                         SEQUENCE LISTING

<110>  Henkel AG & Co. KGaA
 
<120>  Proteasevarianten mit verbesserter Enzymstabilitt in Wasch- und 
       Reinigungsmitteln

<130>  PT033299PCT

<150>  DE102015223270.0
<151>  2015-11-25

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  269
<212>  PRT
<213>  Bacillus pumilus (Wildtyp)

<400>  1

Ala Gln Thr Ile Pro Trp Gly Ile Asn Arg Val Gln Ala Pro Ile Ala 
1               5                   10                  15      


Gln Ser Arg Gly Phe Thr Gly Thr Gly Val Arg Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Asn His Ala Asp Leu Arg Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Asn Ile Ser Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Asn Val Asp Leu Tyr Gly Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Arg Gly Ser Ile Ser Gly Ile Ala Gln Gly Leu Gln Trp Ala 
            100                 105                 110         


Ala Asn Asn Gly Met His Ile Ala Asn Met Ser Leu Gly Ser Ser Ala 
        115                 120                 125             


Gly Ser Ala Thr Met Glu Gln Ala Val Asn Gln Ala Thr Ala Ser Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Asn Val Gly 
145                 150                 155                 160 


Phe Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Val Gln Ser Thr Val Pro Gly Asn Gly Tyr 
        195                 200                 205             


Ala Ser Phe Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Thr Thr Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  2
<211>  269
<212>  PRT
<213>  Bacillus pumilus (Mutante 1)

<400>  2

Ala Gln Thr Ile Pro Trp Gly Ile Asn Arg Val Gln Ala Pro Ile Ala 
1               5                   10                  15      


Gln Ser Arg Gly Phe Thr Gly Thr Gly Val Arg Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Asn His Ala Asp Leu Arg Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Asn Ile Ser Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Asn Val Asp Leu Tyr Gly Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Arg Gly Ser Ile Ser Gly Ile Ala Gln Gly Leu Gln Trp Ala 
            100                 105                 110         


Ala Asn Asn Gly Met His Ile Ala Asn Met Ser Leu Gly Ser Ser Ala 
        115                 120                 125             


Gly Ser Ala Thr Met Glu Gln Ala Val Asn Gln Ala Thr Ala Ser Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Asn Val Gly 
145                 150                 155                 160 


Phe Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Val Gln Ser Thr Val Pro Gly Asp Gly Tyr 
        195                 200                 205             


Ala Ser Phe Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Thr Thr Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  3
<211>  269
<212>  PRT
<213>  Bacillus pumilus (Mutante 2)

<400>  3

Ala Gln Thr Ile Pro Trp Gly Ile Asn Arg Val Gln Ala Pro Ile Ala 
1               5                   10                  15      


Gln Ser Arg Gly Phe Thr Gly Ser Gly Val Arg Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Asn His Ala Asp Leu Arg Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Asn Ile Ser Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Asn Val Asp Leu Tyr Gly Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Arg Gly Ser Ile Ser Gly Ile Ala Gln Gly Leu Gln Trp Ala 
            100                 105                 110         


Ala Asn Asn Gly Met His Ile Ala Asn Met Ser Leu Gly Ser Ser Ala 
        115                 120                 125             


Gly Ser Ala Thr Met Glu Gln Ala Val Asn Gln Ala Thr Ala Ser Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Asn Val Gly 
145                 150                 155                 160 


Phe Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Val Gln Ser Thr Val Pro Gly Asn Gly Tyr 
        195                 200                 205             


Ala Ser Phe Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Thr Thr Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  4
<211>  269
<212>  PRT
<213>  Bacillus pumilus (Mutante 3)

<400>  4

Ala Gln Thr Ile Pro Trp Gly Ile Asn Arg Val Gln Ala Pro Ile Ala 
1               5                   10                  15      


Gln Ser Arg Gly Phe Thr Gly Thr Gly Val Arg Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Asn His Ala Asp Leu Arg Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Asn Ile Ser Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Asn Val Asp Leu Tyr Gly Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Arg Gly Ser Ile Ser Gly Ile Ala Gln Gly Leu Gln Trp Ala 
            100                 105                 110         


Ala Asn Asn Gly Met His Ile Ala Asn Met Ser Leu Gly Ser Ser Ala 
        115                 120                 125             


Gly Ser Ala Thr Met Glu Gln Ala Val Asn Gln Ala Thr Ala Ser Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Glu Asn Val Gly 
145                 150                 155                 160 


Phe Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp His 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Val Gln Ser Thr Val Pro Gly Asn Gly Tyr 
        195                 200                 205             


Ala Ser Phe Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Thr Thr Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  5
<211>  269
<212>  PRT
<213>  Bacillus pumilus (Mutante 4)

<400>  5

Ala Gln Thr Ile Pro Trp Gly Ile Asn Arg Val Gln Ala Pro Ile Ala 
1               5                   10                  15      


His Ser Arg Gly Phe Thr Gly Thr Gly Val Arg Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Asn His Ala Asp Leu Arg Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Asn Ile Ser Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Asn Val Asp Leu Tyr Gly Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Arg Gly Ser Ile Ser Gly Ile Ala Gln Gly Leu Gln Trp Ala 
            100                 105                 110         


Ala Asn Asn Gly Met His Ile Ala Asn Met Ser Leu Gly Ser Ser Ala 
        115                 120                 125             


Gly Ser Ala Thr Met Glu Gln Ala Val Asn Gln Ala Thr Ala Ser Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Asn Val Gly 
145                 150                 155                 160 


Phe Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Val Gln Ser Thr Val Pro Gly Asn Gly Tyr 
        195                 200                 205             


Ala Ser Phe Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Thr Thr Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  6
<211>  269
<212>  PRT
<213>  Bacillus pumilus (Mutante 5)

<400>  6

Ala Gln Thr Ile Pro Trp Gly Ile Asn Arg Val Gln Ala Pro Ile Ala 
1               5                   10                  15      


Gln Ser Arg Gly Phe Thr Gly Thr Gly Val Arg Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Asn His Ala Asp Leu Arg Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Asn Ile Ser Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Asn Val Asp Leu Tyr Gly Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Arg Gly Ser Ile Ser Gly Ile Ala Gln Gly Leu Gln Trp Ala 
            100                 105                 110         


Ala Asn Asn Gly Met His Ile Ala Asn Met Ser Leu Gly Ser Ser Ala 
        115                 120                 125             


Gly Ser Ala Thr Met Glu Gln Ala Val Asn His Ala Thr Ala Ser Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Glu Asn Val Gly 
145                 150                 155                 160 


Phe Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Val Gln Ser Thr Val Pro Gly Asn Gly Tyr 
        195                 200                 205             


Ala Ser Phe Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Thr Thr Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  7
<211>  269
<212>  PRT
<213>  Bacillus pumilus (Mutante 6)

<400>  7

Ala Gln Thr Ile Pro Trp Gly Ile Asn Arg Val Gln Ala Pro Ile Ala 
1               5                   10                  15      


Gln Ser Arg Gly Phe Thr Gly Thr Gly Val Arg Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Asn His Ala Asp Leu Arg Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Asn Ile Ser Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Asn Val Asp Leu Tyr Gly Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Arg Gly Ser Ile Ser Gly Ile Ala Gln Gly Leu Gln Trp Ala 
            100                 105                 110         


Ala Asn Asn Gly Met His Ile Ala Asn Met Ser Leu Gly Ser Ser Ala 
        115                 120                 125             


Gly Ser Ala Thr Met Glu Gln Ala Val Asn Gln Ala Thr Ala Ser Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Glu Gly Asn Val Gly 
145                 150                 155                 160 


Phe Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Val Gln Ser Thr Val Pro Gly Asn Gly Tyr 
        195                 200                 205             


Ala Ser Phe Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Thr Thr Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  8
<211>  269
<212>  PRT
<213>  Bacillus pumilus (Mutante 7)

<400>  8

Ala Gln Thr Ile Pro Trp Gly Ile Asn Arg Val Gln Ala Pro Ile Ala 
1               5                   10                  15      


Gln Ser Arg Gly Phe Thr Gly Thr Gly Val Arg Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Asn His Ala Asp Leu Arg Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Asn Ile Ser Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Asp Val Asp Leu Tyr Gly Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Arg Gly Ser Ile Ser Gly Ile Ala Gln Gly Leu Gln Trp Ala 
            100                 105                 110         


Ala Asn Asn Gly Met His Ile Ala Asn Met Ser Leu Gly Ser Ser Ala 
        115                 120                 125             


Gly Ser Ala Thr Met Glu Gln Ala Val Asn Gln Ala Thr Ala Ser Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Asp Val Gly 
145                 150                 155                 160 


Phe Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Lys Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Val Gln Ser Thr Val Pro Gly Asn Gly Tyr 
        195                 200                 205             


Ala Ser Phe Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Thr Thr Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  9
<211>  810
<212>  DNA
<213>  Bacillus pumilus (Wildtyp)

<400>  9
gcgcaaacaa tcccatgggg cattaaccgt gtacaagctc cgattgccca aagcagagga       60

ttcacaggta ctggagttcg tgttgctgtc ttagacacag ggatctcaaa tcacgctgat      120

ttaagaattc gtggcggtgc gagttttgta ccaggagagc cgaacattag tgatggaaac      180

ggtcatggta cccacgttgc tggtacaatt gcagcgttaa acaattcaat cggtgtactt      240

ggcgtagcac ctaacgttga tttatatggg gttaaagtgc taggagcaag tggcagaggg      300

tcaatcagtg gtattgcaca aggtttacaa tgggctgcaa ataatggcat gcatattgct      360

aacatgagtt taggaagtag tgctggatct gctacaatgg aacaagctgt taaccaagca      420

acagcaagtg gcgttcttgt agtcgcagct tctggtaact caggtgcagg aaatgttgga      480

ttcccagcac gctatgcaaa tgcgatggct gttggtgcaa cagatcaaaa caacaaccgc      540

gctagctttt ctcagtacgg agcaggtctt gacattgtcg caccaggtgt aggtgtacaa      600

agtacggttc ctggcaatgg atacgcaagc ttcaatggta catctatggc tacaccgcac      660

gttgctggtg ttgctgcgtt agtgaagcaa aagaatcctt cttggtcaaa tgttcaaatt      720

cgtaaccacc ttaaaaatac ggcaacaaac ttaggaaata cgactcaatt cggaagtggt      780

cttgttaacg cagaagcggc aacacgttaa                                       810


