                         SEQUENCE LISTING

<110>  Monash University
 
<120>  Methods and compositions for improving glucose metabolism

<130>  50146571AZD

<150>  AU2015904460
<151>  2015-10-30

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  435
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Leu Pro Ala Arg Cys Ala Arg Leu Leu Thr Pro His Leu Leu Leu 
1               5                   10                  15      


Val Leu Val Gln Leu Ser Pro Ala Arg Gly His Arg Thr Thr Gly Pro 
            20                  25                  30          


Arg Phe Leu Ile Ser Asp Arg Asp Pro Gln Cys Asn Leu His Cys Ser 
        35                  40                  45              


Arg Thr Gln Pro Lys Pro Ile Cys Ala Ser Asp Gly Arg Ser Tyr Glu 
    50                  55                  60                  


Ser Met Cys Glu Tyr Gln Arg Ala Lys Cys Arg Asp Pro Thr Leu Gly 
65                  70                  75                  80  


Val Val His Arg Gly Arg Cys Lys Asp Ala Gly Gln Ser Lys Cys Arg 
                85                  90                  95      


Leu Glu Arg Ala Gln Ala Leu Glu Gln Ala Lys Lys Pro Gln Glu Ala 
            100                 105                 110         


Val Phe Val Pro Glu Cys Gly Glu Asp Gly Ser Phe Thr Gln Val Gln 
        115                 120                 125             


Cys His Thr Tyr Thr Gly Tyr Cys Trp Cys Val Thr Pro Asp Gly Lys 
    130                 135                 140                 


Pro Ile Ser Gly Ser Ser Val Gln Asn Lys Thr Pro Val Cys Ser Gly 
145                 150                 155                 160 


Ser Val Thr Asp Lys Pro Leu Ser Gln Gly Asn Ser Gly Arg Lys Asp 
                165                 170                 175     


Asp Gly Ser Lys Pro Thr Pro Thr Met Glu Thr Gln Pro Val Phe Asp 
            180                 185                 190         


Gly Asp Glu Ile Thr Ala Pro Thr Leu Trp Ile Lys His Leu Val Ile 
        195                 200                 205             


Lys Asp Ser Lys Leu Asn Asn Thr Asn Ile Arg Asn Ser Glu Lys Val 
    210                 215                 220                 


Tyr Ser Cys Asp Gln Glu Arg Gln Ser Ala Leu Glu Glu Ala Gln Gln 
225                 230                 235                 240 


Asn Pro Arg Glu Gly Ile Val Ile Pro Glu Cys Ala Pro Gly Gly Leu 
                245                 250                 255     


Tyr Lys Pro Val Gln Cys His Gln Ser Thr Gly Tyr Cys Trp Cys Val 
            260                 265                 270         


Leu Val Asp Thr Gly Arg Pro Leu Pro Gly Thr Ser Thr Arg Tyr Val 
        275                 280                 285             


Met Pro Ser Cys Glu Ser Asp Ala Arg Ala Lys Thr Thr Glu Ala Asp 
    290                 295                 300                 


Asp Pro Phe Lys Asp Arg Glu Leu Pro Gly Cys Pro Glu Gly Lys Lys 
305                 310                 315                 320 


Met Glu Phe Ile Thr Ser Leu Leu Asp Ala Leu Thr Thr Asp Met Val 
                325                 330                 335     


Gln Ala Ile Asn Ser Ala Ala Pro Thr Gly Gly Gly Arg Phe Ser Glu 
            340                 345                 350         


Pro Asp Pro Ser His Thr Leu Glu Glu Arg Val Val His Trp Tyr Phe 
        355                 360                 365             


Ser Gln Leu Asp Ser Asn Ser Ser Asn Asp Ile Asn Lys Arg Glu Met 
    370                 375                 380                 


Lys Pro Phe Lys Arg Tyr Val Lys Lys Lys Ala Lys Pro Lys Lys Cys 
385                 390                 395                 400 


Ala Arg Arg Phe Thr Asp Tyr Cys Asp Leu Asn Lys Asp Lys Val Ile 
                405                 410                 415     


Ser Leu Pro Glu Leu Lys Gly Cys Leu Gly Val Ser Lys Glu Val Gly 
            420                 425                 430         


Arg Leu Val 
        435 


<210>  2
<211>  1308
<212>  DNA
<213>  Homo sapiens

<400>  2
atgctgcccg cgcgctgcgc ccgcctgctc acgccccact tgctgctggt gttggtgcag       60

ctgtcccctg ctcgcggcca ccgcaccaca ggccccaggt ttctaataag tgaccgtgac      120

ccacaatgca acctccactg ctccaggact caacccaaac ccatctgtgc ctctgatggc      180

aggtcctacg agtccatgtg tgagtaccag cgagccaagt gccgagaccc gaccctgggc      240

gtggtgcatc gaggtagatg caaagatgct ggccagagca agtgtcgcct ggagcgggct      300

caagccctgg agcaagccaa gaagcctcag gaagctgtgt ttgtcccaga gtgtggcgag      360

gatggctcct ttacccaggt gcagtgccat acttacactg ggtactgctg gtgtgtcacc      420

ccggatggga agcccatcag tggctcttct gtgcagaata aaactcctgt atgttcaggt      480

tcagtcaccg acaagccctt gagccagggt aactcaggaa ggaaagatga cgggtctaag      540

ccgacaccca cgatggagac ccagccggtg ttcgatggag atgaaatcac agccccaact      600

ctatggatta aacacttggt gatcaaggac tccaaactga acaacaccaa cataagaaat      660

tcagagaaag tctattcgtg tgaccaggag aggcagagtg ctctggaaga ggcccagcag      720

aatccccgtg agggtattgt catccctgaa tgtgcccctg ggggactcta taagccagtg      780

caatgccacc agtccactgg ctactgctgg tgtgtgctgg tggacacagg gcgcccgctg      840

cctgggacct ccacacgcta cgtgatgccc agttgtgaga gcgacgccag ggccaagact      900

acagaggcgg atgacccctt caaggacagg gagctaccag gctgtccaga agggaagaaa      960

atggagttta tcaccagcct actggatgct ctcaccactg acatggttca ggccattaac     1020

tcagcagcgc ccactggagg tgggaggttc tcagagccag accccagcca caccctggag     1080

gagcgggtag tgcactggta tttcagccag ctggacagca atagcagcaa cgacattaac     1140

aagcgggaga tgaagccctt caagcgctac gtgaagaaga aagccaagcc caagaaatgt     1200

gcccggcgtt tcaccgacta ctgtgacctg aacaaagaca aggtcatttc actgcctgag     1260

ctgaagggct gcctgggtgt tagcaaagaa gtaggacgcc tcgtctaa                  1308


<210>  3
<211>  670
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protein sequence of SMOC1-Fc fusion protein

<400>  3

Met Leu Pro Ala Arg Cys Ala Arg Leu Leu Thr Pro His Leu Leu Leu 
1               5                   10                  15      


Val Leu Val Gln Leu Ser Pro Ala Arg Gly His Arg Thr Thr Gly Pro 
            20                  25                  30          


Arg Phe Leu Ile Ser Asp Arg Asp Pro Gln Cys Asn Leu His Cys Ser 
        35                  40                  45              


Arg Thr Gln Pro Lys Pro Ile Cys Ala Ser Asp Gly Arg Ser Tyr Glu 
    50                  55                  60                  


Ser Met Cys Glu Tyr Gln Arg Ala Lys Cys Arg Asp Pro Thr Leu Gly 
65                  70                  75                  80  


Val Val His Arg Gly Arg Cys Lys Asp Ala Gly Gln Ser Lys Cys Arg 
                85                  90                  95      


Leu Glu Arg Ala Gln Ala Leu Glu Gln Ala Lys Lys Pro Gln Glu Ala 
            100                 105                 110         


Val Phe Val Pro Glu Cys Gly Glu Asp Gly Ser Phe Thr Gln Val Gln 
        115                 120                 125             


Cys His Thr Tyr Thr Gly Tyr Cys Trp Cys Val Thr Pro Asp Gly Lys 
    130                 135                 140                 


Pro Ile Ser Gly Ser Ser Val Gln Asn Lys Thr Pro Val Cys Ser Gly 
145                 150                 155                 160 


Ser Val Thr Asp Lys Pro Leu Ser Gln Gly Asn Ser Gly Arg Lys Asp 
                165                 170                 175     


Asp Gly Ser Lys Pro Thr Pro Thr Met Glu Thr Gln Pro Val Phe Asp 
            180                 185                 190         


Gly Asp Glu Ile Thr Ala Pro Thr Leu Trp Ile Lys His Leu Val Ile 
        195                 200                 205             


Lys Asp Ser Lys Leu Asn Asn Thr Asn Ile Arg Asn Ser Glu Lys Val 
    210                 215                 220                 


Tyr Ser Cys Asp Gln Glu Arg Gln Ser Ala Leu Glu Glu Ala Gln Gln 
225                 230                 235                 240 


Asn Pro Arg Glu Gly Ile Val Ile Pro Glu Cys Ala Pro Gly Gly Leu 
                245                 250                 255     


Tyr Lys Pro Val Gln Cys His Gln Ser Thr Gly Tyr Cys Trp Cys Val 
            260                 265                 270         


Leu Val Asp Thr Gly Arg Pro Leu Pro Gly Thr Ser Thr Arg Tyr Val 
        275                 280                 285             


Met Pro Ser Cys Glu Ser Asp Ala Arg Ala Lys Thr Thr Glu Ala Asp 
    290                 295                 300                 


Asp Pro Phe Lys Asp Arg Glu Leu Pro Gly Cys Pro Glu Gly Lys Lys 
305                 310                 315                 320 


Met Glu Phe Ile Thr Ser Leu Leu Asp Ala Leu Thr Thr Asp Met Val 
                325                 330                 335     


Gln Ala Ile Asn Ser Ala Ala Pro Thr Gly Gly Gly Arg Phe Ser Glu 
            340                 345                 350         


Pro Asp Pro Ser His Thr Leu Glu Glu Arg Val Val His Trp Tyr Phe 
        355                 360                 365             


Ser Gln Leu Asp Ser Asn Ser Ser Asn Asp Ile Asn Lys Arg Glu Met 
    370                 375                 380                 


Lys Pro Phe Lys Arg Tyr Val Lys Lys Lys Ala Lys Pro Lys Lys Cys 
385                 390                 395                 400 


Ala Arg Arg Phe Thr Asp Tyr Cys Asp Leu Asn Lys Asp Lys Val Ile 
                405                 410                 415     


Ser Leu Pro Glu Leu Lys Gly Cys Leu Gly Val Ser Lys Glu Val Gly 
            420                 425                 430         


Arg Leu Val Ser His His His His His His Ala Ser Asp Lys Thr His 
        435                 440                 445             


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
    450                 455                 460                 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
465                 470                 475                 480 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
                485                 490                 495     


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
            500                 505                 510         


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
        515                 520                 525             


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
    530                 535                 540                 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
545                 550                 555                 560 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
                565                 570                 575     


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
            580                 585                 590         


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
        595                 600                 605             


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
    610                 615                 620                 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
625                 630                 635                 640 


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
                645                 650                 655     


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
            660                 665                 670 


<210>  4
<211>  2149
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid sequence encoding SMOC1-Fc fusion protein

<400>  4
atgggctgga gcctgatcct cctgttcctc gtcgctgtgg ctacaggtaa ggggctcaca       60

gtagcaggct tgaggtctgg acatatatat gggtgacaat gacatccact ttgcctttct      120

ctccacaggt ggcgcgcatg ctgcccgcgc gctgcgcccg cctgctcacg ccccacttgc      180

tgctggtgtt ggtgcagctg tcccctgctc gcggccaccg caccacaggc cccaggtttc      240

taataagtga ccgtgaccca caatgcaacc tccactgctc caggactcaa cccaaaccca      300

tctgtgcctc tgatggcagg tcctacgagt ccatgtgtga gtaccagcga gccaagtgcc      360

gagacccgac cctgggcgtg gtgcatcgag gtagatgcaa agatgctggc cagagcaagt      420

gtcgcctgga gcgggctcaa gccctggagc aagccaagaa gcctcaggaa gctgtgtttg      480

tcccagagtg tggcgaggat ggctccttta cccaggtgca gtgccatact tacactgggt      540

actgctggtg tgtcaccccg gatgggaagc ccatcagtgg ctcttctgtg cagaataaaa      600

ctcctgtatg ttcaggttca gtcaccgaca agcccttgag ccagggtaac tcaggaagga      660

aagatgacgg gtctaagccg acacccacga tggagaccca gccggtgttc gatggagatg      720

aaatcacagc cccaactcta tggattaaac acttggtgat caaggactcc aaactgaaca      780

acaccaacat aagaaattca gagaaagtct attcgtgtga ccaggagagg cagagtgctc      840

tggaagaggc ccagcagaat ccccgtgagg gtattgtcat ccctgaatgt gcccctgggg      900

gactctataa gccagtgcaa tgccaccagt ccactggcta ctgctggtgt gtgctggtgg      960

acacagggcg cccgctgcct gggacctcca cacgctacgt gatgcccagt tgtgagagcg     1020

acgccagggc caagactaca gaggcggatg accccttcaa ggacagggag ctaccaggct     1080

gtccagaagg gaagaaaatg gagtttatca ccagcctact ggatgctctc accactgaca     1140

tggttcaggc cattaactca gcagcgccca ctggaggtgg gaggttctca gagccagacc     1200

ccagccacac cctggaggag cgggtagtgc actggtattt cagccagctg gacagcaata     1260

gcagcaacga cattaacaag cgggagatga agcccttcaa gcgctacgtg aagaagaaag     1320

ccaagcccaa gaaatgtgcc cggcgtttca ccgactactg tgacctgaac aaagacaagg     1380

tcatttcact gcctgagctg aagggctgcc tgggtgttag caaagaagta ggacgcctcg     1440

tctcacatca tcaccatcac cacgctagcg acaaaactca cacatgccca ccgtgcccag     1500

cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc     1560

tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc     1620

ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc aagacaaagc     1680

cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc     1740

aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc ctcccagccc     1800

ccatcgagaa aaccatctcc aaagccaaag ggcagccccg agaaccacag gtgtacaccc     1860

tgcccccatc ccgggaggag atgaccaaga accaggtcag cctgacctgc ctggtcaaag     1920

gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg gagaacaact     1980

acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctat agcaagctca     2040

ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg atgcatgagg     2100

ctctgcacaa ccactacacg cagaagagcc tctccctgtc cccgggtaa                 2149


