                         
							    PCT/EP2016/078668 - 23-02-2017




			SEQUENCE LISTING 

<110>  Novozymes A/S
 
<120>  POLYPEPTIDES HAVING PROTEASE ACTIVITY AND POLYNUCLEOTIDES 
       ENCODING SAME

<130>  13057-EP-EPA

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  1378
<212>  DNA
<213>  Beauveria brongniartii


<220>
<221>  CDS
<222>  (1)..(258)

<220>
<221>  misc_feature
<222>  (1)..(1328)
<223>  Signal peptide aa -99 to -85, Propeptide aa -84 to -1, Mature 
       peptide aa 1 to 282

<220>
<221>  sig_peptide
<222>  (1)..(45)

<220>
<221>  misc_structure
<222>  (46)..(361)
<223>  propeptide

<220>
<221>  Intron1
<222>  (259)..(322)

<220>
<221>  CDS
<222>  (323)..(514)

<220>
<221>  mat_peptide
<222>  (362)..(1328)

<220>
<221>  Intron2
<222>  (515)..(571)

<220>
<221>  CDS
<222>  (572)..(1081)

<220>
<221>  Intron3
<222>  (1082)..(1145)

<220>
<221>  CDS
<222>  (1146)..(1328)

<400>  1
atg cgt cta tca atc atc gca gcc gtt ctt ccc cta gcc att gcg gct         48
Met Arg Leu Ser Ile Ile Ala Ala Val Leu Pro Leu Ala Ile Ala Ala           
                -95                 -90                 -85               

cct gtc gtt gag cct gct cct ctc atc gag gcc agc ggg cag gcc att         96
Pro Val Val Glu Pro Ala Pro Leu Ile Glu Ala Ser Gly Gln Ala Ile           
            -80                 -75                 -70                   

gcc ggc aat tat att gtc aag ctc aag gac ggg gcg acc atg agt atc        144
Ala Gly Asn Tyr Ile Val Lys Leu Lys Asp Gly Ala Thr Met Ser Ile           
        -65                 -60                 -55                       

atg gat gcc gca tcc aag gta tcc aag ccc acg atc atc tat aca gat        192
Met Asp Ala Ala Ser Lys Val Ser Lys Pro Thr Ile Ile Tyr Thr Asp           
    -50                 -45                 -40                           

gtc ttc ccg gga tac gcg gcc agt ctc aga aaa gac gag ctt gag cgt        240
Val Phe Pro Gly Tyr Ala Ala Ser Leu Arg Lys Asp Glu Leu Glu Arg           
-35                 -30                 -25                 -20           

ctc cgc cac gac ccc aat gtaagtgcgc cttctcctca gactgtttgt               288
Leu Arg His Asp Pro Asn                                                   
                -15                                                       

gaattttact aatgagttgt gtctacgcga atag gtc gag tcg ata gag cag gat      343
                                      Val Glu Ser Ile Glu Gln Asp         
                                                  -10                     

gcc att gtt agc atc aac tcg att gtc aat caa acc gga gct ccg tgg        391
Ala Ile Val Ser Ile Asn Ser Ile Val Asn Gln Thr Gly Ala Pro Trp           
    -5              -1  1               5                   10            

ggt cta gct cgc atc tcg cac agg ccc aag gtc aac act ccg tat acc        439
Gly Leu Ala Arg Ile Ser His Arg Pro Lys Val Asn Thr Pro Tyr Thr           
                15                  20                  25                

tat cag tac gac tcg tcc gcc ggc gag ggt aca tgc gta tat gtc att        487
Tyr Gln Tyr Asp Ser Ser Ala Gly Glu Gly Thr Cys Val Tyr Val Ile           
            30                  35                  40                    

gac act ggc gtt gaa gac act cac cct gtaagcccac acatacttgg              534
Asp Thr Gly Val Glu Asp Thr His Pro                                       
        45                  50                                            

gtgcccacga aaaaagccac taattgagca tcaatag gaa ttt gaa gga cgt gcc       589
                                         Glu Phe Glu Gly Arg Ala          
                                                     55                   

aag caa gtc aaa acc ttt gtc agt ggc agt aca gat ggt cac ggc cac        637
Lys Gln Val Lys Thr Phe Val Ser Gly Ser Thr Asp Gly His Gly His           
        60                  65                  70                        

ggc aca cac tgc gcc gga acc att ggc tcc aag act ttc ggc gta gcc        685
Gly Thr His Cys Ala Gly Thr Ile Gly Ser Lys Thr Phe Gly Val Ala           
    75                  80                  85                            

aag aag acg agt gtt ttt ggc gtc aag gtg ctc gaa gac agt ggc tcg        733
Lys Lys Thr Ser Val Phe Gly Val Lys Val Leu Glu Asp Ser Gly Ser           
90                  95                  100                 105           

ggc tca ctc agc ggc gtc atc gcc gga atg gac ttt gtt gcg cag gac        781
Gly Ser Leu Ser Gly Val Ile Ala Gly Met Asp Phe Val Ala Gln Asp           
                110                 115                 120               

cgg aaa acc cgt aac tgc ccc aag ggc acc atc gcc agc atg tcc ctt        829
Arg Lys Thr Arg Asn Cys Pro Lys Gly Thr Ile Ala Ser Met Ser Leu           
            125                 130                 135                   

ggt ggt ggc tac tca gct agc gta aac cag gcc gcc gca cgt ctg cag        877
Gly Gly Gly Tyr Ser Ala Ser Val Asn Gln Ala Ala Ala Arg Leu Gln           
        140                 145                 150                       

gct tcg ggc gtt ttt gtc gcc gtc gcc gcc ggc aac gac aat cgt gat        925
Ala Ser Gly Val Phe Val Ala Val Ala Ala Gly Asn Asp Asn Arg Asp           
    155                 160                 165                           

gcc gcc aac acc tcg ccc gcc tcg gag ccg tcc gtc tgc acc gtc ggc        973
Ala Ala Asn Thr Ser Pro Ala Ser Glu Pro Ser Val Cys Thr Val Gly           
170                 175                 180                 185           

gcc acc gac tcg tcg gac cgc cgg tcc agc ttc tcc aac tat gga aaa       1021
Ala Thr Asp Ser Ser Asp Arg Arg Ser Ser Phe Ser Asn Tyr Gly Lys           
                190                 195                 200               

gtt gtt gac att ttc gca cct ggc act ggc att ctg tct acc tgg ata       1069
Val Val Asp Ile Phe Ala Pro Gly Thr Gly Ile Leu Ser Thr Trp Ile           
            205                 210                 215                   

gga ggc cgc act gtaagtttct gttcgcatca cgctgtccaa tcaaccttga           1121
Gly Gly Arg Thr                                                           
        220                                                               

agctgacttt tttttttctc ctag aat acc atc tcg ggc act tcg atg gcc        1172
                           Asn Thr Ile Ser Gly Thr Ser Met Ala            
                                       225                 230            

act ccc cac att gct ggt ctc gct gcc tac ctt ttg gtt ctc ggc aaa       1220
Thr Pro His Ile Ala Gly Leu Ala Ala Tyr Leu Leu Val Leu Gly Lys           
                235                 240                 245               

ggc act gcc gca aac ctc tgc aac gtg atc cag aat atc tct acc aag       1268
Gly Thr Ala Ala Asn Leu Cys Asn Val Ile Gln Asn Ile Ser Thr Lys           
            250                 255                 260                   

aat gtc ctg aca ggc gtt cct tca ggc acc gtc aac tac ctg gcg ttc       1316
Asn Val Leu Thr Gly Val Pro Ser Gly Thr Val Asn Tyr Leu Ala Phe           
        265                 270                 275                       

aac ggc gcc acc taagattggc tagcacgtgt ttttatgcca gtattggtcg           1368
Asn Gly Ala Thr                                                           
    280                                                                   

gtcgtcgatg                                                            1378


<210>  2
<211>  381
<212>  PRT
<213>  Beauveria brongniartii

<400>  2

Met Arg Leu Ser Ile Ile Ala Ala Val Leu Pro Leu Ala Ile Ala Ala 
                -95                 -90                 -85     


Pro Val Val Glu Pro Ala Pro Leu Ile Glu Ala Ser Gly Gln Ala Ile 
            -80                 -75                 -70         


Ala Gly Asn Tyr Ile Val Lys Leu Lys Asp Gly Ala Thr Met Ser Ile 
        -65                 -60                 -55             


Met Asp Ala Ala Ser Lys Val Ser Lys Pro Thr Ile Ile Tyr Thr Asp 
    -50                 -45                 -40                 


Val Phe Pro Gly Tyr Ala Ala Ser Leu Arg Lys Asp Glu Leu Glu Arg 
-35                 -30                 -25                 -20 


Leu Arg His Asp Pro Asn Val Glu Ser Ile Glu Gln Asp Ala Ile Val 
                -15                 -10                 -5      


Ser Ile Asn Ser Ile Val Asn Gln Thr Gly Ala Pro Trp Gly Leu Ala 
        -1  1               5                   10              


Arg Ile Ser His Arg Pro Lys Val Asn Thr Pro Tyr Thr Tyr Gln Tyr 
    15                  20                  25                  


Asp Ser Ser Ala Gly Glu Gly Thr Cys Val Tyr Val Ile Asp Thr Gly 
30                  35                  40                  45  


Val Glu Asp Thr His Pro Glu Phe Glu Gly Arg Ala Lys Gln Val Lys 
                50                  55                  60      


Thr Phe Val Ser Gly Ser Thr Asp Gly His Gly His Gly Thr His Cys 
            65                  70                  75          


Ala Gly Thr Ile Gly Ser Lys Thr Phe Gly Val Ala Lys Lys Thr Ser 
        80                  85                  90              


Val Phe Gly Val Lys Val Leu Glu Asp Ser Gly Ser Gly Ser Leu Ser 
    95                  100                 105                 


Gly Val Ile Ala Gly Met Asp Phe Val Ala Gln Asp Arg Lys Thr Arg 
110                 115                 120                 125 


Asn Cys Pro Lys Gly Thr Ile Ala Ser Met Ser Leu Gly Gly Gly Tyr 
                130                 135                 140     


Ser Ala Ser Val Asn Gln Ala Ala Ala Arg Leu Gln Ala Ser Gly Val 
            145                 150                 155         


Phe Val Ala Val Ala Ala Gly Asn Asp Asn Arg Asp Ala Ala Asn Thr 
        160                 165                 170             


Ser Pro Ala Ser Glu Pro Ser Val Cys Thr Val Gly Ala Thr Asp Ser 
    175                 180                 185                 


Ser Asp Arg Arg Ser Ser Phe Ser Asn Tyr Gly Lys Val Val Asp Ile 
190                 195                 200                 205 


Phe Ala Pro Gly Thr Gly Ile Leu Ser Thr Trp Ile Gly Gly Arg Thr 
                210                 215                 220     


Asn Thr Ile Ser Gly Thr Ser Met Ala Thr Pro His Ile Ala Gly Leu 
            225                 230                 235         


Ala Ala Tyr Leu Leu Val Leu Gly Lys Gly Thr Ala Ala Asn Leu Cys 
        240                 245                 250             


Asn Val Ile Gln Asn Ile Ser Thr Lys Asn Val Leu Thr Gly Val Pro 
    255                 260                 265                 


Ser Gly Thr Val Asn Tyr Leu Ala Phe Asn Gly Ala Thr 
270                 275                 280         


<210>  3
<211>  269
<212>  PRT
<213>  Bacillus lentus

<400>  3

Ala Gln Ser Val Pro Trp Gly Ile Arg Arg Val Gln Ala Pro Thr Ala 
1               5                   10                  15      


His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Glu Asn Gly His Gly Thr 
    50                  55                  60                  


His Ala Ala Gly Thr Ile Ala Ala Leu Asp Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 
        115                 120                 125             


Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 
        195                 200                 205             


Ala Ser Leu Asp Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Arg Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 
                245                 250                 255     


Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  4
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  Beauveria brongniartii  specific primer

<400>  4
acacaactgg ggatccacca tgcgtctatc aatcatcgca gc                          42


<210>  5
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Beauveria brongniartii  specific primer

<400>  5
ccctctagat ctcgagcatc gacgaccgac caatactg                               38




                              RECTIFIED SHEET (R91) ISA/EP