                         SEQUENCE LISTING

<110>  LimmaTech Biologics AG
       Eidgenossische Technische Hochschule Zurich
 
<120>  METHODS OF PRODUCING GLYCOSYLATED PROTEINS

<130>  14197-004-228

<140>  TBA
<141>  On even date herewith

<150>  US 62/260,725
<151>  2015-11-30

<150>  US 62/416,853
<151>  2016-11-03

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  620
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ApNGT Sequence

<400>  1

Met Glu Asn Glu Asn Lys Pro Asn Val Ala Asn Phe Glu Ala Ala Val 
1               5                   10                  15      


Ala Val Lys Asp Tyr Glu Lys Ala Cys Ser Glu Leu Leu Leu Ile Leu 
            20                  25                  30          


Ser Gln Leu Asp Ser Asn Phe Gly Gly Ile Gln Glu Ile Glu Phe Glu 
        35                  40                  45              


Tyr Pro Val Gln Leu Gln Asp Leu Glu Gln Glu Lys Ile Val Tyr Phe 
    50                  55                  60                  


Cys Thr Arg Met Ala Thr Ala Ile Thr Thr Leu Phe Ser Asp Pro Val 
65                  70                  75                  80  


Leu Glu Ile Ser Asp Leu Gly Val Gln Arg Phe Leu Val Tyr Gln Arg 
                85                  90                  95      


Trp Leu Ala Leu Ile Phe Ala Ser Ser Pro Phe Val Asn Ala Asp His 
            100                 105                 110         


Ile Leu Gln Thr Tyr Asn Arg Glu Pro Asn Arg Lys Asn Ser Leu Glu 
        115                 120                 125             


Ile His Leu Asp Ser Ser Lys Ser Ser Leu Ile Lys Phe Cys Ile Leu 
    130                 135                 140                 


Tyr Leu Pro Glu Ser Asn Val Asn Leu Asn Leu Asp Val Met Trp Asn 
145                 150                 155                 160 


Ile Ser Pro Glu Leu Cys Ala Ser Leu Cys Phe Ala Leu Gln Ser Pro 
                165                 170                 175     


Arg Phe Ile Gly Thr Ser Thr Ala Phe Asn Lys Arg Ala Thr Ile Leu 
            180                 185                 190         


Gln Trp Phe Pro Arg His Leu Asp Gln Leu Lys Asn Leu Asn Asn Ile 
        195                 200                 205             


Pro Ser Ala Ile Ser His Asp Val Tyr Met His Cys Ser Tyr Asp Thr 
    210                 215                 220                 


Ser Val Asn Lys His Asp Val Lys Arg Ala Leu Asn His Val Ile Arg 
225                 230                 235                 240 


Arg His Ile Glu Ser Glu Tyr Gly Trp Lys Asp Arg Tyr Val Ala His 
                245                 250                 255     


Ile Gly Tyr Arg Asn Asn Lys Pro Val Met Val Val Leu Leu Glu His 
            260                 265                 270         


Phe His Ser Ala His Ser Ile Tyr Arg Thr His Ser Thr Ser Met Ile 
        275                 280                 285             


Ala Ala Arg Glu His Phe Tyr Leu Ile Gly Leu Gly Ser Pro Ser Val 
    290                 295                 300                 


Asp Gln Ala Gly Gln Glu Val Phe Asp Glu Phe His Leu Val Ala Gly 
305                 310                 315                 320 


Asp Asn Met Lys Gln Lys Leu Glu Phe Ile Arg Ser Val Cys Glu Ser 
                325                 330                 335     


Asn Gly Ala Ala Ile Phe Tyr Met Pro Ser Ile Gly Met Asp Met Thr 
            340                 345                 350         


Thr Ile Phe Ala Ser Asn Thr Arg Leu Ala Pro Ile Gln Ala Ile Ala 
        355                 360                 365             


Leu Gly His Pro Ala Thr Thr His Ser Asp Phe Ile Glu Tyr Val Ile 
    370                 375                 380                 


Val Glu Asp Asp Tyr Val Gly Ser Glu Ala Cys Phe Ser Glu Thr Leu 
385                 390                 395                 400 


Leu Arg Leu Pro Lys Asp Ala Leu Pro Tyr Val Pro Ser Ala Leu Ala 
                405                 410                 415     


Pro Glu Lys Val Asp Tyr Leu Leu Arg Glu Asn Pro Glu Val Val Asn 
            420                 425                 430         


Ile Gly Ile Ala Ser Thr Thr Met Lys Leu Asn Pro Tyr Phe Leu Glu 
        435                 440                 445             


Ala Leu Lys Ala Ile Arg Asp Arg Ala Lys Val Lys Val His Phe His 
    450                 455                 460                 


Phe Ala Leu Gly Gln Ser Asn Gly Ile Thr His Pro Tyr Val Glu Arg 
465                 470                 475                 480 


Phe Ile Lys Ser Tyr Leu Gly Asp Ser Ala Thr Ala His Pro His Ser 
                485                 490                 495     


Pro Tyr His Gln Tyr Leu Arg Ile Leu His Asn Cys Asp Met Met Val 
            500                 505                 510         


Asn Pro Phe Pro Phe Gly Asn Thr Asn Gly Ile Ile Asp Met Val Thr 
        515                 520                 525             


Leu Gly Leu Val Gly Val Cys Lys Thr Gly Ala Glu Val His Glu His 
    530                 535                 540                 


Ile Asp Glu Gly Leu Phe Lys Arg Leu Gly Leu Pro Glu Trp Leu Ile 
545                 550                 555                 560 


Ala Asn Thr Val Asp Glu Tyr Val Glu Arg Ala Val Arg Leu Ala Glu 
                565                 570                 575     


Asn His Gln Glu Arg Leu Glu Leu Arg Arg Tyr Ile Ile Glu Asn Asn 
            580                 585                 590         


Gly Leu Asn Thr Leu Phe Thr Gly Asp Pro Arg Pro Met Gly Gln Val 
        595                 600                 605             


Phe Leu Glu Lys Leu Asn Ala Phe Leu Lys Glu Asn 
    610                 615                 620 


<210>  2
<211>  1863
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wild type ApNGT

<400>  2
atggaaaacg aaaataaacc gaatgtagct aattttgaag cggcggttgc ggttaaagat       60

tatgaaaaag cttgctccga attactttta attttgagtc agttagacag taactttggt      120

ggtattcagg agattgagtt tgaatatccg gtgcagcttc aggatttaga acaagaaaaa      180

atagtttatt tttgtacgcg tatggcaacg gcgattacta cgttgttttc cgatcctgtc      240

ttagaaatct ccgatttagg cgttcagaga tttttggttt atcaacgttg gttagcgtta      300

atctttgcca gttcaccgtt tgtgaatgcg gatcatatat tacaaacata taacagagag      360

ccgaatcgta agaatagttt agagattcat ttagattctt caaaatcgtc attaattaaa      420

ttctgtatcc tgtatttacc ggaatctaac gtaaatttga atctggatgt aatgtggaat      480

atttcacctg aattatgcgc ttctttatgt tttgctttgc aatcgcctcg ttttatcggt      540

acatcaactg cgtttaataa acgagcgacc attttgcaat ggtttccacg acatttggat      600

caacttaaaa acctgaataa tattcctagt gccatttcgc atgacgtata tatgcattgt      660

agttatgata cgtcagtaaa taaacatgat gtgaaaaggg cgttaaatca tgttattcgt      720

cgccatatcg aaagtgaata cggttggaaa gatcgatatg tcgctcatat cggttatcgt      780

aataataaac cggttatggt cgtattactg gaacatttcc attcggccca ttctatttac      840

cgtacgcatt ccacatctat gattgcggcg cgtgaacatt tctatttaat cggtttaggt      900

agtccgtcgg ttgatcaagc gggtcaagag gtttttgatg agttccactt ggttgccggc      960

gataatatga agcagaagtt agaatttatc cgctcagttt gtgagagcaa cggtgccgca     1020

atattttata tgccgagtat cggtatggat atgacgacga ttttcgcaag taatacgcgc     1080

cttgctccga tacaagcgat cgcattgggg catccggcaa caacacattc ggacttcatt     1140

gaatatgtga ttgtggaaga cgattatgtc ggctcggaag cgtgttttag tgaaacatta     1200

ttgcgcttac cgaaagacgc attaccttat gttccgtcag cattagcacc tgagaaggtg     1260

gattatttat tacgtgaaaa tccggaagtg gtaaatatcg gtatagcttc aaccacgatg     1320

aagctaaatc cgtatttctt agaagcgtta aaagcgattc gtgatcgtgc caaagtgaaa     1380

gtgcatttcc attttgcatt ggggcaatca aacggtatta ctcacccgta tgtagaacgc     1440

tttattaaat cttatttagg tgattcggcc actgcgcacc ctcattctcc ttatcatcaa     1500

tatctccgta ttttgcataa ttgcgatatg atggtaaacc cgttcccatt cgggaatacg     1560

aacggaatta tcgatatggt cactttaggc ttagttggtg tgtgtaagac aggagccgaa     1620

gttcatgagc atattgatga agggctgttt aaacgtttag gcttacccga gtggctgata     1680

gcaaatacgg tagatgaata tgttgaacgg gcggttcgct tagcggaaaa tcatcaggag     1740

cgtttagagt tacgtcgata tattattgaa aataacggat tgaacacatt gtttaccggg     1800

gatcctagac cgatgggaca agtattttta gaaaaattaa atgcgttcct aaaagaaaat     1860

taa                                                                   1863


<210>  3
<211>  1863
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon Optimized ApNGT

<400>  3
atggagaacg agaataagcc taatgttgca aactttgagg cggcagttgc tgtcaaagac       60

tacgagaagg cttgctccga gctgttgctg atcttgtctc agctggatag caactttggc      120

ggtattcaag aaatcgagtt cgagtacccg gttcaactcc aggacctcga acaagagaaa      180

attgtatact tctgcacccg catggcgacc gcgatcacga ccttgttttc cgaccctgtt      240

ctggagatca gcgacctggg tgttcagcgt ttcctggttt accaacgttg gctcgcattg      300

atctttgcat cgtccccgtt tgttaacgcg gatcatattc tgcaaaccta taaccgcgaa      360

ccgaaccgta aaaactcttt ggaaatccat ctggactcta gcaaatccag cctcatcaaa      420

ttttgcattt tatacttgcc ggagtccaac gtcaacctga acttggacgt gatgtggaac      480

attagcccgg agttgtgcgc cagcctgtgc ttcgcactgc agtcgccgcg cttcatcggc      540

acctctaccg cgttcaacaa gcgtgcgact attttacaat ggtttccacg tcacctggac      600

cagctgaaaa atttgaacaa cattccgagc gcgatttccc acgacgtcta tatgcattgc      660

tcttacgaca cctccgtgaa caagcatgat gtcaaacgtg cgctgaacca tgtgattcgc      720

cgtcatatcg agtccgaata cggctggaaa gaccgttacg tggcgcacat cggctaccgt      780

aacaacaagc cggtgatggt tgttctgctg gagcactttc actctgcaca ctccatctat      840

cgtacccact cgacctccat gattgcggcc cgcgagcact tttacctgat cggtttgggt      900

agcccgagcg ttgatcaagc gggccaagag gtttttgacg agtttcatct cgttgcgggt      960

gataatatga agcagaagct ggagttcatt cgttcggtgt gcgagtctaa cggtgccgcc     1020

atcttctaca tgccgtccat tggcatggat atgaccacca tcttcgcttc gaatactcgc     1080

cttgcgccta tccaggcgat cgcactgggc caccctgcca ccacccacag cgactttatc     1140

gagtacgtca tcgttgagga cgattacgtg ggctccgagg catgctttag cgaaacgttg     1200

ctgagactgc cgaaggacgc gctgccgtat gtcccgtccg ccttggcccc ggagaaagtt     1260

gattatcttt tgcgtgagaa tccagaggtc gttaatattg gcattgcgag caccaccatg     1320

aagctgaacc cttatttctt ggaagcgctg aaggcgatcc gtgatcgtgc taaagtcaaa     1380

gttcacttcc attttgcgct gggccaaagc aacggtatta cccatccata cgttgagcgt     1440

ttcattaagt cctacctggg tgatagcgcg accgctcacc cacattcccc gtatcaccaa     1500

tacttgcgca tcttgcataa ttgcgacatg atggttaacc cgttcccgtt tggtaacacg     1560

aatggcatca ttgatatggt gaccctcggt ctggttggtg tttgcaaaac cggtgcagag     1620

gtccacgaac acatcgacga gggcttgttt aagcgtctgg gcttgccgga atggctgatt     1680

gccaacaccg ttgacgaata tgtggagcgt gcggtgcgtt tggcggaaaa ccaccaggaa     1740

cgcttggagc tgcgccgcta tattatcgag aataatggct tgaatacgct gtttaccggt     1800

gacccgcgtc cgatgggtca ggtttttctg gaaaaattga acgctttctt gaaggagaac     1860

taa                                                                   1863


<210>  4
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tryptic peptide from scAtaC (Fig. 3)

<400>  4

Gly Asn Leu Ser Thr Ala Ala Asp Val Thr Asp Lys 
1               5                   10          


<210>  5
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glyco-Tag-GFP

<400>  5

Lys Thr Ser Ala His Ala Thr Ala Ser Gly Ala His Ala Thr Ala Gly 
1               5                   10                  15      


Ser Ala Asn Ala Thr Ala Trp Ser His Pro Gln Phe Glu Lys 
            20                  25                  30  


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glyco-Loop-GFP

<400>  6

Leu Ser Gly Ser Gly Ala Asn Ala Thr Ala Thr Gly Ser Gly Leu 
1               5                   10                  15  


<210>  7
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tryptic peptide

<400>  7

Asp Gly Pro Val Leu Ser Gly Ser Gly Ala Asn Ala Thr Ala Thr Gly 
1               5                   10                  15      


Ser Gly Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val Leu Ser 
            20                  25                  30          


Lys 
    


