﻿               SEQUENCE LISTING

<110> Eberhard-Karls-Universität Tübingen Medizinische Fakultät

<120> New Tumor Marker

<130> 5402P555WO

<160> 11

<170> BiSSAP 1.3

<210> 1
<211> 1586
<212> DNA
<213> Homo sapiens

<220> 
<223> /note="P2RX7 Splice Variant"
      /note="P2X7 Splice-Variant"

<400> 1
aaaacgcagg gagggaggct gtcaccatgc cggcctgctg cagctgcagt gatgttttcc      60

agtatgagac gaacaaagtc actcggatcc agagcatgaa ttatggcacc attaagtggt     120

tcttccacgt gatcatcttt tcctacgttt gctttgctct ggtgagtgac aagctgtacc     180

agcggaaaga gcctgtcatc agttctgtgc acaccaaggt gaaggggata gcagaggtga     240

aagaggagat cgtggagaat ggagtgaaga agttggtgca cagtgtcttt gacaccgcag     300

actacacctt ccctttgcag gggaactctt tcttcgtgat gacaaacttt ctcaaaacag     360

aaggccaaga gcagcggttg tgtcccgagt atcccacccg caggacgctc tgttcctctg     420

accgaggttg taaaaaggga tggatggacc cgcagagcaa aggaattcag accggaaggt     480

gtgtagtgta tgaagggaac cagaagacct gtgaagtctc tgcctggtgc cccatcgagg     540

cagtggaaga ggccccccgg cctgctctct tgaacagtgc cgaaaacttc actgtgctca     600

tcaagaacaa tatcgacttc cccggccaca actacaccac atacgccaag tactacaagg     660

aaaacaatgt tgagaaacgg actctgataa aagtcttcgg gatccgtttt gacatcctgg     720

tttttggcac cggaggaaaa tttgacatta tccagctggt tgtgtacatc ggctcaaccc     780

tctcctactt cggtctggcc gctgtgttca tcgacttcct catcgacact tactccagta     840

actgctgtcg ctcccatatt tatccctggt gcaagtgctg tcagccctgt gtggtcaacg     900

aatactacta caggaagaag tgcgagtcca ttgtggagcc aaagccgaca ttaaagtatg     960

tgtcctttgt ggatgaatcc cacattagga tggtgaacca gcagctacta gggagaagtc    1020

tgcaagatgt caagggccaa gaagtcccaa gacctgcgat ggacttcaca gatttgtcca    1080

ggctgcccct ggccctccat gacacacccc cgattcctgg acaaccagag gagatacagc    1140

tgcttagaaa ggaggcgact cctagatcca gggatagccc cgtctggtgc cagtgtggaa    1200

gctgcctccc atctcaactc cctgagagcc acaggtgcct ggaggagctg tgctgccgga    1260

aaaagccggg ggcctgcatc accacctcag agctgttcag gaagctggtc ctgtccagac    1320

acgtcctgca gttcctcctg ctctaccagg agcccttgct ggcgctggat gtggattcca    1380

ccaacagccg gctgcggcac tgtgcctaca ggtgctacgc cacctggcgc ttcggctccc    1440

aggacatggc tgactttgcc atcctgccca gctgctgccg ctggaggatc cggaaagagt    1500

ttccgaagag tgaagggcag tacagtggct tcaagagtcc ttactgaagc caggcaccgt    1560

ggctcacgtc tgtaatccca cctttt                                         1586


<210> 2
<211> 506
<212> PRT
<213> Homo sapiens

<220> 
<223> P2RX7 Splice Variant

<400> 2
Met Pro Ala Cys Cys Ser Cys Ser Asp Val Phe Gln Tyr Glu Thr Asn 
1               5                   10                  15      
Lys Val Thr Arg Ile Gln Ser Met Asn Tyr Gly Thr Ile Lys Trp Phe 
            20                  25                  30          
Phe His Val Ile Ile Phe Ser Tyr Val Cys Phe Ala Leu Val Ser Asp 
        35                  40                  45              
Lys Leu Tyr Gln Arg Lys Glu Pro Val Ile Ser Ser Val His Thr Lys 
    50                  55                  60                  
Val Lys Gly Ile Ala Glu Val Lys Glu Glu Ile Val Glu Asn Gly Val 
65                  70                  75                  80  
Lys Lys Leu Val His Ser Val Phe Asp Thr Ala Asp Tyr Thr Phe Pro 
                85                  90                  95      
Leu Gln Gly Asn Ser Phe Phe Val Met Thr Asn Phe Leu Lys Thr Glu 
            100                 105                 110         
Gly Gln Glu Gln Arg Leu Cys Pro Glu Tyr Pro Thr Arg Arg Thr Leu 
        115                 120                 125             
Cys Ser Ser Asp Arg Gly Cys Lys Lys Gly Trp Met Asp Pro Gln Ser 
    130                 135                 140                 
Lys Gly Ile Gln Thr Gly Arg Cys Val Val Tyr Glu Gly Asn Gln Lys 
145                 150                 155                 160 
Thr Cys Glu Val Ser Ala Trp Cys Pro Ile Glu Ala Val Glu Glu Ala 
                165                 170                 175     
Pro Arg Pro Ala Leu Leu Asn Ser Ala Glu Asn Phe Thr Val Leu Ile 
            180                 185                 190         
Lys Asn Asn Ile Asp Phe Pro Gly His Asn Tyr Thr Thr Tyr Ala Lys 
        195                 200                 205             
Tyr Tyr Lys Glu Asn Asn Val Glu Lys Arg Thr Leu Ile Lys Val Phe 
    210                 215                 220                 
Gly Ile Arg Phe Asp Ile Leu Val Phe Gly Thr Gly Gly Lys Phe Asp 
225                 230                 235                 240 
Ile Ile Gln Leu Val Val Tyr Ile Gly Ser Thr Leu Ser Tyr Phe Gly 
                245                 250                 255     
Leu Ala Ala Val Phe Ile Asp Phe Leu Ile Asp Thr Tyr Ser Ser Asn 
            260                 265                 270         
Cys Cys Arg Ser His Ile Tyr Pro Trp Cys Lys Cys Cys Gln Pro Cys 
        275                 280                 285             
Val Val Asn Glu Tyr Tyr Tyr Arg Lys Lys Cys Glu Ser Ile Val Glu 
    290                 295                 300                 
Pro Lys Pro Thr Leu Lys Tyr Val Ser Phe Val Asp Glu Ser His Ile 
305                 310                 315                 320 
Arg Met Val Asn Gln Gln Leu Leu Gly Arg Ser Leu Gln Asp Val Lys 
                325                 330                 335     
Gly Gln Glu Val Pro Arg Pro Ala Met Asp Phe Thr Asp Leu Ser Arg 
            340                 345                 350         
Leu Pro Leu Ala Leu His Asp Thr Pro Pro Ile Pro Gly Gln Pro Glu 
        355                 360                 365             
Glu Ile Gln Leu Leu Arg Lys Glu Ala Thr Pro Arg Ser Arg Asp Ser 
    370                 375                 380                 
Pro Val Trp Cys Gln Cys Gly Ser Cys Leu Pro Ser Gln Leu Pro Glu 
385                 390                 395                 400 
Ser His Arg Cys Leu Glu Glu Leu Cys Cys Arg Lys Lys Pro Gly Ala 
                405                 410                 415     
Cys Ile Thr Thr Ser Glu Leu Phe Arg Lys Leu Val Leu Ser Arg His 
            420                 425                 430         
Val Leu Gln Phe Leu Leu Leu Tyr Gln Glu Pro Leu Leu Ala Leu Asp 
        435                 440                 445             
Val Asp Ser Thr Asn Ser Arg Leu Arg His Cys Ala Tyr Arg Cys Tyr 
    450                 455                 460                 
Ala Thr Trp Arg Phe Gly Ser Gln Asp Met Ala Asp Phe Ala Ile Leu 
465                 470                 475                 480 
Pro Ser Cys Cys Arg Trp Arg Ile Arg Lys Glu Phe Pro Lys Ser Glu 
                485                 490                 495     
Gly Gln Tyr Ser Gly Phe Lys Ser Pro Tyr 
            500                 505     

<210> 3
<211> 1853
<212> DNA
<213> Homo sapiens

<220> 
<223> P2RX7 Wild Type

<400> 3
aaaacgcagg gagggaggct gtcaccatgc cggcctgctg cagctgcagt gatgttttcc      60

agtatgagac gaacaaagtc actcggatcc agagcatgaa ttatggcacc attaagtggt     120

tcttccacgt gatcatcttt tcctacgttt gctttgctct ggtgagtgac aagctgtacc     180

agcggaaaga gcctgtcatc agttctgtgc acaccaaggt gaaggggata gcagaggtga     240

aagaggagat cgtggagaat ggagtgaaga agttggtgca cagtgtcttt gacaccgcag     300

actacacctt ccctttgcag gggaactctt tcttcgtgat gacaaacttt ctcaaaacag     360

aaggccaaga gcagcggttg tgtcccgagt atcccacccg caggacgctc tgttcctctg     420

accgaggttg taaaaaggga tggatggacc cgcagagcaa aggaattcag accggaaggt     480

gtgtagtgta tgaagggaac cagaagacct gtgaagtctc tgcctggtgc cccatcgagg     540

cagtggaaga ggccccccgg cctgctctct tgaacagtgc cgaaaacttc actgtgctca     600

tcaagaacaa tatcgacttc cccggccaca actacaccac gagaaacatc ctgccaggtt     660

taaacatcac ttgtaccttc cacaagactc agaatccaca gtgtcccatt ttccgactag     720

gagacatctt ccgagaaaca ggcgataatt tttcagatgt ggcaattcag ggcggaataa     780

tgggcattga gatctactgg gactgcaacc tagaccgttg gttccatcac tgccatccca     840

aatacagttt ccgtcgcctt gacgacaaga ccaccaacgt gtccttgtac cctggctaca     900

acttcagata cgccaagtac tacaaggaaa acaatgttga gaaacggact ctgataaaag     960

tcttcgggat ccgttttgac atcctggttt ttggcaccgg aggaaaattt gacattatcc    1020

agctggttgt gtacatcggc tcaaccctct cctacttcgg tctggccgct gtgttcatcg    1080

acttcctcat cgacacttac tccagtaact gctgtcgctc ccatatttat ccctggtgca    1140

agtgctgtca gccctgtgtg gtcaacgaat actactacag gaagaagtgc gagtccattg    1200

tggagccaaa gccgacatta aagtatgtgt cctttgtgga tgaatcccac attaggatgg    1260

tgaaccagca gctactaggg agaagtctgc aagatgtcaa gggccaagaa gtcccaagac    1320

ctgcgatgga cttcacagat ttgtccaggc tgcccctggc cctccatgac acacccccga    1380

ttcctggaca accagaggag atacagctgc ttagaaagga ggcgactcct agatccaggg    1440

atagccccgt ctggtgccag tgtggaagct gcctcccatc tcaactccct gagagccaca    1500

ggtgcctgga ggagctgtgc tgccggaaaa agccgggggc ctgcatcacc acctcagagc    1560

tgttcaggaa gctggtcctg tccagacacg tcctgcagtt cctcctgctc taccaggagc    1620

ccttgctggc gctggatgtg gattccacca acagccggct gcggcactgt gcctacaggt    1680

gctacgccac ctggcgcttc ggctcccagg acatggctga ctttgccatc ctgcccagct    1740

gctgccgctg gaggatccgg aaagagtttc cgaagagtga agggcagtac agtggcttca    1800

agagtcctta ctgaagccag gcaccgtggc tcacgtctgt aatcccacct ttt           1853


<210> 4
<211> 267
<212> DNA
<213> Homo sapiens

<220> 
<223> /note="Gap"
      /note="Gap"

<400> 4
gagaaacatc ctgccaggtt taaacatcac ttgtaccttc cacaagactc agaatccaca     60

gtgtcccatt ttccgactag gagacatctt ccgagaaaca ggcgataatt tttcagatgt    120

ggcaattcag ggcggaataa tgggcattga gatctactgg gactgcaacc tagaccgttg    180

gttccatcac tgccatccca aatacagttt ccgtcgcctt gacgacaaga ccaccaacgt    240

gtccttgtac cctggctaca acttcag                                        267


<210> 5
<211> 595
<212> PRT
<213> Homo sapiens

<220> 
<223> P2RX7 Wild Type

<400> 5
Met Pro Ala Cys Cys Ser Cys Ser Asp Val Phe Gln Tyr Glu Thr Asn 
1               5                   10                  15      
Lys Val Thr Arg Ile Gln Ser Met Asn Tyr Gly Thr Ile Lys Trp Phe 
            20                  25                  30          
Phe His Val Ile Ile Phe Ser Tyr Val Cys Phe Ala Leu Val Ser Asp 
        35                  40                  45              
Lys Leu Tyr Gln Arg Lys Glu Pro Val Ile Ser Ser Val His Thr Lys 
    50                  55                  60                  
Val Lys Gly Ile Ala Glu Val Lys Glu Glu Ile Val Glu Asn Gly Val 
65                  70                  75                  80  
Lys Lys Leu Val His Ser Val Phe Asp Thr Ala Asp Tyr Thr Phe Pro 
                85                  90                  95      
Leu Gln Gly Asn Ser Phe Phe Val Met Thr Asn Phe Leu Lys Thr Glu 
            100                 105                 110         
Gly Gln Glu Gln Arg Leu Cys Pro Glu Tyr Pro Thr Arg Arg Thr Leu 
        115                 120                 125             
Cys Ser Ser Asp Arg Gly Cys Lys Lys Gly Trp Met Asp Pro Gln Ser 
    130                 135                 140                 
Lys Gly Ile Gln Thr Gly Arg Cys Val Val Tyr Glu Gly Asn Gln Lys 
145                 150                 155                 160 
Thr Cys Glu Val Ser Ala Trp Cys Pro Ile Glu Ala Val Glu Glu Ala 
                165                 170                 175     
Pro Arg Pro Ala Leu Leu Asn Ser Ala Glu Asn Phe Thr Val Leu Ile 
            180                 185                 190         
Lys Asn Asn Ile Asp Phe Pro Gly His Asn Tyr Thr Thr Arg Asn Ile 
        195                 200                 205             
Leu Pro Gly Leu Asn Ile Thr Cys Thr Phe His Lys Thr Gln Asn Pro 
    210                 215                 220                 
Gln Cys Pro Ile Phe Arg Leu Gly Asp Ile Phe Arg Glu Thr Gly Asp 
225                 230                 235                 240 
Asn Phe Ser Asp Val Ala Ile Gln Gly Gly Ile Met Gly Ile Glu Ile 
                245                 250                 255     
Tyr Trp Asp Cys Asn Leu Asp Arg Trp Phe His His Cys Arg Pro Lys 
            260                 265                 270         
Tyr Ser Phe Arg Arg Leu Asp Asp Lys Thr Thr Asn Val Ser Leu Tyr 
        275                 280                 285             
Pro Gly Tyr Asn Phe Arg Tyr Ala Lys Tyr Tyr Lys Glu Asn Asn Val 
    290                 295                 300                 
Glu Lys Arg Thr Leu Ile Lys Val Phe Gly Ile Arg Phe Asp Ile Leu 
305                 310                 315                 320 
Val Phe Gly Thr Gly Gly Lys Phe Asp Ile Ile Gln Leu Val Val Tyr 
                325                 330                 335     
Ile Gly Ser Thr Leu Ser Tyr Phe Gly Leu Ala Ala Val Phe Ile Asp 
            340                 345                 350         
Phe Leu Ile Asp Thr Tyr Ser Ser Asn Cys Cys Arg Ser His Ile Tyr 
        355                 360                 365             
Pro Trp Cys Lys Cys Cys Gln Pro Cys Val Val Asn Glu Tyr Tyr Tyr 
    370                 375                 380                 
Arg Lys Lys Cys Glu Ser Ile Val Glu Pro Lys Pro Thr Leu Lys Tyr 
385                 390                 395                 400 
Val Ser Phe Val Asp Glu Ser His Ile Arg Met Val Asn Gln Gln Leu 
                405                 410                 415     
Leu Gly Arg Ser Leu Gln Asp Val Lys Gly Gln Glu Val Pro Arg Pro 
            420                 425                 430         
Ala Met Asp Phe Thr Asp Leu Ser Arg Leu Pro Leu Ala Leu His Asp 
        435                 440                 445             
Thr Pro Pro Ile Pro Gly Gln Pro Glu Glu Ile Gln Leu Leu Arg Lys 
    450                 455                 460                 
Glu Ala Thr Pro Arg Ser Arg Asp Ser Pro Val Trp Cys Gln Cys Gly 
465                 470                 475                 480 
Ser Cys Leu Pro Ser Gln Leu Pro Glu Ser His Arg Cys Leu Glu Glu 
                485                 490                 495     
Leu Cys Cys Arg Lys Lys Pro Gly Ala Cys Ile Thr Thr Ser Glu Leu 
            500                 505                 510         
Phe Arg Lys Leu Val Leu Ser Arg His Val Leu Gln Phe Leu Leu Leu 
        515                 520                 525             
Tyr Gln Glu Pro Leu Leu Ala Leu Asp Val Asp Ser Thr Asn Ser Arg 
    530                 535                 540                 
Leu Arg His Cys Ala Tyr Arg Cys Tyr Ala Thr Trp Arg Phe Gly Ser 
545                 550                 555                 560 
Gln Asp Met Ala Asp Phe Ala Ile Leu Pro Ser Cys Cys Arg Trp Arg 
                565                 570                 575     
Ile Arg Lys Glu Phe Pro Lys Ser Glu Gly Gln Tyr Ser Gly Phe Lys 
            580                 585                 590         
Ser Pro Tyr 
        595 

<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> /note="combined DNA/RNA molecule"
      /note="P2XR7, fw-Primer"

<400> 6
ggtttttggc accggaggaa                                                20


<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> /note="combined DNA/RNA molecule"
      /note="P2XR7, rev-Primer"

<400> 7
ccactgtact gcccttcact                                                20


<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> /note="combined DNA/RNA molecule"
      /note="GAPDH, fw-Primer"

<400> 8
ctctgctcct cctgttcgac                                                20


<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> /note="combined DNA/RNA molecule"
      /note="GADPH, rev-Primer"

<400> 9
ttaaaagcag ccctggtgac                                                20


<210> 10
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> /note="combined DNA/RNA molecule"
      /note="TBP, fw-Primer"

<400> 10
gcccgaaacg ccgaatat                                                  18


<210> 11
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> /note="combined DNA/RNA molecule"
      /note="TBP, rev-Primer"

<400> 11
ccgtggttcg tggctctc                                                  18


