                               SEQUENCE LISTING

<110> SHORELINE BIOME, LLC
 
<120> METHODS FOR DNA PREPARATION FOR MULTIPLEX HIGH THROUGHPUT 
      TARGETED SEQUENCING

<130> 103929-201

<140> PCT/US2016/065700
<141> 2016-12-09

<150> 15/372,588
<151> 2016-12-08

<150> 62/266,072
<151> 2015-12-11

<160> 65    

<170> PatentIn version 3.5

<210> 1
<211> 575
<212> PRT
<213> Bacillus virus phi29

<400> 1
Met Lys His Met Pro Arg Lys Met Tyr Ser Cys Asp Phe Glu Thr Thr 
1               5                   10                  15      


Thr Lys Val Glu Asp Cys Arg Val Trp Ala Tyr Gly Tyr Met Asn Ile 
            20                  25                  30          


Glu Asp His Ser Glu Tyr Lys Ile Gly Asn Ser Leu Asp Glu Phe Met 
        35                  40                  45              


Ala Trp Val Leu Lys Val Gln Ala Asp Leu Tyr Phe His Asn Leu Lys 
    50                  55                  60                  


Phe Asp Gly Ala Phe Ile Ile Asn Trp Leu Glu Arg Asn Gly Phe Lys 
65                  70                  75                  80  


Trp Ser Ala Asp Gly Leu Pro Asn Thr Tyr Asn Thr Ile Ile Ser Arg 
                85                  90                  95      


Met Gly Gln Trp Tyr Met Ile Asp Ile Cys Leu Gly Tyr Lys Gly Lys 
            100                 105                 110         


Arg Lys Ile His Thr Val Ile Tyr Asp Ser Leu Lys Lys Leu Pro Phe 
        115                 120                 125             


Pro Val Lys Lys Ile Ala Lys Asp Phe Lys Leu Thr Val Leu Lys Gly 
    130                 135                 140                 


Asp Ile Asp Tyr His Lys Glu Arg Pro Val Gly Tyr Lys Ile Thr Pro 
145                 150                 155                 160 


Glu Glu Tyr Ala Tyr Ile Lys Asn Asp Ile Gln Ile Ile Ala Glu Ala 
                165                 170                 175     


Leu Leu Ile Gln Phe Lys Gln Gly Leu Asp Arg Met Thr Ala Gly Ser 
            180                 185                 190         


Asp Ser Leu Lys Gly Phe Lys Asp Ile Ile Thr Thr Lys Lys Phe Lys 
        195                 200                 205             


Lys Val Phe Pro Thr Leu Ser Leu Gly Leu Asp Lys Glu Val Arg Tyr 
    210                 215                 220                 


Ala Tyr Arg Gly Gly Phe Thr Trp Leu Asn Asp Arg Phe Lys Glu Lys 
225                 230                 235                 240 


Glu Ile Gly Glu Gly Met Val Phe Asp Val Asn Ser Leu Tyr Pro Ala 
                245                 250                 255     


Gln Met Tyr Ser Arg Leu Leu Pro Tyr Gly Glu Pro Ile Val Phe Glu 
            260                 265                 270         


Gly Lys Tyr Val Trp Asp Glu Asp Tyr Pro Leu His Ile Gln His Ile 
        275                 280                 285             


Arg Cys Glu Phe Glu Leu Lys Glu Gly Tyr Ile Pro Thr Ile Gln Ile 
    290                 295                 300                 


Lys Arg Ser Arg Phe Tyr Lys Gly Asn Glu Tyr Leu Lys Ser Ser Gly 
305                 310                 315                 320 


Gly Glu Ile Ala Asp Leu Trp Leu Ser Asn Val Asp Leu Glu Leu Met 
                325                 330                 335     


Lys Glu His Tyr Asp Leu Tyr Asn Val Glu Tyr Ile Ser Gly Leu Lys 
            340                 345                 350         


Phe Lys Ala Thr Thr Gly Leu Phe Lys Asp Phe Ile Asp Lys Trp Thr 
        355                 360                 365             


Tyr Ile Lys Thr Thr Ser Glu Gly Ala Ile Lys Gln Leu Ala Lys Leu 
    370                 375                 380                 


Met Leu Asn Ser Leu Tyr Gly Lys Phe Ala Ser Asn Pro Asp Val Thr 
385                 390                 395                 400 


Gly Lys Val Pro Tyr Leu Lys Glu Asn Gly Ala Leu Gly Phe Arg Leu 
                405                 410                 415     


Gly Glu Glu Glu Thr Lys Asp Pro Val Tyr Thr Pro Met Gly Val Phe 
            420                 425                 430         


Ile Thr Ala Trp Ala Arg Tyr Thr Thr Ile Thr Ala Ala Gln Ala Cys 
        435                 440                 445             


Tyr Asp Arg Ile Ile Tyr Cys Asp Thr Asp Ser Ile His Leu Thr Gly 
    450                 455                 460                 


Thr Glu Ile Pro Asp Val Ile Lys Asp Ile Val Asp Pro Lys Lys Leu 
465                 470                 475                 480 


Gly Tyr Trp Ala His Glu Ser Thr Phe Lys Arg Ala Lys Tyr Leu Arg 
                485                 490                 495     


Gln Lys Thr Tyr Ile Gln Asp Ile Tyr Met Lys Glu Val Asp Gly Lys 
            500                 505                 510         


Leu Val Glu Gly Ser Pro Asp Asp Tyr Thr Asp Ile Lys Phe Ser Val 
        515                 520                 525             


Lys Cys Ala Gly Met Thr Asp Lys Ile Lys Lys Glu Val Thr Phe Glu 
    530                 535                 540                 


Asn Phe Lys Val Gly Phe Ser Arg Lys Met Lys Pro Lys Pro Val Gln 
545                 550                 555                 560 


Val Pro Gly Gly Val Val Leu Val Asp Asp Thr Phe Thr Ile Lys 
                565                 570                 575 


<210> 2
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 2
tcagacgatg cgtcatagag tttgatcmtg gctcag                                 36


<210> 3
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 3
cgatcagctg agcgcgtacg gytaccttgt tacgactt                               38


<210> 4
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 4
gcgcgatacg atgactagag tttgatcmtg gctcag                                 36


<210> 5
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 5
atctagcgta gtgatgtacg gytaccttgt tacgactt                               38


<210> 6
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 6
agtgtgtcat gcgtgttacg gytaccttgt tacgactt                               38


<210> 7
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 7
ctatacatga ctctgcagag tttgatcmtg gctcag                                 36


<210> 8
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 8
tgtgtatcag tacatgagag tttgatcmtg gctcag                                 36


<210> 9
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 9
tgcatgcaca gatgcgtacg gytaccttgt tacgactt                               38


<210> 10
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 10
gatctctact atatgcagag tttgatcmtg gctcag                                 36


<210> 11
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 11
gagagacgat cacatatacg gytaccttgt tacgactt                               38


<210> 12
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
agatatgcgc gacacgtacg gytaccttgt tacgactt                               38


<210> 13
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
tactagagta gcactcagag tttgatcmtg gctcag                                 36


<210> 14
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
tatgcatgac tgatattacg gytaccttgt tacgactt                               38


<210> 15
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
acacgcatga cacactagag tttgatcmtg gctcag                                 36


<210> 16
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
tctgtagtgc gtgcgctacg gytaccttgt tacgactt                               38


<210> 17
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 17
gtcgcgacgt cagtgttacg gytaccttgt tacgactt                               38


<210> 18
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 18
acagtctata ctgctgagag tttgatcmtg gctcag                                 36


<210> 19
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 19
atgatgtgct acatctagag tttgatcmtg gctcag                                 36


<210> 20
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 20
ctgcgtgctc tacgacagag tttgatcmtg gctcag                                 36


<210> 21
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 21
cgcgctcagc tgatcgagag tttgatcmtg gctcag                                 36


<210> 22
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 22
gcgcacgcac tacagaagag tttgatcmtg gctcag                                 36


<210> 23
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 23
acactgacgt cgcgacagag tttgatcmtg gctcag                                 36


<210> 24
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 24
atagagactc agagctagag tttgatcmtg gctcag                                 36


<210> 25
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 25
tagatgcgag agtagaagag tttgatcmtg gctcag                                 36


<210> 26
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 26
cacgcacaca cgcgcgagag tttgatcmtg gctcag                                 36


<210> 27
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 27
cgagcacgcg cgtgtgagag tttgatcmtg gctcag                                 36


<210> 28
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 28
gagactctgt gcgcgtagag tttgatcmtg gctcag                                 36


<210> 29
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 29
gctcgactgt gagagaagag tttgatcmtg gctcag                                 36


<210> 30
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 30
tacgactaca tatcagagag tttgatcmtg gctcag                                 36


<210> 31
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 31
gtcagctagt gtcagcagag tttgatcmtg gctcag                                 36


<210> 32
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 32
gtgcagtgat cgatgaagag tttgatcmtg gctcag                                 36


<210> 33
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 33
tgactcgctc atagtcagag tttgatcmtg gctcag                                 36


<210> 34
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 34
atgctgatga cgcgctagag tttgatcmtg gctcag                                 36


<210> 35
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 35
gacagcatct gcgctcagag tttgatcmtg gctcag                                 36


<210> 36
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 36
cgagactgtc gatctctacg gytaccttgt tacgactt                               38


<210> 37
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 37
tcgatatacg acgtgcagag tttgatcmtg gctcag                                 36


<210> 38
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 38
gatcgactcg agcatcagag tttgatcmtg gctcag                                 36


<210> 39
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 39
cgtgcacatc tatagcagag tttgatcmtg gctcag                                 36


<210> 40
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 40
gactgcacat gcacgaagag tttgatcmtg gctcag                                 36


<210> 41
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 41
tcgtcatacg ctctagagag tttgatcmtg gctcag                                 36


<210> 42
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 42
cgactacgta cagtagagag tttgatcmtg gctcag                                 36


<210> 43
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 43
gcgtagacag actacaagag tttgatcmtg gctcag                                 36


<210> 44
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 44
ctgcgcagta cgtgcaagag tttgatcmtg gctcag                                 36


<210> 45
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 45
ctgatgcgcg ctgtacagag tttgatcmtg gctcag                                 36


<210> 46
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 46
gcacatacac gctcacagag tttgatcmtg gctcag                                 36


<210> 47
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 47
agagagagac atgcgcagag tttgatcmtg gctcag                                 36


<210> 48
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 48
actctcgctc tgtagaagag tttgatcmtg gctcag                                 36


<210> 49
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 49
gtacatatgc gtctgtagag tttgatcmtg gctcag                                 36


<210> 50
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 50
tgctcgcagt atcacaagag tttgatcmtg gctcag                                 36


<210> 51
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 51
ctgtgtgtga tagagtagag tttgatcmtg gctcag                                 36


<210> 52
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 52
cagtgagagc gcgataagag tttgatcmtg gctcag                                 36


<210> 53
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 53
catagcgact atcgtgagag tttgatcmtg gctcag                                 36


<210> 54
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 54
catcactacg ctagatagag tttgatcmtg gctcag                                 36


<210> 55
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 55
cgcatctgtg catgcaagag tttgatcmtg gctcag                                 36


<210> 56
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 56
tatgtgatcg tctctcagag tttgatcmtg gctcag                                 36


<210> 57
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 57
gtacacgctg tgactaagag tttgatcmtg gctcag                                 36


<210> 58
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 58
cgtgtcgcgc atatctagag tttgatcmtg gctcag                                 36


<210> 59
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 59
atatcagtca tgcataagag tttgatcmtg gctcag                                 36


<210> 60
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 60
cgctgcgaga gacagtagag tttgatcmtg gctcag                                 36


<210> 61
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 61
tgctctcgtg tactgtagag tttgatcmtg gctcag                                 36


<210> 62
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 62
cactcgtgca cgatgcagag tttgatcmtg gctcag                                 36


<210> 63
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 63
gagatacgct gcagtcagag tttgatcmtg gctcag                                 36


<210> 64
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 64
atctcgagat gtagcgagag tttgatcmtg gctcag                                 36


<210> 65
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 65
acgatcactc gtgtcaagag tttgatcmtg gctcag                                 36


