                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  FERMENTATION PROCESSES WITH REDUCED FOAMING

<130>  13241-WO-PCT

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  1275
<212>  DNA
<213>  Thermococcus litoralis

<400>  1
atggaattta acaaagtttt ttctctgctg ttggtctttg ttgtacttgg agctacagcg       60

gggatagtag gggcagtgtc tgccgagaaa gttcgggtga taataacaat agacaaggac      120

tttaacgaaa actccgtctt tgcacttgga ggaaacgttg ttgcaagagg aaaggtattt      180

ccaatcgtta tagcggagct ttctccacga gcagttgaaa ggctaaagaa tgctaagggt      240

gtcgtgagag tagagtacga tgcagaagtg caggtattaa agggcaaatc cccgggagca      300

ggcaagccaa agccttcaca accagctcaa acgattccat ggggaattga aaggattaaa      360

gccccggatg tatggagcat aactgacggt tcaagtagtg gagtaattga ggttgcaatc      420

ctagatactg gaattgatta tgaccatcca gatttagcgg caaatctcgc gtggggtgta      480

agcgtactta ggggcaaagt gtccacaaag cccaaagatt acaaagacca gaatggccat      540

gggactcatg ttgcgggaac tgtagcggca ctcaataatg acattggagt tgtaggagtc      600

gccccagctg tggagatcta tgctgttagg gttcttgatg caagcggtag aggatcctat      660

agcgacataa tccttggaat agagcaagca ctgcttggtc ccgatggagt tcttgacagt      720

gacggagatg gaataatagt gggtgatccg gatgatgatg cggccgaagt cataagcatg      780

agccttggag gtttaagcga tgttcaagcc ttccatgatg caataataga ggcatacaat      840

tacggagtag tcattgtggc ggcaagtggt aatgagggag cctcaagccc aagctatcca      900

gcagcttatc cggaggttat agccgttggg gcaactgacg ttaatgatca agtaccttgg      960

tggagcaaca ggggagtgga agtaagtgct cctggcgttg atgtactaag cacgtatccg     1020

gacgatagtt atgagacgct tagcggcact tcaatggcaa caccccatgt aagcggagtt     1080

gtggcgctaa tccaagcggc gtactacaac aaatatggaa gtgttcttcc ggttggaacg     1140

tttgatgata ataccatgag cactgttagg ggaattctac acatcacggc tgacgacctt     1200

ggaagctcgg gttgggatgc agactatggt tatggaatag ttagagcgga tttagctgtt     1260

caagctgtca actga                                                      1275


<210>  2
<211>  424
<212>  PRT
<213>  Thermococcus litoralis

<400>  2

Met Glu Phe Asn Lys Val Phe Ser Leu Leu Leu Val Phe Val Val Leu 
1               5                   10                  15      


Gly Ala Thr Ala Gly Ile Val Gly Ala Val Ser Ala Glu Lys Val Arg 
            20                  25                  30          


Val Ile Ile Thr Ile Asp Lys Asp Phe Asn Glu Asn Ser Val Phe Ala 
        35                  40                  45              


Leu Gly Gly Asn Val Val Ala Arg Gly Lys Val Phe Pro Ile Val Ile 
    50                  55                  60                  


Ala Glu Leu Ser Pro Arg Ala Val Glu Arg Leu Lys Asn Ala Lys Gly 
65                  70                  75                  80  


Val Val Arg Val Glu Tyr Asp Ala Glu Val Gln Val Leu Lys Gly Lys 
                85                  90                  95      


Ser Pro Gly Ala Gly Lys Pro Lys Pro Ser Gln Pro Ala Gln Thr Ile 
            100                 105                 110         


Pro Trp Gly Ile Glu Arg Ile Lys Ala Pro Asp Val Trp Ser Ile Thr 
        115                 120                 125             


Asp Gly Ser Ser Ser Gly Val Ile Glu Val Ala Ile Leu Asp Thr Gly 
    130                 135                 140                 


Ile Asp Tyr Asp His Pro Asp Leu Ala Ala Asn Leu Ala Trp Gly Val 
145                 150                 155                 160 


Ser Val Leu Arg Gly Lys Val Ser Thr Lys Pro Lys Asp Tyr Lys Asp 
                165                 170                 175     


Gln Asn Gly His Gly Thr His Val Ala Gly Thr Val Ala Ala Leu Asn 
            180                 185                 190         


Asn Asp Ile Gly Val Val Gly Val Ala Pro Ala Val Glu Ile Tyr Ala 
        195                 200                 205             


Val Arg Val Leu Asp Ala Ser Gly Arg Gly Ser Tyr Ser Asp Ile Ile 
    210                 215                 220                 


Leu Gly Ile Glu Gln Ala Leu Leu Gly Pro Asp Gly Val Leu Asp Ser 
225                 230                 235                 240 


Asp Gly Asp Gly Ile Ile Val Gly Asp Pro Asp Asp Asp Ala Ala Glu 
                245                 250                 255     


Val Ile Ser Met Ser Leu Gly Gly Leu Ser Asp Val Gln Ala Phe His 
            260                 265                 270         


Asp Ala Ile Ile Glu Ala Tyr Asn Tyr Gly Val Val Ile Val Ala Ala 
        275                 280                 285             


Ser Gly Asn Glu Gly Ala Ser Ser Pro Ser Tyr Pro Ala Ala Tyr Pro 
    290                 295                 300                 


Glu Val Ile Ala Val Gly Ala Thr Asp Val Asn Asp Gln Val Pro Trp 
305                 310                 315                 320 


Trp Ser Asn Arg Gly Val Glu Val Ser Ala Pro Gly Val Asp Val Leu 
                325                 330                 335     


Ser Thr Tyr Pro Asp Asp Ser Tyr Glu Thr Leu Ser Gly Thr Ser Met 
            340                 345                 350         


Ala Thr Pro His Val Ser Gly Val Val Ala Leu Ile Gln Ala Ala Tyr 
        355                 360                 365             


Tyr Asn Lys Tyr Gly Ser Val Leu Pro Val Gly Thr Phe Asp Asp Asn 
    370                 375                 380                 


Thr Met Ser Thr Val Arg Gly Ile Leu His Ile Thr Ala Asp Asp Leu 
385                 390                 395                 400 


Gly Ser Ser Gly Trp Asp Ala Asp Tyr Gly Tyr Gly Ile Val Arg Ala 
                405                 410                 415     


Asp Leu Ala Val Gln Ala Val Asn 
            420                 


<210>  3
<211>  1281
<212>  DNA
<213>  Artificial

<220>
<223>  Expression construct including Savinase signal peptid instead of 
       native signal

<400>  3
atgaaaaaac cgctggggaa aattgtcgca agcaccgcac tactcatttc tgttgctttt       60

agttcatcga tcgcatcggc tgtgtctgcc gagaaagttc gggtgataat aacaatagac      120

aaggacttta acgaaaactc cgtctttgca cttggaggaa acgttgttgc aagaggaaag      180

gtatttccaa tcgttatagc ggagctttct ccacgagcag ttgaaaggct aaagaatgct      240

aagggtgtcg tgagagtaga gtacgatgca gaagtgcagg tattaaaggg caaatccccg      300

ggagcaggca agccaaagcc ttcacaacca gctcaaacga ttccatgggg aattgaaagg      360

attaaagccc cggatgtatg gagcataact gacggttcaa gtagtggagt aattgaggtt      420

gcaatcctag atactggaat tgattatgac catccagatt tagcggcaaa tctcgcgtgg      480

ggtgtaagcg tacttagggg caaagtgtcc acaaagccca aagattacaa agaccagaat      540

ggccatggga ctcatgttgc gggaactgta gcggcactca ataatgacat tggagttgta      600

ggagtcgccc cagctgtgga gatctatgct gttagggttc ttgatgcaag cggtagagga      660

tcctatagcg acataatcct tggaatagag caagcactgc ttggtcccga tggagttctt      720

gacagtgacg gagatggaat aatagtgggt gatccggatg atgatgcggc cgaagtcata      780

agcatgagcc ttggaggttt aagcgatgtt caagccttcc atgatgcaat aatagaggca      840

tacaattacg gagtagtcat tgtggcggca agtggtaatg agggagcctc aagcccaagc      900

tatccagcag cttatccgga ggttatagcc gttggggcaa ctgacgttaa tgatcaagta      960

ccttggtgga gcaacagggg agtggaagta agtgctcctg gcgttgatgt actaagcacg     1020

tatccggacg atagttatga gacgcttagc ggcacttcaa tggcaacacc ccatgtaagc     1080

ggagttgtgg cgctaatcca agcggcgtac tacaacaaat atggaagtgt tcttccggtt     1140

ggaacgtttg atgataatac catgagcact gttaggggaa ttctacacat cacggctgac     1200

gaccttggaa gctcgggttg ggatgcagac tatggttatg gaatagttag agcggattta     1260

gctgttcaag ctgtcaactg a                                               1281


<210>  4
<211>  27
<212>  PRT
<213>  Bacillus clausii

<400>  4

Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile 
1               5                   10                  15      


Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala 
            20                  25          


<210>  5
<211>  515
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  5

Ala Ala Pro Phe Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr Leu 
1               5                   10                  15      


Pro Asp Asp Gly Thr Leu Trp Thr Lys Val Ala Asn Glu Ala Asn Asn 
            20                  25                  30          


Leu Ser Ser Leu Gly Ile Thr Ala Leu Trp Leu Pro Pro Ala Tyr Lys 
        35                  40                  45              


Gly Thr Ser Arg Ser Asp Val Gly Tyr Gly Val Tyr Asp Leu Tyr Asp 
    50                  55                  60                  


Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr 
65                  70                  75                  80  


Lys Ala Gln Tyr Leu Gln Ala Ile Gln Ala Ala His Ala Ala Gly Met 
                85                  90                  95      


Gln Val Tyr Ala Asp Val Val Phe Asp His Lys Gly Gly Ala Asp Gly 
            100                 105                 110         


Thr Glu Trp Val Asp Ala Val Glu Val Asn Pro Ser Asp Arg Asn Gln 
        115                 120                 125             


Glu Ile Ser Gly Thr Tyr Gln Ile Gln Ala Trp Thr Lys Phe Asp Phe 
    130                 135                 140                 


Pro Gly Arg Gly Asn Thr Tyr Ser Ser Phe Lys Trp Arg Trp Tyr His 
145                 150                 155                 160 


Phe Asp Gly Val Asp Trp Asp Glu Ser Arg Lys Leu Ser Arg Ile Tyr 
                165                 170                 175     


Lys Phe Arg Gly Ile Gly Lys Ala Trp Asp Trp Glu Val Asp Thr Glu 
            180                 185                 190         


Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Leu Asp Met Asp His 
        195                 200                 205             


Pro Glu Val Val Thr Glu Leu Lys Asn Trp Gly Lys Trp Tyr Val Asn 
    210                 215                 220                 


Thr Thr Asn Ile Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys 
225                 230                 235                 240 


Phe Ser Phe Phe Pro Asp Trp Leu Ser Tyr Val Arg Ser Gln Thr Gly 
                245                 250                 255     


Lys Pro Leu Phe Thr Val Gly Glu Tyr Trp Ser Tyr Asp Ile Asn Lys 
            260                 265                 270         


Leu His Asn Tyr Ile Thr Lys Thr Asn Gly Thr Met Ser Leu Phe Asp 
        275                 280                 285             


Ala Pro Leu His Asn Lys Phe Tyr Thr Ala Ser Lys Ser Gly Gly Ala 
    290                 295                 300                 


Phe Asp Met Arg Thr Leu Met Thr Asn Thr Leu Met Lys Asp Gln Pro 
305                 310                 315                 320 


Thr Leu Ala Val Thr Phe Val Asp Asn His Asp Thr Glu Pro Gly Gln 
                325                 330                 335     


Ala Leu Gln Ser Trp Val Asp Pro Trp Phe Lys Pro Leu Ala Tyr Ala 
            340                 345                 350         


Phe Ile Leu Thr Arg Gln Glu Gly Tyr Pro Cys Val Phe Tyr Gly Asp 
        355                 360                 365             


Tyr Tyr Gly Ile Pro Gln Tyr Asn Ile Pro Ser Leu Lys Ser Lys Ile 
    370                 375                 380                 


Asp Pro Leu Leu Ile Ala Arg Arg Asp Tyr Ala Tyr Gly Thr Gln His 
385                 390                 395                 400 


Asp Tyr Leu Asp His Ser Asp Ile Ile Gly Trp Thr Arg Glu Gly Val 
                405                 410                 415     


Thr Glu Lys Pro Gly Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly Pro 
            420                 425                 430         


Gly Gly Ser Lys Trp Met Tyr Val Gly Lys Gln His Ala Gly Lys Val 
        435                 440                 445             


Phe Tyr Asp Leu Thr Gly Asn Arg Ser Asp Thr Val Thr Ile Asn Ser 
    450                 455                 460                 


Asp Gly Trp Gly Glu Phe Lys Val Asn Gly Gly Ser Val Ser Val Trp 
465                 470                 475                 480 


Val Pro Arg Lys Thr Thr Val Ser Thr Ile Ala Arg Pro Ile Thr Thr 
                485                 490                 495     


Arg Pro Trp Thr Gly Glu Phe Val Arg Trp Thr Glu Pro Arg Leu Val 
            500                 505                 510         


Ala Trp Pro 
        515 


<210>  6
<211>  583
<212>  PRT
<213>  Artificial

<220>
<223>  Hybrid alpha-amylase

<400>  6

Ala Thr Ser Asp Asp Trp Lys Gly Lys Ala Ile Tyr Gln Leu Leu Thr 
1               5                   10                  15      


Asp Arg Phe Gly Arg Ala Asp Asp Ser Thr Ser Asn Cys Ser Asn Leu 
            20                  25                  30          


Ser Asn Tyr Cys Gly Gly Thr Tyr Glu Gly Ile Thr Lys His Leu Asp 
        35                  40                  45              


Tyr Ile Ser Gly Met Gly Phe Asp Ala Ile Trp Ile Ser Pro Ile Pro 
    50                  55                  60                  


Lys Asn Ser Asp Gly Gly Tyr His Gly Tyr Trp Ala Thr Asp Phe Tyr 
65                  70                  75                  80  


Gln Leu Asn Ser Asn Phe Gly Asp Glu Ser Gln Leu Lys Ala Leu Ile 
                85                  90                  95      


Gln Ala Ala His Glu Arg Asp Met Tyr Val Met Leu Asp Val Val Ala 
            100                 105                 110         


Asn His Ala Gly Pro Thr Ser Asn Gly Tyr Ser Gly Tyr Thr Phe Gly 
        115                 120                 125             


Asp Ala Ser Leu Tyr His Pro Lys Cys Thr Ile Asp Tyr Asn Asp Gln 
    130                 135                 140                 


Thr Ser Ile Glu Gln Cys Trp Val Ala Asp Glu Leu Pro Asp Ile Asp 
145                 150                 155                 160 


Thr Glu Asn Ser Asp Asn Val Ala Ile Leu Asn Asp Ile Val Ser Gly 
                165                 170                 175     


Trp Val Gly Asn Tyr Ser Phe Asp Gly Ile Arg Ile Asp Thr Val Lys 
            180                 185                 190         


His Ile Arg Lys Asp Phe Trp Thr Gly Tyr Ala Glu Ala Ala Gly Val 
        195                 200                 205             


Phe Ala Thr Gly Glu Val Phe Asn Gly Asp Pro Ala Tyr Val Gly Pro 
    210                 215                 220                 


Tyr Gln Lys Tyr Leu Pro Ser Leu Ile Asn Tyr Pro Met Tyr Tyr Ala 
225                 230                 235                 240 


Leu Asn Asp Val Phe Val Ser Lys Ser Lys Gly Phe Ser Arg Ile Ser 
                245                 250                 255     


Glu Met Leu Gly Ser Asn Arg Asn Ala Phe Glu Asp Thr Ser Val Leu 
            260                 265                 270         


Thr Thr Phe Val Asp Asn His Asp Asn Pro Arg Phe Leu Asn Ser Gln 
        275                 280                 285             


Ser Asp Lys Ala Leu Phe Lys Asn Ala Leu Thr Tyr Val Leu Leu Gly 
    290                 295                 300                 


Glu Gly Ile Pro Ile Val Tyr Tyr Gly Ser Glu Gln Gly Phe Ser Gly 
305                 310                 315                 320 


Gly Ala Asp Pro Ala Asn Arg Glu Val Leu Trp Thr Thr Asn Tyr Asp 
                325                 330                 335     


Thr Ser Ser Asp Leu Tyr Gln Phe Ile Lys Thr Val Asn Ser Val Arg 
            340                 345                 350         


Met Lys Ser Asn Lys Ala Val Tyr Met Asp Ile Tyr Val Gly Asp Asn 
        355                 360                 365             


Ala Tyr Ala Phe Lys His Gly Asp Ala Leu Val Val Leu Asn Asn Tyr 
    370                 375                 380                 


Gly Ser Gly Ser Thr Asn Gln Val Ser Phe Ser Val Ser Gly Lys Phe 
385                 390                 395                 400 


Asp Ser Gly Ala Ser Leu Met Asp Ile Val Ser Asn Ile Thr Thr Thr 
                405                 410                 415     


Val Ser Ser Asp Gly Thr Val Thr Phe Asn Leu Lys Asp Gly Leu Pro 
            420                 425                 430         


Ala Ile Phe Thr Ser Ala Thr Gly Gly Thr Thr Thr Thr Ala Thr Pro 
        435                 440                 445             


Thr Gly Ser Gly Ser Val Thr Ser Thr Ser Lys Thr Thr Ala Thr Ala 
    450                 455                 460                 


Ser Lys Thr Ser Thr Ser Thr Ser Ser Thr Ser Cys Thr Thr Pro Thr 
465                 470                 475                 480 


Ala Val Ala Val Thr Phe Asp Leu Thr Ala Thr Thr Thr Tyr Gly Glu 
                485                 490                 495     


Asn Ile Tyr Leu Val Gly Ser Ile Ser Gln Leu Gly Asp Trp Glu Thr 
            500                 505                 510         


Ser Asp Gly Ile Ala Leu Ser Ala Asp Lys Tyr Thr Ser Ser Asp Pro 
        515                 520                 525             


Leu Trp Tyr Val Thr Val Thr Leu Pro Ala Gly Glu Ser Phe Glu Tyr 
    530                 535                 540                 


Lys Phe Ile Arg Ile Glu Ser Asp Asp Ser Val Glu Trp Glu Ser Asp 
545                 550                 555                 560 


Pro Asn Arg Glu Tyr Thr Val Pro Gln Ala Cys Gly Thr Ser Thr Ala 
                565                 570                 575     


Thr Val Thr Asp Thr Trp Arg 
            580             


<210>  7
<211>  412
<212>  PRT
<213>  Pyrococcus furiosus

<400>  7

Ala Glu Leu Glu Gly Leu Asp Glu Ser Ala Ala Gln Val Met Ala Thr 
1               5                   10                  15      


Tyr Val Trp Asn Leu Gly Tyr Asp Gly Ser Gly Ile Thr Ile Gly Ile 
            20                  25                  30          


Ile Asp Thr Gly Ile Asp Ala Ser His Pro Asp Leu Gln Gly Lys Val 
        35                  40                  45              


Ile Gly Trp Val Asp Phe Val Asn Gly Arg Ser Tyr Pro Tyr Asp Asp 
    50                  55                  60                  


His Gly His Gly Thr His Val Ala Ser Ile Ala Ala Gly Thr Gly Ala 
65                  70                  75                  80  


Ala Ser Asn Gly Lys Tyr Lys Gly Met Ala Pro Gly Ala Lys Leu Ala 
                85                  90                  95      


Gly Ile Lys Val Leu Gly Ala Asp Gly Ser Gly Ser Ile Ser Thr Ile 
            100                 105                 110         


Ile Lys Gly Val Glu Trp Ala Val Asp Asn Lys Asp Lys Tyr Gly Ile 
        115                 120                 125             


Lys Val Ile Asn Leu Ser Leu Gly Ser Ser Gln Ser Ser Asp Gly Thr 
    130                 135                 140                 


Asp Ala Leu Ser Gln Ala Val Asn Ala Ala Trp Asp Ala Gly Leu Val 
145                 150                 155                 160 


Val Val Val Ala Ala Gly Asn Ser Gly Pro Asn Lys Tyr Thr Ile Gly 
                165                 170                 175     


Ser Pro Ala Ala Ala Ser Lys Val Ile Thr Val Gly Ala Val Asp Lys 
            180                 185                 190         


Tyr Asp Val Ile Thr Ser Phe Ser Ser Arg Gly Pro Thr Ala Asp Gly 
        195                 200                 205             


Arg Leu Lys Pro Glu Val Val Ala Pro Gly Asn Trp Ile Ile Ala Ala 
    210                 215                 220                 


Arg Ala Ser Gly Thr Ser Met Gly Gln Pro Ile Asn Asp Tyr Tyr Thr 
225                 230                 235                 240 


Ala Ala Pro Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ile Ala 
                245                 250                 255     


Ala Leu Leu Leu Gln Ala His Pro Ser Trp Thr Pro Asp Lys Val Lys 
            260                 265                 270         


Thr Ala Leu Ile Glu Thr Ala Asp Ile Val Lys Pro Asp Glu Ile Ala 
        275                 280                 285             


Asp Ile Ala Tyr Gly Ala Gly Arg Val Asn Ala Tyr Lys Ala Ile Asn 
    290                 295                 300                 


Tyr Asp Asn Tyr Ala Lys Leu Val Phe Thr Gly Tyr Val Ala Asn Lys 
305                 310                 315                 320 


Gly Ser Gln Thr His Gln Phe Val Ile Ser Gly Ala Ser Phe Val Thr 
                325                 330                 335     


Ala Thr Leu Tyr Trp Asp Asn Ala Asn Ser Asp Leu Asp Leu Tyr Leu 
            340                 345                 350         


Tyr Asp Pro Asn Gly Asn Gln Val Asp Tyr Ser Tyr Thr Ala Tyr Tyr 
        355                 360                 365             


Asp Phe Glu Lys Val Gly Tyr Tyr Asn Pro Thr Asp Gly Thr Trp Thr 
    370                 375                 380                 


Ile Lys Val Val Ser Tyr Ser Gly Ser Ala Asn Tyr Gln Val Asp Val 
385                 390                 395                 400 


Val Ser Asp Gly Ser Leu Ser Gln Pro Gly Ser Ser 
                405                 410         


<210>  8
<211>  1278
<212>  DNA
<213>  Thermococcus sp

<400>  8
atggaattta acaaagtttt ttctctgctg ttggtctttg ttgtacttgg agctacagca       60

gggatagtag gggcagcgcc tgctgagaaa gctcgagtga taataacaat agacaaggac      120

tttaacgaaa actccgtctt tgcacttgga ggcaacgttg ttgcaagagg aaaggtattt      180

ccaattgtta tagcggagct tcctccacga gcaattgaga gattaaagaa tgctaagggt      240

gttgttagag tagaatacga tgcggaggcc catatattaa aaggcaaacc accgggaaca      300

ggcaagccaa agccttcaca accagctcaa acgattccat ggggaattga aaggattaaa      360

gccccggatg catggagcat aactgatggt tcaagtggtg gagtaattga ggttgcaatc      420

ctcgatacgg gaattgatta tgaccatcca gatttagcgg caaatctcgc gtggggtgta      480

agcgtactta gaggcaaagt gtctacaaat cccaaagatt acaaagacca gaatggccat      540

gggactcatg ttgcgggaac tgtagcggca ctcaataatg acattggagt agtgggagtc      600

gcttcagctg tggagattta tgctgttagg gttcttgatg caagtggtag aggatcttat      660

agcgacataa tccttggaat agagcaggca ttgcttggcc ctgatggagt gcttgactcc      720

gataatgatg gtgtaatagt gggagatccg gacgatgatg cagctgaagt cataagcatg      780

agccttggag gttcaagcga tgttcaagcc ttccatgatg caataataga ggcatacaat      840

tacggagttg tcatcgtagc ggcaagtggt aatgatgggg catcaagtcc aagttaccct      900

gcagcttatc cagaggttat agccgttggt gcaacagata gcgatgacca agtaccttgg      960

tggagcaaca ggggagtaga agttagtgct cctggcgttg atatactaag cacgtatccg     1020

gacgatacct atgaaacact tagcggcact tcaatggcaa cacctcacgt tagtggagta     1080

gtggcattaa tccaggcggc gtactacaac aaatatgggt atgtccttcc agttggaaca     1140

tttggcgatc ttaccacgag tactgttagg gggattctac acgtaacagc tgatgacctt     1200

ggaagctcgg gttgggatgc agactatggc tatggaatag ttagggcaga tttggctgtt     1260

caagctgcta tcagttga                                                   1278


<210>  9
<211>  425
<212>  PRT
<213>  Thermococcus sp

<400>  9

Met Glu Phe Asn Lys Val Phe Ser Leu Leu Leu Val Phe Val Val Leu 
1               5                   10                  15      


Gly Ala Thr Ala Gly Ile Val Gly Ala Ala Pro Ala Glu Lys Ala Arg 
            20                  25                  30          


Val Ile Ile Thr Ile Asp Lys Asp Phe Asn Glu Asn Ser Val Phe Ala 
        35                  40                  45              


Leu Gly Gly Asn Val Val Ala Arg Gly Lys Val Phe Pro Ile Val Ile 
    50                  55                  60                  


Ala Glu Leu Pro Pro Arg Ala Ile Glu Arg Leu Lys Asn Ala Lys Gly 
65                  70                  75                  80  


Val Val Arg Val Glu Tyr Asp Ala Glu Ala His Ile Leu Lys Gly Lys 
                85                  90                  95      


Pro Pro Gly Thr Gly Lys Pro Lys Pro Ser Gln Pro Ala Gln Thr Ile 
            100                 105                 110         


Pro Trp Gly Ile Glu Arg Ile Lys Ala Pro Asp Ala Trp Ser Ile Thr 
        115                 120                 125             


Asp Gly Ser Ser Gly Gly Val Ile Glu Val Ala Ile Leu Asp Thr Gly 
    130                 135                 140                 


Ile Asp Tyr Asp His Pro Asp Leu Ala Ala Asn Leu Ala Trp Gly Val 
145                 150                 155                 160 


Ser Val Leu Arg Gly Lys Val Ser Thr Asn Pro Lys Asp Tyr Lys Asp 
                165                 170                 175     


Gln Asn Gly His Gly Thr His Val Ala Gly Thr Val Ala Ala Leu Asn 
            180                 185                 190         


Asn Asp Ile Gly Val Val Gly Val Ala Ser Ala Val Glu Ile Tyr Ala 
        195                 200                 205             


Val Arg Val Leu Asp Ala Ser Gly Arg Gly Ser Tyr Ser Asp Ile Ile 
    210                 215                 220                 


Leu Gly Ile Glu Gln Ala Leu Leu Gly Pro Asp Gly Val Leu Asp Ser 
225                 230                 235                 240 


Asp Asn Asp Gly Val Ile Val Gly Asp Pro Asp Asp Asp Ala Ala Glu 
                245                 250                 255     


Val Ile Ser Met Ser Leu Gly Gly Ser Ser Asp Val Gln Ala Phe His 
            260                 265                 270         


Asp Ala Ile Ile Glu Ala Tyr Asn Tyr Gly Val Val Ile Val Ala Ala 
        275                 280                 285             


Ser Gly Asn Asp Gly Ala Ser Ser Pro Ser Tyr Pro Ala Ala Tyr Pro 
    290                 295                 300                 


Glu Val Ile Ala Val Gly Ala Thr Asp Ser Asp Asp Gln Val Pro Trp 
305                 310                 315                 320 


Trp Ser Asn Arg Gly Val Glu Val Ser Ala Pro Gly Val Asp Ile Leu 
                325                 330                 335     


Ser Thr Tyr Pro Asp Asp Thr Tyr Glu Thr Leu Ser Gly Thr Ser Met 
            340                 345                 350         


Ala Thr Pro His Val Ser Gly Val Val Ala Leu Ile Gln Ala Ala Tyr 
        355                 360                 365             


Tyr Asn Lys Tyr Gly Tyr Val Leu Pro Val Gly Thr Phe Gly Asp Leu 
    370                 375                 380                 


Thr Thr Ser Thr Val Arg Gly Ile Leu His Val Thr Ala Asp Asp Leu 
385                 390                 395                 400 


Gly Ser Ser Gly Trp Asp Ala Asp Tyr Gly Tyr Gly Ile Val Arg Ala 
                405                 410                 415     


Asp Leu Ala Val Gln Ala Ala Ile Ser 
            420                 425 


<210>  10
<211>  1284
<212>  DNA
<213>  Artificial

<220>
<223>  Expression construct including Savinase signal peptid instead of 
       native signal

<400>  10
atgaagaaac cgttggggaa aattgtcgca agcaccgcac tactcatttc tgttgctttt       60

agttcatcga tagcatcagc agcaccagca gagaaggcac gtgttatcat cactattgat      120

aaggacttta acgagaattc agttttcgct ttaggtggta atgtagttgc tcgcggaaaa      180

gttttcccta ttgttatcgc ggaacttcct cctcgtgcaa tcgaacgttt gaaaaacgct      240

aaaggcgtag ttcgtgttga atacgatgcg gaagctcaca tccttaaagg caaacctccg      300

ggtactggta agccaaaacc gtctcaaccg gctcaaacta tcccgtgggg tatcgaacgt      360

atcaaagcac cggacgcatg gtctattaca gacggctctt ctggtggtgt aattgaagta      420

gcgatcttag atacaggaat cgactacgat catcctgatc ttgcagcgaa cttggcatgg      480

ggtgtatctg ttcttcgtgg taaagtatct actaacccta aagactacaa ggaccaaaac      540

ggacacggta cgcatgttgc aggaactgta gcagcgttga acaacgacat tggagttgtt      600

ggcgtagcgt ctgctgtaga gatctatgct gttcgtgttc ttgatgcgtc tggtcgtgga      660

agctattctg acatcattct tggaatcgaa caagcattac ttggtcctga cggagttttg      720

gattcagata acgatggtgt tatcgtaggt gaccctgatg acgacgctgc tgaagttatc      780

tcaatgagcc ttggtggctc ttcagacgtt caagccttcc atgacgcaat catcgaagct      840

tacaactatg gagttgttat tgttgcggca tctggaaacg acggtgcgtc aagcccttct      900

tacccagctg cttaccctga ggtaattgct gttggagcga cagattcaga tgaccaggta      960

ccttggtggt caaatcgcgg tgttgaagtt tctgctcctg gagttgatat ccttagcaca     1020

taccctgatg acacttacga aacactttca ggcacttcta tggctacacc tcatgtttct     1080

ggcgtagtag ctcttatcca agctgcgtat tacaacaaat acggttatgt tcttccagtt     1140

ggcacatttg gagatcttac gacgagcacg gttcgcggta ttcttcatgt tacagcggac     1200

gacttaggct cttctggctg ggatgctgat tatggttacg gtattgtacg tgctgactta     1260

gcagttcagg cagcaatcag ctaa                                            1284


<210>  11
<211>  366
<212>  PRT
<213>  Meripilus giganteus

<400>  11

Ala Ile Pro Ala Ser Cys Ala Ser Thr Ile Thr Pro Ala Cys Leu Gln 
1               5                   10                  15      


Ala Ile Tyr Gly Ile Pro Thr Thr Lys Ala Thr Gln Ser Ser Asn Lys 
            20                  25                  30          


Leu Ala Val Ser Gly Phe Ile Asp Gln Phe Ala Asn Lys Ala Asp Leu 
        35                  40                  45              


Lys Ser Phe Leu Ala Gln Phe Arg Lys Asp Ile Ser Ser Ser Thr Thr 
    50                  55                  60                  


Phe Ser Leu Gln Thr Leu Asp Gly Gly Glu Asn Asp Gln Ser Pro Ser 
65                  70                  75                  80  


Glu Ala Gly Ile Glu Ala Asn Leu Asp Ile Gln Tyr Thr Val Gly Leu 
                85                  90                  95      


Ala Thr Gly Val Pro Thr Thr Phe Ile Ser Val Gly Asp Asp Phe Gln 
            100                 105                 110         


Asp Gly Asn Leu Glu Gly Phe Leu Asp Ile Ile Asn Phe Leu Leu Gly 
        115                 120                 125             


Glu Ser Asn Pro Pro Gln Val Leu Thr Thr Ser Tyr Gly Gln Asn Glu 
    130                 135                 140                 


Asn Thr Ile Ser Ala Lys Leu Ala Asn Gln Leu Cys Asn Ala Tyr Ala 
145                 150                 155                 160 


Gln Leu Gly Ala Arg Gly Thr Ser Ile Leu Phe Ala Ser Gly Asp Gly 
                165                 170                 175     


Gly Val Ser Gly Ser Gln Ser Ala His Cys Ser Asn Phe Val Pro Thr 
            180                 185                 190         


Phe Pro Ser Gly Cys Pro Phe Met Thr Ser Val Gly Ala Thr Gln Gly 
        195                 200                 205             


Val Ser Pro Glu Thr Ala Ala Ala Phe Ser Ser Gly Gly Phe Ser Asn 
    210                 215                 220                 


Val Phe Gly Ile Pro Ser Tyr Gln Ala Ser Ala Val Ser Gly Tyr Leu 
225                 230                 235                 240 


Ser Ala Leu Gly Ser Thr Asn Ser Gly Lys Phe Asn Arg Ser Gly Arg 
                245                 250                 255     


Gly Phe Pro Asp Val Ser Thr Gln Gly Val Asp Phe Gln Ile Val Ser 
            260                 265                 270         


Gly Gly Gln Thr Ile Gly Val Asp Gly Thr Ser Cys Ala Ser Pro Thr 
        275                 280                 285             


Phe Ala Ser Val Ile Ser Leu Val Asn Asp Arg Leu Ile Ala Ala Gly 
    290                 295                 300                 


Lys Ser Pro Leu Gly Phe Leu Asn Pro Phe Leu Tyr Ser Ser Ala Gly 
305                 310                 315                 320 


Lys Ala Ala Leu Asn Asp Val Thr Ser Gly Ser Asn Pro Gly Cys Ser 
                325                 330                 335     


Thr Asn Gly Phe Pro Ala Lys Ala Gly Trp Asp Pro Val Thr Gly Leu 
            340                 345                 350         


Gly Thr Pro Asn Phe Ala Lys Leu Leu Thr Ala Val Gly Leu 
        355                 360                 365     


