                         SEQUENCE LISTING

<110>  Trianni, Inc.
 
<120>  Enhanced Immunoglobulin Diversity

<130>  TRIA010PCT

<140>  Filed herewith
<141>  2016-11-30

<150>  62/262,757
<151>  2015-12-03

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  625
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Element 512 in Figure 5, including splice acceptor, mouse Itgb7 
       exon 15 with stop codon, mouse Itgb7 intron, mouse Itgb7 exon 15 
       with stop codon and 3' untranslated region, and polyadenylation 
       sequence

<400>  1
agggagtgga tcacacccgt gccatcatac tgggctgcac agggggcatc gtggcagtgg       60

gactagggct ggttctggct tactgactct ctgtggaaat ctacgaccga cgggagtaca      120

ggcgctttga gaaggagcag cagcaactca actggaagca ggtgaggcca gcgactgctg      180

ccacaggctg ggctttcctg gtgacgtctc ttaactttct gtatccctaa cacataacca      240

gctctaaagc ttccccgtgc aagtccctcc ctcggcataa ccaggcctcg gagatctggc      300

ctcgtggggc aggtagtggg gagagcctga tagttttcct tactgtgtgc aatgttttcc      360

cacaggacaa caatcctctc tacaaaagtg cgatcacaac cactgtcaac ccccgcttcc      420

aagggacaaa cggtcggtcg ccatccctct ctctgaccag ggaagcagac tgacttagga      480

ttcttgtctt ggaggacagt ggagatagaa gggcagggca gcgtctgtca ggcaaagatg      540

ctgccaccgc tgagattttt cagagtgacc ttcagagggc agcagccatt cccaccacac      600

gaagggctgg tccttccata ataaa                                            625


<210>  2
<211>  760
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Element 513 in Figure 5: splice acceptor, linker, PTV1-2A 
       peptide, mouse Bcl2 open reading frame, 3' untranslated region, 
       polyadenylation sequence

<400>  2
cagggggatc cggagccacg aacttctctc tgttaaagca agcaggagac gtggaagaaa       60

accccggtcc tatggcgcaa gccgggagaa cagggtatga taaccgggag atcgtgatga      120

agtacataca ttataagctg tcacagaggg gctacgagtg ggatgctgga gatgcggacg      180

cggcgcccct gggggctgcc cccacccctg gcatcttctc cttccagcct gagagcaacc      240

caatgcccgc tgtgcaccgg gacatggctg ccaggacgtc tcctctcagg cccctcgttg      300

ccaccgctgg gcctgcgctc agccctgtgc cacctgtggt ccatctgacc ctccgccggg      360

ctggggatga cttctctcgt cgctaccgtc gtgacttcgc agagatgtcc agtcagctgc      420

acctgacgcc cttcaccgcg aggggacgct ttgccacggt ggtggaggaa ctcttcaggg      480

atggggtgaa ctgggggagg attgtggcct tctttgagtt cggtggggtc atgtgtgtgg      540

agagcgtcaa cagggagatg tcacccctgg tggacaacat cgccctgtgg atgactgagt      600

acctgaaccg gcatctgcac acctggatcc aggataacgg aggctgggta ggtgcatgtc      660

tggttgaatg agtctgggct ttgatctcaa ggccaagatg cgcaggttgg ggtgtgagtg      720

gattctgggt caaaatgggc cattgagcag atgaaataaa                            760


<210>  3
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Element 610 in Figure 6: promoter and other regulatory sequences,
       exon 1, intron, exon 2.

<400>  3
ggatccagag ttaggacttg gactgactca ggaggactct agtttcttct tctccagctg       60

gaatgtcctt atgtaagaaa agccttgcct catgagtatg caaatcatgt gcgactgtga      120

tgattaatat agggatatcc acaccaaaca tcatatgagc cctatcttct ctacagacac      180

tgaatctcaa ggtccttaca atgaaatgca gctgggttat cttcttcctg atggcagtgg      240

ttacagggaa ggggctccca agcccaaact tgaggtgtcc ataaactctc tgtgacagtg      300

gcaatccact ttgcctttct ttctacaggg gtcaattcag aggttcagct gcagcagtct      360

ggggcagagc ttgtgaggcc aggggcctca gtcaagttgt cctgcacggc ttctggcttc      420

aacattaaag acacctatat gcactgggtg aagcagaggc ctgaacaggg cctggagtgg      480

attggaagga ttgatcctgc gaatggtaat actaaatatg acccgaagtt ccagggcaag      540

gccactataa cagcagacac atccaccaac acagcctacc tgcagctcag cagcctgaca      600

tctgaggaca ctgccgtcta ttactgtgct agatactata ggtaccctta ctatgctatg      660

gactactggg gtcaaggaac ctcagtcacc gtctcctca                             699


<210>  4
<211>  139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Translated amino acid sequence of element 610 in Figure 6

<400>  4

Met Lys Cys Ser Trp Val Ile Phe Phe Leu Met Ala Val Val Thr Gly 
1               5                   10                  15      


Val Asn Ser Glu Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile 
        35                  40                  45              


Lys Asp Thr Tyr Met His Trp Val Lys Gln Arg Pro Glu Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Arg Ile Asp Pro Ala Asn Gly Asn Thr Lys Tyr Asp 
65                  70                  75                  80  


Pro Lys Phe Gln Gly Lys Ala Thr Ile Thr Ala Asp Thr Ser Thr Asn 
                85                  90                  95      


Thr Ala Tyr Leu Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Tyr Tyr Arg Tyr Pro Tyr Tyr Ala Met Asp Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
    130                 135                 


<210>  5
<211>  627
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of DNA cassette, element 611 in Figure 6: splice 
       acceptor, mouse Itgb7 exon 15 with stop codon, mouse Itgb7 
       intron, mouse Itgb7 exon 15 with stop codon followed by 3' 
       untranslated region, polyadenylation sequence

<400>  5
cagagggagt ggatcacacc cgtgccatca tactgggctg cacagggggc atcgtggcag       60

tgggactagg gctggttctg gcttactgac tctctgtgga aatctacgac cgacgggagt      120

acaggcgctt tgagaaggag cagcagcaac tcaactggaa gcaggtgagg ccagcgactg      180

ctgccacagg ctgggctttc ctggtgacgt ctcttaactt tctgtatccc taacacataa      240

ccagctctaa agcttccccg tgcaagtccc tccctcggca taaccaggcc tcggagatct      300

ggcctcgtgg ggcaggtagt ggggagagcc tgatagtttt ccttactgtg tgcaatgttt      360

tcccacagga caacaatcct ctctacaaaa gtgcgatcac aaccactgtc aacccccgct      420

tccaagggac aaacggtcgg tcgccatccc tctctctgac cagggaagca gactgactta      480

ggattcttgt cttggaggac agtggagata gaagggcagg gcagcgtctg tcaggcaaag      540

atgctgccac cgctgagatt tttcagagtg accttcagag ggcagcagcc attcccacca      600

cacgaagggc tggtccttcc ataataa                                          627


<210>  6
<211>  1393
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of DNA cassette, element 612 in Figure 6: splice 
       acceptor, PDGFR transmembrane domain, linker, PTV1-2A peptide; 
       Cre recombinase coding sequence, 3' untranslated region with 
       poly-A signal

<400>  6
caggggctgt gggccaggac acgcaggagg tcatcgtggt gccacactcc ttgcccttta       60

aggtggtggt gatctcagcc atcctggccc tggtggtgct caccatcatc tcccttatca      120

tcctcatcat gctttggcag aagaagccac gtatgtccaa tttactgacc gtacaccaaa      180

atttgcctgc attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca      240

tgttcaggga tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc      300

ggtcgtgggc ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag      360

atgttcgcga ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc      420

aacatttggg ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca      480

gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac      540

gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg      600

aaaatagcga tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca      660

ccctgttacg tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg      720

gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag      780

agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg      840

tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc      900

catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc      960

gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca     1020

gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca     1080

tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata     1140

gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga ttagagacaa aggtcctgag     1200

acgccaccac cagctcccca gctccatcct atcttccctt ctaaggtctt ggaggcttcc     1260

ccacaagcga cctaccactg ttgcggtgct ccaaacctcc tccccacctc cttctcctcc     1320

tcctcccttt ccttggcttt tatcatgcta atatttgcag aaaatattca ataaagtgag     1380

tctttgcact tga                                                        1393


