                         SEQUENCE LISTING

<110>  Consortium fr elektrochemische Industrie - Wacker Chemie 
       AG
       Bornhvd, Dr. Carsten
       Thn, Dr. Marcel
 
<120>  Mikroorganismenstamm und Verfahren zur antibiotikafreien, 
       fermentativen Herstellung von niedermolekularen Substanzen und 
       Proteinen

<130>  Co11517

<150>  Co11517
<151>  2015-08-27

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  726
<212>  DNA
<213>  Escherichia coli


<220>
<221>  gene
<222>  (1)..(726)
<223>  pyrH

<400>  1
atggctacca atgcaaaacc cgtctataaa cgcattctgc ttaagttgag tggcgaagct       60

ctgcagggca ctgaaggctt cggtattgat gcaagcatac tggatcgtat ggctcaggaa      120

atcaaagaac tggttgaact gggtattcag gttggtgtgg tgattggtgg gggtaacctg      180

ttccgtggcg ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga ccacatgggg      240

atgctggcga ccgtaatgaa cggcctggca atgcgtgatg cactgcaccg cgcctatgtg      300

aacgctcgtc tgatgtccgc tattccattg aatggcgtgt gcgacagcta cagctgggca      360

gaagctatca gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg tacaggtaac      420

ccgttcttta ccaccgactc agcagcttgc ctgcgtggta tcgaaattga agccgatgtg      480

gtgctgaaag caaccaaagt tgacggcgtg tttaccgctg atccggcgaa agatccaacc      540

gcaaccatgt acgagcaact gacttacagc gaagtgctgg aaaaagagct gaaagtcatg      600

gacctggcgg ccttcacgct ggctcgtgac cataaattac cgattcgtgt tttcaatatg      660

aacaaaccgg gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac tttaatcacg      720

gaataa                                                                 726


<210>  2
<211>  241
<212>  PRT
<213>  Escherichia coli


<220>
<221>  PEPTIDE
<222>  (1)..(241)

<400>  2

Met Ala Thr Asn Ala Lys Pro Val Tyr Lys Arg Ile Leu Leu Lys Leu 
1               5                   10                  15      


Ser Gly Glu Ala Leu Gln Gly Thr Glu Gly Phe Gly Ile Asp Ala Ser 
            20                  25                  30          


Ile Leu Asp Arg Met Ala Gln Glu Ile Lys Glu Leu Val Glu Leu Gly 
        35                  40                  45              


Ile Gln Val Gly Val Val Ile Gly Gly Gly Asn Leu Phe Arg Gly Ala 
    50                  55                  60                  


Gly Leu Ala Lys Ala Gly Met Asn Arg Val Val Gly Asp His Met Gly 
65                  70                  75                  80  


Met Leu Ala Thr Val Met Asn Gly Leu Ala Met Arg Asp Ala Leu His 
                85                  90                  95      


Arg Ala Tyr Val Asn Ala Arg Leu Met Ser Ala Ile Pro Leu Asn Gly 
            100                 105                 110         


Val Cys Asp Ser Tyr Ser Trp Ala Glu Ala Ile Ser Leu Leu Arg Asn 
        115                 120                 125             


Asn Arg Val Val Ile Leu Ser Ala Gly Thr Gly Asn Pro Phe Phe Thr 
    130                 135                 140                 


Thr Asp Ser Ala Ala Cys Leu Arg Gly Ile Glu Ile Glu Ala Asp Val 
145                 150                 155                 160 


Val Leu Lys Ala Thr Lys Val Asp Gly Val Phe Thr Ala Asp Pro Ala 
                165                 170                 175     


Lys Asp Pro Thr Ala Thr Met Tyr Glu Gln Leu Thr Tyr Ser Glu Val 
            180                 185                 190         


Leu Glu Lys Glu Leu Lys Val Met Asp Leu Ala Ala Phe Thr Leu Ala 
        195                 200                 205             


Arg Asp His Lys Leu Pro Ile Arg Val Phe Asn Met Asn Lys Pro Gly 
    210                 215                 220                 


Ala Leu Arg Arg Val Val Met Gly Glu Lys Glu Gly Thr Leu Ile Thr 
225                 230                 235                 240 


Glu 
    


<210>  3
<211>  738
<212>  DNA
<213>  Escherichia coli


<220>
<221>  gene
<222>  (1)..(738)
<223>  plsC

<400>  3
atgctatata tctttcgtct tattattacc gtgatttaca gcatcttagt ctgtgtattc       60

ggctccattt actgcctttt cagcccgcgt aacccgaaac atgtggccac ctttgggcat      120

atgtttggcc gtcttgcgcc gctgtttggc ctgaaagttg agtgccgtaa acctacagac      180

gctgaaagct acggcaatgc tatctatatc gctaaccacc agaacaacta tgacatggtg      240

acagcatcga acatcgtgca accgccgacg gtgacggtag gtaaaaagag cttgctgtgg      300

atccccttct tcgggcagtt gtactggtta accggcaact tattgatcga cagaaacaat      360

cgcactaaag ctcacggcac cattgcggaa gtagtgaatc acttcaaaaa acgccgtatt      420

tccatctgga tgttcccgga aggaacccgc agccgtggtc gcggcctgct accgttcaag      480

actggagcat ttcacgcggc aattgcggcg ggcgtcccga ttattcccgt gtgcgtctct      540

acaacttcga ataagattaa tcttaatcga ctgcacaacg gtctggtgat tgtcgaaatg      600

ctgccgccaa ttgacgtcag tcagtatggc aaagatcagg ttcgtgagct ggctgcccat      660

tgtcgttcga taatggaaca aaaaatcgcc gagctcgata aagaagtcgc agaacgcgaa      720

gccgccggaa aagtttaa                                                    738


<210>  4
<211>  245
<212>  PRT
<213>  Escherichia coli


<220>
<221>  PEPTIDE
<222>  (1)..(245)

<220>
<221>  PEPTIDE
<222>  (1)..(245)
<223>  PlsC

<400>  4

Met Leu Tyr Ile Phe Arg Leu Ile Ile Thr Val Ile Tyr Ser Ile Leu 
1               5                   10                  15      


Val Cys Val Phe Gly Ser Ile Tyr Cys Leu Phe Ser Pro Arg Asn Pro 
            20                  25                  30          


Lys His Val Ala Thr Phe Gly His Met Phe Gly Arg Leu Ala Pro Leu 
        35                  40                  45              


Phe Gly Leu Lys Val Glu Cys Arg Lys Pro Thr Asp Ala Glu Ser Tyr 
    50                  55                  60                  


Gly Asn Ala Ile Tyr Ile Ala Asn His Gln Asn Asn Tyr Asp Met Val 
65                  70                  75                  80  


Thr Ala Ser Asn Ile Val Gln Pro Pro Thr Val Thr Val Gly Lys Lys 
                85                  90                  95      


Ser Leu Leu Trp Ile Pro Phe Phe Gly Gln Leu Tyr Trp Leu Thr Gly 
            100                 105                 110         


Asn Leu Leu Ile Asp Arg Asn Asn Arg Thr Lys Ala His Gly Thr Ile 
        115                 120                 125             


Ala Glu Val Val Asn His Phe Lys Lys Arg Arg Ile Ser Ile Trp Met 
    130                 135                 140                 


Phe Pro Glu Gly Thr Arg Ser Arg Gly Arg Gly Leu Leu Pro Phe Lys 
145                 150                 155                 160 


Thr Gly Ala Phe His Ala Ala Ile Ala Ala Gly Val Pro Ile Ile Pro 
                165                 170                 175     


Val Cys Val Ser Thr Thr Ser Asn Lys Ile Asn Leu Asn Arg Leu His 
            180                 185                 190         


Asn Gly Leu Val Ile Val Glu Met Leu Pro Pro Ile Asp Val Ser Gln 
        195                 200                 205             


Tyr Gly Lys Asp Gln Val Arg Glu Leu Ala Ala His Cys Arg Ser Ile 
    210                 215                 220                 


Met Glu Gln Lys Ile Ala Glu Leu Asp Lys Glu Val Ala Glu Arg Glu 
225                 230                 235                 240 


Ala Ala Gly Lys Val 
                245 


<210>  5
<211>  40
<212>  DNA
<213>  synthetisches Oligonukleotid


<220>
<221>  misc_feature
<223>  Oligonukleotid: pyrH-NcoI-fw

<400>  5
ccccccatgg ccatcttgta aattcagcta acccttgtgg                             40


<210>  6
<211>  40
<212>  DNA
<213>  synthetisches Oligonukleotid


<220>
<221>  misc_feature
<223>  Oligonukleotid: pyrH-NcoI-rev

<400>  6
ggggccatgg atcctcacgt acttttgtac gctccggttg                             40


<210>  7
<211>  37
<212>  DNA
<213>  synthetisches Oligonukleotid


<220>
<221>  misc_feature
<223>  Oligonukleotid: plsC-NcoI-fw

<400>  7
ccccccatgg atttgctcca tgtaaaactg gctaaag                                37


<210>  8
<211>  40
<212>  DNA
<213>  synthetisches Oligonukleotid


<220>
<221>  misc_feature
<223>  Oligonukleotid: plsC-NcoI-rev

<400>  8
ggggccatgg tgaaaccgtt gtttattcat gcgttgcgat                             40


<210>  9
<211>  71
<212>  DNA
<213>  synthetisches Oligonukleotid


<220>
<221>  misc_feature
<223>  synthetisches Oligonukleotid: pyrH-fw

<220>
<221>  misc_feature
<223>  Oligonukleotid: pyrH-fw

<400>  9
cagtcttaat tatcaaaaag gagccgcctg agggcggctt ctttttgtgc cgtgtaggct       60

ggagctgctt c                                                            71


<210>  10
<211>  70
<212>  DNA
<213>  synthetisches Oligonukleotid


<220>
<221>  misc_feature
<223>  Oligonukleotid: pyrH-rev

<400>  10
gtaatcgtct ggattattag gctattttat ttgccatttt ggccccgggc atgggaatta       60

gccatggtcc                                                              70


<210>  11
<211>  30
<212>  DNA
<213>  synthetisches Oligonukleotid


<220>
<221>  misc_feature
<223>  Oligonukleotid: pyrH-check-for

<400>  11
gcataacgct gaagtgactg gcttcatccg                                        30


<210>  12
<211>  30
<212>  DNA
<213>  synthetisches Oligonukleotid


<220>
<221>  misc_feature
<223>  Oligonukleotid: pyrH-check-rev

<400>  12
cagaccagac agtcacacac agtggaagtg                                        30


<210>  13
<211>  71
<212>  DNA
<213>  synthetisches Oligonukleotid


<220>
<221>  misc_feature
<223>  Oligonukleotid: plsC-fw

<400>  13
ctgacgtcaa ttggcggcag catttcgaca atcaccagac cgttgtgcag cgtgtaggct       60

ggagctgctt c                                                            71


<210>  14
<211>  71
<212>  DNA
<213>  synthetisches Oligonukleotid


<220>
<221>  misc_feature
<223>  Oligonukleotid: plsC-rev

<400>  14
ctgacgtcaa ttggcggcag catttcgaca atcaccagac cgttgtgcag cgtgtaggct       60

ggagctgctt c                                                            71


<210>  15
<211>  30
<212>  DNA
<213>  synthetisches Oligonukleotid


<220>
<221>  misc_feature
<223>  Oligonukleotid: plsC-check-for

<400>  15
cgagattcaa gtagcggcgg aacgggtagc                                        30


<210>  16
<211>  30
<212>  DNA
<213>  synthetisches Oligonukleotid


<220>
<221>  misc_feature
<223>  Oligonukleotid: plsC-check-rev

<400>  16
gttaagtcgt catccggcag cgtattcaac                                        30


<210>  17
<211>  4487
<212>  DNA
<213>  Plasmid


<220>
<221>  misc_feature
<222>  (1)..(4308)
<223>  Plasmid pMT1

<400>  17
tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg       60

gttctggcaa atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt      120

ggaattgtga gcggataaca atttcacaca ggaaacagaa ttcccgggga tccgtcgacc      180

tgcagccaag cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa      240

atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt      300

cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg      360

gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga      420

aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa      480

atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac      540

gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt      600

ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc      660

atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt      720

cggtcgccgc atacactatt ctcagaatga cttggttgag tccatggcgg ccgccgatct      780

aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc      840

actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc      900

gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg      960

atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa     1020

atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc     1080

ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt     1140

gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa     1200

cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc     1260

tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc     1320

cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct     1380

ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat     1440

gctcgtcagg ggggcggagc ctatggaaaa acgattctca tgtttgacag cttatcatcg     1500

ataagctcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg     1560

acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt     1620

gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca     1680

ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag     1740

ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc     1800

cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg     1860

aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat     1920

cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc     1980

gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt     2040

ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat     2100

tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc     2160

gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt     2220

cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa     2280

ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc     2340

cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat     2400

ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct     2460

catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc     2520

agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcca     2580

caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag     2640

caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa     2700

ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg     2760

gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc     2820

cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg     2880

actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg     2940

tcaaacatgg caggcctccc ccagaagtcg cttgcggtat tcggaatctt gcacgccctc     3000

gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc     3060

gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgaa cgccagcaag     3120

acgtagccca gcgcgtcggc cagcttgcaa ttcgcgctaa cttacattaa ttgcgttgcg     3180

ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca     3240

acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg     3300

agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt     3360

ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttgac ggcgggatat     3420

aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca     3480

gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca     3540

tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg     3600

cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat     3660

gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga     3720

tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg     3780

agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat     3840

tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca     3900

gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc     3960

ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg     4020

ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca     4080

acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca     4140

tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc     4200

gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt     4260

tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg     4320

ttttgcacca ttcgatggtg tcaacgtaaa tgcatgccgc ttcgccttcg cgcgcgaatt     4380

gcaagctgat ccgggcttat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc     4440

atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataat                   4487


