                         SEQUENCE LISTING

<110>  Danisco US, Inc.
 
<120>  PAENIBACILLUS AND BACILLUS SPP. MANNANASES

<130>  NB40831-WO-PCT

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  894
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic

<400>  1
atggcgacag gcttttatgt ttcaggcaac aaactgtatg atagcacagg caaaccgttt       60

gttatgagag gcgttaatca tggccatagc tggtttaaaa acgatctgaa tacagcgatt      120

ccggctattg caaaaacagg cgcaaataca gttagaattg ttctgtcaaa tggcagcctg      180

tatacgaaag atgatctgaa tgcagtcaaa aacatcatca atgtcgtcaa ccagaacaaa      240

atgattgcag ttctggaagt tcatgatgca acgggcaaag atgattacaa ttcactggat      300

gcagcagtca actattggat ctcaattaaa gaagcgctga tcggcaaaga agatcgcgtt      360

attgttaata ttgcgaacga atggtatggc acatggaatg gctcagcatg ggcagatggc      420

tacaaaaaag caattccgaa actgagaaat gcaggcatca aaaacacact gattgttgat      480

gcggcaggct ggggacaatt tccgcaatca attgttgatt atggccaaag cgtttttgca      540

gcagatagcc agaaaaatac agtctttagc atccatatgt acgaatacgc tggaaaagat      600

gcagcaacag ttaaagcgaa tatggaaaac gtcctgaata aaggcctggc actgattatt      660

ggcgaatttg gcggatatca tacaaatggc gacgttgatg aatatgcgat tatgagatat      720

ggccaagaaa aaggcgttgg ctggcttgca tggtcatggt atggaaattc atcaggcctt      780

aactatctgg atatggcaac aggaccgaat ggatcactga catcatttgg caatacagtc      840

gtcaatgata cgtatggaat caaaaatacg agccagaaag ctggcatctt ttaa            894


<210>  2
<211>  297
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  2

Met Ala Thr Gly Phe Tyr Val Ser Gly Asn Lys Leu Tyr Asp Ser Thr 
1               5                   10                  15      


Gly Lys Pro Phe Val Met Arg Gly Val Asn His Gly His Ser Trp Phe 
            20                  25                  30          


Lys Asn Asp Leu Asn Thr Ala Ile Pro Ala Ile Ala Lys Thr Gly Ala 
        35                  40                  45              


Asn Thr Val Arg Ile Val Leu Ser Asn Gly Ser Leu Tyr Thr Lys Asp 
    50                  55                  60                  


Asp Leu Asn Ala Val Lys Asn Ile Ile Asn Val Val Asn Gln Asn Lys 
65                  70                  75                  80  


Met Ile Ala Val Leu Glu Val His Asp Ala Thr Gly Lys Asp Asp Tyr 
                85                  90                  95      


Asn Ser Leu Asp Ala Ala Val Asn Tyr Trp Ile Ser Ile Lys Glu Ala 
            100                 105                 110         


Leu Ile Gly Lys Glu Asp Arg Val Ile Val Asn Ile Ala Asn Glu Trp 
        115                 120                 125             


Tyr Gly Thr Trp Asn Gly Ser Ala Trp Ala Asp Gly Tyr Lys Lys Ala 
    130                 135                 140                 


Ile Pro Lys Leu Arg Asn Ala Gly Ile Lys Asn Thr Leu Ile Val Asp 
145                 150                 155                 160 


Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile Val Asp Tyr Gly Gln 
                165                 170                 175     


Ser Val Phe Ala Ala Asp Ser Gln Lys Asn Thr Val Phe Ser Ile His 
            180                 185                 190         


Met Tyr Glu Tyr Ala Gly Lys Asp Ala Ala Thr Val Lys Ala Asn Met 
        195                 200                 205             


Glu Asn Val Leu Asn Lys Gly Leu Ala Leu Ile Ile Gly Glu Phe Gly 
    210                 215                 220                 


Gly Tyr His Thr Asn Gly Asp Val Asp Glu Tyr Ala Ile Met Arg Tyr 
225                 230                 235                 240 


Gly Gln Glu Lys Gly Val Gly Trp Leu Ala Trp Ser Trp Tyr Gly Asn 
                245                 250                 255     


Ser Ser Gly Leu Asn Tyr Leu Asp Met Ala Thr Gly Pro Asn Gly Ser 
            260                 265                 270         


Leu Thr Ser Phe Gly Asn Thr Val Val Asn Asp Thr Tyr Gly Ile Lys 
        275                 280                 285             


Asn Thr Ser Gln Lys Ala Gly Ile Phe 
    290                 295         


<210>  3
<211>  608
<212>  DNA
<213>  Bacillus subtilis

<400>  3
gaattctcca ttttcttctg ctatcaaaat aacagactcg tgattttcca aacgagcttt       60

caaaaaagcc tctgcccctt gcaaatcgga tgcctgtcta taaaattccc gatattggtt      120

aaacagcggc gcaatggcgg ccgcatctga tgtctttgct tggcgaatgt tcatcttatt      180

tcttcctccc tctcaataat tttttcattc tatccctttt ctgtaaagtt tatttttcag      240

aatactttta tcatcatgct ttgaaaaaat atcacgataa tatccattgt tctcacggaa      300

gcacacgcag gtcatttgaa cgaatttttt cgacaggaat ttgccgggac tcaggagcat      360

ttaacctaaa aaagcatgac atttcagcat aatgaacatt tactcatgtc tattttcgtt      420

cttttctgta tgaaaatagt tatttcgagt ctctacggaa atagcgagag atgatatacc      480

taaatagaga taaaatcatc tcaaaaaaat gggtctacta aaatattatt ccatctatta      540

caataaattc acagaatagt cttttaagta agtctactct gaattttttt aaaaggagag      600

ggtaaaga                                                               608


<210>  4
<211>  87
<212>  DNA
<213>  Bacillus subtilis

<400>  4
gtgagaagca aaaaattgtg gatcagcttg ttgtttgcgt taacgttaat ctttacgatg       60

gcgttcagca acatgtctgc tagcgca                                           87


<210>  5
<211>  93
<212>  DNA
<213>  artificial sequence

<220>
<223>  terminator

<400>  5
ggttaccttg aatgtatata aacattctca aagggatttc taataaaaaa cgctcggttg       60

ccgccgggcg ttttttatgc atcgatggaa ttc                                    93


<210>  6
<211>  297
<212>  PRT
<213>  artificial sequence

<220>
<223>  variant

<400>  6

Met Ala Thr Gly Phe Tyr Val Ser Gly Asn Lys Leu Tyr Asp Ser Thr 
1               5                   10                  15      


Gly Lys Glu Phe Val Met Arg Gly Val Asn His Gly His Ser Trp Phe 
            20                  25                  30          


Lys Asn Asp Leu Asn Glu Ala Ile Pro Ala Ile Ala Lys Thr Gly Ala 
        35                  40                  45              


Asn Thr Val Arg Ile Val Leu Ser Asn Gly Ser Leu Tyr Thr Lys Asp 
    50                  55                  60                  


Asp Leu Asp Ala Val Lys Asn Ile Ile Asn Val Val Asn Gln Asn Lys 
65                  70                  75                  80  


Met Ile Ala Val Leu Glu Val His Asp Ala Thr Gly Lys Asp Asp Tyr 
                85                  90                  95      


Asp Ser Leu Asp Ala Ala Val Asn Tyr Trp Ile Ser Ile Lys Glu Ala 
            100                 105                 110         


Leu Ile Gly Lys Glu Asp Arg Val Ile Val Asn Ile Ala Asn Glu Trp 
        115                 120                 125             


Met Gly Thr Trp Asn Gly Ser Ala Trp Ala Asp Gly Tyr Lys Lys Ala 
    130                 135                 140                 


Ile Pro Lys Leu Arg Asn Ala Gly Ile Lys Asn Thr Leu Ile Val Asp 
145                 150                 155                 160 


Ala Ala Gly Trp Gly Gln Phe Ser Gln Ser Ile Val Asp Tyr Gly Gln 
                165                 170                 175     


Ser Val Phe Ala Ala Asp Ser Leu Lys Asn Thr Val Phe Ser Ile His 
            180                 185                 190         


Met Tyr Glu Tyr Ala Gly Lys Asp Ala Ala Thr Val Lys Ala Asn Met 
        195                 200                 205             


Glu Asn Val Leu Asn Lys Gly Leu Ala Leu Ile Ile Gly Glu Phe Gly 
    210                 215                 220                 


Gly Tyr His Thr Asn Gly Asp Val Asp Glu Tyr Ala Ile Met Arg Tyr 
225                 230                 235                 240 


Gly Gln Glu Leu Gly Val Gly Trp Leu Ala Trp Ser Trp Tyr Gly Asn 
                245                 250                 255     


Ser Asp Gly Leu Arg Tyr Leu Asp Met Ala Thr Gly Pro Asn Gly Ser 
            260                 265                 270         


Leu Thr Ser Phe Gly Asn Thr Val Val Asn Asp Thr Tyr Gly Ile Lys 
        275                 280                 285             


Asn Thr Ser Gln Lys Ala Gly Ile Phe 
    290                 295         


<210>  7
<211>  298
<212>  PRT
<213>  artificial sequence

<220>
<223>  variant

<400>  7

Met Ala Thr Gly Phe Tyr Val Ser Gly Thr Lys Leu Tyr Asp Ser Thr 
1               5                   10                  15      


Gly Lys Glu Phe Val Met Arg Gly Val Asn His Gly His Thr Trp Phe 
            20                  25                  30          


Lys Asn Asp Leu Asn Glu Ala Ile Pro Ala Ile Ala Lys Thr Gly Ala 
        35                  40                  45              


Asn Thr Val Arg Ile Val Leu Ser Asn Gly Val Gln Tyr Thr Arg Asp 
    50                  55                  60                  


Asp Leu Asp Ala Val Lys Asn Ile Ile Asn Val Val Asn Gln Asn Lys 
65                  70                  75                  80  


Met Ile Ala Val Leu Glu Val His Asp Ala Thr Gly Lys Asp Asp Tyr 
                85                  90                  95      


Asp Ser Leu Asp Ala Ala Val Asn Tyr Trp Ile Ser Ile Lys Glu Ala 
            100                 105                 110         


Leu Ile Gly Lys Glu Asp Arg Val Ile Val Asn Ile Ala Asn Glu Trp 
        115                 120                 125             


Met Gly Thr Trp Asn Gly Ser Ala Trp Ala Asp Gly Tyr Lys Gln Ala 
    130                 135                 140                 


Ile Pro Lys Leu Arg Asn Ala Gly Ile Lys Asn Thr Leu Ile Val Asp 
145                 150                 155                 160 


Ala Ala Gly Trp Gly Gln Phe Ser Gln Ser Ile Val Asp Tyr Gly Gln 
                165                 170                 175     


Ser Val Phe Ala Ala Asp Ser Leu Lys Asn Thr Val Phe Ser Ile His 
            180                 185                 190         


Met Tyr Glu Tyr Ala Gly Lys Asp Ala Ala Thr Val Lys Ala Asn Met 
        195                 200                 205             


Glu Asn Val Leu Asn Lys Gly Leu Ala Leu Ile Ile Gly Glu Phe Gly 
    210                 215                 220                 


Pro Tyr His Val Asn Gly Asp Val Asp Glu Leu Ala Ile Met Arg Tyr 
225                 230                 235                 240 


Gly Gln Glu Leu Gly Val Gly Trp Leu Ala Trp Ser Trp Tyr Gly Asn 
                245                 250                 255     


Ser Asp Gly Leu Arg Tyr Leu Asp Met Ala Thr Gly Pro Asn Gly Ser 
            260                 265                 270         


Leu Thr Ser Phe Gly Asn Thr Val Val Asn Asp Thr Tyr Gly Ile Lys 
        275                 280                 285             


Asn Thr Ser Gln Lys Ala Gly Ile Phe Gln 
    290                 295             


<210>  8
<211>  309
<212>  PRT
<213>  unknown

<220>
<223>  Bacillus sp.

<400>  8

Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr Asp Ala Asn 
1               5                   10                  15      


Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His Ala Trp Tyr 
            20                  25                  30          


Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn Thr Gly Ala 
        35                  40                  45              


Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp Thr Lys Asp 
    50                  55                  60                  


Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu Asp Asn His 
65                  70                  75                  80  


Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr Asp Ser Ile 
                85                  90                  95      


Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met Arg Ser Ala 
            100                 105                 110         


Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala Asn Glu Trp 
        115                 120                 125             


Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr Lys Gln Ala 
    130                 135                 140                 


Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu Met Val Asp 
145                 150                 155                 160 


Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp Tyr Gly Arg 
                165                 170                 175     


Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe Ser Ile His 
            180                 185                 190         


Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg Thr Asn Ile 
        195                 200                 205             


Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly Glu Phe Gly 
    210                 215                 220                 


His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile Met Ser Tyr 
225                 230                 235                 240 


Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp Lys Gly Asn 
                245                 250                 255     


Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp Ala Gly Asn 
            260                 265                 270         


Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro Tyr Gly Leu 
        275                 280                 285             


Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly Gly Ser Asp 
    290                 295                 300                 


Gly Gly Thr Ser Pro 
305                 


<210>  9
<211>  293
<212>  PRT
<213>  Bacillus agaradhaerens

<400>  9

Gly Phe Ser Val Asp Gly Asn Thr Leu Tyr Asp Ala Asn Gly Gln Pro 
1               5                   10                  15      


Phe Val Met Arg Gly Ile Asn His Gly His Ala Trp Tyr Lys Asp Thr 
            20                  25                  30          


Ala Ser Thr Ala Ile Pro Ala Ile Ala Glu Gln Gly Ala Asn Thr Ile 
        35                  40                  45              


Arg Ile Val Leu Ser Asp Gly Gly Gln Trp Glu Lys Asp Asp Ile Asp 
    50                  55                  60                  


Thr Ile Arg Glu Val Ile Glu Leu Ala Glu Gln Asn Lys Met Val Ala 
65                  70                  75                  80  


Val Val Glu Val His Asp Ala Thr Gly Arg Asp Ser Arg Ser Asp Leu 
                85                  90                  95      


Asn Arg Ala Val Asp Tyr Trp Ile Glu Met Lys Asp Ala Leu Ile Gly 
            100                 105                 110         


Lys Glu Asp Thr Val Ile Ile Asn Ile Ala Asn Glu Trp Tyr Gly Ser 
        115                 120                 125             


Trp Asp Gly Ser Ala Trp Ala Asp Gly Tyr Ile Asp Val Ile Pro Lys 
    130                 135                 140                 


Leu Arg Asp Ala Gly Leu Thr His Thr Leu Met Val Asp Ala Ala Gly 
145                 150                 155                 160 


Trp Gly Gln Tyr Pro Gln Ser Ile His Asp Tyr Gly Gln Asp Val Phe 
                165                 170                 175     


Asn Ala Asp Pro Leu Lys Asn Thr Met Phe Ser Ile His Met Tyr Glu 
            180                 185                 190         


Tyr Ala Gly Gly Asp Ala Asn Thr Val Arg Ser Asn Ile Asp Arg Val 
        195                 200                 205             


Ile Asp Gln Asp Leu Ala Leu Val Ile Gly Glu Phe Gly His Arg His 
    210                 215                 220                 


Thr Asp Val Asp Glu Asp Thr Ile Leu Ser Tyr Ser Glu Glu Thr Gly 
225                 230                 235                 240 


Thr Gly Trp Leu Ala Trp Ser Trp Lys Gly Asn Ser Thr Ser Trp Asp 
                245                 250                 255     


Tyr Leu Asp Leu Ser Glu Asp Trp Ala Gly Gln His Leu Thr Asp Trp 
            260                 265                 270         


Gly Asn Arg Ile Val His Gly Ala Asp Gly Leu Gln Glu Thr Ser Lys 
        275                 280                 285             


Pro Ser Thr Val Phe 
    290             


<210>  10
<211>  299
<212>  PRT
<213>  unknown

<220>
<223>  Bacillus sp. JAMB-602

<400>  10

Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr Asp Ala Asn Gly 
1               5                   10                  15      


Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His Ala Trp Tyr Lys 
            20                  25                  30          


Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn Thr Gly Ala Asn 
        35                  40                  45              


Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp Thr Lys Asp Asp 
    50                  55                  60                  


Ile Gln Thr Val Arg Asn Leu Ile Ser Leu Ala Glu Asp Asn Asn Leu 
65                  70                  75                  80  


Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr Asp Ser Ile Ala 
                85                  90                  95      


Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met Arg Ser Ala Leu 
            100                 105                 110         


Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala Asn Glu Trp Phe 
        115                 120                 125             


Gly Ser Trp Asp Gly Ala Ala Trp Ala Asp Gly Tyr Lys Gln Ala Ile 
    130                 135                 140                 


Pro Arg Leu Arg Asn Ala Gly Leu Asn Asn Thr Leu Met Ile Asp Ala 
145                 150                 155                 160 


Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp Tyr Gly Arg Glu 
                165                 170                 175     


Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe Ser Ile His Met 
            180                 185                 190         


Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg Thr Asn Ile Asp 
        195                 200                 205             


Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly Glu Phe Gly His 
    210                 215                 220                 


Arg His Thr Asn Gly Asp Val Asp Glu Ser Thr Ile Met Ser Tyr Ser 
225                 230                 235                 240 


Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp Lys Gly Asn Gly 
                245                 250                 255     


Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp Ala Gly Asn Asn 
            260                 265                 270         


Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro Tyr Gly Leu Arg 
        275                 280                 285             


Glu Thr Ser Lys Leu Ser Thr Val Phe Thr Gly 
    290                 295                 


<210>  11
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  Motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  X is F or Y

<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  X is any amino acid

<400>  11

Trp Xaa Lys Asn Asp Leu Xaa Xaa Ala Ile 
1               5                   10  


<210>  12
<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  motif


<220>
<221>  MISC_FEATURE
<222>  (3)..(5)
<223>  X is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  X is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  X is any amino acid

<400>  12

Leu Asp Xaa Xaa Xaa Gly Pro Xaa Gly Xaa Leu Thr 
1               5                   10          


<210>  13
<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  motif


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X is M or L

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  X is V or A

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  X is T or A

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  X is N, A or S

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  X is N, S or T

<400>  13

Leu Asp Xaa Xaa Xaa Gly Pro Xaa Gly Xaa Leu Thr 
1               5                   10          


<210>  14
<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  motif


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X is M or L

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  X is N or A

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  X is S or T

<400>  14

Leu Asp Xaa Ala Thr Gly Pro Xaa Gly Xaa Leu Thr 
1               5                   10          


<210>  15
<211>  299
<212>  PRT
<213>  unknown

<220>
<223>  Bacillus sp.

<400>  15

Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr Asp Ala Asn 
1               5                   10                  15      


Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His Ala Trp Tyr 
            20                  25                  30          


Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn Thr Gly Ala 
        35                  40                  45              


Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp Thr Lys Asp 
    50                  55                  60                  


Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu Asp Asn His 
65                  70                  75                  80  


Leu Val Ala Val Pro Glu Val His Asp Ala Thr Gly Tyr Asp Ser Ile 
                85                  90                  95      


Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met Arg Ser Ala 
            100                 105                 110         


Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala Asn Glu Trp 
        115                 120                 125             


Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr Lys Gln Ala 
    130                 135                 140                 


Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu Met Val Asp 
145                 150                 155                 160 


Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp Tyr Gly Arg 
                165                 170                 175     


Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe Ser Ile His 
            180                 185                 190         


Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg Thr Asn Ile 
        195                 200                 205             


Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly Glu Phe Gly 
    210                 215                 220                 


His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile Met Ser Tyr 
225                 230                 235                 240 


Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp Lys Gly Asn 
                245                 250                 255     


Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp Ala Gly Asn 
            260                 265                 270         


Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro Tyr Gly Leu 
        275                 280                 285             


Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr 
    290                 295                 


<210>  16
<211>  312
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  16

Ala Gly Lys Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr 
1               5                   10                  15      


Asp Ala Asn Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His 
            20                  25                  30          


Ala Trp Tyr Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn 
        35                  40                  45              


Thr Gly Ala Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp 
    50                  55                  60                  


Thr Lys Asp Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu 
65                  70                  75                  80  


Asp Asn His Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr 
                85                  90                  95      


Asp Ser Ile Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met 
            100                 105                 110         


Arg Ser Ala Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala 
        115                 120                 125             


Asn Glu Trp Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr 
    130                 135                 140                 


Lys Gln Ala Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu 
145                 150                 155                 160 


Met Val Asp Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp 
                165                 170                 175     


Tyr Gly Arg Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe 
            180                 185                 190         


Ser Ile His Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg 
        195                 200                 205             


Thr Asn Ile Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly 
    210                 215                 220                 


Glu Phe Gly His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile 
225                 230                 235                 240 


Met Ser Tyr Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp 
                245                 250                 255     


Lys Gly Asn Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp 
            260                 265                 270         


Ala Gly Asn Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro 
        275                 280                 285             


Tyr Gly Leu Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly 
    290                 295                 300                 


Gly Ser Asp Gly Gly Thr Ser Pro 
305                 310         


<210>  17
<211>  309
<212>  PRT
<213>  unknown

<220>
<223>  Bacillus sp.

<400>  17

Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr Asp Ala Asn 
1               5                   10                  15      


Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His Ala Trp Tyr 
            20                  25                  30          


Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn Thr Gly Ala 
        35                  40                  45              


Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp Thr Lys Asp 
    50                  55                  60                  


Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu Asp Asn His 
65                  70                  75                  80  


Leu Val Ala Val Pro Glu Val His Asp Ala Thr Gly Tyr Asp Ser Ile 
                85                  90                  95      


Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met Arg Ser Ala 
            100                 105                 110         


Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala Asn Glu Trp 
        115                 120                 125             


Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr Lys Gln Ala 
    130                 135                 140                 


Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu Met Val Asp 
145                 150                 155                 160 


Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp Tyr Gly Arg 
                165                 170                 175     


Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe Ser Ile His 
            180                 185                 190         


Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg Thr Asn Ile 
        195                 200                 205             


Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly Glu Phe Gly 
    210                 215                 220                 


His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile Met Ser Tyr 
225                 230                 235                 240 


Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp Lys Gly Asn 
                245                 250                 255     


Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp Ala Gly Asn 
            260                 265                 270         


Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro Tyr Gly Leu 
        275                 280                 285             


Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly Gly Ser Asp 
    290                 295                 300                 


Gly Gly Thr Ser Pro 
305                 


<210>  18
<211>  1023
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic

<400>  18
gtgagaagca aaaaattgtg gatcagcttg ttgtttgcgt taacgttaat ctttacgatg       60

gcgttcagca acatgagcgc gcaggctgct ggaaaagcaa actcaggctt ttatgtttca      120

ggcacaacac tgtacgacgc aaatggcaat ccgtttgtta tgagaggcat taaccatgga      180

catgcctggt ataaagatca agcaacaaca gcgattgaag gcattgcaaa tacaggcgca      240

aacacagtga gaattgttct ttcagacgga ggccaatgga caaaagatga tattcatacg      300

gttagaaatc tgatttcact ggcagaggat aatcatctgg ttgcagttct ggaagttcat      360

gatgcaacag gctatgattc aattgcaagc cttaatagag cagttgatta ttggattgaa      420

atgagatcag cactgattgg caaagaagat acagtcatca ttaacattgc caacgaatgg      480

tttggctcat gggaaggcga tgcatgggca gacggctata agcaagcaat tccgagactg      540

agaaatgcag gacttaatca tacactgatg gttgatgcag caggctgggg ccagttcccg      600

caatcaattc acgattatgg aagagaggtt tttaacgcag atccgcagag aaatacaatg      660

tttagcattc atatgtacga atatgcaggc ggcaacgcat cacaagttag aacaaatatt      720

gatagagttc tgaaccagga tctggcactg gttatcggag aatttggcca tagacatacg      780

aacggcgatg ttgatgaagc tacaattatg tcatactcag aacagagagg cgtcggatgg      840

ctggcatggt catggaaagg caacggaccg gaatgggaat atctggatct gtcaaatgat      900

tgggcaggaa ataacctgac agcatggggc aatacgattg ttaacggccc gtatggcctg      960

agagaaacat caagactgtc aacagttttt acaggaggcg gatcagatgg cggcacatca     1020

ccg                                                                   1023


<210>  19
<211>  341
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  19

Met Arg Ser Lys Lys Leu Trp Ile Ser Leu Leu Phe Ala Leu Thr Leu 
1               5                   10                  15      


Ile Phe Thr Met Ala Phe Ser Asn Met Ser Ala Gln Ala Ala Gly Lys 
            20                  25                  30          


Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr Asp Ala Asn 
        35                  40                  45              


Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His Ala Trp Tyr 
    50                  55                  60                  


Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn Thr Gly Ala 
65                  70                  75                  80  


Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp Thr Lys Asp 
                85                  90                  95      


Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu Asp Asn His 
            100                 105                 110         


Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr Asp Ser Ile 
        115                 120                 125             


Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met Arg Ser Ala 
    130                 135                 140                 


Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala Asn Glu Trp 
145                 150                 155                 160 


Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr Lys Gln Ala 
                165                 170                 175     


Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu Met Val Asp 
            180                 185                 190         


Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp Tyr Gly Arg 
        195                 200                 205             


Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe Ser Ile His 
    210                 215                 220                 


Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg Thr Asn Ile 
225                 230                 235                 240 


Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly Glu Phe Gly 
                245                 250                 255     


His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile Met Ser Tyr 
            260                 265                 270         


Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp Lys Gly Asn 
        275                 280                 285             


Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp Ala Gly Asn 
    290                 295                 300                 


Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro Tyr Gly Leu 
305                 310                 315                 320 


Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly Gly Ser Asp 
                325                 330                 335     


Gly Gly Thr Ser Pro 
            340     


<210>  20
<211>  312
<212>  PRT
<213>  artificial sequence

<220>
<223>  BspMan5 variant

<400>  20

Ala Gly Lys Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr 
1               5                   10                  15      


Asp Ala Asn Gly Asn Glu Phe Val Met Arg Gly Ile Asn His Gly His 
            20                  25                  30          


Ala Trp Tyr Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn 
        35                  40                  45              


Thr Gly Ala Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp 
    50                  55                  60                  


Thr Lys Asp Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu 
65                  70                  75                  80  


Asp Asn His Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr 
                85                  90                  95      


Asp Ser Ile Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met 
            100                 105                 110         


Arg Ser Ala Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala 
        115                 120                 125             


Asn Glu Trp Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr 
    130                 135                 140                 


Lys Gln Ala Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu 
145                 150                 155                 160 


Met Val Asp Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp 
                165                 170                 175     


Tyr Gly Arg Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe 
            180                 185                 190         


Ser Ile His Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg 
        195                 200                 205             


Thr Asn Ile Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly 
    210                 215                 220                 


Glu Phe Gly His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile 
225                 230                 235                 240 


Met Ser Tyr Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp 
                245                 250                 255     


Lys Gly Asn Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp 
            260                 265                 270         


Ala Gly Asn Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro 
        275                 280                 285             


Tyr Gly Leu Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly 
    290                 295                 300                 


Gly Ser Asp Gly Gly Thr Ser Pro 
305                 310         


<210>  21
<211>  312
<212>  PRT
<213>  artificial sequence

<220>
<223>  BspMan5 variant

<400>  21

Ala Gly Lys Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr 
1               5                   10                  15      


Asp Ala Asn Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His 
            20                  25                  30          


Ala Trp Tyr Lys Asp Gln Ala Thr Glu Ala Ile Glu Gly Ile Ala Asn 
        35                  40                  45              


Thr Gly Ala Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp 
    50                  55                  60                  


Thr Lys Asp Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu 
65                  70                  75                  80  


Asp Asn His Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr 
                85                  90                  95      


Asp Ser Ile Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met 
            100                 105                 110         


Arg Ser Ala Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala 
        115                 120                 125             


Asn Glu Trp Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr 
    130                 135                 140                 


Lys Gln Ala Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu 
145                 150                 155                 160 


Met Val Asp Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp 
                165                 170                 175     


Tyr Gly Arg Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe 
            180                 185                 190         


Ser Ile His Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg 
        195                 200                 205             


Thr Asn Ile Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly 
    210                 215                 220                 


Glu Phe Gly His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile 
225                 230                 235                 240 


Met Ser Tyr Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp 
                245                 250                 255     


Lys Gly Asn Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp 
            260                 265                 270         


Ala Gly Asn Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro 
        275                 280                 285             


Tyr Gly Leu Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly 
    290                 295                 300                 


Gly Ser Asp Gly Gly Thr Ser Pro 
305                 310         


<210>  22
<211>  312
<212>  PRT
<213>  artificial sequence

<220>
<223>  BspMan5 variant

<400>  22

Ala Gly Lys Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr 
1               5                   10                  15      


Asp Ala Asn Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His 
            20                  25                  30          


Ala Trp Tyr Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn 
        35                  40                  45              


Thr Gly Ala Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp 
    50                  55                  60                  


Thr Lys Asp Asp Ile Asp Thr Val Arg Asn Leu Ile Ser Leu Ala Glu 
65                  70                  75                  80  


Asp Asn His Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr 
                85                  90                  95      


Asp Ser Ile Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met 
            100                 105                 110         


Arg Ser Ala Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala 
        115                 120                 125             


Asn Glu Trp Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr 
    130                 135                 140                 


Lys Gln Ala Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu 
145                 150                 155                 160 


Met Val Asp Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp 
                165                 170                 175     


Tyr Gly Arg Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe 
            180                 185                 190         


Ser Ile His Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg 
        195                 200                 205             


Thr Asn Ile Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly 
    210                 215                 220                 


Glu Phe Gly His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile 
225                 230                 235                 240 


Met Ser Tyr Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp 
                245                 250                 255     


Lys Gly Asn Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp 
            260                 265                 270         


Ala Gly Asn Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro 
        275                 280                 285             


Tyr Gly Leu Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly 
    290                 295                 300                 


Gly Ser Asp Gly Gly Thr Ser Pro 
305                 310         


<210>  23
<211>  312
<212>  PRT
<213>  artificial sequence

<220>
<223>  BspMan5 variant

<400>  23

Ala Gly Lys Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr 
1               5                   10                  15      


Asp Ala Asn Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His 
            20                  25                  30          


Ala Trp Tyr Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn 
        35                  40                  45              


Thr Gly Ala Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp 
    50                  55                  60                  


Thr Lys Asp Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu 
65                  70                  75                  80  


Asp Asn His Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr 
                85                  90                  95      


Asp Ser Ile Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met 
            100                 105                 110         


Arg Ser Ala Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala 
        115                 120                 125             


Asn Glu Trp Met Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr 
    130                 135                 140                 


Lys Gln Ala Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu 
145                 150                 155                 160 


Met Val Asp Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp 
                165                 170                 175     


Tyr Gly Arg Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe 
            180                 185                 190         


Ser Ile His Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg 
        195                 200                 205             


Thr Asn Ile Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly 
    210                 215                 220                 


Glu Phe Gly His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile 
225                 230                 235                 240 


Met Ser Tyr Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp 
                245                 250                 255     


Lys Gly Asn Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp 
            260                 265                 270         


Ala Gly Asn Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro 
        275                 280                 285             


Tyr Gly Leu Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly 
    290                 295                 300                 


Gly Ser Asp Gly Gly Thr Ser Pro 
305                 310         


<210>  24
<211>  312
<212>  PRT
<213>  artificial sequence

<220>
<223>  BspMan5 variant

<400>  24

Ala Gly Lys Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr 
1               5                   10                  15      


Asp Ala Asn Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His 
            20                  25                  30          


Ala Trp Tyr Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn 
        35                  40                  45              


Thr Gly Ala Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp 
    50                  55                  60                  


Thr Lys Asp Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu 
65                  70                  75                  80  


Asp Asn His Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr 
                85                  90                  95      


Asp Ser Ile Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met 
            100                 105                 110         


Arg Ser Ala Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala 
        115                 120                 125             


Asn Glu Trp Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr 
    130                 135                 140                 


Lys Gln Ala Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu 
145                 150                 155                 160 


Met Val Asp Ala Ala Gly Trp Gly Gln Phe Ser Gln Ser Ile His Asp 
                165                 170                 175     


Tyr Gly Arg Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe 
            180                 185                 190         


Ser Ile His Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg 
        195                 200                 205             


Thr Asn Ile Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly 
    210                 215                 220                 


Glu Phe Gly His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile 
225                 230                 235                 240 


Met Ser Tyr Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp 
                245                 250                 255     


Lys Gly Asn Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp 
            260                 265                 270         


Ala Gly Asn Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro 
        275                 280                 285             


Tyr Gly Leu Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly 
    290                 295                 300                 


Gly Ser Asp Gly Gly Thr Ser Pro 
305                 310         


<210>  25
<211>  312
<212>  PRT
<213>  artificial sequence

<220>
<223>  BspMan5 variant

<400>  25

Ala Gly Lys Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr 
1               5                   10                  15      


Asp Ala Asn Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His 
            20                  25                  30          


Ala Trp Tyr Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn 
        35                  40                  45              


Thr Gly Ala Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp 
    50                  55                  60                  


Thr Lys Asp Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu 
65                  70                  75                  80  


Asp Asn His Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr 
                85                  90                  95      


Asp Ser Ile Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met 
            100                 105                 110         


Arg Ser Ala Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala 
        115                 120                 125             


Asn Glu Trp Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr 
    130                 135                 140                 


Lys Gln Ala Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu 
145                 150                 155                 160 


Met Val Asp Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp 
                165                 170                 175     


Tyr Gly Arg Glu Val Phe Asn Ala Asp Pro Leu Arg Asn Thr Met Phe 
            180                 185                 190         


Ser Ile His Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg 
        195                 200                 205             


Thr Asn Ile Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly 
    210                 215                 220                 


Glu Phe Gly His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile 
225                 230                 235                 240 


Met Ser Tyr Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp 
                245                 250                 255     


Lys Gly Asn Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp 
            260                 265                 270         


Ala Gly Asn Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro 
        275                 280                 285             


Tyr Gly Leu Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly 
    290                 295                 300                 


Gly Ser Asp Gly Gly Thr Ser Pro 
305                 310         


<210>  26
<211>  312
<212>  PRT
<213>  artificial sequence

<220>
<223>  BspMan5 variant

<400>  26

Ala Gly Lys Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr 
1               5                   10                  15      


Asp Ala Asn Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His 
            20                  25                  30          


Ala Trp Tyr Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn 
        35                  40                  45              


Thr Gly Ala Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp 
    50                  55                  60                  


Thr Lys Asp Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu 
65                  70                  75                  80  


Asp Asn His Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr 
                85                  90                  95      


Asp Ser Ile Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met 
            100                 105                 110         


Arg Ser Ala Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala 
        115                 120                 125             


Asn Glu Trp Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr 
    130                 135                 140                 


Lys Gln Ala Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu 
145                 150                 155                 160 


Met Val Asp Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp 
                165                 170                 175     


Tyr Gly Arg Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe 
            180                 185                 190         


Ser Ile His Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg 
        195                 200                 205             


Thr Asn Ile Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly 
    210                 215                 220                 


Glu Phe Gly Pro Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile 
225                 230                 235                 240 


Met Ser Tyr Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp 
                245                 250                 255     


Lys Gly Asn Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp 
            260                 265                 270         


Ala Gly Asn Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro 
        275                 280                 285             


Tyr Gly Leu Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly 
    290                 295                 300                 


Gly Ser Asp Gly Gly Thr Ser Pro 
305                 310         


<210>  27
<211>  312
<212>  PRT
<213>  artificial sequence

<220>
<223>  BspMan5 variant

<400>  27

Ala Gly Lys Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr 
1               5                   10                  15      


Asp Ala Asn Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His 
            20                  25                  30          


Ala Trp Tyr Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn 
        35                  40                  45              


Thr Gly Ala Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp 
    50                  55                  60                  


Thr Lys Asp Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu 
65                  70                  75                  80  


Asp Asn His Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr 
                85                  90                  95      


Asp Ser Ile Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met 
            100                 105                 110         


Arg Ser Ala Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala 
        115                 120                 125             


Asn Glu Trp Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr 
    130                 135                 140                 


Lys Gln Ala Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu 
145                 150                 155                 160 


Met Val Asp Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp 
                165                 170                 175     


Tyr Gly Arg Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe 
            180                 185                 190         


Ser Ile His Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg 
        195                 200                 205             


Thr Asn Ile Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly 
    210                 215                 220                 


Glu Phe Gly His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile 
225                 230                 235                 240 


Met Ser Tyr Ser Glu Gln Leu Gly Val Gly Trp Leu Ala Trp Ser Trp 
                245                 250                 255     


Lys Gly Asn Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp 
            260                 265                 270         


Ala Gly Asn Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro 
        275                 280                 285             


Tyr Gly Leu Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly 
    290                 295                 300                 


Gly Ser Asp Gly Gly Thr Ser Pro 
305                 310         


<210>  28
<211>  312
<212>  PRT
<213>  artificial sequence

<220>
<223>  BspMan5 variant

<400>  28

Ala Gly Lys Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr 
1               5                   10                  15      


Asp Ala Asn Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His 
            20                  25                  30          


Ala Trp Tyr Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn 
        35                  40                  45              


Thr Gly Ala Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp 
    50                  55                  60                  


Thr Lys Asp Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu 
65                  70                  75                  80  


Asp Asn His Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr 
                85                  90                  95      


Asp Ser Ile Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met 
            100                 105                 110         


Arg Ser Ala Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala 
        115                 120                 125             


Asn Glu Trp Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr 
    130                 135                 140                 


Lys Gln Ala Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu 
145                 150                 155                 160 


Met Val Asp Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp 
                165                 170                 175     


Tyr Gly Arg Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe 
            180                 185                 190         


Ser Ile His Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg 
        195                 200                 205             


Thr Asn Ile Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly 
    210                 215                 220                 


Glu Phe Gly His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile 
225                 230                 235                 240 


Met Ser Tyr Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp 
                245                 250                 255     


Lys Gly Asn Gly Asp Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp 
            260                 265                 270         


Ala Gly Asn Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro 
        275                 280                 285             


Tyr Gly Leu Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly 
    290                 295                 300                 


Gly Ser Asp Gly Gly Thr Ser Pro 
305                 310         


<210>  29
<211>  312
<212>  PRT
<213>  artificial sequence

<220>
<223>  BspMan5 variant

<400>  29

Ala Gly Lys Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr 
1               5                   10                  15      


Asp Ala Asn Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His 
            20                  25                  30          


Ala Trp Tyr Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn 
        35                  40                  45              


Thr Gly Ala Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp 
    50                  55                  60                  


Thr Lys Asp Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu 
65                  70                  75                  80  


Asp Asn His Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr 
                85                  90                  95      


Asp Ser Ile Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met 
            100                 105                 110         


Arg Ser Ala Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala 
        115                 120                 125             


Asn Glu Trp Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr 
    130                 135                 140                 


Lys Gln Ala Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu 
145                 150                 155                 160 


Met Val Asp Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp 
                165                 170                 175     


Tyr Gly Arg Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe 
            180                 185                 190         


Ser Ile His Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg 
        195                 200                 205             


Thr Asn Ile Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly 
    210                 215                 220                 


Glu Phe Gly His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile 
225                 230                 235                 240 


Met Ser Tyr Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp 
                245                 250                 255     


Lys Gly Asn Gly Pro Glu Trp Arg Tyr Leu Asp Leu Ser Asn Asp Trp 
            260                 265                 270         


Ala Gly Asn Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro 
        275                 280                 285             


Tyr Gly Leu Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly 
    290                 295                 300                 


Gly Ser Asp Gly Gly Thr Ser Pro 
305                 310         


