               SEQUENCE LISTING

<110> The Royal Veterinary College

<120> Methods

<130> RVCBY/P61986PC

<140> GBPCT/GB2016/053278
<141> 2016-10-20

<150> GB1518762.8
<151> 2015-10-22

<160> 9

<170> BiSSAP 1.3.6

<210> 1
<211> 1386
<212> PRT
<213> Porcine epidemic diarrhea virus


<400> 1
Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 
1               5                   10                  15      
Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 
            20                  25                  30          
Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 
        35                  40                  45              
Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 
    50                  55                  60                  
Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 
65                  70                  75                  80  
Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 
                85                  90                  95      
Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 
            100                 105                 110         
Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 
        115                 120                 125             
Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 
    130                 135                 140                 
Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 
145                 150                 155                 160 
His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 
                165                 170                 175     
Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 
            180                 185                 190         
Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 
        195                 200                 205             
Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 
    210                 215                 220                 
Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 
225                 230                 235                 240 
Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 
                245                 250                 255     
Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys 
            260                 265                 270         
Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 
        275                 280                 285             
Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 
    290                 295                 300                 
Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Pro Arg 
305                 310                 315                 320 
Phe Asn Ile Asn Asp Ile Ser Val Ile Leu Ala Glu Gly Ser Ile Val 
                325                 330                 335     
Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 
            340                 345                 350         
Ser Asn Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln 
        355                 360                 365             
Val Pro Tyr Tyr Cys Phe Phe Lys Val Asp Thr Tyr Asn Ser Thr Val 
    370                 375                 380                 
Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 
385                 390                 395                 400 
Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 
                405                 410                 415     
Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 
            420                 425                 430         
Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 
        435                 440                 445             
Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 
    450                 455                 460                 
Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 
465                 470                 475                 480 
Asp Asp Gly Phe Tyr Thr Ile Ser Ser Arg Asn Leu Leu Ser His Glu 
                485                 490                 495     
Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 
            500                 505                 510         
Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 
        515                 520                 525             
Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 
    530                 535                 540                 
Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 
545                 550                 555                 560 
Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 
                565                 570                 575     
Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 
            580                 585                 590         
Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 
        595                 600                 605             
Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 
    610                 615                 620                 
Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 
625                 630                 635                 640 
Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly 
                645                 650                 655     
Phe Lys Gly Glu Gly Ile Ile Thr Leu Thr Asn Ser Ser Phe Leu Ala 
            660                 665                 670         
Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 
        675                 680                 685             
Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 
    690                 695                 700                 
Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 
705                 710                 715                 720 
Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 
                725                 730                 735     
His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 
            740                 745                 750         
Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 
        755                 760                 765             
Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 
    770                 775                 780                 
Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 
785                 790                 795                 800 
Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 
                805                 810                 815     
Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 
            820                 825                 830         
Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 
        835                 840                 845             
Asn Ser Met Leu Thr Ile Ser Asp Glu Ala Leu Gln Leu Ala Thr Ile 
    850                 855                 860                 
Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 
865                 870                 875                 880 
Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe 
                885                 890                 895     
Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 
            900                 905                 910         
Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 
        915                 920                 925             
Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 
    930                 935                 940                 
Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 
945                 950                 955                 960 
Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 
                965                 970                 975     
Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 
            980                 985                 990         
Gln Arg Asn Gln Gln Leu Leu Ala Glu Ser Phe Asn Ser Ala Ile Gly 
        995                 1000                1005            
Asn Ile Thr Ser Ala Phe Glu Ser Val Lys Glu Ala Ile Ser Gln Thr 
    1010                1015                1020                
Ser Lys Gly Leu Asn Thr Val Ala His Ala Leu Thr Lys Val Gln Glu 
1025                1030                1035                1040
Val Val Asn Ser Gln Gly Ala Ala Leu Thr Gln Leu Thr Val Gln Leu 
                1045                1050                1055    
Gln His Asn Phe Gln Ala Ile Ser Ser Ser Ile Asp Asp Ile Tyr Ser 
            1060                1065                1070        
Arg Leu Asp Ile Leu Ser Ala Asp Ala Gln Val Asp Arg Leu Ile Thr 
        1075                1080                1085            
Gly Arg Leu Ser Ala Leu Asn Ala Phe Val Ala Gln Thr Leu Thr Lys 
    1090                1095                1100                
Tyr Thr Glu Val Gln Ala Ser Arg Lys Leu Ala Gln Gln Lys Val Asn 
1105                1110                1115                1120
Glu Cys Val Lys Ser Gln Ser Gln Arg Tyr Gly Phe Cys Gly Gly Asp 
                1125                1130                1135    
Gly Glu His Ile Phe Ser Leu Val Gln Ala Ala Pro Gln Gly Leu Leu 
            1140                1145                1150        
Phe Leu His Thr Val Leu Val Pro Ser Asp Phe Val Asp Val Ile Ala 
        1155                1160                1165            
Ile Ala Gly Leu Cys Val Asn Asp Glu Ile Ala Leu Thr Leu Arg Glu 
    1170                1175                1180                
Pro Gly Leu Val Leu Phe Thr His Glu Leu Gln Asn His Thr Ala Thr 
1185                1190                1195                1200
Glu Tyr Phe Val Ser Ser Arg Arg Met Phe Glu Pro Arg Lys Pro Thr 
                1205                1210                1215    
Val Ser Asp Phe Val Gln Ile Glu Ser Cys Val Val Thr Tyr Val Asn 
            1220                1225                1230        
Leu Thr Arg Asp Gln Leu Pro Asp Val Ile Pro Asp Tyr Ile Asp Val 
        1235                1240                1245            
Asn Lys Thr Leu Asp Glu Ile Leu Ala Ser Leu Pro Asn Arg Thr Gly 
    1250                1255                1260                
Pro Ser Leu Pro Leu Asp Val Phe Asn Ala Thr Tyr Leu Asn Leu Thr 
1265                1270                1275                1280
Gly Glu Ile Ala Asp Leu Glu Gln Arg Ser Glu Ser Leu Arg Asn Thr 
                1285                1290                1295    
Thr Glu Glu Leu Gln Ser Leu Ile Tyr Asn Ile Asn Asn Thr Leu Val 
            1300                1305                1310        
Asp Leu Glu Trp Leu Asn Arg Val Glu Thr Tyr Ile Lys Trp Pro Trp 
        1315                1320                1325            
Trp Val Trp Leu Ile Ile Phe Ile Val Leu Ile Phe Val Val Ser Leu 
    1330                1335                1340                
Leu Val Phe Cys Cys Ile Ser Thr Gly Cys Cys Gly Cys Cys Gly Cys 
1345                1350                1355                1360
Cys Cys Ala Cys Phe Ser Gly Cys Cys Arg Gly Pro Arg Leu Gln Pro 
                1365                1370                1375    
Tyr Glu Val Phe Glu Lys Val His Val Gln 
            1380                1385    

<210> 2
<211> 4161
<212> DNA
<213> Porcine epidemic diarrhea virus


<400> 2
atgaagtctt taacctactt ctggttgttc ttaccagtac tttcaacact tagcctacca       60

caagatgtca ccaggtgctc agctaacact aattttaggc ggttcttttc aaaatttaat      120

gttcaggcgc ctgcagttgt tgtactgggc ggttatctac ctattggtga aaaccagggt      180

gtcaattcaa cttggtactg tgctggccaa catccaactg ctagtggcgt tcatggtatc      240

tttgttagcc atattagagg tggtcatggc tttgagattg gcatttcgca agagcctttt      300

gaccctagtg gttaccagct ttatttacat aaggctacta acggtaacac taatgctact      360

gcgcgactgc gcatttgcca gtttcctagc attaaaacat tgggccccac tgctaataat      420

gatgttacaa caggtcgtaa ttgcctattt aacaaagcca tcccagctca tatgagtgaa      480

catagtgttg tcggcataac atgggataat gatcgtgtca ctgtcttttc tgacaagatc      540

tattattttt attttaaaaa tgattggtcc cgtgttgcga caaagtgtta caacagtgga      600

ggttgtgcta tgcaatatgt ttacgaaccc acctattaca tgcttaatgt tactagtgct      660

ggtgaggatg gtatttctta tcaaccctgt acagctaatt gcattggtta tgctgccaat      720

gtatttgcta ctgagcccaa tggccacata ccagaaggtt ttagttttaa taattggttt      780

cttttgtcca atgattccac tttggtgcat ggtaaggtgg tttccaacca accattgttg      840

gtcaattgtc ttttggccat tcctaagatt tatggactag gccaattttt ctcctttaat      900

caaacgatcg atggtgtttg taatggagct gctgtgcagc gtgcaccaga ggctccgagg      960

tttaatatta atgacatctc tgtcattctt gctgaaggct caattgtact tcatactgct     1020

ttaggaacaa atttttcttt tgtttgcagt aattcctcaa atcctcactt agccaccttc     1080

gccatacctc tgggtgctac ccaagtacct tattattgtt tttttaaagt ggatacttac     1140

aactccactg tttataaatt tttggctgtt ttacctccta ccgtcaggga aattgtcatc     1200

accaagtatg gtgatgttta tgtcaatggg tttggatact tgcatctcgg tttgttggat     1260

gctgtcacaa ttaatttcac tggtcatggc actgacgatg atgtttctgg tttttggacc     1320

atagcatcga ctaattttgt tgatgcactc atcgaagttc aaggaaccgc cattcagcgt     1380

attctttatt gtgatgatcc tgttagccaa ctcaagtgtt ctcaggttgc ttttgacctt     1440

gacgatggtt tttacactat ttcttctaga aaccttctga gtcatgaaca gccaatttct     1500

tttgttactc tgccatcatt taatgatcat tcttttgtta acattactgt atctgcttcc     1560

tttggtggtc atagtggtgc caaccttatt gcatctgaca ctactatcaa tgggtttagt     1620

tctttctgtg ttgacactag acaatttacc atttcactgt tttataacgt tacaaacagt     1680

tatggttatg tgtctaaatc acaggacagt aattgccctt tcaccttgca atctgttaat     1740

gattacctgt cttttagcaa attttgtgtt tccaccagcc ttttggctag tgcctgtacc     1800

atagatcttt ttggttaccc tgagtttggt agtggtgtta agtttacgtc cctttacttt     1860

caattcacaa agggtgagtt gattactggc acgcctaaac cacttgaagg tgtcacggac     1920

gtttctttta tgactctgga tgtgtgtacc aagtatacta tctatggctt taaaggtgag     1980

ggtatcatta cccttacaaa ttctagcttt ttggcaggtg tttattacac atctgattct     2040

ggacagttgt tagcctttaa gaatgtcact agtggtgctg tttattctgt tacgccatgt     2100

tctttttcag agcaggctgc atatgttgat gatgatatag tgggtgttat ttctagtttg     2160

tctagctcca cttttaacag tactagggag ttgcctggtt tcttctacca ttctaatgat     2220

ggctctaatt gtacagagcc tgtgttggtg tatagtaaca taggtgtttg taaatctggc     2280

agtattggct acgtcccatc tcagtctggc caagtcaaga ttgcacccac ggttactggg     2340

aatattagta ttcccaccaa ctttagtatg agtattagga cagaatattt acagctttac     2400

aacacgcctg ttagtgttga ttgtgccaca tatgtttgta atggtaactc tcgttgtaaa     2460

caattactca cccagtacac tgcagcatgt aagaccatag agtcagcatt acaactcagc     2520

gctaggcttg agtctgttga agttaactct atgcttacta tttctgatga ggctctacag     2580

ttagctacca ttagttcgtt taatggtgat ggatataatt ttactaatgt gctgggtgtt     2640

tctgtgtatg atcctgcaag tggcagggtg gtacaaaaaa ggtcttttat tgaagacctg     2700

ctttttaata aagtggttac taatggcctt ggtactgttg atgaagacta taagcgctgt     2760

tctaatggtc gctctgtggc agatctagtc tgtgcacagt attactctgg tgtcatggta     2820

ctacctggtg ttgttgacgc tgagaagctt cacatgtata gtgcgtctct catcggtggt     2880

atggtgctag gaggttttac ttctgcagcg gcattgcctt ttagctatgc tgttcaagct     2940

agactcaatt atcttgctct acagacggat gttctacagc ggaaccagca attgcttgct     3000

gagtctttta actctgctat tggtaatata acttcagcct ttgagagtgt taaagaggct     3060

attagtcaaa cttccaaggg tttgaacact gtggctcatg cgcttactaa ggttcaagag     3120

gttgttaact cgcagggtgc agctttgact caacttaccg tacagctgca acacaacttc     3180

caagccattt ctagttctat tgatgacatt tactctcgac tggacattct ttcagccgat     3240

gctcaggttg accgtctcat caccggcaga ttatcagcac ttaatgcttt tgttgctcaa     3300

accctcacta agtatactga ggttcaggct agcaggaagt tagcacagca aaaggttaat     3360

gagtgcgtta aatcgcaatc tcagcgttat ggtttttgtg gtggtgatgg cgagcacatt     3420

ttctctctgg tacaggcagc acctcagggc ctgctgtttt tacatacagt acttgtaccg     3480

agtgattttg tagatgttat tgccatcgct ggcttatgcg ttaacgatga aattgccttg     3540

actctacgtg agcctggctt agtcttgttt acgcatgaac ttcaaaatca tactgcgacg     3600

gaatattttg tttcatcgcg acgtatgttt gaacctagaa aacctaccgt tagtgatttt     3660

gttcaaattg agagttgtgt ggtcacctat gtcaatttga ctagagacca actaccagat     3720

gtaatcccag attacatcga tgttaacaaa acacttgatg agattttagc ttctctgccc     3780

aatagaactg gtccaagtct tcctttagat gtttttaatg ccacttatct taatctcact     3840

ggtgaaattg cagatttaga gcagcgttca gagtctctcc gtaatactac agaggagctc     3900

caaagtctta tatataatat caacaacaca ctagttgacc ttgagtggct caaccgagtt     3960

gagacatata tcaagtggcc gtggtgggtt tggttgatta ttttcattgt tctcatcttt     4020

gttgtgtcat tactagtgtt ctgctgcatt tccacgggtt gttgtggatg ctgcggctgc     4080

tgctgtgctt gtttctcagg ttgttgtagg ggtcctagac ttcaacctta cgaagttttt     4140

gaaaaggtcc acgtgcagtg a                                               4161


<210> 3
<211> 1307
<212> PRT
<213> Porcine epidemic diarrhea virus


<400> 3
Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe Arg Arg Phe Phe 
1               5                   10                  15      
Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val Leu Gly Gly Tyr 
            20                  25                  30          
Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr Trp Tyr Cys Ala 
        35                  40                  45              
Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile Phe Val Ser His 
    50                  55                  60                  
Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser Gln Glu Pro Phe 
65                  70                  75                  80  
Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala Thr Asn Gly Asn 
                85                  90                  95      
Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe Pro Ser Ile Lys 
            100                 105                 110         
Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr Gly Arg Asn Cys 
        115                 120                 125             
Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu His Ser Val Val 
    130                 135                 140                 
Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe Ser Asp Lys Ile 
145                 150                 155                 160 
Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val Ala Thr Lys Cys 
                165                 170                 175     
Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr Glu Pro Thr Tyr 
            180                 185                 190         
Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly Ile Ser Tyr Gln 
        195                 200                 205             
Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn Val Phe Ala Thr 
    210                 215                 220                 
Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe Asn Asn Trp Phe 
225                 230                 235                 240 
Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys Val Val Ser Asn 
                245                 250                 255     
Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro Lys Ile Tyr Gly 
            260                 265                 270         
Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp Gly Val Cys Asn 
        275                 280                 285             
Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Pro Arg Phe Asn Ile Asn 
    290                 295                 300                 
Asp Ile Ser Val Ile Leu Ala Glu Gly Ser Ile Val Leu His Thr Ala 
305                 310                 315                 320 
Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser Ser Asn Pro His 
                325                 330                 335     
Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln Val Pro Tyr Tyr 
            340                 345                 350         
Cys Phe Phe Lys Val Asp Thr Tyr Asn Ser Thr Val Tyr Lys Phe Leu 
        355                 360                 365             
Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile Thr Lys Tyr Gly 
    370                 375                 380                 
Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu Gly Leu Leu Asp 
385                 390                 395                 400 
Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp Asp Asp Val Ser 
                405                 410                 415     
Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp Ala Leu Ile Glu 
            420                 425                 430         
Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys Asp Asp Pro Val 
        435                 440                 445             
Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu Asp Asp Gly Phe 
    450                 455                 460                 
Tyr Thr Ile Ser Ser Arg Asn Leu Leu Ser His Glu Gln Pro Ile Ser 
465                 470                 475                 480 
Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe Val Asn Ile Thr 
                485                 490                 495     
Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn Leu Ile Ala Ser 
            500                 505                 510         
Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val Asp Thr Arg Gln 
        515                 520                 525             
Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser Tyr Gly Tyr Val 
    530                 535                 540                 
Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu Gln Ser Val Asn 
545                 550                 555                 560 
Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr Ser Leu Leu Ala 
                565                 570                 575     
Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu Phe Gly Ser Gly 
            580                 585                 590         
Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys Gly Glu Leu Ile 
        595                 600                 605             
Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp Val Ser Phe Met 
    610                 615                 620                 
Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly Phe Lys Gly Glu 
625                 630                 635                 640 
Gly Ile Ile Thr Leu Thr Asn Ser Ser Phe Leu Ala Gly Val Tyr Tyr 
                645                 650                 655     
Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn Val Thr Ser Gly 
            660                 665                 670         
Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu Gln Ala Ala Tyr 
        675                 680                 685             
Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu Ser Ser Ser Thr 
    690                 695                 700                 
Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr His Ser Asn Asp 
705                 710                 715                 720 
Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser Asn Ile Gly Val 
                725                 730                 735     
Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln Ser Gly Gln Val 
            740                 745                 750         
Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile Pro Thr Asn Phe 
        755                 760                 765             
Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr Asn Thr Pro Val 
    770                 775                 780                 
Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn Ser Arg Cys Lys 
785                 790                 795                 800 
Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr Ile Glu Ser Ala 
                805                 810                 815     
Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val Asn Ser Met Leu 
            820                 825                 830         
Thr Ile Ser Asp Glu Ala Leu Gln Leu Ala Thr Ile Ser Ser Phe Asn 
        835                 840                 845             
Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val Ser Val Tyr Asp 
    850                 855                 860                 
Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe Ile Glu Asp Leu 
865                 870                 875                 880 
Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr Val Asp Glu Asp 
                885                 890                 895     
Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp Leu Val Cys Ala 
            900                 905                 910         
Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val Val Asp Ala Glu 
        915                 920                 925             
Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly Met Val Leu Gly 
    930                 935                 940                 
Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr Ala Val Gln Ala 
945                 950                 955                 960 
Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu Gln Arg Asn Gln 
                965                 970                 975     
Gln Leu Leu Ala Glu Ser Phe Asn Ser Ala Ile Gly Asn Ile Thr Ser 
            980                 985                 990         
Ala Phe Glu Ser Val Lys Glu Ala Ile Ser Gln Thr Ser Lys Gly Leu 
        995                 1000                1005            
Asn Thr Val Ala His Ala Leu Thr Lys Val Gln Glu Val Val Asn Ser 
    1010                1015                1020                
Gln Gly Ala Ala Leu Thr Gln Leu Thr Val Gln Leu Gln His Asn Phe 
1025                1030                1035                1040
Gln Ala Ile Ser Ser Ser Ile Asp Asp Ile Tyr Ser Arg Leu Asp Ile 
                1045                1050                1055    
Leu Ser Ala Asp Ala Gln Val Asp Arg Leu Ile Thr Gly Arg Leu Ser 
            1060                1065                1070        
Ala Leu Asn Ala Phe Val Ala Gln Thr Leu Thr Lys Tyr Thr Glu Val 
        1075                1080                1085            
Gln Ala Ser Arg Lys Leu Ala Gln Gln Lys Val Asn Glu Cys Val Lys 
    1090                1095                1100                
Ser Gln Ser Gln Arg Tyr Gly Phe Cys Gly Gly Asp Gly Glu His Ile 
1105                1110                1115                1120
Phe Ser Leu Val Gln Ala Ala Pro Gln Gly Leu Leu Phe Leu His Thr 
                1125                1130                1135    
Val Leu Val Pro Ser Asp Phe Val Asp Val Ile Ala Ile Ala Gly Leu 
            1140                1145                1150        
Cys Val Asn Asp Glu Ile Ala Leu Thr Leu Arg Glu Pro Gly Leu Val 
        1155                1160                1165            
Leu Phe Thr His Glu Leu Gln Asn His Thr Ala Thr Glu Tyr Phe Val 
    1170                1175                1180                
Ser Ser Arg Arg Met Phe Glu Pro Arg Lys Pro Thr Val Ser Asp Phe 
1185                1190                1195                1200
Val Gln Ile Glu Ser Cys Val Val Thr Tyr Val Asn Leu Thr Arg Asp 
                1205                1210                1215    
Gln Leu Pro Asp Val Ile Pro Asp Tyr Ile Asp Val Asn Lys Thr Leu 
            1220                1225                1230        
Asp Glu Ile Leu Ala Ser Leu Pro Asn Arg Thr Gly Pro Ser Leu Pro 
        1235                1240                1245            
Leu Asp Val Phe Asn Ala Thr Tyr Leu Asn Leu Thr Gly Glu Ile Ala 
    1250                1255                1260                
Asp Leu Glu Gln Arg Ser Glu Ser Leu Arg Asn Thr Thr Glu Glu Leu 
1265                1270                1275                1280
Gln Ser Leu Ile Tyr Asn Ile Asn Asn Thr Leu Val Asp Leu Glu Trp 
                1285                1290                1295    
Leu Asn Arg Val Glu Thr Tyr Ile Lys Trp Pro 
            1300                1305        

<210> 4
<211> 3921
<212> DNA
<213> Porcine epidemic diarrhea virus


<400> 4
caagatgtca ccaggtgctc agctaacact aattttaggc ggttcttttc aaaatttaat       60

gttcaggcgc ctgcagttgt tgtactgggc ggttatctac ctattggtga aaaccagggt      120

gtcaattcaa cttggtactg tgctggccaa catccaactg ctagtggcgt tcatggtatc      180

tttgttagcc atattagagg tggtcatggc tttgagattg gcatttcgca agagcctttt      240

gaccctagtg gttaccagct ttatttacat aaggctacta acggtaacac taatgctact      300

gcgcgactgc gcatttgcca gtttcctagc attaaaacat tgggccccac tgctaataat      360

gatgttacaa caggtcgtaa ttgcctattt aacaaagcca tcccagctca tatgagtgaa      420

catagtgttg tcggcataac atgggataat gatcgtgtca ctgtcttttc tgacaagatc      480

tattattttt attttaaaaa tgattggtcc cgtgttgcga caaagtgtta caacagtgga      540

ggttgtgcta tgcaatatgt ttacgaaccc acctattaca tgcttaatgt tactagtgct      600

ggtgaggatg gtatttctta tcaaccctgt acagctaatt gcattggtta tgctgccaat      660

gtatttgcta ctgagcccaa tggccacata ccagaaggtt ttagttttaa taattggttt      720

cttttgtcca atgattccac tttggtgcat ggtaaggtgg tttccaacca accattgttg      780

gtcaattgtc ttttggccat tcctaagatt tatggactag gccaattttt ctcctttaat      840

caaacgatcg atggtgtttg taatggagct gctgtgcagc gtgcaccaga ggctccgagg      900

tttaatatta atgacatctc tgtcattctt gctgaaggct caattgtact tcatactgct      960

ttaggaacaa atttttcttt tgtttgcagt aattcctcaa atcctcactt agccaccttc     1020

gccatacctc tgggtgctac ccaagtacct tattattgtt tttttaaagt ggatacttac     1080

aactccactg tttataaatt tttggctgtt ttacctccta ccgtcaggga aattgtcatc     1140

accaagtatg gtgatgttta tgtcaatggg tttggatact tgcatctcgg tttgttggat     1200

gctgtcacaa ttaatttcac tggtcatggc actgacgatg atgtttctgg tttttggacc     1260

atagcatcga ctaattttgt tgatgcactc atcgaagttc aaggaaccgc cattcagcgt     1320

attctttatt gtgatgatcc tgttagccaa ctcaagtgtt ctcaggttgc ttttgacctt     1380

gacgatggtt tttacactat ttcttctaga aaccttctga gtcatgaaca gccaatttct     1440

tttgttactc tgccatcatt taatgatcat tcttttgtta acattactgt atctgcttcc     1500

tttggtggtc atagtggtgc caaccttatt gcatctgaca ctactatcaa tgggtttagt     1560

tctttctgtg ttgacactag acaatttacc atttcactgt tttataacgt tacaaacagt     1620

tatggttatg tgtctaaatc acaggacagt aattgccctt tcaccttgca atctgttaat     1680

gattacctgt cttttagcaa attttgtgtt tccaccagcc ttttggctag tgcctgtacc     1740

atagatcttt ttggttaccc tgagtttggt agtggtgtta agtttacgtc cctttacttt     1800

caattcacaa agggtgagtt gattactggc acgcctaaac cacttgaagg tgtcacggac     1860

gtttctttta tgactctgga tgtgtgtacc aagtatacta tctatggctt taaaggtgag     1920

ggtatcatta cccttacaaa ttctagcttt ttggcaggtg tttattacac atctgattct     1980

ggacagttgt tagcctttaa gaatgtcact agtggtgctg tttattctgt tacgccatgt     2040

tctttttcag agcaggctgc atatgttgat gatgatatag tgggtgttat ttctagtttg     2100

tctagctcca cttttaacag tactagggag ttgcctggtt tcttctacca ttctaatgat     2160

ggctctaatt gtacagagcc tgtgttggtg tatagtaaca taggtgtttg taaatctggc     2220

agtattggct acgtcccatc tcagtctggc caagtcaaga ttgcacccac ggttactggg     2280

aatattagta ttcccaccaa ctttagtatg agtattagga cagaatattt acagctttac     2340

aacacgcctg ttagtgttga ttgtgccaca tatgtttgta atggtaactc tcgttgtaaa     2400

caattactca cccagtacac tgcagcatgt aagaccatag agtcagcatt acaactcagc     2460

gctaggcttg agtctgttga agttaactct atgcttacta tttctgatga ggctctacag     2520

ttagctacca ttagttcgtt taatggtgat ggatataatt ttactaatgt gctgggtgtt     2580

tctgtgtatg atcctgcaag tggcagggtg gtacaaaaaa ggtcttttat tgaagacctg     2640

ctttttaata aagtggttac taatggcctt ggtactgttg atgaagacta taagcgctgt     2700

tctaatggtc gctctgtggc agatctagtc tgtgcacagt attactctgg tgtcatggta     2760

ctacctggtg ttgttgacgc tgagaagctt cacatgtata gtgcgtctct catcggtggt     2820

atggtgctag gaggttttac ttctgcagcg gcattgcctt ttagctatgc tgttcaagct     2880

agactcaatt atcttgctct acagacggat gttctacagc ggaaccagca attgcttgct     2940

gagtctttta actctgctat tggtaatata acttcagcct ttgagagtgt taaagaggct     3000

attagtcaaa cttccaaggg tttgaacact gtggctcatg cgcttactaa ggttcaagag     3060

gttgttaact cgcagggtgc agctttgact caacttaccg tacagctgca acacaacttc     3120

caagccattt ctagttctat tgatgacatt tactctcgac tggacattct ttcagccgat     3180

gctcaggttg accgtctcat caccggcaga ttatcagcac ttaatgcttt tgttgctcaa     3240

accctcacta agtatactga ggttcaggct agcaggaagt tagcacagca aaaggttaat     3300

gagtgcgtta aatcgcaatc tcagcgttat ggtttttgtg gtggtgatgg cgagcacatt     3360

ttctctctgg tacaggcagc acctcagggc ctgctgtttt tacatacagt acttgtaccg     3420

agtgattttg tagatgttat tgccatcgct ggcttatgcg ttaacgatga aattgccttg     3480

actctacgtg agcctggctt agtcttgttt acgcatgaac ttcaaaatca tactgcgacg     3540

gaatattttg tttcatcgcg acgtatgttt gaacctagaa aacctaccgt tagtgatttt     3600

gttcaaattg agagttgtgt ggtcacctat gtcaatttga ctagagacca actaccagat     3660

gtaatcccag attacatcga tgttaacaaa acacttgatg agattttagc ttctctgccc     3720

aatagaactg gtccaagtct tcctttagat gtttttaatg ccacttatct taatctcact     3780

ggtgaaattg cagatttaga gcagcgttca gagtctctcc gtaatactac agaggagctc     3840

caaagtctta tatataatat caacaacaca ctagttgacc ttgagtggct caaccgagtt     3900

gagacatata tcaagtggcc g                                               3921


<210> 5
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward primer to amplify ORF2 from a PCV2b strain (GenBank
      accession number JX193799)

<400> 5
ggtaccaatg acgtatcca                                                 19


<210> 6
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse primer to amplify ORF2 from a PCV2b strain (GenBank
      accession number JX193799)

<400> 6
ctcgagaggg ttaagtgg                                                  18


<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward primer for PCV2 ORF1

<400> 7
gctctytatc ggaggattac                                                20


<210> 8
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse primer for PCV2 ORF1

<400> 8
ataaaaacca ttacgawgtg ata                                            23


<210> 9
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR TaqMan probe for PCV2 ORF1

<400> 9
ccatgccctg aatttccata tgaaat                                         26


