                         SEQUENCE LISTING

<110>  Commissariat  l'nergie atomique
 
<120>  Modified magnetotactic bacteria expressing a metallophore 
       specific for cobalt and/or nickel

<130>  F263/625 WO - ELMA/safl

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  273
<212>  PRT
<213>  Staphylococcus aureus


<220>
<221>  MISC_FEATURE
<222>  (1)..(273)
<223>  diaminopimelate epimerase (SAV2470)

<400>  1

Met Asn Arg Gln Val Ile Glu Phe Ser Lys Tyr Asn Pro Ser Gly Asn 
1               5                   10                  15      


Met Thr Ile Leu Val His Ser Lys His Asp Ala Ser Glu Tyr Ala Ser 
            20                  25                  30          


Ile Ala Asn Gln Leu Met Ala Ala Thr His Val Cys Cys Glu Gln Val 
        35                  40                  45              


Gly Phe Ile Glu Ser Thr Gln Asn Asp Asp Gly Asn Asp Phe His Leu 
    50                  55                  60                  


Val Met Ser Gly Asn Glu Phe Cys Gly Asn Ala Thr Met Ser Tyr Ile 
65                  70                  75                  80  


His His Leu Gln Glu Ser His Leu Leu Lys Asp Gln Gln Phe Lys Val 
                85                  90                  95      


Lys Val Ser Gly Cys Ser Asp Leu Val Gln Cys Ala Ile His Asp Cys 
            100                 105                 110         


Gln Tyr Tyr Glu Val Gln Met Pro Gln Ala His Arg Val Val Pro Thr 
        115                 120                 125             


Thr Ile Asn Met Gly Asn His Ser Trp Lys Ala Leu Glu Ile Ile Tyr 
    130                 135                 140                 


Glu Thr Tyr Val His Tyr Val Ile Pro Val Lys Gln Val Thr Thr Glu 
145                 150                 155                 160 


Ile Gln His Leu Val Glu Ala Phe Val Arg Glu Gln Gln Trp Ser His 
                165                 170                 175     


Lys Tyr Lys Thr Val Gly Met Met Leu Phe Asp Glu Gln Arg Gln Phe 
            180                 185                 190         


Leu Gln Pro Leu Ile Tyr Ile Pro Glu Ile Gln Ser Leu Ile Trp Glu 
        195                 200                 205             


Asn Ser Cys Gly Ser Gly Thr Ala Ser Ile Gly Val Phe Asn Asn Tyr 
    210                 215                 220                 


Gln Arg Asn Asp Ala Cys Lys Asp Phe Thr Val His Gln Pro Gly Gly 
225                 230                 235                 240 


Ser Ile Leu Val Thr Ser Lys Arg Cys His Gln Leu Gly Tyr Gln Thr 
                245                 250                 255     


Ser Ile Lys Gly Gln Val Thr Thr Val Ala Thr Gly Lys Ala Tyr Ile 
            260                 265                 270         


Glu 
    


<210>  2
<211>  272
<212>  PRT
<213>  Staphylococcus aureus


<220>
<221>  MISC_FEATURE
<222>  (1)..(272)
<223>  supposed dehydrogenase (SAV2469)

<400>  2

Met Asn Asn Phe Asn Asn Glu Ile Lys Leu Ile Leu Gln Gln Tyr Leu 
1               5                   10                  15      


Glu Lys Phe Glu Ala His Tyr Glu Arg Val Leu Gln Asp Asp Gln Tyr 
            20                  25                  30          


Ile Glu Ala Leu Glu Thr Leu Met Asp Asp Tyr Ser Glu Phe Ile Leu 
        35                  40                  45              


Asn Pro Ile Tyr Glu Gln Gln Phe Asn Ala Trp Arg Asp Val Glu Glu 
    50                  55                  60                  


Lys Ala Gln Leu Ile Lys Ser Leu Gln Tyr Ile Thr Ala Gln Cys Val 
65                  70                  75                  80  


Lys Gln Val Glu Val Ile Arg Ala Arg Arg Leu Leu Asp Gly Gln Ala 
                85                  90                  95      


Ser Thr Thr Gly Tyr Phe Asp Asn Ile Glu His Cys Ile Asp Glu Glu 
            100                 105                 110         


Phe Gly Gln Cys Ser Ile Thr Ser Asn Asp Lys Leu Leu Leu Val Gly 
        115                 120                 125             


Ser Gly Ala Tyr Pro Met Thr Leu Ile Gln Val Ala Lys Glu Thr Gly 
    130                 135                 140                 


Ala Ser Val Ile Gly Ile Asp Ile Asp Pro Gln Ala Val Asp Leu Gly 
145                 150                 155                 160 


Arg Arg Ile Val Asn Val Leu Ala Pro Asn Glu Asp Ile Thr Ile Thr 
                165                 170                 175     


Asp Gln Lys Val Ser Glu Leu Lys Asp Ile Lys Asp Val Thr His Ile 
            180                 185                 190         


Ile Phe Ser Ser Thr Ile Pro Leu Lys Tyr Ser Ile Leu Glu Glu Leu 
        195                 200                 205             


Tyr Asp Leu Thr Asn Glu Asn Val Val Val Ala Met Arg Phe Gly Asp 
    210                 215                 220                 


Gly Ile Lys Ala Ile Phe Asn Tyr Pro Ser Gln Glu Thr Ala Glu Asp 
225                 230                 235                 240 


Lys Trp Gln Cys Val Asn Lys His Met Arg Pro Gln Gln Ile Phe Asp 
                245                 250                 255     


Ile Ala Leu Tyr Lys Lys Ala Ala Ile Lys Val Gly Ile Thr Asp Val 
            260                 265                 270         


<210>  3
<211>  433
<212>  PRT
<213>  Staphylococcus aureus


<220>
<221>  MISC_FEATURE
<222>  (1)..(433)
<223>  DUF2338 (SAV2468)

<400>  3

Met Ser Lys Leu Leu Met Ile Gly Thr Gly Pro Val Ala Ile Gln Leu 
1               5                   10                  15      


Ala Asn Ile Cys Tyr Leu Lys Ser Asp Tyr Glu Ile Asp Met Val Gly 
            20                  25                  30          


Arg Ala Ser Thr Ser Glu Lys Ser Lys Arg Leu Tyr Gln Ala Tyr Lys 
        35                  40                  45              


Lys Glu Lys Gln Phe Glu Val Lys Ile Gln Asn Glu Ala His Gln His 
    50                  55                  60                  


Leu Glu Gly Lys Phe Glu Ile Asn Arg Leu Tyr Lys Asp Val Lys Asn 
65                  70                  75                  80  


Val Lys Gly Glu Tyr Glu Thr Val Val Met Ala Cys Thr Ala Asp Ala 
                85                  90                  95      


Tyr Tyr Asp Thr Leu Gln Gln Leu Ser Leu Glu Thr Leu Gln Ser Val 
            100                 105                 110         


Lys His Val Ile Leu Ile Ser Pro Thr Phe Gly Ser Gln Met Ile Val 
        115                 120                 125             


Glu Gln Phe Met Ser Lys Phe Ser Gln Asp Ile Glu Val Ile Ser Phe 
    130                 135                 140                 


Ser Thr Tyr Leu Gly Asp Thr Arg Ile Val Asp Lys Glu Ala Pro Asn 
145                 150                 155                 160 


His Val Leu Thr Thr Gly Val Lys Lys Lys Leu Tyr Met Gly Ser Thr 
                165                 170                 175     


His Ser Asn Ser Thr Met Cys Gln Arg Ile Ser Ala Leu Ala Glu Gln 
            180                 185                 190         


Leu Lys Ile Gln Leu Glu Val Val Glu Ser Pro Leu His Ala Glu Thr 
        195                 200                 205             


Arg Asn Ser Ser Leu Tyr Val His Pro Pro Leu Phe Met Asn Asp Phe 
    210                 215                 220                 


Ser Leu Lys Ala Ile Phe Glu Gly Thr Asp Val Pro Val Tyr Val Tyr 
225                 230                 235                 240 


Lys Leu Phe Pro Glu Gly Pro Ile Thr Met Thr Leu Ile Arg Glu Met 
                245                 250                 255     


Arg Leu Met Trp Lys Glu Met Met Ala Ile Leu Gln Ala Phe Arg Val 
            260                 265                 270         


Pro Ser Val Asn Leu Leu Gln Phe Met Val Lys Glu Asn Tyr Pro Val 
        275                 280                 285             


Arg Pro Glu Thr Leu Asp Glu Gly Asp Ile Glu His Phe Glu Ile Leu 
    290                 295                 300                 


Pro Asp Ile Leu Gln Glu Tyr Leu Leu Tyr Val Arg Tyr Thr Ala Ile 
305                 310                 315                 320 


Leu Ile Asp Pro Phe Ser Gln Pro Asp Glu Asn Gly His Tyr Phe Asp 
                325                 330                 335     


Phe Ser Ala Val Pro Phe Lys Gln Val Tyr Lys Asn Glu Gln Asp Val 
            340                 345                 350         


Val Gln Ile Pro Arg Met Pro Ser Glu Asp Tyr Tyr Arg Thr Ala Met 
        355                 360                 365             


Ile Gln His Ile Gly Lys Met Leu Gly Ile Lys Thr Pro Met Ile Asp 
    370                 375                 380                 


Gln Phe Leu Thr Arg Tyr Glu Ala Ser Cys Gln Ala Tyr Lys Asp Met 
385                 390                 395                 400 


His Gln Asp Gln Gln Leu Ser Ser Gln Phe Asn Thr Asn Leu Phe Glu 
                405                 410                 415     


Gly Asp Lys Ala Leu Val Thr Lys Phe Leu Glu Ile Asn Arg Thr Leu 
            420                 425                 430         


Ser 
    


<210>  4
<211>  263
<212>  PRT
<213>  Pseudomonas aeruginosa


<220>
<221>  MISC_FEATURE
<222>  (1)..(263)
<223>  PA4836

<400>  4

Met Gln Gly Arg Thr Pro Leu Leu Glu Thr Leu Arg Glu Leu Glu Cys 
1               5                   10                  15      


Glu Ile Arg Leu Leu Thr Val Tyr Ala Arg Glu Cys Cys Gly Cys Tyr 
            20                  25                  30          


Glu Ile Leu Arg Arg Lys Leu Asp Arg Leu Ser Gly Leu Ile Gly Glu 
        35                  40                  45              


Asp Cys Ser Arg Ala Gln Trp Gln Ala Asp Ser Asp Asp Pro Ala Leu 
    50                  55                  60                  


Gln Ala Leu Gly Leu Arg Leu Arg Asp Ala Ala Val Gln Ala Leu Cys 
65                  70                  75                  80  


Glu Leu Glu Lys His Leu Cys Gln Gly Val Leu His Glu Pro Gly Glu 
                85                  90                  95      


Met Gly Arg Tyr Leu Gly Ser Leu Leu Glu Ser Ile Arg Gly Glu Leu 
            100                 105                 110         


Asp Ser Ala Gly Ile Asp Ala Asp Ala Arg Val Leu Phe Val Gly Ser 
        115                 120                 125             


Gly Ala Leu Pro Thr Ser Ala Leu Val Leu Ala Arg Glu Val Gly Ala 
    130                 135                 140                 


His Leu Cys Cys Leu Asp Ile Asp Glu Glu Ala Leu Gly Cys Ala Arg 
145                 150                 155                 160 


Glu Ile Ala Arg Cys Gln Gly Leu Glu Ala Arg Met Gln Phe Ser Ser 
                165                 170                 175     


Leu Pro Pro Ala Glu Leu Ala Phe Ser Arg Asp Ala Thr His Phe Leu 
            180                 185                 190         


Ile Ala Ser Leu Val Gln Gln Lys Ser Ala Val Leu Ala Gln Ile Arg 
        195                 200                 205             


Gln Val Met Arg Ala Asp Ala Lys Val Leu Leu Arg His Gly Ser Gly 
    210                 215                 220                 


Ile Lys Gly Leu Phe Asn Tyr Pro Val Glu Pro Ala Glu Leu Asp Gly 
225                 230                 235                 240 


Trp Arg Val Cys Ala Glu Arg Val Ser Gln Pro Leu Tyr Asp Thr Leu 
                245                 250                 255     


Ile Leu Glu Lys Ala Gly Arg 
            260             


<210>  5
<211>  433
<212>  PRT
<213>  Pseudomonas aeruginosa


<220>
<221>  MISC_FEATURE
<222>  (1)..(433)
<223>  PA4835

<400>  5

Met Asn Ala Ala Asp Glu Ser Leu Gly Asn Val Leu Leu Val Gly Leu 
1               5                   10                  15      


Gly Ala Val Ala Ile Gln Val Ala Leu Asp Leu Arg Arg His Gly Ala 
            20                  25                  30          


Gly Arg Leu Gly Ala Leu Asn His Pro Gly Arg Arg Ser Gln Arg Ile 
        35                  40                  45              


Ala Glu Ala Leu Ala Arg Gly Ala Cys Leu Gln Leu Glu Gly Gln Gly 
    50                  55                  60                  


Gln His Arg Trp Leu Ser Gly Asn Ala Ala Leu Asp Val Phe His Gln 
65                  70                  75                  80  


Asp Pro Ala Glu Leu Arg Asp Asp Trp Gln Thr Leu Val Leu Cys Val 
                85                  90                  95      


Pro Ala Asp Ser Tyr Leu Asp Val Val Arg Gly Leu Pro Trp Glu Arg 
            100                 105                 110         


Leu Gly Gly Val Arg Thr Leu Leu Leu Val Ser Ala Phe Ile Gly Ala 
        115                 120                 125             


Asn Leu Leu Val Arg Ser Ala Leu Pro Ala Gly Cys Gln Ala Thr Val 
    130                 135                 140                 


Leu Ser Leu Ser Ser Tyr Tyr Ala Ala Thr Lys Val Ile Asp Glu Thr 
145                 150                 155                 160 


Gln Pro Leu Arg Ala Leu Thr Lys Ala Val Lys Arg Arg Val Tyr Leu 
                165                 170                 175     


Gly Ser Ser Arg Pro Asp Cys Pro Ala Arg Glu Thr Trp Arg Arg Val 
            180                 185                 190         


Leu Ala Gly Ser Gly Val Glu Val Val Pro Leu Ala Thr Pro Glu Ala 
        195                 200                 205             


Ala Glu Gly Arg Asn Val Thr Thr Tyr Val His Ser Pro Phe Phe Leu 
    210                 215                 220                 


Gly Glu Phe Ala Leu Ala Arg Ile Leu Ser Glu Gln Gly Pro Pro Gly 
225                 230                 235                 240 


Phe Met Tyr Lys Leu Tyr Pro Glu Gly Pro Ile Thr Pro Gly Ala Ile 
                245                 250                 255     


Gly Ala Met Arg Arg Leu Trp Cys Glu Leu Ser Glu Leu Leu Arg Arg 
            260                 265                 270         


Met Gly Ala Glu Pro Leu Asn Leu Leu Arg Phe Leu Asn Asp Asp Asn 
        275                 280                 285             


Tyr Pro Val His Glu Thr Met Leu Pro Arg Ala Ser Ile Asp Gly Phe 
    290                 295                 300                 


Ala Glu Ala Gly Ala Glu Arg Gln Glu Tyr Leu Leu Phe Val Arg Tyr 
305                 310                 315                 320 


Ala Ala Leu Leu Val Asp Pro Phe Ser Pro Ala Asp Glu Gln Gly Arg 
                325                 330                 335     


His Phe Asp Phe Ser Ala Val Pro Phe Arg Arg Val Ser Arg Asp Glu 
            340                 345                 350         


Asp Gly Leu Trp Arg Leu Pro Arg Val Pro Leu Glu Asp Tyr Arg Lys 
        355                 360                 365             


Leu Ala Leu Ile Val Ala Leu Ala Ala His Phe Asp Leu Ala Met Pro 
    370                 375                 380                 


Gln Ala Arg Ser Leu Leu Ala Ser Tyr Glu Asn Ala Val Ser Arg Phe 
385                 390                 395                 400 


Ile Asp Cys Gln Gly Ala Ser Gln Cys His Pro Ser Leu Tyr Pro Ile 
                405                 410                 415     


Asp Ser Arg Pro Ala Ala Asp Ala Ile Tyr Arg Gln Trp Cys Ser Thr 
            420                 425                 430         


Cys 
    


<210>  6
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PA-ND-IF-F

<400>  6
actagtctag aagcttagcc tgaccctgaa ctactg                                 36


<210>  7
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PA-ND-IF-R

<400>  7
agaactagtg gatcctgaag gtgaaggacg ccag                                   34


<210>  8
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SA-END-IF-F

<400>  8
actagtctag aagcttacca actgcataag agcctc                                 36


<210>  9
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SA-END-IF-R

<400>  9
agaactagtg gatccgatgc aagtaacatt gcactc                                 36


<210>  10
<211>  1059
<212>  DNA
<213>  Rhodopseudomonas palustris

<400>  10
atgaccgatc tcgttctgcc cgcttcttcc gatggattcc gcttccgcct ggtcgcggtg       60

ctcggcggcc tggtgctcgc caatatcgcc gcctgggcct gggcgctgac cgcgttcgcg      120

ggcgagccgg tgctgatcgg caccgcggtt ctggcctata gccttggcct gcgccacgcg      180

ctcgacgccg accatatcgc ggcgatcgac aacgtcaccc gcaagctgat gcaggagggc      240

aagcggccgg tcgcggtcgg gctgttcttc gcgctcggcc attccaccgt cgtgctggtg      300

gcgtcgctgg cgatcgcagt cgcggccaat tcgctgaccg aacggttctc gggctatcgc      360

gagatcggcg gggtgatcgg cacctcggcg tcggcgctgt ttctgttcgc gatcgccatc      420

gccaatctgt cggtgctgag cggcgtgtat cgggcgttca agaaggccaa ggccggcgag      480

gtcgtgcacg acgaagacat caacgcgctg ctgcagcagc gcggctggct ggcgcggctg      540

ttccggccgc tgttccgctt cgtctcgaag agctggcagt tgtttccgat cgggctattg      600

ttcgcgctcg gcttcgagac cgccagcgaa atcagcctgt tcggactggc cgccaacgcc      660

tccggcagca tttcgcactg gacgatcctg gtgttcccgg cgctgtttgc cgccggtatg      720

accctggtcg acaccctcga cggcgtgctg atgctcggcg cctatggctg ggcgtatcgc      780

aatccgatcc gcaagctgta ctacaacatg acgatcacga cggtgtcggt gctggtggcg      840

ctgctgatcg gcggcatcga gacgctcgga ctgctcgccg ggcgcttcca tctcgaaggc      900

gcgttctggg agtggatcga agaactcaat tccaatttcg gcgcgctcgg ttacggcatc      960

gtggcgctgt tcgtggcgag ctggatcgtc tccaccatca tctatcgcct caacggctat     1020

caccggctcg accaggcagc cgcggccgtg cagaaatga                            1059


<210>  11
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mam-F

<400>  11
ctcgaggagc tcaagctttt ccaatgacca ccaccac                                37


<210>  12
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mam-R

<400>  12
gtcgacggat ccactagtct gatctccggc                                        30


<210>  13
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RpNxia-F

<400>  13
actagtggat ccatgaccga tctcgttc                                          28


<210>  14
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RpNxia-R

<400>  14
ggtaccgaat tctcatttct gcacggcc                                          28


