                               SEQUENCE LISTING

<110> TUFTS UNIVERSITY
      TUFTS MEDICAL CENTER
 
<120> NOVEL POLYPEPTIDES WITH IMPROVED PROTEOLYTIC STABILITY, AND 
      METHODS OF PREPARING AND USING SAME

<130> 167774.011001 PCT1

<140> PCT/US2016/059541
<141> 2016-10-28

<150> 62/247,493
<151> 2015-10-28

<160> 33    

<170> PatentIn version 3.5

<210> 1
<211> 30
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (30)..(30)
<223> unmodified or amidation

<400> 1
His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg 
            20                  25                  30  


<210> 2
<211> 39
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (39)..(39)
<223> unmodified or amidation

<400> 2
His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu 
1               5                   10                  15      


Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210> 3
<211> 31
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210> 4
<211> 31
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (2)..(2)
<223> 2-Aminoisobutyric acid 

<400> 4
His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210> 5
<211> 30
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (2)..(2)
<223> 2-Aminoisobutyric acid 

<220>
<221> MOD_RES
<222> (29)..(29)
<223> 2-Aminoisobutyric acid 

<220>
<221> MOD_RES
<222> (30)..(30)
<223> unmodified or amidation

<400> 5
His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Xaa Arg 
            20                  25                  30  


<210> 6
<211> 44
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (44)..(44)
<223> unmodified or amidation

<400> 6
His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu 
1               5                   10                  15      


Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Ser Lys Lys Lys Lys Lys Lys 
        35                  40                  


<210> 7
<211> 39
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (2)..(2)
<223> 2-Aminoisobutyric acid 

<220>
<221> MOD_RES
<222> (39)..(39)
<223> unmodified or amidation

<400> 7
His Xaa Gln Gly Thr Phe Thr Ser Asp Lys Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Arg Ala Ala Gln Asp Phe Val Gln Trp Leu Leu Asp Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210> 8
<211> 28
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 8
His Ser Asp Ala Val Phe Thr Asp Asn Tyr Thr Arg Leu Arg Lys Gln 
1               5                   10                  15      


Met Ala Val Lys Lys Tyr Leu Asn Ser Ile Leu Asn 
            20                  25              


<210> 9
<211> 38
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 9
His Ser Asp Gly Ile Phe Thr Asp Ser Tyr Ser Arg Tyr Arg Lys Gln 
1               5                   10                  15      


Met Ala Val Lys Lys Tyr Leu Ala Ala Val Leu Gly Lys Arg Tyr Lys 
            20                  25                  30          


Gln Arg Val Lys Asn Lys 
        35              


<210> 10
<211> 42
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 10
Tyr Ala Glu Gly Thr Phe Ile Ser Asp Tyr Ser Ile Ala Met Asp Lys 
1               5                   10                  15      


Ile His Gln Gln Asp Phe Val Asn Trp Leu Leu Ala Gln Lys Gly Lys 
            20                  25                  30          


Lys Asn Asp Trp Lys His Asn Ile Thr Gln 
        35                  40          


<210> 11
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 11
Tyr Gly Gly Phe Met 
1               5   


<210> 12
<211> 36
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 12
Tyr Pro Ser Lys Pro Asp Asn Pro Gly Glu Asp Ala Pro Ala Glu Asp 
1               5                   10                  15      


Met Ala Arg Tyr Tyr Ser Ala Leu Arg His Tyr Ile Asn Leu Ile Thr 
            20                  25                  30          


Arg Gln Arg Tyr 
        35      


<210> 13
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 13
Arg Pro Lys Pro Gln Gln Phe Phe Gly Leu Met 
1               5                   10      


<210> 14
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 14
Tyr Pro Trp Gly 
1               


<210> 15
<211> 29
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 15
His Ser Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Ser 
1               5                   10                  15      


Arg Arg Ala Gln Asp Phe Val Gln Trp Leu Met Asn Thr 
            20                  25                  


<210> 16
<211> 235
<212> PRT
<213> Homo sapiens

<400> 16
Asp Glu Ser Ala Cys Leu Gln Ala Ala Glu Glu Met Pro Asn Thr Thr 
1               5                   10                  15      


Leu Gly Cys Pro Ala Thr Trp Asp Gly Leu Leu Cys Trp Pro Thr Ala 
            20                  25                  30          


Gly Ser Gly Glu Trp Val Thr Leu Pro Cys Pro Asp Phe Phe Ser His 
        35                  40                  45              


Phe Ser Ser Glu Ser Gly Ala Val Lys Arg Asp Cys Thr Ile Thr Gly 
    50                  55                  60                  


Trp Ser Glu Pro Phe Pro Pro Tyr Pro Val Ala Cys Pro Val Pro Leu 
65                  70                  75                  80  


Glu Leu Leu Ala Glu Glu Glu Ser Tyr Phe Ser Thr Val Lys Ile Ile 
                85                  90                  95      


Tyr Thr Val Gly His Ser Ile Ser Ile Val Ala Leu Phe Val Ala Ile 
            100                 105                 110         


Thr Ile Leu Val Ala Leu Arg Arg Leu His Cys Pro Arg Asn Tyr Val 
        115                 120                 125             


His Thr Gln Leu Phe Thr Thr Phe Ile Leu Lys Ala Gly Ala Val Phe 
    130                 135                 140                 


Leu Lys Asp Ala Ala Leu Phe His Ser Asp Asp Thr Asp His Cys Ser 
145                 150                 155                 160 


Phe Ser Thr Val Leu Cys Lys Val Ser Val Ala Ala Ser His Phe Ala 
                165                 170                 175     


Thr Met Thr Asn Phe Ser Trp Leu Leu Ala Glu Ala Val Tyr Leu Asn 
            180                 185                 190         


Cys Leu Leu Ala Ser Thr Ser Pro Ser Ser Arg Arg Ala Phe Trp Trp 
        195                 200                 205             


Leu Val Leu Ala Gly Trp Gly Leu Pro Val Leu Phe Thr Gly Thr Trp 
    210                 215                 220                 


Val Ser Cys Lys Leu Ala Phe Glu Asp Ile Ala 
225                 230                 235 


<210> 17
<211> 29
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (29)..(29)
<223> unmodified or amidation

<400> 17
Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Met Ser Arg 
            20                  25                  


<210> 18
<211> 44
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (44)..(44)
<223> unmodified or amidation

<400> 18
Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Met Ser Arg Gln Gln Gly 
            20                  25                  30          


Glu Ser Asn Gln Glu Arg Gly Ala Arg Ala Arg Leu 
        35                  40                  


<210> 19
<211> 31
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 19
His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Gly 
            20                  25                  30      


<210> 20
<211> 33
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 20
His Ala Asp Gly Ser Phe Ser Asp Glu Met Asn Thr Ile Leu Asp Asn 
1               5                   10                  15      


Leu Ala Ala Arg Asp Phe Ile Asn Trp Leu Ile Gln Thr Lys Ile Thr 
            20                  25                  30          


Asp 
    


<210> 21
<211> 13
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 21
Glu Leu Tyr Glu Asn Lys Pro Arg Arg Pro Tyr Ile Leu 
1               5                   10              


<210> 22
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 22
Glu His Trp Ser Tyr Gly Leu Arg Pro Gly 
1               5                   10  


<210> 23
<211> 40
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 23
Tyr Ala Glu Gly Thr Phe Thr Ser Asp Tyr Ser Ile Tyr Leu Asp Lys 
1               5                   10                  15      


Gln Ala Ala Ala Glu Phe Val Asn Trp Leu Leu Ala Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser Lys 
        35                  40  


<210> 24
<211> 39
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 24
His Ala Gln Gly Thr Phe Thr Ser Asp Lys Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Arg Ala Ala Gln Asp Phe Val Gln Trp Leu Leu Asp Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210> 25
<211> 37
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 25
His Ser Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Ser 
1               5                   10                  15      


Arg Arg Ala Gln Asp Phe Val Gln Trp Leu Met Asn Thr Lys Arg Asn 
            20                  25                  30          


Arg Asn Asn Ile Ala 
        35          


<210> 26
<211> 33
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 26
His Gly Asp Gly Ser Phe Ser Asp Glu Met Asn Thr Ile Leu Asp Asn 
1               5                   10                  15      


Leu Ala Ala Arg Asp Phe Ile Asn Trp Leu Ile Gln Thr Lys Ile Thr 
            20                  25                  30          


Asp 
    


<210> 27
<211> 33
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 27
His Ala Glu Gly Ser Phe Ser Asp Glu Met Asn Thr Ile Leu Asp Asn 
1               5                   10                  15      


Lys Ala Ala Arg Asp Phe Ile Asn Trp Leu Ile Gln Thr Lys Ile Thr 
            20                  25                  30          


Asp 
    


<210> 28
<211> 33
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 28
His Ala Asp Gly Ser Phe Ser Asp Glu Met Asn Thr Ile Leu Asp Asn 
1               5                   10                  15      


Leu Ala Ala Arg Asp Phe Ile Lys Trp Leu Ile Gln Thr Lys Ile Thr 
            20                  25                  30          


Asp 
    


<210> 29
<211> 29
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 29
Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln 
1               5                   10                  15      


Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg 
            20                  25                  


<210> 30
<211> 41
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 30
Ala Glu Gly Thr Phe Ile Ser Asp Tyr Ser Ile Ala Met Asp Lys Ile 
1               5                   10                  15      


His Gln Gln Asp Phe Val Asn Trp Leu Leu Ala Gln Lys Gly Lys Lys 
            20                  25                  30          


Asn Asp Trp Lys His Asn Ile Thr Gln 
        35                  40      


<210> 31
<211> 28
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 31
Ser Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Ser Arg 
1               5                   10                  15      


Arg Ala Gln Asp Phe Val Gln Trp Leu Met Asn Thr 
            20                  25              


<210> 32
<211> 38
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 32
Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu Glu 
1               5                   10                  15      


Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser Ser 
            20                  25                  30          


Gly Ala Pro Pro Pro Ser 
        35              


<210> 33
<211> 38
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 33
Ala Gln Gly Thr Phe Thr Ser Asp Lys Ser Lys Tyr Leu Asp Glu Arg 
1               5                   10                  15      


Ala Ala Gln Asp Phe Val Gln Trp Leu Leu Asp Gly Gly Pro Ser Ser 
            20                  25                  30          


Gly Ala Pro Pro Pro Ser 
        35              


