﻿               SEQUENCE LISTING

<110> Technische Universität München

<120> T cell specific genome editing

<130> TUM161201PCT

<150> EP15197843.4
<151> 2015-12-03

<160> 18

<170> BiSSAP 1.3.6

<210> 1
<211> 11670
<212> DNA
<213> Artificial Sequence


<220> 
<223> circular vector DNA

<220> 
<221> promoter
<222> 4054..4470
<223> /function="CD4 promoter"

<400> 1
gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt       60

cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt      120

tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat      180

aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt      240

ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg      300

ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga      360

tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc      420

tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac      480

actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg      540

gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca      600

acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg      660

gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg      720

acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg      780

gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag      840

ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg      900

gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct      960

cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac     1020

agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact     1080

catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga     1140

tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt     1200

cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct     1260

gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc     1320

taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc     1380

ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc     1440

tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg     1500

ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt     1560

cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg     1620

agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg     1680

gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt     1740

atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag     1800

gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt     1860

gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta     1920

ttaccgcctt tgagtgagct gataccgctc gccgcagccg aaccgaccga gcgcagcgag     1980

tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg     2040

ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc     2100

aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt     2160

ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat     2220

gaccatgatt acgaattgcg gccgctctag aattgaagct tccagctcca ttgtgctgaa     2280

ccaggcagat agaaacagga agtcctgccc ccaaggtgga agaggctgtt aggcccatgt     2340

ctcaacctag agtttggaaa gagaactccc aagcatggac acatttttga ctcgtgggaa     2400

gcagagttct catacatggg cggcaaaaga ctccagagtt agttatcagg cctgtctcct     2460

tgtcatcgtc ttcattttct cagctcagct gagctgggga gcaaaggttg ttgggttcct     2520

ctagcctgcc tcagttcctc ttgttttata gtcttactac gcagccctgg ctggtctgaa     2580

attcacataa agccacctgc ctctgcctag taagtactgg gattaaaggt ctttttccct     2640

ttttatttta agtttcattt tgttggcata gcatctactt tgtagccagg gatgaccctg     2700

aactcatggg cccaagcaag ccagcttcaa ttcaccccac cttccaaaat cttgacttac     2760

acatgccact gttgctgaca ccttggtctc tcttgagttt tccttgctat aagattcctg     2820

cattattcca tctccataga cccttgcctt catttttttc ttaaagtaca gaatcttggg     2880

gaggagggag gttttgaaga caggaaaaca tcctattgtc agggctggaa aggccgttct     2940

gcagttatga gtgcttgttg cctttcaaga agacctaggt ttgatgtcga gcatccagat     3000

ggggcggctc ccaaaacacc tgtaactcca gttccaagga atctgacacc ctcttcaggc     3060

ctctgagcat tcacacaggg tggggaattt atgtatacag acacataaat aaaagtagac     3120

atctaaaaca agtttcccag ccgaatgtgg taggtggtgc atgcctttaa tcccagcact     3180

caggaggcag aggcaggcgc atttctgagt tcaaggccag cctggtctac aaagtgagtt     3240

tcaagacagc cggaatacac agagaaaccc tgtctcgact cgccctcaca cacacacata     3300

aaaccaagtt tctcagtgtc atcttcctag aagatgaaag gccatcccat aatgcaagta     3360

gggttaagga ctagacagct tttgccttca tgcttcctaa tgttggcaag ttatttaatg     3420

cctgtgagcc tcaagtcccg aaatctgtac aaaaaggggc gcgcatcaag tttcacaagt     3480

tgctttacgg aaaatgtata atacagttat atgtagcata gaatgaaggt taacaccgtg     3540

tggctactat acttttccac tgagccctta cccagatccc ccgggctgca ggaattcgat     3600

aattcgattt ggctactgct ccttccacat atgaacttgt ttacagggct tcatggctca     3660

gaacctaccc agagaatttt ctgttctaca tccccaacca agccaaggtg ttggggttca     3720

aatttgagcc ccagctgtta gccctctgca aagaaaaaaa aaaaaaaaaa agaacaaagg     3780

gcctagattt cccttctgag ccccacccta agatgaagcc tcttctttca agggagtggg     3840

gttggggtgg aggcggatcc tgtcagcttt gctctctctg tggctggcag tttctccaaa     3900

gggtaacagg tgtcagctgg ctgagcctag gctgaaccct gagacatgct acctctgtct     3960

tctcatggct ggaggcagcc tttgtaagtc acagaaagta gctgaggggc tctggaaaaa     4020

agacagccag ggtggaggta gattggtccc aattctagaa cagccgactt agtggatctg     4080

ttgggcaacg taatctaaat tttccattga ttaaattgct aacttttttt tttaaaacat     4140

ggcattactg cataggcagc ctagtctggc cttgagcttg tgatttttct gcctaaatca     4200

cccagtgctg gggttacagt tacatgcccg tgcccgtgcc cgtgcctgta agccttgcct     4260

cacgttgacc taaccaggct gtttcagctt tttacggcac caaacaatag tgacattcca     4320

gactccagct tgattctgct caggcatttt cagtgacttt ggcattttta atttttcaac     4380

ttccccaaca actgggggtg ggagggaggg actcctgagg gctggcttac gtccgccgtg     4440

cagaggagcc tcacgaccag gcttcctgtc ttttcattta cgaacatctg tgaaggcaaa     4500

gcaagactct cttcttcact aggtacctgt ttgcaaagtc tcgagccctc atatacacac     4560

acctgtgcaa gaagcagagt gaaggaagga ctggccagag gtaagaggga cgtgttcaac     4620

tttgttcttt caaggagctg gaaggggcag ctaatgtgcc ggaatgttct ggatgcagtt     4680

ctctgttctc agttgtggcc tggggttctc ctgtattcct cttagaacgg gtcatgaaca     4740

gtgatctccc actggaatca tgagcttcga aaggctcagc agtaagccct tctcatcttt     4800

gcctgcccat ggccccaagt atagtttagt gctcagagca gtaggtgccc ccttgaacca     4860

ccccaggaag aatgcaaagt cagaggggta gaaaggcagc tgggacatgg tggggtgtgg     4920

gggcaatagt ggggagtgga aagctgggga gagagaaaat acagaaataa tttagcagag     4980

gactgaagat tagaaagagg aaactttgag aatggaagag acactaaaac aaaacaacaa     5040

caacaaaaag aaacagaaaa cacaagaaaa acagagtgga cgatatgcaa ttaaaaaaaa     5100

aaaactttct taaaagtctt caccaaggat catgcaatca agaacaccaa gcttcaaata     5160

attaacaaaa caacaaaacc ctttcataat attgtctctt acaactagat ttgagtgtat     5220

gtgagtatgt atgtttgaga tagcatattt gtgtgtgagt acatatgtcc agttagcatg     5280

tttgtgtgtg tgtccatgcc agctttttgg gtgtgtgttt gtatccatgc caacttgctc     5340

cagagatcat ttgcatagta aacactttat cttctgacca tctctccagt tcaaaattag     5400

gtatttctta agcaaaggca gcagtctctc tctctctctt ggctttgttg tttgtttgtt     5460

tgattgattg atttttttgt tgtagaactt atgtagaata ggctgacctt gagcatagag     5520

atctacctgg ttttgccttc tgagtgctgg gattaaagat gtatgccacc caagaagaca     5580

ggagtaggtg ggagagcacc ctcatagagg cagtaggagg agatggagta gggggtttct     5640

gaaggggaga cctggaaagg ggaaaacatt tgaaacataa acaatgagaa tagccaataa     5700

aaaataaaaa gaaacagtat atgtatttat aaagaacaca ttagtgtgta tatatataca     5760

tgtataagca tgttaaattt gtgtgtgttt gtaaatctac acatatatgt tccatgaata     5820

taatactctg ctgataaaca tttaatggtt gcctctctaa agcaaaacaa acaaaacacc     5880

cgatggtcaa tatttggcaa cttccatggt gtgaagattc ctgctgaggc tgctttacca     5940

ggacccctcc tggtgacatc actcactgta gagctgggga gaaaactgca taatcaattc     6000

ttgccaatca ctgtggaatg ctgccagtgc agtgacagat aacatacctc ccccaaaaga     6060

tgtgtgccac caccacctgg ctataatttt attaaaaggc aagtgggtcc ttctccccat     6120

acttgagata gggtcttgta taacccaggt tagccttgag cttactatat agctgaggct     6180

ggccttgaac tgggtgtttg cctgcatgta tgtacgcgta ccatgtgatt ggagcctgct     6240

gaggtcagaa gaggacactg gatccacagt aatggaatta cagacatacc catgtaggat     6300

ctcggaatcg aacctgggac ctccacagga ggaacaagtg ctcacaaccc tttagtttta     6360

tttttgagat agggtttcac agtacagtcc ttgctggctt tggactcatg gtcctcctgc     6420

cttggcctcc tagctactaa gagtgtacac atatgcctct gtgccagggt cggagacaat     6480

aacggtgcac gtgaggacct aaaaccgtag tttgtctttg acacccagga atatacttat     6540

ttctgggtct ctgtcatgca ctgctgctat tgctcaggat gtttaaattg ctcttcagct     6600

actgacatca cagtcttagt ttggcaggac ctttggggtg cattactgca gggtgcccac     6660

ttttgtgtat gcagataatg ttctctgggt tggttatcaa ggtcctgagg aagagaaaga     6720

gttcttgtgt gccccaggcc caaggaatac ctgaagactg atgatttcta tcttcctccg     6780

cccctgacat ttttgtaggc tcagattccc aaccaacaag agctcaagga ggccaccatg     6840

gacaagaagt actccattgg gctcgatatc ggcacaaaca gcgtcggctg ggccgtcatt     6900

acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac     6960

agcataaaga agaacctcat tggcgccctc ctgttcgact ccggggagac ggccgaagcc     7020

acgcggctca aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac     7080

ctgcaggaga tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg     7140

gaggagtcct ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat     7200

atcgtggacg aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag     7260

cttgtagaca gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg     7320

atcaaatttc ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc     7380

gacaaactct ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc     7440

aacgcatccg gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg     7500

ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt     7560

atcgccctgt cactcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat     7620

gccaagcttc aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag     7680

atcggcgacc agtacgcaga cctttttttg gcggcaaaga acctgtcaga cgccattctg     7740

ctgagtgata ttctgcgagt gaacacggag atcaccaaag ctccgctgag cgctagtatg     7800

atcaagcgct atgatgagca ccaccaagac ttgactttgc tgaaggccct tgtcagacag     7860

caactgcctg agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga     7920

tacattgacg gcggagcaag ccaggaggaa ttttacaaat ttattaagcc catcttggaa     7980

aaaatggacg gcaccgagga gctgctggta aagcttaaca gagaagatct gttgcgcaaa     8040

cagcgcactt tcgacaatgg aagcatcccc caccagattc acctgggcga actgcacgct     8100

atcctcaggc ggcaagagga tttctacccc tttttgaaag ataacaggga aaagattgag     8160

aaaatcctca catttcggat accctactat gtaggccccc tcgcccgggg aaattccaga     8220

ttcgcgtgga tgactcgcaa atcagaagag accatcactc cctggaactt cgaggaagtc     8280

gtggataagg gggcctctgc ccagtccttc atcgaaagga tgactaactt tgataaaaat     8340

ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt acgagtactt cacagtttat     8400

aacgagctca ccaaggtcaa atacgtcaca gaagggatga gaaagccagc attcctgtct     8460

ggagagcaga agaaagctat cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg     8520

aaacagctca aagaagacta tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc     8580

ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc acgatctcct gaaaatcatt     8640

aaagacaagg acttcctgga caatgaggag aacgaggaca ttcttgagga cattgtcctc     8700

acccttacgt tgtttgaaga tagggagatg attgaagaac gcttgaaaac ttacgctcat     8760

ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc gatatacagg atgggggcgg     8820

ctgtcaagaa aactgatcaa tgggatccga gacaagcaga gtggaaagac aatcctggat     8880

tttcttaagt ccgatggatt tgccaaccgg aacttcatgc agttgatcca tgatgactct     8940

ctcaccttta aggaggacat ccagaaagca caagtttctg gccaggggga cagtcttcac     9000

gagcacatcg ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt     9060

aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc     9120

gagatggccc gagagaacca aactacccag aagggacaga agaacagtag ggaaaggatg     9180

aagaggattg aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt     9240

gaaaacaccc agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac     9300

atgtacgtgg atcaggaact ggacatcaat cggctctccg actacgacgt ggatcatatc     9360

gtgccccagt cttttctcaa agatgattct attgataata aagtgttgac aagatccgat     9420

aaaaatagag ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat     9480

tattggcggc agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact     9540

aaggctgaac gaggtggcct gtctgagttg gataaagccg gcttcatcaa aaggcagctt     9600

gttgagacac gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc     9660

aagtacgatg aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag     9720

ctggtctcag atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac     9780

caccatgcgc atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat     9840

cccaagcttg aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg     9900

atcgcaaagt ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat     9960

attatgaatt ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca    10020

cttatcgaaa caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg    10080

acagtccgga aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag    10140

accggaggct tctccaagga aagtatcctc ccgaaaagga acagcgacaa gctgatcgca    10200

cgcaaaaaag attgggaccc caagaaatac ggcggattcg attctcctac agtcgcttac    10260

agtgtactgg ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag    10320

gaactgctgg gcatcacaat catggagcga tcaagcttcg aaaaaaaccc catcgacttt    10380

ctcgaggcga aaggatataa agaggtcaaa aaagacctca tcattaagct tcccaagtac    10440

tctctctttg agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag    10500

aaaggtaacg agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac    10560

tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa    10620

cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc    10680

ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc    10740

atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct    10800

gcagccttca agtacttcga caccaccata gacagaaagc ggtacacctc tacaaaggag    10860

gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac    10920

ctctctcagc tcggtggaga cagcagggct gaccccaaga agaagaggaa ggtgtgatcg    10980

acgattccat ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa    11040

aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac    11100

ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat    11160

aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat    11220

catgtctgga tcctcacatc ccaatccgcg gctcgaccag cggccgctag cttggcactg    11280

gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt    11340

gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct    11400

tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg    11460

catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc    11520

gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt    11580

ctgctcccgg catccgctac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga    11640

ggttttcacc gtcatcaccg aaacgcgcga                                     11670


<210> 2
<211> 8508
<212> DNA
<213> Artificial Sequence


<220> 
<223> circular vector DNA

<220> 
<221> promoter
<222> 1..251
<223> /function="Human U6 promoter"

<400> 2
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg actaggctgg agaaggaccg ttttagagct agaaatagca agttaaaata      300

aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag      360

ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag      420

acaaatggct ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc      480

gcccaacgac ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg      540

tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat      600

gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca      660

gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat      720

taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc      780

acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg      840

gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg      900

gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag      960

gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgac     1020

gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac     1080

tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt     1140

agctgagcaa gaggtaaggg tttaagggat ggttggttgg tggggtatta atgtttaatt     1200

acctggagca cctgcctgaa atcacttttt ttcaggttgg accggtgcca ccatggacta     1260

taaggaccac gacggagact acaaggatca tgatattgat tacaaagacg atgacgataa     1320

gatggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa     1380

gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga     1440

gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa     1500

gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct     1560

gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga     1620

gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc     1680

cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga     1740

cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga     1800

cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt     1860

ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct     1920

gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag     1980

cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa     2040

tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct     2100

gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact     2160

gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga     2220

ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga     2280

catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag     2340

atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc     2400

tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga     2460

cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga     2520

cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac     2580

cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg     2640

gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct     2700

gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg     2760

gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa     2820

gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa     2880

cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct     2940

gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca     3000

gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct     3060

gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga     3120

agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa     3180

ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac     3240

actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga     3300

cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg     3360

gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa     3420

gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt     3480

taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat     3540

tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt     3600

ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc     3660

cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat     3720

cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac     3780

ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt     3840

ggaccaggaa ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca     3900

gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg     3960

gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg     4020

gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga     4080

gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac     4140

ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga     4200

cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc     4260

cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc     4320

ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct     4380

ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa     4440

gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa     4500

ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga     4560

gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg     4620

gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg     4680

cttcagcaaa gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa     4740

ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct     4800

ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct     4860

ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc     4920

caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt     4980

cgagctggaa aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa     5040

cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa     5100

gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca     5160

ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga     5220

cgctaatctg gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga     5280

gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt     5340

caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga     5400

cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca     5460

gctgggaggc gacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa     5520

gtaagaattc ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct     5580

gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt     5640

tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg     5700

ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg     5760

gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg     5820

ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca     5880

gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg     5940

catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg     6000

gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg     6060

ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc     6120

cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc     6180

tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga     6240

cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa     6300

ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga     6360

tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca     6420

aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat     6480

agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc     6540

tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt     6600

tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat     6660

aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg     6720

tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga     6780

gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac     6840

atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc     6900

cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca     6960

tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc     7020

caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg     7080

ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac     7140

cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca     7200

taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg     7260

agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac     7320

cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg     7380

caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat     7440

taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg     7500

ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc ggtatcattg     7560

cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc     7620

aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc     7680

attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt     7740

tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt     7800

aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt     7860

gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag     7920

cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca     7980

gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca     8040

agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg     8100

ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg     8160

cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct     8220

acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga     8280

gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc     8340

ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg     8400

agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg     8460

cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt                  8508


<210> 3
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> protospacer specific for exon 2 of CD2

<400> 3
gactaggctg gagaaggacc                                                20


<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD4 Cas9 typ fwd primer

<400> 4
tgctcacaac cctttagttt                                                20


<210> 5
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD4 Cas9 typ rev primer

<400> 5
ctttttatcc tcctccacc                                                 19


<210> 6
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> U6 fwd primer

<400> 6
gagggcctat ttcccatgat tcc                                            23


<210> 7
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> T7 gRNA rev primer

<400> 7
gcacgcgcta aaaacgga                                                  18


<210> 8
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> out fwd primer

<400> 8
atcaccctga acatccccaa c                                              21


<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> out rev primer

<400> 9
actggagtct tcttgtgggc                                                20


<210> 10
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> in fwd primer

<400> 10
ctggtcgcag agtttaaaag g                                              21


<210> 11
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> in rev primer

<400> 11
gctgctcccc aactttctac                                                20


<210> 12
<211> 53
<212> DNA
<213> Mus musculus


<220> 
<223> wild type part of exon 2 of CD2

<400> 12
gggatgacta ggctggagaa ggacctggac gtgaggattc tgggtaagtc tcc            53


<210> 13
<211> 55
<212> DNA
<213> Artificial Sequence


<220> 
<223> mutated part of exon 2 of CD2

<400> 13
gggatgacta ggctggagaa ggggacctgg acgtgaggat tctgggtaag tctcc          55


<210> 14
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> mutated part 2 of exon 2 of CD2

<400> 14
gggatgacta ggctggagaa gggggtaagt ctcc                                 34


<210> 15
<211> 54
<212> DNA
<213> Artificial Sequence


<220> 
<223> mutated part 3 of exon 2 of CD2

<400> 15
gggatgacta ggctggagaa ggaacctgga cgtgaggatt ctgggtaagt ctcc           54


<210> 16
<211> 57
<212> DNA
<213> Artificial Sequence


<220> 
<223> mutated part 4 of exon 2 of CD2

<400> 16
gggatgacta ggctggagaa gggtcgacct ggacgtgagg attctgggta agtctcc        57


<210> 17
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> mutated part 5 of exon 2 of CD2

<400> 17
gggatgacta ggctggagag gattctgggt aagtctcc                             38


<210> 18
<211> 54
<212> DNA
<213> Artificial Sequence


<220> 
<223> mutated part 6 of exon 2 of CD2

<400> 18
gggatgacta ggctggagaa gggacctgga cgtgaggatt ctgggtaagt ctcc           54


