                         SEQUENCE LISTING

<110>  ProteoThera, Inc.
 
<120>  METHODS OF PRODUCING AND PURIFYING MATRIX-BINDING FUSION PROTEINS
        BY ION-EXCHANGE CHROMATOGRAPHY

<130>  51053-006WO2

<150>  US 62/253,277
<151>  2015-11-10

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  1

Lys Lys Lys Arg Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro Cys 
1               5                   10                  15      


Leu Lys Lys Tyr Lys Gly 
            20          


<210>  2
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  2

Met Lys Arg Lys Lys Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro 
1               5                   10                  15      


Cys Leu Arg Lys Tyr Lys 
            20          


<210>  3
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  3

Lys Arg Lys Lys Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro Cys 
1               5                   10                  15      


Leu Arg Lys Tyr Lys 
            20      


<210>  4
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  4

Met Lys Arg Lys Lys Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro 
1               5                   10                  15      


Ser Leu Arg Lys Tyr Lys 
            20          


<210>  5
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  5

Met Lys Arg Lys Lys Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro 
1               5                   10                  15      


Arg Leu Arg Lys Tyr Lys 
            20          


<210>  6
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  6

Met Lys Arg Lys Lys Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro 
1               5                   10                  15      


Lys Leu Arg Lys Tyr Lys 
            20          


<210>  7
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  7

Lys Arg Lys Lys Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro Ser 
1               5                   10                  15      


Leu Arg Lys Tyr Lys 
            20      


<210>  8
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  8

Lys Arg Lys Lys Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro Arg 
1               5                   10                  15      


Leu Arg Lys Tyr Lys 
            20      


<210>  9
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  9

Lys Arg Lys Lys Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro Lys 
1               5                   10                  15      


Leu Arg Lys Tyr Lys 
            20      


<210>  10
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  10

Arg Ile Gln Asn Leu Leu Lys Ile Thr Asn Leu Arg Ile Lys Phe Val 
1               5                   10                  15      


Lys 
    


<210>  11
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  11

Arg Tyr Val Val Leu Pro Arg Pro Val Cys Phe Glu Lys Gly Thr Asn 
1               5                   10                  15      


Tyr Thr Val Arg 
            20  


<210>  12
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  12

Lys Gln Asn Cys Leu Ser Ser Arg Ala Ser Phe Arg Gly Cys Val Arg 
1               5                   10                  15      


Asn Leu Arg Leu Ser Arg 
            20          


<210>  13
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  13

Tyr Lys Lys Ile Ile Lys Lys Leu 
1               5               


<210>  14
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  14

Cys Lys Asn Gly Gly Phe Phe Leu Arg Ile His Pro Asp Gly Arg Val 
1               5                   10                  15      


Asp Gly Val Arg Glu Lys 
            20          


<210>  15
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  15

Tyr Thr Ser Trp Tyr Val Ala Leu Lys Arg Thr Gly Gln Tyr Lys Leu 
1               5                   10                  15      


Gly Ser Lys Thr Gly Pro Gly Gln Lys Ala Ile Leu Phe Leu Pro 
            20                  25                  30      


<210>  16
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  16

Ala Lys Leu Asn Cys Arg Leu Tyr Arg Lys Ala Asn Lys Ser Ser Lys 
1               5                   10                  15      


Leu Val Ser Ala Asn Arg Leu Phe Gly Asp Lys 
            20                  25          


<210>  17
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  17

Leu Arg Lys Leu Arg Lys Arg Leu Leu Arg Asp Ala Asp Asp Leu Gln 
1               5                   10                  15      


Lys Arg Leu Ala Val Tyr Gln 
            20              


<210>  18
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  18

Pro Leu Gln Glu Arg Ala Gln Ala Trp Gly Gln Glu Arg Leu Arg Ala 
1               5                   10                  15      


Arg Met Glu Glu Met Gly Ser Arg Thr Arg Asp Arg Leu Asp Glu Val 
            20                  25                  30          


Lys Glu Gln Val Ala Glu Arg Ala Lys Leu 
        35                  40          


<210>  19
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  19

Lys Gly Lys Met His Lys Thr Cys Tyr Phe 
1               5                   10  


<210>  20
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  20

Lys His Lys Gly Arg Asp Val Ile Leu Lys Lys Asp Val Arg 
1               5                   10                  


<210>  21
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  21

Lys Lys His Ala Glu Lys Asn Trp Phe Val Gly Leu Lys Lys Asn Gly 
1               5                   10                  15      


Ser Cys Lys Arg Gly Pro 
            20          


<210>  22
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  22

Lys Gly Gly Arg Gly Thr Pro Gly Lys Pro Gly Pro Arg Gly Gln Arg 
1               5                   10                  15      


Gly Pro Thr Gly Pro Arg Gly Glu Arg Gly Pro Arg Gly Ile Thr Gly 
            20                  25                  30          


Lys 
    


<210>  23
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  23

Gly Glu Phe Tyr Asp Leu Arg Leu Lys Gly Asp Lys 
1               5                   10          


<210>  24
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  24

His Arg His His Pro Arg Glu Met Lys Lys Arg Val Glu Asp Leu 
1               5                   10                  15  


<210>  25
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  25

Glu Lys Thr Leu Arg Lys Trp Leu Lys Met Phe Lys Lys Arg 
1               5                   10                  


<210>  26
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  26

Arg Arg Arg Pro Lys Gly Arg Gly Lys Arg Arg Arg Glu Lys Gln Arg 
1               5                   10                  15      


Pro Thr Asp Cys His Leu 
            20          


<210>  27
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain

<400>  27

Gln Pro Thr Arg Arg Pro Arg Pro Gly Thr Gly Pro Gly Arg Arg Pro 
1               5                   10                  15      


Arg Pro Arg Pro Arg Pro 
            20          


<210>  28
<211>  178
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain-IL-1RA fusion protein

<400>  28

Met Lys Arg Lys Lys Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro 
1               5                   10                  15      


Cys Leu Arg Lys Tyr Lys Gly Gly Gly Ser Arg Pro Ser Gly Arg Lys 
            20                  25                  30          


Ser Ser Lys Met Gln Ala Phe Arg Ile Trp Asp Val Asn Gln Lys Thr 
        35                  40                  45              


Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly Pro 
    50                  55                  60                  


Asn Val Asn Leu Glu Glu Lys Ile Asp Val Val Pro Ile Glu Pro His 
65                  70                  75                  80  


Ala Leu Phe Leu Gly Ile His Gly Gly Lys Met Cys Leu Ser Cys Val 
                85                  90                  95      


Lys Ser Gly Asp Glu Thr Arg Leu Gln Leu Glu Ala Val Asn Ile Thr 
            100                 105                 110         


Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys Arg Phe Ala Phe Ile Arg 
        115                 120                 125             


Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly 
    130                 135                 140                 


Trp Phe Leu Cys Thr Ala Met Glu Ala Asp Gln Pro Val Ser Leu Thr 
145                 150                 155                 160 


Asn Met Pro Asp Glu Gly Val Met Val Thr Lys Phe Tyr Phe Gln Glu 
                165                 170                 175     


Asp Glu 
        


<210>  29
<211>  178
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Matrix-binding domain-IL-1RA fusion protein

<400>  29

Met Lys Arg Lys Lys Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro 
1               5                   10                  15      


Arg Leu Arg Lys Tyr Lys Gly Gly Gly Ser Arg Pro Ser Gly Arg Lys 
            20                  25                  30          


Ser Ser Lys Met Gln Ala Phe Arg Ile Trp Asp Val Asn Gln Lys Thr 
        35                  40                  45              


Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly Pro 
    50                  55                  60                  


Asn Val Asn Leu Glu Glu Lys Ile Asp Val Val Pro Ile Glu Pro His 
65                  70                  75                  80  


Ala Leu Phe Leu Gly Ile His Gly Gly Lys Met Cys Leu Ser Cys Val 
                85                  90                  95      


Lys Ser Gly Asp Glu Thr Arg Leu Gln Leu Glu Ala Val Asn Ile Thr 
            100                 105                 110         


Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys Arg Phe Ala Phe Ile Arg 
        115                 120                 125             


Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly 
    130                 135                 140                 


Trp Phe Leu Cys Thr Ala Met Glu Ala Asp Gln Pro Val Ser Leu Thr 
145                 150                 155                 160 


Asn Met Pro Asp Glu Gly Val Met Val Thr Lys Phe Tyr Phe Gln Glu 
                165                 170                 175     


Asp Glu 
        


<210>  30
<211>  98
<212>  PRT
<213>  Homo sapiens - IGF-1

<400>  30

Gly Pro Glu Thr Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Gln Phe 
1               5                   10                  15      


Val Cys Gly Asp Arg Gly Phe Tyr Phe Asn Lys Pro Thr Gly Tyr Gly 
            20                  25                  30          


Ser Ser Ser Arg Arg Ala Pro Gln Thr Gly Ile Val Asp Glu Cys Cys 
        35                  40                  45              


Phe Arg Ser Cys Asp Leu Arg Arg Leu Glu Met Tyr Cys Ala Pro Leu 
    50                  55                  60                  


Lys Pro Ala Lys Ser Ala Arg Ser Val Arg Ala Gln Arg His Thr Asp 
65                  70                  75                  80  


Met Pro Lys Thr Gln Lys Glu Val His Leu Lys Asn Ala Ser Arg Gly 
                85                  90                  95      


Ser Ala 
        


