                         SEQUENCE LISTING

<110>  The Regents of the University of California
 
<120>  TE2FT ENZYME FOR ENZYMATIC SYNTHESIS OF ALPHA 1-2-FUCOSIDES

<130>  076916-1033647-215510PC

<150>  US 62/269,574
<151>  2015-12-18

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  293
<212>  PRT
<213>  Thermosynechococcus elongatus

<400>  1

Met Ile Ile Val His Leu Cys Gly Gly Leu Gly Asn Gln Met Phe Gln 
1               5                   10                  15      


Tyr Ala Ala Gly Leu Ala Ala Ala His Arg Ile Gly Ser Glu Val Lys 
            20                  25                  30          


Phe Asp Thr His Trp Phe Asp Ala Thr Cys Leu His Gln Gly Leu Glu 
        35                  40                  45              


Leu Arg Arg Val Phe Gly Leu Glu Leu Pro Glu Pro Ser Ser Lys Asp 
    50                  55                  60                  


Leu Arg Lys Val Leu Gly Ala Cys Val His Pro Ala Val Arg Arg Leu 
65                  70                  75                  80  


Leu Ala Gly His Phe Leu His Gly Leu Arg Pro Lys Ser Leu Val Ile 
                85                  90                  95      


Gln Pro His Phe His Tyr Trp Thr Gly Phe Glu His Leu Pro Asp Asn 
            100                 105                 110         


Val Tyr Leu Glu Gly Tyr Trp Gln Ser Glu Arg Tyr Phe Ser Asn Ile 
        115                 120                 125             


Ala Asp Ile Ile Arg Gln Gln Phe Arg Phe Val Glu Pro Leu Asp Pro 
    130                 135                 140                 


His Asn Ala Ala Leu Met Asp Glu Met Gln Ser Gly Val Ser Val Ser 
145                 150                 155                 160 


Leu His Ile Arg Arg Gly Asp Tyr Phe Asn Asn Pro Gln Met Arg Arg 
                165                 170                 175     


Val His Gly Val Asp Leu Ser Glu Tyr Tyr Pro Ala Ala Val Ala Thr 
            180                 185                 190         


Met Ile Glu Lys Thr Asn Ala Glu Arg Phe Tyr Val Phe Ser Asp Asp 
        195                 200                 205             


Pro Gln Trp Val Leu Glu His Leu Lys Leu Pro Val Ser Tyr Thr Val 
    210                 215                 220                 


Val Asp His Asn Arg Gly Ala Ala Ser Tyr Arg Asp Met Gln Leu Met 
225                 230                 235                 240 


Ser Ala Cys Arg His His Ile Ile Ala Asn Ser Thr Phe Ser Trp Trp 
                245                 250                 255     


Gly Ala Trp Leu Asn Pro Arg Pro Asp Lys Val Val Ile Ala Pro Arg 
            260                 265                 270         


His Trp Phe Asn Val Asp Val Phe Asp Thr Arg Asp Leu Tyr Cys Pro 
        275                 280                 285             


Gly Trp Ile Val Leu 
    290             


<210>  2
<211>  969
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  2

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Gln Lys Leu Leu Ser Leu Pro Pro Asn Leu Val 
            20                  25                  30          


Gln Ser Phe His Glu Leu Glu Arg Val Asn Arg Thr Asp Trp Phe Tyr 
        35                  40                  45              


Thr Ser Asp Pro Val Gly Lys Lys Leu Gly Ser Gly Gly Gly Thr Ser 
    50                  55                  60                  


Trp Leu Leu Glu Glu Cys Tyr Asn Glu Tyr Ser Asp Gly Ala Thr Phe 
65                  70                  75                  80  


Gly Glu Trp Leu Glu Lys Glu Lys Arg Ile Leu Leu His Ala Gly Gly 
                85                  90                  95      


Gln Ser Arg Arg Leu Pro Gly Tyr Ala Pro Ser Gly Lys Ile Leu Thr 
            100                 105                 110         


Pro Val Pro Val Phe Arg Trp Glu Arg Gly Gln His Leu Gly Gln Asn 
        115                 120                 125             


Leu Leu Ser Leu Gln Leu Pro Leu Tyr Glu Lys Ile Met Ser Leu Ala 
    130                 135                 140                 


Pro Asp Lys Leu His Thr Leu Ile Ala Ser Gly Asp Val Tyr Ile Arg 
145                 150                 155                 160 


Ser Glu Lys Pro Leu Gln Ser Ile Pro Glu Ala Asp Val Val Cys Tyr 
                165                 170                 175     


Gly Leu Trp Val Asp Pro Ser Leu Ala Thr His His Gly Val Phe Ala 
            180                 185                 190         


Ser Asp Arg Lys His Pro Glu Gln Leu Asp Phe Met Leu Gln Lys Pro 
        195                 200                 205             


Ser Leu Ala Glu Leu Glu Ser Leu Ser Lys Thr His Leu Phe Leu Met 
    210                 215                 220                 


Asp Ile Gly Ile Trp Leu Leu Ser Asp Arg Ala Val Glu Ile Leu Met 
225                 230                 235                 240 


Lys Arg Ser His Lys Glu Ser Ser Glu Glu Leu Lys Tyr Tyr Asp Leu 
                245                 250                 255     


Tyr Ser Asp Phe Gly Leu Ala Leu Gly Thr His Pro Arg Ile Glu Asp 
            260                 265                 270         


Glu Glu Val Asn Thr Leu Ser Val Ala Ile Leu Pro Leu Pro Gly Gly 
        275                 280                 285             


Glu Phe Tyr His Tyr Gly Thr Ser Lys Glu Leu Ile Ser Ser Thr Leu 
    290                 295                 300                 


Ser Val Gln Asn Lys Val Tyr Asp Gln Arg Arg Ile Met His Arg Lys 
305                 310                 315                 320 


Val Lys Pro Asn Pro Ala Met Phe Val Gln Asn Ala Val Val Arg Ile 
                325                 330                 335     


Pro Leu Cys Ala Glu Asn Ala Asp Leu Trp Ile Glu Asn Ser His Ile 
            340                 345                 350         


Gly Pro Lys Trp Lys Ile Ala Ser Arg His Ile Ile Thr Gly Val Pro 
        355                 360                 365             


Glu Asn Asp Trp Ser Leu Ala Val Pro Ala Gly Val Cys Val Asp Val 
    370                 375                 380                 


Val Pro Met Gly Asp Lys Gly Phe Val Ala Arg Pro Tyr Gly Leu Asp 
385                 390                 395                 400 


Asp Val Phe Lys Gly Asp Leu Arg Asp Ser Lys Thr Thr Leu Thr Gly 
                405                 410                 415     


Ile Pro Phe Gly Glu Trp Met Ser Lys Arg Gly Leu Ser Tyr Thr Asp 
            420                 425                 430         


Leu Lys Gly Arg Thr Asp Asp Leu Gln Ala Val Ser Val Phe Pro Met 
        435                 440                 445             


Val Asn Ser Val Glu Glu Leu Gly Leu Val Leu Arg Trp Met Leu Ser 
    450                 455                 460                 


Glu Pro Glu Leu Glu Glu Gly Lys Asn Ile Trp Leu Arg Ser Glu His 
465                 470                 475                 480 


Phe Ser Ala Asp Glu Ile Ser Ala Gly Ala Asn Leu Lys Arg Leu Tyr 
                485                 490                 495     


Ala Gln Arg Glu Glu Phe Arg Lys Gly Asn Trp Gln Ala Leu Ala Val 
            500                 505                 510         


Asn His Glu Lys Ser Val Phe Tyr Gln Leu Asp Leu Ala Asp Ala Ala 
        515                 520                 525             


Glu Asp Phe Val Arg Leu Gly Leu Asp Met Pro Glu Leu Leu Pro Glu 
    530                 535                 540                 


Asp Ala Leu Gln Met Ser Arg Ile His Asn Arg Met Leu Arg Ala Arg 
545                 550                 555                 560 


Ile Leu Lys Leu Asp Gly Lys Asp Tyr Arg Pro Glu Glu Gln Ala Ala 
                565                 570                 575     


Phe Asp Leu Leu Arg Asp Gly Leu Leu Asp Gly Ile Ser Asn Arg Lys 
            580                 585                 590         


Ser Thr Pro Lys Leu Asp Val Tyr Ser Asp Gln Ile Val Trp Gly Arg 
        595                 600                 605             


Ser Pro Val Arg Ile Asp Met Ala Gly Gly Trp Thr Asp Thr Pro Pro 
    610                 615                 620                 


Tyr Ser Leu Tyr Ser Gly Gly Asn Val Val Asn Leu Ala Ile Glu Leu 
625                 630                 635                 640 


Asn Gly Gln Pro Pro Leu Gln Val Tyr Val Lys Pro Cys Lys Asp Phe 
                645                 650                 655     


His Ile Val Leu Arg Ser Ile Asp Met Gly Ala Met Glu Ile Val Ser 
            660                 665                 670         


Thr Phe Asp Glu Leu Gln Asp Tyr Lys Lys Ile Gly Ser Pro Phe Ser 
        675                 680                 685             


Ile Pro Lys Ala Ala Leu Ser Leu Ala Gly Phe Ala Pro Ala Phe Ser 
    690                 695                 700                 


Ala Val Ser Tyr Ala Ser Leu Glu Glu Gln Leu Lys Asp Phe Gly Ala 
705                 710                 715                 720 


Gly Ile Glu Val Thr Leu Leu Ala Ala Ile Pro Ala Gly Ser Gly Leu 
                725                 730                 735     


Gly Thr Ser Ser Ile Leu Ala Ser Thr Val Leu Gly Ala Ile Asn Asp 
            740                 745                 750         


Phe Cys Gly Leu Ala Trp Asp Lys Asn Glu Ile Cys Gln Arg Thr Leu 
        755                 760                 765             


Val Leu Glu Gln Leu Leu Thr Thr Gly Gly Gly Trp Gln Asp Gln Tyr 
    770                 775                 780                 


Gly Gly Val Leu Gln Gly Val Lys Leu Leu Gln Thr Glu Ala Gly Phe 
785                 790                 795                 800 


Ala Gln Ser Pro Leu Val Arg Trp Leu Pro Asp His Leu Phe Thr His 
                805                 810                 815     


Pro Glu Tyr Lys Asp Cys His Leu Leu Tyr Tyr Thr Gly Ile Thr Arg 
            820                 825                 830         


Thr Ala Lys Gly Ile Leu Ala Glu Ile Val Ser Ser Met Phe Leu Asn 
        835                 840                 845             


Ser Ser Leu His Leu Asn Leu Leu Ser Glu Met Lys Ala His Ala Leu 
    850                 855                 860                 


Asp Met Asn Glu Ala Ile Gln Arg Gly Ser Phe Val Glu Phe Gly Arg 
865                 870                 875                 880 


Leu Val Gly Lys Thr Trp Glu Gln Asn Lys Ala Leu Asp Ser Gly Thr 
                885                 890                 895     


Asn Pro Pro Ala Val Glu Ala Ile Ile Asp Leu Ile Lys Asp Tyr Thr 
            900                 905                 910         


Leu Gly Tyr Lys Leu Pro Gly Ala Gly Gly Gly Gly Tyr Leu Tyr Met 
        915                 920                 925             


Val Ala Lys Asp Pro Gln Ala Ala Val Arg Ile Arg Lys Ile Leu Thr 
    930                 935                 940                 


Glu Asn Ala Pro Asn Pro Arg Ala Arg Phe Val Glu Met Thr Leu Ser 
945                 950                 955                 960 


Asp Lys Gly Phe Gln Val Ser Arg Ser 
                965                 


<210>  3
<211>  183
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  3

Met Gly Leu Glu Thr Val Pro Ala Gly Lys Ala Leu Pro Asp Asp Ile 
1               5                   10                  15      


Tyr Val Val Ile Glu Ile Pro Ala Asn Ser Asp Pro Ile Lys Tyr Glu 
            20                  25                  30          


Val Asp Lys Glu Ser Gly Ala Leu Phe Val Asp Arg Phe Met Ala Thr 
        35                  40                  45              


Ala Met Phe Tyr Pro Ala Asn Tyr Gly Tyr Val Asn Asn Thr Leu Ser 
    50                  55                  60                  


Leu Asp Gly Asp Pro Val Asp Val Leu Val Pro Thr Pro Tyr Pro Leu 
65                  70                  75                  80  


Gln Pro Gly Ser Val Ile Arg Cys Arg Pro Val Gly Val Leu Lys Met 
                85                  90                  95      


Thr Asp Glu Ala Gly Ser Asp Ala Lys Val Val Ala Val Pro His Ser 
            100                 105                 110         


Lys Leu Thr Lys Glu Tyr Asp His Ile Lys Asp Val Asn Asp Leu Pro 
        115                 120                 125             


Ala Leu Leu Lys Ala Gln Ile Gln His Phe Phe Glu Ser Tyr Lys Ala 
    130                 135                 140                 


Leu Glu Ala Gly Lys Trp Val Lys Val Asp Gly Trp Glu Gly Val Asp 
145                 150                 155                 160 


Ala Ala Arg Gln Glu Ile Leu Asp Ser Phe Glu Arg Ala Lys Lys Leu 
                165                 170                 175     


Glu His His His His His His 
            180             


<210>  4
<211>  2910
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  4
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atgcaaaaac tactatcttt accgtccaat ctggttcagt cttttcatga actggagagg      120

gtgaatcgta ccgattggtt ttgtacttcc gacccggtag gtaagaaact tggttccggt      180

ggtggaacat cctggctgct tgaagaatgt tataatgaat attcagatgg tgctactttt      240

ggagagtggc ttgaaaaaga aaaaagaatt cttcttcatg cgggtgggca aagccgtcgt      300

ttacccggct atgcaccttc tggaaagatt ctcactccgg ttcctgtgtt ccggtgggag      360

agagggcaac atctgggaca aaatctgctt tctctgcaac ttcccctata tgaaaaaatc      420

atgtctttgg ctccggataa actccataca ctgattgcga gtggtgatgt ctatattcgt      480

tcggagaaac ctttgcagag tattcccgaa gcggatgtgg tttgttatgg actgtgggta      540

gatccgtctc tggctaccca tcatggcgtg tttgcttccg atcgcaaaca tcccgaacaa      600

ctcgacttta tgcttcagaa gccttcgttg gcagaattgg aatctttatc gaagacccat      660

ttgttcctga tggacatcgg tatatggctt ttgagtgacc gtgccgtaga aatcttgatg      720

aaacgttctc ataaagaaag ctctgaagaa ctaaagtatt atgatcttta ttccgatttt      780

ggattagctt tgggaactca tccccgtatt gaagacgaag aggtcaatac gctatccgtt      840

gctattctgc ctttgccggg aggagagttc tatcattacg ggaccagtaa agaactgatt      900

tcttcaactc tttccgtaca gaataaggtt tacgatcagc gtcgtatcat gcaccgtaaa      960

gtaaagccca atccggctat gtttgtccaa aatgctgtcg tgcggatacc tctttgtgcc     1020

gagaatgctg atttatggat cgagaacagt catatcggac caaagtggaa gattgcttca     1080

cgacatatta ttaccggggt tccggaaaat gactggtcat tggctgtgcc tgccggagtg     1140

tgtgtagatg tggttccgat gggtgataag ggctttgttg cccgtccata cggtctggac     1200

gatgttttca aaggagattt gagagattcc aaaacaaccc tgacgggtat tccttttggt     1260

gaatggatgt ccaaacgcgg tttgtcatat acagatttga aaggacgtac ggacgattta     1320

caggcagttt ccgtattccc tatggttaat tctgtagaag agttgggatt ggtgttgagg     1380

tggatgttgt ccgaacccga actggaggaa ggaaagaata tctggttacg ttccgaacat     1440

ttttctgcgg acgaaatttc ggcaggtgcc aatctgaagc gtttgtatgc acaacgtgaa     1500

gagttcagaa aaggaaactg gaaagcattg gccgttaatc atgaaaaaag tgttttttat     1560

caacttgatt tggccgatgc agctgaagat tttgtacgtc ttggtttgga tatgcctgaa     1620

ttattgcctg aggatgctct gcagatgtca cgcatccata accggatgtt gcgtgcgcgt     1680

attttgaaat tagacgggaa agattatcgt ccggaagaac aggctgcttt tgatttgctt     1740

cgtgacggct tgctggacgg gatcagtaat cgtaagagta ccccaaaatt ggatgtatat     1800

tccgatcaga ttgtttgggg acgtagcccc gtgcgcatcg atatggcagg tggatggacc     1860

gatactcctc cttattcact ttattcggga ggaaatgtgg tgaatctagc cattgagttg     1920

aacggacaac ctcccttaca ggtctatgtg aagccgtgta aagacttcca tatcgtcctg     1980

cgttctatcg atatgggtgc tatggaaata gtatctacgt ttgatgaatt gcaagattat     2040

aagaagatcg gttcaccttt ctctattccg aaagccgctc tgtcattggc aggctttgca     2100

cctgcgtttt ctgctgtatc ttatgcttca ttagaggaac agcttaaaga tttcggtgca     2160

ggtattgaag tgactttatt ggctgctatt cctgccggtt ccggtttggg caccagttcc     2220

attctggctt ctaccgtact tggtgccatt aacgatttct gtggtttagc ctgggataaa     2280

aatgagattt gtcaacgtac tcttgttctt gaacaattgc tgactaccgg aggtggatgg     2340

caggatcagt atggaggtgt gttgcagggt gtgaagcttc ttcagaccga ggccggcttt     2400

gctcaaagtc cattggtgcg ttggctaccc gatcatttat ttacgcatcc tgaatacaaa     2460

gactgtcact tgctttatta taccggtata actcgtacgg caaaagggat cttggcagaa     2520

atagtcagtt ccatgttcct caattcatcg ttgcatctca atttactttc ggaaatgaag     2580

gcgcatgcat tggatatgaa tgaagctata cagcgtggaa gttttgttga gtttggccgt     2640

ttggtaggaa aaacctggga acaaaacaaa gcattggata gcggaacaaa tcctccggct     2700

gtggaggcaa ttatcgatct gataaaagat tataccttgg gatataaatt gccgggagcc     2760

ggtggtggcg ggtacttata tatggtagcg aaagatccgc aagctgctgt tcgtattcgt     2820

aagatactga cagaaaacgc tccgaatccg cgggcacgtt ttgtcgaaat gacgttatct     2880

gataagggat tccaagtatc acgatcataa                                      2910


<210>  5
<211>  552
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  5
atgggtttag aaaccgtacc ggcaggtaaa gcattgccag atgatattta tgttgtgatt       60

gaaattcctg ctaattcaga tccaattaaa tatgaagtgg ataaagaaag tggcgcgttg      120

tttgttgacc gttttatggc gacagcgatg ttctatccag cgaactatgg ttatgtgaat      180

aacacattgt ctttagatgg tgaccccgtt gatgtattag tcccgacacc atacccatta      240

caaccgggct cagtgattcg ttgccgtcca gttggggtat taaaaatgac agatgaagct      300

ggaagtgatg cgaaagtggt ggcagtacca cacagcaaat taaccaaaga atacgatcat      360

attaaagatg tgaatgattt accggcatta ttaaaagcac aaatccaaca cttcttcgaa      420

agctacaaag cattagaagc gggtaaatgg gtgaaagtag atggttggga aggtgttgat      480

gccgcccgtc aagaaatttt agattcattt gaacgtgcaa aaaaactcga gcaccaccac      540

caccaccact ga                                                          552


<210>  6
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  6
gatccatatg attatcgttc acctgtgcg                                         29


<210>  7
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  7
aagggatcct tacagaacaa tccaaccc                                          28


<210>  8
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  8
gatccatatg gtgaaagtac tgactgtatt                                        30


<210>  9
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  9
acgcgtcgac ttagtggtgg tggtggtggt gcagaacaat ccaaccc                     47


