                         SEQUENCE LISTING

<110>  Eastman Chemicals
       North Carolina State University
       Clendennen, Stephanie  K
       Yingling, Yaroslava G
       Kim, Hoshin
 
<120>  CALB Variants

<130>  E023-0054PCT

<150>  US14961237
<151>  2015-12-07

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  951
<212>  DNA
<213>  Candida Antartica

<400>  1
ttgccatctg gttctgatcc agctttctct caaccaaaat ctgtattaga tgctggattg       60

acatgccagg gtgcttctcc atcatctgtt tctaagccta ttttattggt acctggtaca      120

ggaactactg gtccacagtc attcgattct aattggatac ccttgtctac tcaattaggt      180

tatacaccat gctggatatc tcctccacca tttatgttaa acgacaccca agtaaatacc      240

gagtacatgg ttaacgcaat aacagcattg tacgccggtt caggaaacaa taagttacca      300

gtattgactt ggtctcaggg tggtttagta gcccaatggg gattgacttt cttcccttca      360

atcagatcaa aagttgacag gttgatggca tttgctccag attacaaagg tacagtcttg      420

gctggtccat tagacgcatt agcagtttct gccccatcag tttggcagca gacaacagga      480

tctgccttaa caactgcctt gagaaacgcc ggtggtttga cccagatcgt tccaactacc      540

aacttgtatt ctgccaccga tgaaatcgtc caaccacaag tttctaactc tccattggat      600

tcttcttact tgtttaatgg taaaaatgtt caagcacaag ccgtctgtgg tcctttgttt      660

gtcattgacc atgcaggttc tttgacctct caattttctt atgtcgttgg tagatctgct      720

ttgagatcta ctactggtca agcaagatct gctgattatg gtattactga ttgtaatcca      780

ttgcctgcaa atgatttgac tccagaacaa aaagttgcag ctgctgcttt gttggctcca      840

gctgctgctg ctattgttgc tggtccaaag caaaattgtg aaccagattt gatgccttat      900

gctaggcctt ttgctgttgg taagagaact tgttctggta ttgttactcc a               951


<210>  2
<211>  317
<212>  PRT
<213>  Candida Antartica

<400>  2

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Ala Gly Pro Leu 
    130                 135                 140                 


Asp Ala Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Glu Ile Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Asp His 
    210                 215                 220                 


Ala Gly Ser Leu Thr Ser Gln Phe Ser Tyr Val Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


<210>  3
<211>  951
<212>  DNA
<213>  Artificial

<220>
<223>  Variant of WT CALB

<400>  3
ttgccttcag gttctgaccc agctttctct caaccaaaat ctgtattaga tgctggattg       60

acatgccagg gtgcttctcc atcatctgtt tctaagccta ttttattggt acctggtaca      120

ggaactactg gtccacagtc attcgattct aattggatac ctttgtctac tcaattaggt      180

tatacaccat gctggatatc tcctccacca tttatgttaa acgacaccca agtaaatacc      240

gagtacatgg ttaacgcaat aacagcattg tacgccggtt caggaaacaa taagttacca      300

gtattgactt ggtctcaggg tggtttagta gcccaatggg gattgacttt cttcccttca      360

atcagatcaa aagttgacag gttgatggca tttgctccag attacaaagg tacagtcttg      420

gctggtccat tagacgcatt agcagtttct gccccatcag tttggcagca gacaacagga      480

tctgccttaa caactgcctt gagaaacgcc ggtggtttga cccagatcgt tccaactacc      540

aacttgtatt ctgccaccga tgaaatcgtc caaccacaag tttctaactc tccattggat      600

tcttcttact tgtttaatgg taaaaatgtt caagcacaag ccgtctgtgg tccattgttt      660

gtcatagggc atgctggtac tttgacctct caattttctt atgtcgttgg tagatctgct      720

ttgagatcta ctactggtca agcaagatct gctgattatg gtattactga ttgtaatcca      780

ttgcctgcaa atgatttgac tccagaacaa aaagttgcag ctgccgcttt gttggctcca      840

gctgctgctg ctattgttgc tggtccaaag caaaattgtg aaccagattt gatgccttat      900

gctaggcctt ttgctgttgg taagagaact tgttctggta ttgttactcc a               951


<210>  4
<211>  317
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  4

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Ala Gly Pro Leu 
    130                 135                 140                 


Asp Ala Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Glu Ile Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Gly His 
    210                 215                 220                 


Ala Gly Thr Leu Thr Ser Gln Phe Ser Tyr Val Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


<210>  5
<211>  951
<212>  DNA
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  5
ttgccttcag gttctgaccc agctttctct caaccaaaat ctgtattaga tgctggattg       60

acatgccagg gtgcttctcc atcgtctgtt tctaagccta ttttattggt acctggtaca      120

ggaactactg gtccacagtc attcgattct aattggatac ctttgtctac tcaattaggt      180

tatacaccat gctggatatc ccctccacca tttatgttaa acgacaccca agtaaatacc      240

gagtacatgg ttaacgcaat aacagcattg tacgccggtt caggaaacaa taagttacca      300

gtattgactt ggtctcaggg tggtttagta gcccaatggg gattgacttt cttcccttca      360

atcagatcaa aagttgacag gttgatggca tttgctccag attataaagg tacagtcttg      420

gctggtccat tagacgcatt agcagtttct gccccatcag tttggcagca gacaacagga      480

tctgccttaa caactgcctt gagaaacgcc ggtggtttga cccagatcgt tccaactacc      540

aacttgtatt ctgccaccga tgacatcgtc caaccacaag tttctaactc tccattggat      600

tcttcttact tgtttaatgg taaaaatgtt caagcacaag ccgtctgtgg tccattgttt      660

gtcatagggc atgctggtac tttgacctct caattttctt atgtcgttgg tagatctgct      720

ttgagatcta ctactggtca agcaagatct gctgattatg gtattactga ttgtaatcca      780

ttgcctgcaa atgatttgac tccagaacaa aaagttgcag ctgccgcttt gttggctcca      840

gctgctgctg ctattgttgc tggtccaaag caaaattgtg aaccagattt gatgccttat      900

gctaggcctt ttgctgttgg taagagaact tgttctggta ttgttactcc a               951


<210>  6
<211>  317
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  6

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Ala Gly Pro Leu 
    130                 135                 140                 


Asp Ala Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Asp Ile Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Gly His 
    210                 215                 220                 


Ala Gly Thr Leu Thr Ser Gln Phe Ser Tyr Val Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


<210>  7
<211>  951
<212>  DNA
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  7
ttgccttcag gttctgaccc agctttctct caaccaaaat ctgtattaga tgctggattg       60

acatgccagg gtgcttctcc atcatctgtt tctaagccta ttttattggt acctggtaca      120

ggaactactg gtccacagtc attcgattct aactggatac ctttgtctac tcaattaggt      180

tatacaccat gctggatatc tcctccacca tttatgttaa acgacaccca agtaaatacc      240

gagtacatgg ttaacgcaat aacagcattg tacgccggtt caggaaacaa taagttacca      300

gtattgactt ggtctcaggg tggtttagta gcccaatggg gattgacttt cttcccttca      360

attagatcaa aagttgacag gttgatggca tttgctccag attacaaagg tacagtcttg      420

actggtccat tagacgcatt agctgtttct gccccatcag tttggcagca gacaacagga      480

tctgccttaa caactgcctt gagaaacgcc ggtggtttga cccagatcgt tccaactacc      540

aacttgtatt ctgccaccga tgacatcgtc caaccacaag tttctaactc tccattggat      600

tcttcttact tgtttaatgg taaaaatgtt caagcacaag ccgtctgtgg tccattgttt      660

gtcatagggc atgctggtac tttgacctct caattttctt atgtcgttgg tagatctgct      720

ttgagatcta ctactggtca agcaagatct gctgattatg gtattactga ttgtaatcca      780

ttgcctgcaa atgatttgac tccagaacaa aaagttgcag ctgccgcttt gttggctcca      840

gctgctgctg ctattgttgc tggtccaaag caaaattgtg aaccagattt gatgccttat      900

gctaggcctt ttgctgttgg taagagaact tgttctggta ttgttactcc a               951


<210>  8
<211>  317
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  8

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Thr Gly Pro Leu 
    130                 135                 140                 


Asp Ala Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Asp Ile Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Gly His 
    210                 215                 220                 


Ala Gly Thr Leu Thr Ser Gln Phe Ser Tyr Val Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


<210>  9
<211>  951
<212>  DNA
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  9
ttgccttcag gttctgaccc agctttctct caaccaaaat ctgtattaga tgctggattg       60

acatgccagg gtgcttctcc atcgtctgtt tctaagccta ttttattggt acctggtaca      120

ggaactactg gtccacagtc attcgattct aattggatac ctttgtctac tcaattaggt      180

tatacaccat gctggatatc ccctccacca tttatgttaa acgacaccca agtaaatacc      240

gagtacatgg ttaacgcaat aacagcattg tacgccggtt caggaaacaa taagttacca      300

gtattgactt ggtctcaggg tggtttagta gcccaatggg gattgacttt cttcccttca      360

atcagatcaa aagttgacag gttgatggca tttgctccag attataaagg tacagtcttg      420

acaggtccgt tagacacatt agcagtttct gcaccatcag tttggcagca gacaacagga      480

tctgccttaa caactgcctt gagaaacgcc ggtggtttga cccagatcgt tccaactacc      540

aacttgtatt ctgccaccga tgacatcgtc caaccacaag tttctaactc tccattggat      600

tcttcttact tgtttaatgg taaaaatgtt caagcacaag ccgtctgtgg tccattgttt      660

gtcatagggc atgctggtac tttgacctct caattttctt atgtcgttgg tagatctgct      720

ttgagatcta ctactggtca agcaagatct gctgattatg gtattactga ttgtaatcca      780

ttgcctgcaa atgatttgac tccagaacaa aaagttgcag ctgccgcttt gttggctcca      840

gctgctgctg ctattgttgc tggtccaaag caaaattgtg aaccagattt gatgccttat      900

gctaggcctt ttgctgttgg taagagaact tgttctggta ttgttactcc a               951


<210>  10
<211>  317
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  10

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Thr Gly Pro Leu 
    130                 135                 140                 


Asp Thr Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Asp Ile Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Gly His 
    210                 215                 220                 


Ala Gly Thr Leu Thr Ser Gln Phe Ser Tyr Val Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


<210>  11
<211>  951
<212>  DNA
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  11
ttgccttcag gttctgaccc agctttctct caaccaaaat ctgtattaga tgctggattg       60

acatgccagg gtgcttctcc atcatctgtt tctaagccta ttttattggt acctggtaca      120

ggaactactg gtccacagtc attcgattct aactggatac ctttgtctac tcaattaggt      180

tatacaccat gctggatatc tcctccacca tttatgttaa acgacaccca agtaaatacc      240

gagtacatgg ttaacgcaat aacagcattg tacgccggtt caggaaacaa taagttacca      300

gtattgactt ggtctcaggg tggtttagta gcccaatggg gattgacttt cttcccttca      360

attagatcaa aagttgacag gttgatggca tttgctccag attacaaagg tacagtcttg      420

actggtccat tagacgcatt agctgtttct gccccatcag tttggcagca gacaacagga      480

tctgccttaa caactgcctt gagaaacgcc ggtggtttga cccagatcgt tccaactacc      540

aacttgtatt ctgccaccga tgacgtcgtc caaccacaag tttctaactc tccattggat      600

tcttcttact tgtttaatgg taaaaatgtt caagcacaag ccgtctgtgg tccattgttt      660

gtcatagggc atgctggtac tttgacctct caattttctt atgtcgttgg tagatctgct      720

ctgagatcta ctactggtca agcaagatct gctgattatg gtattactga ttgtaatcca      780

ttgcctgcaa atgatttgac tccagaacaa aaagttgcag ctgccgcttt gttggctcca      840

gctgctgctg ctattgttgc tggtccaaag caaaattgtg aaccagattt gatgccttat      900

gctaggcctt ttgctgttgg taagagaact tgttctggta ttgttactcc a               951


<210>  12
<211>  317
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  12

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Thr Gly Pro Leu 
    130                 135                 140                 


Asp Ala Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Asp Val Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Gly His 
    210                 215                 220                 


Ala Gly Thr Leu Thr Ser Gln Phe Ser Tyr Val Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


<210>  13
<211>  951
<212>  DNA
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  13
ttgccttcag gttctgaccc ggctttctct caaccaaaat ctgtattaga tgctggattg       60

acatgccagg gtgcttctcc atcgtctgtt tctaagccta ttttattggt acctggtaca      120

ggaactactg gtccacagtc attcgattct aattggatac ctttgtctac tcaattaggt      180

tatacaccat gctggatatc ccctccacca tttatgttaa acgacaccca agtaaatacc      240

gagtacatgg ttaacgcaat aacagcattg tacgccggtt caggaaacaa taagttacca      300

gtattgactt ggtctcaggg tggtttagta gcccaatggg gattgacttt cttcccttca      360

attagatcaa aagttgacag gttgatggca tttgctccag attacaaagg tacagtcttg      420

actggtccat tagacgcatt agcagtttct gccccatcag tttggcagca gacaacagga      480

tctgccctaa caactgcctt gagaaacgcc ggtggtttga cccagatcgt tccaactacc      540

aacttgtatt ctgccaccga tgacatcgtc caaccacaag tttctaactc tccattggat      600

tcttcttact tgtttaatgg taaaaatgtt caagcacaag ccgtctgtgg tccattgttt      660

gtcatagggc atgctggtac tttgacctct caattttctt atgccgttgg tagatccgct      720

ttgagatcta ctactggtca agcaagatct gctgattatg gtattactga ttgtaatcca      780

ttgcctgcaa atgatttgac tccagaacaa aaagttgcag ctgccgcttt gttggctcca      840

gctgctgctg ctattgttgc tggtccaaag caaaattgtg aaccagattt gatgccttat      900

gctaggcctt ttgctgttgg taagagaact tgttctggta ttgttactcc a               951


<210>  14
<211>  317
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  14

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Thr Gly Pro Leu 
    130                 135                 140                 


Asp Ala Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Asp Ile Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Gly His 
    210                 215                 220                 


Ala Gly Thr Leu Thr Ser Gln Phe Ser Tyr Ala Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


<210>  15
<211>  951
<212>  DNA
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  15
ttgccttcag gttctgaccc agctttctct caaccaaaat ctgtattaga tgctggattg       60

acatgccagg gtgcttctcc atcatctgtt tctaagccta ttttattggt acctggtaca      120

ggaactactg gtccacagtc attcgattct aattggatac ctttgtctac tcaattaggt      180

tatacaccat gctggatatc tcctccacca tttatgttaa acgacaccca agtaaatacc      240

gagtacatgg ttaacgcaat aacagcattg tacgccggtt caggaaacaa taagttacca      300

gtattgactt ggtctcaggg tggtttagta gcccaatggg gattgacttt cttcccttca      360

atcagatcaa aagttgacag gttgatggca tttgctccag attataaagg tacagtcttg      420

acgggtccgt tagacacatt agcagtttct gctccatcag tttggcagca gacaacagga      480

tctgccttaa caactgcctt gagaaacgcc ggtggtttga cccagatcgt tccaactacc      540

aacttgtatt ctgccaccga tgacatcgtc caaccacaag tttctaactc tccattggat      600

tcttcttact tgtttaatgg taaaaatgtt caagcacaag ccgtctgtgg tccattgttt      660

gtcatagggc atgctggtac tttgacctct caattttctt atgccgttgg tagatccgct      720

ttgagatcta ctactggtca agcaagatct gctgattatg gtattactga ttgtaatcca      780

ttgcctgcaa atgatttgac tccagaacaa aaagttgcag ctgccgcttt gttggctcca      840

gctgctgctg ctattgttgc tggtccaaag caaaattgtg aaccagattt gatgccttat      900

gctaggcctt ttgctgttgg taagagaact tgttctggta ttgttactcc a               951


<210>  16
<211>  317
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  16

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Thr Gly Pro Leu 
    130                 135                 140                 


Asp Thr Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Asp Ile Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Gly His 
    210                 215                 220                 


Ala Gly Thr Leu Thr Ser Gln Phe Ser Tyr Ala Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


<210>  17
<211>  951
<212>  DNA
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  17
ttgccttcag gttctgaccc ggctttctct caaccaaaat ctgtattaga tgctggattg       60

acatgccagg gtgcttctcc atcgtctgtt tctaagccta ttttattggt acctggtaca      120

ggaactactg gtccacagtc attcgattct aattggatac ctttgtctac tcaattaggt      180

tatacaccat gctggatatc ccctccacca tttatgttaa acgacaccca agtaaatacc      240

gagtacatgg ttaacgcaat aacagcattg tacgccggtt caggaaacaa taagttacca      300

gtattgactt ggtctcaggg tggtttagta gcccaatggg gattgacttt cttcccttca      360

attagatcaa aagttgacag gttgatggca tttgctccag attacaaagg tacagtcttg      420

accggtccgt tagacacatt agcagtttct gccccatcag tttggcagca gacaacagga      480

tctgccttaa caactgcctt gagaaacgcc ggtggtttga cccagatcgt tccaactacc      540

aacttgtatt ctgccaccga tgacgtcgtc caaccacaag tttctaactc tccattggat      600

tcttcttact tgtttaatgg taaaaatgtt caagcacaag ccgtctgtgg tccattgttt      660

gtcatagggc atgctggtac tttgacctct caattttctt atgtcgttgg tagatctgct      720

ctgagatcta ctactggtca agcaagatct gctgattatg gtattactga ttgtaatccg      780

ttgcctgcaa atgatttgac tccagaacaa aaagttgcag ctgccgcttt gttggctcca      840

gctgctgctg ctattgttgc tggtccaaag caaaattgtg aaccagattt gatgccttat      900

gctaggcctt ttgctgttgg taagagaact tgttctggta ttgttactcc a               951


<210>  18
<211>  317
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  18

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Thr Gly Pro Leu 
    130                 135                 140                 


Asp Thr Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Asp Val Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Gly His 
    210                 215                 220                 


Ala Gly Thr Leu Thr Ser Gln Phe Ser Tyr Val Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


<210>  19
<211>  951
<212>  DNA
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  19
ttgccttcag gttctgaccc ggctttctct caaccaaaat ctgtattaga tgctggattg       60

acatgccagg gtgcttctcc atcgtctgtt tctaagccta ttttattggt acctggtaca      120

ggaactactg gtccacagtc attcgattct aattggatac ctttgtctac tcaattaggt      180

tatacaccat gctggatatc ccctccacca tttatgttaa acgacaccca agtaaatacc      240

gagtacatgg ttaacgcaat aacagcattg tacgccggtt caggaaacaa taagttacca      300

gtattgactt ggtctcaggg tggtttagta gcccaatggg gattgacttt cttcccttca      360

attagatcaa aagttgacag gttgatggca tttgctccag attacaaagg tacagtcttg      420

actggtccat tagacgcatt agcagtttct gccccatcag tttggcagca gacaacagga      480

tctgccctaa caactgcctt gagaaacgcc ggtggtttga cccagatcgt tccaactacc      540

aacttgtatt ctgccaccga tgacgtcgtc caaccacaag tttctaactc tccattggat      600

tcttcttact tgtttaatgg taaaaatgtt caagcacaag ccgtctgtgg tccattgttt      660

gtcatagggc atgctggtac tttgacctct caattttctt atgccgttgg tagatccgct      720

ttgagatcta ctactggtca agcaagatct gctgattatg gtattactga ttgtaatcca      780

ttgcctgcaa atgatttgac tccagaacaa aaagttgcag ctgccgcttt gttggctcca      840

gctgctgctg ctattgttgc tggtccaaag caaaattgtg aaccagattt gatgccttat      900

gctaggcctt ttgctgttgg taagagaact tgttctggta ttgttactcc a               951


<210>  20
<211>  317
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  20

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Thr Gly Pro Leu 
    130                 135                 140                 


Asp Ala Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Asp Val Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Gly His 
    210                 215                 220                 


Ala Gly Thr Leu Thr Ser Gln Phe Ser Tyr Ala Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


<210>  21
<211>  951
<212>  DNA
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  21
ttgccttcag gttctgaccc ggctttctct caaccaaaat ctgtattaga tgctggattg       60

acatgccagg gtgcttctcc atcgtctgtt tctaagccta ttttattggt acctggtaca      120

ggaactactg gtccacagtc attcgattct aattggatac ctttgtctac tcaattaggt      180

tatacaccat gctggatatc ccctccacca tttatgttaa acgacaccca agtaaatacc      240

gagtacatgg ttaacgcaat aacagcattg tacgccggtt caggaaacaa taagttacca      300

gtattgactt ggtctcaggg tggtttagta gcccaatggg gattgacttt cttcccttca      360

attagatcaa aagttgacag gttgatggca tttgctccag attacaaagg tacagtcttg      420

actggtccgt tagacacatt agcagtttct gccccatcag tttggcagca gacaacagga      480

tctgccttaa caactgcctt gagaaacgcc ggtggtttga cccagatcgt tccaactacc      540

aacttgtatt ctgccaccga tgacgtcgtc caaccacaag tttctaactc tccattggat      600

tcttcttact tgtttaatgg taaaaatgtt caagcacaag ccgtctgtgg tccattgttt      660

gtcatagggc atgctggtac tttgacctct caattttctt atgccgttgg tagatccgct      720

ttgagatcta ctactggtca agcaagatct gctgattatg gtattactga ttgtaatcca      780

ttgcctgcaa atgatttgac tccagaacaa aaagttgcag ctgccgcttt gttggctcca      840

gctgctgctg ctattgttgc tggtccaaag caaaattgtg aaccagattt gatgccttat      900

gctaggcctt ttgctgttgg taagagaact tgttctggta ttgttactcc a               951


<210>  22
<211>  317
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  22

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Thr Gly Pro Leu 
    130                 135                 140                 


Asp Thr Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Asp Val Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Gly His 
    210                 215                 220                 


Ala Gly Thr Leu Thr Ser Gln Phe Ser Tyr Ala Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


<210>  23
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  23
gcttggtacc agctattgta acataatcg                                         29


<210>  24
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  24
cagacaaaga tctccatgga cgcgtg                                            26


<210>  25
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  25
ggtaagagaa cttgttctgg tattgttact ccataataac cc                          42


<210>  26
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  26
cagcgtgcac ataagcacaa gtctgaacga aactgtccgc                             40


<210>  27
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (17)..(19)
<223>  n is a, c, g, or t

<400>  27
gtattctgcc accgatnnna tcgtccaacc acaagtttct                             40


<210>  28
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  28
ccattaaaca agtaagaaga atccaatgga gagttag                                37


<210>  29
<211>  317
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of CA:LB

<400>  29

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Ala Gly Pro Leu 
    130                 135                 140                 


Asp Ala Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Glu Ile Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Gly His 
    210                 215                 220                 


Ala Gly Ser Leu Thr Ser Gln Phe Ser Tyr Val Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


<210>  30
<211>  317
<212>  PRT
<213>  Artificial

<220>
<223>  Variant of CALB

<400>  30

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Ala Gly Pro Leu 
    130                 135                 140                 


Asp Ala Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Glu Ile Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Asp His 
    210                 215                 220                 


Ala Gly Thr Leu Thr Ser Gln Phe Ser Tyr Val Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


