                         SEQUENCE LISTING

<110>  Virttu Biologics Limited
 
<120>  Herpes Simplex Viruses

<130>  RIC/MP7234370

<150>  GB1520345.8
<151>  2015-11-18

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  1620
<212>  DNA
<213>  Homo sapiens

<400>  1
agcccgggcc ccccgcgggc gcgcgcgcgc gcaaaaaagg cgggcggcgg tccgggcggc       60

gtgcgcgcgc gcggcgggcg tggggggcgg ggccgcggga gcggggggag gagcgggggg      120

aggagcgggg ggaggagcgg ggggaggagc ggggggagga gcggggggag gagcgggggg      180

aggagcgggg ggaggagcgg ggggaggagc ggggggagga gcggggggag gagcgggggg      240

aggagcgggg ggaggagcgg ggggaggagc ggggggagga gcggggggag gagcgggggg      300

aggagcgggg ggaggagcgg ccagacgccg aaaacgggcc ccccccaaaa cacacccccc      360

gggggtcgcg cgcggccctt taaagcggtg gcggcgggca gcccgggccc cccgcggccg      420

agactagcga gttagacagg caagcactac tcgcctctgc acgcacatgc ttgcctgtca      480

aactctacca ccccggcacg ctctctgtct ccatggcccg ccgccgccgc catcgcggcc      540

cccgccgccc ccggccgccc gggcccacgg gcgccgtccc aaccgcacag tcccaggtaa      600

cctccacgcc caactcggaa cccgcggtca ggagcgcgcc cgcggccgcc ccgccgccgc      660

cccccgccgg tgggcccccg ccttcttgtt cgctgctgct gcgccagtgg ctccacgttc      720

ccgagtccgc gtccgacgac gacgatgacg acgactggcc ggacagcccc ccgcccgagc      780

cggcgccaga ggcccggccc accgccgccg ccccccggcc ccggccccca ccgcccggcg      840

tgggcccggg gggcggggct gacccctccc accccccctc gcgccccttc cgccttccgc      900

cgcgcctcgc cctccgcctg cgcgtcaccg cggagcacct ggcgcgcctg cgcctgcgac      960

gcgcgggcgg ggagggggcg ccggagcccc ccgcgacccc cgcgaccccc gcgacccccg     1020

cgacccccgc gacccccgcg cgggtgcgct tctcgcccca cgtccgggtg cgccacctgg     1080

tggtctgggc ctcggccgcc cgcctggcgc gccgcggctc gtgggcccgc gagcgggccg     1140

accgggctcg gttccggcgc cgggtggcgg aggccgaggc ggtcatcggg ccgtgcctgg     1200

ggcccgaggc ccgtgcccgg gccctggccc gcggagccgg cccggcgaac tcggtctaac     1260

gttacacccg aggcggcctg ggtcttccgc ggagctcccg ggagctccgc accaagccgc     1320

tctccggaga gacgatggca ggagccgcgc atatatacgc ttggagccag cccgccctca     1380

cagggcgggc cgcctcgggg gcgggactgg ccaatcggcg gccgccagcg cggcggggcc     1440

cggccaacca gcgtccgccg agtcttcggg gcccggccca ttgggcggga gttaccgccc     1500

aatgggccgg gccgcccact tcccggtatg gtaattaaaa acttgcaaga ggccttgttc     1560

cgcttcccgg tatggtaatt agaaactcat taatgggcgg ccccggccgc ccttcccgct     1620


<210>  2
<211>  1800
<212>  DNA
<213>  Homo sapiens

<400>  2
agtggttacg cgcggcgcgg gaggcgggga ataccggggt tgcccattaa gggccgcggg       60

aattgccgga agcgggaagg gcggccgggg ccgcccatta atgagtttct aattaccata      120

ccgggaagcg gaacaaggcc tcttgcaagt ttttaattac cataccggga agtgggcggc      180

ccggcccatt gggcggtaac tcccgcccaa tgggccgggc cccgaagact cggcggacgc      240

tggttggccg ggccccgccg cgctggcggc cgccgattgg ccagtcccgc ccccgaggcg      300

gcccgccctg tgagggcggg ctggctccaa gcgtatatat gcgcggctcc tgccatcgtc      360

tctccggaga gcggcttggt gcggagctcc cgggagctcc gcggaagacc caggccgcct      420

cgggtgtaac gttagaccga gttcgccggg ccggctccgc gggccagggc ccgggcacgg      480

gcctcgggcc ccaggcacgg cccgatgacc gcctcggcct ccgccacccg gcgccggaac      540

cgagcccggt cggcccgctc gcgggcccac gagccgcggc gcgccaggcg ggcggccgag      600

gcccagacca ccaggtggcg cacccggacg tggggcgaga agcgcacccg cgcgggggtc      660

gcgggggtcg cgggggtcgc gggggtcgcg ggggtcgcgg ggggctccgg cgccccctcc      720

ccgcccgcgc gtcgcaggcg caggcgcgcc aggtgctccg cggtgacgcg caggcggagg      780

gcgaggcgcg gcggaaggcg gaaggggcgc gagggggggt gggaggggtc agccccgccc      840

cccgggccca cgccgggcgg tgggggccgg ggccgggggg cggcggcggt gggccgggcc      900

tctggcgccg gctcgggcgg ggggctgtcc ggccagtcgt cgtcatcgtc gtcgtcggac      960

gcggactcgg gaacgtggag ccactggcgc agcagcagcg aacaagaagg cgggggccca     1020

ccggcggggg gcggcggcgg ggcggccgcg ggcgcgctcc tgaccgcggg ttccgagttg     1080

ggcgtggagg ttacctggga ctgtgcggtt gggacggcgc ccgtgggccc gggcggccgg     1140

gggcggcggg ggccgcgatg gcggcggcgg cgggccatgg agacagagag cgtgccgggg     1200

tggtagagtt tgacaggcaa gcatgtgcgt gcagaggcga gtagtgcttg cctgtctaac     1260

tcgctagtct cggccgcggg gggcccgggc tgcccgccgc caccgcttta aagggccgcg     1320

cgcgaccccc ggggggtgtg ttttgggggg ggcccgtttt cggcgtctgg ccgctcctcc     1380

ccccgctcct ccccccgctc ctccccccgc tcctcccccc gctcctcccc ccgctcctcc     1440

ccccgctcct ccccccgctc ctccccccgc tcctcccccc gctcctcccc ccgctcctcc     1500

ccccgctcct ccccccgctc ctccccccgc tcctcccccc gctcctcccc ccgctcctcc     1560

ccccgctcct ccccccgctc ctccccccgc tcccgcggcc ccgcccccca cgcccgccgc     1620

gcgcgcgcac gccgcccgga ccgccgcccg ccttttttgc gcgcgcgcgc gcccgcgggg     1680

ggcccgggct gccacaggtg aaaccaacag agcacggcgc actccgcacg tcacacgtca     1740

cgtcatccac cacacctgcc caacaacaca actcacagcg acaactcacc gcgcaacaac     1800


<210>  3
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human herpesvirus 1 from start codon to BstEII site

<400>  3
atggcccgcc gccgccgcca tcgcggcccc cgccgccccc ggccgcccgg gcccacgggc       60

gccgtcccaa ccgcacagtc ccaggtaacc                                        90


<210>  4
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human Herpesvirus 1 from BtgZI cut site to BsTEII cut site

<400>  4
cccgccgccg ccgccatcgc ggcccccgcc gcccccggcc gcccgggccc acgggcgccg       60

tcccaaccgc acagtcccag                                                   80


<210>  5
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human herpesvirus 1 from BstEII site to start codon

<400>  5
ggttacctgg gactgtgcgg ttgggacggc gcccgtgggc ccgggcggcc gggggcggcg       60

ggggccgcga tggcggcggc ggcgggccat                                        90


<210>  6
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human herpesvirus 1 from BstEII cut site to BtgZI cut site

<400>  6
gttacctggg actgtgcggt tgggacggcg cccgtgggcc cgggcggccg ggggcggcgg       60

gggccgcgat ggcggcggcg g                                                 81


<210>  7
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Restriction Enzyme cleavage site for BtgZI


<220>
<221>  misc_feature
<222>  (7)..(16)
<223>  n is a, c, g, or t

<400>  7
gcgatgnnnn nnnnnn                                                       16


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Restriction ENzyne cleavage site for BtgZI


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  n is a, c, g, or t

<400>  8
nnnnnnnnnn nnnncatcgc                                                   20


<210>  9
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Restriction Enzyme cleavage site for PfIMI


<220>
<221>  misc_feature
<222>  (4)..(8)
<223>  n is a, c, g, or t

<400>  9
ccannnnntg g                                                            11


<210>  10
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse DNA complement sequence of the H3 miRNA

<400>  10
cccacgggcg ccgtcccaac cgcacagtcc caggtaacct ccacgcccaa ctcggaaccc       60

gcggtcagga gcgcgcccgc gg                                                82


<210>  11
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  H3 miRA sequence

<400>  11
cugggacugu gcgguuggga c                                                 21


<210>  12
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HSV-1 mjRNA miR-H3

<400>  12
gggcggccgc gggcgcgcuc cugaccgcgg guuccgaguu gggcgug                     47


<210>  13
<211>  44
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HSV-1 miRNA miR-H3

<400>  13
gagguuaccu gggacugugc gguugggacg gcgcccgugg gccc                        44


<210>  14
<211>  45
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HSV-1 miRNA miR-H4

<400>  14
gcgugccggg gugguagagu uugacaggca agcaugugcg ugcag                       45


<210>  15
<211>  43
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HSV-1 miRNA miR-H4

<400>  15
aggcgaguag ugcuugccug ucuaacucgc uagucucggc cgc                         43


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BtgZI restriction site

<400>  16
cccgccgccg ccgccatcgc                                                   20


<210>  17
<211>  125
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  attR1

<400>  17
acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta       60

aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca      120

tattg                                                                  125


<210>  18
<211>  125
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  attR2

<400>  18
catagtgact ggatatgttg tgttttacag cattatgtag tctgtttttt atgcaaaatc       60

taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa      120

gtggt                                                                  125


<210>  19
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  attL1

<400>  19
caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa       60

tgctttttta taatgccaac tttgtacaaa aaagcaggct                            100


<210>  20
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  attL2

<400>  20
acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca       60

acgaacaggt cactatcagt caaaataaaa tcattatttg                            100


<210>  21
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-H4-3p

<400>  21
actagcgagt tagacaggca ag                                                22


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-H4-5p in ICP34.5 5'UTR

<400>  22
tgcttgcctg tcaaactcta cc                                                22


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-H3-3p in ICP34.5

<400>  23
gtcccaaccg cacagtccca g                                                 21


<210>  24
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-H3-5p in ICP34.5

<400>  24
actcggaacc cgcggtcagg ag                                                22


<210>  25
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DR1 element

<400>  25
agcccgggcc ccccgcgg                                                     18


<210>  26
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PfIMI site

<400>  26
cgccacctgg tgg                                                          13


<210>  27
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-H4-3p

<400>  27
cttgcctgtc taactcgcta gt                                                22


<210>  28
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-H4-5p in ICP34.5 5'UTR

<400>  28
ggtagagttt gacaggcaag ca                                                22


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-H3-3p in ICP34.5

<400>  29
ctgggactgt gcggttggga c                                                 21


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-H3-5p in ICP34.5

<400>  30
ctcctgaccg cgggttccga gt                                                22


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement of DR1 element

<400>  31
gcccgcgggg ggcccgggct                                                   20


<210>  32
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer sequence

<400>  32
caggcacggc ccgatgaccg cctc                                              24


<210>  33
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement of DR1 element

<400>  33
ccgcgggggg cccgggct                                                     18


<210>  34
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer sequence

<400>  34
cgttttcggc gtctggcc                                                     18


<210>  35
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BtgZI site

<400>  35
gcgatggcgg cggcgg                                                       16


<210>  36
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PfIMI site

<400>  36
ccaccaggtg g                                                            11


