                         SEQUENCE LISTING

<110>  Alexion Pharmaceuticals, Inc.
       Lasaro, Melissa
       Cobaugh, Christian
       Vasaveda, Haren
       Osterman, David
 
<120>  Modified mRNA Encoding a Uridine Diphosphate Glucuronosyl 
       Transferase and Uses Thereof

<130>  0380

<150>  62/265,174
<151>  2015-12-09

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  47
<212>  RNA
<213>  Homo sapiens

<400>  1
gggaaauaag agagaaaaga agaguaagaa gaaauauaag agccacc                     47


<210>  2
<211>  1599
<212>  DNA
<213>  Homo sapiens

<400>  2
atggctgtgg agtcccaggg cggacgccca cttgtcctgg gcctgctgct gtgtgtgctg       60

ggcccagtgg tgtcccatgc tgggaagata ctgttgatcc cagtggatgg cagccactgg      120

ctgagcatgc ttggggccat ccagcagctg cagcagaggg gacatgaaat agttgtccta      180

gcacctgacg cctcgttgta catcagagac ggagcatttt acaccttgaa gacgtaccct      240

gtgccattcc aaagggagga tgtgaaagag tcttttgtta gtctcgggca taatgttttt      300

gagaatgatt ctttcctgca gcgtgtgatc aaaacataca agaaaataaa aaaggactct      360

gctatgcttt tgtctggctg ttcccactta ctgcacaaca aggagctcat ggcctccctg      420

gcagaaagca gctttgatgt catgctgacg gaccctttcc ttccttgcag ccccatcgtg      480

gcccagtacc tgtctctgcc cactgtattc ttcttgcatg cactgccatg cagcctggaa      540

tttgaggcta cccagtgccc caacccattc tcctacgtgc ccaggcctct ctcctctcat      600

tcagatcaca tgaccttcct gcagcgggtg aagaacatgc tcattgcctt ttcacagaac      660

tttctgtgcg acgtggttta ttccccgtat gcaacccttg cctcagaatt ccttcagaga      720

gaggtgactg tccaggacct attgagctct gcatctgtct ggctgtttag aagtgacttt      780

gtgaaggatt accctaggcc catcatgccc aatatggttt ttgttggtgg aatcaactgc      840

cttcaccaaa atccactatc ccaggaattt gaagcctaca ttaatgcttc tggagaacat      900

ggaattgtgg ttttctcttt gggatcaatg gtctcagaaa ttccagagaa gaaagctatg      960

gcaattgctg atgctttggg caaaatccct cagacagtcc tgtggcggta cactggaacc     1020

cgaccatcga atcttgcgaa caacacgata cttgttaagt ggctacccca aaacgatctg     1080

cttggtcacc cgatgacccg tgcctttatc acccatgctg gttcccatgg tgtttatgaa     1140

agcatatgca atggcgttcc catggtgatg atgcccttgt ttggtgatca gatggacaat     1200

gcaaagcgca tggagactaa gggagctgga gtgaccctga atgttctgga aatgacttct     1260

gaagatttag aaaatgctct aaaagcagtc atcaatgaca aaagttacaa ggagaacatc     1320

atgcgcctct ccagccttca caaggaccgc ccggtggagc cgctggacct ggccgtgttc     1380

tgggtggagt ttgtgatgag gcacaagggc gcgccacacc tgcgccccgc agcccacgac     1440

ctcacctggt accagtacca ttccttggac gtgattggtt tcctcttggc cgtcgtgctg     1500

acagtggcct tcatcacctt taaatgttgt gcttatggct accggaaatg cttggggaaa     1560

aaagggcgag ttaagaaagc ccacaaatcc aagacccat                            1599


<210>  3
<211>  119
<212>  RNA
<213>  Homo sapiens

<400>  3
ugauaauagg cuggagccuc gguggccaug cuucuugccc cuugggccuc cccccagccc       60

cuccuccccu uccugcaccc guacccccgu ggucuuugaa uaaagucuga gugggcggc       119


<210>  4
<211>  533
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Ala Val Glu Ser Gln Gly Gly Arg Pro Leu Val Leu Gly Leu Leu 
1               5                   10                  15      


Leu Cys Val Leu Gly Pro Val Val Ser His Ala Gly Lys Ile Leu Leu 
            20                  25                  30          


Ile Pro Val Asp Gly Ser His Trp Leu Ser Met Leu Gly Ala Ile Gln 
        35                  40                  45              


Gln Leu Gln Gln Arg Gly His Glu Ile Val Val Leu Ala Pro Asp Ala 
    50                  55                  60                  


Ser Leu Tyr Ile Arg Asp Gly Ala Phe Tyr Thr Leu Lys Thr Tyr Pro 
65                  70                  75                  80  


Val Pro Phe Gln Arg Glu Asp Val Lys Glu Ser Phe Val Ser Leu Gly 
                85                  90                  95      


His Asn Val Phe Glu Asn Asp Ser Phe Leu Gln Arg Val Ile Lys Thr 
            100                 105                 110         


Tyr Lys Lys Ile Lys Lys Asp Ser Ala Met Leu Leu Ser Gly Cys Ser 
        115                 120                 125             


His Leu Leu His Asn Lys Glu Leu Met Ala Ser Leu Ala Glu Ser Ser 
    130                 135                 140                 


Phe Asp Val Met Leu Thr Asp Pro Phe Leu Pro Cys Ser Pro Ile Val 
145                 150                 155                 160 


Ala Gln Tyr Leu Ser Leu Pro Thr Val Phe Phe Leu His Ala Leu Pro 
                165                 170                 175     


Cys Ser Leu Glu Phe Glu Ala Thr Gln Cys Pro Asn Pro Phe Ser Tyr 
            180                 185                 190         


Val Pro Arg Pro Leu Ser Ser His Ser Asp His Met Thr Phe Leu Gln 
        195                 200                 205             


Arg Val Lys Asn Met Leu Ile Ala Phe Ser Gln Asn Phe Leu Cys Asp 
    210                 215                 220                 


Val Val Tyr Ser Pro Tyr Ala Thr Leu Ala Ser Glu Phe Leu Gln Arg 
225                 230                 235                 240 


Glu Val Thr Val Gln Asp Leu Leu Ser Ser Ala Ser Val Trp Leu Phe 
                245                 250                 255     


Arg Ser Asp Phe Val Lys Asp Tyr Pro Arg Pro Ile Met Pro Asn Met 
            260                 265                 270         


Val Phe Val Gly Gly Ile Asn Cys Leu His Gln Asn Pro Leu Ser Gln 
        275                 280                 285             


Glu Phe Glu Ala Tyr Ile Asn Ala Ser Gly Glu His Gly Ile Val Val 
    290                 295                 300                 


Phe Ser Leu Gly Ser Met Val Ser Glu Ile Pro Glu Lys Lys Ala Met 
305                 310                 315                 320 


Ala Ile Ala Asp Ala Leu Gly Lys Ile Pro Gln Thr Val Leu Trp Arg 
                325                 330                 335     


Tyr Thr Gly Thr Arg Pro Ser Asn Leu Ala Asn Asn Thr Ile Leu Val 
            340                 345                 350         


Lys Trp Leu Pro Gln Asn Asp Leu Leu Gly His Pro Met Thr Arg Ala 
        355                 360                 365             


Phe Ile Thr His Ala Gly Ser His Gly Val Tyr Glu Ser Ile Cys Asn 
    370                 375                 380                 


Gly Val Pro Met Val Met Met Pro Leu Phe Gly Asp Gln Met Asp Asn 
385                 390                 395                 400 


Ala Lys Arg Met Glu Thr Lys Gly Ala Gly Val Thr Leu Asn Val Leu 
                405                 410                 415     


Glu Met Thr Ser Glu Asp Leu Glu Asn Ala Leu Lys Ala Val Ile Asn 
            420                 425                 430         


Asp Lys Ser Tyr Lys Glu Asn Ile Met Arg Leu Ser Ser Leu His Lys 
        435                 440                 445             


Asp Arg Pro Val Glu Pro Leu Asp Leu Ala Val Phe Trp Val Glu Phe 
    450                 455                 460                 


Val Met Arg His Lys Gly Ala Pro His Leu Arg Pro Ala Ala His Asp 
465                 470                 475                 480 


Leu Thr Trp Tyr Gln Tyr His Ser Leu Asp Val Ile Gly Phe Leu Leu 
                485                 490                 495     


Ala Val Val Leu Thr Val Ala Phe Ile Thr Phe Lys Cys Cys Ala Tyr 
            500                 505                 510         


Gly Tyr Arg Lys Cys Leu Gly Lys Lys Gly Arg Val Lys Lys Ala His 
        515                 520                 525             


Lys Ser Lys Thr His 
    530             


<210>  5
<211>  1623
<212>  DNA
<213>  artificial

<220>
<223>  human UGT1A1 with a C-terminal FLAG tag

<400>  5
atggctgtgg agtcccaggg cggacgccca cttgtcctgg gcctgctgct gtgtgtgctg       60

ggcccagtgg tgtcccatgc tgggaagata ctgttgatcc cagtggatgg cagccactgg      120

ctgagcatgc ttggggccat ccagcagctg cagcagaggg gacatgaaat agttgtccta      180

gcacctgacg cctcgttgta catcagagac ggagcatttt acaccttgaa gacgtaccct      240

gtgccattcc aaagggagga tgtgaaagag tcttttgtta gtctcgggca taatgttttt      300

gagaatgatt ctttcctgca gcgtgtgatc aaaacataca agaaaataaa aaaggactct      360

gctatgcttt tgtctggctg ttcccactta ctgcacaaca aggagctcat ggcctccctg      420

gcagaaagca gctttgatgt catgctgacg gaccctttcc ttccttgcag ccccatcgtg      480

gcccagtacc tgtctctgcc cactgtattc ttcttgcatg cactgccatg cagcctggaa      540

tttgaggcta cccagtgccc caacccattc tcctacgtgc ccaggcctct ctcctctcat      600

tcagatcaca tgaccttcct gcagcgggtg aagaacatgc tcattgcctt ttcacagaac      660

tttctgtgcg acgtggttta ttccccgtat gcaacccttg cctcagaatt ccttcagaga      720

gaggtgactg tccaggacct attgagctct gcatctgtct ggctgtttag aagtgacttt      780

gtgaaggatt accctaggcc catcatgccc aatatggttt ttgttggtgg aatcaactgc      840

cttcaccaaa atccactatc ccaggaattt gaagcctaca ttaatgcttc tggagaacat      900

ggaattgtgg ttttctcttt gggatcaatg gtctcagaaa ttccagagaa gaaagctatg      960

gcaattgctg atgctttggg caaaatccct cagacagtcc tgtggcggta cactggaacc     1020

cgaccatcga atcttgcgaa caacacgata cttgttaagt ggctacccca aaacgatctg     1080

cttggtcacc cgatgacccg tgcctttatc acccatgctg gttcccatgg tgtttatgaa     1140

agcatatgca atggcgttcc catggtgatg atgcccttgt ttggtgatca gatggacaat     1200

gcaaagcgca tggagactaa gggagctgga gtgaccctga atgttctgga aatgacttct     1260

gaagatttag aaaatgctct aaaagcagtc atcaatgaca aaagttacaa ggagaacatc     1320

atgcgcctct ccagccttca caaggaccgc ccggtggagc cgctggacct ggccgtgttc     1380

tgggtggagt ttgtgatgag gcacaagggc gcgccacacc tgcgccccgc agcccacgac     1440

ctcacctggt accagtacca ttccttggac gtgattggtt tcctcttggc cgtcgtgctg     1500

acagtggcct tcatcacctt taaatgttgt gcttatggct accggaaatg cttggggaaa     1560

aaagggcgag ttaagaaagc ccacaaatcc aagacccatg actacaaaga cgatgacgac     1620

aag                                                                   1623


<210>  6
<211>  541
<212>  PRT
<213>  artificial

<220>
<223>  human UGT1A1 with a C-terminal FLAG tag

<400>  6

Met Ala Val Glu Ser Gln Gly Gly Arg Pro Leu Val Leu Gly Leu Leu 
1               5                   10                  15      


Leu Cys Val Leu Gly Pro Val Val Ser His Ala Gly Lys Ile Leu Leu 
            20                  25                  30          


Ile Pro Val Asp Gly Ser His Trp Leu Ser Met Leu Gly Ala Ile Gln 
        35                  40                  45              


Gln Leu Gln Gln Arg Gly His Glu Ile Val Val Leu Ala Pro Asp Ala 
    50                  55                  60                  


Ser Leu Tyr Ile Arg Asp Gly Ala Phe Tyr Thr Leu Lys Thr Tyr Pro 
65                  70                  75                  80  


Val Pro Phe Gln Arg Glu Asp Val Lys Glu Ser Phe Val Ser Leu Gly 
                85                  90                  95      


His Asn Val Phe Glu Asn Asp Ser Phe Leu Gln Arg Val Ile Lys Thr 
            100                 105                 110         


Tyr Lys Lys Ile Lys Lys Asp Ser Ala Met Leu Leu Ser Gly Cys Ser 
        115                 120                 125             


His Leu Leu His Asn Lys Glu Leu Met Ala Ser Leu Ala Glu Ser Ser 
    130                 135                 140                 


Phe Asp Val Met Leu Thr Asp Pro Phe Leu Pro Cys Ser Pro Ile Val 
145                 150                 155                 160 


Ala Gln Tyr Leu Ser Leu Pro Thr Val Phe Phe Leu His Ala Leu Pro 
                165                 170                 175     


Cys Ser Leu Glu Phe Glu Ala Thr Gln Cys Pro Asn Pro Phe Ser Tyr 
            180                 185                 190         


Val Pro Arg Pro Leu Ser Ser His Ser Asp His Met Thr Phe Leu Gln 
        195                 200                 205             


Arg Val Lys Asn Met Leu Ile Ala Phe Ser Gln Asn Phe Leu Cys Asp 
    210                 215                 220                 


Val Val Tyr Ser Pro Tyr Ala Thr Leu Ala Ser Glu Phe Leu Gln Arg 
225                 230                 235                 240 


Glu Val Thr Val Gln Asp Leu Leu Ser Ser Ala Ser Val Trp Leu Phe 
                245                 250                 255     


Arg Ser Asp Phe Val Lys Asp Tyr Pro Arg Pro Ile Met Pro Asn Met 
            260                 265                 270         


Val Phe Val Gly Gly Ile Asn Cys Leu His Gln Asn Pro Leu Ser Gln 
        275                 280                 285             


Glu Phe Glu Ala Tyr Ile Asn Ala Ser Gly Glu His Gly Ile Val Val 
    290                 295                 300                 


Phe Ser Leu Gly Ser Met Val Ser Glu Ile Pro Glu Lys Lys Ala Met 
305                 310                 315                 320 


Ala Ile Ala Asp Ala Leu Gly Lys Ile Pro Gln Thr Val Leu Trp Arg 
                325                 330                 335     


Tyr Thr Gly Thr Arg Pro Ser Asn Leu Ala Asn Asn Thr Ile Leu Val 
            340                 345                 350         


Lys Trp Leu Pro Gln Asn Asp Leu Leu Gly His Pro Met Thr Arg Ala 
        355                 360                 365             


Phe Ile Thr His Ala Gly Ser His Gly Val Tyr Glu Ser Ile Cys Asn 
    370                 375                 380                 


Gly Val Pro Met Val Met Met Pro Leu Phe Gly Asp Gln Met Asp Asn 
385                 390                 395                 400 


Ala Lys Arg Met Glu Thr Lys Gly Ala Gly Val Thr Leu Asn Val Leu 
                405                 410                 415     


Glu Met Thr Ser Glu Asp Leu Glu Asn Ala Leu Lys Ala Val Ile Asn 
            420                 425                 430         


Asp Lys Ser Tyr Lys Glu Asn Ile Met Arg Leu Ser Ser Leu His Lys 
        435                 440                 445             


Asp Arg Pro Val Glu Pro Leu Asp Leu Ala Val Phe Trp Val Glu Phe 
    450                 455                 460                 


Val Met Arg His Lys Gly Ala Pro His Leu Arg Pro Ala Ala His Asp 
465                 470                 475                 480 


Leu Thr Trp Tyr Gln Tyr His Ser Leu Asp Val Ile Gly Phe Leu Leu 
                485                 490                 495     


Ala Val Val Leu Thr Val Ala Phe Ile Thr Phe Lys Cys Cys Ala Tyr 
            500                 505                 510         


Gly Tyr Arg Lys Cys Leu Gly Lys Lys Gly Arg Val Lys Lys Ala His 
        515                 520                 525             


Lys Ser Lys Thr His Asp Tyr Lys Asp Asp Asp Asp Lys 
    530                 535                 540     


<210>  7
<211>  1605
<212>  DNA
<213>  Rattus norvegicus

<400>  7
atgtccgtgg tgtgccggag ctcatgttcg cttctgcttc ttccgtgcct tctgctgtgt       60

gtgttgggtc cctctgcgtc ccatgctggg aagctgttag tgatccccat agatggcagc      120

cactggctga gtatgctcgg agttattcag cagctccagc aaaaggggca cgaagtggtg      180

gtcatagcac ctgaagcttc gatacacata aaagaaggat cattttacac tatgaggaag      240

taccctgtgc cattccaaaa tgaaaacgtg acagctgctt ttgtggaact tgggcggagt      300

gtctttgatc aagatccttt tctgctgcgt gtggttaaaa catacaacaa agtcaaaagg      360

gactccagta tgctgctgtc tggctgctcc caccttctgc acaatgccga gtttatggcc      420

tctctggaac aaagccactt tgatgctctg ctgacagacc ctttccttcc gtgtggctcc      480

attgtggccc agtacctgtc tctgcctgct gtgtacttct tgaatgcatt gccatgcagc      540

ctggatttgg aagccaccca atgccctgct ccgttgtcct acgtgcccaa gagtttgtcc      600

tcgaacacag atcgcatgaa cttcctgcag cgggtgaaga acatgattat tgctttgaca      660

gagaactttc tatgcagagt ggtttactcc ccctatgggt cacttgccac tgaaatctta      720

cagaaagagg tgactgtcaa ggaccttctg agtcctgcat ctatctggct gatgagaaac      780

gactttgtga aagattaccc caggcccatc atgcccaaca tggtttttat tggtgggata      840

aactgccttc agaaaaaagc cctatcccag gaatttgaag cctatgtcaa cgcctccgga      900

gaacatggca tcgtggtttt ctctttggga tccatggtct cagagattcc agagaagaaa      960

gcgatggaaa ttgctgaggc tttgggcaga attcctcaga cggtcctgtg gcgctacacc     1020

ggaactagac catcgaacct tgcaaagaac actattcttg tcaaatggct accccaaaac     1080

gatctgcttg gtcatccaaa ggctcgggcg ttcatcacac actccggttc ccatggtatt     1140

tatgaaggaa tatgcaatgg ggttccaatg gtgatgatgc ccttgtttgg tgatcagatg     1200

gacaacgcca agcgcatgga aactcgggga gctggggtga ccctgaatgt cctggaaatg     1260

actgccgatg atttggaaaa cgcccttaaa actgtcatca ataacaagag ttacaaggag     1320

aacatcatgc gcctctccag ccttcacaag gaccgtccta tcgagcctct ggacctggct     1380

gtgttctggg tggagtacgt gatgaggcac aagggggcgc cacacctgcg ccccgccgcc     1440

cacgacctca cctggtacca gtaccactcc ttggacgtga ttggctttct cctggccatc     1500

gtgttgacgg tggtcttcat tgtctataaa agttgtgcct atggctgccg gaaatgcttt     1560

gggggaaagg gtcgagtgaa gaaatcacac aaatccaaga cccac                     1605


<210>  8
<211>  535
<212>  PRT
<213>  Rattus norvegicus

<400>  8

Met Ser Val Val Cys Arg Ser Ser Cys Ser Leu Leu Leu Leu Pro Cys 
1               5                   10                  15      


Leu Leu Leu Cys Val Leu Gly Pro Ser Ala Ser His Ala Gly Lys Leu 
            20                  25                  30          


Leu Val Ile Pro Ile Asp Gly Ser His Trp Leu Ser Met Leu Gly Val 
        35                  40                  45              


Ile Gln Gln Leu Gln Gln Lys Gly His Glu Val Val Val Ile Ala Pro 
    50                  55                  60                  


Glu Ala Ser Ile His Ile Lys Glu Gly Ser Phe Tyr Thr Met Arg Lys 
65                  70                  75                  80  


Tyr Pro Val Pro Phe Gln Asn Glu Asn Val Thr Ala Ala Phe Val Glu 
                85                  90                  95      


Leu Gly Arg Ser Val Phe Asp Gln Asp Pro Phe Leu Leu Arg Val Val 
            100                 105                 110         


Lys Thr Tyr Asn Lys Val Lys Arg Asp Ser Ser Met Leu Leu Ser Gly 
        115                 120                 125             


Cys Ser His Leu Leu His Asn Ala Glu Phe Met Ala Ser Leu Glu Gln 
    130                 135                 140                 


Ser His Phe Asp Ala Leu Leu Thr Asp Pro Phe Leu Pro Cys Gly Ser 
145                 150                 155                 160 


Ile Val Ala Gln Tyr Leu Ser Leu Pro Ala Val Tyr Phe Leu Asn Ala 
                165                 170                 175     


Leu Pro Cys Ser Leu Asp Leu Glu Ala Thr Gln Cys Pro Ala Pro Leu 
            180                 185                 190         


Ser Tyr Val Pro Lys Ser Leu Ser Ser Asn Thr Asp Arg Met Asn Phe 
        195                 200                 205             


Leu Gln Arg Val Lys Asn Met Ile Ile Ala Leu Thr Glu Asn Phe Leu 
    210                 215                 220                 


Cys Arg Val Val Tyr Ser Pro Tyr Gly Ser Leu Ala Thr Glu Ile Leu 
225                 230                 235                 240 


Gln Lys Glu Val Thr Val Lys Asp Leu Leu Ser Pro Ala Ser Ile Trp 
                245                 250                 255     


Leu Met Arg Asn Asp Phe Val Lys Asp Tyr Pro Arg Pro Ile Met Pro 
            260                 265                 270         


Asn Met Val Phe Ile Gly Gly Ile Asn Cys Leu Gln Lys Lys Ala Leu 
        275                 280                 285             


Ser Gln Glu Phe Glu Ala Tyr Val Asn Ala Ser Gly Glu His Gly Ile 
    290                 295                 300                 


Val Val Phe Ser Leu Gly Ser Met Val Ser Glu Ile Pro Glu Lys Lys 
305                 310                 315                 320 


Ala Met Glu Ile Ala Glu Ala Leu Gly Arg Ile Pro Gln Thr Val Leu 
                325                 330                 335     


Trp Arg Tyr Thr Gly Thr Arg Pro Ser Asn Leu Ala Lys Asn Thr Ile 
            340                 345                 350         


Leu Val Lys Trp Leu Pro Gln Asn Asp Leu Leu Gly His Pro Lys Ala 
        355                 360                 365             


Arg Ala Phe Ile Thr His Ser Gly Ser His Gly Ile Tyr Glu Gly Ile 
    370                 375                 380                 


Cys Asn Gly Val Pro Met Val Met Met Pro Leu Phe Gly Asp Gln Met 
385                 390                 395                 400 


Asp Asn Ala Lys Arg Met Glu Thr Arg Gly Ala Gly Val Thr Leu Asn 
                405                 410                 415     


Val Leu Glu Met Thr Ala Asp Asp Leu Glu Asn Ala Leu Lys Thr Val 
            420                 425                 430         


Ile Asn Asn Lys Ser Tyr Lys Glu Asn Ile Met Arg Leu Ser Ser Leu 
        435                 440                 445             


His Lys Asp Arg Pro Ile Glu Pro Leu Asp Leu Ala Val Phe Trp Val 
    450                 455                 460                 


Glu Tyr Val Met Arg His Lys Gly Ala Pro His Leu Arg Pro Ala Ala 
465                 470                 475                 480 


His Asp Leu Thr Trp Tyr Gln Tyr His Ser Leu Asp Val Ile Gly Phe 
                485                 490                 495     


Leu Leu Ala Ile Val Leu Thr Val Val Phe Ile Val Tyr Lys Ser Cys 
            500                 505                 510         


Ala Tyr Gly Cys Arg Lys Cys Phe Gly Gly Lys Gly Arg Val Lys Lys 
        515                 520                 525             


Ser His Lys Ser Lys Thr His 
    530                 535 


<210>  9
<211>  1629
<212>  DNA
<213>  artificial

<220>
<223>  rat UGT1A1 with a C-terminal FLAG tag

<400>  9
atgtccgtgg tgtgccggag ctcatgttcg cttctgcttc ttccgtgcct tctgctgtgt       60

gtgttgggtc cctctgcgtc ccatgctggg aagctgttag tgatccccat agatggcagc      120

cactggctga gtatgctcgg agttattcag cagctccagc aaaaggggca cgaagtggtg      180

gtcatagcac ctgaagcttc gatacacata aaagaaggat cattttacac tatgaggaag      240

taccctgtgc cattccaaaa tgaaaacgtg acagctgctt ttgtggaact tgggcggagt      300

gtctttgatc aagatccttt tctgctgcgt gtggttaaaa catacaacaa agtcaaaagg      360

gactccagta tgctgctgtc tggctgctcc caccttctgc acaatgccga gtttatggcc      420

tctctggaac aaagccactt tgatgctctg ctgacagacc ctttccttcc gtgtggctcc      480

attgtggccc agtacctgtc tctgcctgct gtgtacttct tgaatgcatt gccatgcagc      540

ctggatttgg aagccaccca atgccctgct ccgttgtcct acgtgcccaa gagtttgtcc      600

tcgaacacag atcgcatgaa cttcctgcag cgggtgaaga acatgattat tgctttgaca      660

gagaactttc tatgcagagt ggtttactcc ccctatgggt cacttgccac tgaaatctta      720

cagaaagagg tgactgtcaa ggaccttctg agtcctgcat ctatctggct gatgagaaac      780

gactttgtga aagattaccc caggcccatc atgcccaaca tggtttttat tggtgggata      840

aactgccttc agaaaaaagc cctatcccag gaatttgaag cctatgtcaa cgcctccgga      900

gaacatggca tcgtggtttt ctctttggga tccatggtct cagagattcc agagaagaaa      960

gcgatggaaa ttgctgaggc tttgggcaga attcctcaga cggtcctgtg gcgctacacc     1020

ggaactagac catcgaacct tgcaaagaac actattcttg tcaaatggct accccaaaac     1080

gatctgcttg gtcatccaaa ggctcgggcg ttcatcacac actccggttc ccatggtatt     1140

tatgaaggaa tatgcaatgg ggttccaatg gtgatgatgc ccttgtttgg tgatcagatg     1200

gacaacgcca agcgcatgga aactcgggga gctggggtga ccctgaatgt cctggaaatg     1260

actgccgatg atttggaaaa cgcccttaaa actgtcatca ataacaagag ttacaaggag     1320

aacatcatgc gcctctccag ccttcacaag gaccgtccta tcgagcctct ggacctggct     1380

gtgttctggg tggagtacgt gatgaggcac aagggggcgc cacacctgcg ccccgccgcc     1440

cacgacctca cctggtacca gtaccactcc ttggacgtga ttggctttct cctggccatc     1500

gtgttgacgg tggtcttcat tgtctataaa agttgtgcct atggctgccg gaaatgcttt     1560

gggggaaagg gtcgagtgaa gaaatcacac aaatccaaga cccacgacta caaagacgat     1620

gacgacaag                                                             1629


<210>  10
<211>  543
<212>  PRT
<213>  artificial

<220>
<223>  rat UGT1A1 with a C-terminal FLAG tag

<400>  10

Met Ser Val Val Cys Arg Ser Ser Cys Ser Leu Leu Leu Leu Pro Cys 
1               5                   10                  15      


Leu Leu Leu Cys Val Leu Gly Pro Ser Ala Ser His Ala Gly Lys Leu 
            20                  25                  30          


Leu Val Ile Pro Ile Asp Gly Ser His Trp Leu Ser Met Leu Gly Val 
        35                  40                  45              


Ile Gln Gln Leu Gln Gln Lys Gly His Glu Val Val Val Ile Ala Pro 
    50                  55                  60                  


Glu Ala Ser Ile His Ile Lys Glu Gly Ser Phe Tyr Thr Met Arg Lys 
65                  70                  75                  80  


Tyr Pro Val Pro Phe Gln Asn Glu Asn Val Thr Ala Ala Phe Val Glu 
                85                  90                  95      


Leu Gly Arg Ser Val Phe Asp Gln Asp Pro Phe Leu Leu Arg Val Val 
            100                 105                 110         


Lys Thr Tyr Asn Lys Val Lys Arg Asp Ser Ser Met Leu Leu Ser Gly 
        115                 120                 125             


Cys Ser His Leu Leu His Asn Ala Glu Phe Met Ala Ser Leu Glu Gln 
    130                 135                 140                 


Ser His Phe Asp Ala Leu Leu Thr Asp Pro Phe Leu Pro Cys Gly Ser 
145                 150                 155                 160 


Ile Val Ala Gln Tyr Leu Ser Leu Pro Ala Val Tyr Phe Leu Asn Ala 
                165                 170                 175     


Leu Pro Cys Ser Leu Asp Leu Glu Ala Thr Gln Cys Pro Ala Pro Leu 
            180                 185                 190         


Ser Tyr Val Pro Lys Ser Leu Ser Ser Asn Thr Asp Arg Met Asn Phe 
        195                 200                 205             


Leu Gln Arg Val Lys Asn Met Ile Ile Ala Leu Thr Glu Asn Phe Leu 
    210                 215                 220                 


Cys Arg Val Val Tyr Ser Pro Tyr Gly Ser Leu Ala Thr Glu Ile Leu 
225                 230                 235                 240 


Gln Lys Glu Val Thr Val Lys Asp Leu Leu Ser Pro Ala Ser Ile Trp 
                245                 250                 255     


Leu Met Arg Asn Asp Phe Val Lys Asp Tyr Pro Arg Pro Ile Met Pro 
            260                 265                 270         


Asn Met Val Phe Ile Gly Gly Ile Asn Cys Leu Gln Lys Lys Ala Leu 
        275                 280                 285             


Ser Gln Glu Phe Glu Ala Tyr Val Asn Ala Ser Gly Glu His Gly Ile 
    290                 295                 300                 


Val Val Phe Ser Leu Gly Ser Met Val Ser Glu Ile Pro Glu Lys Lys 
305                 310                 315                 320 


Ala Met Glu Ile Ala Glu Ala Leu Gly Arg Ile Pro Gln Thr Val Leu 
                325                 330                 335     


Trp Arg Tyr Thr Gly Thr Arg Pro Ser Asn Leu Ala Lys Asn Thr Ile 
            340                 345                 350         


Leu Val Lys Trp Leu Pro Gln Asn Asp Leu Leu Gly His Pro Lys Ala 
        355                 360                 365             


Arg Ala Phe Ile Thr His Ser Gly Ser His Gly Ile Tyr Glu Gly Ile 
    370                 375                 380                 


Cys Asn Gly Val Pro Met Val Met Met Pro Leu Phe Gly Asp Gln Met 
385                 390                 395                 400 


Asp Asn Ala Lys Arg Met Glu Thr Arg Gly Ala Gly Val Thr Leu Asn 
                405                 410                 415     


Val Leu Glu Met Thr Ala Asp Asp Leu Glu Asn Ala Leu Lys Thr Val 
            420                 425                 430         


Ile Asn Asn Lys Ser Tyr Lys Glu Asn Ile Met Arg Leu Ser Ser Leu 
        435                 440                 445             


His Lys Asp Arg Pro Ile Glu Pro Leu Asp Leu Ala Val Phe Trp Val 
    450                 455                 460                 


Glu Tyr Val Met Arg His Lys Gly Ala Pro His Leu Arg Pro Ala Ala 
465                 470                 475                 480 


His Asp Leu Thr Trp Tyr Gln Tyr His Ser Leu Asp Val Ile Gly Phe 
                485                 490                 495     


Leu Leu Ala Ile Val Leu Thr Val Val Phe Ile Val Tyr Lys Ser Cys 
            500                 505                 510         


Ala Tyr Gly Cys Arg Lys Cys Phe Gly Gly Lys Gly Arg Val Lys Lys 
        515                 520                 525             


Ser His Lys Ser Lys Thr His Asp Tyr Lys Asp Asp Asp Asp Lys 
    530                 535                 540             


