                         SEQUENCE LISTING

<110>  UNIVERSITE DE BORDEAUX
       INSTITUT POLYTECHNIQUE DE BORDEAUX
       ECOLE NATIONALE SUPERIEURE DES SCIENCES AGRONOMIQUES DE 
       BORDEAUX AQUITAINE BORDEAUX SCIENCES AGRO
 
<120>  PROCD D'ANALYSE D'UN CHANTILLON POUR LA PRSENCE DE LEVURES DE
        L'ESPCE BRETTANOMYCES BRUXELLENSIS RSISTANTES AUX SULFITES ET 
       KIT POUR SA MISE EN OEUVRE

<130>  31630 WO

<150>  FR 1559975
<151>  2015-10-20

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1020
<212>  PRT
<213>  Brettanomyces bruxellensis


<220>
<221>  misc_feature
<222>  (347)..(347)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (550)..(550)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (562)..(562)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (600)..(600)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (612)..(612)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (617)..(617)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (695)..(695)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (770)..(770)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (779)..(779)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (889)..(889)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (946)..(946)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (980)..(980)
<223>  Xaa can be any naturally occurring amino acid

<400>  1

Met Arg Leu Met Pro Gln Lys Lys Arg Asp Lys Lys Ser Leu Ser Gly 
1               5                   10                  15      


Val Glu His Val Asn Pro Asp Asn Gly Gly Asp Asp Ser Gly Glu Gly 
            20                  25                  30          


Asp Gly Asn Asx Asp Asn Ala Asp Asp Asp Asp Asp Asp Asp Asp Val 
        35                  40                  45              


Asn Met Thr Met Met Asn Asp Glu Ser Tyr Tyr Ala Asp Gly Ser Val 
    50                  55                  60                  


Glu Glu Gly Arg Val Leu Asn Ser Ser Val Asp Asn Asp Leu Tyr Leu 
65                  70                  75                  80  


Lys Pro Gly Phe Gly Ser Ala Arg Asn Leu Cys Ile Thr Leu Arg Tyr 
                85                  90                  95      


Leu Leu Tyr Glu Gln Ala Val Asp Tyr Phe Tyr Asp Glu Phe Asn Asn 
            100                 105                 110         


Arg Asp Ser Lys Phe His Asp Phe Glu Leu Leu Asp Glu Gln His Asp 
        115                 120                 125             


Ala Asn Lys Asp Glu Thr Ser Ala Lys Asn Leu His Asp Leu Ser Glu 
    130                 135                 140                 


Ser Ser Glu Asp Asn Val Ser Asn Thr Glu Asn Asp Ala Glu Asp Asp 
145                 150                 155                 160 


Asn Tyr Asp Asp Asp Ser Pro Lys Ile Glu Arg Gly Asp Ala Ser Pro 
                165                 170                 175     


Glu Asp Asp Asp Tyr Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Lys 
            180                 185                 190         


Gly Asp Glu Asp Asn Thr Lys Asp Thr Asn Asn Asp Asn Ser Val Lys 
        195                 200                 205             


Val Ile Ser Thr Gly Ile Pro Val Gly Val Asn Asp Lys Gly Ser Glu 
    210                 215                 220                 


Glu Asp Lys Ser Leu Asn Asp Glu Asn Ser Lys Val Asp Asp Glu Asn 
225                 230                 235                 240 


Asp Val Ser Pro Pro Lys Tyr Ile Glu Pro Gly Ser His Gly Glu Met 
                245                 250                 255     


Ile Leu Lys Val Pro Glu Ser Ala Ile Leu Ser Lys Pro Ser Tyr Pro 
            260                 265                 270         


Ser Leu Pro Asn Ser Phe Ser Pro Ala Asp Glu Ala Ile Gly Lys Val 
        275                 280                 285             


Val His Pro Ile Leu Ser Thr Ser Ser Ala Ile Glu Ser Ser Leu Glu 
    290                 295                 300                 


Lys Gln Asn Glu Leu Lys Leu Ile Lys Asn Phe Asn Lys Ile Tyr His 
305                 310                 315                 320 


Gly Phe Asp Asp Asp Leu Gln Asn Ile Val Lys Arg Arg Lys Leu Glu 
                325                 330                 335     


Lys Ser Asn Gln Gln Leu Gly Leu Glu Ser Xaa Glu Ser Asn Ser Ser 
            340                 345                 350         


Lys Thr Asp Gly Lys Leu Asp Gln Ser Val Ser Lys Leu Met Ser Leu 
        355                 360                 365             


Gly Gly Ala Ala Asn Leu Ser Leu Lys Asn Leu Leu Gly Arg Ile Glu 
    370                 375                 380                 


Asp Asn Arg Ser Lys Leu Asn Ile Thr Asp Ile Glu Leu Lys Asn Leu 
385                 390                 395                 400 


Ile Met Asp Val Arg Lys Asn Arg Ser Lys Trp Ala Asn Tyr Asn Arg 
                405                 410                 415     


Ile Gly Gln Glu Glu Leu Tyr Glu Ala Cys Glu Lys Val Val Thr Glu 
            420                 425                 430         


Leu Arg Gly Tyr Thr Glu His Ser Thr Pro Phe Leu Asn Arg Val Ser 
        435                 440                 445             


Lys Arg Glu Ala Pro Asn Tyr Tyr Glu Ile Ile Lys Lys Pro Met Asp 
    450                 455                 460                 


Leu Asn Thr Val Met Arg Lys Leu Arg Asn Phe Gln Tyr Arg Ser Lys 
465                 470                 475                 480 


Lys Gln Phe Val Asp Asp Leu Met Leu Ile Trp Gln Asn Cys Leu Thr 
                485                 490                 495     


Tyr Asn Thr Asp Pro Lys His Tyr Leu Arg Val Asp Ala Ile Ala Met 
            500                 505                 510         


Gln Lys Lys Thr Gln Ser Leu Ile Pro Leu Ile Pro Asp Ile Val Val 
        515                 520                 525             


Arg Asp Arg Ala Glu Val Glu Lys Glu Ala Ala Leu Ala Ala Lys Gln 
    530                 535                 540                 


Asp Lys Glu Glu Gln Xaa Ser Glu Gln Glu Thr Glu Asn Ala Thr Arg 
545                 550                 555                 560 


Ser Xaa Arg Ala Val Gly Ala Val Gly Thr Gly Ser Lys Gln Thr Ala 
                565                 570                 575     


Lys Lys His Arg Lys Gly Pro Thr Gln Glu Asp Asn Gln Glu Glu Val 
            580                 585                 590         


Glu Ser Lys Glu Lys Thr Ala Xaa Leu Asp Ser Lys Thr Glu Glu Glu 
        595                 600                 605             


Thr Thr Thr Xaa Ser Ala Thr Pro Xaa Gly Thr Asp Thr Pro Ser Thr 
    610                 615                 620                 


Ala His Asp Glu Lys His Leu Pro Ile Asn Leu Thr Lys Ser Val Ser 
625                 630                 635                 640 


Lys Val Asp Ser Met Thr Pro Ala Thr Ala Ser Gly Ala Ser Gly Thr 
                645                 650                 655     


Pro Pro Gln Asn Ile Asp Glu Glu Asn Asp Glu Leu Asp Glu Ala Leu 
            660                 665                 670         


Glu Glu Leu Arg Thr Glu Arg Asn Glu Asp Asp Asp Asp Asn Asp Val 
        675                 680                 685             


Ile Glu Asp Leu Glu Ala Xaa Thr Trp Lys Thr Leu Thr Ala Asn Thr 
    690                 695                 700                 


Arg Tyr Lys Leu Cys Glu Ala Arg Gly Lys Met Phe Lys Gly Asn Lys 
705                 710                 715                 720 


Ile Asp Pro Asn Val Glu Ser Leu Tyr Arg Asp Glu Asn Gln Met Ala 
                725                 730                 735     


Asn Phe Met Asn Tyr Leu Thr Asp Asn Gly Asp Leu Val Ile His Lys 
            740                 745                 750         


Asn Arg Tyr Phe Asp Glu His Asp Asp Pro Tyr Leu Ile Glu Tyr Asp 
        755                 760                 765             


Val Xaa Gly Gly Val Pro Pro Ile Lys Phe Xaa Gly Val Asn Phe Glu 
    770                 775                 780                 


Gln Glu Glu Asn Ala Met Met Glu Lys Leu Leu Gly Lys Gly Glu Thr 
785                 790                 795                 800 


Leu Asp Ser Leu Pro Asp Ser Leu Leu Lys Val Lys Ile Lys Gly Ser 
                805                 810                 815     


Asn Ser Val Ile Met Asp Asn Ile Lys Thr Met Gln Gln Ile Arg Lys 
            820                 825                 830         


Thr Cys Phe Arg Ile Asn Leu Ile Arg Gln Met Gln Thr Ser Gln Phe 
        835                 840                 845             


Val His Arg Ser Gln Met Ser Ala Pro Asp Ile Lys Glu Ile Asn Phe 
    850                 855                 860                 


Asn Asp Ile Asp Pro Leu Ser Lys Leu Pro Thr Arg Asp Ser Leu Asn 
865                 870                 875                 880 


Gly Glu Val Ala Phe Arg Ser Leu Xaa Lys Ser Ile Ser Ala Ile Leu 
                885                 890                 895     


Met Ala Thr Gly Phe Glu Lys Thr Ser Pro Phe Cys Ala Ser Leu Met 
            900                 905                 910         


Thr Gln Leu Ala Glu Glu His Leu Gly Asn Leu Ala Lys Ser Met Lys 
        915                 920                 925             


Leu His Glu Glu Ser Asn Ser Ile Asn Lys Met Asn Ile Arg Gly Lys 
    930                 935                 940                 


Ser Xaa Thr Asn Ile Lys Asn Ile Val Gln Met Ser Leu Glu Glu Asn 
945                 950                 955                 960 


Gly Ile Asp Lys Pro Asp Ile Ile Tyr Thr Tyr Tyr Lys Glu Tyr Leu 
                965                 970                 975     


Ala Lys Arg Xaa Lys Lys Leu Lys Asp Leu Lys Thr Ala Leu Glu Glu 
            980                 985                 990         


Phe Leu Lys Glu Leu Leu Arg Pro  Gly Leu Gln Asp Ile  Asn Glu Thr 
        995                 1000                 1005             


Gln Phe  Thr Asp Asn Ser Asp  Gln Phe Tyr Asp Trp  
    1010                 1015                 1020 


<210>  2
<211>  5063
<212>  DNA
<213>  Brettanomyces bruxellensis

<400>  2
cttattattg gtacaagttt atatatcata aatacaaaca cggattaaag aaataaaaga       60

aataaaaatg aaaaaaaaga aacccatcac acgcctaaaa aaaaggataa gycatgactc      120

tatcacgcrt cacctatgtc ttccgcctta agttttatca gacgaaatat ctttatcatc      180

aatatcaccc tgtgatgcat ccgtgaaaat atctttatcg tcgcctttct cytcatyctc      240

atcgttatca tcttcgccaw cttcattgat tgtrtcaagt atrccatttg aatctttctk      300

ytccgtttca acgctttcct trtgatcgtt atygagtgaa tcatcattag caacttttct      360

aattacagaa tmtccagcat tttctttcga tacctttcca tcggtcacac ttgttgtcgc      420

gtccaattgg ccggaactak atgacttttc ggtatttrtt gtttcwtgcg mttgcgtctt      480

ctycttttct tctgctaccc atttgtcttg atcgtcaata aagaamgaag tagcaatcag      540

tttcttcttt atctgcgtaa tctttccatt aggaggaatt tttggtctat tattcctttg      600

tttaatcggt agatcatcat cctcaataat tctcaaatca ttcaaatctt tatattcaac      660

aaacttttca tttggatctg cagtaccrtt rgcaatatgc tgctggtgtc ttcgtaattg      720

cttggcrtac atagtttcac ttttcgtaag taatcgttca taatagtgct taaggacrcc      780

aatttgtttt ggaagatctt tcttccgaag ctttggaaac wtgaactctt tgaagtcatc      840

atacttctct ttcyttctct tattgttcaa ytggttaaac tgatacgaaa gacgcgagtg      900

yaataaatgc aacggaacac tagcwgaaag aatwccaaat tctttatcca atcccagttc      960

tctgaaacca aagaaatcat ccccaatctc ctcsgaaaat tcaccagtca taaaactgat     1020

cagaattatc agtgaactgy gtttcgttta tatcttgtaa tccaggtctt aacaattcct     1080

tcaagaattc ttccagagca gtcttgagat ctttgagttt cttgytcctt ttagcaagat     1140

attccttgta atacgtgtat attatatcyg gcttatcaat gccattttcc tcaagagaca     1200

tttggacaat atttttgata ttcgttgrag acttacccct aatattcatc ttgttaatgg     1260

arttggactc ttcatgcaat ttcatcgatt ttgcragatt tccaagatgc tcttccgcaa     1320

gttgagtcat taaagaggca caaaawggac ttgttttttc aaatccrgtr gccatgagta     1380

ttgcrgaaat agacttcygt aacgatctga aggcaacttc accattcaac gaatcyctgg     1440

ttgggagctt tgagagcgga tcaatatcat tgaaattaat ttctttgatr tctggtgccg     1500

acatttgcga ccggtgcaca aattgagagg tttgcatctg tctgataaga ttgattctaa     1560

aacatgtctt tcgaatctgt tgcatggtct taatattatc cataataaca ctgttggagc     1620

ccttgatctt cactttcagc aaactgtctg gaagagaatc taaagtttca cctttgccca     1680

gtagcttttc catcatggca ttttcctcct gctcaaaatt cacacctytr aacttaatgg     1740

gcggcactcc accyytaacr tcatattcta tcaaatasgg atcatcrtgy tcatcaaagt     1800

atctattctt gtgaatcacc aaatcgccgt tatccgtaag atagttcatg aaattggcca     1860

tctgattttc gtctctatag agcgactcca catttggatc aattttgttt cctttaaaca     1920

tcttacctct rgcctcacat aacttatayc trgtrttygc agtcaaagtt ttccacgtgg     1980

magcctctaa atcctcaatt acatcattat cgtcatcatc ttcattcctt tctgttcgaa     2040

gttcctccaa agcttcgtct agttcatcgt tttcttcatc aatattttga ggtggagttc     2100

cagaagcacc ggatgctgtt gcaggagtca tggaatcgac cttggataca cttttcgtca     2160

ggtttattgg taaatgcttt tcatcatgyg cagttgatgg rgtatctgtt cccrctggtg     2220

tagcagaaay cgttgtcgtt tcttcttcag tctttgaatc taaagyygcy gtcttttctt     2280

tagattctac ttcttcctga ttgtcttcct gggttggacc ttttcgatgc tttttggccg     2340

tttgcttact tccagttcca acagccccaa cagccctacy agatcttgta gcattctctg     2400

tttcctgctc agawtcttgt tcctccttat cctgttttgc agccaatgca gcttcttttt     2460

ccacttcagc tctatctctc accactatat ccggaatcaa cggtatcaat gactgygttt     2520

tyttctgcat agctattgca tcaacacgaa ggtaatgctt tggatccgtg ttataagtca     2580

aacagttttg ccaaattaac atcaaatcat cyacaaactg ctttttgctt ctatattgga     2640

aatttctcag ctttctcatt actgtattca agtccattgg cttctttatg atttcataat     2700

aattaggagc ttccctttta gagaccctgt ttagaaatgg cgtggaatgc tcagtataac     2760

ctctcaactc agtgacaact ttctcrcagg cttcataaag ctcttcttgc ccaattctat     2820

tgtagtttgc ccattttgat ctattctttc taacatccat tatcaagtty ttgagttcta     2880

tgtctgtaat attaagcttg ctyctgttat cctctattct tcctaataaa ttttttaaag     2940

aaaggttrgc ggctcccccw agactcataa gcttactmac actttgatca agtttkccat     3000

ccgttttact tgaattcgat tcayctgact caagccccaa ttgctgatta ctyttctcaa     3060

gcttccgrcg cttgactata ttttgtagat catcatcaaa accatgatat attttgttaa     3120

agttcttaat caactttagc tcattctgct tctcaagact tgattcaata gcagacgatg     3180

tgctgagaat tggatgaacg acttttccaa ttgcttcatc tgctggtgaa aacgagttcg     3240

gaagacttgg ataacttggc ttggataata tcgcactttc cggaactttt aatatcattt     3300

caccatgtga acctggttca atatatttag gaggagacac atcattttcg tcatctactt     3360

tcgaattttc atcatttaag cttttatctt cctccgatcc tttatcatta actccgactg     3420

ggattcctgt agaaataacc ttaactgaat tatcattatt agtgtccttt gtattatcct     3480

catcaccctt atcatcatca tcatcatcat catcatcatc atagtcatca tcttctggag     3540

aagcatcacc acgttcaatt tttggactgt cgtcatcata attatcatct tctgcatcat     3600

tctcagtatt gcttacattg tcttcagagg attctgacaa atcgtgcaaa ttcttcgcag     3660

atgtttcatc tttattagca tcatgctgtt catccaacaa ctcaaaatca tgaaacttgc     3720

tatctctgtt attaaactcg tcataaaaat aatcaacagc ctgttcataa agaagatatc     3780

gcaatgttat gcaaagattt cgtgcrgaac caaatccagg cttcaagtat aaatcattgt     3840

caactgaact gttgagaaca cgmccttctt caacactscc atcagcataa tatgattcat     3900

cattcatcat agtcatattg acatcatcat catcatcatc atcatcagca ttatcatyat     3960

taccatcacc ttcaccacta tcatcaccac cattatccgg atttacatgt tcaacaccgg     4020

acaaggactt cttatcgcgt tttttttgag gcatcagtcg cattatcatt caaaaattga     4080

acaaaaagat tacagtccat taatgcatcc catgctgcaa gcttttgctc atctacttca     4140

ctctttatag attgatcatc agaggatytc aaaatgaagc taaggaggtt aaacaattgc     4200

ggactaaggt aacaattaaa agaatccttc attgaagagt ttgacggtca accgtcgcag     4260

caacttctta ttatttcctt tgaatgcctc gagtttataa ccggccatta ttttggtctg     4320

ttgaacaaaa aacaattatc caaatacaga tctgaaatat tttaagttga caccaaacga     4380

aattaaaaat tcacccttta actgcaacgt tcttccagaa aagatacccg ggccaaggta     4440

gctaatatat gtgaaatatg attactgtca aggaacacta atggatgaat agtgaaatgt     4500

gatyctggaa gaactttgta gcctaactat aatcatgcag acgtatgagt tacttaaaaa     4560

aaaaaawata ratraataaa tcgaatataa tataaaaaty gasaacaaaa ctactcacct     4620

ctrggcacgc cgatcgacgt agtcyggkyt ttattttatt ttcrgargct attctataca     4680

tattttttgc taatcgagga taaatcatgt gcgtataatr tgggcactag gyaaactgta     4740

ataaytctac cgttttatat attttctttc aaaagtatak aagtttccta ctttcggrca     4800

attmtttgtt tmttggatcr agagtttaag raaggaatac cctrttcaaa agracryagt     4860

satcaattta aacgaatata ttggttagac ctaggaaaga gagctaggaa tagagaaagt     4920

catataaaat gccagatttt gtggataaga catttgatgc agtatattca gtgtatcaga     4980

cagcagtggg tgctctgaat ggagcatatg ataaggccaa ggatgctggg caaagcatta     5040

tggaatatac aaaaaatagt gcc                                             5063


<210>  3
<211>  21
<212>  DNA
<213>  Brettanomyces bruxellensis

<400>  3
tcttcctccg atcctttatc a                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  Brettanomyces bruxellensis

<400>  4
ttgcacgatt tgtcagaatc c                                                 21


<210>  5
<211>  25
<212>  DNA
<213>  Brettanomyces bruxellensis

<400>  5
agaagttgaa cggccgcatt tgcac                                             25


<210>  6
<211>  25
<212>  DNA
<213>  Brettanomyces bruxellensis

<400>  6
aggattgttg acactctcgc cgagg                                             25


<210>  7
<211>  263
<212>  DNA
<213>  Brettanomyces bruxellensis

<400>  7
tcttcctccg atcctttatc attaactccg actgggattc ctgtagaaat aaccttaact       60

gaattatcat tattagtgtc ctttgtatta tcctcatcac ccttatcatc atcatcatca      120

tcatcatcat catcatagtc atcatcttct ggagaagcat caccacgttc aatttttgga      180

ctgtcgtcat cataattatc atcttctgca tcattctcag tattgcttac attgtcttca      240

gaggattctg acaaatcgtg caa                                              263


<210>  8
<211>  39
<212>  DNA
<213>  Brettanomyces bruxellensis

<400>  8
acgacgttgt aaaacgactc ttcctccgat cctttatca                              39


