			Sequence Listing

<110>	NBE therapeutics

<120>	anti-Mesothelin antibodiess

<130>	ND40471

<160>	22

<170>	Excel Makro
	
	

<210>	1
<212>	PRT
<213>	artificial

<220>	VH-MN   CDR1 HC
<211>	107

<400>	1
Gly Tyr Thr Phe Thr Ser Tyr Trp          	
1               5                   	               
	

<210>	2
<212>	PRT
<213>	artificial

<220>	VH-MN   CDR2 HC
<211>	8

<400>	2
Ile His Pro Asn Ser Asp Asn Thr          	
1               5                   	               
	

<210>	3
<212>	PRT
<213>	artificial

<220>	VH-MN   CDR3 HC
<211>	13

<400>	3
Ala Ile Ile Ile Thr Pro Val Val Pro Lys Phe Asp Tyr          	
1               5                   10	               
	

<210>	4
<212>	PRT
<213>	artificial

<220>	VH-MN   CDR1 LC
<211>	6

<400>	4
His Asp Val Gly Thr Ser          	
1               5                   	               
	

<210>	5
<212>	PRT
<213>	artificial

<220>	VH-MN   CDR2 LC
<211>	3

<400>	5
Trp Ala Ser          	
1                                  	               
	

<210>	6
<212>	PRT
<213>	artificial

<220>	VH-MN   CDR3 LC
<211>	9

<400>	6
Gln Gln Tyr Ser Ser Tyr Pro Leu Thr          	
1               5                   	               
	

<210>	7
<212>	PRT
<213>	artificial

<220>	VH-MN (parental mouse)  VR HC
<211>	120

<400>	7
Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10	               15

Ser Met Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30     

Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45 

Gly Met Ile His Pro Asn Ser Asp Asn Thr Ile Tyr Tyr Glu Lys Phe 
    50                  55                  60      

Lys Ser Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95    

Ala Ile Ile Ile Thr Pro Val Val Pro Lys Phe Asp Tyr Trp Gly Gln 
            100                 105                 110  

Gly Thr Thr Leu Thr Val Ser Ser          	
        115
	

<210>	8
<212>	PRT
<213>	artificial

<220>	VH-MN (parental mouse)  VR LC
<211>	107

<400>	8
Asp Ile Val Met Thr Gln Ser His Gln Phe Met Ser Thr Ser Val Gly 
1               5                   10	               15

Asp Arg Val Ser Val Thr Cys Lys Ala Ser His Asp Val Gly Thr Ser 
            20                  25                  30     

Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45 

Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60      

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Asn Val Gln Ser 
65                  70                  75                  80

Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Ser Ser Tyr Pro Leu 
                85                  90                  95    

Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys          	
            100                 105                   
	

<210>	9
<212>	PRT
<213>	artificial

<220>	VH-MN clone 3-1 (humanized)  VR HC
<211>	120

<400>	9
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10	               15

Ser Met Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30     

Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45 

Gly Ala Ile His Pro Asn Ser Asp Asn Thr Ile Tyr Tyr Gln Lys Phe 
    50                  55                  60      

Lys Ser Arg Val Thr Leu Thr Val Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95    

Ala Ile Ile Ile Thr Pro Val Val Pro Lys Phe Asp Tyr Trp Gly Gln 
            100                 105                 110  

Gly Thr Thr Val Thr Val Ser Ser          	
        115
	

<210>	10
<212>	PRT
<213>	artificial

<220>	VH-MN clone 3-1 (humanized)  VR LC
<211>	107

<400>	10
Asp Ile Gln Met Thr Gln Ser Pro Ser Phe Leu Ser Ala Ser Val Gly 
1               5                   10	               15

Asp Arg Val Thr Ile Thr Cys Arg Ala Ser His Asp Val Gly Thr Ser 
            20                  25                  30     

Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Lys Leu Leu Ile 
        35                  40                  45 

Tyr Trp Ala Ser Thr Leu Gln Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60      

Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80

Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Ser Tyr Pro Leu 
                85                  90                  95    

Thr Phe Gly Pro Gly Thr Thr Val Asp Met Lys          	
            100                 105                   
	

<210>	11
<212>	PRT
<213>	artificial

<220>	VH-MN clone 5-2 (humanized)  VR HC
<211>	120

<400>	11
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10	               15

Ser Leu Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30     

Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45 

Gly Ala Ile His Pro Asn Ser Asp Asn Thr Ile Tyr Tyr Gln Lys Phe 
    50                  55                  60      

Lys Ser Arg Phe Thr Ile Thr Val Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95    

Ala Ile Ile Ile Thr Pro Val Val Pro Lys Phe Asp Tyr Trp Gly Gln 
            100                 105                 110  

Gly Thr Thr Val Thr Val Ser Ser          	
        115
	

<210>	12
<212>	PRT
<213>	artificial

<220>	VH-MN clone 5-2 (humanized)  VR LC
<211>	107

<400>	12
Asp Ile Val Met Thr Gln Ser Ser Ser Phe Met Ser Ala Ser Val Gly 
1               5                   10	               15

Asp Arg Val Ser Ile Thr Cys Lys Ala Ser His Asp Val Gly Thr Ser 
            20                  25                  30     

Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45 

Tyr Trp Ala Ser Thr Arg Gln Thr Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60      

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ser 
65                  70                  75                  80

Glu Asp Val Ala Thr Tyr Phe Cys Gln Gln Tyr Ser Ser Tyr Pro Leu 
                85                  90                  95    

Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys          	
            100                 105                   
	

<210>	13
<212>	PRT
<213>	artificial

<220>	VH-MN clone 5-3 (humanized)  VR HC
<211>	120

<400>	13
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10	               15

Ser Leu Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30     

Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45 

Gly Ala Ile His Pro Asn Ser Asp Asn Thr Ile Tyr Tyr Gln Lys Phe 
    50                  55                  60      

Lys Ser Arg Phe Thr Ile Thr Val Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95    

Ala Ile Ile Ile Thr Pro Val Val Pro Lys Phe Asp Tyr Trp Gly Gln 
            100                 105                 110  

Gly Thr Thr Val Thr Val Ser Ser          	
        115
	

<210>	14
<212>	PRT
<213>	artificial

<220>	VH-MN clone 5-3 (humanized)  VR LC
<211>	107

<400>	14
Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10	               15

Glu Arg Ala Thr Ile Thr Cys Lys Ser Ser His Asp Val Gly Thr Ser 
            20                  25                  30     

Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile 
        35                  40                  45 

Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60      

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala 
65                  70                  75                  80

Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Tyr Ser Ser Tyr Pro Leu 
                85                  90                  95    

Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys          	
            100                 105                 110  
	

<210>	15
<212>	DNA/RNA
<213>	artificial

<220>	forward primer univ-Not1-SP-F
<211>	27

<400>	15
gaggaggcgg ccgccatgaa ctttggg       	
	

<210>	16
<212>	DNA/RNA
<213>	artificial

<220>	reverse primers huCG1-B
<211>	21

<400>	16
aagaccgatg ggcccttggt g       	
	

<210>	17
<212>	DNA/RNA
<213>	artificial

<220>	IgHV and huCK-B 
<211>	21

<400>	17
gaagacagat ggtgcagcca c       	
	

<210>	18
<212>	DNA/RNA
<213>	artificial

<220>	primer pPBseq13 
<211>	20

<400>	18
ggccagcttg gcacttgatg        	
	

<210>	19
<212>	DNA/RNA
<213>	artificial

<220>	EF1aNotI_F 
<211>	20

<400>	19
ccatttcagg tgtcgtgagc        	
	

<210>	20
<212>	DNA/RNA
<213>	artificial

<220>	CG-revseq-1
<211>	20

<400>	20
gttcggggaa gtagtccttg        	
	

<210>	21
<212>	DNA/RNA
<213>	artificial

<220>	Intron-rev-1
<211>	28

<400>	21
gtggatgtca gtaagaccaa taggtgcc       	
	

<210>	22
<212>	DNA/RNA
<213>	artificial

<220>	CMVseq2
<211>	21

<400>	22
gcagtgtagt ctgagcagta c  
       	


