                    
<110>  LIST
       Sergeant Kjell
 
<120>  Method for enzymatically modifying the tri-dimensional structure of a protein

<130>  sequence listing of PT04271 WO

<160>  6   

<210>  1
<211>  22
<212>  PRT
<213>  Alfalfa contig 53863 (part of)	

<223>  Beta subunit of polygalacturonase

<400>  1

Ser Asp Phe Ser Gly Tyr Ser Glu Lys Gly Ser Ser Ala Val Asp Ala Phe Val Ser Tyr 
798             802                 807                 812                 817
Ser Lys  
818 



<210>  2
<211>  644
<212>  PRT
<213>  Alfalfa contig Medtr8g064530

<223>  Beta subunit of polygalacturonase

<400>  2
 
Met Lys Lys Met Gln Lys Gln Leu Gly His Ile Ile His Thr Leu Phe Ile Leu Leu Ser
1               5                   10                  15		    20 	  
Ile Leu Thr Val Gly Leu Ala Ile Asn His Glu Lys Glu Lys Asn Gln Asn Pro Phe Thr
21              25                  30			35		    40	
Pro Lys Ala Tyr Val Ile Arg Tyr Trp Asp Arg Val Ile Lys Asn Asn Leu Pro Lys Pro
41		45		    50			55		    60	
Ser Phe Ile Leu Ser Lys Ala Ser Pro Leu Ser Ala Thr Glu Ala Ala Thr Phe Thr Lys
61		65		    70			75		    80
His Ala Ala Ala Asn Thr Leu Ser Thr Lys Leu Pro Glu Phe Cys Ser Ala Ala His Leu
81		85		    90			95		    100
Phe Cys Phe Pro Glu Leu Thr Lys Gln Arg Pro Phe Asp Pro Ser His Phe Thr Val Tyr
101		105		    110			115		    120	
Glu Thr Asp Gln Asn Phe Thr Phe Tyr Ser Leu Gly Glu Asn Phe Thr Thr Tyr Gly Thr
121		125		    130			135		    140
His Lys Leu Asn Gly Ile Asp Ala Phe Lys Asn Tyr Ser Asn Gly Leu Tyr Asn Glu Lys
141		145		    150			155		    160 	  	
His Asn Glu Phe Ala Arg Tyr Ser Lys Ile Ser Thr Gly His Asn Asp Ser Phe Lys Asn
161		165		    170			175		    180
Tyr Ala Leu Asn Asn Asn Glu Gly Val Gln Thr Phe Asn Ser Tyr Gly Thr Lys Ser Met
181		185		    190			195		    200
Ala Gly Phe Gly Glu Phe Lys Ala Tyr Ser Glu Glu Ser Asn Ile Pro Asn Leu Lys Phe
201		205		    210			215		    220
Thr Thr Tyr Thr Ala Ser Val Thr Gly Arg Glu Gln Ser Phe Val Ser Tyr Ser Asp Gly
221		225		    230			235		    240		
Gly Asn Ser Gly Asn Gln Ser Phe Ala Ser Tyr Gly Lys Asp Ser Ile His Ala Val Asn
241		245		    250			255		    260	
Gly Phe Thr Gln Tyr Ala Lys Asn Ala Asn Val Phe Lys Ser Asp Phe Ser Gly Tyr Ser
261      	265		    270			275		    280	
Glu Lys Gly Ser Ser Ala Val Asp Ala Phe Val Ser Tyr Ser Lys Asn Leu Asn Lys Pro
281		285		    290			295		    300 
Glu Asn Thr Phe Lys Asn Tyr Gly Lys Gly Ser Phe Ala Ala Gln Glu Lys Phe Thr Asn
301		305		    310			315		    320
Tyr Arg Asp Gly Ala Gly Ser Gly Asp Asp Ser Phe Thr Ser Tyr Gly Glu Asn Ser Gln
321		325		    330			335		    340	
Arg Gly Val Lys Val Asp Phe Asn Asn Tyr Gly Gln Asn Pro Asp Tyr Pro Lys Ser Pro
341		345		    350			355		    360	
Gly Ser Glu Thr Phe Lys Gly Tyr Ala Lys Gly Ala Asp Leu Asp His Lys Val Ser Phe
361		365		    370			375		    380
Lys Val Tyr Asn Gly Asn Asn Asn Thr Phe Lys Asp Tyr Asp Lys Lys Gly Ile Ser Phe
381		385		    390			395		    400		
Ala Ser Tyr Asn Lys Thr Thr Thr Ser Thr Asn Ser Leu Ser Asp Ser Gly Ser Leu Ala
401		405		    410			415		    420
Lys Asn Met Val Gln Pro Gly Lys Phe Phe Arg Glu Lys Met Leu Lys Glu Gly Thr Val
421		425		    430			435		    440	
Met Pro Met Pro Asp Ile Arg Asp Lys Leu Pro Pro Arg Ser Phe Leu Pro Arg Ser Ile
441		445		    450			455		    460	
Leu Thr Lys Leu Pro Phe Ala Ser Ser Lys Leu Asn Glu Met Lys Gln Val Phe Lys Val
461		465		    470			475		    480	
Ser Glu Asn Ser Ser Met Asn Lys Met Met Val Asp Ala Leu Ser Glu Cys Glu Arg Ala
481		485		    490			495		    500  	
Pro Ser Val Gly Glu Thr Lys Arg Cys Val Gly Ser Leu Glu Asp Met Ile Asp Phe Ala
501		505		    510			515		    520
Thr Ser Val Leu Gly His Ser Val Thr Val Arg Ser Thr Glu Asn Val Asn Gly Ala Gly
521		525		    530			535		    540	
Lys Asp Val Met Val Gly Lys Val Lys Gly Ile Asn Gly Gly Lys Val Thr Glu Ser Val
541		545		    550			555		    560 			
Ser Cys His Gln Ser Leu Phe Pro Tyr Leu Leu Tyr Tyr Cys His Ser Val Pro Asn Val
561		565		    570			575		    580	
His Val Tyr Glu Ala Asp Leu Leu Asp Pro Val Ser Lys Ala Lys Ile Asn His Gly Val
581		585		    590			595		    600	
Ala Ile Cys His Leu Asp Thr Thr Ala Trp Ser Ser Thr His Gly Ala Phe Met Ala Leu
601		605		    610			615		    620
Gly Ser Ala Pro Gly Gln Ile Glu Val Cys His Trp Ile Phe Glu Asn Asp Met Thr Trp
621		625		    630			635		    640		
Ser Ser Ala Asp
641

<210>  3
<211>  30
<212>  PRT
<213>  Alfalfa contig Medtr8g064530 (part of)

<223>  Focus sequence

<400>  3

Gln Thr Phe Asn Ser Tyr Gly Thr Lys Ser Met Ala Gly Phe Gly Glu Phe Lys Ala Tyr
190             194                 199                 204		    209 	  
Ser Glu Glu Ser Asn Ile Pro Asn Leu Lys
210             214                 219	

<210>  4
<211>  42
<212>  PRT
<213>  Alfalfa contig 53863 (part of)	

<223>  Beta subunit of polygalacturonase 

<400>  4	

Asp Thr Asn Val Pro Asn Leu Arg Phe Thr Thr Tyr Ser Val Gly Val Ala Gly Arg Gln 
94              98                  103			108		    113
Gln Thr Phe Thr Ser Tyr Ser Glu Ala Gly Asn Ala Gly Asp Gln Ser Phe Gly Asn Tyr 
114             118                 123			128		    133
Gly Lys
134

<210>  5
<211>  57
<212>  PRT
<213>  Alfalfa contig 53863 (part of)	

<223>  Beta subunit of polygalacturonase

<400>  5

Gly Ser Leu Gly Ala Val Glu Lys Phe Ser Asn Tyr Arg Asp Gln Ala Asn Val Gly Ala 
192             196                 201                 206		    211 
Asp Ser Phe Ser Ser Tyr Ala Lys Asp Ser Thr Gly Gly Thr His Val Asp Phe Asp Asn 
212             216                 221 		226		    231
Tyr Gly Lys Ser Phe Asn Glu Gly Thr Asp Ser Phe Lys Gly Tyr Ala Lys
232		236		    241			246	

<210>  6
<211>  10
<212>  PRT
<213>  Alfalfa contig 53863 (part of)	

<223>  Beta subunit of polygalacturonase

<400>  6

Lys Gly Ile Ser Phe Ala Gly Tyr Thr Lys
273             277                 282 



