                         SEQUENCE LISTING

<110>  Resolution Bioscience, Inc.
       Raymond, Christopher K
       Lim, Lee P
       Armour, Christopher D
 
<120>  HIGH EFFICIENCY CONSTRUCTION OF DNA LIBRARIES

<130>  CLFK-003/01WO

<150>  US 62/254,110
<151>  2015-11-11

<160>  62    

<170>  PatentIn version 3.5

<210>  1
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide


<220>
<221>  misc_feature
<222>  (17)..(21)
<223>  n is A, C, T or G

<400>  1
gagggtctac cttcttnnnn ntgtattcga attctctggt cctgca                      46


<210>  2
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide


<220>
<221>  misc_feature
<222>  (17)..(21)
<223>  n is A, C, T or G

<400>  2
gctctagacg tcatcgnnnn ntgtattcga attctctggt cctgca                      46


<210>  3
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide


<220>
<221>  misc_feature
<222>  (17)..(21)
<223>  n is A, C, T or G

<400>  3
ggatactcgt agcggcnnnn ntgtattcga attctctggt cctgca                      46


<210>  4
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide


<220>
<221>  misc_feature
<222>  (17)..(21)
<223>  n is A, C, T or G

<400>  4
gtcacgagta gagaaannnn ntgtattcga attctctggt cctgca                      46


<210>  5
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide


<220>
<221>  misc_feature
<222>  (17)..(21)
<223>  n is A, C, T or G

<400>  5
gagggtctac cttagtnnnn ntgtattcga attctctggt cctgca                      46


<210>  6
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide


<220>
<221>  misc_feature
<222>  (17)..(21)
<223>  n is A, C, T or G

<400>  6
gctctagacg tcagagnnnn ntgtattcga attctctggt cctgca                      46


<210>  7
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide


<220>
<221>  misc_feature
<222>  (17)..(21)
<223>  n is A, C, T or G

<400>  7
ggatactcgt agcttcnnnn ntgtattcga attctctggt cctgca                      46


<210>  8
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide


<220>
<221>  misc_feature
<222>  (17)..(21)
<223>  n is A, C, T or G

<400>  8
gtcacgagta gagccannnn ntgtattcga attctctggt cctgca                      46


<210>  9
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide


<220>
<221>  misc_feature
<222>  (17)..(21)
<223>  n is A, C, T or G

<400>  9
gagggtctac cttgctnnnn ntgtattcga attctctggt cctgca                      46


<210>  10
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized partner strand oligonucleotide

<400>  10
aaggtagacc ct                                                           12


<210>  11
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized partner strand oligonucleotide

<400>  11
tgacgtctag ag                                                           12


<210>  12
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized partner strand oligonucleotide

<400>  12
gctacgagta tc                                                           12


<210>  13
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized partner strand oligonucleotide

<400>  13
ctctactcgt ga                                                           12


<210>  14
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized partner strand oligonucleotide

<400>  14
aaggtagacc ct                                                           12


<210>  15
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized partner strand oligonucleotide

<400>  15
tgacgtctag ag                                                           12


<210>  16
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized partner strand oligonucleotide

<400>  16
gctacgagta tc                                                           12


<210>  17
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized partner strand oligonucleotide

<400>  17
ctctactcgt ga                                                           12


<210>  18
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized partner strand oligonucleotide

<400>  18
aaggtagacc ct                                                           12


<210>  19
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  19
ctgagctagt                                                              10


<210>  20
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  20
gactcgatag                                                              10


<210>  21
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  21
tcagatcgtc                                                              10


<210>  22
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  22
agtctagcca                                                              10


<210>  23
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  23
ggattaccct                                                              10


<210>  24
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  24
cttacggatg                                                              10


<210>  25
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  25
accgattgac                                                              10


<210>  26
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  26
tagcgcatga                                                              10


<210>  27
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  27
atgtccagct                                                              10


<210>  28
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  28
cacaggttag                                                              10


<210>  29
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  29
tgacatgctc                                                              10


<210>  30
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  30
gctgtacaga                                                              10


<210>  31
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  31
tcaagtcggt                                                              10


<210>  32
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  32
gttcagactg                                                              10


<210>  33
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  33
caggtctaac                                                              10


<210>  34
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  34
agctcagtca                                                              10


<210>  35
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  35
gatccgtact                                                              10


<210>  36
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  36
acagtcgtag                                                              10


<210>  37
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  37
tggtaacctc                                                              10


<210>  38
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized ligation strand oligonucleotide

<400>  38
ctcagtagga                                                              10


<210>  39
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  39
tgcaggacca gagaattcga atacannnnn actagctcag                             40


<210>  40
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  40
tgcaggacca gagaattcga atacannnnn ctatcgagtc                             40


<210>  41
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  41
tgcaggacca gagaattcga atacannnnn gacgatctga                             40


<210>  42
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  42
tgcaggacca gagaattcga atacannnnn tggctagact                             40


<210>  43
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  43
tgcaggacca gagaattcga atacannnnn agggtaatcc                             40


<210>  44
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  44
tgcaggacca gagaattcga atacannnnn catccgtaag                             40


<210>  45
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  45
tgcaggacca gagaattcga atacannnnn gtcaatcggt                             40


<210>  46
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  46
tgcaggacca gagaattcga atacannnnn tcatgcgcta                             40


<210>  47
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  47
tgcaggacca gagaattcga atacannnnn actgctagca                             40


<210>  48
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  48
tgcaggacca gagaattcga atacannnnn cagcgatcat                             40


<210>  49
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  49
tgcaggacca gagaattcga atacannnnn gtcatcgatg                             40


<210>  50
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  50
tgcaggacca gagaattcga atacannnnn tgatagctgc                             40


<210>  51
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  51
tgcaggacca gagaattcga atacannnnn agctggacat                             40


<210>  52
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  52
tgcaggacca gagaattcga atacannnnn ctaacctgtg                             40


<210>  53
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  53
tgcaggacca gagaattcga atacannnnn gagcatgtca                             40


<210>  54
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  54
tgcaggacca gagaattcga atacannnnn tctgtacagc                             40


<210>  55
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  55
tgcaggacca gagaattcga atacannnnn accgacttga                             40


<210>  56
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  56
tgcaggacca gagaattcga atacannnnn cagtctgaac                             40


<210>  57
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  57
tgcaggacca gagaattcga atacannnnn gttagacctg                             40


<210>  58
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  58
tgcaggacca gagaattcga atacannnnn tgactgagct                             40


<210>  59
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  59
tgcaggacca gagaattcga atacannnnn agtacggatc                             40


<210>  60
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  60
tgcaggacca gagaattcga atacannnnn ctacgactgt                             40


<210>  61
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  61
tgcaggacca gagaattcga atacannnnn gaggttacca                             40


<210>  62
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized repair oligonucleotide


<220>
<221>  misc_feature
<222>  (26)..(30)
<223>  n is A, C, T or G, where the specific groups of bases from 
       positions 26 to 30 are listed in Table 3

<400>  62
tgcaggacca gagaattcga atacannnnn tcctactgag                             40


