                         SEQUENCE LISTING

<110>  Autolus Ltd
 
<120>  RECEPTOR

<130>  P108498PCT

<150>  GB 1518816.2
<151>  2015-10-23

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endodomain derived from human common gamma chain

<400>  1

Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys Asn Leu Glu Asp Leu 
1               5                   10                  15      


Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp Ser Gly Val Ser Lys 
            20                  25                  30          


Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser Glu Arg Leu Cys Leu 
        35                  40                  45              


Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu Gly Glu Gly Pro Gly 
    50                  55                  60                  


Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp Ala Pro Pro Cys Tyr 
65                  70                  75                  80  


Thr Leu Lys Pro Glu Thr 
                85      


<210>  2
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endodomain derived from human IL-2Rbeta

<400>  2

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
145                 150                 155                 160 


Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro 
                165                 170                 175     


Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro 
            180                 185                 190         


Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly 
        195                 200                 205             


Pro Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro 
    210                 215                 220                 


Glu Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro 
225                 230                 235                 240 


Arg Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu 
                245                 250                 255     


Phe Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu 
            260                 265                 270         


Ser Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
        275                 280                 285     


<210>  3
<211>  195
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endodomain derived from human IL-7Ralpha

<400>  3

Lys Lys Arg Ile Lys Pro Ile Val Trp Pro Ser Leu Pro Asp His Lys 
1               5                   10                  15      


Lys Thr Leu Glu His Leu Cys Lys Lys Pro Arg Lys Asn Leu Asn Val 
            20                  25                  30          


Ser Phe Asn Pro Glu Ser Phe Leu Asp Cys Gln Ile His Arg Val Asp 
        35                  40                  45              


Asp Ile Gln Ala Arg Asp Glu Val Glu Gly Phe Leu Gln Asp Thr Phe 
    50                  55                  60                  


Pro Gln Gln Leu Glu Glu Ser Glu Lys Gln Arg Leu Gly Gly Asp Val 
65                  70                  75                  80  


Gln Ser Pro Asn Cys Pro Ser Glu Asp Val Val Ile Thr Pro Glu Ser 
                85                  90                  95      


Phe Gly Arg Asp Ser Ser Leu Thr Cys Leu Ala Gly Asn Val Ser Ala 
            100                 105                 110         


Cys Asp Ala Pro Ile Leu Ser Ser Ser Arg Ser Leu Asp Cys Arg Glu 
        115                 120                 125             


Ser Gly Lys Asn Gly Pro His Val Tyr Gln Asp Leu Leu Leu Ser Leu 
    130                 135                 140                 


Gly Thr Thr Asn Ser Thr Leu Pro Pro Pro Phe Ser Leu Gln Ser Gly 
145                 150                 155                 160 


Ile Leu Thr Leu Asn Pro Val Ala Gln Gly Gln Pro Ile Leu Thr Ser 
                165                 170                 175     


Leu Gly Ser Asn Gln Glu Glu Ala Tyr Val Thr Met Ser Ser Phe Tyr 
            180                 185                 190         


Gln Asn Gln 
        195 


<210>  4
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge-CH2CH3 of human IgG1

<400>  4

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp 
225                 230                 


<210>  5
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human CD8 stalk

<400>  5

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45      


<210>  6
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human IgG1 hinge

<400>  6

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Lys Asp Pro Lys 
            20  


<210>  7
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane derived from human common gamma chain

<400>  7

Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu Leu Cys 
1               5                   10                  15      


Val Tyr Phe Trp Leu 
            20      


<210>  8
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane derived from human IL-2Rbeta

<400>  8

Ile Pro Trp Leu Gly His Leu Leu Val Gly Leu Ser Gly Ala Phe Gly 
1               5                   10                  15      


Phe Ile Ile Leu Val Tyr Leu Leu Ile 
            20                  25  


<210>  9
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane derived from human IL-7Ralpha

<400>  9

Pro Ile Leu Leu Thr Ile Ser Ile Leu Ser Phe Phe Ser Val Ala Leu 
1               5                   10                  15      


Leu Val Ile Leu Ala Cys Val Leu Trp 
            20                  25  


<210>  10
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane derived from human IL-15Ralpha

<400>  10

Ala Ile Ser Thr Ser Thr Val Leu Leu Cys Gly Leu Ser Ala Val Ser 
1               5                   10                  15      


Leu Leu Ala Cys Tyr Leu 
            20          


<210>  11
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv based on J591

<400>  11

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Thr 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Glu Tyr 
            20                  25                  30          


Thr Ile His Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Asn Pro Asn Asn Gly Gly Thr Thr Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Glu Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gly Trp Asn Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr 
            100                 105                 110         


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser 
    130                 135                 140                 


Val Gly Asp Arg Val Ser Ile Ile Cys Lys Ala Ser Gln Asp Val Gly 
145                 150                 155                 160 


Thr Ala Val Asp Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu 
                165                 170                 175     


Leu Ile Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe 
            180                 185                 190         


Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Asn Val 
        195                 200                 205             


Gln Ser Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Ser Tyr 
    210                 215                 220                 


Pro Leu Thr Phe Gly Ala Gly Thr Met Leu Asp Leu Lys Arg 
225                 230                 235             


<210>  12
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3 Z endodomain

<400>  12

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  13
<211>  152
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 and CD3 Zeta endodomains

<400>  13

Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
1               5                   10                  15      


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
            20                  25                  30          


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala 
        35                  40                  45              


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
    50                  55                  60                  


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
65                  70                  75                  80  


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
                85                  90                  95      


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
            100                 105                 110         


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
        115                 120                 125             


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
    130                 135                 140                 


His Met Gln Ala Leu Pro Pro Arg 
145                 150         


<210>  14
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28, OX40 and CD3 Zeta endodomains

<400>  14

Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
1               5                   10                  15      


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
            20                  25                  30          


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro Pro Asp 
        35                  40                  45              


Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu 
    50                  55                  60                  


Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe 
65                  70                  75                  80  


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
                85                  90                  95      


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
            100                 105                 110         


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
        115                 120                 125             


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
    130                 135                 140                 


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
145                 150                 155                 160 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
                165                 170                 175     


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            180                 185             


<210>  15
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ICOS endodomain

<400>  15

Cys Trp Leu Thr Lys Lys Lys Tyr Ser Ser Ser Val His Asp Pro Asn 
1               5                   10                  15      


Gly Glu Tyr Met Phe Met Arg Ala Val Asn Thr Ala Lys Lys Ser Arg 
            20                  25                  30          


Leu Thr Asp Val Thr Leu 
        35              


<210>  16
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD27 endodomain

<400>  16

Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val Glu Pro 
1               5                   10                  15      


Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu Gly Ser Thr 
            20                  25                  30          


Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys Ser Pro 
        35                  40                  45              


<210>  17
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BTLA endodomain

<400>  17

Arg Arg His Gln Gly Lys Gln Asn Glu Leu Ser Asp Thr Ala Gly Arg 
1               5                   10                  15      


Glu Ile Asn Leu Val Asp Ala His Leu Lys Ser Glu Gln Thr Glu Ala 
            20                  25                  30          


Ser Thr Arg Gln Asn Ser Gln Val Leu Leu Ser Glu Thr Gly Ile Tyr 
        35                  40                  45              


Asp Asn Asp Pro Asp Leu Cys Phe Arg Met Gln Glu Gly Ser Glu Val 
    50                  55                  60                  


Tyr Ser Asn Pro Cys Leu Glu Glu Asn Lys Pro Gly Ile Val Tyr Ala 
65                  70                  75                  80  


Ser Leu Asn His Ser Val Ile Gly Pro Asn Ser Arg Leu Ala Arg Asn 
                85                  90                  95      


Val Lys Glu Ala Pro Thr Glu Tyr Ala Ser Ile Cys Val Arg Ser 
            100                 105                 110     


<210>  18
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD30 endodomain

<400>  18

His Arg Arg Ala Cys Arg Lys Arg Ile Arg Gln Lys Leu His Leu Cys 
1               5                   10                  15      


Tyr Pro Val Gln Thr Ser Gln Pro Lys Leu Glu Leu Val Asp Ser Arg 
            20                  25                  30          


Pro Arg Arg Ser Ser Thr Gln Leu Arg Ser Gly Ala Ser Val Thr Glu 
        35                  40                  45              


Pro Val Ala Glu Glu Arg Gly Leu Met Ser Gln Pro Leu Met Glu Thr 
    50                  55                  60                  


Cys His Ser Val Gly Ala Ala Tyr Leu Glu Ser Leu Pro Leu Gln Asp 
65                  70                  75                  80  


Ala Ser Pro Ala Gly Gly Pro Ser Ser Pro Arg Asp Leu Pro Glu Pro 
                85                  90                  95      


Arg Val Ser Thr Glu His Thr Asn Asn Lys Ile Glu Lys Ile Tyr Ile 
            100                 105                 110         


Met Lys Ala Asp Thr Val Ile Val Gly Thr Val Lys Ala Glu Leu Pro 
        115                 120                 125             


Glu Gly Arg Gly Leu Ala Gly Pro Ala Glu Pro Glu Leu Glu Glu Glu 
    130                 135                 140                 


Leu Glu Ala Asp His Thr Pro His Tyr Pro Glu Gln Glu Thr Glu Pro 
145                 150                 155                 160 


Pro Leu Gly Ser Cys Ser Asp Val Met Leu Ser Val Glu Glu Glu Gly 
                165                 170                 175     


Lys Glu Asp Pro Leu Pro Thr Ala Ala Ser Gly Lys 
            180                 185             


<210>  19
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GITR endodomain

<400>  19

Gln Leu Gly Leu His Ile Trp Gln Leu Arg Ser Gln Cys Met Trp Pro 
1               5                   10                  15      


Arg Glu Thr Gln Leu Leu Leu Glu Val Pro Pro Ser Thr Glu Asp Ala 
            20                  25                  30          


Arg Ser Cys Gln Phe Pro Glu Glu Glu Arg Gly Glu Arg Ser Ala Glu 
        35                  40                  45              


Glu Lys Gly Arg Leu Gly Asp Leu Trp Val 
    50                  55              


<210>  20
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HVEM endodomain

<400>  20

Cys Val Lys Arg Arg Lys Pro Arg Gly Asp Val Val Lys Val Ile Val 
1               5                   10                  15      


Ser Val Gln Arg Lys Arg Gln Glu Ala Glu Gly Glu Ala Thr Val Ile 
            20                  25                  30          


Glu Ala Leu Gln Ala Pro Pro Asp Val Thr Thr Val Ala Val Glu Glu 
        35                  40                  45              


Thr Ile Pro Ser Phe Thr Gly Arg Ser Pro Asn His 
    50                  55                  60  


<210>  21
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  21

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  22
<211>  619
<212>  PRT
<213>  Homo sapiens

<400>  22

Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser 
1               5                   10                  15      


Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly 
            20                  25                  30          


Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu 
        35                  40                  45              


Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln 
    50                  55                  60                  


Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro 
65                  70                  75                  80  


Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys 
                85                  90                  95      


Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro 
            100                 105                 110         


Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg 
        115                 120                 125             


Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser 
    130                 135                 140                 


Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg 
145                 150                 155                 160 


Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys 
                165                 170                 175     


Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg 
            180                 185                 190         


Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val 
        195                 200                 205             


Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro 
    210                 215                 220                 


Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys 
225                 230                 235                 240 


Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn 
                245                 250                 255     


Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala 
            260                 265                 270         


His Pro Ser Thr Leu Thr His Pro Gln Arg Arg Ile Asp Thr Leu Asn 
        275                 280                 285             


Ser Asp Gly Tyr Thr Pro Glu Pro Ala Arg Ile Thr Ser Pro Asp Lys 
    290                 295                 300                 


Pro Arg Pro Met Pro Met Asp Thr Ser Val Tyr Glu Ser Pro Tyr Ser 
305                 310                 315                 320 


Asp Pro Glu Glu Leu Lys Asp Lys Lys Leu Phe Leu Lys Arg Asp Asn 
                325                 330                 335     


Leu Leu Ile Ala Asp Ile Glu Leu Gly Cys Gly Asn Phe Gly Ser Val 
            340                 345                 350         


Arg Gln Gly Val Tyr Arg Met Arg Lys Lys Gln Ile Asp Val Ala Ile 
        355                 360                 365             


Lys Val Leu Lys Gln Gly Thr Glu Lys Ala Asp Thr Glu Glu Met Met 
    370                 375                 380                 


Arg Glu Ala Gln Ile Met His Gln Leu Asp Asn Pro Tyr Ile Val Arg 
385                 390                 395                 400 


Leu Ile Gly Val Cys Gln Ala Glu Ala Leu Met Leu Val Met Glu Met 
                405                 410                 415     


Ala Gly Gly Gly Pro Leu His Lys Phe Leu Val Gly Lys Arg Glu Glu 
            420                 425                 430         


Ile Pro Val Ser Asn Val Ala Glu Leu Leu His Gln Val Ser Met Gly 
        435                 440                 445             


Met Lys Tyr Leu Glu Glu Lys Asn Phe Val His Arg Asp Leu Ala Ala 
    450                 455                 460                 


Arg Asn Val Leu Leu Val Asn Arg His Tyr Ala Lys Ile Ser Asp Phe 
465                 470                 475                 480 


Gly Leu Ser Lys Ala Leu Gly Ala Asp Asp Ser Tyr Tyr Thr Ala Arg 
                485                 490                 495     


Ser Ala Gly Lys Trp Pro Leu Lys Trp Tyr Ala Pro Glu Cys Ile Asn 
            500                 505                 510         


Phe Arg Lys Phe Ser Ser Arg Ser Asp Val Trp Ser Tyr Gly Val Thr 
        515                 520                 525             


Met Trp Glu Ala Leu Ser Tyr Gly Gln Lys Pro Tyr Lys Lys Met Lys 
    530                 535                 540                 


Gly Pro Glu Val Met Ala Phe Ile Glu Gln Gly Lys Arg Met Glu Cys 
545                 550                 555                 560 


Pro Pro Glu Cys Pro Pro Glu Leu Tyr Ala Leu Met Ser Asp Cys Trp 
                565                 570                 575     


Ile Tyr Lys Trp Glu Asp Arg Pro Asp Phe Leu Thr Val Glu Gln Arg 
            580                 585                 590         


Met Arg Ala Cys Tyr Tyr Ser Leu Ala Ser Lys Val Glu Gly Pro Pro 
        595                 600                 605             


Gly Ser Thr Gln Lys Ala Glu Ala Ala Cys Ala 
    610                 615                 


<210>  23
<211>  280
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70 SH2 domain

<400>  23

Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser 
1               5                   10                  15      


Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly 
            20                  25                  30          


Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu 
        35                  40                  45              


Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln 
    50                  55                  60                  


Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro 
65                  70                  75                  80  


Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys 
                85                  90                  95      


Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro 
            100                 105                 110         


Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg 
        115                 120                 125             


Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser 
    130                 135                 140                 


Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg 
145                 150                 155                 160 


Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys 
                165                 170                 175     


Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg 
            180                 185                 190         


Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val 
        195                 200                 205             


Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro 
    210                 215                 220                 


Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys 
225                 230                 235                 240 


Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn 
                245                 250                 255     


Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala 
            260                 265                 270         


His Pro Ser Thr Leu Thr His Pro 
        275                 280 


<210>  24
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Type I cytokine receptor motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  24

Trp Ser Xaa Trp Ser 
1               5   


<210>  25
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  furin cleavage site


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa can be Arg or Lys

<400>  25

Arg Xaa Xaa Arg 
1               


<210>  26
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus Tobacco Etch Virus (TEV) cleavage site

<400>  26

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  27
<211>  1166
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence for a dual CR system

<400>  27

Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu His Ala 
1               5                   10                  15      


Ala Arg Pro Asp Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Val 
            20                  25                  30          


Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ser Ser Gln Ser Leu 
        35                  40                  45              


Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Gln Gln Lys Pro 
    50                  55                  60                  


Gly Gln Ala Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser 
65                  70                  75                  80  


Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Ala Glu Phe Thr 
                85                  90                  95      


Leu Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe Ala Val Tyr Tyr Cys 
            100                 105                 110         


Ser Gln Ser Thr His Val Pro Trp Thr Phe Gly Gln Gly Thr Arg Leu 
        115                 120                 125             


Glu Ile Lys Arg Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys 
145                 150                 155                 160 


Lys Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr 
                165                 170                 175     


Phe Thr Asn Tyr Trp Ile Asn Trp Val Arg Gln Ala Pro Gly Gln Gly 
            180                 185                 190         


Leu Glu Trp Met Gly Asn Ile Tyr Pro Ser Asp Ser Phe Thr Asn Tyr 
        195                 200                 205             


Asn Gln Lys Phe Lys Asp Arg Val Thr Ile Thr Ala Asp Lys Ser Thr 
    210                 215                 220                 


Ser Thr Val Tyr Leu Glu Leu Arg Asn Leu Arg Ser Asp Asp Thr Ala 
225                 230                 235                 240 


Val Tyr Tyr Cys Thr Arg Asp Thr Gln Glu Arg Ser Trp Tyr Phe Asp 
                245                 250                 255     


Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser Asp Pro Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp Val Val Ile 
305                 310                 315                 320 


Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu Leu Cys Val Tyr Phe 
                325                 330                 335     


Trp Leu Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys Asn Leu Glu 
            340                 345                 350         


Asp Leu Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp Ser Gly Val 
        355                 360                 365             


Ser Lys Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser Glu Arg Leu 
    370                 375                 380                 


Cys Leu Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu Gly Glu Gly 
385                 390                 395                 400 


Pro Gly Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp Ala Pro Pro 
                405                 410                 415     


Cys Tyr Thr Leu Lys Pro Glu Thr Arg Arg Val Lys Phe Ser Arg Ser 
            420                 425                 430         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        435                 440                 445             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    450                 455                 460                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
465                 470                 475                 480 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                485                 490                 495     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            500                 505                 510         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        515                 520                 525             


Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly Ser 
    530                 535                 540                 


Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Leu Pro Val 
545                 550                 555                 560 


Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu His Ala Ala Arg Pro 
                565                 570                 575     


Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
            580                 585                 590         


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ala Phe Ser Ile Tyr 
        595                 600                 605             


Asp Met Ser Trp Val Arg Gln Val Pro Gly Lys Gly Leu Glu Trp Val 
    610                 615                 620                 


Ser Tyr Ile Ser Ser Gly Gly Gly Thr Thr Tyr Tyr Pro Asp Thr Val 
625                 630                 635                 640 


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Arg Asn Thr Leu Asp 
                645                 650                 655     


Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr Cys 
            660                 665                 670         


Ala Arg His Ser Gly Tyr Gly Ser Ser Tyr Gly Val Leu Phe Ala Tyr 
        675                 680                 685             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
    690                 695                 700                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln 
705                 710                 715                 720 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                725                 730                 735     


Cys Arg Ala Ser Gln Asp Ile Ser Asn Tyr Leu Asn Trp Leu Gln Gln 
            740                 745                 750         


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Tyr Thr Ser Ile Leu 
        755                 760                 765             


His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu 
    770                 775                 780                 


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
785                 790                 795                 800 


Tyr Cys Gln Gln Gly Asn Thr Leu Pro Trp Thr Phe Gly Gln Gly Thr 
                805                 810                 815     


Lys Leu Glu Ile Lys Arg Ser Pro Ala Glu Pro Lys Ser Pro Asp Lys 
            820                 825                 830         


Thr His Thr Cys Pro Pro Cys Pro Lys Asp Pro Lys Ala Cys Asp Ile 
        835                 840                 845             


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Leu Gly His Leu Leu 
    850                 855                 860                 


Val Gly Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu Leu 
865                 870                 875                 880 


Ile Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys 
                885                 890                 895     


Asn Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His 
            900                 905                 910         


Gly Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser 
        915                 920                 925             


Phe Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu 
    930                 935                 940                 


Glu Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro 
945                 950                 955                 960 


Glu Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr 
                965                 970                 975     


Asn Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu 
            980                 985                 990         


Ala Cys Gln Val Tyr Phe Thr Tyr  Asp Pro Tyr Ser Glu  Glu Asp Pro 
        995                 1000                 1005             


Asp Glu  Gly Val Ala Gly Ala  Pro Thr Gly Ser Ser  Pro Gln Pro 
    1010                 1015                 1020             


Leu Gln  Pro Leu Ser Gly Glu  Asp Asp Ala Tyr Cys  Thr Phe Pro 
    1025                 1030                 1035             


Ser Arg  Asp Asp Leu Leu Leu  Phe Ser Pro Ser Leu  Leu Gly Gly 
    1040                 1045                 1050             


Pro Ser  Pro Pro Ser Thr Ala  Pro Gly Gly Ser Gly  Ala Gly Glu 
    1055                 1060                 1065             


Glu Arg  Met Pro Pro Ser Leu  Gln Glu Arg Val Pro  Arg Asp Trp 
    1070                 1075                 1080             


Asp Pro  Gln Pro Leu Gly Pro  Pro Thr Pro Gly Val  Pro Asp Leu 
    1085                 1090                 1095             


Val Asp  Phe Gln Pro Pro Pro  Glu Leu Val Leu Arg  Glu Ala Gly 
    1100                 1105                 1110             


Glu Glu  Val Pro Asp Ala Gly  Pro Arg Glu Gly Val  Ser Phe Pro 
    1115                 1120                 1125             


Trp Ser  Arg Pro Pro Gly Gln  Gly Glu Phe Arg Ala  Leu Asn Ala 
    1130                 1135                 1140             


Arg Leu  Pro Leu Asn Thr Asp  Ala Tyr Leu Ser Leu  Gln Glu Leu 
    1145                 1150                 1155             


Gln Gly  Gln Asp Pro Thr His  Leu 
    1160                 1165     


