Ala Gln Ile Val Pro Trp Asn Tyr Pro 
            20                  25                  30          
Leu Ala Met Ala Cys Arg Lys Met Gln Gly Ala Leu Ala Ala Gly Asn 
        35                  40                  45              
Thr Val Ile Ile Lys Pro Ala Glu Asn Thr Ser Leu Ser Leu Leu Tyr 
    50                  55                  60                  
Phe Ala Thr Leu Ile Lys Lys Ala Gly Phe Pro Pro Gly Val Val Asn 
65                  70                  75                  80  
Val Ile Pro Gly Tyr Gly Ser Val Val Gly Lys Ala Leu Gly Thr His 
                85                  90                  95      
Met Asp Ile Asp Lys Ile Ser Phe Thr Gly Ser Thr Lys Val Gly Gly 
            100                 105                 110         
Ser Val Leu Glu Ala Ser Gly Gln Ser Asn Leu Lys Asp Ile Thr Leu 
        115                 120                 125             
Glu Cys Gly Gly Lys Ser Pro Ala Leu Val Phe Glu Asp Ala Asp Leu 
    130                 135                 140                 
Asp Lys Ala Ile Glu Trp Val Ala Asn Gly Lys Thr Ser Lys Leu Leu 
145                 150                 155                 160 
Arg Asp Glu Ile Phe Gly Pro Val Val Val Val Ser Lys Phe Thr Asn 
                165                 170                 175     
Tyr Asp Asp Ala Leu Lys Leu Ala Asn Asp Thr Cys Tyr Gly Leu Ala 
            180                 185                 190         
Ser Ala Val Phe Thr Lys Asp Val Lys Lys Ala His Met Phe Ala Arg 
        195                 200                 205             
Asp Ile Lys Ala Gly Thr Val Trp Ile Asn Gln Thr Asn Gln Glu Glu 
    210                 215                 220                 
Ala Lys Val Pro Phe Gly Gly Phe Lys Met Ser Gly Ile Gly Arg Glu 
225                 230                 235                 240 
Ser Gly Asp Thr Gly Val Asp Asn Tyr Leu Gln Ile Lys Ser Val His 
                245                 250                 255     
Val Asp Leu Ser Leu Asp Lys 
            260             

<210> 67
<211> 519
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> ALD4

<400> 67
Met Phe Ser Arg Ser Thr Leu Cys Leu Lys Thr Ser Ala Ser Ser Ile 
1               5                   10                  15      
Gly Arg Leu Gln Leu Arg Tyr Phe Ser His Leu Pro Met Thr Val Pro 
            20                  25                  30          
Ile Lys Leu Pro Asn Gly Leu Glu Tyr Glu Gln Pro Thr Gly Leu Phe 
        35                  40                  45              
Ile Asn Asn Lys Phe Val Pro Ser Lys Gln Asn Lys Thr Phe Glu Val 
    50                  55                  60                  
Ile Asn Pro Ser Thr Glu Glu Glu Ile Cys His Ile Tyr Glu Gly Arg 
65                  70                  75                  80  
Glu Asp Asp Val Glu Glu Ala Val Gln Ala Ala Asp Arg Ala Phe Ser 
                85                  90                  95      
Asn Gly Ser Trp Asn Gly Ile Asp Pro Ile Asp Arg Gly Lys Ala Leu 
            100                 105                 110         
Tyr Arg Leu Ala Glu Leu Ile Glu Gln Asp Lys Asp Val Ile Ala Ser 
        115                 120                 125             
Ile Glu Thr Leu Asp Asn Gly Lys Ala Ile Ser Ser Ser Arg Gly Asp 
    130                 135                 140                 
Val Asp Leu Val Ile Asn Tyr Leu Lys Ser Ser Ala Gly Phe Ala Asp 
145                 150                 155                 160 
Lys Ile Asp Gly Arg Met Ile Asp Thr Gly Arg Thr His Phe Ser Tyr 
                165                 170                 175     
Thr Lys Arg Gln Pro Leu Gly Val Cys Gly Gln Ile Ile Pro Trp Asn 
            180                 185                 190         
Phe Pro Leu Leu Met Trp Ala Trp Lys Ile Ala Pro Ala Leu Val Thr 
        195                 200                 205             
Gly Asn Thr Val Val Leu Lys Thr Ala Glu Ser Thr Pro Leu Ser Ala 
    210                 215                 220                 
Leu Tyr Val Ser Lys Tyr Ile Pro Gln Ala Gly Ile Pro Pro Gly Val 
225                 230                 235                 240 
Ile Asn Ile Val Ser Gly Phe Gly Lys Ile Val Gly Glu Ala Ile Thr 
                245                 250                 255     
Asn His Pro Lys Ile Lys Lys Val Ala Phe Thr Gly Ser Thr Ala Thr 
            260                 265                 270         
Gly Arg His Ile Tyr Gln Ser Ala Ala Ala Gly Leu Lys Lys Val Thr 
        275                 280                 285             
Leu Glu Leu Gly Gly Lys Ser Pro Asn Ile Val Phe Ala Asp Ala Glu 
    290                 295                 300                 
Leu Lys Lys Ala Val Gln Asn Ile Ile Leu Gly Ile Tyr Tyr Asn Ser 
305                 310                 315                 320 
Gly Glu Val Cys Cys Ala Gly Ser Arg Val Tyr Val Glu Glu Ser Ile 
                325                 330                 335     
Tyr Asp Lys Phe Ile Glu Glu Phe Lys Ala Ala Ser Glu Ser Ile Lys 
            340                 345                 350         
Val Gly Asp Pro Phe Asp Glu Ser Thr Phe Gln Gly Ala Gln Thr Ser 
        355                 360                 365             
Gln Met Gln Leu Asn Lys Ile Leu Lys Tyr Val Asp Ile Gly Lys Asn 
    370                 375                 380                 
Glu Gly Ala Thr Leu Ile Thr Gly Gly Glu Arg Leu Gly Ser Lys Gly 
385                 390                 395                 400 
Tyr Phe Ile Lys Pro Thr Val Phe Gly Asp Val Lys Glu Asp Met Arg 
                405                 410                 415     
Ile Val Lys Glu Glu Ile Phe Gly Pro Val Val Thr Val Thr Lys Phe 
            420                 425                 430         
Lys Ser Ala Asp Glu Val Ile Asn Met Ala Asn Asp Ser Glu Tyr Gly 
        435                 440                 445             
Leu Ala Ala Gly Ile His Thr Ser Asn Ile Asn Thr Ala Leu Lys Val 
    450                 455                 460                 
Ala Asp Arg Val Asn Ala Gly Thr Val Trp Ile Asn Thr Tyr Asn Asp 
465                 470                 475                 480 
Phe His His Ala Val Pro Phe Gly Gly Phe Asn Ala Ser Gly Leu Gly 
                485                 490                 495     
Arg Glu Met Ser Val Asp Ala Leu Gln Asn Tyr Leu Gln Val Lys Ala 
            500                 505                 510         
Val Arg Ala Lys Leu Asp Glu 
        515                 

<210> 68
<211> 520
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> ALD5

<400> 68
Met Leu Ser Arg Thr Arg Ala Ala Ala Pro Asn Ser Arg Ile Phe Thr 
1               5                   10                  15      
Arg Ser Leu Leu Arg Leu Tyr Ser Gln Ala Pro Leu Arg Val Pro Ile 
            20                  25                  30          
Thr Leu Pro Asn Gly Phe Thr Tyr Glu Gln Pro Thr Gly Leu Phe Ile 
        35                  40                  45              
Asn Gly Glu Phe Val Ala Ser Lys Gln Lys Lys Thr Phe Asp Val Ile 
    50                  55                  60                  
Asn Pro Ser Asn Glu Glu Lys Ile Thr Thr Val Tyr Lys Ala Met Glu 
65                  70                  75                  80  
Asp Asp Val Asp Glu Ala Val Ala Ala Ala Lys Lys Ala Phe Glu Thr 
                85                  90                  95      
Lys Trp Ser Ile Val Glu Pro Glu Val Arg Ala Lys Ala Leu Phe Asn 
            100                 105                 110         
Leu Ala Asp Leu Val Glu Lys His Gln Glu Thr Leu Ala Ala Ile Glu 
        115                 120                 125             
Ser Met Asp Asn Gly Lys Ser Leu Phe Cys Ala Arg Gly Asp Val Ala 
    130                 135                 140                 
Leu Val Ser Lys Tyr Leu Arg Ser Cys Gly Gly Trp Ala Asp Lys Ile 
145                 150                 155                 160 
Tyr Gly Asn Val Ile Asp Thr Gly Lys Asn His Phe Thr Tyr Ser Ile 
                165                 170                 175     
Lys Glu Pro Leu Gly Val Cys Gly Gln Ile Ile Pro Trp Asn Phe Pro 
            180                 185                 190         
Leu Leu Met Trp Ser Trp Lys Ile Gly Pro Ala Leu Ala Thr Gly Asn 
        195                 200                 205             
Thr Val Val Leu Lys Pro Ala Glu Thr Thr Pro Leu Ser Ala Leu Phe 
    210                 215                 220                 
Ala Ser Gln Leu Cys Gln Glu Ala Gly Ile Pro Ala Gly Val Val Asn 
225                 230                 235                 240 
Ile Leu Pro Gly Ser Gly Arg Val Val Gly Glu Arg Leu Ser Ala His 
                245                 250                 255     
Pro Asp Val Lys Lys Ile Ala Phe Thr Gly Ser Thr Ala Thr Gly Arg 
            260                 265                 270         
His Ile Met Lys Val Ala Ala Asp Thr Val Lys Lys Val Thr Leu Glu 
        275                 280                 285             
Leu Gly Gly Lys Ser Pro Asn Ile Val Phe Ala Asp Ala Asp Leu Asp 
    290                 295                 300                 
Lys Ala Val Lys Asn Ile Ala Phe Gly Ile Phe Tyr Asn Ser Gly Glu 
305                 310                 315                 320 
Val Cys Cys Ala Gly Ser Arg Ile Tyr Ile Gln Asp Thr Val Tyr Glu 
                325                 330                 335     
Glu Val Leu Gln Lys Leu Lys Asp Tyr Thr Glu Ser Leu Lys Val Gly 
            340                 345                 350         
Asp Pro Phe Asp Glu Glu Val Phe Gln Gly Ala Gln Thr Ser Asp Lys 
        355                 360                 365             
Gln Leu His Lys Ile Leu Asp Tyr Val Asp Val Ala Lys Ser Glu Gly 
    370                 375                 380                 
Ala Arg Leu Val Thr Gly Gly Ala Arg His Gly Ser Lys Gly Tyr Phe 
385                 390                 395                 400 
Val Lys Pro Thr Val Phe Ala Asp Val Lys Glu Asp Met Arg Ile Val 
                405                 410                 415     
Lys Glu Glu Val Phe Gly Pro Ile Val Thr Val Ser Lys Phe Ser Thr 
            420                 425                 430         
Val Asp Glu Val Ile Ala Met Ala Asn Asp Ser Gln Tyr Gly Leu Ala 
        435                 440                 445             
Ala Gly Ile His Thr Asn Asp Ile Asn Lys Ala Val Asp Val Ser Lys 
    450                 455                 460                 
Arg Val Lys Ala Gly Thr Val Trp Ile Asn Thr Tyr Asn Asn Phe His 
465                 470                 475                 480 
Gln Asn Val Pro Phe Gly Gly Phe Gly Gln Ser Gly Ile Gly Arg Glu 
                485                 490                 495     
Met Gly Glu Ala Ala Leu Ser Asn Tyr Thr Gln Thr Lys Ser Val Arg 
            500                 505                 510         
Ile Ala Ile Asp Lys Pro Ile Arg 
        515                 520 

<210> 69
<211> 500
<212> PRT
<213> Saccharomyces cerevisiae

<220> 
<223> ALD6

<400> 69
Met Thr Lys Leu His Phe Asp Thr Ala Glu Pro Val Lys Ile Thr Leu 
1               5                   10                  15      
Pro Asn Gly Leu Thr Tyr Glu Gln Pro Thr Gly Leu Phe Ile Asn Asn 
            20                  25                  30          
Lys Phe Met Lys Ala Gln Asp Gly Lys Thr Tyr Pro Val Glu Asp Pro 
        35                  40                  45              
Ser Thr Glu Asn Thr Val Cys Glu Val Ser Ser Ala Thr Thr Glu Asp 
    50                  55                  60                  
Val Glu Tyr Ala Ile Glu Cys Ala Asp Arg Ala Phe His Asp Thr Glu 
65                  70                  75                  80  
Trp Ala Thr Gln Asp Pro Arg Glu Arg Gly Arg Leu Leu Ser Lys Leu 
                85                  90                  95      
Ala Asp Glu Leu Glu Ser Gln Ile Asp Leu Val Ser Ser Ile Glu Ala 
            100                 105                 110         
Leu Asp Asn Gly Lys Thr Leu Ala Leu Ala Arg Gly Asp Val Thr Ile 
        115                 120                 125             
Ala Ile Asn Cys Leu Arg Asp Ala Ala Ala Tyr Ala Asp Lys Val Asn 
    130                 135                 140                 
Gly Arg Thr Ile Asn Thr Gly Asp Gly Tyr Met Asn Phe Thr Thr Leu 
145                 150                 155                 160 
Glu Pro Ile Gly Val Cys Gly Gln Ile Ile Pro Trp Asn Phe Pro Ile 
                165                 170                 175     
Met Met Leu Ala Trp Lys Ile Ala Pro Ala Leu Ala Met Gly Asn Val 
            180                 185                 190         
Cys Ile Leu Lys Pro Ala Ala Val Thr Pro Leu Asn Ala Leu Tyr Phe 
        195                 200                 205             
Ala Ser Leu Cys Lys Lys Val Gly Ile Pro Ala Gly Val Val Asn Ile 
    210                 215                 220                 
Val Pro Gly Pro Gly Arg Thr Val Gly Ala Ala Leu Thr Asn Asp Pro 
225                 230                 235                 240 
Arg Ile Arg Lys Leu Ala Phe Thr Gly Ser Thr Glu Val Gly Lys Ser 
                245                 250                 255     
Val Ala Val Asp Ser Ser Glu Ser Asn Leu Lys Lys Ile Thr Leu Glu 
            260                 265                 270         
Leu Gly Gly Lys Ser Ala His Leu Val Phe Asp Asp Ala Asn Ile Lys 
        275                 280                 285             
Lys Thr Leu Pro Asn Leu Val Asn Gly Ile Phe Lys Asn Ala Gly Gln 
    290                 295                 300                 
Ile Cys Ser Ser Gly Ser Arg Ile Tyr Val Gln Glu Gly Ile Tyr Asp 
305                 310                 315                 320 
Glu Leu Leu Ala Ala Phe Lys Ala Tyr Leu Glu Thr Glu Ile Lys Val 
                325                 330                 335     
Gly Asn Pro Phe Asp Lys Ala Asn Phe Gln Gly Ala Ile Thr Asn Arg 
            340                 345                 350         
Gln Gln Phe Asp Thr Ile Met Asn Tyr Ile Asp Ile Gly Lys Lys Glu 
        355                 360                 365             
Gly Ala Lys Ile Leu Thr Gly Gly Glu Lys Val Gly Asp Lys Gly Tyr 
    370                 375                 380                 
Phe Ile Arg Pro Thr Val Phe Tyr Asp Val Asn Glu Asp Met Arg Ile 
385                 390                 395                 400 
Val Lys Glu Glu Ile Phe Gly Pro Val Val Thr Val Ala Lys Phe Lys 
                405                 410                 415     
Thr Leu Glu Glu Gly Val Glu Met Ala Asn Ser Ser Glu Phe Gly Leu 
            420                 425                 430         
Gly Ser Gly Ile Glu Thr Glu Ser Leu Ser Thr Gly Leu Lys Val Ala 
        435                 440                 445             
Lys Met Leu Lys Ala Gly Thr Val Trp Ile Asn Thr Tyr Asn Asp Phe 
    450                 455                 460                 
Asp Ser Arg Val Pro Phe Gly Gly Val Lys Gln Ser Gly Tyr Gly Arg 
465                 470                 475                 480 
Glu Met Gly Glu Glu Val Tyr His Ala Tyr Thr Glu Val Lys Ala Val 
                485                 490                 495     
Arg Ile Lys Leu 
            500 

<210> 70
<211> 499
<212> PRT
<213> Arabidopsis thaliana

<220> 
<223> fumarase

<400> 70
Met Ala Ala Leu Thr Met Gln Phe Glu Gly Glu Lys Lys Asn Val Ser 
1               5                   10                  15      
Glu Val Ala Asp Val Thr Leu Lys Gln Glu Asp Glu Gln Gln Glu Arg 
            20                  25                  30          
Arg Ser Tyr Ser Thr Pro Phe Arg Glu Glu Arg Asp Thr Phe Gly Pro 
        35                  40                  45              
Ile Gln Val Pro Ser Asp Lys Leu Trp Gly Ala Gln Thr Gln Arg Ser 
    50                  55                  60                  
Leu Gln Asn Phe Glu Ile Gly Gly Asp Arg Glu Arg Met Pro Glu Pro 
65                  70                  75                  80  
Ile Val Arg Ala Phe Gly Val Leu Lys Lys Cys Ala Ala Lys Val Asn 
                85                  90                  95      
Met Glu Tyr Gly Leu Asp Pro Met Ile Gly Glu Ala Ile Met Glu Ala 
            100                 105                 110         
Ala Gln Glu Val Ala Glu Gly Lys Leu Asn Asp His Phe Pro Leu Val 
        115                 120                 125             
Val Trp Gln Thr Gly Ser Gly Thr Gln Ser Asn Met Asn Ala Asn Glu 
    130                 135                 140                 
Val Ile Ala Asn Arg Ala Ala Glu Ile Leu Gly His Lys Arg Gly Glu 
145                 150                 155                 160 
Lys Ile Val His Pro Asn Asp His Val Asn Arg Ser Gln Ser Ser Asn 
                165                 170                 175     
Asp Thr Phe Pro Thr Val Met His Ile Ala Ala Ala Thr Glu Ile Thr 
            180                 185                 190         
Ser Arg Leu Ile Pro Ser Leu Lys Asn Leu His Ser Ser Leu Glu Ser 
        195                 200                 205             
Lys Ser Phe Glu Phe Lys Asp Ile Val Lys Ile Gly Arg Thr His Thr 
    210                 215                 220                 
Gln Asp Ala Thr Pro Leu Thr Leu Gly Gln Glu Phe Gly Gly Tyr Ala 
225                 230                 235                 240 
Thr Gln Val Glu Tyr Gly Leu Asn Arg Val Ala Cys Thr Leu Pro Arg 
                245                 250                 255     
Ile Tyr Gln Leu Ala Gln Gly Gly Thr Ala Val Gly Thr Gly Leu Asn 
            260                 265                 270         
Thr Lys Lys Gly Phe Asp Val Lys Ile Ala Ala Ala Val Ala Glu Glu 
        275                 280                 285             
Thr Asn Leu Pro Phe Val Thr Ala Glu Asn Lys Phe Glu Ala Leu Ala 
    290                 295                 300                 
Ala His Asp Ala Cys Val Glu Thr Ser Gly Ser Leu Asn Thr Ile Ala 
305                 310                 315                 320 
Thr Ser Leu Met Lys Ile Ala Asn Asp Ile Arg Phe Leu Gly Ser Gly 
                325                 330                 335     
Pro Arg Cys Gly Leu Gly Glu Leu Ser Leu Pro Glu Asn Glu Pro Gly 
            340                 345                 350         
Ser Ser Ile Met Pro Gly Lys Val Asn Pro Thr Gln Cys Glu Ala Leu 
        355                 360                 365             
Thr Met Val Cys Ala Gln Val Met Gly Asn His Val Ala Val Thr Ile 
    370                 375                 380                 
Gly Gly Ser Asn Gly His Phe Glu Leu Asn Val Phe Lys Pro Val Ile 
385                 390                 395                 400 
Ala Ser Ala Leu Leu His Ser Ile Arg Leu Ile Ala Asp Ala Ser Ala 
                405                 410                 415     
Ser Phe Glu Lys Asn Cys Val Arg Gly Ile Glu Ala Asn Arg Glu Arg 
            420                 425                 430         
Ile Ser Lys Leu Leu His Glu Ser Leu Met Leu Val Thr Ser Leu Asn 
        435                 440                 445             
Pro Lys Ile Gly Tyr Asp Asn Ala Ala Ala Val Ala Lys Arg Ala His 
    450                 455                 460                 
Lys Glu Gly Cys Thr Leu Lys His Ala Ala Met Lys Leu Gly Val Leu 
465                 470                 475                 480 
Thr Ser Glu Glu Phe Asp Thr Leu Val Val Pro Glu Lys Met Ile Gly 
                485                 490                 495     
Pro Ser Asp 
            

