                         SEQUENCE LISTING

<110>  EMBL
       Baden-Wrttemberg Stiftung gGmbH
 
<120>  Means and methods for preparing engineered proteins by genetic 
       code expansion in insect cells

<130>  M/56326

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  569
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNA(Pyl) expression cassette


<220>
<221>  promoter
<222>  (1)..(400)

<220>
<221>  tRNA
<222>  (401)..(469)
<223>  tRNA sequence

<220>
<221>  3'UTR
<222>  (470)..(569)
<223>  Termination signal

<400>  1
ataagttgag ttatggctta aaaaaaaggt tatttttttt ctatttcata ctgttaaaaa       60

tcaacgcaat ttacaatctg ggaaatgaaa tatccaataa ttaagttagg gttacgaagt      120

aattggaata tcgattcaat tgtaatcgat ttacggtaca gagttcatac tatttacgaa      180

aatgctttaa gtatttctat gatgatcgga tgatttattt aattaaaata ataaaatcta      240

ttagaattac agtattcaga gttaaaacta aataattatc tacataatta atataagtcg      300

attcacattt actcatcgat tattatattt ttaatctgtg caactctgac ttgacattga      360

catgcaatca atgacatcga tcggcaccaa gtatatgttt ggaaacctga tcatgtagat      420

cgaatggact ctaaatccgt tcagccgggt tagattcccg gggtttccgt tttttgtaat      480

cgtagataca atgtcgaaga ttctggcccg tttattgtac atgtgtcccg cgttgctgac      540

tccccaaatt ctggtgttag tttaaaacc                                        569


<210>  2
<211>  561
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNA(Pyl) expression cassette


<220>
<221>  promoter
<222>  (1)..(392)

<220>
<221>  tRNA
<222>  (393)..(461)
<223>  tRNA sequence

<220>
<221>  3'UTR
<222>  (462)..(561)
<223>  Termination signal

<400>  2
acttaactac tcaaaaagtg agggccagca gctcgaccaa tgtaaaacct tgcgaggtgc       60

gaggttaccg gggacccaat caaagagtat aataactata gggaaaggcc caaccccccc      120

cccccccact gtatgtaaaa atataagacc tatttctcaa cctataaacc tatgcaataa      180

aacatccact agattagtct agtgactaga ctagaccatt gttagttaac agtagttcgg      240

ctagatggcg ccaaattggt tcttttagtg aacggtagat ggcgctgtac tcaatcttca      300

tacaaatcat gttaaatgta tgggattcta catcgcgcta tcaaagtttt cattgtgttt      360

gtgaagggta caataatttt gccttggcaa gtggaaacct gatcatgtag atcgaatgga      420

ctctaaatcc gttcagccgg gttagattcc cggggtttcc gttttttgaa gagtttcagt      480

ttggtatggt ttttctattt tcaaattggt atgagggagt aagcataatc aaatttaatt      540

tcttttgtta aactttagct t                                                561


<210>  3
<211>  554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNA(Pyl) expression cassette


<220>
<221>  promoter
<222>  (1)..(385)

<220>
<221>  tRNA
<222>  (386)..(454)
<223>  tRNA sequence

<220>
<221>  3'UTR
<222>  (455)..(554)
<223>  Termination signal

<400>  3
agatctacga attgttattt cgactttaat ttttattaaa ctacgtaatt attgttttat       60

ttttcaatga gtttcgtatt acaaattgtt ctaatgttta cctacatgtt taaaagattt      120

cggcactgat caaaatgtat tcatacctta catactaccc aatcaaaggc tttacaagtt      180

actttcggca catcgtctgt caatgccata acttctgcag aaaatgggtc gagtttcggc      240

ctttcgcatc ctttgccttt ctcttgtaaa cagtacttca tggcgcggtt ttcaactata      300

ctgtaaagta attaaagtaa ttacctacat aattgtatga ttggactacc ttgagtgact      360

tggactaaga tcttggacta agatcggaaa cctgatcatg tagatcgaat ggactctaaa      420

tccgttcagc cgggttagat tcccggggtt tccgtttttt ataatattaa taagttatgg      480

aagaacggtg tctcccaata caggctgtct atgcttaacg ggaggctcca atcacaatct      540

tttttgtaca atcc                                                        554


<210>  4
<211>  554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNA(Pyl) expression casette


<220>
<221>  promoter
<222>  (1)..(385)

<220>
<221>  tRNA
<222>  (386)..(454)
<223>  tRNA sequence

<220>
<221>  3'UTR
<222>  (455)..(554)
<223>  Termination signal

<400>  4
ttatgcgagt gaggttaccg gaggttcaat taccccctta cactgtgtgt aaaatagata       60

acctttttct caacctaaac tcaaactcaa atcatttatt gcattcatgt gtacatttag      120

atgatacata attaggagta tacctagtat acctagtata aacacatgaa taatagacta      180

gctagagtct agtagtgtct acaccagact atttttagtt aacagtagtt taactagatg      240

gcgctaaatt agttctttta gtaaacggta gatggcgctg tacttaatcg tcatacaaat      300

catgccgaat gtatgagatt ctacatcgcg ctatcaaagt ttttattgtg tttgtgagcg      360

gtacaataat tttgccatag caagtggaaa cctgatcatg tagatcgaat ggactctaaa      420

tccgttcagc cgggttagat tcccggggtt tccgtttttt gaagagtttc agtttggtat      480

ggtttttcta ttttcaaatt ggtatgagga aataagcata atcaaattta atttcttttg      540

taaaacttta gctt                                                        554


<210>  5
<211>  554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNA(Pyl) expression cassette


<220>
<221>  promoter
<222>  (1)..(385)

<220>
<221>  tRNA
<222>  (386)..(454)
<223>  tRNA sequeence

<220>
<221>  3'UTR
<222>  (455)..(554)
<223>  Termination signal

<400>  5
agaaataaaa ttgaaatatt cgatcaagtt caattttatg tctactgaga tagttgatat       60

agcataccta ccggtaaatt tctacgttaa aaaaaacaaa acagaaaata tgtcattcat      120

tattttcggt atttagtagc ttttaataaa taatttcaac ataaaaatat acaaaaagaa      180

attattcata ttaatttcta attttcaact taaagatccc gtacagtttg acaaccatta      240

aattaactta tttcttaaag tttaccaaca gatggcgttg tactcaaccc acatacaaat      300

tgcgtcaaat gtatgggatt ctacatcgcg ctatgaaagt tttcattgtg tttgtgagcg      360

gtacaataat tttgccttag caagtggaaa cctgatcatg tagatcgaat ggactctaaa      420

tccgttcagc cgggttagat tcccggggtt tccgtttttt gaagaaattt taaataaaaa      480

aaattgtttt attttatttt tttaagtatt ctctattaca taattctata cgtaggtatt      540

tgtcattcta tgcg                                                        554


<210>  6
<211>  554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNA(Pyl) expression cassette


<220>
<221>  promoter
<222>  (1)..(385)

<220>
<221>  tRNA
<222>  (386)..(454)
<223>  tRNA Sequence

<220>
<221>  3'UTR
<222>  (455)..(554)
<223>  Termination signal

<400>  6
ttgaaaatcg ggttaaaata tacaatatca acgacatcta tcgttcatat tcagaaacgg       60

attacgagtt aactagcgcc atctgttgtt gtgtaagtaa caacactgat atacttgtgt      120

ggaatagttc cgacagaatt tgtagatggc gctgtaataa aaatattatt taaaaacatg      180

tatttttcac aattttatat attattgtaa gatatttcgt gatattttat aataaaaaat      240

acattaatag taaatattgt aattaaaaaa aggtttcacc ttatttcatt aaagatttta      300

agaaatataa catgaaactc taaatcgcga tatcaacatt tttgttgttt ggtgcctaat      360

atacaaaaat tcgtgctcga ccaccggaaa cctgatcatg tagatcgaat ggactctaaa      420

tccgttcagc cgggttagat tcccggggtt tccgtttttt gaagagtttc agtacgttta      480

taattttatt attatttatt tatagtaaaa acgtgactaa taaacaaaga cgattgttta      540

tttgtatgca attt                                                        554


<210>  7
<211>  554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNA(Pyl) expression cassette


<220>
<221>  promoter
<222>  (1)..(385)

<220>
<221>  tRNA
<222>  (386)..(454)
<223>  tRNA sequence

<220>
<221>  3'UTR
<222>  (455)..(554)
<223>  Termination signal

<400>  7
tcggttcaaa atatacaata ccaacgacat ctgtagttca tattcagaaa cgtgtcacgg       60

gttaactagc gccatctatt gttgtgtaag taatattgat aaaacgatgc catactgtgc      120

ggaaaagttc cgacagaatt tatagatggc gctgtaataa aaatattatt taagaacatg      180

tatttttcaa aattttatat attattgtaa gatatttcat gatattttat aataaaaaat      240

atgttaatag taaatattgt aattaaaagt gggtttgacc ttatttcatt gaaaatttaa      300

agaaatataa aacaaaactc taaatcgcga tatcaacatt tttgttgttc ggtgcctaat      360

gtactaaaat tcgtgcttta caaccggaaa cctgatcatg tagatcgaat ggactctaaa      420

tccgttcagc cgggttagat tcccggggtt tccgtttttt gaagaatgtc gctaagatag      480

aattttaata attctttatt tttggtaaat ccgtgactaa aaacaaaagt gattgtttat      540

ttttttaact taag                                                        554


<210>  8
<211>  554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tRNA(Pyl) expression cassette


<220>
<221>  promoter
<222>  (1)..(385)

<220>
<221>  tRNA
<222>  (386)..(454)
<223>  tRNA sequence

<220>
<221>  3'UTR
<222>  (455)..(554)
<223>  Termination signal

<400>  8
attgtttatt ttttataaaa gctgatatat aaataaatat taactgataa ataaaaaaat       60

actttcttgg aacaattgaa gggaataatg atgaaaaatt ttgctacgtg taaaaaaagg      120

actttagttc ttttacgttt cgttagatgg cgctttttac aaagtacgac taccaagttt      180

aattttattc attaaaaata gaaaattagt agaatttgta aatttattct acaaaaaaat      240

ataaataaag tctgaaattt tactatacat aatttttcaa tccaaaatca attactatca      300

tccagtaatt tacaaaatct ctgcatcgcg ctagtaaaat ttttatgcta agaatcatgt      360

ataccaaaac ggttattcca caagtggaaa cctgatcatg tagatcgaat ggactctaaa      420

tccgttcagc cgggttagat tcccggggtt tccgtttttt ggacattttc attttggtga      480

atattttaaa aatgctttgt atttcatcac atcttttatt acatttcttt catcacatca      540

cagtgatttt tttt                                                        554


<210>  9
<211>  69
<212>  DNA
<213>  Methanosarcina mazeii


<220>
<221>  tRNA
<222>  (1)..(69)
<223>  tRNA sequence

<400>  9
ggaaacctga tcatgtagat cgaatggact ctaaatccgt tcagccgggt tagattcccg       60

gggtttccg                                                               69


<210>  10
<211>  107
<212>  DNA
<213>  Homo sapiens

<400>  10
gtgctcgctc cggcagcaca tatactaaaa ttggaacgat acagagaaga ttagcatggc       60

ccctgcgcaa ggatgacacg caaattcgtg aagcgttcca tattttt                    107


<210>  11
<211>  107
<212>  DNA
<213>  Drosophila melanogaster

<400>  11
gttcttgctt cggcagaaca tatactaaaa ttggaacgat acagagaaga ttagcatggc       60

ccctgcgcaa ggatgacacg caaaatcgtg aagcgttcca cattttt                    107


<210>  12
<211>  108
<212>  DNA
<213>  Spodoptera frugiperda

<400>  12
gtacttgctt cggcagtaca tatactaaaa ttggaacgat acagagaaga ttagcatggc       60

ccctgcgcaa ggatgacacg caaaatcgtg aagcgttcca catttttt                   108


<210>  13
<211>  108
<212>  DNA
<213>  Bombyx mori

<400>  13
gtacttgctt cggcagtaca tatactaaaa ttggaacgat acagagaaga ttagcatggc       60

ccctgcgcaa ggatgacacg caaaatcgtg aagcgttcca catttttt                   108


<210>  14
<211>  74
<212>  DNA
<213>  Bombyx mori

<400>  14
gtacatatac taaaattgga acgatacaga gacgattagc atggcccctg cgcaaggatg       60

acacgcaaaa tcgt                                                         74


<210>  15
<211>  356
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6 (H.sapiens) promoter tRNApyl 3term

<400>  15
aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac       60

aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa      120

aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt      180

aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat      240

atcttgtgga aaggacgaaa caccggaaac ctgatcatgt agatcgaatg gactctaaat      300

ccgttcagcc gggttagatt cccggggttt ccgtttttcg gggaaatgtg cgcgga          356


<210>  16
<211>  561
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6 (D. melanogaster) promoter-tRNA Pyl-3term

<400>  16
gttcgacttg cagcctgaaa tacggcacga gtaggaaaag ccgagtcaaa tgccgaatgc       60

agagtctcat tacagcacaa tcaactcaag aaaaactcga cactttttta ccatttgcac      120

ttaaatcctt ttttattcgt tatgtatact ttttttggtc cctaaccaaa acaaaaccaa      180

actctcttag tcgtgcctct atatttaaaa ctatcaattt attatagtca ataaatcgaa      240

ctgtgttttc aacaaacgaa caatggacac tttgattcta aaggaaattt tgaaaatctt      300

aagcagaggg ttcttaagac catttgccaa ttcttataat tctcaactgt ctctttcctg      360

atgttgatca tttatatagg tatgttttcc tcaatacttc ggaaacctga tcatgtagat      420

cgaatggact ctaaatccgt tcagccgggt tagattcccg gggtttccgt ttttttgcta      480

acctgtgatt gctcctactc aaatacaaaa acatcaaatt ttctgtcaat aaagcatatt      540

tatttatatt tattttacag g                                                561


<210>  17
<211>  568
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6  (B.mori) promoter-tRNA Pyl-3term

<400>  17
ttaatattaa ataagtacat accttgagaa tttaaaaatc gtcaactata agccatacga       60

atttaagctt ggtacttggc ttatagataa ggacagaata agaattgtta acgtgtaaga      120

caaggtcaga tagtcatagt gattttgtca aagtaataac agatggcgct gtacaaacca      180

taactgtttt catttgtttt tatggatttt attacaaatt ctaaaggttt tattgttatt      240

atttaatttc gttttaatta tattatatat ctttaataga atatgttaag agtttttgct      300

ctttttgaat aatctttgta aagtcgagtg ttgttgtaaa tcacgctttc aatagtttag      360

tttttttagg tatatataca aaatatcgtg ctctacaagt ggaaacctga tcatgtagat      420

cgaatggact ctaaatccgt tcagccgggt tagattcccg gggtttccgt ttttttagct      480

ttaacacttt gaataaatat tactctctta aatatctttg ctacaagttg tacttaattg      540

tatttaattg tacaaaatat attaaaat                                         568


<210>  18
<211>  561
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6 (S.frugiperda) promoter-tRNA Pyl-3term

<400>  18
acttaactac tcaaaaagtg agggccagca gctcgaccaa tgtaaaacct tgcgaggtgc       60

gaggttaccg gggacccaat caaagagtat aataactata gggaaaggcc caaccccccc      120

cccccccact gtatgtaaaa atataagacc tatttctcaa cctataaacc tatgcaataa      180

aacatccact agattagtct agtgactaga ctagaccatt gttagttaac agtagttcgg      240

ctagatggcg ccaaattggt tcttttagtg aacggtagat ggcgctgtac tcaatcttca      300

tacaaatcat gttaaatgta tgggattcta catcgcgcta tcaaagtttt cattgtgttt      360

gtgaagggta caataatttt gccttggcaa gtggaaacct gatcatgtag atcgaatgga      420

ctctaaatcc gttcagccgg gttagattcc cggggtttcc gttttttgaa gagtttcagt      480

ttggtatggt ttttctattt tcaaattggt atgagggagt aagcataatc aaatttaatt      540

tcttttgtta aactttagct t                                                561


<210>  19
<211>  2536
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pIEx-U6(Human)-tRNAPyl-3'term

<400>  19
cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg       60

accatgatta cgaattcaag gtcgggcagg aagagggcct atttcccatg attccttcat      120

atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca      180

caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag      240

ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg      300

atttcttggc tttatatatc ttgtggaaag gacgaaacac cggaaacctg atcatgtaga      360

tcgaatggac tctaaatccg ttcagccggg ttagattccc ggggtttccg ccatttttcg      420

gggaaatgtg cgcggatcta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc      480

cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc      540

tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc      600

gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg      660

gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat      720

ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc      780

acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa      840

ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa      900

aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt      960

gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct     1020

tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat     1080

gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg     1140

cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg     1200

atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt     1260

attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg     1320

ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg     1380

gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg     1440

tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa     1500

aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt     1560

tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt     1620

tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt     1680

ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag     1740

ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta     1800

gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat     1860

aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg     1920

ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg     1980

agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac     2040

aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga     2100

aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt     2160

ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta     2220

cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat     2280

tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg     2340

accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct     2400

ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa     2460

gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct     2520

ttacacttta tgcttc                                                     2536


<210>  20
<211>  3160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pIEx-U6(Dm)-2-tRNAPyl-3term

<400>  20
aattcgcgat cgcaaaaaga tctgttcgac ttgcagcctg aaatacggca cgagtaggaa       60

aagccgagtc aaatgccgaa tgcagagtct cattacagca caatcaactc aagaaaaact      120

cgacactttt ttaccatttg cacttaaatc cttttttatt cgttatgtat actttttttg      180

gtccctaacc aaaacaaaac caaactctct tagtcgtgcc tctatattta aaactatcaa      240

tttattatag tcaataaatc gaactgtgtt ttcaacaaac gaacaatgga cactttgatt      300

ctaaaggaaa ttttgaaaat cttaagcaga gggttcttaa gaccatttgc caattcttat      360

aattctcaac tgtctctttc ctgatgttga tcatttatat aggtatgttt tcctcaatac      420

ttcggaaacc tgatcatgta gatcgaatgg actctaaatc cgttcagccg ggttagattc      480

ccggggtttc cgtttttttg ctaacctgtg attgctccta ctcaaataca aaaacatcaa      540

attttctgtc aataaagcat atttatttat atttatttta caggggatcc acgcgtgcgg      600

ccgcacagct gtatacacgt gcaagccagc cagaactcgc cccggaagac cccgaggatc      660

tcgagcacta agtgattaac ctcaggttat acatatattt tgaatttaat taattataca      720

tatattttat attatttttg tcttttatta tcgaggggcc gttgttggtg tggggttttg      780

catagaaata acaatgggag ttggcgacgt tgctgcgcca acaccacctc ccttccctcc      840

tttcatcatg tatctgtaga taaaataaaa tattaaacct aaaaacaaga ccgcgcctat      900

caacaaaatg ataggcatta acttgccgct gacgctgtca ctaacgttgg acgatttgcc      960

gactaaacct tcatcgccca gtaaccaatc tagacgtcag gtggcacttt tcggggaaat     1020

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg     1080

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa     1140

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac     1200

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac     1260

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt     1320

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc     1380

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca     1440

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc     1500

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1560

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1620

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1680

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1740

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1800

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1860

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1920

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1980

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     2040

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     2100

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     2160

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     2220

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     2280

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     2340

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     2400

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     2460

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     2520

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     2580

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2640

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2700

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2760

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2820

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2880

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata     2940

cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt     3000

cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag     3060

gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga     3120

taacaatttc acacaggaaa cagctatgac catgattacg                           3160


<210>  21
<211>  4715
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pIEx-U6(Dm)-2-tRNAPyl-3'term 4 x

<400>  21
aaataggcgt atcacgaggc cctttcgttc gcgcgtttcg gtgatgacgg tgaaaacctc       60

tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga      120

caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg      180

gcatcagagc agattgtact gagagtgcac catatggcga tcgcgttcga cttgcagcct      240

gaaatacggc acgagtagga aaagccgagt caaatgccga atgcagagtc tcattacagc      300

acaatcaact caagaaaaac tcgacacttt tttaccattt gcacttaaat ccttttttat      360

tcgttatgta tacttttttt ggtccctaac caaaacaaaa ccaaactctc ttagtcgtgc      420

ctctatattt aaaactatca atttattata gtcaataaat cgaactgtgt tttcaacaaa      480

cgaacaatgg acactttgat tctaaaggaa attttgaaaa tcttaagcag agggttctta      540

agaccatttg ccaattctta taattctcaa ctgtctcttt cctgatgttg atcatttata      600

taggtatgtt ttcctcaata cttcggaaac ctgatcatgt agatcgaatg gactctaaat      660

ccgttcagcc gggttagatt cccggggttt ccgttttttt gctaacctgt gattgctcct      720

actcaaatac aaaaacatca aattttctgt caataaagca tatttattta tatttatttt      780

acagggaatt cgttcgactt gcagcctgaa atacggcacg agtaggaaaa gccgagtcaa      840

atgccgaatg cagagtctca ttacagcaca atcaactcaa gaaaaactcg acactttttt      900

accatttgca cttaaatcct tttttattcg ttatgtatac tttttttggt ccctaaccaa      960

aacaaaacca aactctctta gtcgtgcctc tatatttaaa actatcaatt tattatagtc     1020

aataaatcga actgtgtttt caacaaacga acaatggaca ctttgattct aaaggaaatt     1080

ttgaaaatct taagcagagg gttcttaaga ccatttgcca attcttataa ttctcaactg     1140

tctctttcct gatgttgatc atttatatag gtatgttttc ctcaatactt cggaaacctg     1200

atcatgtaga tcgaatggac tctaaatccg ttcagccggg ttagattccc ggggtttccg     1260

tttttttgct aacctgtgat tgctcctact caaatacaaa aacatcaaat tttctgtcaa     1320

taaagcatat ttatttatat ttattttaca ggagatctgt tcgacttgca gcctgaaata     1380

cggcacgagt aggaaaagcc gagtcaaatg ccgaatgcag agtctcatta cagcacaatc     1440

aactcaagaa aaactcgaca cttttttacc atttgcactt aaatcctttt ttattcgtta     1500

tgtatacttt ttttggtccc taaccaaaac aaaaccaaac tctcttagtc gtgcctctat     1560

atttaaaact atcaatttat tatagtcaat aaatcgaact gtgttttcaa caaacgaaca     1620

atggacactt tgattctaaa ggaaattttg aaaatcttaa gcagagggtt cttaagacca     1680

tttgccaatt cttataattc tcaactgtct ctttcctgat gttgatcatt tatataggta     1740

tgttttcctc aatacttcgg aaacctgatc atgtagatcg aatggactct aaatccgttc     1800

agccgggtta gattcccggg gtttccgttt ttttgctaac ctgtgattgc tcctactcaa     1860

atacaaaaac atcaaatttt ctgtcaataa agcatattta tttatattta ttttacaggg     1920

gatccgttcg acttgcagcc tgaaatacgg cacgagtagg aaaagccgag tcaaatgccg     1980

aatgcagagt ctcattacag cacaatcaac tcaagaaaaa ctcgacactt ttttaccatt     2040

tgcacttaaa tcctttttta ttcgttatgt atactttttt tggtccctaa ccaaaacaaa     2100

accaaactct cttagtcgtg cctctatatt taaaactatc aatttattat agtcaataaa     2160

tcgaactgtg ttttcaacaa acgaacaatg gacactttga ttctaaagga aattttgaaa     2220

atcttaagca gagggttctt aagaccattt gccaattctt ataattctca actgtctctt     2280

tcctgatgtt gatcatttat ataggtatgt tttcctcaat acttcggaaa cctgatcatg     2340

tagatcgaat ggactctaaa tccgttcagc cgggttagat tcccggggtt tccgtttttt     2400

tgctaacctg tgattgctcc tactcaaata caaaaacatc aaattttctg tcaataaagc     2460

atatttattt atatttattt tacagggtcg acctgcaggc atgcaagctt ggcgtaatca     2520

tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga     2580

gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt     2640

gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga     2700

atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc     2760

actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg     2820

gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc     2880

cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc     2940

ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga     3000

ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc     3060

ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat     3120

agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg     3180

cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc     3240

aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga     3300

gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact     3360

agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt     3420

ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag     3480

cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg     3540

tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa     3600

aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata     3660

tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg     3720

atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata     3780

cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg     3840

gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct     3900

gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt     3960

tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc     4020

tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga     4080

tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt     4140

aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc     4200

atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa     4260

tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca     4320

catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca     4380

aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct     4440

tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc     4500

gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa     4560

tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt     4620

tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc     4680

taagaaacca ttattatcat gacattaacc tataa                                4715


<210>  22
<211>  2281
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pIDK

<400>  22
gatactagta tacggacctt taattcaacc caacacaata tattatagtt aaataagaat       60

tattatcaaa tcatttgtat attaattaaa atactatact gtaaattaca ttttatttac      120

aatcactcga cgaagacttg atcacccggg atctcgagcc atggtgctag cagctgatgc      180

atagcatgcg gtaccgggag atgggggagg ctaactgaaa cacggaagga gacaataccg      240

gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc      300

gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag      360

accccattgg gaccaatacg cccgcgtttc ttccttttcc ccaccccaac ccccaagttc      420

gggtgaaggc ccagggctcg cagccaacgt cggggcggca agccctgcca tagccactac      480

gggtacgttt aaacccatgt gcctggcaga taacttcgta taatgtatgc tatacgaagt      540

tatggtacgt actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag      600

ctctcatgtt taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt      660

actaagctct catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa      720

tcagcaactt aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag      780

gcttttaaag cttttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg      840

agaagccctt agagcctctc aaagcaattt tcagtgacac aggaacactt aacggctgac      900

agaattagct tcacgctgcc gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa      960

cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc     1020

tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac     1080

atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg     1140

ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc     1200

aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg     1260

catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt     1320

cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc     1380

agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact     1440

gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt     1500

gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca     1560

ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat     1620

gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg     1680

catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga     1740

agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga     1800

cggcgaggat ctcgtcgtga cacatggcga tgcctgcttg ccgaatatca tggtggaaaa     1860

tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga     1920

catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt     1980

cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct     2040

tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac     2100

ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc     2160

gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc     2220

gcccaccccg ggatctatgt cgggtgcgga gaaagaggta atgaaatggc acctaggtat     2280

c                                                                     2281


<210>  23
<211>  2700
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pIDK-U6(Bm)-2-tRNAPyl-3'term

<400>  23
ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat       60

ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg      120

atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg      180

ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc      240

cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg      300

tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt      360

tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg      420

cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat      480

catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca      540

ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca      600

ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa      660

ggcgcgcatg cccgacggcg aggatctcgt cgtgacacat ggcgatgcct gcttgccgaa      720

tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc      780

ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga      840

atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc      900

cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac      960

caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg     1020

ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc     1080

atgctggagt tcttcgccca ccccgggatc tatgtcgggt gcggagaaag aggtaatgaa     1140

atggcaccta ggtatcgata ctagtttaat attaaataag tacatacctt gagaatttaa     1200

aaatcgtcaa ctataagcca tacgaattta agcttggtac ttggcttata gataaggaca     1260

gaataagaat tgttaacgtg taagacaagg tcagatagtc atagtgattt tgtcaaagta     1320

ataacagatg gcgctgtaca aaccataact gttttcattt gtttttatgg attttattac     1380

aaattctaaa ggttttattg ttattattta atttcgtttt aattatatta tatatcttta     1440

atagaatatg ttaagagttt ttgctctttt tgaataatct ttgtaaagtc gagtgttgtt     1500

gtaaatcacg ctttcaatag tttagttttt ttaggtatat atacaaaata tcgtgctcta     1560

caagtggaaa cctgatcatg tagatcgaat ggactctaaa tccgttcagc cgggttagat     1620

tcccggggtt tccgtttttt tagctttaac actttgaata aatattactc tcttaaatat     1680

ctttgctaca agttgtactt aattgtattt aattgtacaa aatatattaa aatccatggt     1740

gctagcagct gatgcatagc atgcggtacc gggagatggg ggaggctaac tgaaacacgg     1800

aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg     1860

cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt     1920

cgatacccca ccgagacccc attgggacca atacgcccgc gtttcttcct tttccccacc     1980

ccaaccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaagccc     2040

tgccatagcc actacgggta cgtttaaacc catgtgcctg gcagataact tcgtataatg     2100

tatgctatac gaagttatgg tacgtactaa gctctcatgt ttcacgtact aagctctcat     2160

gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa cgtactaagc     2220

tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat gtttgaacaa     2280

taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt tataagaaaa     2340

aaaagaatat ataaggcttt taaagctttt aaggtttaac ggttgtggac aacaagccag     2400

ggatgtaacg cactgagaag cccttagagc ctctcaaagc aattttcagt gacacaggaa     2460

cacttaacgg ctgacagaat tagcttcacg ctgccgcaag cactcagggc gcaagggctg     2520

ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa     2580

tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc     2640

ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc     2700


<210>  24
<211>  3130
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pIEx-U6(Sf21)-2-tRNAPyl-3'term

<400>  24
aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta       60

atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta      120

tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt      180

acgaattcac ttaactactc aaaaagtgag ggccagcagc tcgaccaatg taaaaccttg      240

cgaggtgcga ggttaccggg gacccaatca aagagtataa taactatagg gaaaggccca      300

accccccccc cccccactgt atgtaaaaat ataagaccta tttctcaacc tataaaccta      360

tgcaataaaa catccactag attagtctag tgactagact agaccattgt tagttaacag      420

tagttcggct agatggcgcc aaattggttc ttttagtgaa cggtagatgg cgctgtactc      480

aatcttcata caaatcatgt taaatgtatg ggattctaca tcgcgctatc aaagttttca      540

ttgtgtttgt gaagggtaca ataattttgc cttggcaagt ggaaacctga tcatgtagat      600

cgaatggact ctaaatccgt tcagccgggt tagattcccg gggtttccgt tttttgaaga      660

gtttcagttt ggtatggttt ttctattttc aaattggtat gagggagtaa gcataatcaa      720

atttaatttc ttttgttaaa ctttagcttg cggccgcaca gctgtataca cgtgcaagcc      780

agccagaact cgccccggaa gaccccgagg atctcgagca ctaagtgatt aacctcaggt      840

tatacatata ttttgaattt aattaattat acatatattt tatattattt ttgtctttta      900

ttatcgaggg gccgttgttg gtgtggggtt ttgcatagaa ataacaatgg gagttggcga      960

cgttgctgcg ccaacaccac ctcccttccc tcctttcatc atgtatctgt agataaaata     1020

aaatattaaa cctaaaaaca agaccgcgcc tatcaacaaa atgataggca ttaacttgcc     1080

gctgacgctg tcactaacgt tggacgattt gccgactaaa ccttcatcgc ccagtaacca     1140

atctagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt     1200

ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa     1260

taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt     1320

tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat     1380

gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag     1440

atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg     1500

ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata     1560

cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat     1620

ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc     1680

aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg     1740

ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac     1800

gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact     1860

ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa     1920

gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct     1980

ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc     2040

tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga     2100

cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac     2160

tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag     2220

atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg     2280

tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc     2340

tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag     2400

ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc     2460

cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac     2520

ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc     2580

gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt     2640

tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt     2700

gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc     2760

ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt     2820

tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca     2880

ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt     2940

tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt     3000

attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag     3060

tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg     3120

ccgattcatt                                                            3130


<210>  25
<211>  6145
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pIZT-PylRS-mCherry-GFP(Y39TAG):

<400>  25
aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgatga       60

taaacaatgt atggtgctaa tgttgcttca acaacaattc tgttgaactg tgttttcatg      120

tttgccaaca agcaccttta tactcggtgg cctccccacc accaactttt ttgcactgca      180

aaaaaacacg cttttgcacg cgggcccata catagtacaa actctacgtt tcgtagacta      240

ttttacataa atagtctaca ccgttgtata cgctccaaat acactaccac acattgaacc      300

tttttgcagt gcaaaaaagt acgtgtcggc agtcacgtag gccggcctta tcgggtcgcg      360

tcctgtcacg tacgaatcac attatcggac cggacgagtg ttgtcttatc gtgacaggac      420

gccagcttcc tgtgttgcta accgcagccg gacgcaactc cttatcggaa caggacgcgc      480

ctccatatca gccgcgcgtt atctcatgcg cgtgaccgga cacgaggcgc ccgtcccgct      540

tatcgcgcct ataaatacag cccgcaacga tctggtaaac acagttgaac agcatctgtt      600

cgaatttaaa gcttggtacc atggacaaaa aaccgctgaa taccctgatc tctgctactg      660

gtctgtggat gagtcgtacc ggaaccattc ataaaatcaa acaccacgag gttagccgtt      720

cgaaaatcta tattgagatg gcgtgtggcg atcatctggt tgtgaacaat agccgctctt      780

ctcgtacagc acgtgcactg cgtcaccaca aatatcgtaa aacctgtaaa cgttgccgtg      840

tgtccgatga ggatctgaac aaattcctga caaaagccaa tgaggaccaa acaagcgtga      900

aagtgaaagt cgttagcgct cctacccgta ctaaaaaagc aatgccgaaa tccgttgctc      960

gtgcccctaa accactggaa aacactgaag cagcacaggc acagccgtct ggaagcaaat     1020

tctctccggc cattcctgtt tctacccagg agtccgtttc tgttccagca agtgtgagca     1080

ccagcattag cagtattagc accggtgcca ccgctagcgc cctggttaaa ggcaatacca     1140

atccgattac aagcatgtct gccccggttc aagcatcagc tccagcactg acaaaatccc     1200

aaaccgatcg tctggaggtt ctgctgaatc cgaaagacga aatcagcctg aattccggca     1260

aaccgtttcg tgaactggag agcgaactgc tgtcacgtcg taaaaaagac ctgcaacaaa     1320

tctatgccga agaacgtgag aactatctgg ggaaactgga acgtgaaatc acccgctttt     1380

tcgtggatcg tggctttctg gagatcaaat ccccgattct gattcctctg gagtatatcg     1440

agcgtatggg catcgacaat gataccgaac tgagcaaaca aattttccgt gtggataaaa     1500

acttctgtct gcgccctatg ctggcaccaa atctgtataa ctatctgcgc aaactggacc     1560

gtgccctgcc tgatcctatc aaaatcttcg agatcggccc gtgttatcgt aaagagtccg     1620

acggtaaaga acatctggag gagtttacca tgctgaactt ttgccaaatg ggttcaggtt     1680

gtactcgtga gaacctggaa agcatcatca ccgattttct gaaccacctg ggcattgact     1740

tcaaaattgt gggcgacagc tgtatggtgt atggcgacac cctggatgtc atgcacggcg     1800

acctggaact gtctagtgcc gttgttggac caattccgct ggaccgtgag tggggtatcg     1860

acaaaccgtg gatcggagca ggattcggtc tggaacgcct gctgaaagtg aaacacgact     1920

tcaaaaacat caaacgtgcc gcccgttctg aatcgtatta taacgggatc tctacgaacc     1980

tgtaatctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc     2040

gattctacgc gtaccggtca tcatcaccat caccattgag tttatctgac taaatcttag     2100

tttgtattgt catgttttaa tacaatatgt tatgtttaaa tatgttttta ataaatttta     2160

taaaataatt tcaactttta ttgtaacaac attgtccatt tacacactcc tttcaagcgc     2220

gtgggatcga tgctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg     2280

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt     2340

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa     2400

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct     2460

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc     2520

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg     2580

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct     2640

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag     2700

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga     2760

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga     2820

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg     2880

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag     2940

aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag     3000

ggattttggt catgcgaaac acgcacggcg cgcgcacgca gcttagcaca aacgcgtcgt     3060

tgcacgcgcc caccgctaac cgcaggccaa tcggtcggcc ggcctcatat ccgctcacca     3120

gccgcgtcct atcgggcgcg gcttccgcgc ccattttgaa taaataaacg ataacgccgt     3180

tggtggcgtg aggcatgtaa aaggttacat cattatcttg ttcgccatcc ggttggtata     3240

aatagacgtt catgttggtt tttgtttcag ttgcaagttg gctgcggcgc gcgcagcacc     3300

tttgccggga tctgccgggc tgcagcacgt gttgacaatt aatcatcggc atagtatatc     3360

ggcatagtat aatacgacaa ggtgaggaac taaaccatgg acggccgcct ggaaagcacc     3420

ccgccgaaaa aaaaacgcaa agtggaagat agcgcgagcg attacaaaga tgatgatgat     3480

aaagtggcgt acgcggtgag caagggcgag gaggataaca tggccatcat caaggagttc     3540

atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc     3600

gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt     3660

ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc     3720

tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc     3780

aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc     3840

tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcacctcc     3900

gacggccccg taatgcagaa gaagacgatg ggctgggagg cctcctccga gcggatgtac     3960

accgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc     4020

cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc     4080

gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg     4140

gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag     4200

accctgcagg aattcccccc tcccccagcg agcgattaca aagatgatga tgataaagtg     4260

agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac     4320

gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctatggcaag     4380

ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg     4440

accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac     4500

gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag     4560

gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac     4620

cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg     4680

gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc     4740

aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac     4800

taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg     4860

agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg     4920

gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gcatcaccat     4980

caccatcact gaccgacgcc gaccaacacc gccggtccga cgcggcccga cgggtccgag     5040

gggggtcgac ctcgaaactt gtttattgca gcttataatg gttacaaata aagcaatagc     5100

atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa     5160

ctcatcaatg tatcttatca tgtctggatc atgagacaat aaccctgata aatgcttcaa     5220

taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt     5280

tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat     5340

gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag     5400

atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg     5460

ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata     5520

cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat     5580

ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc     5640

aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg     5700

ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac     5760

gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact     5820

ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa     5880

gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct     5940

ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc     6000

tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga     6060

cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac     6120

tcatatatac tttagattga tttaa                                           6145


<210>  26
<211>  1365
<212>  DNA
<213>  Methanosarcina mazeii


<220>
<221>  CDS
<222>  (1)..(1365)

<400>  26
atg gac aaa aaa ccg ctg aat acc ctg atc tct gct act ggt ctg tgg         48
Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp           
1               5                   10                  15                

atg agt cgt acc gga acc att cat aaa atc aaa cac cac gag gtt agc         96
Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser           
            20                  25                  30                    

cgt tcg aaa atc tat att gag atg gcg tgt ggc gat cat ctg gtt gtg        144
Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val           
        35                  40                  45                        

aac aat agc cgc tct tct cgt aca gca cgt gca ctg cgt cac cac aaa        192
Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys           
    50                  55                  60                            

tat cgt aaa acc tgt aaa cgt tgc cgt gtg tcc gat gag gat ctg aac        240
Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn           
65                  70                  75                  80            

aaa ttc ctg aca aaa gcc aat gag gac caa aca agc gtg aaa gtg aaa        288
Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys           
                85                  90                  95                

gtc gtt agc gct cct acc cgt act aaa aaa gca atg ccg aaa tcc gtt        336
Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val           
            100                 105                 110                   

gct cgt gcc cct aaa cca ctg gaa aac act gaa gca gca cag gca cag        384
Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln           
        115                 120                 125                       

ccg tct gga agc aaa ttc tct ccg gcc att cct gtt tct acc cag gag        432
Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu           
    130                 135                 140                           

tcc gtt tct gtt cca gca agt gtg agc acc agc att agc agt att agc        480
Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser           
145                 150                 155                 160           

acc ggt gcc acc gct agc gcc ctg gtt aaa ggc aat acc aat ccg att        528
Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile           
                165                 170                 175               

aca agc atg tct gcc ccg gtt caa gca tca gct cca gca ctg aca aaa        576
Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys           
            180                 185                 190                   

tcc caa acc gat cgt ctg gag gtt ctg ctg aat ccg aaa gac gaa atc        624
Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile           
        195                 200                 205                       

agc ctg aat tcc ggc aaa ccg ttt cgt gaa ctg gag agc gaa ctg ctg        672
Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu           
    210                 215                 220                           

tca cgt cgt aaa aaa gac ctg caa caa atc tat gcc gaa gaa cgt gag        720
Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu           
225                 230                 235                 240           

aac tat ctg ggg aaa ctg gaa cgt gaa atc acc cgc ttt ttc gtg gat        768
Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp           
                245                 250                 255               

cgt ggc ttt ctg gag atc aaa tcc ccg att ctg att cct ctg gag tat        816
Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr           
            260                 265                 270                   

atc gag cgt atg ggc atc gac aat gat acc gaa ctg agc aaa caa att        864
Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile           
        275                 280                 285                       

ttc cgt gtg gat aaa aac ttc tgt ctg cgc cct atg ctg gca cca aat        912
Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro Asn           
    290                 295                 300                           

ctg tat aac tat ctg cgc aaa ctg gac cgt gcc ctg cct gat cct atc        960
Leu Tyr Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile           
305                 310                 315                 320           

aaa atc ttc gag atc ggc ccg tgt tat cgt aaa gag tcc gac ggt aaa       1008
Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys           
                325                 330                 335               

gaa cat ctg gag gag ttt acc atg ctg aac ttt tgc caa atg ggt tca       1056
Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly Ser           
            340                 345                 350                   

ggt tgt act cgt gag aac ctg gaa agc atc atc acc gat ttt ctg aac       1104
Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn           
        355                 360                 365                       

cac ctg ggc att gac ttc aaa att gtg ggc gac agc tgt atg gtg tat       1152
His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Tyr           
    370                 375                 380                           

ggc gac acc ctg gat gtc atg cac ggc gac ctg gaa ctg tct agt gcc       1200
Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala           
385                 390                 395                 400           

gtt gtt gga cca att ccg ctg gac cgt gag tgg ggt atc gac aaa ccg       1248
Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro           
                405                 410                 415               

tgg atc gga gca gga ttc ggt ctg gaa cgc ctg ctg aaa gtg aaa cac       1296
Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His           
            420                 425                 430                   

gac ttc aaa aac atc aaa cgt gcc gcc cgt tct gaa tcg tat tat aac       1344
Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn           
        435                 440                 445                       

ggg atc tct acg aac ctg taa                                           1365
Gly Ile Ser Thr Asn Leu                                                   
    450                                                                   


<210>  27
<211>  454
<212>  PRT
<213>  Methanosarcina mazeii

<400>  27

Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  


Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val 
            100                 105                 110         


Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln 
        115                 120                 125             


Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu 
    130                 135                 140                 


Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser 
145                 150                 155                 160 


Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile 
                165                 170                 175     


Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys 
            180                 185                 190         


Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile 
        195                 200                 205             


Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu 
    210                 215                 220                 


Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu 
225                 230                 235                 240 


Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp 
                245                 250                 255     


Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr 
            260                 265                 270         


Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile 
        275                 280                 285             


Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro Asn 
    290                 295                 300                 


Leu Tyr Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile 
305                 310                 315                 320 


Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys 
                325                 330                 335     


Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly Ser 
            340                 345                 350         


Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn 
        355                 360                 365             


His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Tyr 
    370                 375                 380                 


Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala 
385                 390                 395                 400 


Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro 
                405                 410                 415     


Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His 
            420                 425                 430         


Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn 
        435                 440                 445             


Gly Ile Ser Thr Asn Leu 
    450                 


<210>  28
<211>  6145
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pIZT-PylRS-mCherry-GFP(WT):

<400>  28
aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgatga       60

taaacaatgt atggtgctaa tgttgcttca acaacaattc tgttgaactg tgttttcatg      120

tttgccaaca agcaccttta tactcggtgg cctccccacc accaactttt ttgcactgca      180

aaaaaacacg cttttgcacg cgggcccata catagtacaa actctacgtt tcgtagacta      240

ttttacataa atagtctaca ccgttgtata cgctccaaat acactaccac acattgaacc      300

tttttgcagt gcaaaaaagt acgtgtcggc agtcacgtag gccggcctta tcgggtcgcg      360

tcctgtcacg tacgaatcac attatcggac cggacgagtg ttgtcttatc gtgacaggac      420

gccagcttcc tgtgttgcta accgcagccg gacgcaactc cttatcggaa caggacgcgc      480

ctccatatca gccgcgcgtt atctcatgcg cgtgaccgga cacgaggcgc ccgtcccgct      540

tatcgcgcct ataaatacag cccgcaacga tctggtaaac acagttgaac agcatctgtt      600

cgaatttaaa gcttggtacc atggacaaaa aaccgctgaa taccctgatc tctgctactg      660

gtctgtggat gagtcgtacc ggaaccattc ataaaatcaa acaccacgag gttagccgtt      720

cgaaaatcta tattgagatg gcgtgtggcg atcatctggt tgtgaacaat agccgctctt      780

ctcgtacagc acgtgcactg cgtcaccaca aatatcgtaa aacctgtaaa cgttgccgtg      840

tgtccgatga ggatctgaac aaattcctga caaaagccaa tgaggaccaa acaagcgtga      900

aagtgaaagt cgttagcgct cctacccgta ctaaaaaagc aatgccgaaa tccgttgctc      960

gtgcccctaa accactggaa aacactgaag cagcacaggc acagccgtct ggaagcaaat     1020

tctctccggc cattcctgtt tctacccagg agtccgtttc tgttccagca agtgtgagca     1080

ccagcattag cagtattagc accggtgcca ccgctagcgc cctggttaaa ggcaatacca     1140

atccgattac aagcatgtct gccccggttc aagcatcagc tccagcactg acaaaatccc     1200

aaaccgatcg tctggaggtt ctgctgaatc cgaaagacga aatcagcctg aattccggca     1260

aaccgtttcg tgaactggag agcgaactgc tgtcacgtcg taaaaaagac ctgcaacaaa     1320

tctatgccga agaacgtgag aactatctgg ggaaactgga acgtgaaatc acccgctttt     1380

tcgtggatcg tggctttctg gagatcaaat ccccgattct gattcctctg gagtatatcg     1440

agcgtatggg catcgacaat gataccgaac tgagcaaaca aattttccgt gtggataaaa     1500

acttctgtct gcgccctatg ctggcaccaa atctgtataa ctatctgcgc aaactggacc     1560

gtgccctgcc tgatcctatc aaaatcttcg agatcggccc gtgttatcgt aaagagtccg     1620

acggtaaaga acatctggag gagtttacca tgctgaactt ttgccaaatg ggttcaggtt     1680

gtactcgtga gaacctggaa agcatcatca ccgattttct gaaccacctg ggcattgact     1740

tcaaaattgt gggcgacagc tgtatggtgt atggcgacac cctggatgtc atgcacggcg     1800

acctggaact gtctagtgcc gttgttggac caattccgct ggaccgtgag tggggtatcg     1860

acaaaccgtg gatcggagca ggattcggtc tggaacgcct gctgaaagtg aaacacgact     1920

tcaaaaacat caaacgtgcc gcccgttctg aatcgtatta taacgggatc tctacgaacc     1980

tgtaatctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc     2040

gattctacgc gtaccggtca tcatcaccat caccattgag tttatctgac taaatcttag     2100

tttgtattgt catgttttaa tacaatatgt tatgtttaaa tatgttttta ataaatttta     2160

taaaataatt tcaactttta ttgtaacaac attgtccatt tacacactcc tttcaagcgc     2220

gtgggatcga tgctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg     2280

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt     2340

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa     2400

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct     2460

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc     2520

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg     2580

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct     2640

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag     2700

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga     2760

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga     2820

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg     2880

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag     2940

aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag     3000

ggattttggt catgcgaaac acgcacggcg cgcgcacgca gcttagcaca aacgcgtcgt     3060

tgcacgcgcc caccgctaac cgcaggccaa tcggtcggcc ggcctcatat ccgctcacca     3120

gccgcgtcct atcgggcgcg gcttccgcgc ccattttgaa taaataaacg ataacgccgt     3180

tggtggcgtg aggcatgtaa aaggttacat cattatcttg ttcgccatcc ggttggtata     3240

aatagacgtt catgttggtt tttgtttcag ttgcaagttg gctgcggcgc gcgcagcacc     3300

tttgccggga tctgccgggc tgcagcacgt gttgacaatt aatcatcggc atagtatatc     3360

ggcatagtat aatacgacaa ggtgaggaac taaaccatgg acggccgcct ggaaagcacc     3420

ccgccgaaaa aaaaacgcaa agtggaagat agcgcgagcg attacaaaga tgatgatgat     3480

aaagtggcgt acgcggtgag caagggcgag gaggataaca tggccatcat caaggagttc     3540

atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc     3600

gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt     3660

ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc     3720

tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc     3780

aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc     3840

tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcacctcc     3900

gacggccccg taatgcagaa gaagacgatg ggctgggagg cctcctccga gcggatgtac     3960

accgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc     4020

cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc     4080

gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg     4140

gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag     4200

accctgcagg aattcccccc tcccccagcg agcgattaca aagatgatga tgataaagtg     4260

agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac     4320

gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctatggcaag     4380

ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg     4440

accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac     4500

gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag     4560

gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac     4620

cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg     4680

gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc     4740

aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac     4800

taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg     4860

agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg     4920

gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gcatcaccat     4980

caccatcact gaccgacgcc gaccaacacc gccggtccga cgcggcccga cgggtccgag     5040

gggggtcgac ctcgaaactt gtttattgca gcttataatg gttacaaata aagcaatagc     5100

atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa     5160

ctcatcaatg tatcttatca tgtctggatc atgagacaat aaccctgata aatgcttcaa     5220

taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt     5280

tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat     5340

gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag     5400

atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg     5460

ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata     5520

cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat     5580

ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc     5640

aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg     5700

ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac     5760

gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact     5820

ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa     5880

gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct     5940

ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc     6000

tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga     6060

cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac     6120

tcatatatac tttagattga tttaa                                           6145


<210>  29
<211>  1365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant PylRS AF:


<220>
<221>  CDS
<222>  (1)..(1365)

<400>  29
atg gac aaa aaa ccg ctg aat acc ctg atc tct gct act ggt ctg tgg         48
Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp           
1               5                   10                  15                

atg agt cgt acc gga acc att cat aaa atc aaa cac cac gag gtt agc         96
Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser           
            20                  25                  30                    

cgt tcg aaa atc tat att gag atg gcg tgt ggc gat cat ctg gtt gtg        144
Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val           
        35                  40                  45                        

aac aat agc cgc tct tct cgt aca gca cgt gca ctg cgt cac cac aaa        192
Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys           
    50                  55                  60                            

tat cgt aaa acc tgt aaa cgt tgc cgt gtg tcc gat gag gat ctg aac        240
Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn           
65                  70                  75                  80            

aaa ttc ctg aca aaa gcc aat gag gac caa aca agc gtg aaa gtg aaa        288
Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys           
                85                  90                  95                

gtc gtt agc gct cct acc cgt act aaa aaa gca atg ccg aaa tcc gtt        336
Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val           
            100                 105                 110                   

gct cgt gcc cct aaa cca ctg gaa aac act gaa gca gca cag gca cag        384
Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln           
        115                 120                 125                       

ccg tct gga agc aaa ttc tct ccg gcc att cct gtt tct acc cag gag        432
Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu           
    130                 135                 140                           

tcc gtt tct gtt cca gca agt gtg agc acc agc att agc agt att agc        480
Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser           
145                 150                 155                 160           

acc ggt gcc acc gct agc gcc ctg gtt aaa ggc aat acc aat ccg att        528
Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile           
                165                 170                 175               

aca agc atg tct gcc ccg gtt caa gca tca gct cca gca ctg aca aaa        576
Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys           
            180                 185                 190                   

tcc caa acc gat cgt ctg gag gtt ctg ctg aat ccg aaa gac gaa atc        624
Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile           
        195                 200                 205                       

agc ctg aat tcc ggc aaa ccg ttt cgt gaa ctg gag agc gaa ctg ctg        672
Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu           
    210                 215                 220                           

tca cgt cgt aaa aaa gac ctg caa caa atc tat gcc gaa gaa cgt gag        720
Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu           
225                 230                 235                 240           

aac tat ctg ggg aaa ctg gaa cgt gaa atc acc cgc ttt ttc gtg gat        768
Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp           
                245                 250                 255               

cgt ggc ttt ctg gag atc aaa tcc ccg att ctg att cct ctg gag tat        816
Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr           
            260                 265                 270                   

atc gag cgt atg ggc atc gac aat gat acc gaa ctg agc aaa caa att        864
Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile           
        275                 280                 285                       

ttc cgt gtg gat aaa aac ttc tgt ctg cgc cct atg ctg gca cca aat        912
Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro Asn           
    290                 295                 300                           

ctg gct aac tat ctg cgc aaa ctg gac cgt gcc ctg cct gat cct atc        960
Leu Ala Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile           
305                 310                 315                 320           

aaa atc ttc gag atc ggc ccg tgt tat cgt aaa gag tcc gac ggt aaa       1008
Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys           
                325                 330                 335               

gaa cat ctg gag gag ttt acc atg ctg aac ttt tgc caa atg ggt tca       1056
Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly Ser           
            340                 345                 350                   

ggt tgt act cgt gag aac ctg gaa agc atc atc acc gat ttt ctg aac       1104
Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn           
        355                 360                 365                       

cac ctg ggc att gac ttc aaa att gtg ggc gac agc tgt atg gtg ttt       1152
His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Phe           
    370                 375                 380                           

ggc gac acc ctg gat gtc atg cac ggc gac ctg gaa ctg tct agt gcc       1200
Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala           
385                 390                 395                 400           

gtt gtt gga cca att ccg ctg gac cgt gag tgg ggt atc gac aaa ccg       1248
Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro           
                405                 410                 415               

tgg atc gga gca gga ttc ggt ctg gaa cgc ctg ctg aaa gtg aaa cac       1296
Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His           
            420                 425                 430                   

gac ttc aaa aac atc aaa cgt gcc gcc cgt tct gaa tcg tat tat aac       1344
Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn           
        435                 440                 445                       

ggg atc tct acg aac ctg taa                                           1365
Gly Ile Ser Thr Asn Leu                                                   
    450                                                                   


<210>  30
<211>  454
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  30

Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  


Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val 
            100                 105                 110         


Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln 
        115                 120                 125             


Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu 
    130                 135                 140                 


Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser 
145                 150                 155                 160 


Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile 
                165                 170                 175     


Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys 
            180                 185                 190         


Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile 
        195                 200                 205             


Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu 
    210                 215                 220                 


Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu 
225                 230                 235                 240 


Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp 
                245                 250                 255     


Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr 
            260                 265                 270         


Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile 
        275                 280                 285             


Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro Asn 
    290                 295                 300                 


Leu Ala Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile 
305                 310                 315                 320 


Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys 
                325                 330                 335     


Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly Ser 
            340                 345                 350         


Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn 
        355                 360                 365             


His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Phe 
    370                 375                 380                 


Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala 
385                 390                 395                 400 


Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro 
                405                 410                 415     


Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His 
            420                 425                 430         


Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn 
        435                 440                 445             


Gly Ile Ser Thr Asn Leu 
    450                 


<210>  31
<211>  4733
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBAD-GFP(Y39TAG)-6His:

<400>  31
aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga ccaaagccat       60

gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca ttgattattt      120

gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag cggatcctac      180

ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttg ggctaacagg      240

aggaattaac catggattac aaagatgatg atgataaagt gagcaagggc gaggagctgt      300

tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca      360

gcgtgtccgg cgagggcgag ggcgatgcca cctagggcaa gctgaccctg aagttcatct      420

gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg      480

tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca      540

tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga      600

cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca      660

tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc      720

acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggccaac ttcaagatcc      780

gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca      840

tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga      900

gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg      960

ggatcactct cggcatggac gagctgtaca agcatcacca tcaccatcac tgagcggccg     1020

cactcgagag cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa     1080

atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt     1140

cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg     1200

gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga     1260

aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa     1320

atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac     1380

gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt     1440

ttgcgtttct acaaactctt tttgtttatt tttctaaata cattcaaata tgtatccgct     1500

catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat     1560

tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc     1620

tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg     1680

ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg     1740

ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga     1800

cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta     1860

ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc     1920

tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc     1980

gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg     2040

ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc     2100

aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca     2160

acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct     2220

tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat     2280

cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg     2340

gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat     2400

taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact     2460

tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat     2520

cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc     2580

ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct     2640

accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg     2700

cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca     2760

cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc     2820

tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga     2880

taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac     2940

gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga     3000

agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag     3060

ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg     3120

acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag     3180

caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc     3240

tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc     3300

tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct     3360

gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct     3420

cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt     3480

gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct     3540

tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt     3600

cagaggtttt caccgtcatc accgaaacgc gcgaggcagc agatcaattc gcgcgcgaag     3660

gcgaagcggc atgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta     3720

tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct     3780

acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat     3840

tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg     3900

gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg     3960

cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc     4020

gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg     4080

ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta     4140

atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa     4200

tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc     4260

tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc     4320

cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc     4380

tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt     4440

cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg     4500

agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc     4560

tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga     4620

tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt     4680

ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gct            4733


<210>  32
<211>  3005
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pUCDM

<400>  32
aattctgtca gccgttaagt gttcctgtgt cactgaaaat tgctttgaga ggctctaagg       60

gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt      120

aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt      180

tgctgattta tattaatttt atggtcaaac agagagctta gtacgtgaaa catgagagct      240

tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg      300

agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac      360

tatcaacagg ttgaactgct gatcaacaga tcctctacgc ggccgcggta ccataacttc      420

gtatagcata cattatacga agttatctgg tttaaacgta cccgtagtgg ctatggcagg      480

gcttgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg ggggttgggg      540

tggggaaaag gaagaaacgc gggcgtattg gtcccaatgg ggtctcggtg gggtatcgac      600

agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa cacccgtgcg      660

ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc      720

gtgtttcagt tagcctcccc catctcccgg taccgcatgc tatgcatcag ctgctagcac      780

catggctcga gatcccgggt gatcaagtct tcgtcgagtg attgtaaata aaatgtaatt      840

tacagtatag tattttaatt aatatacaaa tgatttgata ataattctta tttaactata      900

atatattgtg ttgggttgaa ttaaaggtcc gtatactagt atcgattcgc gacctactcc      960

ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta     1020

ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca     1080

taccgtccca ccatcgggcg cggatcccgg tccgaagcgc gcggaattca aaggcctacg     1140

tcgacgagct cactagtcgc ggccgctttc gaatctagag cctgcagtct cgacaagctt     1200

gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct     1260

ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt     1320

gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc     1380

acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta     1440

tcttatcatg tctggatctg atcactgctt gagcctagaa gatccggctg ctaacaaagc     1500

ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg     1560

ggcctctaaa cgggtcaacc cctaggggcc tctaaacggg tcttgagggg ttttttgctg     1620

aaaggaggaa ctatatccgg atctgaacag gagggacagc tgatagaaac agaagccact     1680

ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccgacgc     1740

actttgcgcc gaataaatac ctgtgacgga agatcacttc gcagaataaa taaatcctgg     1800

tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag acgttgatcg     1860

gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt     1920

tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata     1980

taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt     2040

tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt     2100

aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa     2160

tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt     2220

tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga     2280

ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg     2340

tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa     2400

tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc     2460

ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc     2520

gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt     2580

acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg     2640

cccttaaacg cctggtgcta cgcctgaata agtgataata agcggatgaa tggcagaaat     2700

tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg     2760

tctattgctg gtttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa     2820

atgcctgagg ccagtttgct caggctctcc ccgtggaggt aataattgac gatatgatca     2880

tttattctgc ctcccagagc ctgacattca tccggggtca gcaccgtttc tgcggactgg     2940

ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg     3000

aagct                                                                 3005


<210>  33
<211>  4717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pUCDM-PylRSWT-U6(Sf21)-2-tRNAPyl-3'term

<400>  33
aaccccgcgt ttatgaacaa acgacccaac acccgtgcgt tttattctgt ctttttattg       60

ccgtcatagc gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc      120

atctcccggt accgcatgct atgcatttac aggttcgtag agatcccgtt ataatacgat      180

tcagaacggg cggcacgttt gatgtttttg aagtcgtgtt tcactttcag caggcgttcc      240

agaccgaatc ctgctccgat ccacggtttg tcgatacccc actcacggtc cagcggaatt      300

ggtccaacaa cggcactaga cagttccagg tcgccgtgca tgacatccag ggtgtcgcca      360

tacaccatac agctgtcgcc cacaattttg aagtcaatgc ccaggtggtt cagaaaatcg      420

gtgatgatgc tttccaggtt ctcacgagta caacctgaac ccatttggca aaagttcagc      480

atggtaaact cctccagatg ttctttaccg tcggactctt tacgataaca cgggccgatc      540

tcgaagattt tgataggatc aggcagggca cggtccagtt tgcgcagata gttatacaga      600

tttggtgcca gcatagggcg cagacagaag tttttatcca cacggaaaat ttgtttgctc      660

agttcggtat cattgtcgat gcccatacgc tcgatatact ccagaggaat cagaatcggg      720

gatttgatct ccagaaagcc acgatccacg aaaaagcggg tgatttcacg ttccagtttc      780

cccagatagt tctcacgttc ttcggcatag atttgttgca ggtctttttt acgacgtgac      840

agcagttcgc tctccagttc acgaaacggt ttgccggaat tcaggctgat ttcgtctttc      900

ggattcagca gaacctccag acgatcggtt tgggattttg tcagtgctgg agctgatgct      960

tgaaccgggg cagacatgct tgtaatcgga ttggtattgc ctttaaccag ggcgctagcg     1020

gtggcaccgg tgctaatact gctaatgctg gtgctcacac ttgctggaac agaaacggac     1080

tcctgggtag aaacaggaat ggccggagag aatttgcttc cagacggctg tgcctgtgct     1140

gcttcagtgt tttccagtgg tttaggggca cgagcaacgg atttcggcat tgctttttta     1200

gtacgggtag gagcgctaac gactttcact ttcacgcttg tttggtcctc attggctttt     1260

gtcaggaatt tgttcagatc ctcatcggac acacggcaac gtttacaggt tttacgatat     1320

ttgtggtgac gcagtgcacg tgctgtacga gaagagcggc tattgttcac aaccagatga     1380

tcgccacacg ccatctcaat atagattttc gaacggctaa cctcgtggtg tttgatttta     1440

tgaatggttc cggtacgact catccacaga ccagtagcag agatcagggt attcagcggt     1500

tttttgtcca tggctcgaga tcccgggtga tcaagtcttc gtcgagtgat tgtaaataaa     1560

atgtaattta cagtatagta ttttaattaa tatacaaatg atttgataat aattcttatt     1620

taactataat atattgtgtt gggttgaatt aaaggtccgt atactagtat cgattcgcga     1680

cctactccgg aatattaata gacttaacta ctcaaaaagt gagggccagc agctcgacca     1740

atgtaaaacc ttgcgaggtg cgaggttacc ggggacccaa tcaaagagta taataactat     1800

agggaaaggc ccaacccccc ccccccccac tgtatgtaaa aatataagac ctatttctca     1860

acctataaac ctatgcaata aaacatccac tagattagtc tagtgactag actagaccat     1920

tgttagttaa cagtagttcg gctagatggc gccaaattgg ttcttttagt gaacggtaga     1980

tggcgctgta ctcaatcttc atacaaatca tgttaaatgt atgggattct acatcgcgct     2040

atcaaagttt tcattgtgtt tgtgaagggt acaataattt tgccttggca agtggaaacc     2100

tgatcatgta gatcgaatgg actctaaatc cgttcagccg ggttagattc ccggggtttc     2160

cgttttttga agagtttcag tttggtatgg tttttctatt ttcaaattgg tatgagggag     2220

taagcataat caaatttaat ttcttttgtt aaactttagc tttctagagc ctgcagtctc     2280

gacaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt     2340

ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca     2400

attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc     2460

acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc     2520

atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctagaag atccggctgc     2580

taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata     2640

accccttggg gcctctaaac gggtcaaccc ctaggggcct ctaaacgggt cttgaggggt     2700

tttttgctga aaggaggaac tatatccgga tctgaacagg agggacagct gatagaaaca     2760

gaagccactg gagcacctca aaaacaccat catacactaa atcagtaagt tggcagcatc     2820

acccgacgca ctttgcgccg aataaatacc tgtgacggaa gatcacttcg cagaataaat     2880

aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga     2940

cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg     3000

cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat     3060

cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt     3120

tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt     3180

aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg     3240

cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg     3300

ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct     3360

ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc     3420

gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt     3480

ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa     3540

cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat     3600

gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct     3660

taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag     3720

ttattggtgc ccttaaacgc ctggtgctac gcctgaataa gtgataataa gcggatgaat     3780

ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt cgttaaatag     3840

ccgcttatgt ctattgctgg tttaccggtt tattgactac cggaagcagt gtgaccgtgt     3900

gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta ataattgacg     3960

atatgatcat ttattctgcc tcccagagcc tgacattcat ccggggtcag caccgtttct     4020

gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc     4080

ggcagcgtga agctaattct gtcagccgtt aagtgttcct gtgtcactca aaattgcttt     4140

gagaggctct aagggcttct cagtgcgtta catccctggc ttgttgtcca caaccgttaa     4200

accttaaaag ctttaaaagc cttatatatt cttttttttc ttataaaact taaaacctta     4260

gaggctattt aagttgctga tttatattaa ttttatttgt caaacatgag agcttagtac     4320

gtgaaacatg agagcttagt acgttagcca tgagagctta gtacgttagc catgagggtt     4380

tagttcgtta aacatgagag cttagtacgt taaacatgag agcttagtac gtgaaacatg     4440

agagcttagt acgtactatc aacaggttga actgctgatc aacagatcct ctacgcggcc     4500

gcggtaccat aacttcgtat agcatacatt atacgaagtt atctggttta aacgtacccg     4560

tagtggctat ggcagggctt gccgccccga cgttggctgc gagccctggg ccttcacccg     4620

aacttggggg ttggggtggg gaaaaggaag aaacgcgggc gtattggtcc caatggggtc     4680

tcggtggggt atcgacagag tgccagccct gggaccg                              4717


<210>  34
<211>  4713
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pUCDM-PylRSAF-U6(Sf21)-2-tRNAPyl-3'term

<400>  34
tcgagatgga caaaaaaccg ctgaataccc tgatctctgc tactggtctg tggatgagtc       60

gtaccggaac cattcataaa atcaaacacc acgaggttag ccgttcgaaa atctatattg      120

agatggcgtg tggcgatcat ctggttgtga acaatagccg ctcttctcgt acagcacgtg      180

cactgcgtca ccacaaatat cgtaaaacct gtaaacgttg ccgtgtgtcc gatgaggatc      240

tgaacaaatt cctgacaaaa gccaatgagg accaaacaag cgtgaaagtg aaagtcgtta      300

gcgctcctac ccgtactaaa aaagcaatgc cgaaatccgt tgctcgtgcc cctaaaccac      360

tggaaaacac tgaagcagca caggcacagc cgtctggaag caaattctct ccggccattc      420

ctgtttctac ccaggagtcc gtttctgttc cagcaagtgt gagcaccagc attagcagta      480

ttagcaccgg tgccaccgct agcgccctgg ttaaaggcaa taccaatccg attacaagca      540

tgtctgcccc ggttcaagca tcagctccag cactgacaaa atcccaaacc gatcgtctgg      600

aggttctgct gaatccgaaa gacgaaatca gcctgaattc cggcaaaccg tttcgtgaac      660

tggagagcga actgctgtca cgtcgtaaaa aagacctgca acaaatctat gccgaagaac      720

gtgagaacta tctggggaaa ctggaacgtg aaatcacccg ctttttcgtg gatcgtggct      780

ttctggagat caaatccccg attctgattc ctctggagta tatcgagcgt atgggcatcg      840

acaatgatac cgaactgagc aaacaaattt tccgtgtgga taaaaacttc tgtctgcgcc      900

ctatgcttgc accaaatctg gctaactatc tgcgcaaact ggaccgtgcc ctgcctgatc      960

ctatcaaaat cttcgagatc ggcccgtgtt atcgtaaaga gtccgacggt aaagaacatc     1020

tggaggagtt taccatgctg aacttttgcc aaatgggttc aggttgtact cgtgagaacc     1080

tggaaagcat catcaccgat tttctgaacc acctgggcat tgacttcaaa attgtgggcg     1140

acagctgtat ggtgtttggc gacaccctgg atgtcatgca cggcgacctg gaactgtcta     1200

gtgccgttgt tggaccaatt ccgctggacc gtgagtgggg tatcgacaaa ccgtggatcg     1260

gagcaggatt cggtctggaa cgcctgctga aagtgaaaca cgacttcaaa aacatcaaac     1320

gtgccgcccg ttctgaatcg tattataacg ggatctctac gaacctgtaa atgcatagca     1380

tgcggtaccg ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga     1440

acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt     1500

tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca     1560

ttgggaccaa tacgcccgcg tttcttcctt ttccccaccc caacccccaa gttcgggtga     1620

aggcccaggg ctcgcagcca acgtcggggc ggcaagccct gccatagcca ctacgggtac     1680

gtttaaacca gataacttcg tataatgtat gctatacgaa gttatggtac cgcggccgcg     1740

tagaggatct gttgatcagc agttcaacct gttgatagta cgtactaagc tctcatgttt     1800

cacgtactaa gctctcatgt ttaacgtact aagctctcat gtttaacgaa ctaaaccctc     1860

atggctaacg tactaagctc tcatggctaa cgtactaagc tctcatgttt cacgtactaa     1920

gctctctgtt tgaccataaa attaatataa atcagcaact taaatagcct ctaaggtttt     1980

aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg tttaacggtt     2040

gtggacaaca agccagggat gtaacgcact gagaagccct tagagcctct caaagcaatt     2100

ttcagtgaca caggaacact taacggctga cagaattagc ttcacgctgc cgcaagcact     2160

cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt     2220

gctgaccccg gatgaatgtc aggctctggg aggcagaata aatgatcata tcgtcaatta     2280

ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca     2340

cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac     2400

gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg     2460

cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa     2520

aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg     2580

ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc     2640

ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata     2700

ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac     2760

atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct     2820

tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa     2880

aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc     2940

agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga     3000

atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc     3060

gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca     3120

aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc     3180

tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt     3240

gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt     3300

cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct     3360

gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc     3420

aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat     3480

cagctgtccc tcctgttcag atccggatat agttcctcct ttcagcaaaa aacccctcaa     3540

gacccgttta gaggccccta ggggttgacc cgtttagagg ccccaagggg ttatgctagt     3600

tattgctcag cggtggcagc agccaactca gcttcctttc gggctttgtt agcagccgga     3660

tcttctaggc tcaagcagtg atcagatcca gacatgataa gatacattga tgagtttgga     3720

caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt     3780

gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat     3840

tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac     3900

aaatgtggta tggctgatta tgatcctcta gtacttctcg acaagcttgt cgagactgca     3960

ggctctagaa agctaaagtt taacaaaaga aattaaattt gattatgctt actccctcat     4020

accaatttga aaatagaaaa accataccaa actgaaactc ttcaaaaaac ggaaaccccg     4080

ggaatctaac ccggctgaac ggatttagag tccattcgat ctacatgatc aggtttccac     4140

ttgccaaggc aaaattattg tacccttcac aaacacaatg aaaactttga tagcgcgatg     4200

tagaatccca tacatttaac atgatttgta tgaagattga gtacagcgcc atctaccgtt     4260

cactaaaaga accaatttgg cgccatctag ccgaactact gttaactaac aatggtctag     4320

tctagtcact agactaatct agtggatgtt ttattgcata ggtttatagg ttgagaaata     4380

ggtcttatat ttttacatac agtggggggg gggggggttg ggcctttccc tatagttatt     4440

atactctttg attgggtccc cggtaacctc gcacctcgca aggttttaca ttggtcgagc     4500

tgctggccct cactttttga gtagttaagt ctattaatat tccggagtag gtcgcgaatc     4560

gatactagta tacggacctt taattcaacc caacacaata tattatagtt aaataagaat     4620

tattatcaaa tcatttgtat attaattaaa atactatact gtaaattaca ttttatttac     4680

aatcactcga cgaagacttg atcacccggg atc                                  4713


<210>  35
<211>  4412
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pACEBac-DUAL

<400>  35
ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga       60

ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc      120

attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct      180

agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg caaactatta      240

actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat      300

aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa      360

tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag      420

ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat      480

agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt      540

tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg      600

aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga      660

gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta      720

atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa      780

gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact      840

gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca      900

tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt      960

accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg     1020

ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag     1080

cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta     1140

agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat     1200

ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg     1260

tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc     1320

ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac     1380

cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc     1440

gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg     1500

tgcggtattt cacaccgcat agaccagccg cgtaacctgg caaaatcggt tacggttgag     1560

taataaatgg atgccctgcg taagcgggtg tgggcggaca ataaagtctt aaactgaaca     1620

aaatagatct aaactatgac aataaagtct taaactagac agaatagttg taaactgaaa     1680

tcagtccagt tatgctgtga aaaagcatac tggacttttg ttatggctaa agcaaactct     1740

tcattttctg aagtgcaaat tgcccgtcgt attaaagagg ggcgtggcca agggcatggt     1800

aaagactata ttcgcggcgt tgtgacaatt taccgaacaa ctccgcggcc gggaagccga     1860

tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt     1920

cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt     1980

gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg     2040

cggtggcaat gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct     2100

cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt     2160

cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat     2220

cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat     2280

caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc     2340

ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc     2400

tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct     2460

tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa     2520

accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag     2580

cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt     2640

gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag     2700

gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca     2760

ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc cttgcttcag     2820

gagatcggta gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg     2880

gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga ctctagctat     2940

agttctagtg gttggctaca gctttgtttg tactatcaac aggttgaact gctgatcaac     3000

agatcctcta cgcggccgcg gtaccataac ttcgtatagc atacattata cgaagttatc     3060

tggtttaaac gtacccgtag tggctatggc agggcttgcc gccccgacgt tggctgcgag     3120

ccctgggcct tcacccgaac ttgggggttg gggtggggaa aaggaagaaa cgcgggcgta     3180

ttggtcccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc     3240

gcgtttatga acaaacgacc caacacccgt gcgttttatt ctgtcttttt attgccgtca     3300

tagcgcgggt tccttccggt attgtctcct tccgtgtttc agttagcctc ccccatctcc     3360

cggtaccgca tgctatgcat cagctgctag caccatggct cgagatcccg ggtgatcaag     3420

tcttcgtcga gtgattgtaa ataaaatgta atttacagta tagtatttta attaatatac     3480

aaatgatttg ataataattc ttatttaact ataatatatt gtgttgggtt gaattaaagg     3540

tccgtacgac ctactccgga atattaatag atcatggaga taattaaaat gataaccatc     3600

tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa     3660

atattccgga ttattcatac cgtcccacca tcgggcgcgg atcccggtcc gaagcgcgcg     3720

gaattcaaag gcctacgtcg acgagctcac tagtcgcggc cgctttcgaa tctagagcct     3780

gcagtctcga caagcttgtc gagaagtact agaggatcat aatcagccat accacatttg     3840

tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa     3900

tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca     3960

atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt     4020

ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag cctagaagat     4080

ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa     4140

ctatcataac ccctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg     4200

tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac     4260

tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg     4320

tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact     4380

ccatgtgaca aaccgtcatc ttcggctact tt                                   4412


<210>  36
<211>  5100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pACEBac-DUAL-GFP(Y39TAG)-6His

<400>  36
ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga       60

ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc      120

attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct      180

agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg caaactatta      240

actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat      300

aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa      360

tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag      420

ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat      480

agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt      540

tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg      600

aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga      660

gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta      720

atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa      780

gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact      840

gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca      900

tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt      960

accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg     1020

ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag     1080

cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta     1140

agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat     1200

ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg     1260

tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc     1320

ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac     1380

cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc     1440

gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg     1500

tgcggtattt cacaccgcat agaccagccg cgtaacctgg caaaatcggt tacggttgag     1560

taataaatgg atgccctgcg taagcgggtg tgggcggaca ataaagtctt aaactgaaca     1620

aaatagatct aaactatgac aataaagtct taaactagac agaatagttg taaactgaaa     1680

tcagtccagt tatgctgtga aaaagcatac tggacttttg ttatggctaa agcaaactct     1740

tcattttctg aagtgcaaat tgcccgtcgt attaaagagg ggcgtggcca agggcatggt     1800

aaagactata ttcgcggcgt tgtgacaatt taccgaacaa ctccgcggcc gggaagccga     1860

tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt     1920

cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt     1980

gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg     2040

cggtggcaat gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct     2100

cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt     2160

cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat     2220

cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat     2280

caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc     2340

ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc     2400

tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct     2460

tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa     2520

accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag     2580

cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt     2640

gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag     2700

gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca     2760

ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc cttgcttcag     2820

gagatcggta gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg     2880

gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga ctctagctat     2940

agttctagtg gttggctaca gctttgtttg tactatcaac aggttgaact gctgatcaac     3000

agatcctcta cgcggccgcg gtaccataac ttcgtatagc atacattata cgaagttatc     3060

tggtttaaac gtacccgtag tggctatggc agggcttgcc gccccgacgt tggctgcgag     3120

ccctgggcct tcacccgaac ttgggggttg gggtggggaa aaggaagaaa cgcgggcgta     3180

ttggtcccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc     3240

gcgtttatga acaaacgacc caacacccgt gcgttttatt ctgtcttttt attgccgtca     3300

tagcgcgggt tccttccggt attgtctcct tccgtgtttc agttagcctc ccccatctcc     3360

cggtaccgca tgctatgcat cagctgctag caccatggct cgagatcccg ggtgatcaag     3420

tcttcgtcga gtgattgtaa ataaaatgta atttacagta tagtatttta attaatatac     3480

aaatgatttg ataataattc ttatttaact ataatatatt gtgttgggtt gaattaaagg     3540

tccgtacgac ctactccgga atattaatag atcatggaga taattaaaat gataaccatc     3600

tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa     3660

atattccgga ttattcatac cgtcccacca tcgggcgcgg atccatggat tacaaagatg     3720

atgatgataa agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg     3780

agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg     3840

ccacctaggg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct     3900

ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc     3960

acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca     4020

ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg     4080

acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc     4140

tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc     4200

agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc     4260

agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg     4320

acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc     4380

acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt     4440

acaagcatca ccatcaccat cactgactgc agtctcgaca agcttgtcga gaagtactag     4500

aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca     4560

cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt     4620

gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt     4680

ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg     4740

atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa aggaagctga     4800

gttggctgct gccaccgctg agcaataact atcataaccc ctaggagatc cgaaccagat     4860

aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc     4920

tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt     4980

ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt     5040

atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt     5100


<210>  37
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDS of Herceptin heavy chain- 6His


<220>
<221>  CDS
<222>  (1)..(717)

<400>  37
atg gaa gtg cag ctg gtc gag tcc ggt ggt ggc ctg gtt cag cct ggt         48
Met Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly           
1               5                   10                  15                

ggt tcc ctg cgt ctg tcc tgc gct gct tcc ggt ttc aac atc aag gac         96
Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp           
            20                  25                  30                    

acc tac atc cac tgg gtc cgc cag gct ccc ggc aag gga ttg gaa tgg        144
Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp           
        35                  40                  45                        

gtg gcc cgt atc tac ccc acc aac ggt tac acc cgt tac gct gac tcc        192
Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser           
    50                  55                  60                            

gtg aag ggc cgt ttc acc atc tcc gct gac acc tcc aag aac acc gct        240
Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala           
65                  70                  75                  80            

tac ctg cag atg aac tcc ctg cgt gct gag gac acc gct gtg tac tac        288
Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr           
                85                  90                  95                

tgc tcc cgt tgg ggt ggc gac ggt ttc tac gct atg gac tac tgg ggc        336
Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly           
            100                 105                 110                   

cag ggc acc ctg gtc acc gtg tcc tct gct tcc acc aag ggc ccc tcc        384
Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser           
        115                 120                 125                       

gtg ttc cct ctg gct cca tcc tcc aag tcc acc tcc ggt gga acc gct        432
Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala           
    130                 135                 140                           

gct ctg ggt tgc ctg gtc aag gac tac ttc ccc gag ccc gtg acc gtg        480
Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val           
145                 150                 155                 160           

tct tgg aac tcc ggt gct ctg acc tcc ggc gtg cac acc ttc cct gct        528
Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala           
                165                 170                 175               

gtc ctg cag tcc tcc ggc ctg tac tcc ctg tcc tcc gtc gtg act gtg        576
Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val           
            180                 185                 190                   

ccc tca tcc tcc ctg ggc acc cag acc tac atc tgc aac gtg aac cac        624
Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His           
        195                 200                 205                       

aag ccc tcc aac acc aag gtg gac aag aag gtc gag ccc ccc aag tcc        672
Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Pro Lys Ser           
    210                 215                 220                           

tgc gac aag acc cac act tgc ccc cct cat cac cat cac cac cac            717
Cys Asp Lys Thr His Thr Cys Pro Pro His His His His His His               
225                 230                 235                               


<210>  38
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  38

Met Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15      


Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp 
            20                  25                  30          


Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
        115                 120                 125             


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
    130                 135                 140                 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
145                 150                 155                 160 


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
                165                 170                 175     


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
            180                 185                 190         


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
        195                 200                 205             


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Pro Lys Ser 
    210                 215                 220                 


Cys Asp Lys Thr His Thr Cys Pro Pro His His His His His His 
225                 230                 235                 


<210>  39
<211>  648
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDS Herceptin light chain


<220>
<221>  CDS
<222>  (1)..(648)

<400>  39
atg gac atc cag atg acc cag tcc ccc tcc tcc ctg tcc gct tcc gtg         48
Met Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val           
1               5                   10                  15                

gga gat cgt gtg acc atc act tgc cgt gct tcc cag gac gtg aac acc         96
Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr           
            20                  25                  30                    

gct gtg gct tgg tac cag cag aag ccc ggc aag gct ccc aag ctg ctg        144
Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu           
        35                  40                  45                        

atc tac tcc gct agc ttc ctg tac tcc ggt gtc ccc tcc cgt ttc tcc        192
Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser           
    50                  55                  60                            

ggt tcc cgt tcc ggt act gac ttc acc ctg acc atc tcc agc ctg cag        240
Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln           
65                  70                  75                  80            

ccc gag gac ttc gct acc tac tac tgc cag cag cac tac acc acc ccc        288
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro           
                85                  90                  95                

ccc acc ttc ggc cag ggt act aag gtc gag atc aag cgt acc gtg gct        336
Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala           
            100                 105                 110                   

gct ccc tcc gtg ttc atc ttc cca ccc tcc gac gag cag ctg aag tcc        384
Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser           
        115                 120                 125                       

ggc act gct tcc gtc gtg tgc ctg ctg aac aac ttc tac ccc cgc gag        432
Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu           
    130                 135                 140                           

gct aag gtg cag tgg aag gtg gac aac gct ctg cag tcc ggc aac tcc        480
Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser           
145                 150                 155                 160           

caa gag tcc gtg acc gag cag gac tcc aag gac tct acc tac tct ctg        528
Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu           
                165                 170                 175               

tcc tct acc ctg acc ctg tcc aag gct gac tac gag aag cac aag gtg        576
Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val           
            180                 185                 190                   

tac gct tgc gaa gtg acc cac cag ggc ctg tcc tcc cca gtg acc aag        624
Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys           
        195                 200                 205                       

tcc ttc aac cgt ggc gag tgc taa                                        648
Ser Phe Asn Arg Gly Glu Cys                                               
    210                 215                                               


<210>  40
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  40

Met Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
1               5                   10                  15      


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr 
            20                  25                  30          


Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser 
    50                  55                  60                  


Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro 
                85                  90                  95      


Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala 
            100                 105                 110         


Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser 
        115                 120                 125             


Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu 
    130                 135                 140                 


Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser 
145                 150                 155                 160 


Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu 
                165                 170                 175     


Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val 
            180                 185                 190         


Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys 
        195                 200                 205             


Ser Phe Asn Arg Gly Glu Cys 
    210                 215 


<210>  41
<211>  5747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pAceBac-DUAL-Herceptin-6His

<400>  41
gatccatgga catccagatg acccagtccc cctcctccct gtccgcttcc gtgggagatc       60

gtgtgaccat cacttgccgt gcttcccagg acgtgaacac cgctgtggct tggtaccagc      120

agaagcccgg caaggctccc aagctgctga tctactccgc tagcttcctg tactccggtg      180

tcccctcccg tttctccggt tcccgttccg gtactgactt caccctgacc atctccagcc      240

tgcagcccga ggacttcgct acctactact gccagcagca ctacaccacc ccccccacct      300

tcggccaggg tactaaggtc gagatcaagc gtaccgtggc tgctccctcc gtgttcatct      360

tcccaccctc cgacgagcag ctgaagtccg gcactgcttc cgtcgtgtgc ctgctgaaca      420

acttctaccc ccgcgaggct aaggtgcagt ggaaggtgga caacgctctg cagtccggca      480

actcccaaga gtccgtgacc gagcaggact ccaaggactc tacctactct ctgtcctcta      540

ccctgaccct gtccaaggct gactacgaga agcacaaggt gtacgcttgc gaagtgaccc      600

accagggcct gtcctcccca gtgaccaagt ccttcaaccg tggcgagtgc taagaattca      660

aaggcctacg tcgacgagct cactagtcgc ggccgctttc gaatctagag cctgcagtct      720

cgacaagctt gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt      780

tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc      840

aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat      900

cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact      960

catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagaa gatccggctg     1020

ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactatcat     1080

aacccctagg agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt     1140

taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc     1200

taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc     1260

acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg     1320

acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct     1380

cttcgttatt aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg     1440

aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg     1500

tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc     1560

tcgccacgtt cgccgcaaac tattaactgg cgaactactt actctagctt cccggcaaca     1620

attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc     1680

ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat     1740

tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag     1800

tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa     1860

gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca     1920

tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc     1980

ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc     2040

ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc     2100

agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt     2160

cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt     2220

caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc     2280

tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa     2340

ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac     2400

ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg     2460

gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga     2520

gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact     2580

tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa     2640

cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc     2700

gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg     2760

ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat     2820

gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagacc agccgcgtaa     2880

cctggcaaaa tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc     2940

ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac     3000

tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac     3060

ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa     3120

agaggggcgt ggccaagggc atggtaaaga ctatattcgc ggcgttgtga caatttaccg     3180

aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt     3240

gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact     3300

gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc     3360

tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag     3420

actgcgagat catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa     3480

gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta     3540

ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg     3600

tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg     3660

ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga     3720

ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa     3780

acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa     3840

aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt     3900

caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga     3960

acaggcttat gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc     4020

aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt     4080

ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct     4140

gtgcacggat ctgcccttgc ttcaggagat cggtagacct cggccgtcgc ggcgcttgcc     4200

ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg     4260

tttgttcgcc caggactcta gctatagttc tagtggttgg ctacagcttt gtttgtacta     4320

tcaacaggtt gaactgctga tcaacagatc ctctacgcgg ccgcggtacc ataacttcgt     4380

atagcataca ttatacgaag ttatctggtt taaacgtacc cgtagtggct atggcagggc     4440

ttgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg ggttggggtg     4500

gggaaaagga agaaacgcgg gcgtattggt cccaatgggg tctcggtggg gtatcgacag     4560

agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt     4620

ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt     4680

gtttcagtta gcctccccca tctcccggta ccgcatgcta tgcatcagct gctagcttag     4740

tggtggtgat ggtgatgagg ggggcaagtg tgggtcttgt cgcaggactt ggggggctcg     4800

accttcttgt ccaccttggt gttggagggc ttgtggttca cgttgcagat gtaggtctgg     4860

gtgcccaggg aggatgaggg cacagtcacg acggaggaca gggagtacag gccggaggac     4920

tgcaggacag cagggaaggt gtgcacgccg gaggtcagag caccggagtt ccaagacacg     4980

gtcacgggct cggggaagta gtccttgacc aggcaaccca gagcagcggt tccaccggag     5040

gtggacttgg aggatggagc cagagggaac acggaggggc ccttggtgga agcagaggac     5100

acggtgacca gggtgccctg gccccagtag tccatagcgt agaaaccgtc gccaccccaa     5160

cgggagcagt agtacacagc ggtgtcctca gcacgcaggg agttcatctg caggtaagcg     5220

gtgttcttgg aggtgtcagc ggagatggtg aaacggccct tcacggagtc agcgtaacgg     5280

gtgtaaccgt tggtggggta gatacgggcc acccattcca atcccttgcc gggagcctgg     5340

cggacccagt ggatgtaggt gtccttgatg ttgaaaccgg aagcagcgca ggacagacgc     5400

agggaaccac caggctgaac caggccacca ccggactcga ccagctgcac ttccatcccg     5460

ggtgatcaag tcttcgtcga gtgattgtaa ataaaatgta atttacagta tagtatttta     5520

attaatatac aaatgatttg ataataattc ttatttaact ataatatatt gtgttgggtt     5580

gaattaaagg tccgcgacct actccggaat attaatagat catggagata attaaaatga     5640

taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa     5700

acctataaat attccggatt attcataccg tcccaccatc gggcgcg                   5747


<210>  42
<211>  6349
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pFastBac-Dual-6HisTAF11/TAF13

<400>  42
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcct acgtacccgt agtggctatg     3900

gcagggcttg ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggt     3960

tggggtgggg aaaaggaaga aacgcgggcg tattggtccc aatggggtct cggtggggta     4020

tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc     4080

gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc     4140

cttccgtgtt tcagttagcc tcccccatct cccggtaccg catgctatgc atcagaacaa     4200

ttgcggccgc ggatcctcaa gatccataat ttgcttcatc aaatgctttt ctagctcgtt     4260

tcaattcttc attcatagta agcaagtctt taaccctggc aaacttcctt gggtcctttc     4320

gaatcaagaa gacgatatct tcaacttgta ctcgaccttg tcttccaatt gacattgcct     4380

tgtgagtcat ttcagtgata aactctatga caagatcttc aagaatatcc actgactcag     4440

tataaggatt ctggtcatcc ccaaagccat acatcataca tcgcaattct ttagaaaaaa     4500

gtctctttct tttaccctgt ccaccttctg cacctcctcc aatttcttca ttttcttcct     4560

caaacgtggg gtcttcttcc tcatctgcca tatgttctcg agatcccggg tgatcaagtc     4620

ttcgtcgagt gattgtaaat aaaatgtaat ttacagtata gtattttaat taatatacaa     4680

atgatttgat aataattctt atttaactat aatatattgt gttgggttga attaaaggtc     4740

cgtactccgg aatattaata gatcatggag ataattaaaa tgataaccat ctcgcaaata     4800

aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatattccgg     4860

attattcata ccgtcccacc atcgggcgcg gatctcggtc cgaaaccatg tcgtactacc     4920

atcaccatca ccatcacgat tacgatatcc caacgaccga aaacctgtat tttcagggcg     4980

ccatggacga tgcccacgag tcgccctccg acaaaggtgg agagacaggg gagtcggatg     5040

agacggccgc tgtgcccggg gacccggggg ctaccgacac cgatggaatc ccagaggaaa     5100

ctgacggaga cgcagatgtg gacttgaaag aagctgcagc ggaggaaggc gagctcgaga     5160

gtcaggatgt ctcagattta acaacagttg aaagggaaga ctcatcatta cttaatcctg     5220

cagccaaaaa actgaaaata gataccaaag aaaagaaaga gaaaaagcag aaagtagatg     5280

aagatgagat tcagaagatg caaatcctgg tttcttcttt ttctgaggag cagctgaacc     5340

gttatgaaat gtatcgccgc tcagctttcc ctaaggcagc catcaaaagg ctgatccagt     5400

ccatcactgg cacctctgtg tctcagaatg ttgttattgc tatgtctggt atttccaagg     5460

ttttcgtcgg ggaggtggta gaagaagcac tggatgtgtg tgagaagtgg ggagaaatgc     5520

caccactaca acccaaacat atgagggaag ccgttagaag gttaaagtca aaaggacaga     5580

tccctaactc gaagcacaaa aaaatcatct tcttctaagg atccggaatt caaaggccta     5640

cgtcgacgag ctcaactagt gcggccgctt tcgaatctag agcctgcagt ctcgaggcat     5700

gcggtaccaa gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag     5760

aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga     5820

atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata     5880

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca     5940

aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct aggagatccg     6000

aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct     6060

tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa     6120

ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg ggcatttttc     6180

ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc gtcatcttcg     6240

gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt attaatgttt     6300

gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatggg                 6349


<210>  43
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDS 6His-TAF11


<220>
<221>  CDS
<222>  (1)..(711)

<400>  43
atg tcg tac tac cat cac cat cac cat cac gat tac gat atc cca acg         48
Met Ser Tyr Tyr His His His His His His Asp Tyr Asp Ile Pro Thr           
1               5                   10                  15                

acc gaa aac ctg tat ttt cag ggc gcc atg gac gat gcc cac gag tcg         96
Thr Glu Asn Leu Tyr Phe Gln Gly Ala Met Asp Asp Ala His Glu Ser           
            20                  25                  30                    

ccc tcc gac aaa ggt gga gag aca ggg gag tcg gat gag acg gcc gct        144
Pro Ser Asp Lys Gly Gly Glu Thr Gly Glu Ser Asp Glu Thr Ala Ala           
        35                  40                  45                        

gtg ccc ggg gac ccg ggg gct acc gac acc gat gga atc cca gag gaa        192
Val Pro Gly Asp Pro Gly Ala Thr Asp Thr Asp Gly Ile Pro Glu Glu           
    50                  55                  60                            

act gac gga gac gca gat gtg gac ttg aaa gaa gct gca gcg gag gaa        240
Thr Asp Gly Asp Ala Asp Val Asp Leu Lys Glu Ala Ala Ala Glu Glu           
65                  70                  75                  80            

ggc gag ctc gag agt cag gat gtc tca gat tta aca aca gtt gaa agg        288
Gly Glu Leu Glu Ser Gln Asp Val Ser Asp Leu Thr Thr Val Glu Arg           
                85                  90                  95                

gaa gac tca tca tta ctt aat cct gca gcc aaa aaa ctg aaa ata gat        336
Glu Asp Ser Ser Leu Leu Asn Pro Ala Ala Lys Lys Leu Lys Ile Asp           
            100                 105                 110                   

acc aaa gaa aag aaa gag aaa aag cag aaa gta gat gaa gat gag att        384
Thr Lys Glu Lys Lys Glu Lys Lys Gln Lys Val Asp Glu Asp Glu Ile           
        115                 120                 125                       

cag aag atg caa atc ctg gtt tct tct ttt tct gag gag cag ctg aac        432
Gln Lys Met Gln Ile Leu Val Ser Ser Phe Ser Glu Glu Gln Leu Asn           
    130                 135                 140                           

cgt tat gaa atg tat cgc cgc tca gct ttc cct aag gca gcc atc aaa        480
Arg Tyr Glu Met Tyr Arg Arg Ser Ala Phe Pro Lys Ala Ala Ile Lys           
145                 150                 155                 160           

agg ctg atc cag tcc atc act ggc acc tct gtg tct cag aat gtt gtt        528
Arg Leu Ile Gln Ser Ile Thr Gly Thr Ser Val Ser Gln Asn Val Val           
                165                 170                 175               

att gct atg tct ggt att tcc aag gtt ttc gtc ggg gag gtg gta gaa        576
Ile Ala Met Ser Gly Ile Ser Lys Val Phe Val Gly Glu Val Val Glu           
            180                 185                 190                   

gaa gca ctg gat gtg tgt gag aag tgg gga gaa atg cca cca cta caa        624
Glu Ala Leu Asp Val Cys Glu Lys Trp Gly Glu Met Pro Pro Leu Gln           
        195                 200                 205                       

ccc aaa cat atg agg gaa gcc gtt aga agg tta aag tca aaa gga cag        672
Pro Lys His Met Arg Glu Ala Val Arg Arg Leu Lys Ser Lys Gly Gln           
    210                 215                 220                           

atc cct aac tcg aag cac aaa aaa atc atc ttc ttc taa                    711
Ile Pro Asn Ser Lys His Lys Lys Ile Ile Phe Phe                           
225                 230                 235                               


<210>  44
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  44

Met Ser Tyr Tyr His His His His His His Asp Tyr Asp Ile Pro Thr 
1               5                   10                  15      


Thr Glu Asn Leu Tyr Phe Gln Gly Ala Met Asp Asp Ala His Glu Ser 
            20                  25                  30          


Pro Ser Asp Lys Gly Gly Glu Thr Gly Glu Ser Asp Glu Thr Ala Ala 
        35                  40                  45              


Val Pro Gly Asp Pro Gly Ala Thr Asp Thr Asp Gly Ile Pro Glu Glu 
    50                  55                  60                  


Thr Asp Gly Asp Ala Asp Val Asp Leu Lys Glu Ala Ala Ala Glu Glu 
65                  70                  75                  80  


Gly Glu Leu Glu Ser Gln Asp Val Ser Asp Leu Thr Thr Val Glu Arg 
                85                  90                  95      


Glu Asp Ser Ser Leu Leu Asn Pro Ala Ala Lys Lys Leu Lys Ile Asp 
            100                 105                 110         


Thr Lys Glu Lys Lys Glu Lys Lys Gln Lys Val Asp Glu Asp Glu Ile 
        115                 120                 125             


Gln Lys Met Gln Ile Leu Val Ser Ser Phe Ser Glu Glu Gln Leu Asn 
    130                 135                 140                 


Arg Tyr Glu Met Tyr Arg Arg Ser Ala Phe Pro Lys Ala Ala Ile Lys 
145                 150                 155                 160 


Arg Leu Ile Gln Ser Ile Thr Gly Thr Ser Val Ser Gln Asn Val Val 
                165                 170                 175     


Ile Ala Met Ser Gly Ile Ser Lys Val Phe Val Gly Glu Val Val Glu 
            180                 185                 190         


Glu Ala Leu Asp Val Cys Glu Lys Trp Gly Glu Met Pro Pro Leu Gln 
        195                 200                 205             


Pro Lys His Met Arg Glu Ala Val Arg Arg Leu Lys Ser Lys Gly Gln 
    210                 215                 220                 


Ile Pro Asn Ser Lys His Lys Lys Ile Ile Phe Phe 
225                 230                 235     


<210>  45
<211>  375
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDS TAF13


<220>
<221>  CDS
<222>  (1)..(375)

<400>  45
atg gca gat gag gaa gaa gac ccc acg ttt gag gaa gaa aat gaa gaa         48
Met Ala Asp Glu Glu Glu Asp Pro Thr Phe Glu Glu Glu Asn Glu Glu           
1               5                   10                  15                

att gga gga ggt gca gaa ggt gga cag ggt aaa aga aag aga ctt ttt         96
Ile Gly Gly Gly Ala Glu Gly Gly Gln Gly Lys Arg Lys Arg Leu Phe           
            20                  25                  30                    

tct aaa gaa ttg cga tgt atg atg tat ggc ttt ggg gat gac cag aat        144
Ser Lys Glu Leu Arg Cys Met Met Tyr Gly Phe Gly Asp Asp Gln Asn           
        35                  40                  45                        

cct tat act gag tca gtg gat att ctt gaa gat ctt gtc ata gag ttt        192
Pro Tyr Thr Glu Ser Val Asp Ile Leu Glu Asp Leu Val Ile Glu Phe           
    50                  55                  60                            

atc act gaa atg act cac aag gca atg tca att gga aga caa ggt cga        240
Ile Thr Glu Met Thr His Lys Ala Met Ser Ile Gly Arg Gln Gly Arg           
65                  70                  75                  80            

gta caa gtt gaa gat atc gtc ttc ttg att cga aag gac cca agg aag        288
Val Gln Val Glu Asp Ile Val Phe Leu Ile Arg Lys Asp Pro Arg Lys           
                85                  90                  95                

ttt gcc agg gtt aaa gac ttg ctt act atg aat gaa gaa ttg aaa cga        336
Phe Ala Arg Val Lys Asp Leu Leu Thr Met Asn Glu Glu Leu Lys Arg           
            100                 105                 110                   

gct aga aaa gca ttt gat gaa gca aat tat gga tct tga                    375
Ala Arg Lys Ala Phe Asp Glu Ala Asn Tyr Gly Ser                           
        115                 120                                           


<210>  46
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  46

Met Ala Asp Glu Glu Glu Asp Pro Thr Phe Glu Glu Glu Asn Glu Glu 
1               5                   10                  15      


Ile Gly Gly Gly Ala Glu Gly Gly Gln Gly Lys Arg Lys Arg Leu Phe 
            20                  25                  30          


Ser Lys Glu Leu Arg Cys Met Met Tyr Gly Phe Gly Asp Asp Gln Asn 
        35                  40                  45              


Pro Tyr Thr Glu Ser Val Asp Ile Leu Glu Asp Leu Val Ile Glu Phe 
    50                  55                  60                  


Ile Thr Glu Met Thr His Lys Ala Met Ser Ile Gly Arg Gln Gly Arg 
65                  70                  75                  80  


Val Gln Val Glu Asp Ile Val Phe Leu Ile Arg Lys Asp Pro Arg Lys 
                85                  90                  95      


Phe Ala Arg Val Lys Asp Leu Leu Thr Met Asn Glu Glu Leu Lys Arg 
            100                 105                 110         


Ala Arg Lys Ala Phe Asp Glu Ala Asn Tyr Gly Ser 
        115                 120                 


<210>  47
<211>  609
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDS 6His-TBP


<220>
<221>  CDS
<222>  (1)..(609)

<400>  47
atg ggc agc agc cat cat cat cat cat cac agc agc ggc ctg gtg ccg         48
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro           
1               5                   10                  15                

cgc ggc agc cat atg tct ggg att gta ccg cag ctg caa aat att gta         96
Arg Gly Ser His Met Ser Gly Ile Val Pro Gln Leu Gln Asn Ile Val           
            20                  25                  30                    

tcc aca gtg aat ctt ggt tgt aaa ctt gac cta aag acc att gca ctt        144
Ser Thr Val Asn Leu Gly Cys Lys Leu Asp Leu Lys Thr Ile Ala Leu           
        35                  40                  45                        

cgt gcc cga aac gcc gaa tat aat ccc aag cgg ttt gct gcg gta atc        192
Arg Ala Arg Asn Ala Glu Tyr Asn Pro Lys Arg Phe Ala Ala Val Ile           
    50                  55                  60                            

atg agg ata aga gag cca cga acc acg gca ctg att ttc agt tct ggg        240
Met Arg Ile Arg Glu Pro Arg Thr Thr Ala Leu Ile Phe Ser Ser Gly           
65                  70                  75                  80            

aaa atg gtg tgc aca gga gcc aag agt gaa gaa cag tcc aga ctg gca        288
Lys Met Val Cys Thr Gly Ala Lys Ser Glu Glu Gln Ser Arg Leu Ala           
                85                  90                  95                

gca aga aaa tat gct aga gtt gta cag aag ttg ggt ttt cca gct aag        336
Ala Arg Lys Tyr Ala Arg Val Val Gln Lys Leu Gly Phe Pro Ala Lys           
            100                 105                 110                   

ttc ttg gac ttc aag att cag aac atg gtg ggg agc tgt gat gtg aag        384
Phe Leu Asp Phe Lys Ile Gln Asn Met Val Gly Ser Cys Asp Val Lys           
        115                 120                 125                       

ttt cct ata agg tta gaa ggc ctt gtg ctc acc cac caa caa ttt agt        432
Phe Pro Ile Arg Leu Glu Gly Leu Val Leu Thr His Gln Gln Phe Ser           
    130                 135                 140                           

agt tat gag cca gag tta ttt cct ggt tta atc tac aga atg atc aaa        480
Ser Tyr Glu Pro Glu Leu Phe Pro Gly Leu Ile Tyr Arg Met Ile Lys           
145                 150                 155                 160           

ccc aga att gtt ctc ctt att ttt gtt tct gga aaa gtt gta tta aca        528
Pro Arg Ile Val Leu Leu Ile Phe Val Ser Gly Lys Val Val Leu Thr           
                165                 170                 175               

ggt gct aaa gtc aga gca gaa att tat gaa gca ttt gaa aac atc tac        576
Gly Ala Lys Val Arg Ala Glu Ile Tyr Glu Ala Phe Glu Asn Ile Tyr           
            180                 185                 190                   

cct att cta aag gga ttc agg aag acg acg taa                            609
Pro Ile Leu Lys Gly Phe Arg Lys Thr Thr                                   
        195                 200                                           


<210>  48
<211>  202
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  48

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ser Gly Ile Val Pro Gln Leu Gln Asn Ile Val 
            20                  25                  30          


Ser Thr Val Asn Leu Gly Cys Lys Leu Asp Leu Lys Thr Ile Ala Leu 
        35                  40                  45              


Arg Ala Arg Asn Ala Glu Tyr Asn Pro Lys Arg Phe Ala Ala Val Ile 
    50                  55                  60                  


Met Arg Ile Arg Glu Pro Arg Thr Thr Ala Leu Ile Phe Ser Ser Gly 
65                  70                  75                  80  


Lys Met Val Cys Thr Gly Ala Lys Ser Glu Glu Gln Ser Arg Leu Ala 
                85                  90                  95      


Ala Arg Lys Tyr Ala Arg Val Val Gln Lys Leu Gly Phe Pro Ala Lys 
            100                 105                 110         


Phe Leu Asp Phe Lys Ile Gln Asn Met Val Gly Ser Cys Asp Val Lys 
        115                 120                 125             


Phe Pro Ile Arg Leu Glu Gly Leu Val Leu Thr His Gln Gln Phe Ser 
    130                 135                 140                 


Ser Tyr Glu Pro Glu Leu Phe Pro Gly Leu Ile Tyr Arg Met Ile Lys 
145                 150                 155                 160 


Pro Arg Ile Val Leu Leu Ile Phe Val Ser Gly Lys Val Val Leu Thr 
                165                 170                 175     


Gly Ala Lys Val Arg Ala Glu Ile Tyr Glu Ala Phe Glu Asn Ile Tyr 
            180                 185                 190         


Pro Ile Leu Lys Gly Phe Arg Lys Thr Thr 
        195                 200         


<210>  49
<211>  5345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBAD-TBP-Int-CBD-12His

<400>  49
catggcctct gggattgtac cgcagctgca aaatattgta tccacagtga atcttggttg       60

taaacttgac ctaaagacca ttgcacttcg tgcccgaaac gccgaatata atcccaagcg      120

gtttgctgcg gtaatcatga ggataagaga gccacgaacc acggcactga ttttcagttc      180

tgggaaaatg gtgtgcacag gagccaagag tgaagaacag tccagactgg cagcaagaaa      240

atatgctaga gttgtacaga agttgggttt tccagctaag ttcttggact tcaagattca      300

gaacatggtg gggagctgtg atgtgaagtt tcctataagg ttagaaggcc ttgtgctcac      360

ccaccaacaa tttagtagtt atgagccaga gttatttcct ggtttaatct acagaatgat      420

caaacccaga attgttctcc ttatttttgt ttctggaaaa gttgtattaa caggtgctaa      480

agtcagagca gaaatttatg aagcatttga aaacatctac cctattctaa agggattcag      540

gaagacgacg gcctgcatca cgggagatgc actagttgcc ctacccgagg gcgagtcggt      600

acgcatcgcc gacatcgtgc cgggtgcgcg gcccaacagt gacaacgcca tcgacctgaa      660

agtccttgac cggcatggca atcccgtgct cgccgaccgg ctgttccact ccggcgagca      720

tccggtgtac acggtgcgta cggtcgaagg tctgcgtgtg acgggcaccg cgaaccaccc      780

gttgttgtgt ttggtcgacg tcgccggggt gccgaccctg ctgtggaagc tgatcgacga      840

aatcaagccg ggcgattacg cggtgattca acgcagcgca ttcagcgtcg actgtgcagg      900

ttttgcccgc ggaaaacccg aatttgcgcc cacaacctac acagtcggcg tccctggact      960

ggtgcgtttc ttggaagcac accaccgaga cccggacgcc caagctatcg ccgacgagct     1020

gaccgacggg cggttctact acgcgaaagt cgccagtgtc accgacgccg gcgtgcagcc     1080

ggtgtatagc cttcgtgtcg acacggcaga ccacgcgttt atcacgaacg ggttcgtcag     1140

ccacgctact ggcctcaccg gtctgaactc aggcctcacg acaaatcctg gtgtatccgc     1200

ttggcaggtc aacacagctt atactgcggg acaattggtc acatataacg gcaagacgta     1260

taaatgtttg cagccccaca cctccttggc aggatgggaa ccatccaacg ttcctgcctt     1320

gtggcagctt caagcgcatc atcatcatca tcatcatcac catcaccatc actgagcggc     1380

cgcactcgag agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt     1440

aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg     1500

gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg     1560

gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc     1620

gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac     1680

aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg     1740

acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct     1800

ttttgcgttt ctacaaactc tttttgttta tttttctaaa tacattcaaa tatgtatccg     1860

ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt     1920

attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt     1980

gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg     2040

ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa     2100

cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt     2160

gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag     2220

tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt     2280

gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga     2340

ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt     2400

tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta     2460

gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg     2520

caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc     2580

cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt     2640

atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg     2700

gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg     2760

attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa     2820

cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa     2880

atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga     2940

tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg     3000

ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact     3060

ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac     3120

cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg     3180

gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg     3240

gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga     3300

acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc     3360

gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg     3420

agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc     3480

tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc     3540

agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt     3600

cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc     3660

gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc     3720

ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact     3780

ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac     3840

gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg     3900

cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt     3960

gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga     4020

aggcgaagcg gcatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc     4080

tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg     4140

ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc     4200

attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg     4260

tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct     4320

cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg     4380

gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat     4440

cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt     4500

taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg     4560

aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg     4620

gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa     4680

gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag     4740

cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg     4800

gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat     4860

tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg     4920

cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg     4980

gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt     5040

gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca     5100

aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa     5160

aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg     5220

gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac     5280

gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa     5340

ttaac                                                                 5345


<210>  50
<211>  4164
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pIEx-ccdb

<400>  50
cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat       60

aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca      120

tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg      180

agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata      240

tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac      300

ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg      360

agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa      420

gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac      480

gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg      540

atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg      600

tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag      660

acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg      720

cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga      780

tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta      840

tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg      900

gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca      960

taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt     1020

tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg     1080

accatggcac accaccatca ccaccatcac caccatcact cttctggtct ggaagttctg     1140

ttccaggggc ccatggacta atgaggtacc ggatccgaat tcgagctccg tcgacaagct     1200

tgatatcgaa ttcctgcagc ccaaactgca gttgacaaca taaaaacttt gtgttatact     1260

tgtaacgtaa ggaggtaatg attcagttta aggtttacac ctataaaaga gagagccgtt     1320

atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga     1380

tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg     1440

tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct     1500

ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca     1560

ttaacctgat gttctgggga atataagctt gcggccgcac agctgtatac acgtgcaagc     1620

cagccagaac tcgccccgga agaccccgag gatctcgagc actaagtgat taacctcagg     1680

ttatacatat attttgaatt taattaatta tacatatatt ttatattatt tttgtctttt     1740

attatcgagg ggccgttgtt ggtgtggggt tttgcataga aataacaatg ggagttggcg     1800

acgttgctgc gccaacacca cctcccttcc ctcctttcat catgtatctg tagataaaat     1860

aaaatattaa acctaaaaac aagaccgcgc ctatcaacaa aatgataggc attaacttgc     1920

cgctgacgct gtcactaacg ttggacgatt tgccgactaa accttcatcg cccagtaacc     1980

aatctagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt     2040

tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca     2100

ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt     2160

ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga     2220

tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa     2280

gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct     2340

gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat     2400

acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga     2460

tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc     2520

caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat     2580

gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa     2640

cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac     2700

tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa     2760

agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc     2820

tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc     2880

ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag     2940

acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta     3000

ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa     3060

gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc     3120

gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat     3180

ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga     3240

gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt     3300

ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata     3360

cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac     3420

cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg     3480

ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg     3540

tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag     3600

cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct     3660

ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc     3720

aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt     3780

ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg     3840

tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga     3900

gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg     3960

gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg     4020

caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct     4080

tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta     4140

tgaccatgat tacgaattcc cggg                                            4164


